AFMBR处理生活污水的功能菌群特性研究
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摘要
厌氧流化床膜生物反应器(AFMBR)作为一种低耗产能的高效厌氧反应器,在处理生活污水中有着巨大的潜力.本研究主要利用宏基因组测序技术对AFMBR系统内的微生物菌群进行探究,结果表明:与接种菌群相比,AFMBR经过一段时间的连续运行后,在属水平上古菌优势菌属由接种时的甲烷囊菌属变为甲烷八叠球菌属、甲烷杆菌属,细菌的整体菌属结构发生了较大变化;在种水平上,系统内不存在较明显的优势菌种.从相对丰度比例≥1%的菌种来看:水解发酵菌群>产氢产乙酸菌群>产甲烷菌群,但各菌群之间相对丰度的差距较小.从基因水平来看,系统内与碳水化合物代谢、氨基酸代谢、能量代谢相关的基因丰度较高,二氧化碳、乙酸转化为甲烷是系统产甲烷的主要途径.
As an efficient anaerobic reactor with low power consumption, anaerobic fluidized bed membrane bioreactor(AFMBR) has great potential in domestic wastewater treatment. In this research, microbes in the AFMBR system were explored by metagenomics technology. The results show that compared with the inoculated bacteria, the archaea were transformed from Methanoculleus to Methanosarcina and Methanobacterium at the genus level. Meanwhile, the structure of bacteria changed enormously. There is no obvious dominant strain in the system at the species level. For relative abundance over 1%, the bacteria population follows in order of hydrolysate fermented bacteria >hydrogen-producing acetogenic bacteria> methanogens, while the difference of their relative abundance was low. From the gene level, the relative abundance of genes related to carbohydrate metabolism, amino acid metabolism and energy metabolism was relatively high. Additionally, the conversion of carbon dioxide and acetic acid is the principal pathway of methane production.
As an efficient anaerobic reactor with low power consumption, anaerobic fluidized bed membrane bioreactor(AFMBR) has great potential in domestic wastewater treatment. In this research, microbes in the AFMBR system were explored by metagenomics technology. The results show that compared with the inoculated bacteria, the archaea were transformed from Methanoculleus to Methanosarcina and Methanobacterium at the genus level. Meanwhile, the structure of bacteria changed enormously. There is no obvious dominant strain in the system at the species level. For relative abundance over 1%, the bacteria population follows in order of hydrolysate fermented bacteria >hydrogen-producing acetogenic bacteria> methanogens, while the difference of their relative abundance was low. From the gene level, the relative abundance of genes related to carbohydrate metabolism, amino acid metabolism and energy metabolism was relatively high. Additionally, the conversion of carbon dioxide and acetic acid is the principal pathway of methane production.
引文
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刘君寒,胡光荣,李福利,等.2011.厌氧消化系统微生物菌群的研究进展[J].工业水处理,31(10):10-14
Magingo F S S,Stumm C K.1991.Nitrogen fixation by Methanobacterium formicicum[J].Fems Microbiology Letters,81(3):273-277
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曲久辉.2014.建设面向未来的中国污水处理概念厂引领城市污水处理高质量发展[J].给水排水,40(3):112-112
Rocheleau S.1999.Differentiation of Methanosaeta concilii and Methanosarcina barkeri in anaerobic mesophilic granular sludge by fluorescent in situ hybridization and confocal scanning laser microscopy[J].Applied & Environmental Microbiology,65(5):2222-2229
Sanz I,Fdz-Polanco F.1989.Anaerobic treatment of municipal sewage in UASB and AFBR reactors[J].Environmental Technology Letters,10(5):453-462
Schauer N L,Ferry J G.1980.Metabolism of formate in Methanobacterium formicicum[J].Journal of Bacteriology,142(3):800
Sun L,Toyonaga M,Ohashi A,et al.2016.Lentimicrobium saccharophilum gen.nov.sp.nov.a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes,and proposal of Lentimicrobiaceae fam.nov[J].International Journal of Systematic and Evolutionary Microbiology,66(7):2635-2642
Takai K.2001.Alkaliphilus transvaalensis gen.nov.sp.nov.an extremely alkaphilic bacterium isolated from a deep South African gold mine[J].International Journal of Systematic and Evolutionary Microbiology,51(4):1245-1256
谭潇,黄靓,杨平,等.2017.盐度对EGSB反应器的运行及厌氧颗粒污泥的影响[J].环境科学,38(8):3422-3428
Tang S,Edwards E A.2013.Complete genome sequence of bacteroidales strain CF from a chloroform-dechlorinating enrichment culture[J].Genome Announcements,1(6),DOI:10.1128/genomeA.01066-13
Tsapekos P,Kougias P G,Vasileiou S A,et al.2017.Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues[J].Bioresource Technology,234:350-359
Walsh C J,Guinane C M,Hill C,et al.2015.In silico identification of bacteriocin gene clusters in the gastrointestinal tract,based on the Human Microbiome Project′s reference genome database[J].Bmc Microbiology,15:183
Yoshida N,Ye L,Baba D,et al.2009.A novel dehalobacter species is involved in extensive 4,5,6,7-tetrachlorophthalide dechlorination[J].Applied and Environmental Microbiology,75(8):2400-2405
Yoshida N,Ye L,Baba D,et al.2009.Reductive dechlorination of polychlorinated biphenyls and dibenzo-p-dioxins in an enrichment culture containing dehalobacter species[J].Microbes & Environments,24(4):343-346
张博康,张岩,陈昌明,等.2018.AFMBR处理低浓度废水的运行特性[J].中国环境科学,38(1):129-135
Zheng H,Dietrich C,Radek R,et al.2016.Endomicrobium proavitum,the first isolate of Endomicrobia class.nov.(phylum Elusimicrobia)-an ultramicrobacterium with an unusual cell cycle that fixes nitrogen with a Group IV nitrogenase[J].Environmental Microbiology,18(1):191-204
Grabowski A,Tindall B J,Bardin V,et al.2005.Petrimonas sulfuriphila gen.nov.sp.nov.a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir[J].International Journal of Systematic and Evolutionary Microbiology,55(3):1113-1121
冯华军,冯小晏,薛飞,等.2011.浙江省典型地区生活污水水质调查研究[J].科技通报,27(3):436-440
Fernandez N,Sierra-Alvarez R,Field J A,et al.2008.Microbial community dynamics in a chemolithotrophic denitrification reactorinoculated with methanogenic granular sludge[J].Chemosphere,70(3):462-474
Ganesan A,Chaussonnerie S,Tarrade A,et al.2008.Cloacibacillus evryensis gen.nov.sp.nov.a novel asaccharolytic,mesophilic,amino-acid-degrading bacterium within the phylum ′Synergistetes′,isolated from an anaerobic sludge digester[J].International Journal of Systematic & Evolutionary Microbiology,58(9):2003-2012
Fang H H P,Shi X,Zhang T.2006.Effect of activated carbon on fouling of activated sludge filtration[J].Desalination,189(1/3):193-199
Kadnikov V V,Mardanov A V,Podosokorskaya O A,et al.2013.Genomic analysis of melioribacter roseus,facultatively anaerobic organotrophic bacterium representing a novel deep lineage within bacteriodetes/chlorobi group[J].PLoS One,8(1):e53047
Kato S,Kosaka T,Watanabe K.2010.Substrate-dependent transcriptomic shifts in Pelotomaculum thermopropionicum grown in syntrophic co-culture with Methanothermobacter thermautotrophicus[J].Microbial Biotechnology,2(5):575-584
Kim J,Kim K,Ye H,et al.2011.Anaerobic fluidized bed membrane bioreactor for wastewater treatment[J].Environmental Science & Technology,45(2):576-581
Kumar A G,Kirubagaran R,Nagesh N,et al.2010.Influence of fermentation metabolites on redox potential in anaerobic digestion of proteinaceous solid wastes by Synergistes sp.[J].Engineering in Life Sciences,10(4):368-373
Leng L,Yang P,Singh S,et al.2017.A review on the bioenergetics of anaerobic microbial metabolism close to the thermodynamic limits and its implications for digestion applications[J].Bioresource Technology,247:1095-1106
Li Z,Yoshida N,Wang A,et al.2015.Anaerobic mineralization of 2,4,6-tribromophenol to CO2 by a synthetic microbial community comprising Clostridium,Dehalobacter,and Desulfatiglans[J].Bioresource Technology,176:225-232
Lin Q,Fang X,Ho A,et al.2017.Different substrate regimes determine transcriptional profiles and gene co-expression in Methanosarcina barkeri (DSM 800)[J].Applied Microbiology and Biotechnology,101(19):7303-7316
刘君寒,胡光荣,李福利,等.2011.厌氧消化系统微生物菌群的研究进展[J].工业水处理,31(10):10-14
Magingo F S S,Stumm C K.1991.Nitrogen fixation by Methanobacterium formicicum[J].Fems Microbiology Letters,81(3):273-277
Mccarty P L.1981.One hundred years of anaerobic treatment[C]//Anaerobic Digestion:Second International Symposium on Anaerobic Digestion.Travemhunde
Mcinerney M J.1981.Syntrophomonas wolfei gen.nov.sp.nov.an anaerobic,syntrophic,fatty-acid oxidizing bacterium[J].Applied & Environmental Microbiology,41(4):1029-1039
Bérubé P R,Hall E R,Sutton P M.2006.Parameters governing permeate flux in an anaerobic membrane bioreactor treating low-strength municipal wastewaters:a literature review[J].Water Environment Research,78(8):887-896
Qiu Y L,Hanada S,Ohashi A,et al.2008.Syntrophorhabdus aromaticivorans gen.nov.sp.nov.the first cultured anaerobe capable of degrading phenol to acetate in obligate syntrophic associations with a hydrogenotrophic methanogen[J].Applied and Environmental Microbiology,74(7):2051-2058
曲久辉.2014.建设面向未来的中国污水处理概念厂引领城市污水处理高质量发展[J].给水排水,40(3):112-112
Rocheleau S.1999.Differentiation of Methanosaeta concilii and Methanosarcina barkeri in anaerobic mesophilic granular sludge by fluorescent in situ hybridization and confocal scanning laser microscopy[J].Applied & Environmental Microbiology,65(5):2222-2229
Sanz I,Fdz-Polanco F.1989.Anaerobic treatment of municipal sewage in UASB and AFBR reactors[J].Environmental Technology Letters,10(5):453-462
Schauer N L,Ferry J G.1980.Metabolism of formate in Methanobacterium formicicum[J].Journal of Bacteriology,142(3):800
Sun L,Toyonaga M,Ohashi A,et al.2016.Lentimicrobium saccharophilum gen.nov.sp.nov.a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes,and proposal of Lentimicrobiaceae fam.nov[J].International Journal of Systematic and Evolutionary Microbiology,66(7):2635-2642
Takai K.2001.Alkaliphilus transvaalensis gen.nov.sp.nov.an extremely alkaphilic bacterium isolated from a deep South African gold mine[J].International Journal of Systematic and Evolutionary Microbiology,51(4):1245-1256
谭潇,黄靓,杨平,等.2017.盐度对EGSB反应器的运行及厌氧颗粒污泥的影响[J].环境科学,38(8):3422-3428
Tang S,Edwards E A.2013.Complete genome sequence of bacteroidales strain CF from a chloroform-dechlorinating enrichment culture[J].Genome Announcements,1(6),DOI:10.1128/genomeA.01066-13
Tsapekos P,Kougias P G,Vasileiou S A,et al.2017.Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues[J].Bioresource Technology,234:350-359
Walsh C J,Guinane C M,Hill C,et al.2015.In silico identification of bacteriocin gene clusters in the gastrointestinal tract,based on the Human Microbiome Project′s reference genome database[J].Bmc Microbiology,15:183
Yoshida N,Ye L,Baba D,et al.2009.A novel dehalobacter species is involved in extensive 4,5,6,7-tetrachlorophthalide dechlorination[J].Applied and Environmental Microbiology,75(8):2400-2405
Yoshida N,Ye L,Baba D,et al.2009.Reductive dechlorination of polychlorinated biphenyls and dibenzo-p-dioxins in an enrichment culture containing dehalobacter species[J].Microbes & Environments,24(4):343-346
张博康,张岩,陈昌明,等.2018.AFMBR处理低浓度废水的运行特性[J].中国环境科学,38(1):129-135
Zheng H,Dietrich C,Radek R,et al.2016.Endomicrobium proavitum,the first isolate of Endomicrobia class.nov.(phylum Elusimicrobia)-an ultramicrobacterium with an unusual cell cycle that fixes nitrogen with a Group IV nitrogenase[J].Environmental Microbiology,18(1):191-204
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