马铃薯疮痂病拮抗菌Bacillus amyloliquefaciens12-82发酵条件优化
|
摘要
马铃薯疮痂病(Potato scab)是由链霉菌(Steptomyces spp.)引起的重要病害。前期工作中筛选到一株对马铃薯疮痂病菌具有较强拮抗活性的菌株Bacillus amyloliquefaciens 12-82。本研究通过响应面优化方法对该拮抗菌产芽孢条件进行优化,以满足生产的需要。结果表明,当培养基组成为2%玉米粉、2%蛋白胨、0.5%CaCl_2;培养条件为发酵时间48 h,装瓶量50 mL/150 mL,接种量10%,初始pH值7.3,培养温度33℃,芽孢数量可达到3.1×10~8 CFU/mL,实验结果对该菌株的开发应用具有一定指导意义。
Potato scab is an economic disease caused by Steptomyces spp. In previous work, a strain named Bacillus amyloliquefaciens 12-82, which had a good antagonistic resistance to potato scab, was screened. In order to meet the needs of production, this study optimized the spore production conditions of the antagonistic bacteria using response surface optimization. Results showed that the medium consisted of 2% cornmeal, 2% peptone and 0.5% CaCl_2, culture conditions were fermentation time 48 h, bottle filling amount to 50 mL/150 mL, inoculation quantity 10%, initial pH 7.3, culture temperature 33℃, the spore number was 3.1×10~8 CFU/mL, the experimental results will provide certain guiding significance for the development and application of the strain.
Potato scab is an economic disease caused by Steptomyces spp. In previous work, a strain named Bacillus amyloliquefaciens 12-82, which had a good antagonistic resistance to potato scab, was screened. In order to meet the needs of production, this study optimized the spore production conditions of the antagonistic bacteria using response surface optimization. Results showed that the medium consisted of 2% cornmeal, 2% peptone and 0.5% CaCl_2, culture conditions were fermentation time 48 h, bottle filling amount to 50 mL/150 mL, inoculation quantity 10%, initial pH 7.3, culture temperature 33℃, the spore number was 3.1×10~8 CFU/mL, the experimental results will provide certain guiding significance for the development and application of the strain.
引文
[1] 陈书珍,陈泰祥,季绪霞,等.甘肃省岷县马铃薯窖藏干腐病鉴定及药剂筛选[J],草业科学,2017
[2] 杜魏甫.云南省马铃薯疮痂病菌鉴定及品种资源抗性评价[J],云南农业大学,2016
[3] 刘方春,邢尚军,丁延芹,等.枯草芽孢杆菌DZ1的培养基以及发酵条件优化研究[J],生物灾害研究,2013.36(4):389-393.
[4] 李栓曹,李存玲.马铃薯疮痂病的发生与防治[J],陕西农业科学,2015(1):76-77.
[5] 刘大群.拮抗链霉菌防治马铃薯疮痂病的大田试验研究(英文)[J],植物病理学报,2000,30(3):237-244
[6] King R R,Lawrence C H,Clark M C.Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers [J].American Journal of Potato Research,1991,68:675-680.
[7] Hiltunen L,Laakso I,Chobot V,et al.The influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures [J].Journal of Agricultural and Food Chemistry,2006,54(9):3372-3379.
[8] 王针针,沈艳芬,陈家吉,等.中国马铃薯疮痂病研究进展[J],马铃薯产业与精准扶贫,2017
[9] Hiltunen L,Weckman A,Ylhainen A,et al.Responses of potato cultivars to the common scab pathogens,Streptomyces scabies and S.turgidiscabies [J].Annals of Applied Biology,2005,146:395-403.
[10] 张铭铄.一株马铃薯疮痂病病原菌的系统鉴定及生物防治的研究[D],山东农业大学,2016
[11] 王晨波.芽孢杆菌生长特性的研究[A],雀巢研发中心上海有限公司,2012
[12] Yuki O.Kobayashi,Akira Kobayashi,Masayuki Maeda,Shigehito Takenaka.Isolation of antagonistic Streptomyces sp.against a potato scab pathogen from a field cultivated with wild oat.J Gen Plant Pathol (2012) 78:62-72
[13] 惠明,窦丽娜,田青,等.枯草芽孢杆菌的应用研究进展[J],安徽农业科学,2008,36(27):11623-11624,11627
[14] Han JS,Cheng JH,Yoon TM,Song J,Rajkarnikar A,Kim WG,Yoo ID,Yang YY,Suh JW.Biological control agent of common scab disease by antagonistic strain Bacillus sp.sunhua.J Appl Microbiol 99(1):213-221
[15] 高同国,姜军坡,郭晓军,等.马铃薯疮痂病高效拮抗菌的筛选及鉴定[J],江苏农业科学,2016,44(12):157-159
[2] 杜魏甫.云南省马铃薯疮痂病菌鉴定及品种资源抗性评价[J],云南农业大学,2016
[3] 刘方春,邢尚军,丁延芹,等.枯草芽孢杆菌DZ1的培养基以及发酵条件优化研究[J],生物灾害研究,2013.36(4):389-393.
[4] 李栓曹,李存玲.马铃薯疮痂病的发生与防治[J],陕西农业科学,2015(1):76-77.
[5] 刘大群.拮抗链霉菌防治马铃薯疮痂病的大田试验研究(英文)[J],植物病理学报,2000,30(3):237-244
[6] King R R,Lawrence C H,Clark M C.Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers [J].American Journal of Potato Research,1991,68:675-680.
[7] Hiltunen L,Laakso I,Chobot V,et al.The influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures [J].Journal of Agricultural and Food Chemistry,2006,54(9):3372-3379.
[8] 王针针,沈艳芬,陈家吉,等.中国马铃薯疮痂病研究进展[J],马铃薯产业与精准扶贫,2017
[9] Hiltunen L,Weckman A,Ylhainen A,et al.Responses of potato cultivars to the common scab pathogens,Streptomyces scabies and S.turgidiscabies [J].Annals of Applied Biology,2005,146:395-403.
[10] 张铭铄.一株马铃薯疮痂病病原菌的系统鉴定及生物防治的研究[D],山东农业大学,2016
[11] 王晨波.芽孢杆菌生长特性的研究[A],雀巢研发中心上海有限公司,2012
[12] Yuki O.Kobayashi,Akira Kobayashi,Masayuki Maeda,Shigehito Takenaka.Isolation of antagonistic Streptomyces sp.against a potato scab pathogen from a field cultivated with wild oat.J Gen Plant Pathol (2012) 78:62-72
[13] 惠明,窦丽娜,田青,等.枯草芽孢杆菌的应用研究进展[J],安徽农业科学,2008,36(27):11623-11624,11627
[14] Han JS,Cheng JH,Yoon TM,Song J,Rajkarnikar A,Kim WG,Yoo ID,Yang YY,Suh JW.Biological control agent of common scab disease by antagonistic strain Bacillus sp.sunhua.J Appl Microbiol 99(1):213-221
[15] 高同国,姜军坡,郭晓军,等.马铃薯疮痂病高效拮抗菌的筛选及鉴定[J],江苏农业科学,2016,44(12):157-159
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |