柴胡属植物化学成分和质量控制研究及天目藜芦化学成分研究
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摘要
柴胡属植物化学成分和质量控制研究及天目藜芦化学成分研究本文研究了伞形科柴胡属植物北柴胡(Bupleurum chinense DC.)中的活性成分及有毒植物大叶柴胡(B. longiradiatum)中的毒性成分。从两种柴胡植物中共分离鉴定了34个化合物,其中10个为新化合物,从而阐明了柴胡属植物有效成分和毒性成分物质基础。并进一步进行了中药柴胡的质量控制研究,考察并建立了基于药效成分和毒性成分的相应的定性、定量分析方法和中药化学指纹图谱分析方法。此外,研究了百合科藜芦属植物天目藜芦(Veratrum schindleri Loes. f.)中的化学成分,从中共分离鉴定了36个化合物,其中2个为新化合物,34个为首次从该植物中分得。
In this study, attempts have been made to isolate and define the bioactive and toxic constituents from plants of Bupleurum chinense and B. longiradiatum, to explore chemical components in Bupleurum and clarify the bioactive and toxic material foundation in this genue. From the two plants, 34 compounds in total have been obtained, including 10 new compounds. Furthermore, different kinds of analical methods, including qualitative and quantitative analysis and chemical fingerprint method, have been developed for the comprehensive quality control of Bupleurum, which will ensure the the safety and effective of Chaihu in clinical usage. In addition, another plant, Veratrum schindleri, was also investigated for chemical constituents. From the entire parts of this plant, 36 compounds were isolated and identified including 2 new compounds and 34 compounds isolated from the plant for the first time.
In this study, attempts have been made to isolate and define the bioactive and toxic constituents from plants of Bupleurum chinense and B. longiradiatum, to explore chemical components in Bupleurum and clarify the bioactive and toxic material foundation in this genue. From the two plants, 34 compounds in total have been obtained, including 10 new compounds. Furthermore, different kinds of analical methods, including qualitative and quantitative analysis and chemical fingerprint method, have been developed for the comprehensive quality control of Bupleurum, which will ensure the the safety and effective of Chaihu in clinical usage. In addition, another plant, Veratrum schindleri, was also investigated for chemical constituents. From the entire parts of this plant, 36 compounds were isolated and identified including 2 new compounds and 34 compounds isolated from the plant for the first time.
引文
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[1]刘沁舡,谭利,白焱晶,梁鸿,赵玉英.柴胡属植物皂苷近10年研究概况.中国中药杂志. 2002, 27, 7-11.
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[3] Oleszek, W., Bialy, Z. Chromatographic determination of plant saponins—An update (2002–2005). J. Chromatogr. A 2006, 1112, 78-91.
[4] Liau, B.C., Hsiao, S.S., Lee, M.R., Jong, T.T., Chiang, S.T. Quality control of Chinese medicinal preparations LC/ ESI(+)/MS/MS analyses of saikosaponin-a and -c as markers of Bupleuri redix samples. J. Pharm. Biomed. Anal. 2007, 43, 1174–1178.
[5] Bao, Y.W., Li, C., Shen, H.W., Nan, F.J. Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry. Anal. Chem. 2004, 76, 4208–4216.
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