山东省猪繁殖与呼吸障碍综合征流行病学调查研究
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摘要
猪繁殖与呼吸障碍综合征(Porcine reproductive and respiratory syndrome,PRRS)是造成目前养猪业严重经济损失的重要疾病之一。其病原是猪繁殖与呼吸障碍综合征病毒(Porcinereproductive and respiratory virus,PRRSV),该病毒在临床上引起母猪流产、早产、死胎、断奶仔猪肺炎,生长迟缓及其他病原的继发感染。因此该研究调查了该病在山东的流行状况,对其分布及其影响传播的因素进行系统的研究。
本文通过设计外侧引物F1、R1,扩增大小约400bp的片段,内侧引物F2、R2,扩增大小约170bp的片段,建立PRRSV的套式PCR检测方法;应用该方法对PRRSV、猪伪狂犬病病毒(PRV)、圆环病毒Ⅱ型(PCV-Ⅱ)、猪瘟病毒(CSFV)及空白对照进行检测。结果表明,除PRRSV外,猪伪狂犬病病毒(PRV)、圆环病毒Ⅱ型(PCV-Ⅱ)、猪瘟病毒(CSFV)及空白对照的检测结果均为阴性;将提取的RNA依次稀释,结果RT-PCR可以扩增出10-4稀释度的病毒核酸,而RT-nestPCR可以扩增出10-7稀释度的病毒核酸,后者的敏感性提高了1000倍。表明该研究建立的RT-nestPCR检测方法具有特异性强、敏感性高、重现性好等优点。
对山东省进行了PRRS的流行病学问卷调查。结果显示PRRS多发生于冬、春季节及每年的7、8月份;小规模养殖场及散养户多发;发病猪的年龄主要为30~70日龄,其次为2~30日龄,怀孕母猪占17%,其他占3%;临床症状表现母猪繁殖障碍的占30%,表现仔猪呼吸道症状的占50%,母猪繁殖障碍、仔猪呼吸道症状的占30%,其他临床表现的占10%。应用本研究建立的PRRS套式PCR对送检样品进行检测。结果显示,其中15个县市检出PRRSV,阳性检出率为12.5%~100%,平均阳性率为34.4%。以上结果表明PRRS已成为危害当地养猪业的重要疫病。
The porcine reproductive and respiratory syndrome(PRRS) is one of the causative disease which has risen humorous economic loss on swine industry.It’s pathogeny is the porcine reproductive and respiratory syndrome virus (PRRSV).The disease is characterized by reproductive troubles such as abortions farrowing or weak piglets, preweaning pislets pneum onia mortality and mortality in sows.To investigate the prevalence station,it’s distribution,occurrence,spread approach inShandong Province, so we research the epidemiology of Porcine Reproduction and Respiratory Syndrome in Shan Dong.
This syudy designed the outer pair of primers F1 R1, a 400bp products was obtained and the inner pair of primers F2 R2,a 170bp was amplified to establish a nested PCR assay, were used to detect PRRSV PRV PCV-2 CSFV and negative control.The results showed that except for PRRSV, other detect results are negative;RNA was extracted and diluted 10-1~10-4.A visible objictive strip can be amplified still.This indicated that the established nested PCR assay has the virtues of stronger speciation,higher sensitivity and well stability.
The epidemiological investigation was carried out by questionnaire in Shan Dong Province.The investigation results showed that immunity hogpen still.The PRRS often occur in the Spring and Winter,and in July and Augest of each year.The breed type of disease swine is small scale breed hogpen and individual breed. Clinical of PRRS characterized only by sow reproductive troubles is 30%;Piglets respiratory symptom is 50%;sow reproductive problems-Piglets respiratory symptom is 30%;preweaning syndrome is 40%;other clinical symptom is 10%.The samples were detected,the resultshowed that the sample had the positive;the positive ratio is 12.5%~100%,the average positive ratio 34.4%.This indicated that the PRRS has been a important disease which damages to swine industry.
本文通过设计外侧引物F1、R1,扩增大小约400bp的片段,内侧引物F2、R2,扩增大小约170bp的片段,建立PRRSV的套式PCR检测方法;应用该方法对PRRSV、猪伪狂犬病病毒(PRV)、圆环病毒Ⅱ型(PCV-Ⅱ)、猪瘟病毒(CSFV)及空白对照进行检测。结果表明,除PRRSV外,猪伪狂犬病病毒(PRV)、圆环病毒Ⅱ型(PCV-Ⅱ)、猪瘟病毒(CSFV)及空白对照的检测结果均为阴性;将提取的RNA依次稀释,结果RT-PCR可以扩增出10-4稀释度的病毒核酸,而RT-nestPCR可以扩增出10-7稀释度的病毒核酸,后者的敏感性提高了1000倍。表明该研究建立的RT-nestPCR检测方法具有特异性强、敏感性高、重现性好等优点。
对山东省进行了PRRS的流行病学问卷调查。结果显示PRRS多发生于冬、春季节及每年的7、8月份;小规模养殖场及散养户多发;发病猪的年龄主要为30~70日龄,其次为2~30日龄,怀孕母猪占17%,其他占3%;临床症状表现母猪繁殖障碍的占30%,表现仔猪呼吸道症状的占50%,母猪繁殖障碍、仔猪呼吸道症状的占30%,其他临床表现的占10%。应用本研究建立的PRRS套式PCR对送检样品进行检测。结果显示,其中15个县市检出PRRSV,阳性检出率为12.5%~100%,平均阳性率为34.4%。以上结果表明PRRS已成为危害当地养猪业的重要疫病。
The porcine reproductive and respiratory syndrome(PRRS) is one of the causative disease which has risen humorous economic loss on swine industry.It’s pathogeny is the porcine reproductive and respiratory syndrome virus (PRRSV).The disease is characterized by reproductive troubles such as abortions farrowing or weak piglets, preweaning pislets pneum onia mortality and mortality in sows.To investigate the prevalence station,it’s distribution,occurrence,spread approach inShandong Province, so we research the epidemiology of Porcine Reproduction and Respiratory Syndrome in Shan Dong.
This syudy designed the outer pair of primers F1 R1, a 400bp products was obtained and the inner pair of primers F2 R2,a 170bp was amplified to establish a nested PCR assay, were used to detect PRRSV PRV PCV-2 CSFV and negative control.The results showed that except for PRRSV, other detect results are negative;RNA was extracted and diluted 10-1~10-4.A visible objictive strip can be amplified still.This indicated that the established nested PCR assay has the virtues of stronger speciation,higher sensitivity and well stability.
The epidemiological investigation was carried out by questionnaire in Shan Dong Province.The investigation results showed that immunity hogpen still.The PRRS often occur in the Spring and Winter,and in July and Augest of each year.The breed type of disease swine is small scale breed hogpen and individual breed. Clinical of PRRS characterized only by sow reproductive troubles is 30%;Piglets respiratory symptom is 50%;sow reproductive problems-Piglets respiratory symptom is 30%;preweaning syndrome is 40%;other clinical symptom is 10%.The samples were detected,the resultshowed that the sample had the positive;the positive ratio is 12.5%~100%,the average positive ratio 34.4%.This indicated that the PRRS has been a important disease which damages to swine industry.
引文
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