植物翻译延伸因子eEF1A的研究进展
摘要
真核翻译延伸因子eEF1A(eukaryotic translation elongation factor 1A)是参与蛋白质生物合成不可或缺的一部分,eEF1A可与一些蛋白互作参与植物病毒的复制和繁殖、介导细胞死亡和早叶衰老,同时也与植物抗逆性有关,且该基因在植物中表达较广泛且稳定,因此在某些条件下可做内参基因用于基因功能分析。简要阐述植物中eEF1A的保守型、与植物抗逆性的关系、与其他蛋白互作的关系,并进行了展望。
引文
[1]陈建中,章镇,戴剑,等.黄羽扇豆翻译延伸因子2的序列分析[J].中国农业科学,2002,35(1):102-105.
[2]程君,芮耀诚.真核延长因子eEF1A功能研究进展[J].药学实践杂志,2012,30(2):89-91.
[3]RIIS B,RATTAN S I,CLARK B F,et al.Eukaryotic protein elongation factors[J].Trends in Biochemical Sciences,1990,15(11):420-424.
[4]DEVER T E,GREEN R.The Elongation,Termination,and Recycling Phases of Translation in Eukaryotes[J].Cold Spring Harbor Perspectives in Biology,2012,4(7):e013706e013711.
[5]高爽,查笑君,潘建伟.真核翻译延伸因子eEF1A功能研究进展[J].浙江师范大学学报,2013,36(4):444-449.
[6]覃迎姿,黄先益,叶兴枝,等.植物延伸因子eEF1A研究进展[J].广西农业科学,2009,40(5):472-477.
[7]METHOT S P,LITZLER L C,TRAJTENBERG F,et al.Consecutive interactions with HSP90 and eEF1A underlie a functional maturation and storage pathway of AID in the cytoplasm[J].Journal of Experimental Medicine,2015,212(4):581-596.
[8]章松柏.南方水稻黑条矮缩病的监测及其病原病毒Pns6与寄主水稻的互作[D].福州:福建农林大学,2013:31-34.
[9]BROWNING K S,HUMPHREYS J,HOBBS W,et al.Determination of the amounts of the protein synthesis initiation and elongation factors in wheat germ[J].Journal of Biological Chemistry,1990,265(29):17967-17972.
[10]张力维,李勇鹏,姚瑶,等.香樟延伸因子EF1a基因片段的克隆及表达分析[J].中南林业科技大学学报,2015(5):122-128.
[11]张佳佳,张力维,李勇鹏,等.不同非生物胁迫下香樟实时定量PCR内参基因的选择[J].生物技术通报,2016,32(10):205-211.
[12]郑丽洁,林军,黄先忠.小拟南芥实时荧光定量PCR(qRT-PCR)内参基因的筛选[J].基因组学与应用生物学,2017(2):375-384.
[13]姚正颖,张丹,李春霞,等.能源植物续随子延伸因子EF1A基因cDNA序列的克隆及分析[J].中国野生植物资源,2016,35(1):6-11.
[14]蒋素华,王默霏,宋彩霞,等.萼脊兰EF1α基因片段的克隆及序列分析[J].贵州农业科学,2016,44(5):5-8.
[15]CARNEIRO N P,HUGHES P A,LARKINS B A.The eEFlA gene family is differentially expressed in maize endosperm[J].Plant Molecular Biology,1999,41(6):801-813.
[16]SUHANDONO S,APRIYANTO A,IHSANI N.Isolationand Characterization of Three Cassava Elongation Factor 1 Alpha(MeEF1A)Promoters[J].Plos One,2014,9(1):e84692.
[17]XU WL,WANG X L,WANG H,et a1.Molecular characterization and expression analysis of nine cotton GhEFlA genes encoding translation Elongation factor l A[J].Gene,2007,389(1):27-35.
[18]LIYUAN L,XIAOJIAO H,YICUN C,et al.Identification of appropriate reference genes for normalizing transcript expression by quantitative real-time PCR in Litsea cubeba[J].Molecular Genetics&Genomics,2013,288(12):727-737.
[19]POKALSKY A R,HIAT W R,RIDGE N,et al.Structure and expression of elongation factor 1atomato[J].Nucleic Acids Research,1989,17(12):461-467.
[20]赵丽娟,袁彬青,王秀珍,等.橡胶延伸因子REF基因的克隆、转化及功能分析[J].西北农业学报,2015,24(1):144-150.
[21]张妮.香蕉MaEF1A功能的初步研究[D].海口:海南大学,2014.
[22]SUHANDONO S,APRIYANTO A,IHSANI N.Isolation and characterization of three cassava elongation factor 1alpha(MeEF1A)promoters[J].PLoSOne.2014,9(1):e84692-e84699.
[23]李子银,陈受宜.环境因子胁迫下水稻翻译延伸因子1A基因的诱导表达[J].植物学报,1999,41(8):800-806.
[24]WANG S,LEI C,WANG J,et al.SPL33,encoding an eEF1A-like protein,negatively regulates cell death and defense responses in rice[J].J.Exp.Bot.,2017,17(1):419-426.
[25]WAN H,ZHAO Z,QIAN C,et al.Selection of appropriate reference genes for gene expression studies by quan-titative real-time polymerase chain reaction in cucumber[J].Anal.Biochem.,2010,399:257-261.
[26]史兴青.甜瓜生长发育和胁迫条件下实时荧光定量PCR内参基因的筛选[D].郑州:河南农业大学,2016.
[27]彭亚兰,王友绍.红树植物桐花树EF1A基因的克隆与表达分析[J].生态科学,2014,33(4):704-712.
[28]尹佳佳.不同条件下柠条锦鸡儿荧光定量PCR内参基因的筛选[D].呼和浩特:内蒙古农业大学,2013.
[29]曾璟,王平,孙吉康,等.旱柳在Pb、Cd胁迫下实时荧光定量PCR内参基因的筛选[J].中南林业科技大学学报,2017,37(8):86-92.
[30]WANG K,NIU Y,WANG Q,et al.Cloning and evaluation of reference genes for quantitative real-time PCR analysis in Amorphophallus[J].Peerj,2017,5(4).
[31]栾鹤翔,智海剑.大豆延伸因子家族在抗大豆花叶病毒中的应用[P].中国:CN 105296531A,2016.
[32]VAYDA M E,SHEWMAKER C K,MORELLI J K.Translational arrest in hypoxic potato tubers is correlated with the aberrant association of elongation factor EF-1awith polysomes[J].Plant Mol.Bio1.,1995,28:751-757.
[33]孙伟,李燕,赵彦修,等.盐地碱蓬延伸因子(SsEF-α)的克隆与表达分析[J].西北植物学报,2004,24(9):1657-1661.
[34]SOULARD J,BOIVIN N,MORSE D,et al.eEF1AIs an S-RNase Binding Factor in Self-Incompatible Solanum chacoense[J].PLOS ONE,2014,9(2):1-7.
[35]郭艳荣,常晓月,崔晓君,等.eEF1A1基因克隆、原核分泌表达及融合蛋白纯化[J].生物技术通报,2011(6):127-133.
[36]廖文林,栾鹤翔,高乐,等.大豆翻译延伸因子GmEF1A干扰载体的构建及大豆遗传转化[J].大豆科学,2016,35(6):902-910.
[37]代广中,袁兆栋,苟洪兰,等.百脉根离子通道蛋白Pollux与翻译延伸因子EF1A的相互作用[J].华中农业大学学报,2012,31(1):34-38.
[38]ZHANG N,MCHALE L K,FINER J J.A Leader Intron of a Soybean Elongation Factor 1A(eEF1A)Gene Interacts with Proximal Promoter Elements to Regulate Gene Expression in Synthetic Promoters[J].Plos One,2016,11(11):e0166074.
[39]KANG J,LI J,GAO S,et al.Overexpression of the leucine-rich receptor-like kinase gene LRK2increases drought tolerance and tiller number in rice[J].Plant Biotechnology Journal,2017,15(9):1175-1183.
[40]ARJUN N.SASIKUMAR,WINDER B.et al.The many roles of the eukaryotic elongation factor 1complex[J].Wiley Interdisciplinary Reviews Rna,2012,3(4):543-555.
[41]周峰,刘燕,马永贵,等.真核翻译延伸因子1A蛋白家族功能位点的进化踪迹分析[J].中国生物化学与分子生物学报,2013,29(8):773-782.
[42]胡瑞波,范成明,傅永福.植物实时荧光定量PCR内参基因的选择[J].中国农业科技导报,2009,11(6):30-36.
[43]ACEVEDO R M,AVICO E H,RUIZ O A,et al.Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought[J].Biologia Plantarum,2017,9(6):98164-98172.
[44]HABBEN J E,MORO G L,HUNTER B G,et a1.Elongation factor 1alpha concentration is highly correlated with the lysine content of maize endosperm[J].Proc Natl Acad Sci USA,1995,92(19):8640-8644.
[45]SARAIVA,KáTIA DANIELLA DA CRUZ,OL-IVEIRA A E R,et al.Phylogenetic analysis and differential expression ofEF1αgenes in soybean during development,stress and phytohormone treatments[J].Molecular Genetics and Genomics,2016,291(40):1505-1522.
[2]程君,芮耀诚.真核延长因子eEF1A功能研究进展[J].药学实践杂志,2012,30(2):89-91.
[3]RIIS B,RATTAN S I,CLARK B F,et al.Eukaryotic protein elongation factors[J].Trends in Biochemical Sciences,1990,15(11):420-424.
[4]DEVER T E,GREEN R.The Elongation,Termination,and Recycling Phases of Translation in Eukaryotes[J].Cold Spring Harbor Perspectives in Biology,2012,4(7):e013706e013711.
[5]高爽,查笑君,潘建伟.真核翻译延伸因子eEF1A功能研究进展[J].浙江师范大学学报,2013,36(4):444-449.
[6]覃迎姿,黄先益,叶兴枝,等.植物延伸因子eEF1A研究进展[J].广西农业科学,2009,40(5):472-477.
[7]METHOT S P,LITZLER L C,TRAJTENBERG F,et al.Consecutive interactions with HSP90 and eEF1A underlie a functional maturation and storage pathway of AID in the cytoplasm[J].Journal of Experimental Medicine,2015,212(4):581-596.
[8]章松柏.南方水稻黑条矮缩病的监测及其病原病毒Pns6与寄主水稻的互作[D].福州:福建农林大学,2013:31-34.
[9]BROWNING K S,HUMPHREYS J,HOBBS W,et al.Determination of the amounts of the protein synthesis initiation and elongation factors in wheat germ[J].Journal of Biological Chemistry,1990,265(29):17967-17972.
[10]张力维,李勇鹏,姚瑶,等.香樟延伸因子EF1a基因片段的克隆及表达分析[J].中南林业科技大学学报,2015(5):122-128.
[11]张佳佳,张力维,李勇鹏,等.不同非生物胁迫下香樟实时定量PCR内参基因的选择[J].生物技术通报,2016,32(10):205-211.
[12]郑丽洁,林军,黄先忠.小拟南芥实时荧光定量PCR(qRT-PCR)内参基因的筛选[J].基因组学与应用生物学,2017(2):375-384.
[13]姚正颖,张丹,李春霞,等.能源植物续随子延伸因子EF1A基因cDNA序列的克隆及分析[J].中国野生植物资源,2016,35(1):6-11.
[14]蒋素华,王默霏,宋彩霞,等.萼脊兰EF1α基因片段的克隆及序列分析[J].贵州农业科学,2016,44(5):5-8.
[15]CARNEIRO N P,HUGHES P A,LARKINS B A.The eEFlA gene family is differentially expressed in maize endosperm[J].Plant Molecular Biology,1999,41(6):801-813.
[16]SUHANDONO S,APRIYANTO A,IHSANI N.Isolationand Characterization of Three Cassava Elongation Factor 1 Alpha(MeEF1A)Promoters[J].Plos One,2014,9(1):e84692.
[17]XU WL,WANG X L,WANG H,et a1.Molecular characterization and expression analysis of nine cotton GhEFlA genes encoding translation Elongation factor l A[J].Gene,2007,389(1):27-35.
[18]LIYUAN L,XIAOJIAO H,YICUN C,et al.Identification of appropriate reference genes for normalizing transcript expression by quantitative real-time PCR in Litsea cubeba[J].Molecular Genetics&Genomics,2013,288(12):727-737.
[19]POKALSKY A R,HIAT W R,RIDGE N,et al.Structure and expression of elongation factor 1atomato[J].Nucleic Acids Research,1989,17(12):461-467.
[20]赵丽娟,袁彬青,王秀珍,等.橡胶延伸因子REF基因的克隆、转化及功能分析[J].西北农业学报,2015,24(1):144-150.
[21]张妮.香蕉MaEF1A功能的初步研究[D].海口:海南大学,2014.
[22]SUHANDONO S,APRIYANTO A,IHSANI N.Isolation and characterization of three cassava elongation factor 1alpha(MeEF1A)promoters[J].PLoSOne.2014,9(1):e84692-e84699.
[23]李子银,陈受宜.环境因子胁迫下水稻翻译延伸因子1A基因的诱导表达[J].植物学报,1999,41(8):800-806.
[24]WANG S,LEI C,WANG J,et al.SPL33,encoding an eEF1A-like protein,negatively regulates cell death and defense responses in rice[J].J.Exp.Bot.,2017,17(1):419-426.
[25]WAN H,ZHAO Z,QIAN C,et al.Selection of appropriate reference genes for gene expression studies by quan-titative real-time polymerase chain reaction in cucumber[J].Anal.Biochem.,2010,399:257-261.
[26]史兴青.甜瓜生长发育和胁迫条件下实时荧光定量PCR内参基因的筛选[D].郑州:河南农业大学,2016.
[27]彭亚兰,王友绍.红树植物桐花树EF1A基因的克隆与表达分析[J].生态科学,2014,33(4):704-712.
[28]尹佳佳.不同条件下柠条锦鸡儿荧光定量PCR内参基因的筛选[D].呼和浩特:内蒙古农业大学,2013.
[29]曾璟,王平,孙吉康,等.旱柳在Pb、Cd胁迫下实时荧光定量PCR内参基因的筛选[J].中南林业科技大学学报,2017,37(8):86-92.
[30]WANG K,NIU Y,WANG Q,et al.Cloning and evaluation of reference genes for quantitative real-time PCR analysis in Amorphophallus[J].Peerj,2017,5(4).
[31]栾鹤翔,智海剑.大豆延伸因子家族在抗大豆花叶病毒中的应用[P].中国:CN 105296531A,2016.
[32]VAYDA M E,SHEWMAKER C K,MORELLI J K.Translational arrest in hypoxic potato tubers is correlated with the aberrant association of elongation factor EF-1awith polysomes[J].Plant Mol.Bio1.,1995,28:751-757.
[33]孙伟,李燕,赵彦修,等.盐地碱蓬延伸因子(SsEF-α)的克隆与表达分析[J].西北植物学报,2004,24(9):1657-1661.
[34]SOULARD J,BOIVIN N,MORSE D,et al.eEF1AIs an S-RNase Binding Factor in Self-Incompatible Solanum chacoense[J].PLOS ONE,2014,9(2):1-7.
[35]郭艳荣,常晓月,崔晓君,等.eEF1A1基因克隆、原核分泌表达及融合蛋白纯化[J].生物技术通报,2011(6):127-133.
[36]廖文林,栾鹤翔,高乐,等.大豆翻译延伸因子GmEF1A干扰载体的构建及大豆遗传转化[J].大豆科学,2016,35(6):902-910.
[37]代广中,袁兆栋,苟洪兰,等.百脉根离子通道蛋白Pollux与翻译延伸因子EF1A的相互作用[J].华中农业大学学报,2012,31(1):34-38.
[38]ZHANG N,MCHALE L K,FINER J J.A Leader Intron of a Soybean Elongation Factor 1A(eEF1A)Gene Interacts with Proximal Promoter Elements to Regulate Gene Expression in Synthetic Promoters[J].Plos One,2016,11(11):e0166074.
[39]KANG J,LI J,GAO S,et al.Overexpression of the leucine-rich receptor-like kinase gene LRK2increases drought tolerance and tiller number in rice[J].Plant Biotechnology Journal,2017,15(9):1175-1183.
[40]ARJUN N.SASIKUMAR,WINDER B.et al.The many roles of the eukaryotic elongation factor 1complex[J].Wiley Interdisciplinary Reviews Rna,2012,3(4):543-555.
[41]周峰,刘燕,马永贵,等.真核翻译延伸因子1A蛋白家族功能位点的进化踪迹分析[J].中国生物化学与分子生物学报,2013,29(8):773-782.
[42]胡瑞波,范成明,傅永福.植物实时荧光定量PCR内参基因的选择[J].中国农业科技导报,2009,11(6):30-36.
[43]ACEVEDO R M,AVICO E H,RUIZ O A,et al.Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought[J].Biologia Plantarum,2017,9(6):98164-98172.
[44]HABBEN J E,MORO G L,HUNTER B G,et a1.Elongation factor 1alpha concentration is highly correlated with the lysine content of maize endosperm[J].Proc Natl Acad Sci USA,1995,92(19):8640-8644.
[45]SARAIVA,KáTIA DANIELLA DA CRUZ,OL-IVEIRA A E R,et al.Phylogenetic analysis and differential expression ofEF1αgenes in soybean during development,stress and phytohormone treatments[J].Molecular Genetics and Genomics,2016,291(40):1505-1522.
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