射频消融治疗兔VX2肾癌外周血细胞因子变化
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摘要
目的
通过背部小切口组织块种植法构建兔VX2肾癌模型,评价背部小切口组织块种植法构建兔VX2肾癌模型安全性和可靠性,行射频消融治疗后检测其外周血细胞因子浓度变化,探讨射频消融治疗肾脏肿瘤引起的免疫系统变化及机制。
方法
将20只新西兰大白兔,利用背部小切口组织块种植法构建兔VX2肾癌模型,2周后将实验兔处死,行尸检和病理学检查,明确兔背部小切口组织块种植法构建兔VX2肾癌模型的成功率,观察肿瘤生长情况和有无邻近脏器淋巴结转移。
将30只新西兰大白兔随机分为三组,分别为射频治疗组、手术治疗组、肿瘤对照组。构建成VX2肾癌模型后,射频治疗组、手术治疗组分别进行射频消融术、根治性肾脏切除术治疗肾脏肿瘤,而肿瘤对照组仅行对照处理后,分别术后7天、14天、28天检测三组实验兔外周血中的IFN-γ、TNF-α和白介素-2、6、10的浓度变化,观察射频消融术后对实验兔的免疫系统影响,行CT影像学检查,观察射频消融前后影像学变化。三组实验兔分别于术后7天、14天每组随机处死2只观察术后病理学变化。处理28天后,将剩余三组实验兔全部处死,行病理学检查,评价射频消融治疗有效性。
结果
20只新西兰大白兔VX2肾癌模型均构建成功,所有肿瘤均为单发,呈外生性生长,形状规则;未见肿瘤突破肾包膜,较为符合临床早期肾癌特征。
射频治疗组10只实验兔射频消融术后,病理检查未见明显肿瘤组织残留,CT扫描均显示肿瘤组织毁损完全,增强扫描未见强化。
射频治疗组同手术治疗组术后7天比较:外周血中的IFN-γ浓度升高,而IL-2、IL-6、TNF-α和IL-10浓度无明显差异;术后14天比较:外周血中的IL-6浓度降低,而IFN-γ浓度升高,IL-2、TNF-α和IL-10浓度无明显差异;术后28天比较:外周血中的IL-2和IFN-γ升高,IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
射频治疗组同肿瘤对照组术后7天比较:外周血中的IL-6和TNF-α降低,IFN-γ升高,而IL-2、IL-10浓度无明显差异;术后14天比较:外周血中的IL-2和IFN-γ升高, IL-6和IL-10下降,而TNF-α浓度无明显差异;术后28天比较:外周血中的IL-2和IFN-γ浓度升高,IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
手术治疗组同肿瘤对照组术后7天比较:外周血中IFN-γ、IL-10和IL-2浓度无明显差异,而TNF-α和IL-6降低;术后14天比较:外周血中IL-6和IL-10浓度降低,IFN-γ浓度升高,IL-2和TNF-α浓度无明显差异;术后28天比较:外周血中IL-2和IFN-γ浓度升高,而IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
结论
背部小切口组织块种植法构建兔VX2肾癌模型成功率较高,方法简单,肿瘤组织生物学稳定,易于操作,对实验兔损伤小,且耐受性良好,是较为理想的构建兔VX2肾癌模型方法。
射频消融治疗肾脏肿瘤不仅可以安全有效充分毁损肿瘤组织,同时对实验兔的免疫系统产生影响,可逆转因肿瘤引起免疫抑制,增强抗肿瘤免疫;表现为外周血的IFN-γ、IL-2含量增高, IL -6和IL-10含量降低;其机制可能是肿瘤组织毁损坏死后,肿瘤对机体免疫抑制作用降低,特别是IL -6和IL-10免疫抑制因子含量降低,导致机体免疫系统正常活化;同时由于毁损的肿瘤组织原位留置于体内,肿瘤毁损后大量肿瘤抗原释放,引起免疫系统活化,引起外周血中的细胞因子发生变化。射频消融治疗组外周血中IFN-γ、IL-2含量增高,而IL -6和IL-10含量降低,这些细胞因子的变化可调节免疫系统导致CD4 +T细胞,NK细胞数量增加和功能的增强,机体抗肿瘤免疫增强。
Objective
Building renal VX2 cancer model by VX2 tissue mass planting method with small incision of the back, Evaluating the safety and reliability of this method which we constructed renal VX2 cancer model .The rabbit with renal VX2 cancer were treated by radiofrequency ablation.After this operation ,checking its peripheral blood cytokines .This paper discusses the changes in the peripheral blood cytokines of rabbit with kidney VX2 cancer,after radiofrequency ablation.Exploring the radiofrequency ablation causing changes of immune system and mechanism , when kidney VX2 tumors were treated by radiofrequency ablation.
Methods
20 New Zealand white rabbits will be constructed VX2 kidney cancer model by VX2 tissue mass planting method with small incision of the back.After 2 weeks ,the 20 New Zealand white rabbits will be sacrificed, to do the experiment autopsy and pathology examination, get kidney model building success rate and observe whether they have adjacent organs transfer and lymph node metastasis. 30 New Zealand big white rabbits were randomly divided into three groups, respectively, radiofrequency ablation group, postoperative therapy and the tumor control groups. After VX2 kidney model constructed,they were namely given radiofrequency, Radical nephrectomy and control treatments . After 7 days, days 14 and 28 days,the three groups were monitored the IFN-γ、TNF-αand IL- 2, 6, 10 content changes in the peripheral blood. 28 days after Processing,every experimental animals were executed pathology examination and CT imaging examination, compare postoperative CT imaging changes. Meanwhile every group of experimental animals were random selected two rabbits ,then were executed,observing postoperative pathology changes,on postoperative 7 days, and 14 days.After 28 days, the remaining rabbits were executed, and done pathology examination, the evaluating the effectiveness of radiofrequency ablation therapy.
Results
20 New Zealand big white rabbits VX2 renal cancer models were constructed successfully, All tumors are sporadic,The tumors were more exophytic sporadic and appearance rules .We did not see the renal capsule tumor invasion, relatively matching clinical early kidney features. Tumor-burdened rabbits grows stability;
Radiofrequency ablation group of 10 rabbits were not found residual tumor tissue in pathological examination.
The CT scans showed completely damaged tumor tissue.
Radiofrequency treatment group compared with surgical treatment group Postoperative 7 days, IFN-γconcentration increases, and IL - 2, IL - 6, TNF–αand IL - 10 concentration were no obvious difference; Postoperative 14 days comparison: IL - 6 concentration was reduced, and the elevated IFN–γconcentration, IL - 2, TNF–αand IL - 10 concentration no obvious difference; Postoperative 28 days comparison: IL-2 and IFN–γincreases, IL-6 and IL-10 concentration lower,that TNF–αconcentration was no obvious difference.
Radiofrequency treatment group compared with tumor control group, Postoperative 7 days comparison: IL-6 and TNF-αwas reduced,IFN–γwas increased, IL-2 and IL-10 concentration were no difference; Postoperative 14 days comparison: IL - 2 and IFN–γrose, IL- 6, and IL-10 concentration were lower; Postoperative 28 days comparison: IL-2 and IFN-γconcentration increased, IL- 6 and IL-10 concentration decreased ,that TNF-αconcentration were no obvious difference.
Surgical treatment with tumor control group of seven days after comparison: IFN-γ, IL-10 and IL-2 concentration were no obvious difference, and TNF-αand IL - 6 decreased; 14 days comparison: IL - 6 and IL - 10 concentration decreased, IFN-γconcentration increased, IL - 2 and TNF-αconcentration were no obvious difference; Postoperative 28 days comparison: IL - 2 and IFN-γconcentration increased, and IL - 6 and IL - 10 concentration was lower ,that TNF-αconcentration was no difference.
Conclusion
The method of costrcuting rabbit kidney VX2 cancer mode by VX2 tissue mass planting method with small incision of the back is very ideal .It is simple, It has a high success rate and is biology stable model.
Radiofrequency ablation therapy can not only safely and effectively damages the tumor, but also cause immune system changes, and enhance the antitumor immune. It can cause IL– 2 and IFN-γcontent increased, IL - 6 and IL - 10 content low in the peripheral blood of experimental rabbit. Its mechanism may be the tumor tissue necrosis were fully damaged, immune inhibition were reduced, especially IL - 6 and IL-10 immunosuppression factor levels are low, It cause the immune system activation. At the same time because the damaged tumor tissue were left in body, tumor antigen releases, which causes the immune system activation. The cytokines in peripheral blood changes.The IFN -γ, IL-2 content increased, IL-6 and IL-10 content is reduced in the peripheral blood of radiofrequency ablation group, It cause strengthen function of CD4 + T cells, NK cell number and other lymphocy, organisms antineoplastic immune enhanced.
通过背部小切口组织块种植法构建兔VX2肾癌模型,评价背部小切口组织块种植法构建兔VX2肾癌模型安全性和可靠性,行射频消融治疗后检测其外周血细胞因子浓度变化,探讨射频消融治疗肾脏肿瘤引起的免疫系统变化及机制。
方法
将20只新西兰大白兔,利用背部小切口组织块种植法构建兔VX2肾癌模型,2周后将实验兔处死,行尸检和病理学检查,明确兔背部小切口组织块种植法构建兔VX2肾癌模型的成功率,观察肿瘤生长情况和有无邻近脏器淋巴结转移。
将30只新西兰大白兔随机分为三组,分别为射频治疗组、手术治疗组、肿瘤对照组。构建成VX2肾癌模型后,射频治疗组、手术治疗组分别进行射频消融术、根治性肾脏切除术治疗肾脏肿瘤,而肿瘤对照组仅行对照处理后,分别术后7天、14天、28天检测三组实验兔外周血中的IFN-γ、TNF-α和白介素-2、6、10的浓度变化,观察射频消融术后对实验兔的免疫系统影响,行CT影像学检查,观察射频消融前后影像学变化。三组实验兔分别于术后7天、14天每组随机处死2只观察术后病理学变化。处理28天后,将剩余三组实验兔全部处死,行病理学检查,评价射频消融治疗有效性。
结果
20只新西兰大白兔VX2肾癌模型均构建成功,所有肿瘤均为单发,呈外生性生长,形状规则;未见肿瘤突破肾包膜,较为符合临床早期肾癌特征。
射频治疗组10只实验兔射频消融术后,病理检查未见明显肿瘤组织残留,CT扫描均显示肿瘤组织毁损完全,增强扫描未见强化。
射频治疗组同手术治疗组术后7天比较:外周血中的IFN-γ浓度升高,而IL-2、IL-6、TNF-α和IL-10浓度无明显差异;术后14天比较:外周血中的IL-6浓度降低,而IFN-γ浓度升高,IL-2、TNF-α和IL-10浓度无明显差异;术后28天比较:外周血中的IL-2和IFN-γ升高,IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
射频治疗组同肿瘤对照组术后7天比较:外周血中的IL-6和TNF-α降低,IFN-γ升高,而IL-2、IL-10浓度无明显差异;术后14天比较:外周血中的IL-2和IFN-γ升高, IL-6和IL-10下降,而TNF-α浓度无明显差异;术后28天比较:外周血中的IL-2和IFN-γ浓度升高,IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
手术治疗组同肿瘤对照组术后7天比较:外周血中IFN-γ、IL-10和IL-2浓度无明显差异,而TNF-α和IL-6降低;术后14天比较:外周血中IL-6和IL-10浓度降低,IFN-γ浓度升高,IL-2和TNF-α浓度无明显差异;术后28天比较:外周血中IL-2和IFN-γ浓度升高,而IL-6和IL-10浓度降低,TNF-α浓度无明显差异。
结论
背部小切口组织块种植法构建兔VX2肾癌模型成功率较高,方法简单,肿瘤组织生物学稳定,易于操作,对实验兔损伤小,且耐受性良好,是较为理想的构建兔VX2肾癌模型方法。
射频消融治疗肾脏肿瘤不仅可以安全有效充分毁损肿瘤组织,同时对实验兔的免疫系统产生影响,可逆转因肿瘤引起免疫抑制,增强抗肿瘤免疫;表现为外周血的IFN-γ、IL-2含量增高, IL -6和IL-10含量降低;其机制可能是肿瘤组织毁损坏死后,肿瘤对机体免疫抑制作用降低,特别是IL -6和IL-10免疫抑制因子含量降低,导致机体免疫系统正常活化;同时由于毁损的肿瘤组织原位留置于体内,肿瘤毁损后大量肿瘤抗原释放,引起免疫系统活化,引起外周血中的细胞因子发生变化。射频消融治疗组外周血中IFN-γ、IL-2含量增高,而IL -6和IL-10含量降低,这些细胞因子的变化可调节免疫系统导致CD4 +T细胞,NK细胞数量增加和功能的增强,机体抗肿瘤免疫增强。
Objective
Building renal VX2 cancer model by VX2 tissue mass planting method with small incision of the back, Evaluating the safety and reliability of this method which we constructed renal VX2 cancer model .The rabbit with renal VX2 cancer were treated by radiofrequency ablation.After this operation ,checking its peripheral blood cytokines .This paper discusses the changes in the peripheral blood cytokines of rabbit with kidney VX2 cancer,after radiofrequency ablation.Exploring the radiofrequency ablation causing changes of immune system and mechanism , when kidney VX2 tumors were treated by radiofrequency ablation.
Methods
20 New Zealand white rabbits will be constructed VX2 kidney cancer model by VX2 tissue mass planting method with small incision of the back.After 2 weeks ,the 20 New Zealand white rabbits will be sacrificed, to do the experiment autopsy and pathology examination, get kidney model building success rate and observe whether they have adjacent organs transfer and lymph node metastasis. 30 New Zealand big white rabbits were randomly divided into three groups, respectively, radiofrequency ablation group, postoperative therapy and the tumor control groups. After VX2 kidney model constructed,they were namely given radiofrequency, Radical nephrectomy and control treatments . After 7 days, days 14 and 28 days,the three groups were monitored the IFN-γ、TNF-αand IL- 2, 6, 10 content changes in the peripheral blood. 28 days after Processing,every experimental animals were executed pathology examination and CT imaging examination, compare postoperative CT imaging changes. Meanwhile every group of experimental animals were random selected two rabbits ,then were executed,observing postoperative pathology changes,on postoperative 7 days, and 14 days.After 28 days, the remaining rabbits were executed, and done pathology examination, the evaluating the effectiveness of radiofrequency ablation therapy.
Results
20 New Zealand big white rabbits VX2 renal cancer models were constructed successfully, All tumors are sporadic,The tumors were more exophytic sporadic and appearance rules .We did not see the renal capsule tumor invasion, relatively matching clinical early kidney features. Tumor-burdened rabbits grows stability;
Radiofrequency ablation group of 10 rabbits were not found residual tumor tissue in pathological examination.
The CT scans showed completely damaged tumor tissue.
Radiofrequency treatment group compared with surgical treatment group Postoperative 7 days, IFN-γconcentration increases, and IL - 2, IL - 6, TNF–αand IL - 10 concentration were no obvious difference; Postoperative 14 days comparison: IL - 6 concentration was reduced, and the elevated IFN–γconcentration, IL - 2, TNF–αand IL - 10 concentration no obvious difference; Postoperative 28 days comparison: IL-2 and IFN–γincreases, IL-6 and IL-10 concentration lower,that TNF–αconcentration was no obvious difference.
Radiofrequency treatment group compared with tumor control group, Postoperative 7 days comparison: IL-6 and TNF-αwas reduced,IFN–γwas increased, IL-2 and IL-10 concentration were no difference; Postoperative 14 days comparison: IL - 2 and IFN–γrose, IL- 6, and IL-10 concentration were lower; Postoperative 28 days comparison: IL-2 and IFN-γconcentration increased, IL- 6 and IL-10 concentration decreased ,that TNF-αconcentration were no obvious difference.
Surgical treatment with tumor control group of seven days after comparison: IFN-γ, IL-10 and IL-2 concentration were no obvious difference, and TNF-αand IL - 6 decreased; 14 days comparison: IL - 6 and IL - 10 concentration decreased, IFN-γconcentration increased, IL - 2 and TNF-αconcentration were no obvious difference; Postoperative 28 days comparison: IL - 2 and IFN-γconcentration increased, and IL - 6 and IL - 10 concentration was lower ,that TNF-αconcentration was no difference.
Conclusion
The method of costrcuting rabbit kidney VX2 cancer mode by VX2 tissue mass planting method with small incision of the back is very ideal .It is simple, It has a high success rate and is biology stable model.
Radiofrequency ablation therapy can not only safely and effectively damages the tumor, but also cause immune system changes, and enhance the antitumor immune. It can cause IL– 2 and IFN-γcontent increased, IL - 6 and IL - 10 content low in the peripheral blood of experimental rabbit. Its mechanism may be the tumor tissue necrosis were fully damaged, immune inhibition were reduced, especially IL - 6 and IL-10 immunosuppression factor levels are low, It cause the immune system activation. At the same time because the damaged tumor tissue were left in body, tumor antigen releases, which causes the immune system activation. The cytokines in peripheral blood changes.The IFN -γ, IL-2 content increased, IL-6 and IL-10 content is reduced in the peripheral blood of radiofrequency ablation group, It cause strengthen function of CD4 + T cells, NK cell number and other lymphocy, organisms antineoplastic immune enhanced.
引文
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