我国海洋微生物菌种资源保藏与共享服务现状
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摘要
海洋微生物多样性丰富。我国近年来在海洋微生物多样性调查、资源获取与开发利用等多方面取得重要进展。为促进资源的可持续高效利用,建立了专业的海洋微生物菌种保藏中心,目前库藏海洋微生物菌种2. 2万株。本文介绍了目前中国海洋微生物菌种保藏管理中心库藏资源概况与共享利用情况,以共享记录为基础,从已共享菌株的多样性、库藏资源共享利用以及共享用户等方面开展了统计分析。统计结果显示,共享菌株中超过80%为细菌,其次依次为丝状真菌、酵母、古菌及噬菌体。在菌株水平,已共享细菌覆盖了库藏细菌的28%;在种的水平,已共享细菌覆盖了库藏细菌的47%,包括变形菌纲3 178株,芽胞杆菌纲951株,放线菌纲817株。目前,库藏资源总共享比例约为30%,覆盖了库藏57%的属和49%的种。共享用户目前达266家,其中国内用户244家,国外用户22家。为大洋课题、国家973课题、863课题、自然基金、行业公益性项目、科技支撑及企业项目等各种科技计划,提供了重要的菌种资源保障。据不完全统计,共支撑发表SCI论文达500余篇。中国海洋微生物菌种保藏管理中心在菌株资源的收集、整理和共享等方面,为我国的科研事业提供了大量的资源支撑,取得了较好的社会效益。
Marine microbial resources are abundant in diversity. In recent years,China has made important progress in marine microbial diversity survey,resource acquisition and utilization. In order to promote the sustainable and efficient use of the resources,we have established a professional marine culture collection center,which currently maintains 22,000 strains. This paper introduces the general situation and sharing of marine microbial resources in Marine Culture Collection of China. Based on the original records and database,the diversity of the shared strains,the sharing and utilization of the repository resources and the users are analyzed statistically. Statistics showed that more than 80% of the shared strains were bacteria,followed by filamentous fungi,yeast,archaea and phages. At the strain level,the shared bacteria covered 28% of the reservoir bacteria;at the species level,they covered 47% of the reservoir bacteria. The shared bacteria include 3 178 strains of Proteobacteria,951 strains of Bacilli and 817 strains of Actinomycetes. At present,the gen-gral utilization of the total resources is about 30%,covering 57% of the total genera and 49% of the total species. There are currently 266 unites of strain users,including 244 domestic and 22 foreign users. It provides an important support of microbial resources for various scientific and technological projects in China such as Ocean Project,National 973 Project,863 Project,Natural Fund,Industry Public Welfare Project,Scientific and Technological Support and Enterprise Project. According to incomplete statistics,more than 500 SCI papers have been published under the microbial resources supported by Marine Culture Collection of China. Generally,it has provided a large number of resource support to Chinese scientific research.
Marine microbial resources are abundant in diversity. In recent years,China has made important progress in marine microbial diversity survey,resource acquisition and utilization. In order to promote the sustainable and efficient use of the resources,we have established a professional marine culture collection center,which currently maintains 22,000 strains. This paper introduces the general situation and sharing of marine microbial resources in Marine Culture Collection of China. Based on the original records and database,the diversity of the shared strains,the sharing and utilization of the repository resources and the users are analyzed statistically. Statistics showed that more than 80% of the shared strains were bacteria,followed by filamentous fungi,yeast,archaea and phages. At the strain level,the shared bacteria covered 28% of the reservoir bacteria;at the species level,they covered 47% of the reservoir bacteria. The shared bacteria include 3 178 strains of Proteobacteria,951 strains of Bacilli and 817 strains of Actinomycetes. At present,the gen-gral utilization of the total resources is about 30%,covering 57% of the total genera and 49% of the total species. There are currently 266 unites of strain users,including 244 domestic and 22 foreign users. It provides an important support of microbial resources for various scientific and technological projects in China such as Ocean Project,National 973 Project,863 Project,Natural Fund,Industry Public Welfare Project,Scientific and Technological Support and Enterprise Project. According to incomplete statistics,more than 500 SCI papers have been published under the microbial resources supported by Marine Culture Collection of China. Generally,it has provided a large number of resource support to Chinese scientific research.
引文
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[2] Delong E F. Modern microbial seascapes[J]. Nat Rev Microbiol, 2007, 5(10):755-757.
[3] Bowler C, Karl D M, Colwell R R. Microbial oceanog-raphy in a sea of opportunity[J]. Nature, 2009, 459(7244):180-184.
[4] Karl D M. Microbial oceanography:paradigms, pro-cesses and promise[J]. Nat Rev Microbiol, 2007, 5(10):759-769.
[5] Warner R. Oceans beyond boundaries:environmental assessment frameworks[J]. Int J Mar Coast Law,2012, 27:481-499.
[6] Blasiak R, Yagi N. Shaping an international agreement on marine biodiversity beyond areas of national jurisdic-tion:lessons from highseas fisheries[J]. Marine Policy,2016, 71:210-216.
[7] Ribeiro M C. South Atlantic perspectives on the future international legally binding instrument under the LOSC on conservation and sustainable use of BBNJ[J]. Int J Mar Coast Law, 2017, 32:733-764.
[8] Liu N Z, Gao Y Y. Comment on international agree-ment on marine biodiversity beyond areas of national ju-risdiction and China’s corresponding strategies[J]. Ocean Development and Management, 2018, 7:10-15.刘乃忠,高莹莹.国家管辖范围外海洋生物多样性养护与可持续利用国际协定重点问题评析与中国应对策略[J].海洋开发与管理, 2018, 7:10-15.
[9] Lucia V D. The arctic environment and the BBNJ nego-tiations. Special rules for special circumstances?[J].Marine Policy, 2017, 86:234-240.
[10] Lin Y F."The project of pilot in sorting, integrating and sharing marine microbial culture resources"has achieved remarkable results[J]. Marine Information,2009, 3:32.林岳夫.“海洋微生物菌种资源整理整合与共享试点”成果斐然[J].海洋信息, 2009, 3:32.
[11] Jiang R B. Rules for description of microorganism re-sources[M]. Beijing:China Agricultural Science and Technology Press, 2006.姜瑞波.微生物菌种资源描述规范[M].北京:中国农业科学技术出版社, 2006.
[12] Cao Y H, Liu X. Rules for general description of natu-ral science and technology resources[M]. Beijing:China Science and Technology Press, 2006.曹一化,刘旭.自然科技资源共性描述规范[M].北京:中国科学技术出版社, 2006.
[13]“Microbial Culture Resources”project team of National Infrastructure of Natural Resources for Science and Technology. Compilation of rules for microbial culture resources description[M]. Beijing:China Agricultural Science and Technology Press, 2009.国家自然科技资源平台“微生物菌种资源”项目组.微生物菌种资源描述规范汇编[M].北京:中国农业科学技术出版社, 2009.
[14] Shao Z Z. Catalogue of China Marine Microbial Cul-tures[M]. Beijing:Chemical Industry Press. 2010.邵宗泽.中国海洋微生物菌种目录[M].北京:化学工业出版社, 2010.
[15] Wang W, Shao Z Z. The long-chain alkane metabolism network of Alcanivorax dieselolei[J]. Nat Commun,2014, 5:5 755-5 766.
[16] Ross A C, Xu Y, Lu L, et al. Biosynthetic multitask-ing facilitates thalassospiramide structural diversity in marine bacteria[J]. J Am Chem Soc, 2013, 135(3):1155-1 162.
[17] Shao Z Z, Wang W P, Zhuang L P, et al. Strain s21-t17(H)-N3, a PAHs degrading bacteria and its applica-tion:China, 201410248117. 1[P]. 2014-08-27.邵宗泽,王万鹏,庄玲萍,等.一株多环芳烃降解菌s21-t17(H)-N3及其应用:中国, 201410248117. 1[P].2014-08-27.
[18] Li H R, Li H S. A biocathode microbial fuel cell for wastewater treatment system and its method for waste-water treatment:China, 201510172618. 0[P]. 2015-04-13.李浩然,李洪珊.一种生物阴极型微生物燃料电池废水处理系统和处理废水的方法:中国, 201510172618. 0[P]. 2015-07-15.
[19] Shao Z Z, Wang L P, Shan D P, et al. A kind of mi-croecological agent as well as its preparation method and application:China, ZL201310333941. 2[P]. 2014-01-08.邵宗泽,王丽萍,单大鹏,等.一种微生态制剂及其制备方法与应用:中国, ZL201310333941[P]. 2014-01-08.
[20] Zhang J B, Shao Z Z, Yu Z N, et al. The application of fermentation supernatant from a strain of Virgibacil-lus dokdonensis:China, 201410157288[P]. 2014-07-16.张吉斌,邵宗泽,喻子牛,等.一种独岛枝芽胞杆菌发酵上清液的应用:中国, 201410157288[P]. 2014-07-16.
[21] Zhou M X, Chen X Y, Ruan L W. Preparation and ap-plication of a kind of alkaline pullulanase:China,ZL201310250392. 2[P]. 2014-02-12.周梅先,陈逍遥,阮灵伟.一种碱性普鲁兰酶的制备方法及应用:中国, ZL201310250392. 2[P]. 2014-02-12.
[22] Lin W H, Zhou D M, Zhao Y, et al. Preparation of three new isoprene cyclohexanol componds and their anti-influenza applications:China, 201510057980. 3[P].2016-10-05.林文翰,周德敏,赵阳,等.三个新异戊二烯环己醇类化合物的制备方法及其抗流感用途:中国,201510057980. 3[P]. 2016-10-05.
[23] Yang X W, Niu S W, Xia J M, et al. Botryotinia fuck-eliana MCCC 3A00494 and its fermentation compounds in the application of preparing antineoplastic drugs:Chi-na, 201711258939. 8[P]. 2018-06-15.杨献文,牛四文,夏金梅,等.富克尔核盘菌3A00494及其发酵化合物在制备抗肿瘤药物中的应用:中国,201711258939. 8[P]. 2018-06-15.
[24] Lu M, Lin W H, et al. A preparation method of natural products from marine fungi and the application of natural products in lipid-lowering:China, 201510178049. 0[P]. 2015-05-06.路萌,林文翰,等.一种源于海洋真菌的天然产物的制备方法及其降脂用途:中国, 201510178049. 0[P].2015-05-06.
[25] Niu S, Fan Z, Tang X, et al. Cyclopiane-type diter-penes from the deep-sea-derived fungus Penicillium commune MCCC 3A00940[J]. Tetrahedron Lett,2018, 59(4):375-378.
[26] Niu S, Liu Q, Xia J M, et al. Polyketides from the deep-sea-derived fungus Graphostroma sp. MCCC3A00421 showed potent antifood allergic activities[J].J Agric Food Chem, 2018, 66(6):1 369-1 376.
[27] Niu S, Wang N, Xie C L, et al. Roquefortine J, a nov-el roquefortine alkaloid, from the deep-sea-derived fun-gus Penicillium granulatum MCCC 3A00475[J]. J An-tibiot(Tokyo), 2018, 71(7):658-661.
[28] Niu S, Xie C L, Xia J M, et al. New anti-inflammatory guaianes from the Atlantic hydrotherm-derived fungus Graphostroma sp. MCCC 3A00421[J]. Sci Rep,2018, 8(1):530.
[29] Xie C L, Niu S, Xia J M, et al. Saccharopolytide A, a new cyclic tetrapeptide with rare 4-hydroxy-proline moi-eties from the deep-sea derived actinomycete Saccha-ropolyspora cebuensis MCCC 1A09850[J]. Nat Prod Res, 2018, 32(14):1 627-1 631.
[30] Yang X W, Xie C L, Xia J M, et al. The application of fermentation compounds from Aspergillus terrestris H768 in the preparation of antiallergic drugs:China,201710986477. 5[P]. 2018-04-10.杨献文,谢春兰,夏金梅,等.土曲霉H768的发酵化合物在制备抗过敏药物中的应用:中国,201710986477. 5[P]. 2018-04-10.
[31] Yang X W, Niu S W, Xia J M, et al. Extraction and purification method of Penicillium T3-1 and its fermen-tation compounds as well as their application in antialler-gy:China, 201710541336. 2[P]. 2017-09-19.杨献文,牛四文,夏金梅,等.团青霉T3-1及其发酵化合物、提取纯化方法和在抗过敏中的应用:中国,201710541336. 2[P]. 2017-09-19.
[32] Yang X W, Fan Z W, Tang X X, et al. Preparation of three phenolic derivatives and their application in the preparation of anti-HIV drugs:China, 201611254513. 0[P]. 2017-05-17.杨献文,范佐旺,汤熙翔,等.三种苯酚类衍生物的制备方法及其在制备抗HIV药物中的应用:中国,201611254513. 0[P]. 2017-05-17.
[33] Yang X W, Niu S W, Xia J M, et al. The application of deep-sea strain MCCC 3A00421 and its fermentation compounds:China, 201711366810. 9[P]. 2018-07-10.杨献文,牛四文,夏金梅,等.深海真菌3A00421及其发酵化合物的应用:中国, 201711366810. 9[P]. 2018-07-10.
[34] Lu L, Wang J, Xu Y, et al. A high-resolution LC-MS-based secondary metabolite fingerprint database of ma-rine bacteria[J]. Sci Rep, 2014, 4:6 537-6 514.
[2] Delong E F. Modern microbial seascapes[J]. Nat Rev Microbiol, 2007, 5(10):755-757.
[3] Bowler C, Karl D M, Colwell R R. Microbial oceanog-raphy in a sea of opportunity[J]. Nature, 2009, 459(7244):180-184.
[4] Karl D M. Microbial oceanography:paradigms, pro-cesses and promise[J]. Nat Rev Microbiol, 2007, 5(10):759-769.
[5] Warner R. Oceans beyond boundaries:environmental assessment frameworks[J]. Int J Mar Coast Law,2012, 27:481-499.
[6] Blasiak R, Yagi N. Shaping an international agreement on marine biodiversity beyond areas of national jurisdic-tion:lessons from highseas fisheries[J]. Marine Policy,2016, 71:210-216.
[7] Ribeiro M C. South Atlantic perspectives on the future international legally binding instrument under the LOSC on conservation and sustainable use of BBNJ[J]. Int J Mar Coast Law, 2017, 32:733-764.
[8] Liu N Z, Gao Y Y. Comment on international agree-ment on marine biodiversity beyond areas of national ju-risdiction and China’s corresponding strategies[J]. Ocean Development and Management, 2018, 7:10-15.刘乃忠,高莹莹.国家管辖范围外海洋生物多样性养护与可持续利用国际协定重点问题评析与中国应对策略[J].海洋开发与管理, 2018, 7:10-15.
[9] Lucia V D. The arctic environment and the BBNJ nego-tiations. Special rules for special circumstances?[J].Marine Policy, 2017, 86:234-240.
[10] Lin Y F."The project of pilot in sorting, integrating and sharing marine microbial culture resources"has achieved remarkable results[J]. Marine Information,2009, 3:32.林岳夫.“海洋微生物菌种资源整理整合与共享试点”成果斐然[J].海洋信息, 2009, 3:32.
[11] Jiang R B. Rules for description of microorganism re-sources[M]. Beijing:China Agricultural Science and Technology Press, 2006.姜瑞波.微生物菌种资源描述规范[M].北京:中国农业科学技术出版社, 2006.
[12] Cao Y H, Liu X. Rules for general description of natu-ral science and technology resources[M]. Beijing:China Science and Technology Press, 2006.曹一化,刘旭.自然科技资源共性描述规范[M].北京:中国科学技术出版社, 2006.
[13]“Microbial Culture Resources”project team of National Infrastructure of Natural Resources for Science and Technology. Compilation of rules for microbial culture resources description[M]. Beijing:China Agricultural Science and Technology Press, 2009.国家自然科技资源平台“微生物菌种资源”项目组.微生物菌种资源描述规范汇编[M].北京:中国农业科学技术出版社, 2009.
[14] Shao Z Z. Catalogue of China Marine Microbial Cul-tures[M]. Beijing:Chemical Industry Press. 2010.邵宗泽.中国海洋微生物菌种目录[M].北京:化学工业出版社, 2010.
[15] Wang W, Shao Z Z. The long-chain alkane metabolism network of Alcanivorax dieselolei[J]. Nat Commun,2014, 5:5 755-5 766.
[16] Ross A C, Xu Y, Lu L, et al. Biosynthetic multitask-ing facilitates thalassospiramide structural diversity in marine bacteria[J]. J Am Chem Soc, 2013, 135(3):1155-1 162.
[17] Shao Z Z, Wang W P, Zhuang L P, et al. Strain s21-t17(H)-N3, a PAHs degrading bacteria and its applica-tion:China, 201410248117. 1[P]. 2014-08-27.邵宗泽,王万鹏,庄玲萍,等.一株多环芳烃降解菌s21-t17(H)-N3及其应用:中国, 201410248117. 1[P].2014-08-27.
[18] Li H R, Li H S. A biocathode microbial fuel cell for wastewater treatment system and its method for waste-water treatment:China, 201510172618. 0[P]. 2015-04-13.李浩然,李洪珊.一种生物阴极型微生物燃料电池废水处理系统和处理废水的方法:中国, 201510172618. 0[P]. 2015-07-15.
[19] Shao Z Z, Wang L P, Shan D P, et al. A kind of mi-croecological agent as well as its preparation method and application:China, ZL201310333941. 2[P]. 2014-01-08.邵宗泽,王丽萍,单大鹏,等.一种微生态制剂及其制备方法与应用:中国, ZL201310333941[P]. 2014-01-08.
[20] Zhang J B, Shao Z Z, Yu Z N, et al. The application of fermentation supernatant from a strain of Virgibacil-lus dokdonensis:China, 201410157288[P]. 2014-07-16.张吉斌,邵宗泽,喻子牛,等.一种独岛枝芽胞杆菌发酵上清液的应用:中国, 201410157288[P]. 2014-07-16.
[21] Zhou M X, Chen X Y, Ruan L W. Preparation and ap-plication of a kind of alkaline pullulanase:China,ZL201310250392. 2[P]. 2014-02-12.周梅先,陈逍遥,阮灵伟.一种碱性普鲁兰酶的制备方法及应用:中国, ZL201310250392. 2[P]. 2014-02-12.
[22] Lin W H, Zhou D M, Zhao Y, et al. Preparation of three new isoprene cyclohexanol componds and their anti-influenza applications:China, 201510057980. 3[P].2016-10-05.林文翰,周德敏,赵阳,等.三个新异戊二烯环己醇类化合物的制备方法及其抗流感用途:中国,201510057980. 3[P]. 2016-10-05.
[23] Yang X W, Niu S W, Xia J M, et al. Botryotinia fuck-eliana MCCC 3A00494 and its fermentation compounds in the application of preparing antineoplastic drugs:Chi-na, 201711258939. 8[P]. 2018-06-15.杨献文,牛四文,夏金梅,等.富克尔核盘菌3A00494及其发酵化合物在制备抗肿瘤药物中的应用:中国,201711258939. 8[P]. 2018-06-15.
[24] Lu M, Lin W H, et al. A preparation method of natural products from marine fungi and the application of natural products in lipid-lowering:China, 201510178049. 0[P]. 2015-05-06.路萌,林文翰,等.一种源于海洋真菌的天然产物的制备方法及其降脂用途:中国, 201510178049. 0[P].2015-05-06.
[25] Niu S, Fan Z, Tang X, et al. Cyclopiane-type diter-penes from the deep-sea-derived fungus Penicillium commune MCCC 3A00940[J]. Tetrahedron Lett,2018, 59(4):375-378.
[26] Niu S, Liu Q, Xia J M, et al. Polyketides from the deep-sea-derived fungus Graphostroma sp. MCCC3A00421 showed potent antifood allergic activities[J].J Agric Food Chem, 2018, 66(6):1 369-1 376.
[27] Niu S, Wang N, Xie C L, et al. Roquefortine J, a nov-el roquefortine alkaloid, from the deep-sea-derived fun-gus Penicillium granulatum MCCC 3A00475[J]. J An-tibiot(Tokyo), 2018, 71(7):658-661.
[28] Niu S, Xie C L, Xia J M, et al. New anti-inflammatory guaianes from the Atlantic hydrotherm-derived fungus Graphostroma sp. MCCC 3A00421[J]. Sci Rep,2018, 8(1):530.
[29] Xie C L, Niu S, Xia J M, et al. Saccharopolytide A, a new cyclic tetrapeptide with rare 4-hydroxy-proline moi-eties from the deep-sea derived actinomycete Saccha-ropolyspora cebuensis MCCC 1A09850[J]. Nat Prod Res, 2018, 32(14):1 627-1 631.
[30] Yang X W, Xie C L, Xia J M, et al. The application of fermentation compounds from Aspergillus terrestris H768 in the preparation of antiallergic drugs:China,201710986477. 5[P]. 2018-04-10.杨献文,谢春兰,夏金梅,等.土曲霉H768的发酵化合物在制备抗过敏药物中的应用:中国,201710986477. 5[P]. 2018-04-10.
[31] Yang X W, Niu S W, Xia J M, et al. Extraction and purification method of Penicillium T3-1 and its fermen-tation compounds as well as their application in antialler-gy:China, 201710541336. 2[P]. 2017-09-19.杨献文,牛四文,夏金梅,等.团青霉T3-1及其发酵化合物、提取纯化方法和在抗过敏中的应用:中国,201710541336. 2[P]. 2017-09-19.
[32] Yang X W, Fan Z W, Tang X X, et al. Preparation of three phenolic derivatives and their application in the preparation of anti-HIV drugs:China, 201611254513. 0[P]. 2017-05-17.杨献文,范佐旺,汤熙翔,等.三种苯酚类衍生物的制备方法及其在制备抗HIV药物中的应用:中国,201611254513. 0[P]. 2017-05-17.
[33] Yang X W, Niu S W, Xia J M, et al. The application of deep-sea strain MCCC 3A00421 and its fermentation compounds:China, 201711366810. 9[P]. 2018-07-10.杨献文,牛四文,夏金梅,等.深海真菌3A00421及其发酵化合物的应用:中国, 201711366810. 9[P]. 2018-07-10.
[34] Lu L, Wang J, Xu Y, et al. A high-resolution LC-MS-based secondary metabolite fingerprint database of ma-rine bacteria[J]. Sci Rep, 2014, 4:6 537-6 514.
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