大鳞副泥鳅5个野生群体的遗传多样性分析
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摘要
利用微卫星分子标记分析了天津地区于桥水库、大黄堡湿地、七里海湿地、团泊水库和潮白新河5个野生大鳞副泥鳅群体的遗传多样性。5个群体在12个微卫星位点共检测到98个等位基因,平均等位基因数为8.500;有效等位基因数为2.713;平均观测杂合度为0.526;平均期望杂合度为0.544。5个群体间的遗传分化指数介于0.021～0.106之间,平均遗传分化指数为0.059,属中低水平的遗传分化。AMOVA分析结果显示,在总的变异中,94.1%的遗传变异来自群体内,5.90%的遗传变异来自于群体间。根据Nei's遗传距离所绘制的系统树显示,于桥水库、团泊水库、七里海湿地和大黄堡湿地4个群体的遗传距离相对较小聚为一支,潮白新河群体单独为一支。遗传瓶颈效应分析表明,该5个群体近期未经受遗传瓶颈效应,处于突变-漂移平衡。总体来看,天津地区5个野生大鳞副泥鳅群体的遗传多样性较高,可作为品种选育的基础群体。
The genetic diversity of five wild Paramisgurnus dabryanus populations from Yuqiao Reservoir,Dahuangpu Wetland, Qilihai Wetland, Tuanbo Reservoir and New Chaobai River in Tianjin were analyzed using microsatellite markers. A total of 98 alleles were detected in the five populations. The average number of alleles was 8.500, the number of effective alleles was 2.713, the average values of observed heterozygosity was 0.526, and the average values of expected heterozygosity was 0.544. Genetic differentiation indexes( Fst) were between0.021～0.106, and the average Fst value was 0.059, which indicated that the genetic differentiation were at a medium low level. AMOVA analysis showed that the genetic variation was 94.1% within populations while it was 5.90% among populations. The results of the dendrogram based on Nei's genetic distance showed that Yuqiao Reservoir, Tuanbo Reservoir, Dahuangpu Wetland and Qilihai Wetland populations were clustered into one clade due to relatively closer genetic distances, while New Chaobai River population was separated into another group.The genetic bottleneck analysis revealed that the five populations didn't experience a bottleneck effect in recent years and were in mutation-drift equilibrium. On the whole, the genetic diversity of the five wild P. dabryanus populations in Tianjin is relatively high,which can be used as the base group for breeding.
The genetic diversity of five wild Paramisgurnus dabryanus populations from Yuqiao Reservoir,Dahuangpu Wetland, Qilihai Wetland, Tuanbo Reservoir and New Chaobai River in Tianjin were analyzed using microsatellite markers. A total of 98 alleles were detected in the five populations. The average number of alleles was 8.500, the number of effective alleles was 2.713, the average values of observed heterozygosity was 0.526, and the average values of expected heterozygosity was 0.544. Genetic differentiation indexes( Fst) were between0.021～0.106, and the average Fst value was 0.059, which indicated that the genetic differentiation were at a medium low level. AMOVA analysis showed that the genetic variation was 94.1% within populations while it was 5.90% among populations. The results of the dendrogram based on Nei's genetic distance showed that Yuqiao Reservoir, Tuanbo Reservoir, Dahuangpu Wetland and Qilihai Wetland populations were clustered into one clade due to relatively closer genetic distances, while New Chaobai River population was separated into another group.The genetic bottleneck analysis revealed that the five populations didn't experience a bottleneck effect in recent years and were in mutation-drift equilibrium. On the whole, the genetic diversity of the five wild P. dabryanus populations in Tianjin is relatively high,which can be used as the base group for breeding.
引文
Bai X.H.,2014,Microsatellites-assisted breeding and estimation of genetic parameters for growth-related traits of dojo loach Misgurnus anguillicaudatus,Dissertation for Ph.D.,Huazhong Agricultural University,Supervisor:Wang W.M.,pp.30(白晓慧,2014,微卫星标记辅助泥鳅生长性状选育及其遗传参数估计,博士学位论文,华中农业大学,导师:王卫民,pp.30)
Balloux F.,and Lugon-Moulin N.,2002,The estimation of population differentiation with microsatellite markers,Molecular Ecology,11(2):155-165
Cornuet J.M.,and Luikart G.,1996,Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data,Genetics,144(4):2001-2014
DeWoody J.A.,and Avise J.C.,2000,Microsatellite variation in marine,freshwater and anadromous fishes compared with other animals,Journal of Fish Biology,56(3):461-473
Excoffier L.,and Lischer H.E.L.,2010,Arlequin suite ver 3.5:Anew series of programs to perform population genetics analyses under Linux and Windows,Molecular Ecology Resources,10(3):564-567
Hou N.,Zhang Y.,Lu C.Y.,Li Y.,Li D.Y.,Ji X.,Ding L.,and Sun X.W.,2007,Genetic potential analysis of Germany mirror carp(Cyprinus carpio L.)using microsatellite markers,Yichuan(Hereditas),29(12):1509-1518(侯宁,张研,鲁翠云,李勇,李大宇,季旭,丁雷,孙效文,2007,微卫星DNA标记分析德国镜鲤的遗传潜力,遗传,29(12):1509-1518)
Kalinowski S.T.,Taper M.L.,and Marshall T.C.,2007,Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment,Molecular Ecology,16(5):1099-1106
Li C.J.,Ling Q.F.,Ge C.,Zhu P.F.,Wang G.C.,Ye Z.Q.,and Han X.F.,2014,Genetic diversity of Paramisguruus dabryauus from four big lakes in China,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),30(5):1087-1094(李彩娟,凌去非,葛辰,朱鹏飞,王国成,叶竹青,韩晓飞,2014,中国4大湖泊大鳞副泥鳅群体遗传多样性分析,江苏农业学报,30(5):1087-1094)
Li M.D.,ed.,2011,The fauna of fishes in Tianjin,Tianjin Science and Technology Press,Tianjin,China,pp.85(李明德,主编,2011,天津鱼类志,天津科学技术出版社,中国,天津,pp.85)
Li Y.J.,Zhang B.,Xu B.L.,Li J.L.,and Ma Y.,2017,Preliminary research on rice order planting in Tianjin area,Tianjin Nongye Kexue(Tianjin Agricultural Sciences),23(1):65-67(李永杰,张蓓,徐宝梁,李景龙,马煜,2017,水稻订单种植在天津地区的初步探究,天津农业科学,23(1):65-67)
Moradi A.,and Keyvanshokooh S.,2013,Microsatellite DNAmarker in aquatic organisms,Sci.J.Biol.Sci.,2(9):184-189
Peng S.T.,Zhao Y.D.,Zhang G.Y.,Jing L.,Zhan S.F.,and Yu H.,2009,Analysis on current situation of biological diversity and protection measures in Qilihai Wetland,Shuidao Gangkou(Journal of Waterway and Harbor),30(2):135-138(彭士涛,赵益栋,张光玉,井亮,詹水芬,于航,2009,七里海湿地生物多样性现状及其保护对策,水道港口,30(2):135-138)
Rousset F.,2008,Genepop'007:a complete reimplementation of the Genepop software for Windows and Linux,Molecular E-cology Resources,8(1):103-106
Spencer C.C.,Neigel J.E.,and Leberg P.L.,2000,Experimental evaluation of the usefulness of microsatellite DNA for detecting demographic bottlenecks,Molecular Ecology,9(10):1517-1528
Takezaki N.,and Nei M.,1996,Genetic distances and recon struction of phylogenetic trees from microsatellite DNA,Genetics,144(1):389-399
Wang L.,Zhang L.D.,Wan Y.M.,Tan Z.J.,and Sun X.W.,2010,Isolation of microsatellite markers from Paralichthys olivaceus and its application in genetic structure analysis,Yichuan(Hereditas),32(10):1057-1064(王蕾,张立冬,万玉美,谭照君,孙效文,2010,牙鲆微卫星标记的筛选及群体遗传结构分析,遗传,32(10):1057-1064)
Yang Z.Y.,Miao Y.W.,Li D.L.,Huo J.L.,Chen T.,He C.Y.,Chuang X.H,and Tang S.K.,2007,Unfolding of population structure in Dehong Buffalo using microsatellite DNA markers,Dongwuxue Yanjiu(Zoological Research),28(6):659-663(杨泽宇,苗永旺,李大林,霍金龙,陈涛,何朝阳,创向辉,汤守锟,2007,德宏水牛微卫星标记分析的群体遗传变异,动物学研究,28(6):659-663)
Ye Z.Q.,Ling Q.F.,Li C.J.,and Han X.F.,2015,Genetic Structure analysis of five fam ilies of Paramisgurnus dabryanus,Shuishengtai Xue Zazhi(Journal of Hydroecology),36(1):66-73(叶竹青,凌去,李彩娟,韩晓飞,2015,5个大鳞副泥鳅家系的遗传结构分析,水生态学杂志,36(1):66-73)
Yeh F.C.,Yang R.C.,and Boyle T.,1999,POPGENE version l.31,Microsoft window-based freeware for population genetic analysis,University of Alberta and Centre for International Forestry Research,Edmonton,pp.1-29
You C.H.,Tong J.G.,and Yu X.M.,2012,Microsatellite DNAanalysis on genetic diversity of seven populations of Paramisgurnus dabryanus,Shuishengtai Xue Zazhi(Journal of Hydroecology),33(1):84-91(游翠红,童金苟,俞小牧,2012,大鳞副泥鳅7个群体遗传变异的微卫星分析,水生态学杂志,33(1):84-91)
Zhang Z.Y.,Li L.,Sun Y.Q.,and Guo J.,2014,Problems and protection suggestions of Tianjin wetlands,Nongye Zaihai Yanjiu(Journal of Agricultural Catastrophology),4(12):58-60(张征云,李莉,孙艳青,郭健,2014,天津湿地存在的主要问题与保护对策建议,农业灾害研究,4(12):58-60)
Balloux F.,and Lugon-Moulin N.,2002,The estimation of population differentiation with microsatellite markers,Molecular Ecology,11(2):155-165
Cornuet J.M.,and Luikart G.,1996,Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data,Genetics,144(4):2001-2014
DeWoody J.A.,and Avise J.C.,2000,Microsatellite variation in marine,freshwater and anadromous fishes compared with other animals,Journal of Fish Biology,56(3):461-473
Excoffier L.,and Lischer H.E.L.,2010,Arlequin suite ver 3.5:Anew series of programs to perform population genetics analyses under Linux and Windows,Molecular Ecology Resources,10(3):564-567
Hou N.,Zhang Y.,Lu C.Y.,Li Y.,Li D.Y.,Ji X.,Ding L.,and Sun X.W.,2007,Genetic potential analysis of Germany mirror carp(Cyprinus carpio L.)using microsatellite markers,Yichuan(Hereditas),29(12):1509-1518(侯宁,张研,鲁翠云,李勇,李大宇,季旭,丁雷,孙效文,2007,微卫星DNA标记分析德国镜鲤的遗传潜力,遗传,29(12):1509-1518)
Kalinowski S.T.,Taper M.L.,and Marshall T.C.,2007,Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment,Molecular Ecology,16(5):1099-1106
Li C.J.,Ling Q.F.,Ge C.,Zhu P.F.,Wang G.C.,Ye Z.Q.,and Han X.F.,2014,Genetic diversity of Paramisguruus dabryauus from four big lakes in China,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),30(5):1087-1094(李彩娟,凌去非,葛辰,朱鹏飞,王国成,叶竹青,韩晓飞,2014,中国4大湖泊大鳞副泥鳅群体遗传多样性分析,江苏农业学报,30(5):1087-1094)
Li M.D.,ed.,2011,The fauna of fishes in Tianjin,Tianjin Science and Technology Press,Tianjin,China,pp.85(李明德,主编,2011,天津鱼类志,天津科学技术出版社,中国,天津,pp.85)
Li Y.J.,Zhang B.,Xu B.L.,Li J.L.,and Ma Y.,2017,Preliminary research on rice order planting in Tianjin area,Tianjin Nongye Kexue(Tianjin Agricultural Sciences),23(1):65-67(李永杰,张蓓,徐宝梁,李景龙,马煜,2017,水稻订单种植在天津地区的初步探究,天津农业科学,23(1):65-67)
Moradi A.,and Keyvanshokooh S.,2013,Microsatellite DNAmarker in aquatic organisms,Sci.J.Biol.Sci.,2(9):184-189
Peng S.T.,Zhao Y.D.,Zhang G.Y.,Jing L.,Zhan S.F.,and Yu H.,2009,Analysis on current situation of biological diversity and protection measures in Qilihai Wetland,Shuidao Gangkou(Journal of Waterway and Harbor),30(2):135-138(彭士涛,赵益栋,张光玉,井亮,詹水芬,于航,2009,七里海湿地生物多样性现状及其保护对策,水道港口,30(2):135-138)
Rousset F.,2008,Genepop'007:a complete reimplementation of the Genepop software for Windows and Linux,Molecular E-cology Resources,8(1):103-106
Spencer C.C.,Neigel J.E.,and Leberg P.L.,2000,Experimental evaluation of the usefulness of microsatellite DNA for detecting demographic bottlenecks,Molecular Ecology,9(10):1517-1528
Takezaki N.,and Nei M.,1996,Genetic distances and recon struction of phylogenetic trees from microsatellite DNA,Genetics,144(1):389-399
Wang L.,Zhang L.D.,Wan Y.M.,Tan Z.J.,and Sun X.W.,2010,Isolation of microsatellite markers from Paralichthys olivaceus and its application in genetic structure analysis,Yichuan(Hereditas),32(10):1057-1064(王蕾,张立冬,万玉美,谭照君,孙效文,2010,牙鲆微卫星标记的筛选及群体遗传结构分析,遗传,32(10):1057-1064)
Yang Z.Y.,Miao Y.W.,Li D.L.,Huo J.L.,Chen T.,He C.Y.,Chuang X.H,and Tang S.K.,2007,Unfolding of population structure in Dehong Buffalo using microsatellite DNA markers,Dongwuxue Yanjiu(Zoological Research),28(6):659-663(杨泽宇,苗永旺,李大林,霍金龙,陈涛,何朝阳,创向辉,汤守锟,2007,德宏水牛微卫星标记分析的群体遗传变异,动物学研究,28(6):659-663)
Ye Z.Q.,Ling Q.F.,Li C.J.,and Han X.F.,2015,Genetic Structure analysis of five fam ilies of Paramisgurnus dabryanus,Shuishengtai Xue Zazhi(Journal of Hydroecology),36(1):66-73(叶竹青,凌去,李彩娟,韩晓飞,2015,5个大鳞副泥鳅家系的遗传结构分析,水生态学杂志,36(1):66-73)
Yeh F.C.,Yang R.C.,and Boyle T.,1999,POPGENE version l.31,Microsoft window-based freeware for population genetic analysis,University of Alberta and Centre for International Forestry Research,Edmonton,pp.1-29
You C.H.,Tong J.G.,and Yu X.M.,2012,Microsatellite DNAanalysis on genetic diversity of seven populations of Paramisgurnus dabryanus,Shuishengtai Xue Zazhi(Journal of Hydroecology),33(1):84-91(游翠红,童金苟,俞小牧,2012,大鳞副泥鳅7个群体遗传变异的微卫星分析,水生态学杂志,33(1):84-91)
Zhang Z.Y.,Li L.,Sun Y.Q.,and Guo J.,2014,Problems and protection suggestions of Tianjin wetlands,Nongye Zaihai Yanjiu(Journal of Agricultural Catastrophology),4(12):58-60(张征云,李莉,孙艳青,郭健,2014,天津湿地存在的主要问题与保护对策建议,农业灾害研究,4(12):58-60)
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