高通量测序技术分析光叶珙桐根际土壤微生物多样性
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摘要
【目的】通过了解不同地区光叶珙桐根际土壤微生物的种类和多样性差异,为光叶珙桐的引种繁育提供基础数据和理论依据。【方法】采用高通量测序技术对昭通3个地区光叶珙桐根际土壤微生物种类及多样性进行研究,分析了物种组成和丰度、Alpha多样性、Beta多样性和菌群结构。【结果】结果表明,细菌群落中α-变形菌纲Alphaproteobacteria最丰富(> 17.02%),真菌群落在不同地区存在明显差异,在永善三江口Pezizomycetes最丰富(17.01%),彝良小草坝Archaeorhizomycetes最丰富(29.79%),镇雄塘房Sordariomycetes最丰富(20.77%);3个地区中永善三江口、镇雄塘房的光叶珙桐根际土壤微生物种群结构相似性较高,其中永善三江口的光叶珙桐根际土壤细菌多样性最丰富,镇雄塘房的光叶珙桐根际土壤真菌多样性最丰富。【结论】3个地区的光叶珙桐根际土壤微生物菌群都具有较高的生物多样性,但物种组成分布上存在差异。
【Objective】The rhizosphere soil of Davidia involucrate in different regions was tested in order to understand the species and diversity of the rhizosphere microorganisms, and provide basic data and theoretical basis for the introduction and breeding of D. involucrate. 【Method】High-throughput sequencing was applied to study the microbial species and diversity of rhizosphere soil of D. involucrate in three regions of Zhaotong, and the species composition and abundance, Alpha diversity, Beta diversity and microbial community structure were analyzed. 【Result】The results showed that Alphaproteobacteria was the most abundant in the bacterial community(>17.02%), Fungal community was obviously different in three regions. Pezizomycetes was the most abundant fungi class(17.01%) in Yongshansanjiangkou,Archaeorhizomycetes was the most abundant fungi class(29.79%) in Yiliangxiaocaoba, Sordariomycetes was the most abundant fungi class(20.77%) in Zhenxiongtangfang. Among the three regions, the microbial community structures in Sanjiangkou, Yongshan and Tangfang, Zhenxiong were highly similar. The rhizosphere soil in Sanjiangkou, Yongshan reflected the richest bacterial diversity, while that in Tangfang, Zhenxiong mirrored the richest fungal diversity. 【Conclusion】The microbial community in rhizosphere soil of D. involucrate in the three regions had high biodiversity, but there were differences in the distribution of species composition.
【Objective】The rhizosphere soil of Davidia involucrate in different regions was tested in order to understand the species and diversity of the rhizosphere microorganisms, and provide basic data and theoretical basis for the introduction and breeding of D. involucrate. 【Method】High-throughput sequencing was applied to study the microbial species and diversity of rhizosphere soil of D. involucrate in three regions of Zhaotong, and the species composition and abundance, Alpha diversity, Beta diversity and microbial community structure were analyzed. 【Result】The results showed that Alphaproteobacteria was the most abundant in the bacterial community(>17.02%), Fungal community was obviously different in three regions. Pezizomycetes was the most abundant fungi class(17.01%) in Yongshansanjiangkou,Archaeorhizomycetes was the most abundant fungi class(29.79%) in Yiliangxiaocaoba, Sordariomycetes was the most abundant fungi class(20.77%) in Zhenxiongtangfang. Among the three regions, the microbial community structures in Sanjiangkou, Yongshan and Tangfang, Zhenxiong were highly similar. The rhizosphere soil in Sanjiangkou, Yongshan reflected the richest bacterial diversity, while that in Tangfang, Zhenxiong mirrored the richest fungal diversity. 【Conclusion】The microbial community in rhizosphere soil of D. involucrate in the three regions had high biodiversity, but there were differences in the distribution of species composition.
引文
[1]钱崇澍,陈焕镛.中国植物志[M].北京:科学出版社,1983.QIAN C S,CHEN H Y.Flora of China[M].Beijing:Science Press,1983.
[2]胡进耀,苏智先,黎云祥.珙桐生物学研究进展[J].中国野生植物资源,2003,22(4):15-19.doi:10.3969/j.issn.1006-9690.2003.04.005.HU J Y,SU Z X,LI Y X.Research advance on dovetree biology[J].Chinese Wild Plant Resources,2003,22(4):15-19.doi:10.3969/j.issn.1006-9690.2003.04.005.
[3]张家勋,李俊清,周宝顺,廉秀荣.珙桐繁殖和栽培技术研究[J].北京林业大学学报,1995,17(3):24-29.doi:10.13332/j.1000-1522.1995.03.004.ZHANG J X,LI J Q,ZHOU B S,LIAN X R.Studies on the Chinese dovetree propagation and cultivation techniques[J].Journal of Beijing Forestry University,1995,17(3):24-29.doi:10.13332/j.1000-1522.1995.03.004.
[4]金晓玲,吴安湘,沈守云,章怀云.珍稀濒危植物珙桐离体快繁技术初步研究[J].园艺学报,2007,34(5):1327-1328.doi:10.3321/j.issn:0513-353x.2007.05.046.JIN X L,WU A X,SHEN S Y A,ZHANG H Y.Preliminary study on the in Vitro culture of endangered plant Davidia involucrata Baill.[J].Acta Horticulturae Sinica,2007,34(5):1327-1328.doi:10.3321/j.issn:0513-353x.2007.05.046.
[5]陈蕤坤,吴鹏飞,何青霞,周海滨,黄治宇,徐莺.珙桐花药诱导愈伤组织的初步研究[J].四川大学学报(自然科学版),2012,49(5):1137-1142.doi:10.3969/j.issn.0490-6756.2012.05.033.CHEN R K,WU P F,HE Q X,ZHOU H B,HUANG Z Y,XU Y.Preliminary study on the Davidia involucrata anther callus induction[J].Journal of Sichuan University(Natural Science Edition),2012,49(5):1137-1142.doi:10.3969/j.issn.0490-6756.2012.05.033.
[6]王宁宁,胡增辉,沈应柏.珙桐苗木叶片光合特性对土壤干旱胁迫的响应[J].西北植物学报,2011,31(1):101-108.WANG N N,HU Z H,SHEN Y B.Photosynthetic characteristics of Davidia involucrata Baill.seedlings under soil drought stress[J].Acta Botanica Boreali-occidentalia Sinica,2011,31(1):101-108.
[7]姜瑞芳,刘艳红.土壤基质和水分对珙桐幼苗生长的影响[J].西北林学院学报,2016,31(4):134-139.doi:10.3969/j.issn.1001-7461.2016.04.23.JIANG R F,LIU Y H.Effects of soil matrix and moisture on the growth of Davidia involucrata seedlings[J].Journal of Northwest Forestry University,2016,31(4):134-139.doi:10.3969/j.issn.1001-7461.2016.04.23.
[8]戴雅婷,闫志坚,王慧,吴洪新.油蒿根际土壤微生物数量及其与土壤养分的关系[J].中国草地学报,2012,34(2):71-75.doi:10.3969/j.issn.1673-5021.2012.02.012.DAI Y T,YAN Z J,WANG H,WU H X.The relationships between the number of microorganisms in rhizospheric soil of Artemisia ordosica and soil nutrients[J].Chinese Journal of Grassland,2012,34(2):71-75.doi:10.3969/j.issn.1673-5021.2012.02.012.
[9]张燕燕,曲来叶,陈利顶,卫伟.黄土丘陵沟壑区不同植被类型土壤微生物特性[J].应用生态学报,2010,21(1):165-173.doi:10.13287/j.1001-9332.2010.0047.ZHANG Y Y,QU L Y,CHEN L D,WEI W.Soil microbial properties under different vegetation types in Loess hilly region[J].Chinese Journal of Applied Ecology,2010,21(1):165-173.doi:10.13287/j.1001-9332.2010.0047.
[10]康林玉,刘周斌,欧立军,童辉,彭莹,袁祖华.辣椒种植对根际土壤微生物多样性的影响[J].湖南农业大学学报(自然科学版),2018,44(2):151-156.doi:10.13331/j.cnki.jhau.2018.02.007.KANG L Y,LIU Z B,OU L J,TONG H,PENG Y,YUAN Z H.Effects of pepper cultivation on the microbial diversity of rhizosphere soil[J].Journal of Hunan Agricultural University(Natural Sciences),2018,44(2):151-156.doi:10.13331/j.cnki.jhau.2018.02.007.
[11]Daffonchio D,Hirt H,Berg G.Plant-microbe interactions and water management in Arid and Saline Soils[M]//Principles of Plant-Microbe Interactions.Springer International Publishing,2015:265-276.
[12]李越鲲,孙燕飞,雷勇辉,周旋,尹跃,秦垦.枸杞根际土壤真菌群落多样性的高通量测序[J].微生物学报,2017,57(7):1049-1059.doi:10.13343/j.cnki.wsxb.20160410.LI Y K,SUN Y F,LEI Y H,ZHOU X,YIN Y,QIN K.Fungal community diversity in rhizosphere soil of Lycium barbarum L.based on high-throughput sequencing[J].Acta Microbiologica Sinica,2017,57(7):1049-1059.doi:10.13343/j.cnki.wsxb.20160410.
[13]王珊珊.胡杨林地土壤中促进植物耐旱生长微生物的筛选及其机理[D].上海:华东理工大学,2014.WANG S S.Screening and mechanism of microorganisms promoting drought tolerance of plants in Populus euphratica forest soil[D].Shanghai:East China University of Science and Technology,2014.
[14]李朕.丛枝菌根真菌(AMF)提高青杨雌雄株抗旱性的研究[D].杨凌:西北农林科技大学,2017.LI Z.Influence of Arbuscular Mucorrhizal Fungi(AMF)inoculation on drought tolerence of Populus cathayana Rehder males and females[D].Yangling:Northwest University of Agriculture and Forestry Science and Technology,2017.
[15]杨敬天,胡进耀,张涛,彭波,邓东周.珙桐土壤微生物数量及其与土壤因子的关系[J].江苏农业科学,2014,42(1):278-281.doi:10.3969/j.issn.1002-1302.2014.01.105.YANG J T,HU J Y,ZHANG T,PENG B,DENG D Z.Number of soil microorganisms in Davidia involucrata and its relationship with soil factors[J].Jiangsu Agricultural Sciences,2014,42(1):278-281.doi:10.3969/j.issn.1002-1302.2014.01.105.
[16]秦越,马琨,刘萍.马铃薯连作栽培对土壤微生物多样性的影响[J].中国生态农业学报,2015,23(2):225-232.doi:10.13930/j.cnki.cjea.140755.QIN Y,MA K,LIU P.Effect of potato continuous cropping on genetic diversity of soil microorganisms[J].Chinese Journal of EcoAgriculture,2015,23(2):225-232.doi:10.13930/j.cnki.cjea.140755.
[17]罗柏青,杜凡,王娟,石翠玉,赫尚丽.滇东北珙桐群落结构特征研究[J].林业调查规划,2009,34(1):15-20.doi:10.3969/j.issn.1671-3168.2009.01.005.LUO B Q,DU F,WANG J,SHI C Y,HE S L.Study on characteristics of community structure of Davidia involucrata in Northeastern Yunnan[J].Forest Inventory and Planning,2009,34(1):15-20.doi:10.3969/j.issn.1671-3168.2009.01.005.
[18]夏嘉禹,周庆宏,邓莉兰,赵亚玲,陈志华.镇雄县珙桐群落特征及种群更新研究[J].林业调查规划,2017,42(2):30-34.doi:10.3969/j.issn.1671-3168.2017.02.007.XIA J Y,ZHOU Q H,DENG L L,ZHAO Y L,CHEN Z H.Characteristics of community structure of Davidia involucrata in Zhenxiong County[J].Forest Inventory and Planning,2017,42(2):30-34.doi:10.3969/j.issn.1671-3168.2017.02.007.
[2]胡进耀,苏智先,黎云祥.珙桐生物学研究进展[J].中国野生植物资源,2003,22(4):15-19.doi:10.3969/j.issn.1006-9690.2003.04.005.HU J Y,SU Z X,LI Y X.Research advance on dovetree biology[J].Chinese Wild Plant Resources,2003,22(4):15-19.doi:10.3969/j.issn.1006-9690.2003.04.005.
[3]张家勋,李俊清,周宝顺,廉秀荣.珙桐繁殖和栽培技术研究[J].北京林业大学学报,1995,17(3):24-29.doi:10.13332/j.1000-1522.1995.03.004.ZHANG J X,LI J Q,ZHOU B S,LIAN X R.Studies on the Chinese dovetree propagation and cultivation techniques[J].Journal of Beijing Forestry University,1995,17(3):24-29.doi:10.13332/j.1000-1522.1995.03.004.
[4]金晓玲,吴安湘,沈守云,章怀云.珍稀濒危植物珙桐离体快繁技术初步研究[J].园艺学报,2007,34(5):1327-1328.doi:10.3321/j.issn:0513-353x.2007.05.046.JIN X L,WU A X,SHEN S Y A,ZHANG H Y.Preliminary study on the in Vitro culture of endangered plant Davidia involucrata Baill.[J].Acta Horticulturae Sinica,2007,34(5):1327-1328.doi:10.3321/j.issn:0513-353x.2007.05.046.
[5]陈蕤坤,吴鹏飞,何青霞,周海滨,黄治宇,徐莺.珙桐花药诱导愈伤组织的初步研究[J].四川大学学报(自然科学版),2012,49(5):1137-1142.doi:10.3969/j.issn.0490-6756.2012.05.033.CHEN R K,WU P F,HE Q X,ZHOU H B,HUANG Z Y,XU Y.Preliminary study on the Davidia involucrata anther callus induction[J].Journal of Sichuan University(Natural Science Edition),2012,49(5):1137-1142.doi:10.3969/j.issn.0490-6756.2012.05.033.
[6]王宁宁,胡增辉,沈应柏.珙桐苗木叶片光合特性对土壤干旱胁迫的响应[J].西北植物学报,2011,31(1):101-108.WANG N N,HU Z H,SHEN Y B.Photosynthetic characteristics of Davidia involucrata Baill.seedlings under soil drought stress[J].Acta Botanica Boreali-occidentalia Sinica,2011,31(1):101-108.
[7]姜瑞芳,刘艳红.土壤基质和水分对珙桐幼苗生长的影响[J].西北林学院学报,2016,31(4):134-139.doi:10.3969/j.issn.1001-7461.2016.04.23.JIANG R F,LIU Y H.Effects of soil matrix and moisture on the growth of Davidia involucrata seedlings[J].Journal of Northwest Forestry University,2016,31(4):134-139.doi:10.3969/j.issn.1001-7461.2016.04.23.
[8]戴雅婷,闫志坚,王慧,吴洪新.油蒿根际土壤微生物数量及其与土壤养分的关系[J].中国草地学报,2012,34(2):71-75.doi:10.3969/j.issn.1673-5021.2012.02.012.DAI Y T,YAN Z J,WANG H,WU H X.The relationships between the number of microorganisms in rhizospheric soil of Artemisia ordosica and soil nutrients[J].Chinese Journal of Grassland,2012,34(2):71-75.doi:10.3969/j.issn.1673-5021.2012.02.012.
[9]张燕燕,曲来叶,陈利顶,卫伟.黄土丘陵沟壑区不同植被类型土壤微生物特性[J].应用生态学报,2010,21(1):165-173.doi:10.13287/j.1001-9332.2010.0047.ZHANG Y Y,QU L Y,CHEN L D,WEI W.Soil microbial properties under different vegetation types in Loess hilly region[J].Chinese Journal of Applied Ecology,2010,21(1):165-173.doi:10.13287/j.1001-9332.2010.0047.
[10]康林玉,刘周斌,欧立军,童辉,彭莹,袁祖华.辣椒种植对根际土壤微生物多样性的影响[J].湖南农业大学学报(自然科学版),2018,44(2):151-156.doi:10.13331/j.cnki.jhau.2018.02.007.KANG L Y,LIU Z B,OU L J,TONG H,PENG Y,YUAN Z H.Effects of pepper cultivation on the microbial diversity of rhizosphere soil[J].Journal of Hunan Agricultural University(Natural Sciences),2018,44(2):151-156.doi:10.13331/j.cnki.jhau.2018.02.007.
[11]Daffonchio D,Hirt H,Berg G.Plant-microbe interactions and water management in Arid and Saline Soils[M]//Principles of Plant-Microbe Interactions.Springer International Publishing,2015:265-276.
[12]李越鲲,孙燕飞,雷勇辉,周旋,尹跃,秦垦.枸杞根际土壤真菌群落多样性的高通量测序[J].微生物学报,2017,57(7):1049-1059.doi:10.13343/j.cnki.wsxb.20160410.LI Y K,SUN Y F,LEI Y H,ZHOU X,YIN Y,QIN K.Fungal community diversity in rhizosphere soil of Lycium barbarum L.based on high-throughput sequencing[J].Acta Microbiologica Sinica,2017,57(7):1049-1059.doi:10.13343/j.cnki.wsxb.20160410.
[13]王珊珊.胡杨林地土壤中促进植物耐旱生长微生物的筛选及其机理[D].上海:华东理工大学,2014.WANG S S.Screening and mechanism of microorganisms promoting drought tolerance of plants in Populus euphratica forest soil[D].Shanghai:East China University of Science and Technology,2014.
[14]李朕.丛枝菌根真菌(AMF)提高青杨雌雄株抗旱性的研究[D].杨凌:西北农林科技大学,2017.LI Z.Influence of Arbuscular Mucorrhizal Fungi(AMF)inoculation on drought tolerence of Populus cathayana Rehder males and females[D].Yangling:Northwest University of Agriculture and Forestry Science and Technology,2017.
[15]杨敬天,胡进耀,张涛,彭波,邓东周.珙桐土壤微生物数量及其与土壤因子的关系[J].江苏农业科学,2014,42(1):278-281.doi:10.3969/j.issn.1002-1302.2014.01.105.YANG J T,HU J Y,ZHANG T,PENG B,DENG D Z.Number of soil microorganisms in Davidia involucrata and its relationship with soil factors[J].Jiangsu Agricultural Sciences,2014,42(1):278-281.doi:10.3969/j.issn.1002-1302.2014.01.105.
[16]秦越,马琨,刘萍.马铃薯连作栽培对土壤微生物多样性的影响[J].中国生态农业学报,2015,23(2):225-232.doi:10.13930/j.cnki.cjea.140755.QIN Y,MA K,LIU P.Effect of potato continuous cropping on genetic diversity of soil microorganisms[J].Chinese Journal of EcoAgriculture,2015,23(2):225-232.doi:10.13930/j.cnki.cjea.140755.
[17]罗柏青,杜凡,王娟,石翠玉,赫尚丽.滇东北珙桐群落结构特征研究[J].林业调查规划,2009,34(1):15-20.doi:10.3969/j.issn.1671-3168.2009.01.005.LUO B Q,DU F,WANG J,SHI C Y,HE S L.Study on characteristics of community structure of Davidia involucrata in Northeastern Yunnan[J].Forest Inventory and Planning,2009,34(1):15-20.doi:10.3969/j.issn.1671-3168.2009.01.005.
[18]夏嘉禹,周庆宏,邓莉兰,赵亚玲,陈志华.镇雄县珙桐群落特征及种群更新研究[J].林业调查规划,2017,42(2):30-34.doi:10.3969/j.issn.1671-3168.2017.02.007.XIA J Y,ZHOU Q H,DENG L L,ZHAO Y L,CHEN Z H.Characteristics of community structure of Davidia involucrata in Zhenxiong County[J].Forest Inventory and Planning,2017,42(2):30-34.doi:10.3969/j.issn.1671-3168.2017.02.007.
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