亚麻COBRA基因家族的鉴定与生物信息学分析

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英文篇名：Identification and Bioinformatics Analysis of the COBRA Gene Family in Flax 作者：齐燕妮 ; 李闻娟 ; 王利民 ; 赵玮 ; 党照 ; 谢亚萍 ; 张建平 英文作者：QI Yanni;LI Wenjuan;WANG Limin;ZHAO Wei;DANG Zhao;XIE Yaping;ZHANG Jianping;Institute of Crops,Gansu Academy of Agricultural Sciences; 关键词：亚麻 ; 纤维 ; COBRA基因家族 ; 序列分析 ; 系统进化分析 英文关键词：Flax;;Fiber;;COBRA gene family;;Sequence analysis;;Phylogeny analysis 中文刊名：甘肃农业科技 英文刊名：Gansu Agricultural Science and Technology 机构：甘肃省农业科学院作物研究所; 出版日期：2019-09-25 出版单位：甘肃农业科技 年：2019 期：09 基金：国家自然科学基金项目(31760426)资助 语种：中文; 页：37-42 页数：6 CN：62-1050/S ISSN：1001-1463 分类号：S563.2

摘要

COBRA基因编码糖基磷脂酰肌醇锚定蛋白,是次生壁加厚和纤维素含量的调节因子。亚麻纤维为韧皮纤维,纤维产量与次生壁中纤维素含量及次生壁加厚密切相关。通过BLASTP和Pfam分析,鉴定到24个亚麻COBRA家族成员,并对其进行基因结构、蛋白理化性质及系统进化等生物信息学分析。结果表明,有22个成员具有跨膜结构域,21个成员具有N-端信号肽,16个成员具有GPI锚定位点,所有蛋白均具有CCVS保守结构域而且都定位在细胞膜。基因结构和系统进化分析表明,亚麻COBRA基因家族分为2个亚家族:COBRA-I和COBRA-II,2个亚家族的基因结构具有明显的差异。
        The COBRA gene encodes the glycosylphosphatidylinositol anchoring protein,which is a regulator of secondary wall thickening and cellulose content. Flax fiber is bast fiber,and fiber yield is closely related to the content of cellulose in secondary wall and the thickening of secondary wall. Through BLASTP and Pfam analysis,24 members of the COBRA family were identified in this study, and the gene structure,physicochemical properties of proteins,systematic evolution were conducted. The results showed that 22 members had transmembrane domain,21 members had N-terminal signal peptide,16 members had GPI anchor site,and all proteins had CCVS conserved domain and were located in cell membrane. The results of gene structure analysis and systematic evolution showed that the COBRA gene family was divided into two subfamilies,COBRA-I and COBRA-II,the gene structure of the two subfamilies were significantly different. This study laid a foundation for the in-depth study on the function of COBRA gene and the use of COBRA gene to increase the fiber yield.

引文

[1] ZOHARY D. MONOPHYLETIC V S. Polyphyletic origin of the crops on which agriculture was founded in the near east[J]. Gen. Resour.Crop Evol.,1999(46):133-142.
    [2]张运晖,赵瑛,罗俊杰.甘肃胡麻产业发展浅议[J].甘肃农业科技,2013(7):54-55.
    [3] GREEN A G,CHEN Y,SINGH S P,et al.Flax. In Compendium of transgenic crop plants[D]. Oxford:BlackwellPublishing Ltd,2008:199-226.
    [4] WU J, ZHAO Q, ZHANG L, et al. QTL mapping of fiber-related traits based on a high-density genetic map in flax(Linum usitatissimum L.)[J]. Front. Plant. Sci., 2018(9):885.
    [5] BORNER G H,LILLEY K S,STEVENS T J,et al. Identification of glycosyl phosphatidylinositol-anchored proteins in Arabidopsis, a proteomic and genomic analysis[J]. Plant Physiol,2003(132):568-577.
    [6] BROWN, D M, ZEEF L A H, ELLIS J,et al. Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics[J]. Plant Cell, 2005(17):2281-2295.
    [7] SATO K,SUZUKI R,NISHIKUBO N,et al.Isolation of a novel cell wall architecture mutant of rice with defective Arabidopsis COBL4ortholog BC1 required for regulated deposition of secondary cell wall components[J]. Planta,2010(232):257-270.
    [8] ROUDIER F,SCHINDELMAN G,DESALLE R,et al. The COBRA family of putative GPIanchored proteins in Arabidopsis a new fellowship in expansion[J]. Plant Physiol., 2002(130):538-548.
    [9] CAO Y,TANG X F, GIOVANNONI J,et al.Functional characterization of a tomato COBRA-like gene functioning in fruit development and ripening[J]. BMC Plant Biol.,2012(12):211.
    [10] DAI X X,YOU C J,WANG L,et al. Molecular characterization,expression pattern,and function analysis of the OsBC1L family in rice[J]. Plant Mol. Biol.,2009(71):469-48119.
    [11] BRADY S M,SONG S,DHUGGA K S,et al. Combining expression and comparative evolutionary analysis. The COBRA gene family[J]. Plant Physiol.,2007(143):172-187.
    [12] YE X,KANG B G,OSBURN L D,et al.The COBRA gene family in populus and gene expression invegetative organs and in response to hormones and environmental stresses[J].Plant Growth Regul.,2009(58):211-223.