酶底物法快检技术在四川5.12地震灾区生活饮用水监测中的应用
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摘要
目的:在四川5.12抗震救灾时期,开展了灾区生活饮用水的卫生监测工作,由于时间和人力资源的限制,对微生物快速检测提出了要求。方法:运用酶底物法快速检测生活饮用水中三项微生物指标:大肠菌群、耐热大肠菌群、大肠埃希菌。结果:共检测灾区生活饮用水2000余件,其中市政供水350余件,合格率约94%;乡镇供水1650余件,合格率约90%。酶底物法24 h可报告结果,并能同时检测2个指标,实现了快速检测的目的。结论:酶底物法作为快速检测技术,在此次生活饮用水监测工作中发挥了突出的作用,极大地节约了时间和人力,有力地支持了抗震救灾工作的顺利实施,保障了广大灾区人民群众生活饮用水的供水安全。
Objective:Health monitoring in drinking water in Sichuan 5.12 earthquake-stricken area had been carried out.The rapid detection methods in micro-organisms were needed for time and labor limitations.Methods:Use enzyme substrate technique to detect total coliforms,thermotolerant coliform bacteria and Escherichia coli in drinking water.Results: There were more than 2000 samples have been detected.And in which,350 samples from municipal water supply with a pass rate of about 94%,and 1650 samples from township water supply with a pass rate of about 90%.Enzyme substrate technique is a rapid detection method for it can make results within 24 hours and can detect two items at the same time.Conclusion:The enzyme substrate technique can obviously save time and labor.It has been successfully applied in detecting drinking water in Sichuan 5.12 earthquake-stricken area.
Objective:Health monitoring in drinking water in Sichuan 5.12 earthquake-stricken area had been carried out.The rapid detection methods in micro-organisms were needed for time and labor limitations.Methods:Use enzyme substrate technique to detect total coliforms,thermotolerant coliform bacteria and Escherichia coli in drinking water.Results: There were more than 2000 samples have been detected.And in which,350 samples from municipal water supply with a pass rate of about 94%,and 1650 samples from township water supply with a pass rate of about 90%.Enzyme substrate technique is a rapid detection method for it can make results within 24 hours and can detect two items at the same time.Conclusion:The enzyme substrate technique can obviously save time and labor.It has been successfully applied in detecting drinking water in Sichuan 5.12 earthquake-stricken area.
引文
[1]GB/T5750-2006.生活饮用水标准检验方法[S].
[2]GB/T5749-2006.生活饮用水卫生标准[S].
[3]孙宗科,吴榕,丁培,等.水中大肠菌群快速检测方法-酶底物法与多管发酵法的比较[J].卫生研究,2006,35(4):497-498.
[4]王秀茹.预防医学微生物学及检验技术[M].北京:人民卫生出版社,2002:675-683.
[2]GB/T5749-2006.生活饮用水卫生标准[S].
[3]孙宗科,吴榕,丁培,等.水中大肠菌群快速检测方法-酶底物法与多管发酵法的比较[J].卫生研究,2006,35(4):497-498.
[4]王秀茹.预防医学微生物学及检验技术[M].北京:人民卫生出版社,2002:675-683.
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