浮游植物叶绿素a测定方法研究进展
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摘要
叶绿素a作为评价水体富营养化水平及水生态健康的重要指标,其监测方式目前越来越受到环境监管部门及相关科研机构的重视。综述了目前常用的几种监测方法,并对每种方法的优劣进行了系统分析,发现每种监测方法都存在一定的限制条件。因此,环保部门进行例行监测或者科研机构进行长期跟踪监测时要根据监测对象的不同选择合适的方法,甚至选择几种监测方法结合的方式来进行叶绿素a的测定,以为水环境管理和科研工作提供可靠的数据支撑。
As an important indicator of water eutrophication and water ecological health, the monitoring methods of Chlorophyll-a are being increasingly valued by environmental supervision departments and relevant scientific research institutions. This paper reviews several commonly used monitoring methods, and systematically analyzes the advantages and disadvantages of each method. It is found that each monitoring method has certain restrictions. Therefore, when the environmental protection department conducts routine monitoring or scientific research institutions conduct long-term tracking monitoring, they should choose the appropriate method according to the different monitoring objects, or even combining different monitoring methods to determine chlorophyll-a, so as to provide reliable data support for water environmental management and scientific research work.
As an important indicator of water eutrophication and water ecological health, the monitoring methods of Chlorophyll-a are being increasingly valued by environmental supervision departments and relevant scientific research institutions. This paper reviews several commonly used monitoring methods, and systematically analyzes the advantages and disadvantages of each method. It is found that each monitoring method has certain restrictions. Therefore, when the environmental protection department conducts routine monitoring or scientific research institutions conduct long-term tracking monitoring, they should choose the appropriate method according to the different monitoring objects, or even combining different monitoring methods to determine chlorophyll-a, so as to provide reliable data support for water environmental management and scientific research work.
引文
[1] 金相灿, 屠清瑛. 湖泊富营养化调查规范[M]. 北京 : 中国环境科学出版社. 1990, 268-270.
[2] 万修志. 提取测定淡水藻中叶绿素a的方法研究[D].长春:山东建筑大学,2008.
[3] 刘建华. 荧光法测定水体中叶绿素含量影响因素的研究[D].石家庄:河北科技大学,2014.
[4] 国家环境保护总局. 水和废水监测分析方法(第4版)[M].北京: 中国环境科学出版社,2002: 670-672.
[5] 汪志国,王静,李国刚. 双波长分光广度法同时测定叶绿素[J]. 中国环境检测, 1999, 15(5):21-22.
[6] 韩桂春,谷丰,张忠臣. 淡水中叶绿素a测定方法的探讨[J].中国环境监测. 2005, 21(1): 55-57.
[7] 冯菁,李艳波,朱擎. 浮游植物叶绿素a测定方法比较[J]. 生态环境, 2008, 17(2): 524-527.
[8] 童桂凤,陈志芳,范莹.分光光度法测定叶绿素a的比较[J].环境监测管理与技术. 2012, 24(1): 53-55.
[9] 李会玲,赵锋,豆艳霞. 水体中叶绿素a测定方法的探究[J]. 分析测试,2017,48(3): 52-54.
[10] 王海黎,洪华生,徐立. 反相高效液相色谱法分离、测定海洋浮游植物的叶绿素和类胡萝卜素[J]. 海洋科学, 1999,(4): 6-9.
[11] 林少君,贺立静,黄沛生等. 浮游植物中叶绿素a提取方法的比较研究[J].生态科学, 2005, 24(1): 9-11.
[12] C. S.Yentsch, C. M. Yentsch. Fluorescence spectral Signatures: The Characterization of Phytoplankton Populations by Theuse of Excitation and Emission Spectra[J]. Mar. Res, 1979,37:471-483.
[13] Kaitala S, Babichenko S, Poryvkina L, Leebea A. Fluorescent analysis of pigment composition of natural phytoplankton[J]. Mar technol Soc J, 1994, 28: 50-58.
[14] Tsai-yun Lee, Mikio Tsuzuki. Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method[J]. Analytica Chimica Acta 1995,302: 81-87.
[15] D.Mckee,A. Cunningham, K. Jones. An integrated submersible Fluorometer/Nephelometer/Transmissometer:Design and Testing at Sea. Optics&Laser Technology, 1997,29(1):35-39.
[16] M. Beutler, K. H. Wiltshire, B. Meyer et al. A fluorometric Method for the Differentiation of Algal Populations in vivo and in situ. Photosynthesis Research, 2002,72(1):39-53.
[17] 金海龙. 基于荧光机理的海藻识别方法与实验研究[D].河北:燕山大学,2005.
[18] Bianchi,T.S. A comparion between fluorometric and high-performance liquid chromatography measurement[J]. Bulletin of marine science, 1995, 56(1):28-31.
[19] 王荣,鲁北纬,余家栋. 海水叶绿素a含量的连续走航测定[J]. 海洋与湖沼,1996,27(3):296-301.
[20] 郑龙江.光纤式海藻叶绿素a浓度的荧光测量理论与实验研究 [D].秦皇岛:燕山大学,2001.
[21] 唐尧基,游文玮,陈莹,等.同步荧光法测定海水中叶绿素 a 的含量[J].分析仪器,2004,(3):24-26.
[22] J.Gregor, B.Marsalek. Freshwater phytoplankton quantification by chlorophyll a: a comparative study of in vitro, in vivo and in situ methods[J]. Water Research. 2004,(38):517-522.
[23] 王刚,秦少平,晏光辉. LED 激发叶绿素荧光探测仪的设计研究[J].佳木斯大学学报(自然科学版),2010, 28(1): 39-41.
[24] 马永山,吴俊森,贾瑞宝,等.导数同步荧光法测定绿藻叶绿素a浓度[J].中国环境监测,2012,28(2):58-61.
[25] Mantoura R F C., Llewellyn C.A.. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography[J]. Anal.chim.Actu, 1983, 151:197-314.
[26] Wright S W, Jeffrey s w, Mantoura R F, et.al.. Improved HPLC method for the analysis of chlorophulls and carotenoids from marine phytoplankton[J]. Mar. Ecol.Progr. Series, 1991,77:183-196.
[27] 侯燕松,梁威,张丽萍,等.基于光合色素的化学分类法在淡水藻类研究中的应用[J].长江流域资源与环境. 2009, 18(6): 563-567.
[28] 程红艳,陈军辉,张道来,等. 超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b[J].海洋科学,2010, 34(2): 23-27.
[29] 潘德炉.利用荧光高度遥感海洋中叶绿素 a 的浓度[J]. 海洋学报, 1989,11(6):780-787.
[30] 刘建萍,张玉超,钱新. 遥感技术在湖泊叶绿素a监测中的应用研究[J]. 环境监测与预警, 2009,(12):33-36.
[31] 韦玉春,王国祥,程春梅,等.面向浑浊水体叶绿素a浓度遥感反演的光谱基线校正[J]. 光谱学与光谱分析, 2012, 32(9): 2546-2550.
[32] 夏晓芸,解启蒙,杨国范,等.大伙房水库叶绿素a浓度反演模型研究, 2018,(8): 39-42.
[33] 张明慧,苏华,季博文,等. MODIS时序影像的福建近岸叶绿素a浓度反演[J]. 环境科学学报, 2018,38(12):4831-4839.
[2] 万修志. 提取测定淡水藻中叶绿素a的方法研究[D].长春:山东建筑大学,2008.
[3] 刘建华. 荧光法测定水体中叶绿素含量影响因素的研究[D].石家庄:河北科技大学,2014.
[4] 国家环境保护总局. 水和废水监测分析方法(第4版)[M].北京: 中国环境科学出版社,2002: 670-672.
[5] 汪志国,王静,李国刚. 双波长分光广度法同时测定叶绿素[J]. 中国环境检测, 1999, 15(5):21-22.
[6] 韩桂春,谷丰,张忠臣. 淡水中叶绿素a测定方法的探讨[J].中国环境监测. 2005, 21(1): 55-57.
[7] 冯菁,李艳波,朱擎. 浮游植物叶绿素a测定方法比较[J]. 生态环境, 2008, 17(2): 524-527.
[8] 童桂凤,陈志芳,范莹.分光光度法测定叶绿素a的比较[J].环境监测管理与技术. 2012, 24(1): 53-55.
[9] 李会玲,赵锋,豆艳霞. 水体中叶绿素a测定方法的探究[J]. 分析测试,2017,48(3): 52-54.
[10] 王海黎,洪华生,徐立. 反相高效液相色谱法分离、测定海洋浮游植物的叶绿素和类胡萝卜素[J]. 海洋科学, 1999,(4): 6-9.
[11] 林少君,贺立静,黄沛生等. 浮游植物中叶绿素a提取方法的比较研究[J].生态科学, 2005, 24(1): 9-11.
[12] C. S.Yentsch, C. M. Yentsch. Fluorescence spectral Signatures: The Characterization of Phytoplankton Populations by Theuse of Excitation and Emission Spectra[J]. Mar. Res, 1979,37:471-483.
[13] Kaitala S, Babichenko S, Poryvkina L, Leebea A. Fluorescent analysis of pigment composition of natural phytoplankton[J]. Mar technol Soc J, 1994, 28: 50-58.
[14] Tsai-yun Lee, Mikio Tsuzuki. Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method[J]. Analytica Chimica Acta 1995,302: 81-87.
[15] D.Mckee,A. Cunningham, K. Jones. An integrated submersible Fluorometer/Nephelometer/Transmissometer:Design and Testing at Sea. Optics&Laser Technology, 1997,29(1):35-39.
[16] M. Beutler, K. H. Wiltshire, B. Meyer et al. A fluorometric Method for the Differentiation of Algal Populations in vivo and in situ. Photosynthesis Research, 2002,72(1):39-53.
[17] 金海龙. 基于荧光机理的海藻识别方法与实验研究[D].河北:燕山大学,2005.
[18] Bianchi,T.S. A comparion between fluorometric and high-performance liquid chromatography measurement[J]. Bulletin of marine science, 1995, 56(1):28-31.
[19] 王荣,鲁北纬,余家栋. 海水叶绿素a含量的连续走航测定[J]. 海洋与湖沼,1996,27(3):296-301.
[20] 郑龙江.光纤式海藻叶绿素a浓度的荧光测量理论与实验研究 [D].秦皇岛:燕山大学,2001.
[21] 唐尧基,游文玮,陈莹,等.同步荧光法测定海水中叶绿素 a 的含量[J].分析仪器,2004,(3):24-26.
[22] J.Gregor, B.Marsalek. Freshwater phytoplankton quantification by chlorophyll a: a comparative study of in vitro, in vivo and in situ methods[J]. Water Research. 2004,(38):517-522.
[23] 王刚,秦少平,晏光辉. LED 激发叶绿素荧光探测仪的设计研究[J].佳木斯大学学报(自然科学版),2010, 28(1): 39-41.
[24] 马永山,吴俊森,贾瑞宝,等.导数同步荧光法测定绿藻叶绿素a浓度[J].中国环境监测,2012,28(2):58-61.
[25] Mantoura R F C., Llewellyn C.A.. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography[J]. Anal.chim.Actu, 1983, 151:197-314.
[26] Wright S W, Jeffrey s w, Mantoura R F, et.al.. Improved HPLC method for the analysis of chlorophulls and carotenoids from marine phytoplankton[J]. Mar. Ecol.Progr. Series, 1991,77:183-196.
[27] 侯燕松,梁威,张丽萍,等.基于光合色素的化学分类法在淡水藻类研究中的应用[J].长江流域资源与环境. 2009, 18(6): 563-567.
[28] 程红艳,陈军辉,张道来,等. 超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b[J].海洋科学,2010, 34(2): 23-27.
[29] 潘德炉.利用荧光高度遥感海洋中叶绿素 a 的浓度[J]. 海洋学报, 1989,11(6):780-787.
[30] 刘建萍,张玉超,钱新. 遥感技术在湖泊叶绿素a监测中的应用研究[J]. 环境监测与预警, 2009,(12):33-36.
[31] 韦玉春,王国祥,程春梅,等.面向浑浊水体叶绿素a浓度遥感反演的光谱基线校正[J]. 光谱学与光谱分析, 2012, 32(9): 2546-2550.
[32] 夏晓芸,解启蒙,杨国范,等.大伙房水库叶绿素a浓度反演模型研究, 2018,(8): 39-42.
[33] 张明慧,苏华,季博文,等. MODIS时序影像的福建近岸叶绿素a浓度反演[J]. 环境科学学报, 2018,38(12):4831-4839.
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