生物膜干涉技术在生物分子间相互作用检测中的应用
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摘要
生物膜干涉技术可以实现生物分子之间相互作用的实时分析检测,广泛应用于高校科研实验室的药物开发过程,是一种非标记的、实时监测的先进技术。本文结合生物分子相互作用分析系统的技术原理和实际使用情况,详尽叙述了其在生物分子间相互作用检测的方法和应用,旨在为相关领域的科研工作者提供实验帮助和参考。
Octet biomolecule interaction analysis system is a multi-functional, label-free, real-time analysis instrument. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity, and screening proteinprotein and protein-small molecule interactions. In this paper, based on the technical principle and practical application of biomolecular interaction analysis system, the method and application of biomolecular interaction detection system are described in detail, aiming at providing experimental help and reference for researchers in related fields.
Octet biomolecule interaction analysis system is a multi-functional, label-free, real-time analysis instrument. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity, and screening proteinprotein and protein-small molecule interactions. In this paper, based on the technical principle and practical application of biomolecular interaction analysis system, the method and application of biomolecular interaction detection system are described in detail, aiming at providing experimental help and reference for researchers in related fields.
引文
[1]尹伟,肖桂凤,刘双双,等.构建一流公共仪器平台的关键与对策[J].实验室研究与探索,2015,(06)273-276.
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[5]王静.生物分子相互作用分析技术在药物研究领域的应用.第十一届中国生命科学公共平台管理与发展研讨会.2018.中国江苏南京.
[6]杨国泰,吴鑫,李福来,等.生物膜层干涉技术在生物分子分析和检测中应用的研究进展[J].分析测试学报,2017,36(08):1055-1060.
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[8]Concepcion J,Witte K,Wartchow C,et al Label-Free Detection of Biomolecular Interactions Using Bio Layer Interferometry for Kinetic Characterization[J].Combinatorial Chemistry&High Throughput Screening,2009,12(8):791-800.
[9]张良林,王帅,高宏.应用生物膜干涉技术检测鱼腥草Het R结合靶DNA的亲和力,水生生物学报,2017,41(2):483-485.
[10]于怡.碳基纳米材料与生物分子相互作用的分子动力学研究.2018,苏州大学.
[11]https://www.fortebio.com/octet-RED96.html.
[12]Han B,Zhang M,Sun P,et al.Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay[J].Jove-Journal of Visualized Experiments,2018,133.
[13]张晟,罗弟祥,刘怡,等.生物膜干涉技术、HPLC及ELISA在抗体定量检测中的比较分析[J].免疫学杂志,2014,30(4):338-341.
[14]Ren B B,Sayed AMM,Tan H L,et al.Identifying Protein Interactions with Histone Peptides Using Biolayer Interferometry[J].Bio-protocol,2018,8(18)288-302.
[15]Kamat V,Rafique A.Designing binding kinetic assay on the bio-layer interferometry(BLI)biosensor to characterize antibody-antigen interactions[J]Analytical Biochemistry,2017,536:16-31.
[16]Kumaraswamy S,Tobias R.Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry[J].Methods in molecular biology(Clifton,N.J.),2015,1278:165-182.
[17]Wang Y R,Carta G.Rapid and Sensitive Detection of the Interaction of Human Papillomavirus Virus-Like Particles with Yeast Whole Cell RNA Using Biolayer Interferometry[J].Biotechnology Journal,2019,14(1):1800303.
[18]Petersen R L.Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development[R].Biosensors-basel,2017,7(4):E49.
[19]Sanders M,McPartlin D,Moran K,et al.Comparison of Enzyme-Linked Immunosorbent Assay,Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust[J].Toxins(Basel),2016,8(4):103.
[20]Li J,Schantz A,Schwegler M,et al.Detection of low-affinity anti-drug antibodies and improved drug tolerance in immunogenicity testing by Octet biolayer interferometry[J].J Pharm Biomed Anal,2011,54(2):286-94.
[21]Abdiche Y,Malashock D,Pinkerton A,et al.Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor,the Octet[J].Analytical Biochemistry,2008,377(2):209-217.
[2]姜丽艳,张艳,张爱军,等.蛋白质组学分析仪器在本科实验教学中的应用与实践[J].中国教育技术装备,2015,(22):153-155.
[3]姜丽艳,张艳,李帅,等.生命科学公共技术共享平台运行管理模式的探索[J].中国现代教育装备2015,(03):1-4.
[4]Kamat V,Rafique A.Designing binding kinetic assay on the bio-layer interferometry(BLI)biosensor to characterize antibody-antigen interactions[J]Analytical Biochemistry,2017,536:16-31.
[5]王静.生物分子相互作用分析技术在药物研究领域的应用.第十一届中国生命科学公共平台管理与发展研讨会.2018.中国江苏南京.
[6]杨国泰,吴鑫,李福来,等.生物膜层干涉技术在生物分子分析和检测中应用的研究进展[J].分析测试学报,2017,36(08):1055-1060.
[7]顾宗林.纳米材料与重要生物分子的相互作用机理及生物效应研究.2018,苏州大学.
[8]Concepcion J,Witte K,Wartchow C,et al Label-Free Detection of Biomolecular Interactions Using Bio Layer Interferometry for Kinetic Characterization[J].Combinatorial Chemistry&High Throughput Screening,2009,12(8):791-800.
[9]张良林,王帅,高宏.应用生物膜干涉技术检测鱼腥草Het R结合靶DNA的亲和力,水生生物学报,2017,41(2):483-485.
[10]于怡.碳基纳米材料与生物分子相互作用的分子动力学研究.2018,苏州大学.
[11]https://www.fortebio.com/octet-RED96.html.
[12]Han B,Zhang M,Sun P,et al.Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay[J].Jove-Journal of Visualized Experiments,2018,133.
[13]张晟,罗弟祥,刘怡,等.生物膜干涉技术、HPLC及ELISA在抗体定量检测中的比较分析[J].免疫学杂志,2014,30(4):338-341.
[14]Ren B B,Sayed AMM,Tan H L,et al.Identifying Protein Interactions with Histone Peptides Using Biolayer Interferometry[J].Bio-protocol,2018,8(18)288-302.
[15]Kamat V,Rafique A.Designing binding kinetic assay on the bio-layer interferometry(BLI)biosensor to characterize antibody-antigen interactions[J]Analytical Biochemistry,2017,536:16-31.
[16]Kumaraswamy S,Tobias R.Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry[J].Methods in molecular biology(Clifton,N.J.),2015,1278:165-182.
[17]Wang Y R,Carta G.Rapid and Sensitive Detection of the Interaction of Human Papillomavirus Virus-Like Particles with Yeast Whole Cell RNA Using Biolayer Interferometry[J].Biotechnology Journal,2019,14(1):1800303.
[18]Petersen R L.Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development[R].Biosensors-basel,2017,7(4):E49.
[19]Sanders M,McPartlin D,Moran K,et al.Comparison of Enzyme-Linked Immunosorbent Assay,Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust[J].Toxins(Basel),2016,8(4):103.
[20]Li J,Schantz A,Schwegler M,et al.Detection of low-affinity anti-drug antibodies and improved drug tolerance in immunogenicity testing by Octet biolayer interferometry[J].J Pharm Biomed Anal,2011,54(2):286-94.
[21]Abdiche Y,Malashock D,Pinkerton A,et al.Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor,the Octet[J].Analytical Biochemistry,2008,377(2):209-217.
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