烟草不同叶位多酚含量及相关酶活性变化的研究
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摘要
为分析烟叶在生长过程中的次生代谢物质动态变化特征、相关酶的活性变化规律,以3个烤烟种植试验点的烤烟为研究对象,研究不同叶位烟草叶片多酚类物质含量、抗氧化活性和表达量。结果表明,同一个采样点中,不同叶位烟叶多酚类物质含量表现为上叶位>中叶位>下叶位。不同叶位烟草叶片PAL、PPO、C4H和4CL活性以上叶位最高。通过多酚体外抗氧化测定,表明不同叶位多酚对DPPH和羟基自由基有一定的清除能力。荧光定量PCR检测发现,PAL基因和PPO基因在上叶位中的表达显著高于其他叶位。烤烟生长过程中的次生代谢物质动态变化,相关酶的活性变化及基因表达水平与烟叶成熟度存在一定的关联。
Aiming to analyze the dynamic changes of secondary metabolites and related enzymes during tobacco growth, the content, antioxidant activity and expression level of polyphenols in tobacco leaves at different leaf positions were studied in three tobacco planting experimental sites. In the same sampling site, the content of polyphenols in tobacco leaves of different leaf positions showed as follows:upper leaf position>middle leaf position>lower leaf position. The activities of PAL, PPO, C4 H and 4 CL in tobacco leaves at different positions showed that the enzyme activity in the upper leaves was the highest. The antioxidant activity of polyphenols in vitro showed that polyphenols in different leaf positions had certain scavenging ability to DPPH and hydroxyl free radicals. q-PCR analysis showed that the expression of PAL and PPO genes in the upper leaf was significantly higher than that of other leaf positions. Dynamic changes of secondary metabolites, enzyme activity and gene expression levels were correlated with tobacco maturity during the growth of flue-cured tobacco.
Aiming to analyze the dynamic changes of secondary metabolites and related enzymes during tobacco growth, the content, antioxidant activity and expression level of polyphenols in tobacco leaves at different leaf positions were studied in three tobacco planting experimental sites. In the same sampling site, the content of polyphenols in tobacco leaves of different leaf positions showed as follows:upper leaf position>middle leaf position>lower leaf position. The activities of PAL, PPO, C4 H and 4 CL in tobacco leaves at different positions showed that the enzyme activity in the upper leaves was the highest. The antioxidant activity of polyphenols in vitro showed that polyphenols in different leaf positions had certain scavenging ability to DPPH and hydroxyl free radicals. q-PCR analysis showed that the expression of PAL and PPO genes in the upper leaf was significantly higher than that of other leaf positions. Dynamic changes of secondary metabolites, enzyme activity and gene expression levels were correlated with tobacco maturity during the growth of flue-cured tobacco.
引文
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[14]吴林秀,胡荣康,雷雅婷,等.灰树花液态发酵菌丝体工艺优化及其体外抗氧化活性[J].食品工业科技,2018,39(13):187-191,197.
[15]孟黎明,张丽英,黄广华,等.不同采收成熟度对红花大金元烟叶品质的影响[J].西南农业学报,2015,28(2):853-856.
[16]刘阳,高丽君,蔡宪杰,等.采收成熟度对烤烟多酚含量和组成的影响[J].烟草科技,2011(8):73-78.
[17]王丹,杨虹琦,岳骞,等.烤烟成熟期多酚及相关氧化酶活性的变化[J].湖南农业大学学报(自然科学版),2008,34(5):538-540.
[18]孟莉,郭世英,符云鹏,等.吉林晒烟多酚类物质及其相关酶活性的变化动态[J].中国烟草科学,2014,35(6):59-64.
[19]袁卫瑜,王兴德,李军营,等.烤烟生长过程中多酚类物质及其相关酶活性的变化[J].西南农业学报,2017,30(7):1528-1532.
[20]杨银菊,张彦,王树声,等.打顶对烟草叶片多酚代谢及其关键酶的影响[J].中国烟草学报,2018,24(1):60-67.
[21]ZHAO J,HAN J,ZHANG J,et al.NtPHYB1K326,a homologous gene of Arabidopsis PHYB,positively regulates the content of phenolic compounds in tobacco[J].Plant physiology&biochemistry,2016,109:45-53.
[2]闫克玉.烟草化学[M].郑州:郑州大学出版社,2002.
[3]李力,杨涓,戴亚,等.烤烟中绿原酸、莨菪亭和芸香苷的分布研究[J].中国烟草学报,2008,14(4):13-17.
[4]宗浩.云南大理特色优质烤烟品质差异化及区划研究[D].北京:中国农业科学院,2012.
[5]YUAN C,QI L,MA F F,et al.Identification and characterization of differentially expressed genes from tobacco roots after decapitation[J].Acta Physiologiae Plantarum,2012,34(2):479-493.
[6]CL魪C,HILL L M,NIGGEWEG R,et al.Modulation of chlorogenic acid biosynthesis in Solanum lycopersicum;consequences for phenolic accumulation and UV-tolerance[J].Phytochemistry,2008,69(11):2149-2156.
[7]ZUCKER M,NITHSCH C,NITHSCH J P.The induction of flowering in nicotiana:Photoperiodic alternation of the chlorogenic acid concentration[J].Amer J Bot,1985,52:271-277.
[8]叶金果.典型浓香型烤烟主要次生代谢物质及其与品质关系研究[D].郑州:河南农业大学,2012.
[9]万诚,刘仁祥,聂琼,等.不同烟草品种绿原酸含量变化研究[J].山地农业生物学报,2016,35(2):25-28.
[10]韩锦峰.烟草栽培生理[M].北京:中国农业出版社,2003.
[11]赵铭钦,陈红华,陈秋会,等.不同陈化条件下烤烟多酚类物质的动态变化及其与化学成分的相关分析[J].中国烟草学报,2007,13(4):16-18.
[12]KONG D X,LI Y Q,BAI M,et al.Correlation between the dynamic accumulation of the main effective components and their associated regulatory enzyme activities at different growth stages in Lonicera japonica Thunb[J].Industrial crops&products,2017,96:16-22.
[13]李西柳,庞明,王俊儒,等.柿子渣中多酚的提取工艺及其抗氧化性研究[J].西北植物学报,2010,30(7):1475-1480.
[14]吴林秀,胡荣康,雷雅婷,等.灰树花液态发酵菌丝体工艺优化及其体外抗氧化活性[J].食品工业科技,2018,39(13):187-191,197.
[15]孟黎明,张丽英,黄广华,等.不同采收成熟度对红花大金元烟叶品质的影响[J].西南农业学报,2015,28(2):853-856.
[16]刘阳,高丽君,蔡宪杰,等.采收成熟度对烤烟多酚含量和组成的影响[J].烟草科技,2011(8):73-78.
[17]王丹,杨虹琦,岳骞,等.烤烟成熟期多酚及相关氧化酶活性的变化[J].湖南农业大学学报(自然科学版),2008,34(5):538-540.
[18]孟莉,郭世英,符云鹏,等.吉林晒烟多酚类物质及其相关酶活性的变化动态[J].中国烟草科学,2014,35(6):59-64.
[19]袁卫瑜,王兴德,李军营,等.烤烟生长过程中多酚类物质及其相关酶活性的变化[J].西南农业学报,2017,30(7):1528-1532.
[20]杨银菊,张彦,王树声,等.打顶对烟草叶片多酚代谢及其关键酶的影响[J].中国烟草学报,2018,24(1):60-67.
[21]ZHAO J,HAN J,ZHANG J,et al.NtPHYB1K326,a homologous gene of Arabidopsis PHYB,positively regulates the content of phenolic compounds in tobacco[J].Plant physiology&biochemistry,2016,109:45-53.
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