抗玉米蚜玉米内生菌的筛选
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摘要
为探索防治玉米蚜的新方法,从山东省玉米主产区玉米植株上分离获得61株内生菌菌株,室内测定了对玉米蚜的防治效果,筛选出高效菌株,并测定了菌株对玉米生长的影响及在玉米植株内的定殖率。结果显示,白僵菌属菌株YC1和链格孢属菌株GX5对玉米蚜的防治效果最好,均为74.80%,无虫株率分别为32.22%和55.64%;接入菌株YC1和GX5后玉米各组织的生物量均大于对照,菌株YC1处理组的根、茎、叶干重分别增长了9.64%、15.71%和20.51%,菌株GX5处理组的根、茎、叶干重分别增长了3.88%、8.16%和9.14%;菌株GX5在玉米根、茎、叶中的定殖率可以达到44.23%以上,菌株YC1在玉米茎中的定殖率可以达到63.44%,有较大的应用价值。
To explore new strategies for sustainable control of corn aphid Rhopalosiphum maidis(Fitch) population,61 endophyte strains were collected from the major corn producing areas in Shandong province, and their control efficacies against R. maidis were determined under laboratory conditions to screen the highly effective strains.Meanwhile, the effects and rates of colonization of the strains in different tissues of corn were detected. The results showed that strains YC1 and GX5 had the best control efficacy of 74.80%, with rates of no-damage plants of32.22% and 55.64%, respectively. The biomass of corn tissues inoculated with stains YC1 and GX5 were higher than the control, dry weight of the root, stem and leaf of the YC1 treatment group increased by 9.64%, 15.71% and20.51%, respectively, and that of the GX5 treatment group increased by 3.88%, 8.16% and 9.14%, respectively. The colonization rates of strain GX5 in roots, stems and leaves were more than 44.23%, and the colonization rate of YC1 in stems was more than 63.44%. The results show that the strains GX5 and YC1 have great application potential.
To explore new strategies for sustainable control of corn aphid Rhopalosiphum maidis(Fitch) population,61 endophyte strains were collected from the major corn producing areas in Shandong province, and their control efficacies against R. maidis were determined under laboratory conditions to screen the highly effective strains.Meanwhile, the effects and rates of colonization of the strains in different tissues of corn were detected. The results showed that strains YC1 and GX5 had the best control efficacy of 74.80%, with rates of no-damage plants of32.22% and 55.64%, respectively. The biomass of corn tissues inoculated with stains YC1 and GX5 were higher than the control, dry weight of the root, stem and leaf of the YC1 treatment group increased by 9.64%, 15.71% and20.51%, respectively, and that of the GX5 treatment group increased by 3.88%, 8.16% and 9.14%, respectively. The colonization rates of strain GX5 in roots, stems and leaves were more than 44.23%, and the colonization rate of YC1 in stems was more than 63.44%. The results show that the strains GX5 and YC1 have great application potential.
引文
[1]李丽莉,王振营,何康来,等.转Bt基因抗虫玉米对玉米蚜种群增长的影响[J].应用生态学报,2007,18(5):1077-1080.
[2]赵曼,郭线茹,李为争,等.不同玉米品种(系)对玉米蚜生长发育和种群增长的影响[J].生态学报,2013,33(15):4707-4714.
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[5]Fuchsberg J R,Yong T H,Losey J E,et al.Evaluation of corn leaf aphid(Rhopalosiphum maidis;Homoptera:Aphididae)honeydew as a food source for the egg parasitoid Trichogramma ostriniae(Hymenoptera:Trichogrammatidae)[J].Biological Control,2007,40(2):230-236.
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[8]Azevedo J L,Maccheroni Jr W,Pereira J O,et al.Endophytic microorganisms:a review on insect control and recent advances on tropical plants[J].Electronic Journal of Biotechnology,2000,3(1):40-65.
[9]Kanda K,Hirai Y,Koga H,et al.Endophyte-enhanced resistance in perennial ryegrass and tall fescue to bluegrass webworm[J].Japanese Society of Applied Entomology and Zoology,1994,38:141-145.
[10]Funk C R,White R H,Breen J P.Importance of Acremonium endophytes in turf-grass breeding and management[J].Agriculture,Ecosystems&Environment,1993,44:215-232.
[11]赵龙飞,徐亚军,常佳丽.轮纹病菌拮抗性大豆根瘤内生菌的筛选、抗性和促生作用[J].中国生物防治学报,2016,32(3):396-405.
[12]Gurulingappa P,Sword G A,Murdoch G,et al.Colonization of crop plants by fungal entomopathogens and their effects on two insect pests when in planta[J].Biological Control,2010,55(1):34-41.
[13]Pampapathy G,Peter A M,Gregory S.Endophytic Lecanicillium lecanii and Beauveria bassiana reduce the survival and fecundity of Aphis gossypii following contact with conidia and secondary metabolites[J].Crop Protection,2011,30:349-353.
[14]宋海燕,李丽莉,卢增斌,等.山东省不同地区棉花内生真菌的多样性和群落结构分析[J].棉花学报,2018,30(5):363-374.
[15]George C C,Fanny E C.Studies on the incidence of coniferous needle endophytes in the Pacific Northwest[J].Canadian Journal of Botany,1978,56:3034-3043.
[16]李应德,闫智臣,高萍,等.摩西球囊霉对紫花苜蓿烟色织孢霉根腐病的影响[J].中国生物防治学报,2018,34(4):598-605.
[17]孙艺昕,门兴元,李超,等.双委夜蛾高致病力球孢白僵菌的筛选及在玉米内定殖率的测定[J].中国生物防治学报,2019,35(1):70-74.
[18]文才艺,吴元华,田秀玲.植物内生菌研究进展及其存在的问题[J].生态学杂志,2004,23(2):86-91.
[19]Parsa S,Ortiz V,Vega F E.Establishing fungal entomopathogens as endophytes:towards endophytic biological control[J].Journal of Visualized Experiments,2013,74:e50360.
[20]李晓慧,汪洋洲,张军,等.不同交配型球孢白僵菌在玉米植株中的定殖及其交配型基因变异比较研究[J].中国生物防治学报,2014,30(6):743-749.
[2]赵曼,郭线茹,李为争,等.不同玉米品种(系)对玉米蚜生长发育和种群增长的影响[J].生态学报,2013,33(15):4707-4714.
[3]So Y S,Ji H C,Brewbaker J L.Resistance to corn leaf aphid(Rhopalosiphum maidis Fitch)in tropical corn(Zea mays L.)[J].Euphytica,2010,172(3):373-381.
[4]赵玖华,尚佑芬,路兴波,等.田间蚜虫消长与玉米矮花叶病流行的相关性研究[J].山东农业科学,2003(1):30-31.
[5]Fuchsberg J R,Yong T H,Losey J E,et al.Evaluation of corn leaf aphid(Rhopalosiphum maidis;Homoptera:Aphididae)honeydew as a food source for the egg parasitoid Trichogramma ostriniae(Hymenoptera:Trichogrammatidae)[J].Biological Control,2007,40(2):230-236.
[6]王永宏,苏丽,仵均祥.温度对玉米蚜种群增长的影响[J].昆虫知识,2002,39(4):277-280.
[7]Stone J K,Bacon C W,White J F J.An overview of endophytic microbes:edophytism defined[J].Microbial Endophytes,2000(3):29-33.
[8]Azevedo J L,Maccheroni Jr W,Pereira J O,et al.Endophytic microorganisms:a review on insect control and recent advances on tropical plants[J].Electronic Journal of Biotechnology,2000,3(1):40-65.
[9]Kanda K,Hirai Y,Koga H,et al.Endophyte-enhanced resistance in perennial ryegrass and tall fescue to bluegrass webworm[J].Japanese Society of Applied Entomology and Zoology,1994,38:141-145.
[10]Funk C R,White R H,Breen J P.Importance of Acremonium endophytes in turf-grass breeding and management[J].Agriculture,Ecosystems&Environment,1993,44:215-232.
[11]赵龙飞,徐亚军,常佳丽.轮纹病菌拮抗性大豆根瘤内生菌的筛选、抗性和促生作用[J].中国生物防治学报,2016,32(3):396-405.
[12]Gurulingappa P,Sword G A,Murdoch G,et al.Colonization of crop plants by fungal entomopathogens and their effects on two insect pests when in planta[J].Biological Control,2010,55(1):34-41.
[13]Pampapathy G,Peter A M,Gregory S.Endophytic Lecanicillium lecanii and Beauveria bassiana reduce the survival and fecundity of Aphis gossypii following contact with conidia and secondary metabolites[J].Crop Protection,2011,30:349-353.
[14]宋海燕,李丽莉,卢增斌,等.山东省不同地区棉花内生真菌的多样性和群落结构分析[J].棉花学报,2018,30(5):363-374.
[15]George C C,Fanny E C.Studies on the incidence of coniferous needle endophytes in the Pacific Northwest[J].Canadian Journal of Botany,1978,56:3034-3043.
[16]李应德,闫智臣,高萍,等.摩西球囊霉对紫花苜蓿烟色织孢霉根腐病的影响[J].中国生物防治学报,2018,34(4):598-605.
[17]孙艺昕,门兴元,李超,等.双委夜蛾高致病力球孢白僵菌的筛选及在玉米内定殖率的测定[J].中国生物防治学报,2019,35(1):70-74.
[18]文才艺,吴元华,田秀玲.植物内生菌研究进展及其存在的问题[J].生态学杂志,2004,23(2):86-91.
[19]Parsa S,Ortiz V,Vega F E.Establishing fungal entomopathogens as endophytes:towards endophytic biological control[J].Journal of Visualized Experiments,2013,74:e50360.
[20]李晓慧,汪洋洲,张军,等.不同交配型球孢白僵菌在玉米植株中的定殖及其交配型基因变异比较研究[J].中国生物防治学报,2014,30(6):743-749.
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