甘蓝型油菜基因组中控制种子维生素E含量QTL的检测和分析
摘要
维生素E又名生育酚,是重要的维生素之一,属于脂溶性维生素。具有强抗氧化性,在动物及植物生命中有着不可取代的重要地位。天然的维生素E主要来自油料作物的种子,由于油菜富含维生素E和其广大的种植面积,作为油料作物的油菜日渐成为研究的对象。
本实验室以甘蓝型冬油菜品种Tapidor和半冬性油菜品种Ningyou7亲本构建了TN DH群体,并在2年时间3个环境进行了种植。实验室还在TN DH群体基础上衍生了RC-F_2群体,并在荆州环境下与TN DH群体一起种植。本研究结合文献,针对可能影响维生素E检测和合成的因素进行了初步的探讨。首先完善了测量方法中存在的不足——正己烷的挥发带来的实验误差。其次,发现环境对维生素E各组分的影响效应是不一样的。α-生育酚和γ-生育酚的含量比总生育酚含量更敏感于环境的变化。将同一种植环境的两个群体比较发现,RC-F_2群体表现出杂种优势。本研究还比较了甘蓝型和白菜型的两个种质资源种子中维生素E合成情况,发现白菜型油菜种子中的α-生育酚含量远远低于甘蓝型油菜种子中的α-生育酚含量。
本研究以实验室构建的TN图谱为基础,采用QTL分析软件WinQTLcart2.5,QTL作图软件QTLmapper2.0对种子中维生素E的各性状进行QTL定位及互作位点的检测和遗传效应分析。三个环境下的两个群体在p=0.05水平上共检测到54个影响种子中维生素E含量的QTL,通过BioMercator-2.1软件的整合得到25个独立的控制油菜种子维生素E含量的QTL。针对环境和群体中表达稳定的QTL——QtcA07-1,一个主要控制种子中α-生育酚含量的QTL,构建了BC_4F_2群体。利用BC_4F_2群体,不仅验证了QtcA07-1在TN群体中的存在,而且缩短了QtcA07-1的置信区间,提高了Ningyou7背景的纯度。另一方面利用拟南芥维生素E合成代谢途径上的酶基因序列信息,根据Hpt1酶基因的序列设计标记引物Hpt1,将标记Hpt1定位在QtcA07-1置信区间内。
Vitamin E is one of the important micronutrient,it comprises a distinctive group of potent antioxidant compounds,and it is also called tocopherol.As its strong anti-oxidation,it becomes an essential element of animals and plants.Rapeseed is one of oil seed which are rich in vitamin E.Rapeseed oil as an edible oil is very population in China,in addition,its extensive employment in European's transportation,which calls scientists to improving the quality of oil,and increasing vitamin E content in the rapeseed develops one scientific research direction.
The winter-typed B.napus variety Tapidor and the semi-winter typed variety Ningyou7 are two parents.A DH population named with TN DH population was constructed with the two varieties,and a RC-F_2 population was constructed based on TN DH population in our lab.TN DH population was planted in three environments during two years,while RC-F_2 population was co-grew with the TN DH population under the environment in Jingzhou.Considering these factors influencing synthesis of vitamin E in rape seed,we improved the measurement firstly,taking the volatilization of hexane into account.Secondly,we found that different tocopherol composition was influenced by environment factor,α-tocopherol andγ-tocopherol showed much more sensitive to environment factor than total tocopherol did.Under the same environment,RC-F_2 population performed better than TN DH population about tocopherol content in the seed. This study also analysed two Brassica germplast population's vitamin E content,α-tocopherol content detected in Brassica rapa was much less than in Brassica napus.
Combined TN map information,with QTL analysis software WinQTLcart2.5, QTLmapper2.0,there were 54 QTL(p=0.05) detected with the two populations.After integration with BioMercator-2.1 software,there still were 25 QTL,and 5 interaction loci pairs were detected.Asα-tocopherol has high activity,the QTL controllingα-tocopherol content—QtcA07-1 was selected,according to the information of QtcA07-1,BC_4F_2 was built up.With BC_4F_2,QtcA07-1 was confirmed,and the confidence interval was shortened,and the contribution from QtcA07-1 was enhanced.At the same time,Hpt1 enzyme belongs to the metabolism pathway of tocopherol,it was designed as a marker, and have been located in QtcA07-1.However,the result did not bring any more change except shortened QTL confident interval.
本实验室以甘蓝型冬油菜品种Tapidor和半冬性油菜品种Ningyou7亲本构建了TN DH群体,并在2年时间3个环境进行了种植。实验室还在TN DH群体基础上衍生了RC-F_2群体,并在荆州环境下与TN DH群体一起种植。本研究结合文献,针对可能影响维生素E检测和合成的因素进行了初步的探讨。首先完善了测量方法中存在的不足——正己烷的挥发带来的实验误差。其次,发现环境对维生素E各组分的影响效应是不一样的。α-生育酚和γ-生育酚的含量比总生育酚含量更敏感于环境的变化。将同一种植环境的两个群体比较发现,RC-F_2群体表现出杂种优势。本研究还比较了甘蓝型和白菜型的两个种质资源种子中维生素E合成情况,发现白菜型油菜种子中的α-生育酚含量远远低于甘蓝型油菜种子中的α-生育酚含量。
本研究以实验室构建的TN图谱为基础,采用QTL分析软件WinQTLcart2.5,QTL作图软件QTLmapper2.0对种子中维生素E的各性状进行QTL定位及互作位点的检测和遗传效应分析。三个环境下的两个群体在p=0.05水平上共检测到54个影响种子中维生素E含量的QTL,通过BioMercator-2.1软件的整合得到25个独立的控制油菜种子维生素E含量的QTL。针对环境和群体中表达稳定的QTL——QtcA07-1,一个主要控制种子中α-生育酚含量的QTL,构建了BC_4F_2群体。利用BC_4F_2群体,不仅验证了QtcA07-1在TN群体中的存在,而且缩短了QtcA07-1的置信区间,提高了Ningyou7背景的纯度。另一方面利用拟南芥维生素E合成代谢途径上的酶基因序列信息,根据Hpt1酶基因的序列设计标记引物Hpt1,将标记Hpt1定位在QtcA07-1置信区间内。
Vitamin E is one of the important micronutrient,it comprises a distinctive group of potent antioxidant compounds,and it is also called tocopherol.As its strong anti-oxidation,it becomes an essential element of animals and plants.Rapeseed is one of oil seed which are rich in vitamin E.Rapeseed oil as an edible oil is very population in China,in addition,its extensive employment in European's transportation,which calls scientists to improving the quality of oil,and increasing vitamin E content in the rapeseed develops one scientific research direction.
The winter-typed B.napus variety Tapidor and the semi-winter typed variety Ningyou7 are two parents.A DH population named with TN DH population was constructed with the two varieties,and a RC-F_2 population was constructed based on TN DH population in our lab.TN DH population was planted in three environments during two years,while RC-F_2 population was co-grew with the TN DH population under the environment in Jingzhou.Considering these factors influencing synthesis of vitamin E in rape seed,we improved the measurement firstly,taking the volatilization of hexane into account.Secondly,we found that different tocopherol composition was influenced by environment factor,α-tocopherol andγ-tocopherol showed much more sensitive to environment factor than total tocopherol did.Under the same environment,RC-F_2 population performed better than TN DH population about tocopherol content in the seed. This study also analysed two Brassica germplast population's vitamin E content,α-tocopherol content detected in Brassica rapa was much less than in Brassica napus.
Combined TN map information,with QTL analysis software WinQTLcart2.5, QTLmapper2.0,there were 54 QTL(p=0.05) detected with the two populations.After integration with BioMercator-2.1 software,there still were 25 QTL,and 5 interaction loci pairs were detected.Asα-tocopherol has high activity,the QTL controllingα-tocopherol content—QtcA07-1 was selected,according to the information of QtcA07-1,BC_4F_2 was built up.With BC_4F_2,QtcA07-1 was confirmed,and the confidence interval was shortened,and the contribution from QtcA07-1 was enhanced.At the same time,Hpt1 enzyme belongs to the metabolism pathway of tocopherol,it was designed as a marker, and have been located in QtcA07-1.However,the result did not bring any more change except shortened QTL confident interval.
引文
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2.丁效华.作物数量性状基因图位克隆研究进展.植物遗传资源学报,2005,6:464-468.
3.贺道华,张献龙.数量性状由表型变异到基因发现的研究进展.遗传,2006,28:1613-1618.
4.胡英考.植物维生素E合成及其生物技术改良.中国生物工程杂志,2004,24:32-35.
5.黄智明,翁海波,席宇,韩绍印,王时征.转入HPT1基因的油菜种子中维生素E含量的提高.植物生理学通讯,2006,42:888-890.
6.蒋守华,刘葛山,徐美琴.油料作物维生素E含量的研究进展.安徽农业科学,2007,35:5042-5043.
7.李静,王之盛.储藏中微量元素对维生素E稳定性的影响.饲料工业,2001,22:17-18.
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