温度、照度和氮磷营养盐对长茎葡萄蕨藻富硒的影响
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摘要
【目的】探索富硒长茎葡萄蕨藻(Caulerpalentillifera)的培养条件。【方法】以亚硒酸钠(Na_2SeO_3)为硒源,采用单因素实验,研究不同硒浓度对长茎葡萄蕨藻的生长、光合色素和硒富集量的影响,通过正交实验,找出适宜长茎葡萄蕨藻富硒的最佳温度、照度和氮磷比组合条件。【结果】在亚硒酸钠0、0.5、1.0、2.0、4.0、8.0mg/L条件下,随亚硒酸钠添加量增加,藻体硒质量分数(干基)从1.70μg/g增加至50.10μg/g,且各组间差异均有统计学意义(P<0.05);亚硒酸钠添加量2.0 mg/L时,藻体硒质量分数达到11.7μg/g;添加量大于2.0 mg/L时,显著抑制藻体的生长(P<0.05),藻体叶绿素a和叶绿素b含量显著性降低(P<0.05);添加量低于2.0 mg/L时,藻体的生长、叶绿素a和叶绿素b含量均无显著性差异;类胡萝卜素含量各组间无明显变化。在培养液亚硒酸钠质量浓度为2.0 mg/L的条件下,藻体最适的生长条件组合是温度28℃、照度2 000 lx、氮磷比10∶1,藻体的最优富硒条件是温度24℃、照度2 000 lx、氮磷比8∶1。【结论】长茎葡萄蕨藻在开发含硒安全性功能海藻食品方面有较大应用潜力。
To explore the culture conditions of high value-added selenium-enriched Caulerpa lentillifera.【Method】Using sodium selenite(Na_2 Se O_3) as the selenium source, the effects of different selenium concentrations on the growth, photosynthetic pigments and selenium enrichment of C. lentillifera were studied by single factor experiment. The orthogonal experiment was designed to find the optimum conditions for the temperature, illuminance and N/P ratio of selenium-enriched C. lentillifera. 【Result】 The sodium selenite in culture solution was in the range of 0 to 8.0 mg/L. With the increase of selenium concentration, the selenium contents of algae(dry weight) increased from 1.70 μg/g to 50.10 μg/g.There was a significant difference among the groups(P < 0.05). When the content of sodium selenite was 2.0 mg/L, the selenium contents of algae reached 11.7 μg/g. When selenium concentration was higher than 2.0 mg/L, the growth of algae was significantly inhibited(P < 0.05), and the contents of chlorophyll a and chlorophyll b were significantly decreased(P < 0.05). When selenium concentration was less than 2.0 mg/L, there was no significant difference in the growth, and contents of chlorophyll a and chlorophyll b. There was no significant change in carotenoid content among the groups. The optimal combination for cultivating C. lentillifera was when selenium concentration was 2.0 mg/L, the temperature was 28 ℃, the illumination was 2 000 lx and the N/P ratio of 10∶1. The optimal selenium-rich conditions for algae were as follows: temperature 24 ℃, illumination 2 000 lx and N/P ratios 8∶1.【Conclusion】C. lentillifera has great potential in the development of seaweed foods containing selenium safe functions.
To explore the culture conditions of high value-added selenium-enriched Caulerpa lentillifera.【Method】Using sodium selenite(Na_2 Se O_3) as the selenium source, the effects of different selenium concentrations on the growth, photosynthetic pigments and selenium enrichment of C. lentillifera were studied by single factor experiment. The orthogonal experiment was designed to find the optimum conditions for the temperature, illuminance and N/P ratio of selenium-enriched C. lentillifera. 【Result】 The sodium selenite in culture solution was in the range of 0 to 8.0 mg/L. With the increase of selenium concentration, the selenium contents of algae(dry weight) increased from 1.70 μg/g to 50.10 μg/g.There was a significant difference among the groups(P < 0.05). When the content of sodium selenite was 2.0 mg/L, the selenium contents of algae reached 11.7 μg/g. When selenium concentration was higher than 2.0 mg/L, the growth of algae was significantly inhibited(P < 0.05), and the contents of chlorophyll a and chlorophyll b were significantly decreased(P < 0.05). When selenium concentration was less than 2.0 mg/L, there was no significant difference in the growth, and contents of chlorophyll a and chlorophyll b. There was no significant change in carotenoid content among the groups. The optimal combination for cultivating C. lentillifera was when selenium concentration was 2.0 mg/L, the temperature was 28 ℃, the illumination was 2 000 lx and the N/P ratio of 10∶1. The optimal selenium-rich conditions for algae were as follows: temperature 24 ℃, illumination 2 000 lx and N/P ratios 8∶1.【Conclusion】C. lentillifera has great potential in the development of seaweed foods containing selenium safe functions.
引文
[1]王秀红.微量元素硒与人体健康[J].微量元素与健康研究,2006,23(1):66-67.
[2]黄建军.浅评《植物有机硒的化学及其医学应用》[J].生态科学,2002,21(2):191-191.
[3]侯勤堂,刘琼.藻类硒富集与硒蛋白研究进展[J].微量元素与健康研究,2010,27(2):61-66.
[4]朱善良.硒的生物学作用及其研究进展[J].生物学通报,2004,39(6):6-8.
[5]王永会,周大迈,张爱军,等.外源硒对谷子抗氧化酶活性及其品质的影响[J].中国土壤与肥料,2015(4):112-117.
[6]熊咏民,杨晓莉,等.硒的生物学效应与环境相关性疾病的研究进展[J].土壤2018,50(6):1105-1112.
[7]程义勇.《中国居民膳食营养素参考摄入量》2013修订版简介[J].营养学报,2014,36(4):313-317.
[8]唐新欣,贺蓉.中国缺硒状况的调查[J].医药世界,2002(6):22-24.
[9]ADADI P,BARAKOVA N V,MURAVYOV K Y,et al.Designing selenium functional foods and beverages:Areview[J/OL].Food Research International,2018-11-17.https://doi.org/10.1016/j.foodres.2018.11.029
[10]孙显,杨宇峰.富硒藻类研究进展[J].生态毒理学报,2016,11(2):101-110.
[11]SCHIAVON M,PILON-SMITS E A.The fascinating facets of plant selenium accumulation-biochemistry,physiology,evolution and ecology[J].New Phytologist,2017,213:1582-1596
[12]钟钰.富硒裂片石莼的生理生化特征及其抗肿瘤活性研究[D].广州:暨南大学,2014.
[13]LIU Z W,WANG Q,ZOU D H,et al.Effects of selenite on growth,photosynthesis and antioxidant system in seaweeds,Ulva fasciata(Chlorophyta)and Gracilaria lemaneiformis(Rhodophyta)[J].Algal Research,2018,36:115-124.
[14]PAUL N A,NEVEUX N,MAGNUSSON M,et al.Comparative production and nutritional value of“sea grapes”―the tropical green seaweeds Caulerpa lentillifera and C.racemosa[J].Journal of Applied Phycology,2014,26(4):1833-1844.
[15]DE GAILLANDE C,PAYRI C,REMOISSENET G,et al.Caulerpa consumption,nutritional value and farming in the Indo-Pacific region[J].Journal of Applied Phycology,2017,29(5):2249-2266.
[16]SHARMA B R,KIM H J,RHYU D Y.Caulerpa lentilliferainhibits protein-tyrosine phosphatase 1B and protects pancreatic beta cell via its insulin mimetic effect[J].Food Science and Biotechnology,2017,26(2):495-499.
[17]郭辉.海葡萄(Caulerpa lentillifera)切断组织再生培养及发育条件研究[D].青岛:中国科学院研究生院(海洋研究所),2014.
[18]周文川,赵秋龙,雷美华,等.光照等环境因子对长茎葡萄蕨藻生长的影响[J].海洋与渔业,2017(6):70-72.
[19]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-137.
[20]杨逸萍,胡明辉.海洋浮游植物对硒的摄取和转化[J].应用海洋学学报,1996(4):319-323.
[21]吕蓉,张波.硒浓度对小球藻生长生物富集的影响[J].盐业与化工,2008(4):35-37.
[22]ZHENG Y,LI Z,TAO M,et al.Effects of selenite on green microalga Haematococcus pluvialis:Bioaccumulation of selenium and enhancement of astaxanthin production[J].Aquatic Toxicology,2017,183:21-27.
[23]SCHIAVON M,ERTANI A,PARRASIA S,et al.Selenium accumulation and metabolism in algae[J].Aquatic Toxicology,2017,189:1-8.
[24]刘静雯,张稚兰,杜翠红,等.富硒海洋球石藻(Emiliania huxleyi)的培养及其硒蛋白的初步分离纯化[J].海洋与湖沼,2010,41(6):879-884.
[25]郑立,严小军,孙建,等.日本真海带对硒的积累和生物转化[J].海洋学报,2005,27(5):121-127.
[26]黄峰,单雪风,张水妹,等.螺旋藻富硒转化含硒蛋白质的鉴定及其抗氧化活性研究[J].现代食品科技,2015(7):99-104.
[27]唐贤明,刘小霞,孟凡同,等.海马齿和长茎葡萄蕨藻的营养成分分析及评价[J].热带生物学报,2018,9(2):129-135.
[28]GOJKOVIC?,GARBAYO I,ARIZA J L G,et al.Selenium bioaccumulation and toxicity in cultures of green microalgae[J].Algal Research,2015,7(7):106-116.
[29]王洪斌,成明,李士虎,等.4种海洋微藻对外源硒的吸收和胁迫的响应[J].水产科学,2013,32(5):289-292.
[30]周志刚,钟罡,刘志礼.硒对极大螺旋藻生长及含硒量的影响[J].海洋科学,1997,21(5):42-45.
[31]杨芳,郑文杰,唐全民,等.Se(Ⅳ)对钝顶和极大螺旋藻生长的影响[J].暨南大学学报(自然科学与医学版),2005,26(5):678-683.
[32]郑文杰,贺鸿志,黄峙,等.螺旋藻富集和转化硒研究进展[J].中国生物工程杂志,2003,23(1):57-60.
[33]ZHOU Z G,ZHONG G,LIU Z L.Effects of selenium on the growth and selenium contents of Spirulina maxima[J].Marineences,1997,5:42-45.
[34]高海洋.LED光照对富锌和富硒螺旋藻生长的影响[D].湛江:广东海洋大学,2016.
[35]WANG W X,DEI R C H.Influences of phosphate and silicate on Cr(VI)and Se(IV)accumulation in marine phytoplankton[J].Aquatic Toxicology,2001,52(1):39-47.
[36]WANG W X,DEI R C H.Effects of major nutrient additions on metal uptake in phytoplankton[J].Environmental Pollution,2001,111(2):233-240.
[37]邢丹英.作物富硒技术研究与应用[D].武汉:华中农业大学,2004.
[38]田秀英,李会合,王正银.施硒对苦荞N,P,K营养元素和土壤有效养分含量的影响[J].水土保持学报,2009,23(3):112-115.
[39]肖利杰,刘春梅,李梅,等.施硒对水稻硒·氮·磷·钾含量及产量的影响[J].安徽农业科学,2015,503(34):59-61.
[40]CHAI Ying,ZHAO Jing,HUANG Ting,et al.Effects of different combination ratios of N,P fertilizer on nutrient uptake of Maize and Yield[J].Xinjiang Agricultural Sciences,2015,52(3):444-449;454.
[41]JIA Zhao-dong,MA Pei-yong,BIAN Xiao-feng,et al.Effects of different phosphorus application rates on dry matter accumulation and N,P,K absorption and utilization in Sweetpotato[J].Southwest China Journal of Agricultural Sciences,2016,29(6):1358-1365.
[42]ZHU Dan,LIU Fang,WU San-lin,et al.The influence of different application rate of N,P and K on the yield of Paeonia stia[J].Journal of Leshan Normal University,2016,31(12):43-48.
[2]黄建军.浅评《植物有机硒的化学及其医学应用》[J].生态科学,2002,21(2):191-191.
[3]侯勤堂,刘琼.藻类硒富集与硒蛋白研究进展[J].微量元素与健康研究,2010,27(2):61-66.
[4]朱善良.硒的生物学作用及其研究进展[J].生物学通报,2004,39(6):6-8.
[5]王永会,周大迈,张爱军,等.外源硒对谷子抗氧化酶活性及其品质的影响[J].中国土壤与肥料,2015(4):112-117.
[6]熊咏民,杨晓莉,等.硒的生物学效应与环境相关性疾病的研究进展[J].土壤2018,50(6):1105-1112.
[7]程义勇.《中国居民膳食营养素参考摄入量》2013修订版简介[J].营养学报,2014,36(4):313-317.
[8]唐新欣,贺蓉.中国缺硒状况的调查[J].医药世界,2002(6):22-24.
[9]ADADI P,BARAKOVA N V,MURAVYOV K Y,et al.Designing selenium functional foods and beverages:Areview[J/OL].Food Research International,2018-11-17.https://doi.org/10.1016/j.foodres.2018.11.029
[10]孙显,杨宇峰.富硒藻类研究进展[J].生态毒理学报,2016,11(2):101-110.
[11]SCHIAVON M,PILON-SMITS E A.The fascinating facets of plant selenium accumulation-biochemistry,physiology,evolution and ecology[J].New Phytologist,2017,213:1582-1596
[12]钟钰.富硒裂片石莼的生理生化特征及其抗肿瘤活性研究[D].广州:暨南大学,2014.
[13]LIU Z W,WANG Q,ZOU D H,et al.Effects of selenite on growth,photosynthesis and antioxidant system in seaweeds,Ulva fasciata(Chlorophyta)and Gracilaria lemaneiformis(Rhodophyta)[J].Algal Research,2018,36:115-124.
[14]PAUL N A,NEVEUX N,MAGNUSSON M,et al.Comparative production and nutritional value of“sea grapes”―the tropical green seaweeds Caulerpa lentillifera and C.racemosa[J].Journal of Applied Phycology,2014,26(4):1833-1844.
[15]DE GAILLANDE C,PAYRI C,REMOISSENET G,et al.Caulerpa consumption,nutritional value and farming in the Indo-Pacific region[J].Journal of Applied Phycology,2017,29(5):2249-2266.
[16]SHARMA B R,KIM H J,RHYU D Y.Caulerpa lentilliferainhibits protein-tyrosine phosphatase 1B and protects pancreatic beta cell via its insulin mimetic effect[J].Food Science and Biotechnology,2017,26(2):495-499.
[17]郭辉.海葡萄(Caulerpa lentillifera)切断组织再生培养及发育条件研究[D].青岛:中国科学院研究生院(海洋研究所),2014.
[18]周文川,赵秋龙,雷美华,等.光照等环境因子对长茎葡萄蕨藻生长的影响[J].海洋与渔业,2017(6):70-72.
[19]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-137.
[20]杨逸萍,胡明辉.海洋浮游植物对硒的摄取和转化[J].应用海洋学学报,1996(4):319-323.
[21]吕蓉,张波.硒浓度对小球藻生长生物富集的影响[J].盐业与化工,2008(4):35-37.
[22]ZHENG Y,LI Z,TAO M,et al.Effects of selenite on green microalga Haematococcus pluvialis:Bioaccumulation of selenium and enhancement of astaxanthin production[J].Aquatic Toxicology,2017,183:21-27.
[23]SCHIAVON M,ERTANI A,PARRASIA S,et al.Selenium accumulation and metabolism in algae[J].Aquatic Toxicology,2017,189:1-8.
[24]刘静雯,张稚兰,杜翠红,等.富硒海洋球石藻(Emiliania huxleyi)的培养及其硒蛋白的初步分离纯化[J].海洋与湖沼,2010,41(6):879-884.
[25]郑立,严小军,孙建,等.日本真海带对硒的积累和生物转化[J].海洋学报,2005,27(5):121-127.
[26]黄峰,单雪风,张水妹,等.螺旋藻富硒转化含硒蛋白质的鉴定及其抗氧化活性研究[J].现代食品科技,2015(7):99-104.
[27]唐贤明,刘小霞,孟凡同,等.海马齿和长茎葡萄蕨藻的营养成分分析及评价[J].热带生物学报,2018,9(2):129-135.
[28]GOJKOVIC?,GARBAYO I,ARIZA J L G,et al.Selenium bioaccumulation and toxicity in cultures of green microalgae[J].Algal Research,2015,7(7):106-116.
[29]王洪斌,成明,李士虎,等.4种海洋微藻对外源硒的吸收和胁迫的响应[J].水产科学,2013,32(5):289-292.
[30]周志刚,钟罡,刘志礼.硒对极大螺旋藻生长及含硒量的影响[J].海洋科学,1997,21(5):42-45.
[31]杨芳,郑文杰,唐全民,等.Se(Ⅳ)对钝顶和极大螺旋藻生长的影响[J].暨南大学学报(自然科学与医学版),2005,26(5):678-683.
[32]郑文杰,贺鸿志,黄峙,等.螺旋藻富集和转化硒研究进展[J].中国生物工程杂志,2003,23(1):57-60.
[33]ZHOU Z G,ZHONG G,LIU Z L.Effects of selenium on the growth and selenium contents of Spirulina maxima[J].Marineences,1997,5:42-45.
[34]高海洋.LED光照对富锌和富硒螺旋藻生长的影响[D].湛江:广东海洋大学,2016.
[35]WANG W X,DEI R C H.Influences of phosphate and silicate on Cr(VI)and Se(IV)accumulation in marine phytoplankton[J].Aquatic Toxicology,2001,52(1):39-47.
[36]WANG W X,DEI R C H.Effects of major nutrient additions on metal uptake in phytoplankton[J].Environmental Pollution,2001,111(2):233-240.
[37]邢丹英.作物富硒技术研究与应用[D].武汉:华中农业大学,2004.
[38]田秀英,李会合,王正银.施硒对苦荞N,P,K营养元素和土壤有效养分含量的影响[J].水土保持学报,2009,23(3):112-115.
[39]肖利杰,刘春梅,李梅,等.施硒对水稻硒·氮·磷·钾含量及产量的影响[J].安徽农业科学,2015,503(34):59-61.
[40]CHAI Ying,ZHAO Jing,HUANG Ting,et al.Effects of different combination ratios of N,P fertilizer on nutrient uptake of Maize and Yield[J].Xinjiang Agricultural Sciences,2015,52(3):444-449;454.
[41]JIA Zhao-dong,MA Pei-yong,BIAN Xiao-feng,et al.Effects of different phosphorus application rates on dry matter accumulation and N,P,K absorption and utilization in Sweetpotato[J].Southwest China Journal of Agricultural Sciences,2016,29(6):1358-1365.
[42]ZHU Dan,LIU Fang,WU San-lin,et al.The influence of different application rate of N,P and K on the yield of Paeonia stia[J].Journal of Leshan Normal University,2016,31(12):43-48.
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