诺氟沙星对生物转盘反应器处理污染河水性能的影响
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摘要
诺氟沙星作为一种常用的氟喹诺酮类抗生素,普遍存在于自然水体中,其输入会刺激水环境进而影响受污染水体的处理效果.为了探究诺氟沙星对反应器处理污染河水性能的影响,向反应器中投加不同浓度的诺氟沙星,研究反应器中NH~+_4-N、TN、TP和COD_(Mn)去除率及生物膜特性的变化.结果表明,不同浓度的诺氟沙星对反应器中污染物去除率的影响不同.诺氟沙星浓度为0.05μg·L~(-1)时,对NH~+_4-N、TN去除率的影响表现为先促进后抑制;随着诺氟沙星浓度的持续增加,对NH~+_4-N、TN去除率的影响更加显著(p<0.05).反应器TP的去除率随诺氟沙星添加浓度的不断增加而明显降低,且添加浓度越高下降趋势越明显(p<0.05).而诺氟沙星的存在对COD_(Mn)去除没有明显作用(p>0.05).诺氟沙星的添加使生物膜VSS/TSS、TTC-脱氢酶活性降低,同时,微生物为进行自我保护分泌了大量EPS从而增加了蛋白和多糖含量.诺氟沙星的存在改变了生物膜EPS化学组分及化学含量,并且降低了生物膜物种多样性和丰富度.
As a commonly used fluoroquinolone antibiotic, norfloxacin is ubiquitous in natural water, which could influence the treatment of contaminated water. In order to investigate how norfloxacin take effect on the treatment of polluted river water, different concentrations of norfloxacin were added to the reactor. The results show that different concentrations of norfloxacin had various effects on the removal rates of contaminants. When the concentration of norfloxacin was 0.05 μg·L~(-1), the removal rates of NH~+_4-N and TN were first promoted and then inhibited. With increasing norfloxacin concentrations, the removal rates of NH~+_4-N and TN were significantly affected(p<0.05). The removal rates of TP in the reactor decreased significantly with the increasing of norfloxacin concentrations. Norfloxacin had no significant effects on COD_(Mn) removal(p>0.05). The addition of norfloxacin reduced the VSS/TSS and TTC-dehydrogenase activity of biofilm, indicating that the microorganisms secreted a large amount of EPS for self-protection to increase the proteoglycan content. Norfloxacin changed the chemical composition and content of EPS and reduced the diversity and richness of biofilm species.
As a commonly used fluoroquinolone antibiotic, norfloxacin is ubiquitous in natural water, which could influence the treatment of contaminated water. In order to investigate how norfloxacin take effect on the treatment of polluted river water, different concentrations of norfloxacin were added to the reactor. The results show that different concentrations of norfloxacin had various effects on the removal rates of contaminants. When the concentration of norfloxacin was 0.05 μg·L~(-1), the removal rates of NH~+_4-N and TN were first promoted and then inhibited. With increasing norfloxacin concentrations, the removal rates of NH~+_4-N and TN were significantly affected(p<0.05). The removal rates of TP in the reactor decreased significantly with the increasing of norfloxacin concentrations. Norfloxacin had no significant effects on COD_(Mn) removal(p>0.05). The addition of norfloxacin reduced the VSS/TSS and TTC-dehydrogenase activity of biofilm, indicating that the microorganisms secreted a large amount of EPS for self-protection to increase the proteoglycan content. Norfloxacin changed the chemical composition and content of EPS and reduced the diversity and richness of biofilm species.
引文
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Chao Y Q,Gao G H,Yang T T,et al.2015.Effects of fluoroquinolone antibiotics on reactor performance and microbial community structure of a membrane bioreactor[J].Chemical Engineering Journal,280:448-458
Fr-Olund B,Griebe T,Nielsen P H.1995.Enzymatic activity in the activated-sludge floc matrix[J].Applied Microbiology and Biotechnology,43(4):755-761
高立红,史亚利,厉文辉,等.2013.抗生素环境行为及其环境效应研究进展[J].环境化学,9(9):1619-1633
国家环境保护总局.2002.水和废水监测分析方法(第四版)[M].北京:中国环境科学出版社
He Q L,Zhou J,Wang H Y,et al.2016.Microbial population dynamics during sludge granulation in an A/O/A sequencing batch reactor[J].Bioresource Technology:Biomass,Bioenergy,Biowastes,Conversion Technologies,Biotransformations,Production Technologies,214:1-8
Oosterhof J J H,Elving G J,Stokroos I et al.2003.The influence of antimicrobial peptides and mucolytics on the integrity of biofilms consisting of bacteria and yeasts as affecting voice prosthetic air flow resistances[J].Biofouling,19(6):347-353
柯润辉,蒋愉林,黄清辉,等.2014.上海某城市污水处理厂污水中药物类个人护理用品PPCPs的调查研究[J].生态毒理学报,9(6):1146-1155
Kong Q,He X,Ma S S,et al.2017.The performance and evolution of bacterial community of activated sludge exposed to trimethoprim in a sequencing batch reactor[J].Bioresource Technology:Biomass,Bioenergy,Biowastes,Conversion Technologies,Biotransformations,Production Technologies,244(1):872-879
郎印海,刘犇,何淑雯,等.2018.诺氟沙星在壳聚糖-生物炭复合材料上的解吸行为研究[J].中国海洋大学学报(自然科学版),48(11):19-25
李华瑞,梁立锐,程士,等.2015.水中诺氟沙星污染现状及其处理方法研究进展[J].山东化工,44(15):60-62
李一兵,董俊良,李静,等.2017.A2/O工艺法深化处理混合污水的试验研究[J].河北工业大学学报,(3):100-104
Liu Y.1997.Estimating minimum fixed biomass concentration and active thickness of nitrifying biofilm[J].Journal of Environmental Engineering,123(2):198-202
娄高彬,刘金泉,赵淑萍,等.2015.低温条件下生物转盘处理城镇污水的研究[J].中国给水排水,31(23):101-103
Ludwig T G,Goldberg J V.1956.The anthrone method for the determination of carbohydrates in foods and in oral rinsing[J].Journal of Dental Research,35(35):90-94
Luo Y,Xu L,Rysz M.2011.Occurrence and transport of tetracycline,sulfonamide,quinolone,and macrolide antibiotics in the Haihe River Basin,China[J].Environmental Science & Technology,45(5):1827-1833
Nielsen P H,Jahn A,Palmgren R.1997.Conceptual model for production and composition of exopolymers in biofilms[J].Water Science and Technology,36(1):11-19
Oosterhof J J,Elving G J,Stokroos I,et al.2003.The influence of antimicrobial peptides and mucolytics on the integrity of biofilms consisting of bacteria and yeasts as affecting voice prosthetic air flow resistances[J].Biofouling,19(6):347-353
Qiao M,Ying G G,Singer A C,et al.2018.Review of antibiotic resis-tance in China and its environment[J].Environment International,110:160-172
Ramesh A,Lee D J,Hong S G.2006.Soluble microbial products(SMP) and soluble extracellular polymeric substances(EPS) from wastewater sludge[J].Applied Microbiology and Biotechnology,73(1):219-225
Sheng G P,Yu H Q.2006.Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Water Research,40:1233-1239
宋冬,刘珊,何义亮,等.2015.城市污水处理厂含铝污泥回流除磷分析[J].应用化工,44(12):2301-2304
王文东,刘荟,马翠,等.2016.一体式生物净化-沉淀池对微污染水体污染物的强化去除性能[J].环境科学,37(10):3858-3863
Wang Z,Gao M,Wei J,et al.2016.Long-term effects of salinity on extracellular polymeric substances,microbial activity and microbial community from biofilm and suspended sludge in an anoxic-aerobic sequencing batch biofilm reactor[J].Journal of the Taiwan Institute of Chemical Engineers,68:275-280
魏东斌,肖铭,阴俊霞,等.2011.色谱及联用技术在药物污染检测中的应用[J].环境化学,30(1):263-271
Xia S,Li J,Wang R.2008.Nitrogen removal performance and microbial community structure dynamics response to carbon nitrogen ratio in a compact suspended carrier biofilm reactor[J].Ecological engineering:The Journal of Ecotechnology,32(3):256-262
魏东洋,董磐磐,李杰,等.2013.新型载体生物转盘处理微污染河水效果研究[J].中国环境科学,33(s1):156-159
许雯佳,成小英.2018.水力停留时间对活性炭生物转盘处理污染河水的影响[J].环境科学,39(1):202-211
杨常青,王龙星,侯晓虹,等.2012.大辽河水系河水中16种抗生素的污染水平分析[J].色谱,30(8):756-762
杨春雪.2015.嗜热菌强化剩余污泥水解及短链脂肪酸积累规律研究[D].哈尔滨:哈尔滨工业大学
Yang S Y,Guo L,She Z L,et al.2016.Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress[J].Bioprocess and Biosystems Engineering,39(9):1375-1389
杨涛,郝学凯,陈宝玉,等.2018.Al3+对序批式生物膜反应器(SBBR)中污泥脱氢酶活性(DHA)和胞外聚合物(EPS)的影响[J].环境科学学报,38(4):1453-1459
Yang Y,Li B,Ju F,et al.2013.Exploring variation of antibiotic resis-tance genes in activated sludge over a four-year period through a metagenomic approach[J].Environmental Science & Technology,47(18):10197-10205
张涵瑜,王兆炜,高俊红,等.2016.芦苇基和污泥基生物炭对水体中诺氟沙星的吸附性能[J].环境科学,37(2):689-696
张金莲,贺锋,梁威,等.2008.Zn2+,Co2+和Mn2+对人工湿地基质生物膜的影响[J].中国环境科学,28(2):158-162
Zhang Q Q,Ying G G,Pan C G,et al.2015.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:Source analysis,multimedia modeling,and linkage to bacterial resistance[J].Environmental Science & Technology,49(11):6772-6782
朱洁,东刘成,陈洪斌,等.2012.不同填料类型污染源水生物预处理的比较[J].中国环境科学,32(3):447-453
邹高龙,刘志文,董洁平,等.环丙沙星在污水处理过程中的迁移转化及对污水生物处理的影响[J].环境科学学报,2019(2)
章洪涛.2015.重金属与抗生素对SBBR处理猪场废水的影响[D].南昌:南昌大学
邹世春,朱春敬,贺竹梅,等.2009.北江河水中抗生素抗性基因污染初步研究[J].生态毒理学报,4(5):655-660
Zumft W G.1997.Cell biology and molecular basis of denitrification[J].Microbiology and Molecular Biology Reviews,61(4):533-616
Chao Y Q,Gao G H,Yang T T,et al.2015.Effects of fluoroquinolone antibiotics on reactor performance and microbial community structure of a membrane bioreactor[J].Chemical Engineering Journal,280:448-458
Fr-Olund B,Griebe T,Nielsen P H.1995.Enzymatic activity in the activated-sludge floc matrix[J].Applied Microbiology and Biotechnology,43(4):755-761
高立红,史亚利,厉文辉,等.2013.抗生素环境行为及其环境效应研究进展[J].环境化学,9(9):1619-1633
国家环境保护总局.2002.水和废水监测分析方法(第四版)[M].北京:中国环境科学出版社
He Q L,Zhou J,Wang H Y,et al.2016.Microbial population dynamics during sludge granulation in an A/O/A sequencing batch reactor[J].Bioresource Technology:Biomass,Bioenergy,Biowastes,Conversion Technologies,Biotransformations,Production Technologies,214:1-8
Oosterhof J J H,Elving G J,Stokroos I et al.2003.The influence of antimicrobial peptides and mucolytics on the integrity of biofilms consisting of bacteria and yeasts as affecting voice prosthetic air flow resistances[J].Biofouling,19(6):347-353
柯润辉,蒋愉林,黄清辉,等.2014.上海某城市污水处理厂污水中药物类个人护理用品PPCPs的调查研究[J].生态毒理学报,9(6):1146-1155
Kong Q,He X,Ma S S,et al.2017.The performance and evolution of bacterial community of activated sludge exposed to trimethoprim in a sequencing batch reactor[J].Bioresource Technology:Biomass,Bioenergy,Biowastes,Conversion Technologies,Biotransformations,Production Technologies,244(1):872-879
郎印海,刘犇,何淑雯,等.2018.诺氟沙星在壳聚糖-生物炭复合材料上的解吸行为研究[J].中国海洋大学学报(自然科学版),48(11):19-25
李华瑞,梁立锐,程士,等.2015.水中诺氟沙星污染现状及其处理方法研究进展[J].山东化工,44(15):60-62
李一兵,董俊良,李静,等.2017.A2/O工艺法深化处理混合污水的试验研究[J].河北工业大学学报,(3):100-104
Liu Y.1997.Estimating minimum fixed biomass concentration and active thickness of nitrifying biofilm[J].Journal of Environmental Engineering,123(2):198-202
娄高彬,刘金泉,赵淑萍,等.2015.低温条件下生物转盘处理城镇污水的研究[J].中国给水排水,31(23):101-103
Ludwig T G,Goldberg J V.1956.The anthrone method for the determination of carbohydrates in foods and in oral rinsing[J].Journal of Dental Research,35(35):90-94
Luo Y,Xu L,Rysz M.2011.Occurrence and transport of tetracycline,sulfonamide,quinolone,and macrolide antibiotics in the Haihe River Basin,China[J].Environmental Science & Technology,45(5):1827-1833
Nielsen P H,Jahn A,Palmgren R.1997.Conceptual model for production and composition of exopolymers in biofilms[J].Water Science and Technology,36(1):11-19
Oosterhof J J,Elving G J,Stokroos I,et al.2003.The influence of antimicrobial peptides and mucolytics on the integrity of biofilms consisting of bacteria and yeasts as affecting voice prosthetic air flow resistances[J].Biofouling,19(6):347-353
Qiao M,Ying G G,Singer A C,et al.2018.Review of antibiotic resis-tance in China and its environment[J].Environment International,110:160-172
Ramesh A,Lee D J,Hong S G.2006.Soluble microbial products(SMP) and soluble extracellular polymeric substances(EPS) from wastewater sludge[J].Applied Microbiology and Biotechnology,73(1):219-225
Sheng G P,Yu H Q.2006.Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Water Research,40:1233-1239
宋冬,刘珊,何义亮,等.2015.城市污水处理厂含铝污泥回流除磷分析[J].应用化工,44(12):2301-2304
王文东,刘荟,马翠,等.2016.一体式生物净化-沉淀池对微污染水体污染物的强化去除性能[J].环境科学,37(10):3858-3863
Wang Z,Gao M,Wei J,et al.2016.Long-term effects of salinity on extracellular polymeric substances,microbial activity and microbial community from biofilm and suspended sludge in an anoxic-aerobic sequencing batch biofilm reactor[J].Journal of the Taiwan Institute of Chemical Engineers,68:275-280
魏东斌,肖铭,阴俊霞,等.2011.色谱及联用技术在药物污染检测中的应用[J].环境化学,30(1):263-271
Xia S,Li J,Wang R.2008.Nitrogen removal performance and microbial community structure dynamics response to carbon nitrogen ratio in a compact suspended carrier biofilm reactor[J].Ecological engineering:The Journal of Ecotechnology,32(3):256-262
魏东洋,董磐磐,李杰,等.2013.新型载体生物转盘处理微污染河水效果研究[J].中国环境科学,33(s1):156-159
许雯佳,成小英.2018.水力停留时间对活性炭生物转盘处理污染河水的影响[J].环境科学,39(1):202-211
杨常青,王龙星,侯晓虹,等.2012.大辽河水系河水中16种抗生素的污染水平分析[J].色谱,30(8):756-762
杨春雪.2015.嗜热菌强化剩余污泥水解及短链脂肪酸积累规律研究[D].哈尔滨:哈尔滨工业大学
Yang S Y,Guo L,She Z L,et al.2016.Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress[J].Bioprocess and Biosystems Engineering,39(9):1375-1389
杨涛,郝学凯,陈宝玉,等.2018.Al3+对序批式生物膜反应器(SBBR)中污泥脱氢酶活性(DHA)和胞外聚合物(EPS)的影响[J].环境科学学报,38(4):1453-1459
Yang Y,Li B,Ju F,et al.2013.Exploring variation of antibiotic resis-tance genes in activated sludge over a four-year period through a metagenomic approach[J].Environmental Science & Technology,47(18):10197-10205
张涵瑜,王兆炜,高俊红,等.2016.芦苇基和污泥基生物炭对水体中诺氟沙星的吸附性能[J].环境科学,37(2):689-696
张金莲,贺锋,梁威,等.2008.Zn2+,Co2+和Mn2+对人工湿地基质生物膜的影响[J].中国环境科学,28(2):158-162
Zhang Q Q,Ying G G,Pan C G,et al.2015.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:Source analysis,multimedia modeling,and linkage to bacterial resistance[J].Environmental Science & Technology,49(11):6772-6782
朱洁,东刘成,陈洪斌,等.2012.不同填料类型污染源水生物预处理的比较[J].中国环境科学,32(3):447-453
邹高龙,刘志文,董洁平,等.环丙沙星在污水处理过程中的迁移转化及对污水生物处理的影响[J].环境科学学报,2019(2)
章洪涛.2015.重金属与抗生素对SBBR处理猪场废水的影响[D].南昌:南昌大学
邹世春,朱春敬,贺竹梅,等.2009.北江河水中抗生素抗性基因污染初步研究[J].生态毒理学报,4(5):655-660
Zumft W G.1997.Cell biology and molecular basis of denitrification[J].Microbiology and Molecular Biology Reviews,61(4):533-616
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