滋补肝肾以调控NEI促进神经功能重塑的路径研究
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摘要
目的:探讨滋补肝肾中药复健片促进缺血性卒中后神经功能重塑的路径。方法:基于“滋补肝肾以生髓”的中医理论,以MCAO大鼠为实验对象,通过观察复健片调控神经-内分泌-免疫网络(NEI)自稳态和拮抗胶质瘢痕增生的效应,初步探索滋补肝肾中药促进神经功能重塑的路径。分三部分:(一)利用酶联免疫吸附法、荧光分光度法、放射免疫法、免疫组织化学法检测瘦素(Leptin)、5-HT、血管活性肠肽(VIP)、CD8+T细胞、促肾上腺皮质激素(ACTH)、睾丸酮(T)的变化,研究复健片通过调控NEI以抑制缺血性卒中后过度免疫反应的效应;(二)利用酶联免疫吸附法与免疫组织化学法检测基质金属蛋白酶(MMPS),波形蛋白(Vim)与胶质纤维酸性蛋白(GFAP)的变化,研究免疫反应受抑制后胶质瘢痕增生减弱的效应;(三)根据以往工作基础和本课题的结果从理论上探讨滋补肝肾中药减弱胶质瘢痕增生对神经功能重塑的促进效应。结果:复健片可使血清中Leptin的表达下调;缺血脑皮质中5-HT、VIP及CD8+T细胞表达上调;血浆ACTH及血清T上调;缺血脑皮质中MMP-2、MMP-9活性增强;脑梗死后期Vim及GFAP的表达降低。结论:滋补肝肾中药可通过调控NEI自稳态以拮抗胶质瘢痕增生,进而促进缺血性卒中后神经功能重塑。
Objective: To investigate the effects of one particular therapeutic method of Traditional Chinese Medicine (TCM) namely“the nourishing of liver and kidney”on the promotion of neurogenesis through the regulating of NEI after cerebral ischemic stroke. Methods: Based on the TCM theory that“nourishing liver and kidney can produce marrow”, we set up a MCAO rat model and then utilized a TCM herbal medication called Fujian tablet (which is specifically formulated to“nourish liver and kidney”). We observed its’effects on regulating NEI and inhibiting the hyperplasia of glial scar as one pathway for the promotion of neurogenesis. The research includes 3 parts: Part one, six indexes including Leptin, 5-HT, VIP, CD8+T, ACTH, Testosterone were measured in an enzyme linked immunosorbent assay with radio-immunity and immunohistochemical methods used to evaluate the effects of Fujian tablet on inhibiting excessive immunological reactions after cerebral ischemic stroke by the regulation of NEI. Part 2, four indexes including MMP-2, MMP-9, Vim and GFAP were measured with enzyme linked immunosorbent assay and immunohistochemical methods to evaluate the effects of Fujian tablet on inhibiting the hyperplasia of glial scaring. Part 3, based on the results observed in parts one and two, to evaluate the overall capacity for Chinese herbs to promote neurogenesis. Results: Fujian tablet can decrease the expression of Leptin in blood serum, increase the expression of 5-HT, VIP, CD8+T, MMP-2 and MMP-9 in brain tissue, increase the expression of ACTH and TESTO in blood plasma, and decrease the expression of Vim and GFAP in brain tissue. Conclusions: Traditional Chinese Medicine for the nourishing of liver and kidney can significantly improve the symptoms of functional deficit after cerebral ischemic stroke with one of the important mechanisms being the promotion of neurogenesis. Nourishing the liver and kidney is of great importance in regulating the homeostasis of NEI in order to inhibit the hyperplasia of glial scar, and this is closely related to the promotion of neurogenesis.
Objective: To investigate the effects of one particular therapeutic method of Traditional Chinese Medicine (TCM) namely“the nourishing of liver and kidney”on the promotion of neurogenesis through the regulating of NEI after cerebral ischemic stroke. Methods: Based on the TCM theory that“nourishing liver and kidney can produce marrow”, we set up a MCAO rat model and then utilized a TCM herbal medication called Fujian tablet (which is specifically formulated to“nourish liver and kidney”). We observed its’effects on regulating NEI and inhibiting the hyperplasia of glial scar as one pathway for the promotion of neurogenesis. The research includes 3 parts: Part one, six indexes including Leptin, 5-HT, VIP, CD8+T, ACTH, Testosterone were measured in an enzyme linked immunosorbent assay with radio-immunity and immunohistochemical methods used to evaluate the effects of Fujian tablet on inhibiting excessive immunological reactions after cerebral ischemic stroke by the regulation of NEI. Part 2, four indexes including MMP-2, MMP-9, Vim and GFAP were measured with enzyme linked immunosorbent assay and immunohistochemical methods to evaluate the effects of Fujian tablet on inhibiting the hyperplasia of glial scaring. Part 3, based on the results observed in parts one and two, to evaluate the overall capacity for Chinese herbs to promote neurogenesis. Results: Fujian tablet can decrease the expression of Leptin in blood serum, increase the expression of 5-HT, VIP, CD8+T, MMP-2 and MMP-9 in brain tissue, increase the expression of ACTH and TESTO in blood plasma, and decrease the expression of Vim and GFAP in brain tissue. Conclusions: Traditional Chinese Medicine for the nourishing of liver and kidney can significantly improve the symptoms of functional deficit after cerebral ischemic stroke with one of the important mechanisms being the promotion of neurogenesis. Nourishing the liver and kidney is of great importance in regulating the homeostasis of NEI in order to inhibit the hyperplasia of glial scar, and this is closely related to the promotion of neurogenesis.
引文
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