金花茶遗传多样性和遗传结构AFLP分析
|
摘要
利用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分子标记技术,对金花茶(Camellia nitidissima) 4个天然种群的遗传多样性和遗传结构进行研究,为金花茶资源的有效保护和高效利用提供科学依据。结果表明,8对引物共扩增出1 619个DNA位点,其中1 473个位点具多态性,多态性位点百分率为90. 98%。金花茶物种水平基因多样性指数和Shannon信息指数为0. 146和0. 246,种群水平基因多样性指数和Shannon信息指数为0. 131和0. 215; 4个金花茶种群的多态位点百分率、基因多样性指数和Shannon信息指数变化趋势一致,其中多态位点百分率介于56. 83%～72. 11%之间,基因多样性指数介于0. 122～0. 138之间,Shannon信息指数介于0. 197～0. 228之间。金花茶种群间的遗传分化系数介于0. 139～0. 289之间,遗传变异主要存在于种群内。分子变异分析(AMOVA)结果表明,金花茶种群内遗传变异为81. 52%,种群间遗传变异为18. 48%,遗传变异主要存在于种群内。非加权组平均法(UPGMA)聚类结果表明,种群间遗传变异与其地理距离无明显相关性。应加强现有金花茶种群的就地保护,促进种群自然更新,同时加强迁地保护。
To provide the scientific basis for effective protection and efficient utilization of Camellia nitidissima resource,amplified fragment length polymorphism( AFLP) technology were used to study the genetic diversity and genetic structure of the four natural populations of C. nitidissima. The result show that 1 619 DNA loci were obtained using eight primers,of which,1 473 loci were polymorphic,and the percentage of polymorphism was 90. 98%. The gene diversity and Shannon's information index were 0. 146 and 0. 246,respectively,at specific level,and that were 0. 131 and 0. 215 at population level. The change trends of percentage of polymorphic loci,gene diversity and Shannon's information index of four C. nitidissima populations were uniform. The percentage of polymorphic loci ranged from 56. 83% to 72. 11%. The gene diversity index ranged from 0. 122 to 0. 138,and the Shannon's information index ranged from 0. 197 to 0. 228. The genetic differentiation coefficient of C. nitidissima populations was between 0. 139 and 0. 289,that shows the genetic variation of C. nitidissima was mainly within the populations. The analysis of molecular variance( AMOVA) showed 81. 52% of the genetic variation was within the populations,and 18. 48% of that was among the populations. The genetic variation of C. nitidissima was mainly within the populations. The unweighted pair group method with arithmetic mean( UPGMA) indicates that there was no significant correlation between the genetic variation of C. nitidissima populations and the geographic distance.Conservation strategies including in situ preservation for promoting natural populations regeneration and ex situ preservation of populations have been put forward.
To provide the scientific basis for effective protection and efficient utilization of Camellia nitidissima resource,amplified fragment length polymorphism( AFLP) technology were used to study the genetic diversity and genetic structure of the four natural populations of C. nitidissima. The result show that 1 619 DNA loci were obtained using eight primers,of which,1 473 loci were polymorphic,and the percentage of polymorphism was 90. 98%. The gene diversity and Shannon's information index were 0. 146 and 0. 246,respectively,at specific level,and that were 0. 131 and 0. 215 at population level. The change trends of percentage of polymorphic loci,gene diversity and Shannon's information index of four C. nitidissima populations were uniform. The percentage of polymorphic loci ranged from 56. 83% to 72. 11%. The gene diversity index ranged from 0. 122 to 0. 138,and the Shannon's information index ranged from 0. 197 to 0. 228. The genetic differentiation coefficient of C. nitidissima populations was between 0. 139 and 0. 289,that shows the genetic variation of C. nitidissima was mainly within the populations. The analysis of molecular variance( AMOVA) showed 81. 52% of the genetic variation was within the populations,and 18. 48% of that was among the populations. The genetic variation of C. nitidissima was mainly within the populations. The unweighted pair group method with arithmetic mean( UPGMA) indicates that there was no significant correlation between the genetic variation of C. nitidissima populations and the geographic distance.Conservation strategies including in situ preservation for promoting natural populations regeneration and ex situ preservation of populations have been put forward.
引文
[1]傅立国,金鉴明.中国植物红皮书:稀有濒危植物(第一册)[M].北京:科学出版社,1992:648-649.
[2]梁盛业.金花茶[M].北京:中国林业出版社,1993:1-21.
[3]HE D Y,LI X Y,XUAN S,et al.Camellia nitidissima,C.W.Chi:A Review of Botany,Chemistry and Pharmacology[J].Phytochemistry Reviews,2018,17(2):327-349.
[4]STINCHCOMBE J R,HOEKSTRA H E.Combining Population Genomics and Quantitative Genetics:Finding the Genes Underlying Ecologically Important Traits[J].Heredity,2008,100(2):158-170.
[5]QIU Y X,HONG D Y,FU C X,et al.Genetic Variation in the Endangered and Endemic Species Changium smyrnioides(Apiaceae)[J].Biochemical Systematics and Ecology,2004,32(6):583-596.
[6]宾晓芸,唐绍清,周俊亚,等.金花茶遗传多样性的ISSR分析[J].武汉植物学研究,2005,23(1):20-26.[BIN Xiao-yun,TANG Shao-qing,ZHOU Jun-ya,et al.ISSR Analysis on Genetic Diversity of Camellia nitidissima Chi(Theaceae)in China[J].Journal of Wuhan Botanical Research,2005,23(1):20-26.]
[7]TANG S Q,BIN X Y,WANG L,et al.Genetic Diversity and Population Structure of Yellow Camellia(Camellia nitidissima)in China as Revealed by RAPD and AFLP Markers[J].Biochemical Genetics,2006,44(9/10):449-461.
[8]WEI X,CAO H L,JIANG Y S,et al.Population Genetic Structure of Camellia nitidissima(Theaceae)and Conservation Implications[J].Botanical Studies,2008,49(2):147-153.
[9]韦霄,韦记青,蒋水元,等.迁地保护的金花茶遗传多样性评价[J].广西植物,2005,25(3):215-218.[WEI Xiao,WEI Ji-qing,JIANG Shui-yuan,et al.Genetic Diversity Evaluation of Ex-Situ Populations of Camellia nitidssima,Detected by ISSR Markers[J].Guihaia,2005,25(3):215-218.]
[10]ROSENDAHL S,TAYLOR J W.Development of Multiple Genetic Markers for Studies of Genetic Variation in Arbuscular Mycorrhizal Fungi Using AFLPTM[J].Molecular Ecology,1997,6(9):821-829.
[11]CHENG L,LIU L,YU X,et al.A Linkage Map of Common Carp(Cyprinus carpio)Based on AFLP and Microsatellite Markers[J].Animal Genetics,2009,41(2):191-198.
[12]ESCARAVAGE N,QUESTIAU S,PORNON A,et al.Clonal Diversity in a Rhododendron ferrugineum L.(Ericaceae)Population Inferred From AFLP Markers[J].Molecular Ecology,1998,7(8):975-982.
[13]JOSHI S P,GUPTA V S,AGGARWAL P K,et al.Genetic Diversity and Phylogenetic Relationship as Revealed by Inter Simple Sequence Repeat(ISSR)Polymorphism in the Genus Oryza[J].Theoretical and Applied Genetics,2000,100(8):1311-1320.
[14]邱芳,伏健民,金德敏,等.遗传多样性的分子检测[J].生物多样性,1998,6(2):143-150.[QIU Fang,FU Jian-min,JIN Demin,et al.The Molecular Detection of Genetic Diversity[J].Chinese Biodiversity,1998,6(2):143-150.]
[15]王建波.ISSR分子标记及其在植物遗传学研究中的应用[J].遗传,2002,24(5):613-616.[WANG Jian-bo.ISSR Markers and Their Application in Plant Genetics[J].Hereditas,2002,24(5):613-616.]
[16]NYBOM H.Comparison of Different Nuclear DNA Markers for Estimating Intraspecific Genetic Diversity in Plants[J].Molecular E-cology,2004,13(5):1143-1155.
[17]LIN L,HU Z Y,NI S,et al.Genetic Diversity of Camellia japonica(Theaceae),a Species Endangered to East Asia,Detected by Inter-Simple Sequence Repeat(ISSR)[J].Biochemical Systematics and Ecology,2013,50:199-206.
[18]柴胜丰,庄雪影,邹蓉,等.濒危植物毛瓣金花茶遗传多样性的ISSR分析[J].西北植物学报,2014,34(1):93-98.[CHAISheng-feng,ZHUANG Xue-ying,ZOU Rong,et al.Genetic Diversity Analysis of Endangered Plant Camellia pubipetala Detected by ISSR[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(1):93-98.]
[19]李辛雷,孙振元,李纪元,等.广东鹅凰嶂自然保护区杜鹃红山茶种群结构与空间分布格局[J].应用生态学报,2013,24(8):2115-2121.[LI Xin-lei,SUN Zhen-yuan,LI Ji-yuan,et al.Population Structure and Spatial Distribution Pattern of Camellia azalea in E'huangzhang Nature Reserve of Guangdong,China[J].Chinese Journal of Applied Ecology,2013,24(8):2115-2121.]
[20]罗晓莹,唐光大,许涵,等.山茶科3种中国特有濒危植物的遗传多样性研究[J].生物多样性,2005,13(2):112-121.[LUOXiao-ying,TANG Guang-da,XU Han,et al.Genetic Diversity of Three Endemic and Endangered Species of the Family Theaceae in Guangdong,China[J].Biodiversity Science,2005,13(2):112-121.]
[21]罗晓莹,庄雪影,杨跃生.杜鹃红山茶遗传多样性的ISSR分析[J].热带亚热带植物学报,2007,15(2):93-100.[LUO Xiaoying,ZHUANG Xue-ying,YANG Yue-sheng.Genetic Diversity of Camellia changii Ye(Theaceae)Using ISSR Markers[J].Journal of Tropical and Subtropical Botany,2007,15(2):93-100.]
[2]梁盛业.金花茶[M].北京:中国林业出版社,1993:1-21.
[3]HE D Y,LI X Y,XUAN S,et al.Camellia nitidissima,C.W.Chi:A Review of Botany,Chemistry and Pharmacology[J].Phytochemistry Reviews,2018,17(2):327-349.
[4]STINCHCOMBE J R,HOEKSTRA H E.Combining Population Genomics and Quantitative Genetics:Finding the Genes Underlying Ecologically Important Traits[J].Heredity,2008,100(2):158-170.
[5]QIU Y X,HONG D Y,FU C X,et al.Genetic Variation in the Endangered and Endemic Species Changium smyrnioides(Apiaceae)[J].Biochemical Systematics and Ecology,2004,32(6):583-596.
[6]宾晓芸,唐绍清,周俊亚,等.金花茶遗传多样性的ISSR分析[J].武汉植物学研究,2005,23(1):20-26.[BIN Xiao-yun,TANG Shao-qing,ZHOU Jun-ya,et al.ISSR Analysis on Genetic Diversity of Camellia nitidissima Chi(Theaceae)in China[J].Journal of Wuhan Botanical Research,2005,23(1):20-26.]
[7]TANG S Q,BIN X Y,WANG L,et al.Genetic Diversity and Population Structure of Yellow Camellia(Camellia nitidissima)in China as Revealed by RAPD and AFLP Markers[J].Biochemical Genetics,2006,44(9/10):449-461.
[8]WEI X,CAO H L,JIANG Y S,et al.Population Genetic Structure of Camellia nitidissima(Theaceae)and Conservation Implications[J].Botanical Studies,2008,49(2):147-153.
[9]韦霄,韦记青,蒋水元,等.迁地保护的金花茶遗传多样性评价[J].广西植物,2005,25(3):215-218.[WEI Xiao,WEI Ji-qing,JIANG Shui-yuan,et al.Genetic Diversity Evaluation of Ex-Situ Populations of Camellia nitidssima,Detected by ISSR Markers[J].Guihaia,2005,25(3):215-218.]
[10]ROSENDAHL S,TAYLOR J W.Development of Multiple Genetic Markers for Studies of Genetic Variation in Arbuscular Mycorrhizal Fungi Using AFLPTM[J].Molecular Ecology,1997,6(9):821-829.
[11]CHENG L,LIU L,YU X,et al.A Linkage Map of Common Carp(Cyprinus carpio)Based on AFLP and Microsatellite Markers[J].Animal Genetics,2009,41(2):191-198.
[12]ESCARAVAGE N,QUESTIAU S,PORNON A,et al.Clonal Diversity in a Rhododendron ferrugineum L.(Ericaceae)Population Inferred From AFLP Markers[J].Molecular Ecology,1998,7(8):975-982.
[13]JOSHI S P,GUPTA V S,AGGARWAL P K,et al.Genetic Diversity and Phylogenetic Relationship as Revealed by Inter Simple Sequence Repeat(ISSR)Polymorphism in the Genus Oryza[J].Theoretical and Applied Genetics,2000,100(8):1311-1320.
[14]邱芳,伏健民,金德敏,等.遗传多样性的分子检测[J].生物多样性,1998,6(2):143-150.[QIU Fang,FU Jian-min,JIN Demin,et al.The Molecular Detection of Genetic Diversity[J].Chinese Biodiversity,1998,6(2):143-150.]
[15]王建波.ISSR分子标记及其在植物遗传学研究中的应用[J].遗传,2002,24(5):613-616.[WANG Jian-bo.ISSR Markers and Their Application in Plant Genetics[J].Hereditas,2002,24(5):613-616.]
[16]NYBOM H.Comparison of Different Nuclear DNA Markers for Estimating Intraspecific Genetic Diversity in Plants[J].Molecular E-cology,2004,13(5):1143-1155.
[17]LIN L,HU Z Y,NI S,et al.Genetic Diversity of Camellia japonica(Theaceae),a Species Endangered to East Asia,Detected by Inter-Simple Sequence Repeat(ISSR)[J].Biochemical Systematics and Ecology,2013,50:199-206.
[18]柴胜丰,庄雪影,邹蓉,等.濒危植物毛瓣金花茶遗传多样性的ISSR分析[J].西北植物学报,2014,34(1):93-98.[CHAISheng-feng,ZHUANG Xue-ying,ZOU Rong,et al.Genetic Diversity Analysis of Endangered Plant Camellia pubipetala Detected by ISSR[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(1):93-98.]
[19]李辛雷,孙振元,李纪元,等.广东鹅凰嶂自然保护区杜鹃红山茶种群结构与空间分布格局[J].应用生态学报,2013,24(8):2115-2121.[LI Xin-lei,SUN Zhen-yuan,LI Ji-yuan,et al.Population Structure and Spatial Distribution Pattern of Camellia azalea in E'huangzhang Nature Reserve of Guangdong,China[J].Chinese Journal of Applied Ecology,2013,24(8):2115-2121.]
[20]罗晓莹,唐光大,许涵,等.山茶科3种中国特有濒危植物的遗传多样性研究[J].生物多样性,2005,13(2):112-121.[LUOXiao-ying,TANG Guang-da,XU Han,et al.Genetic Diversity of Three Endemic and Endangered Species of the Family Theaceae in Guangdong,China[J].Biodiversity Science,2005,13(2):112-121.]
[21]罗晓莹,庄雪影,杨跃生.杜鹃红山茶遗传多样性的ISSR分析[J].热带亚热带植物学报,2007,15(2):93-100.[LUO Xiaoying,ZHUANG Xue-ying,YANG Yue-sheng.Genetic Diversity of Camellia changii Ye(Theaceae)Using ISSR Markers[J].Journal of Tropical and Subtropical Botany,2007,15(2):93-100.]
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |