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早熟禾厌氧发酵特性和产气潜力的研究
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摘要
我国是农业大国,农业废弃物种类繁多而且数量巨大,能够作为生物质能源加以开发利用的资源非常丰富,但以往大量的农业废弃物都被当成垃圾,造成了资源浪费和环境污染。利用厌氧发酵技术将其生物质资源转化为高品位沼气能源,具有技术成熟、转化效率高、应用方便等特点,可以有效地回收利用农业废弃物中物质和能量,是比较理想的农业废弃物回收系统。
     为了研究茎叶类农业废弃物的厌氧发酵特性,利用自行设计的厌氧发酵试验装置,在30±1℃恒温条件下,对茎叶类农业废弃物进行了厌氧发酵。通过对试验过程中重要指标的测定和比较,探究各发酵原料的特性,以寻求更多更适合的发酵原料,从而提高农业废弃物的应用价值。研究内容和结果主要包括以下几个方面:
     以早熟禾、佛手瓜茎叶和番茄茎叶三种茎叶类农业废弃物为原料,分别进行了厌氧发酵试验,并进行比较。结果表明:早熟禾累积产气量最高,且持续时间最长,佛手瓜茎叶次之,番茄茎叶最差,早熟禾、佛手瓜茎叶和番茄茎叶三者累积产气量分别为54830mL、45360mL、33090mL;在发酵过程中早熟禾和佛手瓜茎叶均出现一定程度的酸积累现象,但经过系统的自动调节,一段时间后均恢复正常,其中佛手瓜恢复的较慢;佛手瓜茎叶的甲烷含量较低,且产甲烷最不稳定,早熟禾和番茄茎叶甲烷含量较高;产气潜力方面,三者差异均达极显著水平。综合产气量、甲烷含量、产气潜力等指标的测定结果,早熟禾表现最好。
     进一步研究早熟禾的厌氧发酵特性,设置接种量10%、20%、30%和40%四个梯度,和发酵浓度4%、6%、8%和10%四个梯度分别进行比较试验,讨论接种量、发酵浓度对早熟禾产气量、甲烷含量、pH值的影响。结果表明:综合考虑,早熟禾接种量在30%、发酵浓度在6%~8%条件下,早熟禾厌氧发酵产气效果最好。
     将早熟禾和猪粪混合发酵,以累积产气量和平均甲烷含量为指标,选取碳氮比、发酵浓度和接种量三个因子进行多因素试验,变化范围分别为13~30,4~10%和10~40%。依据二次正交旋转组合设计的方法,建立因素对产气量和甲烷含量的回归方程,探讨碳氮比、发酵浓度和接种量对产气量和甲烷含量的影响规律。结果表明:影响产气量的主因素排序为接种量>发酵浓度>碳氮比;影响甲烷含量的主因素排序为接种量>碳氮比>发酵浓度;且各因素之间具有一定的交互作用,不同的两个因素相互影响对产气量和甲烷含量的影响也就不同;采用SAS数据处理系统对试验数据进行分析,可以得到早熟禾和猪粪混合发酵原料要获得较好产气量的最佳工艺是:碳氮比为23.5:1,发酵浓度为7.35%,接种量为29.02%,混合原料产气量理论值最高可达38.06L;要获得较高平均甲烷含量的最佳工艺是:碳氮比为23.64:1,发酵浓度为7.23%,接种量为30.12%,混合原料平均甲烷含量理论最高值可达54.79%。
China is a large agricultural country, and there are a variety of agricultural wastes, can be used as biomass energy development and utilization of resources is very rich, but usually a lot of agricultural wastes are as garbage, resulting in waste of resources and environmental pollution. So we can make use of anaerobic fermentation technology convert them into high-grade energy. Anaerobic fermentation system can effectively recover agricultural wastes, and it is an ideal system.
     In order to study and compare characteristics of anaerobic fermentation of agricultural waste, this paper uses independently designed anaerobic fermentation experiment installs ,and carries out a variety of anaerobic fermentation of agricultural waste at 30±1℃. Through the determination and comparison of key indicators the course of the experiment to explore the characteristics of the fermentation of raw materials, to find more and suitable raw materials for fermentation, there by enhancing the value of agricultural waste. Main research contents and results include the following:
     This paper studies three kinds of agricultural wastes (Bluegrass, stems and leaves of chayote and stems and leaves of tomato)as raw materials, anaerobic digestion tests were conducted and compared. The results show that: Bluegrass has longest gas production and the most cumulative gas production, stems and leaves of chayote is the second, stems and leaves of tomato is the worst. The cumulative production of bluegrass , stems and leaves of chayote and stems and leaves of tomato are 54830mL, 45360mL, 33090ml;Bluegiass and stems and leaves of chayote have a level of acid accumulation in the fermentation, but after the automatic adjustment of the system, they return to normal after a period of time, but stems and leaves of chayote recoveries slow, and its methane content is low, the methane content of bluegrass and stems and leaves of tomato is higher; Comparing gas production, methane, gas production potential and other indicators ,the bluegrass’synthetic performance is best.
     To further study the characteristics of anaerobic fermentation of bluegrass, this paper uses inoculation volume are 10%, 20%, 30% and 40% of the four gradient, and the fermentation concentration are 4%, 6%, 8% and 10% of the four gradient to do comparison tests, and discuss the affect of inoculation volume and fermentation concentration on gas production, methane and pH value. The results show that: When bluegrass’inoculation volume is 30%, fermentation concentration is 6%~8%, the effect of anaerobic fermentation is better.
     The bluegrass and pig manure are as raw materials of mixed fermentation .To put cumulative gas production and the average methane content as the index and select the C/N, fermentation density and inoculation volume three factors to do multi-factor tests, the three factors change in the range of 13 ~ 30,4 ~ 10% and 10 ~ 40%. Basing on the method of the two orthogonal revolving combination test, Regression equations of three factors on gas production and methane contents are built, and the influence law of how C/N, fermentation density and inoculation volume affect on gas production and methane contents is studied. The results show that: The main factors affecting gas production order of: the inoculation volume >the fermentation density >the C/N, the main factors affecting methane content order of: the inoculation volume >the C/N> the fermentation density .Various factors have some interaction, two different factors interact on gas production and methane content is different; Test data are analysed by using SAS data processing system, it can get the best technology of the raw materials (bluegrass and pig manure )of mixed to get better gas production: C/N is 23.06:1 , fermentation density is 7.35%, the inoculation volume is 29.02% , the theoretical value of mixed raw material’s gas production is up to 38.06L; the best technology that can get higher methane content is: C/N is 23.64:1, fermentation density is 7.23%, the inoculation volume is 30.12%, the theoretical value of mixed raw material’s methane content is up to 54.79%.
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