水鬼蕉生物碱对人肝癌HepG-2细胞凋亡的影响

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英文篇名：Effects of Alkaloids of Hymenocallis littoralis on Apoptosis of Human Hepatocellular Carcinoma HepG-2 Cells 作者：陈宁 ; 赵丹 ; 郑威 ; 李均 ; 杜娟 ; 凌娜 ; 宋冬雪 ; 徐昶儒 英文作者：CHEN Ning;ZHAO Dan;ZHENG Wei;LI Jun;DU Juan;LING Na;SONG Dong-xue;XU Chang-ru;Life Sciences and Environmental Sciences Research Center; 关键词：水鬼蕉生物碱 ; FITC-Annexin ; V/PI双染色法 ; Ca2+浓度 ; 活性氧 ; pH 英文关键词：alkaloids of Hymenocallis littoralis;;FITC-Annexin V/PI double staining method;;Ca2+concentration;;reactive oxygen species;;pH 中文刊名：黑龙江农业科学 英文刊名：Heilongjiang Agricultural Sciences 机构：哈尔滨商业大学生命科学与环境科学研究中心; 出版日期：2019-06-10 出版单位：黑龙江农业科学 年：2019 期：06 基金：黑龙江省博士后基金(LBH-Z16095);; 黑龙江省自然基金(H2017 001);; 哈尔滨市应用技术研究与开发项目(2016RQQXJ127) 语种：中文; 页：134-138 页数：5 CN：23-1204/S ISSN：1002-2767 分类号：R285

摘要

为揭示水鬼蕉生物碱(AHL)对HepG-2细胞作用,采用FITC-Annexin V/PI双染色法检测AHL对HepG-2细胞凋亡的影响,并检测了AHL对HepG-2细胞内Ca~(2+)浓度、活性氧(ROS)、pH的影响。结果表明:AHL可以通过细胞内ROS、Ca~(2+)、pH三者间相互作用而诱导细胞凋亡。
        In order to reveal the effect of alkaloids of Amaryllidaceae plant Hymenocallis littoralis(AHL)on HepG-2 cells,FITC-Annexin V/PI double staining was used to detect the effect of AHL on apoptosis of HepG-2 cells and on Ca~(2+) concentration,reactive oxygen species(ROS)and pH in HepG-2 cells.The results show that:AHL could induce apoptosis through the interaction of ROS,Ca~(2+),and pH in cells.

引文

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