中药对猪场常见病原菌的药敏特性及联合抑菌活性评价

详细信息    查看全文 | 推荐本文 |

英文篇名：Susceptibility of Common Pathogenic Bacteria in Pig Farm to Traditional Chinese Medicines and Their Combined Bacteriostatic Activity 作者：姜源明 ; 俸祥仁 ; 兰宗宝 ; 陈德元 ; 赵武 ; 刘伟 ; 银慧慧 ; 孙建华 英文作者：JIANG Yuan-ming;FENG Xiang-ren;LAN Zong-bao;CHEN De-yuan;ZHAO Wu;LIU Wei;YIN Hui-hui;SUN Jian-hua;Guangxi Veterinary Research Institute;Qiangwei Agriculture and Animal Husbandry Technology Limited Company in Nanning of Guangxi;Baipeng Stud Farm of Guangxi;Institute of Agricultural Science and Technology Information,Guangxi Academy of Agricultural Sciences; 关键词：中药 ; 病原菌 ; 药敏试验 ; 最小抑菌浓度 ; 联合抑菌活性 英文关键词：Traditional Chinese medicine;;Pathogenic bacteria;;Drug susceptibility test;;Minimal inhibitory concentration;;Combined bacteriostatic activity 中文刊名：江西农业学报 英文刊名：Acta Agriculturae Jiangxi 机构：广西兽医研究所;广西南宁强微农牧科技有限公司;广西百朋种畜场;广西农业科学院农业科技信息研究所; 出版日期：2019-02-15 出版单位：江西农业学报 年：2019 期：02 基金：广西南宁市科学研究与技术开发计划项目(20152309);; 广西水产畜牧科技推广应用项目(桂渔牧科201528001);; 广西南宁市西乡塘区科学研究与技术开发计划项目(20810203) 语种：中文; 页：84-89 页数：6 CN：36-1124/S ISSN：1001-8581 分类号：S852.61

摘要

以常见的50味中药提取液对大肠杆菌、巴氏杆菌、链球菌、沙门氏菌、金黄色葡萄球菌和放线杆菌等6种猪场常见病原菌进行药敏试验,并在测定单味中药对上述病原菌最小抑菌浓度(MIC)的基础上,采用微量棋盘稀释法进行中药两两联用抑菌活性试验,通过计算抑菌浓度指数(FICI)判断药物互作效应。药敏试验结果表明,乌梅、大黄、黄芩、金银花、连翘、野菊花和五倍子对6种猪场常见病原菌均表现为高度敏感。药物MIC测定结果显示,以乌梅、大黄、桂枝、金银花、连翘、野菊花和五倍子等7味中药对6种猪场常见病原菌的MIC较低,故选取这7味中药进行两两联合用药试验。在中药两两联用抑菌活性试验中,乌梅+桂枝、大黄+桂枝、桂枝+连翘、桂枝+野菊花、桂枝+五倍子呈拮抗效应;乌梅+五倍子、大黄+连翘、大黄+野菊花、大黄+五倍子、金银花+连翘、金银花+野菊花、连翘+野菊花呈协同效应;乌梅+大黄、乌梅+金银花、乌梅+连翘、乌梅+野菊花、大黄+金银花、桂枝+金银花、金银花+五倍子、连翘+五倍子、野菊花+五倍子呈相加效应。
        The single-factor antibacterial effects and two-by-two combined bacteriostatic activities of 50 kinds of traditional Chinese medicine extraction solutions to 6 common pathogenic bacteria(Escherichia coli,Pasteurella,Streptococcus,Salmonella,Staphylococcus aureus and Actinobacillus) in pig farm were studied through drug susceptibility test and by using microdilution checkerboard technique. The results of drug susceptibility test showed that the above 6 pathogenic bacteria were all highly susceptible to dark plum,Chinese rhubarb,baikal skullcap root,honeysuckle,forsythia,wild chrysanthemum and gallnut. The results of test for Minimal Inhibitory Concentration(MIC) of traditional Chinese medicines indicated that dark plum,Chinese rhubarb,cassia twig,honeysuckle,forsythia,wild chrysanthemum and gallnut all had a lower MIC against above 6 pathogenic bacteria in pig farm,so these 7 kinds of traditional Chinese medicines were selected to conduct the two-by-two combined bacteriostatic test.In the two-by-two combined bacteriostatic activity tests,by calculating the Fractional Inhibitory Concentration Index(FICI),dark plum+ cassia twig,Chinese rhubarb+ cassia twig,cassia twig+ forsythia,cassia twig+ wild chrysanthemum,and cassia twig+ gallnut had an antagonistic effect; dark plum + gallnut,Chinese rhubarb + forsythia,Chinese rhubarb + wild chrysanthemum,Chinese rhubarb+ gallnut,honeysuckle+ forsythia,honeysuckle+ wild chrysanthemum,and forsythia+ wild chrysanthemum had a synergistic effect; dark plum+ Chinese rhubarb,dark plum + honeysuckle,dark plum + forsythia,dark plum + wild chrysanthemum,Chinese rhubarb+ honeysuckle,cassia twig+ honeysuckle,honeysuckle+ gallnut,forsythia+ gallnut,and wild chrysanthemum+ gallnut had an additive effect.

引文

[1]李冰.中药在畜牧业应用中存在的问题及其对策[J].畜牧与饲料科学,2014,35(9):92-93.
    [2]叶茂.中草药在畜产品安全生产中的作用与中兽药研发方向[J].畜禽业,2012(4):39-40.
    [3]陈培军.中兽医药与畜牧业的健康养殖[J].中国动物保健,2017,19(3):73-74.
    [4]姜源明,刘伟,赵武,等.中兽药产业可持续发展的问题与对策[J].中国畜禽种业,2017,13(6):13-15.
    [5]刘增援,吴永继,黄海澄,等.透骨草水提物与抗菌药联合用药对耐药大肠杆菌的抑菌效果[J].南方农业学报,2015,46(8):1522-1526.
    [6]柴贝贝,姜维佳,王莉贞,等.原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果[J].南方农业学报,2018,49(3):580-585.
    [7]宋剑武,陈秋莹,夏娟,等.救必应水提物与抗菌药联用对产ESBLs细菌的抑制效果[J].南方农业学报,2014,45(8):1470-1473.
    [8]吴永继,刘增援,孙燕杰,等.爬山虎水提液与抗菌药联用对含fosA3基因大肠杆菌的抑菌效果[J].南方农业学报,2015,46(4):707-711.
    [9]赵明军,张洪玉,周状.中草药对水产动物疾病防治的药效学研究综述[J].安徽农业科学,2009,37(36):8010-8015,8036.
    [10]李艳华,闫清波,于康震,等.复方中药对禽流感病毒的防治研究[J].中国农业科学,2008,41(5):1511-1518.