用户名: 密码: 验证码:
氨基酸螯合锌对肉仔鸡生长代谢的影响
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本研究通过两个试验,探索了锌源与锌水平对肉仔鸡生产性能、免疫机能、组织锌沉积以及营养物质代谢相关指标的影响,旨在研究不同锌源及其添加量对肉用仔鸡的营养效应,从而为肉仔鸡实用日粮中锌适宜添加量的确定提供科学依据。
     试验一氨基酸螯合锌对肉仔鸡生长性能和免疫功能的影响
     本试验采用单因子和2×4二因子组合完全随机试验设计,研究日粮锌来源及锌水平对肉仔鸡生产性能与免疫机能的影响。选取体重相近的2周龄AA肉鸡(公雏)270只随机分为9个处理,每组设5个重复,每个重复6只鸡。对照组饲喂锌含量为14.97mg/kg的半纯合基础日粮;试验1至4组分别在基础日粮中添加ZnSO_4·7H_2O(分析纯)20、40、60和80mg/kg,试验5至8组分别在基础日粮中添加氨基酸螯合锌(Zn-AA) 20、40、60和80mg/kg。试验25日龄结束。结果表明,不同的锌水平对F/G影响显著(P<0.05),添加60和80mg/kgZn时,饲料转化率较低锌组显著提高(p<0.05)。锌源对生产性能无显著影响,但显著影响肉仔鸡血清ANAE活性和ND抗体滴度(P<0.05),其中氨基酸螯合锌添加组显著高于硫酸锌组。锌添加水平显著影响ANAE活性和血清ND抗体滴度(P<0.05),并呈随添加水平提高而增高的趋势,其中添加60和80mg/kg组显著高于其它各组(P<0.05)。由此可见,在缺锌日粮中补充有机和无机锌均能改善肉仔鸡的生长性能和免疫功能,但氨基酸螯合锌改善肉仔鸡免疫功能的效果优于无机锌。综合生长和免疫指标,14~25日龄肉仔鸡适宜的锌水平在70mg/kg以上。
     试验二氨基酸螯合锌对肉仔鸡组织锌沉积量和物质代谢的影响
     本试验采用单因子和2×4二因子组合完全随机试验设计,研究日粮锌来源及锌水平对肉仔鸡组织锌沉积量和物质代谢的影响。选取体重相近的2周龄AA肉鸡(公雏)270只随机分为9个处理,每组设5个重复,每个重复6只鸡。对照组饲喂锌含量为14.97mg/kg的半纯合基础日粮;试验1至4组分别在基础日粮中添加ZnSO_4·7H_2O(分析纯)20、40、60和80mg/kg,试验5至8组分别在基础日粮中添加氨基酸螯合锌(Zn-AA) 20、40、60和80mg/kg。试验25日龄结束。结果表明,锌源对胫骨和胰腺锌沉积量影响显著,其中Zn-AA组显著高于硫酸锌组(P<0.05);锌添加水平显著影响胫骨、胰腺和血清锌含量(P<0.01),呈随Zn添加水平提高而增高的趋势,其中80mg/kg组胰腺和血清锌含量最高,20mg/kg组最低,与其他各水平组差异显著(P<0.05)。胫骨锌呈相似的变化规律,但除20mg/kg组外,其它各组间差异不显著。锌源对肉仔鸡血糖及血清总蛋白含量有极显著的影响(P<0.01),Zn-AA组显著高于ZnSO4组。锌添加水平对二者也有显著影响,其中60mg/kg组血糖最高,显著高于其他水平组(P<0.05),20mg/kg组总蛋白最低,显著低于其它各组。由此可见,日粮Zn水平直接影响肉仔鸡组织锌储备,其中氨基酸螯合锌具有更高的组织沉积效率。此外,日粮Zn水平对糖代谢和蛋白质代谢都有影响,尤以氨基酸螯合锌的作用更为明显。
The objective of this study was to evaluate the effects of different zinc sources and zinc levels on performance, immune function, zinc deposition and nutrient metabolism of broiler chicks. It can provide scientific basis for zinc level in practical diet of broiler chicks on the basis of comparing those different zinc sources.
     Experiment 1. The Effects of Zinc-Amino Acid Chelate on Performance and Immune Function of Broiler chicks
     The effects of different zinc sources and zinc levels on performance and immunity function of broiler chicks at 2 week-old were studied. A single factor and a 2*4 factorial arrangement of treatments in a completely random design was conducted. 270 male chicks were divided into 9 treatments (5 duplicates per treatment and each duplicate had 6 chicks) in random. The Control Group was fed semi-purified basic diet with 14.97mg/kg of zinc content. The Group 1 to 4 were added zinc-amino acid chelate(Zn-AA)at 20, 40, 60 and 80mg/kg. The Group 5 to 8 were added ZnSO_4·7H_2O at the same level as Zn-AA .The whole experiment was ended at 25 day.
     The result showed that: Feed gain ratio (F/G) was affected with dietary zinc levels significantly ( P<0.05). F/G of 60 and 80mg/kg zinc level groups were significantly higher than the groups which added zinc at lower level (P<0.05). There was no significant difference of performance among zinc sources groups, while ANAE activity and Serum ND antibody titer were affected with zinc sources, those two indexes of Zn-AA groups were higher than those of ZnSO4 groups (P<0.05). Zinc levels affected ANAE activity and Serum ND antibody titer significantly (P<0.05), and have a trend that those two indexes were increased with zinc levels were raised, those indexes of 60 and 80mg/kg zinc levels groups were higher than others significantly (P<0.05). It showed that adding organic and inorganic zinc in zinc deficiency diet can both improve performance and immune function of broiler chicks, and the effect of Zn-AA on improving immune function of broiler chicks was superior to inorganic zinc. Colligate the performance and immune indexes, the optimal zinc level in 14~25 day-old broiler chick was higher than 70mg/kg.
     Experiment 2. The Effects of Zinc-Amino Acid Chelate on tissue deposition rate and nutrient metabolism of Broiler chicks
     The effects of different zinc sources and zinc levels on tissue deposition rate and nutrient metabolism of broilers at 2 week-old were studied. A single factor and a 2*4 factorial arrangement of treatments in a completely random design was conducted. 270 male chicks were divided into 9 treatments (5 duplicates per treatment and each duplicate had 6 chicks) in random. The Control Group was fed semi-purified basic diet with 14.97mg/kg of zinc content. The Group 1 to 4 were added zinc-amino acid chelate(Zn-AA)at 20, 40, 60 and 80mg/kg. The Group 5 to 8 were added ZnSO_4·7H_2O at the same level as Zn-AA .The whole experiment was ended at 25 day.
     The result showed that: The effect of zinc sources on zinc deposition in tibia and pancreas were significantly, and the deposition of Zn-AA group higher than that of ZnSO4 group (P<0.05). Zinc levels effected zinc content in tibia, pancreas and serum significantly (P<0.01), and have a trend that those contents were increased with zinc levels were raised, the zinc content in pancreas and serum in 80mg/kg zinc level group were highest, and the zinc content in pancreas and serum in 20 mg/kg zinc level group were the lowest, and those content in the two zinc levels groups were different form others groups significantly (P<0.05). Zinc content in tibia had similar variety law, the difference between groups except 20mg/kg zinc level group was no significant (P>0.05).Zinc sources significantly influenced content of blood glucose and serum total protein (P<0.01), and those indexes of Zn-AA group higher than ZnSO4 group. Zinc level also had significant effect on content of blood glucose and serum total protein, the content of blood glucose in 60mg/kg zinc level was higher than others groups significantly (P<0.05), and the content of serum total protein in 20mg/kg zinc level group was lower than others groups significantly (P<0.05). It showed that dietary zinc level influence tissue zinc deposition of broiler chicks directly, and Zn-AA had higher tissue zinc deposition efficiency. Besides, dietary zinc level influenced on glucose metabolism and protein metabolism, especially as Zn-AA.
引文
[1]刘英丽.不同锌源生物利用率的研究[J].中国畜牧兽医. 2005,11 (32):5~7
    [2]李聚才,张春珍,庞琪艳,冯乐天.动物微量元素锌营养研究进展[J].宁夏农林科技. 2007,6:30~32
    [3]金锋.微量元素锌营养[J].微量元素与健康研究. 2006,2(23):61~63
    [4]郑文波,王振来,范嵋君,催破成.动物缺锌的研究进展[J].河北畜牧兽医. 1998, 13(4):215~217
    [5]罗晓花,孙新文,张仁平,柳广芝.微量元素锌的功能研究及机制[J].饲料世界. 2006, 9、10:9~12
    [6] Walter Mertz,朱莲珍(主校译). Trace elements in human and animal nutrition(人和动物的微量元素营养)[M].青岛出版社, 1994
    [7]王志武,孙建钢,孙锐锋,等.微量元素锌的生物学功能及其应用进展[J].饲料研究. 2005,8:12~16
    [8]常巧玲,孙建义.锌的生物学功能及有机锌的应用研究[J].中国动物保健. 2005,9:46~48
    [9]庞全海,张莉,张春有,杨旭华,卢俊彦.微量元素锌在动物健康及营养中的研究进展[J].动物医学进展,2002,23(2):41~43
    [10]韩新燕,郭勇.微量元素锌与动物营养[J].饲料博览. 2000,10:14~15
    [11]高林.不同锌源和锌水平日粮对桃源鸡生产性能及血液生化指标的影响研究[D],湖南农业大学农业推广硕士学位论文,2007
    [12]刘英丽.肉仔鸡不同锌源、铜源生物利用率的研究[D],华中农业大学硕士学位论文, 2004
    [13]董晓慧,杨原志.影响锌吸收、利用的因素[J].饲料工业,2002,9 (23):18~21
    [14]彭西,崔恒敏.禽类缺锌症的研究进展[J].山东家禽. 2002,8:45~47
    [15] Adham N F,Song M K. Effect of calcium and copper on zinc absorption in the rat [J]. Nutrition Metabolism.1980,24(5):281~290
    [16]李杰,许振英.饲粮植酸和锌水平对肉鸡锌吸收利用的影响[J].中国畜牧杂志1994,30 (5):3~4
    [17] Mohanna C., Nys Y. Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens [J]. British Poultry Science. 1999,40(1):108~114
    [18]王安,单安山,关湛铭.肉仔鸡微量元素添加剂配方生产效果的验证试验[J].饲料博览. 1989,2:1~4
    [19] Bartlett J R,Smith M O. Effects of different levels of zinc on the performance and immune competence of broilers under heat stress [J]. Poultry Science. 2003,82(10):1580~1588.
    [20]刘素杰,于德强,肖秀娟.饲粮中锌与维生素A水平对肉仔鸡生产性能的影响[J].饲料工业. 2006,27(6):47~50
    [21]陈克嶙,郭荣富,郭亚东.实用饲粮补锌对肉鸡组织锌、免疫器官及生产性能的影响[J].畜牧与兽医. 1998,4 (30):155~157
    [22]闫素梅,郝永清,史彬林,侯先志,骆丽芝.日粮锌水平对肉仔鸡组织锌浓度及其生产性能与免疫机能的影响[J].饲料工业. 2002,23:25~27
    [23]张桂芝,黄保华,石天红,等.锌在蛋雏鸡上的应用效果研究[J].山东农业科学. 2001,5:36~37
    [24]李魁,王梅玉,吴平格,顾长泰.不同锌剂的补锌效果研究[J].中国饲料. 1995,1:16~17
    [25]黄艳玲.肉仔鸡实用饲粮中锌适宜水平及有机锌源相对生物学利用率研究[D],中国农业科学院博士论文,2007.6
    [26]吕武兴.肉仔鸡微量矿物元素营养研究进展[J].湖南畜牧兽医. 2004,2:1~3
    [27]孙海霞,石宝明.微量元素-氨基酸螯合物在动物生产中的研究与应用功效[J].河北农业大学学报. 2003,26 (5):173~176
    [28]吴胜华,李吕木.有机微量元素研究与应用进展[J].农产品加工,2007,12:56~59
    [29] Coffey R. D., Cromwell G. L., Monegue H.J. Efficacy of a copper-lysine complex as a growth promotant for weanling pigs [J]. Journal Animal Science. 1994,72:2880~2886
    [30]王敏奇,许梓荣.饲粮中添加锌对仔猪不同组织微量元素沉积的影响[J].浙江大学学报(农业与生命科学版),2002,28 (5):559~564,
    [31] Cousis R.J. Regulatory aspects of zinc metabolism in liver and intestine[J].Nutr.Rev.37:97
    [32] Aoyagi S J, Baker D H. Nutritional evaluation of copper-lysine and zinc-lysine complexes for chicks[J]. Poultry Science. 1993,72:165~171
    [33]高秀萍.肉鸡对蛋氨酸螯合铜、铁、锰、锌生物学利用率的研究[J].山东农业科学. 1995,3:14
    [34]周桂莲,林映才蒋宗勇,蒋守群,余德谦.蛋氨酸螯合锌在黄羽肉鸡生产中的应用研究[J],饲料工业. 2004,25 (7):11~14
    [35]郭荣富,等.有机锌源对肉鸡生长性能和胫骨软骨症的影响[J].中国家禽. 2000,22(4):12~13
    [36]苏振刚.氨基酸螯合锌对肉鸡生物学效价及对生产性能和免疫功能的影晌[D],四川农业大学硕士学位论文,2004
    [37]孙德成,王守清,赵志恭,王文元.微量元素氨基酸螯合物对产蛋鸡生产性能的影响[J].中国饲料. 1995,1:14~15
    [38]刘瑞生,刘秀清.微量元素氨基酸螯合物在畜牧业上的研究概况[J].畜牧兽医科技信息. 2003,9:59~60
    [39] Kidd M T, Qureshi M A, Ferket P R, et al. Blood clearance of Escherichia coli and evaluation of mononuclear-phagocytic system as turkeys[J]. Poultry Science. 1994,73:1381~1389
    [40] Hahn.J.D, D.H.Baker. Growth and Plasma zinc responses of young pigs fed pharmacologic levels of zinc[J]. J. Anim. Sci. 1993,71:3020.
    [41] Van Heugetn E, Spears J W, Kegley E B, et al. Effects of organic forms of zinc on growth performance, tissue zinc distribution, and immune response of weanling pigs[J]. J. Anim. Sci.2003,81:2063~2071
    [42]陈洪亮,李德发,邢建军,王纪谭,李斌.蛋氨酸内络合锌对断奶仔猪生产性能的影响[J].中国饲料. 2001,1:21~22
    [43]刘瑞生.微量元素氨基酸鳌合物在养猪业上的研究概况[J].当代畜禽养殖. 2003,2:41~44
    [44] J. Kessler, I. Morel, P.A. Dufey, A. Gutzwiller, A. Stern and H. Geyer. Effect of organic zinc sources on performance, zinc status and carcass, meat and claw quality in fattening bulls [J]. Livestock Prod. Sci. 2003,81:161~171.
    [45]苏纯阳,董仲华,香红星.微量元素氨基酸(小肽)螯合物的研究应用进展[J].饲料工业. 2002(1):15~18
    [46]邵建华,陆腾甲.复合氨基酸微量元素螯合物饲料添加剂的应用与开发[J].饲料工业. 2000,21 (5):41~42
    [47]韩友文,滕冰.微量元素赖氨酸螯合物对生长育肥猪的饲养效果[J].饲料博览. 2000, 3:18~20
    [48] Wedekind K J. Methodology for assessing Zinc bioavailability: efficacy estimates for methionine, zinc sulfate, and zinc oxide [J]. J Animal Science. 1992,70(l):176~187.
    [49]罗俊寿,孙大华.微量元素锌对机体的影响作用[J].现代医药卫生. 2006,22(3):357~358
    [50]闫立新,李华周,刘军,黄永中,韩永利,刘迎鹏.不同锌源对雏鸡的生物学效价[J].山西农业大学学报. 1999,19(3):249~252
    [51]虞泽鹏,吴晋强.不同锌源对肉用仔鸡生产性能及部分生理生化指标的影响[J].饲料研究. 2002,12:1~3
    [52]马文强,冯杰,李奎.氨基酸螯合锌在养猪生产中的应用[J].饲料研究. 2007,5:40~42
    [53] Roberson R.H., Schaible, P. J. Zinc Requirement of the Chick [J]. Poul. Sci. 1985,37:1321
    [54]赛尔山别克·阿布力达,阿布都拉·肉孜,雒秋江,等.添喂蛋氨酸螯合锌对肉仔鸡消化代谢和生长的影响[J].动物营养学报. 1998,10(3):601
    [55]邓灶福.蛋氨酸螯合锌不同添加量对肉用仔鸡生产性能的影响[J].畜禽业. 2006,1:20~21
    [56]葛桂花.蛋氨酸螯合锌对雏鸡的营养效果及内源锌排出量的测定[D].东北农业大学. 1992.
    [57]彭茂兰,涂建军.锌与免疫功能[J].中国民康医学.2007,19(12):1121
    [58]宋福行,焦念志.锌离子对肽酶和碱性磷酸酶活性影响的初步研究[J].海洋科学. 2003,27,27 (3):64~65
    [59]周立波,孙淑滨,景慎文.缺锌对孕鼠生长发育及其肝脏、胎盘中谷胱甘肽过氧化物酶及过氧化氢酶活性的影响[J].山东医药. 2002,42 (36):1~3
    [60]吕广宙,陆治年,丁晓明.低锌日粮补锌对断奶前后犊牛免疫机能的影响[J].畜牧兽医学报. 1995,28(3):207~213
    [61]邵凯,徐桂梅,荣威恒,包赛娜,张海鹰,珊丹,于朝晖,王洪荣.不同锌源对绵羊免疫机能的影响[J].动物营养学报. 1996,8(4):51~55
    [62] K.Sahin, M.O.Smith, M.Onderci, N.Sahin. Supplementation of Zinc from or Inorganic Source Improves Performance and Antioxidant Status of Heat-Distressed Quail [J]. Poultry Science. 2005,84:882~887
    [63]成廷水,呙于明.氨基酸锌对产蛋鸡性能及免疫反应的影响[J].饲料研究. 2004,4:1~5
    [64]张日俊,周毓平,黄燕,杨汉春.锌对肉仔鸡免疫器官生长发育及免疫功能调节作用的研究[J].畜牧兽医学报. 1999,30(6):504~512
    [65]刘昌盛,乐国伟,施用晖,虞泽鹏,艾正亚.不同锌源对肉仔鸡的饲养效果[J].动物科学与动物医学. 2002,19(11):51~53
    [66]钟映梅,王力强,孙刚,虞塞明,张君,郭昭林.锌对鹅铜锌超氧化物歧化酶和丙二醛含量影响变化的研究[J].现代畜牧兽医. 2007,3:11~13
    [67] CUI Heng-min, FANG Jing, PENG Xi, DENG Jun-liang. Effect of Zinc on Peripheral Blood T-lymphocyte in the Duckling[J].中国兽医学报, 2004,24 (1):66~68
    [68] Short, F.J., Gorton, P. Wiseman, et al. Determination of titanium dioxide added as an inert marker in chicken digestibility studies[J]. Animal Feed Science Technology. 1996,59:215~221
    [69]吴灵英.肉鸡日粮中不同锌源生物学价值的研究[J].武汉工业学院学报. 2002,1:44~46
    [70]单安山,王安,许振英.饲粮锌和钙水平对产蛋鸡生产性能、血液生化指标和组织中锌含量的影响[J].畜牧兽医学报. 1990,21(4):295~301
    [71]张希兵,刘兵.肉鸡低血糖尖峰死亡综合症的诊治[J].中国家禽. 2005,27(3):28
    [72]王竹.锌和胰岛素糖尿病的关系[J].国外医学卫生学分册. 1999,(6):343~347
    [73] Ming-Der Chen, Shy-Jane Liou, Pi-Yao Lin, Vivian C. Yang, Paul S. Alexander and Wen-Han Lin. Effects of zinc supplementation on the plasma glucose level and insulinactivity in genetically obese (ob/ob) mice[J]. Biological Trace Element Research. 1998,61(3):303~311
    [74] B. Lachili, P. Faure, C. Ribuot, J. Arnaud, M. J. Richard, M. Sve, and A. Favic Zinc deficiency increases the deleterious effect of high fructose diet on feftal development and free radical activity in rats[C]. Trace Elements in Man and Animals 10,2000,536~537
    [75] A.Ibrahim; B.M. MacKinnon; M.D.B. Burt: The influence of sub-lethal levels of zinc on smoltifying Atlantic salmon Salmo salar and on their subsequent susceptibility to infection with Lepeophtheirus salmonis[J]. Contributions to Zoology,2000,69 (1/2)
    [76]吴玉臣,郭爽,阴正兴,唐光武,苏丽娟.氨基酸螯合锌对断奶仔猪免疫功能的影响[J].畜牧与兽医. 2008,40(5):58~59
    [77] Zhang Jing, Zhou Xu, Shen JingLin, Ding HongHao, Zhao Yun, Tang HongYu, Shi XuDong, Fang HengTong, Yan HaiLiang. Journal of Northeast Agricultural University (English Edition) [J]. 2008,15(2):33~36
    [78]王安,单安山,许振英.饲粮钙锌含量对肉仔鸡生长发育血液生化指标和体内锌含量的影响[J].东北农学院学报. 1989,20(2):146~153
    [79]孙术红,兰翠霞,朱文茹,徐淑华,于辉.甲状腺机能亢进患者血清Cu、Zn-SOD含量检测分析[J].临床医学. 2000,20(2):9~10
    [80] Lu C, Chan JY, Walfish PG. Selective effect of zinc compared to other divalent metals on L-triiodothyronine binding to rat c-erbA alpha and beta proteins [J]. Biochem Int. 1990, 21(1):191~198
    [81] G.A.El-Sisy, A.M.A.Abdel-Razek, A.A.Younis, A.M.AAGhallab, M.S.S.Abdou. Effect of dietary zinc or selenium supplementation on some reproductive hormone level in male baladi goats [J]. Global Veterinarian, 2008,2(2):46~50
    [82] Güng?r ak?ay, Bekir Sami UYANIK, Müfide Nuran ak?ay, Salim Ba?ol TEK?N, Leyla YILDIZ, Halil Zeki TONBUL. T3, T4, TSH, Zn and copper metabolism in hyperthyroidism and hypothyroidism [J]. Turk J Med Res. 1994,12 (3):122~126
    [83]赵宏,焦顺昌,杨俊兰,等.三碘甲状腺原氨酸提高肿瘤细胞化疗敏感性的实验研究[J].肿瘤. 2003,23(5):376~379
    [84]张翼,黄国良,何伟民,等.甲状腺刺激性抗体对甲状腺细胞分泌功能的影响[J].西安交通大学学报(医学版). 2003,24(2):174~176

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700