婴儿粪便中益生菌的分离筛选及培养
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摘要
采用从婴儿粪便中提取具有益生作用的菌种,为下一步的研究开发做乳酸菌种库做积累。选取河南省洛阳市5位志愿者进行样品采集,得到5份完全由母乳喂养的婴儿(2月龄)自然排出的粪便样,并分离纯化出5株乳酸菌(RS1, RS2,RS3,RS4,RS5)和5株双歧杆菌(SQ1,SQ2,SQ3,SQ4,SQ5),经鉴定,确定RS3和SQ1具有益生作用的。结果表明,此2株菌具有较好的耐酸性、胆盐耐受力,对温度敏感,对模拟胃液和肠液具有较强的耐受力,且对大肠杆菌、沙门氏菌具有95%以上的抑菌率,可作为优良的益生菌候选菌种。
Along with increased recognition of the probiotics, however, the basic mechanism of probiotics hasn't been completely unified up to now,which needs further research and analysis. The purpose of this study was to isolate probiotic strains from the feces of infants,and to lay the foundation for constructing the library of probiotics next step. Five natural excrement samples were collected from full breastfeeding babies(2 months) of Luoyang He'nan,five Lactic acid bacteria(RS1,RS2,RS3,RS4, RS5) and five Bifidobacterium(SQ1, SQ2, SQ3, SQ4, SQ5) were isolated after purification. Furthermore, two strains(RS3,SQ1) were characterized to have probiotic function. The results showed that these two strains were well resistant to acid and bile condition,sensitive to temperature,and highly tolerant to simulated gastric and intestinal fluid. In addition,up to 95% E. Coli and Salmonella were inhibited by these two strains. Based on their phenotypic characteristics,(RS3) and(SQ1) can be excellent candidates of probiotics library.
Along with increased recognition of the probiotics, however, the basic mechanism of probiotics hasn't been completely unified up to now,which needs further research and analysis. The purpose of this study was to isolate probiotic strains from the feces of infants,and to lay the foundation for constructing the library of probiotics next step. Five natural excrement samples were collected from full breastfeeding babies(2 months) of Luoyang He'nan,five Lactic acid bacteria(RS1,RS2,RS3,RS4, RS5) and five Bifidobacterium(SQ1, SQ2, SQ3, SQ4, SQ5) were isolated after purification. Furthermore, two strains(RS3,SQ1) were characterized to have probiotic function. The results showed that these two strains were well resistant to acid and bile condition,sensitive to temperature,and highly tolerant to simulated gastric and intestinal fluid. In addition,up to 95% E. Coli and Salmonella were inhibited by these two strains. Based on their phenotypic characteristics,(RS3) and(SQ1) can be excellent candidates of probiotics library.
引文
[1]魏向倩.婴儿生后粪便微生物区系的演变[D].石家庄:河北医科大学,2015.
[2]李梅花.中国部分地区人体粪便中双歧杆菌的分离鉴定及具潜在益生特性菌株的筛选[D].呼和浩特:内蒙古农业大学,2012.
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[4]徐营,李霞,杨利国.双歧杆菌的生物学特性及对人体的生理功能[J].微生物通报,2001(6):9 196.
[5]董银苹,于红霞,李凤琴.乳酸杆菌分离鉴定技术研究进展[J].卫生研究,2006,37(4):508-510.
[6]王永山,诸玉梅,朱小翠,等.5株乳酸菌的分离鉴定与生物学特性研究[J].江苏农业科学,2011(1):390-392.
[7]周家春,冯屏.双歧杆菌属的生理特征和鉴别[J].广州食品工业科技,2003(B11):59-62.
[8]丁伟亮,王光黄.乳酸菌分离鉴定概况[J].中国畜禽种业,2012(1):57-58.
[9]郭志杰,李鑫.猪源乳酸杆菌的分离、筛选、鉴定及产酸性能的研究[J].饲料工业,2010(20):45-48.
[10]李青青,傅伟,陈路清.耐氧性双歧杆菌Bifidobacterium animalis subsp.1actis Qq08的生物学特性研究[J].中国食品学报,2010,10(6):59-63.
[11]吕耀龙,赵春杰.双歧杆菌的代谢及开发前景[J].乳业科学与技术,2008(2):90-92.
[12]金京顺.双歧杆菌的特殊生理保健功能[J].上海医学,2005,28(3):245-248.
[2]李梅花.中国部分地区人体粪便中双歧杆菌的分离鉴定及具潜在益生特性菌株的筛选[D].呼和浩特:内蒙古农业大学,2012.
[3]赵红霞,詹勇,许梓荣.乳酸菌的研究及其应用[J].江西饲料,2003(1)):9-12.
[4]徐营,李霞,杨利国.双歧杆菌的生物学特性及对人体的生理功能[J].微生物通报,2001(6):9 196.
[5]董银苹,于红霞,李凤琴.乳酸杆菌分离鉴定技术研究进展[J].卫生研究,2006,37(4):508-510.
[6]王永山,诸玉梅,朱小翠,等.5株乳酸菌的分离鉴定与生物学特性研究[J].江苏农业科学,2011(1):390-392.
[7]周家春,冯屏.双歧杆菌属的生理特征和鉴别[J].广州食品工业科技,2003(B11):59-62.
[8]丁伟亮,王光黄.乳酸菌分离鉴定概况[J].中国畜禽种业,2012(1):57-58.
[9]郭志杰,李鑫.猪源乳酸杆菌的分离、筛选、鉴定及产酸性能的研究[J].饲料工业,2010(20):45-48.
[10]李青青,傅伟,陈路清.耐氧性双歧杆菌Bifidobacterium animalis subsp.1actis Qq08的生物学特性研究[J].中国食品学报,2010,10(6):59-63.
[11]吕耀龙,赵春杰.双歧杆菌的代谢及开发前景[J].乳业科学与技术,2008(2):90-92.
[12]金京顺.双歧杆菌的特殊生理保健功能[J].上海医学,2005,28(3):245-248.
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