针灸结合骨立冲剂对去卵巢大鼠骨钙代谢影响的研究
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摘要
目的:通过用针灸、骨立冲剂、针灸结合骨立冲剂及西药尼尔雌醇对去卵巢大鼠骨质疏松症治疗后观察、比较,探讨针灸结合骨立冲剂对去卵巢大鼠骨矿化及参与骨钙代谢的激素、局部因子骨钙素(BGP)mRNA与骨形态发生蛋白-2(BMP-2)mRNA表达的影响,为针灸药物结合治疗骨质疏松症提供实验依据,以期为临床治疗骨质疏松症提供更为有效的方法。
方法:将6月龄雌性SD大鼠去除双侧卵巢建立骨质疏松症模型,随机分为模型组、针灸组、骨立冲剂组(简称骨立组)、针灸结合骨立冲剂组(简称针药结合组)及尼尔雌醇组(简称西药组)。针灸组选取腧穴“大杼(双)”、“肾俞(双)”、“脾俞(双)”、“命门”,针刺30分钟后用艾条温和灸上述腧穴各5分钟。每日1次,10次为1个疗程,疗程间隔休息1天,共治疗6个疗程;骨立组用骨立混悬液灌胃,每日1次,疗程同针灸组;针药结合组则在骨立灌胃后再施针灸组方法治疗;西药组以尼尔雌醇混悬液每周灌胃1次,其余5天灌胃生理盐水,每周休息1天后进入下一疗程,治疗时间同针灸组;假手术组和模型组均用生理盐水灌胃,每日1次,疗程同针灸组。治疗期满后,分别检测下列指标:(1)分别用火焰原子吸收法、钼锑抗比色法测定腰椎骨骨钙、骨磷含量;(2)用双能X线吸收法检测大鼠股骨BMD及用三点弯曲实验测试大鼠股骨骨强度;(3)运用扫描电镜观察大鼠股骨松质骨骨小梁结构形态;(4)采用竞争放射免疫法(RIA)测定血清雌二醇(E_2)、甲状旁腺激素(PTH)、降钙素(CT)、骨钙素(BGP)含量;(5)利用RT-PCR技术检测大鼠股骨中BGP与BMP-2mRNA表达水平。
结果:与假手术组比较,模型组大鼠骨钙、磷含量及骨密度极显著下降(P＜0.01),骨强度明显减弱(P＜0.01),骨微结构破坏,子宫萎缩明显(P＜0.01),血清E_2、BGP、CT含量极显著降低(P＜0.01),PTH含量极显著升高(P＜0.01),骨组织BGPmRNA、BMP-2mRNA表达明显减弱(P＜0.01);通过针灸、骨立及针药结合治疗后,与模型组比较,骨钙、磷含量及骨密度、骨力学参数值不同程度升高(P＜0.05或P＜0.01),骨微结构不同程度改善,子宫萎缩得到不同程度控制,血清E_2、BGP、CT含量极显著增加(P＜0.01),PTH含量极显著下降(P＜0.01),股骨BGPmRNA、BMP-2mRNA表达明显增强(P＜0.01);针药结合组各指标值与针灸组、骨立组比较具有显著性差异(P＜0.05或P＜0.01),与西药组比较除子宫重量、血清E_2、PTH、CT及股骨BGPmRNA、BMP-2mRNA具有显著差异(P＜0.05或P＜0.01)外,骨钙、磷、BMD、骨力学参数值均无差异(P＞0.05),且骨小梁结构形态相似。
结论:(1)绝经后骨质疏松症模型复制成功;(2)针灸、骨立和针药结合均能促进去卵巢大鼠骨质有效的矿化,改善骨微结构及增强股骨强度;(3)针灸、骨立和针药结合均能调节去卵巢大鼠参与骨钙代谢的激素E_2、PTH、CT的表达,刺激骨形成,抑制骨吸收,促进骨钙代谢平衡;(4)针灸、骨立和针药结合均能促进去卵巢大鼠股骨组织BGPmRNA、BMP-2mRNA表达,使骨钙代谢趋于平衡;(5)针药结合对去卵巢大鼠骨钙代谢的调节作用明显优于单纯针灸、骨立的作用。
Objective:To investigate the combinative treatment of acupuncture and Gu-li' s affections on ovariectomized rat' s bone mineralization、hormones involved in the bone metabolism and the expression of BGP mRNAand BMP-2 mRNA as well,the research was designed to observe and compare the results of treatments for ovariectomized rats' osteoporosis by using acupuncture and moxibustion treatment、Gu-li、combination of acupuncture and moxibustion and Gu-li、nilestriol in the hope of providing an experimental basis for the combinative treatment of acupuncture and herb and an effective clinical treatment on osteoporosis.
Method:Female SD rats of six months were established to osteoporosis model by removed the ovaries under anesthesia were randomized into model,acupuncture and moxibustion treatment,Gu-li,combination of acupuncture and moxibustion and Gu-li and nilestriol groups,acupuncture and moxibustion treatment group which received only acupuncture and moxibustion therapy and combination of acupuncture and Gu-li group which received acupuncture and moxibustion plus Chinese herb Gu-li powder therapy.In the two groups,bilateral "Dazhu"、"Shenshu"、"Pishu" and "Mingmen" were punctured with acupuncture needle and fumed with moxa stick once daily and 6 treatment periods altogether.Rats of Gu-li group were drenched with Gu-li powder once daily and nilestriol group was drenched with nilestriol once weekly.After trentment,blood samples were collected from femoral artery.(1)Bone calcium and phosphonium of the lumbar vertebra by using flame atomic absorption method and moly-stibine anti-colormetric method respectively.(2)Bone mineral density of the femur of the rats were detected by using dual energy X-ray bone density instrument and bone strength detected by three-spot bending test.(3)Structure-morph of femur cancellous bone trabecula was observed by sCanning electron microscope.(4) Serum E_2、PTH、CT、BGP were assayed by using radioimmunoassay.(5) Expression of BGP and BMP-2 gene were examined with the method of RT-PCR in bone tissue of osteoporosis rats.
Result:After the rats had been removed the ovaries,bone calcium and phosphonium and bone density lost and nature of bone vitodynamics depressed obviously,shapes of bone trabecula were thinner and quantities were decreased,uterus weight depressed obviously,content of serum E_2、CT、BGP degraded and PTH advanced significantly,BGP and BMP-2 gene expression been weaker than sham group(P＜0.01).Metratrophia was controlled after treatment with the method of combination of acupuncture and Gu-li、acupuncture and Gu-li,serum E_2、CT、BGP raised obviously and PTH decreased moderately,BGP gene advanced and displayed positive correlation compared with serum BGP and BMP-2 gene heightened too,bone calcium and phosphonium and BMD and index bone vitodynamics increased at different degree.combination of acupuncture and Gu-li group surpassed significantly acupuncture treatment and Gu-li group from results of statistical analysis of effects on serum E_2、CT、PTH、BGP and BGP and BMP-2 gene and bone calcium and phosphonium、BMD and index bone vitodynamics(P＜0.05 or P＜0.01).
Conclusion:(1)Model of osteoporosis was successfully duplicated. (2)Acupuncture and moxibustion、Gu-li powder、acombination of acupuncture and Gu-li can all improve and normalize bone matrix mineralization、improve bone microarchitecture and reinforce femoral bone strength.(3)Acupuncture and moxibustion,Gu-li powder、combination of acupuncture and Gu-li can all adjust the expression of hormone of E_2、PTH and CT which participated in bone calcium metabolism.(4)Acupuncture and moxibustion、Gu-li powder and combination of acupuncture and Gu-li can all promote the expression of ovariectomized rats' femur of BGPmRNA and BMP-2mRNA.(5) The treatment of acupuncture and moxibustion combined with Gu-li powde is obviously superior to single-method therapy of acupuncture or Gu-li.
方法:将6月龄雌性SD大鼠去除双侧卵巢建立骨质疏松症模型,随机分为模型组、针灸组、骨立冲剂组(简称骨立组)、针灸结合骨立冲剂组(简称针药结合组)及尼尔雌醇组(简称西药组)。针灸组选取腧穴“大杼(双)”、“肾俞(双)”、“脾俞(双)”、“命门”,针刺30分钟后用艾条温和灸上述腧穴各5分钟。每日1次,10次为1个疗程,疗程间隔休息1天,共治疗6个疗程;骨立组用骨立混悬液灌胃,每日1次,疗程同针灸组;针药结合组则在骨立灌胃后再施针灸组方法治疗;西药组以尼尔雌醇混悬液每周灌胃1次,其余5天灌胃生理盐水,每周休息1天后进入下一疗程,治疗时间同针灸组;假手术组和模型组均用生理盐水灌胃,每日1次,疗程同针灸组。治疗期满后,分别检测下列指标:(1)分别用火焰原子吸收法、钼锑抗比色法测定腰椎骨骨钙、骨磷含量;(2)用双能X线吸收法检测大鼠股骨BMD及用三点弯曲实验测试大鼠股骨骨强度;(3)运用扫描电镜观察大鼠股骨松质骨骨小梁结构形态;(4)采用竞争放射免疫法(RIA)测定血清雌二醇(E_2)、甲状旁腺激素(PTH)、降钙素(CT)、骨钙素(BGP)含量;(5)利用RT-PCR技术检测大鼠股骨中BGP与BMP-2mRNA表达水平。
结果:与假手术组比较,模型组大鼠骨钙、磷含量及骨密度极显著下降(P＜0.01),骨强度明显减弱(P＜0.01),骨微结构破坏,子宫萎缩明显(P＜0.01),血清E_2、BGP、CT含量极显著降低(P＜0.01),PTH含量极显著升高(P＜0.01),骨组织BGPmRNA、BMP-2mRNA表达明显减弱(P＜0.01);通过针灸、骨立及针药结合治疗后,与模型组比较,骨钙、磷含量及骨密度、骨力学参数值不同程度升高(P＜0.05或P＜0.01),骨微结构不同程度改善,子宫萎缩得到不同程度控制,血清E_2、BGP、CT含量极显著增加(P＜0.01),PTH含量极显著下降(P＜0.01),股骨BGPmRNA、BMP-2mRNA表达明显增强(P＜0.01);针药结合组各指标值与针灸组、骨立组比较具有显著性差异(P＜0.05或P＜0.01),与西药组比较除子宫重量、血清E_2、PTH、CT及股骨BGPmRNA、BMP-2mRNA具有显著差异(P＜0.05或P＜0.01)外,骨钙、磷、BMD、骨力学参数值均无差异(P＞0.05),且骨小梁结构形态相似。
结论:(1)绝经后骨质疏松症模型复制成功;(2)针灸、骨立和针药结合均能促进去卵巢大鼠骨质有效的矿化,改善骨微结构及增强股骨强度;(3)针灸、骨立和针药结合均能调节去卵巢大鼠参与骨钙代谢的激素E_2、PTH、CT的表达,刺激骨形成,抑制骨吸收,促进骨钙代谢平衡;(4)针灸、骨立和针药结合均能促进去卵巢大鼠股骨组织BGPmRNA、BMP-2mRNA表达,使骨钙代谢趋于平衡;(5)针药结合对去卵巢大鼠骨钙代谢的调节作用明显优于单纯针灸、骨立的作用。
Objective:To investigate the combinative treatment of acupuncture and Gu-li' s affections on ovariectomized rat' s bone mineralization、hormones involved in the bone metabolism and the expression of BGP mRNAand BMP-2 mRNA as well,the research was designed to observe and compare the results of treatments for ovariectomized rats' osteoporosis by using acupuncture and moxibustion treatment、Gu-li、combination of acupuncture and moxibustion and Gu-li、nilestriol in the hope of providing an experimental basis for the combinative treatment of acupuncture and herb and an effective clinical treatment on osteoporosis.
Method:Female SD rats of six months were established to osteoporosis model by removed the ovaries under anesthesia were randomized into model,acupuncture and moxibustion treatment,Gu-li,combination of acupuncture and moxibustion and Gu-li and nilestriol groups,acupuncture and moxibustion treatment group which received only acupuncture and moxibustion therapy and combination of acupuncture and Gu-li group which received acupuncture and moxibustion plus Chinese herb Gu-li powder therapy.In the two groups,bilateral "Dazhu"、"Shenshu"、"Pishu" and "Mingmen" were punctured with acupuncture needle and fumed with moxa stick once daily and 6 treatment periods altogether.Rats of Gu-li group were drenched with Gu-li powder once daily and nilestriol group was drenched with nilestriol once weekly.After trentment,blood samples were collected from femoral artery.(1)Bone calcium and phosphonium of the lumbar vertebra by using flame atomic absorption method and moly-stibine anti-colormetric method respectively.(2)Bone mineral density of the femur of the rats were detected by using dual energy X-ray bone density instrument and bone strength detected by three-spot bending test.(3)Structure-morph of femur cancellous bone trabecula was observed by sCanning electron microscope.(4) Serum E_2、PTH、CT、BGP were assayed by using radioimmunoassay.(5) Expression of BGP and BMP-2 gene were examined with the method of RT-PCR in bone tissue of osteoporosis rats.
Result:After the rats had been removed the ovaries,bone calcium and phosphonium and bone density lost and nature of bone vitodynamics depressed obviously,shapes of bone trabecula were thinner and quantities were decreased,uterus weight depressed obviously,content of serum E_2、CT、BGP degraded and PTH advanced significantly,BGP and BMP-2 gene expression been weaker than sham group(P＜0.01).Metratrophia was controlled after treatment with the method of combination of acupuncture and Gu-li、acupuncture and Gu-li,serum E_2、CT、BGP raised obviously and PTH decreased moderately,BGP gene advanced and displayed positive correlation compared with serum BGP and BMP-2 gene heightened too,bone calcium and phosphonium and BMD and index bone vitodynamics increased at different degree.combination of acupuncture and Gu-li group surpassed significantly acupuncture treatment and Gu-li group from results of statistical analysis of effects on serum E_2、CT、PTH、BGP and BGP and BMP-2 gene and bone calcium and phosphonium、BMD and index bone vitodynamics(P＜0.05 or P＜0.01).
Conclusion:(1)Model of osteoporosis was successfully duplicated. (2)Acupuncture and moxibustion、Gu-li powder、acombination of acupuncture and Gu-li can all improve and normalize bone matrix mineralization、improve bone microarchitecture and reinforce femoral bone strength.(3)Acupuncture and moxibustion,Gu-li powder、combination of acupuncture and Gu-li can all adjust the expression of hormone of E_2、PTH and CT which participated in bone calcium metabolism.(4)Acupuncture and moxibustion、Gu-li powder and combination of acupuncture and Gu-li can all promote the expression of ovariectomized rats' femur of BGPmRNA and BMP-2mRNA.(5) The treatment of acupuncture and moxibustion combined with Gu-li powde is obviously superior to single-method therapy of acupuncture or Gu-li.
引文
[1]李恩,薛延,王洪复,等.骨质疏松症现代诊断与治疗[M].人民卫生出版社,2005:86
[2]薛延.骨质疏松症的流行病学概况[J].新医学,2007,38(1):7-8
[3]张乃钲.中西医结合治疗绝经后骨质疏松症的研究[J].中国中医骨伤科杂志,2000,3(1):14
[4]高明,杨华元,穆敬平.针灸治疗骨质疏松症的现状及思考[J].上海针灸杂志,2007,26(2):40-42
[5]承彬.补肾活血方药治疗绝经后骨质疏松症研究进展[J].吉林中医药,2006,26(9):77-78
[6]蓝肇熙,王杰龙,李凌志.骨立拮抗维甲酸所致大鼠骨质疏松的实验研究[J].华西药学杂志,2003,18(4):250-252
[7]蓝肇熙,马界.骨立对去卵巢大鼠骨质疏松症防治作用的实验研究[J].四川中医,2006,24(11):11-14
[8]蓝肇熙,刘建宇,陈成,等.骨立对去卵巢大鼠骨质疏松症骨生物力学影响的实验研究[J].四川中医,2006,24(10):16-19
[9]陈成.骨疏灵(骨立)对去卵巢大鼠免疫功能影响的实验研究[D].成都体育学院硕士学位论文,2006
[10]李凌志.骨疏灵(骨立)治疗原发性骨质疏松症的临床研究[D].成都体育学院硕士学位论文,2004
[11]马界,胡云光,张达惠.针刺对去卵巢骨质疏松症大鼠骨代谢及雌二醇影响的实验研究[J].针刺研究,2008
[12]邓春雷,殷克敬主编.实验针灸学[M].人民卫生出版社,1998:132
[13]丁晓虹,陈文华.实验针灸中针刺大鼠的心得[J].针刺研究,2006,31(5):318
[14]施新猷.现代医学实验动物学[M].北京:人民军医出版社,2000,334-335
[15]刘庆思,庄洪,黄宏兴.骨质疏松症中西医结合治疗[M].人民卫生出版社,2006
[16]程志安,萧劲夫.从IGFs和GH研究肾虚在OP发病机制中作用的病理生理基础[J].中国中医骨伤科杂志,2000,8(2):43
[17]贾经汉,邱新建,陈志坚,等.常用骨质疏松动物模型特点的综述[J].广西中医学院学报,2006,9(4):76-79
[18]D.D.Thompson,H.A.Simmons,C.M.Pirie,et al.FDA guidelines and animal models for osteoporosis[J].Bone,1995,17(10):25
[19]韦永中,陶松年,杨国平.等.去势对不同月龄雌性大鼠骨丢失的影响[J].南京医科大学学报,1999,19(3):203-205
[20]徐淑云主编.药理学实验方法[M].北京:人民出版社,1991
[21]福田俊,板田治三.去势大鼠骨代谢随时间变化的影响[J].日骨形态志,1991,3(1):89
[22]沈自尹.肾的研究(续集)[M].上海:上海科学技术出版社,2000:295
[23]马斌,老年性骨质疏松症从肾论治的理论基础[J].中国中医骨伤科,3(2):51-53
[24]魏绍斌,杨家林.绝经后骨质疏松症与肾虚的关系[J].中国老年学杂志,2006,16(6):337-338
[25]陈训华,危剑安,陈燕平,等.补肾方药对去卵巢雌鼠骨质疏松症防治作用的研究[J].中国骨伤,2004,11(2):12
[26]王文健,沈自伊,张新民,等.补肾法对老年男性下丘脑—垂体—性腺轴作用的临床和实验研究[J].中医杂志,2006,27(4):35
[27]侯灿,对中医脾本质功能探讨[J].新中医杂志,2007,1(11):10
[28]罗为民,符诗聪,杜宁.健脾补肾法防治绝经后骨质疏松症的量学研究[J].中国中医骨伤科杂志,2005,11(2):7-10
[29]Rodan GA.Perspectives:Mechanical Loading,etrogen deficiency,and the coupling of bone resorption[J].J Bone Miner Res,1999,6(13):527
[30]蒋瑞峰.甲襞微循环障碍与中医血瘀证之关系探讨[J].新中医杂志,2003,8(8):48-49
[31]李沛,陈贵珍,黄彼玉,等.针药结合对绝经后女性生殖内分泌影响的临床研究.福建中医学院学报[J],2003,13(6):21-23
[32]金观源,相嘉嘉,金雷.临床针灸反射学[M].北京:科学技术出版社,2004
[33]谢林,郭振球,姚共和.绝经后骨质疏松中医辨证分析[J].中国医药学报,2003,14(3):35-39
[34]张涛,杭群.针灸现代研究与临床[M].中国医药科技出版社,1998
[35]刘炎编著.中华特效奇穴精粹[M].上海科学技术文献出版社,2005
[36]李广霞.针灸督脉为主治疗老年性骨质疏松症28例临床报道[J].中国针灸,2005,12(9):529
[37]赵英侠,邵水金,余安胜.命门穴区与卵巢、肾上腺的传入神经节段性分布的关系HRP 法的研究[J].针刺研究,2002,24(8):294-296
[38]李僖如,董润生,朱现民,主编.针灸新知识辞典[M].人民卫生出版社,2000
[39]林琳,战雅莲,李红珠.灸疗治百病[M].北京:科学技术文献出版社,2007:13
[40]吴明霞,吴炳煌,刘献祥.艾灸对绝经后骨质疏松症影响的实验和临床研究[J].中国针灸,2002,22(5):334
[41]黄泰康.常用中药成份与药理手册[M].北京:中国医药科技出版社,1999
[42]郑广娟.白术对小白鼠S_(180)肉瘤的抑瘤作用及肿瘤凋亡相关基因bcl—2表达的影响[J].生物医学工程研究,2003,22(3):48-50
[43]马金,郑洪新.补肾中药防治骨质疏松的细胞分子生物学机理探要[J].中医药学刊,2002,20(20):161-162
[44]徐树楠.中药临床应用大全(M).河北科学技术出版社.1999
[45]丁桂枝,刘忠厚,周勇.中西医结合防治骨质疏松症的基础与临床研究进展[J].中国骨质疏松症杂志,1997,3(2):81
[46]Nordin BEC.The calcium contriversy[J].Osteoporos Int.2007,7(3):517
[47]朱太咏,张戈,付汝田,等.仙灵骨葆改善骨质疏松大鼠股骨骨生物力学性能的作用及机制研究[J].中国老年学杂志,2007,27(21):114-118
[48]叶丽卡,陈济志,刘四海,等.淫羊藿在大鼠体内的药代动力学[J].中国药学杂志,2004,34(1):33-36
[49]董进.骨质疏松的现代诊断与治疗[M].北京:中国医药科技出版社,2001
[50]Steiniche T.Bone histomorphometry in the pathophysiological evaluation of primary and secondary osteoporosis and various treatment modalities[J].APMIS,2005,51(2):103
[51]Johnston CC,Slemenda CW.Pathogenesis of osteoporosis[J].Bone,2005,17(2):19
[52]Prince RL,Dick I,Devine A,et al.The effects of menopause and age on calcitropic hormones:A cross-sectional study of 655 healthy women aged 35 to 90[J].J Bone Miner Res,2005,10(6):835-842
[53]杨爱红,董砚虎.骨质疏松与相关激素研究进展[J].青岛医药卫生,2003,35(4):287-289
[54]Arnett JR,Dempster DW.A comparative study of disaggregated chick and rat osteoclasts in vitro: effects of calcitonin and prostaglandins[J].Endocrinology. 2007,120(2):602-608
[55]Nicholson GC, Moseley JM, Sexton PM, et al. abundant calcitonin receptors in isolated rat osteoclasts[J]. J Clin Invest,1986, 78(2):355-360
[56] Fenton AJ, Maxtin TJ, nicholosn GC. Long-Term culture of disaggregated rat osteoclasts: inhibition of bone resorption and reduction of osteoclast-like cell numuber by calcitionin and PTH rp[J]. cell Phtsiol, 2003,15(5):18
[57]Sakai K, Yamada S, Yamada K. Effect of ovariectomy on parafollicular cells in the rat[J]. Okajimas Folia Anat Jpn,2000, 76(5):311-319
[58]Kameda T, Mano H, Yuasa T, et al. Estrogen inhibits bone resorption by directly inducing apoptosis of the bone-resorption osteoclasts[J]. J Exp Med.2007,186(4): 489-495
[59] 史传道,汶医宁.原发性骨质疏松症的病理探析[J],陕西中医学院学报, 2000,23(2):22-23
[60] Heaney RP, Recker RR, Stegman MR, et al. Calcium absorption in women:Relationships to calcium intake, estrogen status, and age[J]. J Bone Miner Res,2005, 4(4):469-475
[61]Raisz LG. Pathogenesis of osteoporosis: concepts, conflicts, and prospects[J].J Clin Invest. 2005, 115(12):3318-3325
[62]Tsai JA, Rong H, Toning O, et al. Interleukin-1 beta upregulates PTHrP-mRNA expression and protein production and decreases TGF-beta in normal human osteoblast-like cells[J]. Calcified Tissue In.. 2000, 66(5):363~369
[63]Parikka V, Lehenkari P, Sassi ML, et al. Estrogen reduces the depth of resorption pits by disturbing the organic bone matrix degradation activity of mature osteoclasts[J]. Endocrinology. 2001,142(12):5371-5378
[64]Spelsberg TC, subramaniam M, Riggs BL, et al. The actions and interactions of sex steroids and growth factors/cytokines on the skeleton[J]..Mol Endocrinoi,2004,13(6):819-828
[65]Shevde NK, Pike JW. Estrogen modulates the recruitment of muelopoietic cell progenitors in rat thrugh a strimal cell-independent mechanism involving apoptosis[J]. Blood, 2006, 87(2):2683-2693
[66]Oursler MJ, Osdoby P, pyfferoen J, et al. Avian osteoclast as estrogen target cells[J]. Proc Natl Acad Sci VSA,2004, 88(3):6613-6617
[67]Huang WH, Zheng MH. Steroid hormones and bone[J].Histol Histopathol,2003,14(11):1257-1268
[68]Mark C, Horowit Z. Cytokines and estrogen in bone: Antiosteoporotic effects[J].Science, 2003,26(10):626-630
[69]Passeri G, Girasole G, Markus T, et al. 17-estradiol regulates IL-6 production and osteoclast development in murine calvaria cell cultures[J]. J Bone Miner Res,2004, 6(12):263-269
[70]Compston JE. Sex steroids and bone[J]. Physiol Rev, 2001,81(1):419-407
[71]Prince RL, Smith M, Dick IM, et al. Prevention of postmenopausal osteoporosis:A comparative study of exercise, calcium supplementation, and hormon-replacement therapy[J]. N Engl J Med, 2001,325(17): 189-195
[72]黄公怡.骨质疏松症骨的组织结构和力学特性[J].中华骨科杂志,2004,24(11):687-690
[73]盛志峰,戴如春,王鹏.去卵巢大鼠骨小梁的纳米力学性能[J].中华医学杂志,2006,86(8):515-519
[74]Abbasi-Jahromi Sh,Matayoshi A,Kimble R,et al.Bone quality factor analysis:a new noninvasive technique for the measurement of bone density and bonestrength[J].J Bone Miner Res,1996,11(5):594-599
[75]Ammann P,Rizzoli R.Bone strength and its determinants[J].Osteoporos Int.,2003,14 Suppl 3:S13-18
[76]Legrand E,Chappard D,Pascaretti C,et al.Trabecular bone microarchitecture,bone mineral density,and vertebral fracture in male osteoporosis[J].J Bone Miner Res,2000,15(1):13-19
[77]Dempster DW.The contribution of trabecular architecture to cancellous bone quality[J].J Bone Miner Res,2000,15(1):20-23
[78]Schaffler MB,Radin EL,Bun DB.Long-term fatigue behavior of compact bone at low strain magnitude and rate[J].Bone,2000,11:321-326
[79]戴克戎.骨质疏松症导致骨力学性能下降的机理[J].中华骨科杂志,2005,17(6):405
[80]Demetropoulos CK,Willis CB,Goldstein SA.Biomechanical characteris of rat trabecular bone[J].Trans Orthop Res Soc,2003,18:585
[81]Frost HM.The pathomechanics of osteoporosis[J].Clic Orthop Rel Res,2005,20(1):198
[82]王闻博,廖二元,邓小戈,等.绝经早期女性性激素、骨密度及骨代谢指标[J].中华内科杂志,2000,39(4):263-264
[83]陈芝喜,刘庆思,魏合伟,等.补骨活血药对去势鼠血清IL-6和激素水平的影响[J].创伤骨科学报,2001(1):45
[84]邵敏,刘庆思,庄洪,等.补肾中药对骨质疏松大鼠性激素影响的实验研究[J].中国骨质疏松杂志,2005,5(4):23
[85]赵英侠.针灸治疗原发性骨质疏松症的现状与展望[J].中医杂志,2006,41(10):630
[86]李凤英,王绵,徐隆绍.甲状旁腺激素与骨质疏松症[J].天津医科大学学报,2000,2(2):246
[87]Agnusdei D,Civitelli R,Camporeale A,et al.Calcitonin and estrogens[J].J Endocrinol Invest,2000,13(8):625-630
[88]詹志伟,孟迅吾.降钙素与原发性骨质疏松症[J].国外医学·内分泌学分册,2006,16(2):67
[89]Filipovic B,Sosic-Jurjevic B,Nestorovic N,et al.The thyroid C cells of ovariectomized rats treated with estradiol.Histochem Cell Biol[J].2003,120(5):409-414
[90]丁艳,杨隽,司书毅,等.Statin类药物促成骨细胞BMP-2表达机理[J].中国生物工程杂志,2005,25(4):18-21
[91]范书,周培清,刘兰英,等.放射免疫分析法对骨质疏松症病因的初步探讨[J].内蒙古民族大学学报(自然科学版),2005,16(4):414
[92]Leung KS,Cheung WH,Zhang C,et al.Low intensity pulsed ultrasound stimulates osteogenic activity of human periosteal cells[J].Clin Orthop Relat Res,2004,418:253-259
[93]Cho TJ,Gerstenfeld LC,Einhorn TA.Differential temporal expression of members of the transforming growth factor beta superfamily during murine fracture healing[J].J Bone Miner Res,2002,17(3):513-520
[94]Hankemeier S,Grassel S,Plenz G,et al.Alteration of fracture stability influences chondrogenesis,osteogenesis and immigration of macrophages[J].J Orthop Res,2001,19(4):531-538
[95]Weaver CM,Peacock M,Martin BR,et al.Immunoassay for quantifying type Ⅰcollagen degradation products in urine evaluated[J].Clin them,2004,40(23):2022-2025
[96]冯坤,刘月桂,张灵菊,等.高转换型骨质疏松模型的生化特点[J].中国骨质疏松杂志,2007,3(2):25-27
[97]Fitzpatrick LA.Estrogen therapy for postmenopausal osteoporosis[J].Arq Bras Endocrinol Metabol.2006,50(4):705-719
[98]Garnero P,Delmas P,Osteoporosis[J].Endocrinol Merab Clinic North Am,2003,26:913
[99]Wronski TJ,Cintron M,Damm LM,Temporal relationship between loss and increased bone turnover in ovariectomized rats[J].Calcif Tissue Int,2006.43:179-183
[100]章明放、黄凤歧,潭郁彬,等.去势大鼠血清骨钙素水平及意义[J].中国骨质疏松杂志,2005,5(3):82
[101]刘石平,伍汉文.雌激素替代治疗防治绝经后骨质疏松症的研究[J].国外医学·内分泌分册,2000,20(6):81-84
[102]Kesser ER.Bone morphogenetic protein-2:the type Ⅰ procollagen-C proteinase[J].Science,2006,29(7):3604
[103]Yamaguchi A,Katagiri T.Ikada T,et al.Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibites myogenic differentiation in vitro[J].Cell Biol,2006,113(3):681-687
[104]Bauduy M,Asbton BA.Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiatian in W-20-17 stromal cells[J].Endocrinology,2004,13(9):1318-1324
[105]Geesink RG,Hoefnagels NH,Bulstra SK.Osteogenic activity of OP-Ⅰ bone morphogenetic protein(BMP-7)in a human fibular defct[J].Bone Joint Surg(Br),2006,81(4):710-718
[106]Urist MR,Hudak RT,Huo YK,et al.Osteoporosis:a bone morphogenetic protein auto-immune disorder[J].Prog Clin Biol Res.2005.18(7):77-96
[107]刘忠厚.骨质疏松学[M].北京:科学出版社,1998
[108]Ghosh-Choudhury N,Windle J,Koop B.et al.Immortalized Murine Osteoblasts Derived from BMP2-T-Antigen Expressing Transgenic Mice[J].Endocrinology,1996,137(1):331-339
[109]张子军,卢世璧,王继芳,等.骨缺损中内源性BMP的分布及其作用[J].中华外科杂志,2006,34(10):596-598
[110]杨丽,张荣华,朱晓峰,等.骨代谢与TGF-B1、BMP-2的关系[J].山东医药,2004,44(15):57-58
[111]闫实,曲迅,韩金祥.BMP-2与肿瘤关系的研究进展[J].医学综述,2006,12(7):385-387
[112] Choslh-Choudhury N, Chosh-Choudhury Celeste A, et al. Bone morphogenetic protein-2 induces cyclin kinase inhibitor p21 and hypophos phorylation of retinoblastoma protein in estradioal-treated MCF-7 human breast cancer cells[J]. Biochim Biophys Acta, 2000, 1497(2):186-196
[113]Anderson GL, Judd HL, Kaunitz AM, et al. Effects of estrogen plus progestin on gynaecologic cancers and associated diagnostic procedures[J]. JAMA, 2003,290(13):1739-1748
[1]董进.骨质疏松的现代诊断与治疗[M].北京:中国医药科技出版社,2001
[2]金世鑫.钙磷代谢疾病[J].中国骨质疏松杂志,2001,7(4):346
[3]Steiniche T.Bone histomorphometry in the pathophysiological evaluation of primary and secondary osteoporosis and various treatment modalities[J].APMIS,2005,51(2):103
[4]Johnston CC,Slemenda CW.Pathogenesis of osteoporosis[J].Bone,2005,17(2):19
[5]胡迎芬.钙与骨质疏松[J].国外医学·卫生学分册,2004,21(3):156
[6]王焱,张志文.钙与人体机能[J].生物学通报,2001,36(6):14
[7]陈忻,穆阳,李惠莲,等.补钙冲剂在大鼠体内吸收利用的研究[[J].黑龙江医药,2007,10(5):292
[8]朱汉民.钙与骨质疏松症[[J].中国骨质疏松杂志,2001,7(3):262
[9]Nordin BEC.The calcium contriversy[J].Osteoporos Int.2007,7(3):5i7
[10]姬巧云.儿童期钙营养与骨矿代谢[[J].国外医学·妇幼保健分册,2002,13(4):172
[11]杨永光.改善微循环对老年骨质疏松症钙治疗作用研究[[J].医学理论与实践,2005,12(5):256
[12]孟迅吾.钙调节激素的改变与原发性骨质疏松症[J].临床内科研成果杂志,2001,8(2).8
[13]梁向华,刘燕儒,梁洪蒙.补钙对晚孕期骨转换生化指标的影响[J].中国骨质疏松杂志,2002,8(3):263
[14]黄建,孙静.钙的生物利用率与骨健康[[J].国外医学·卫生学分册,2003,30(2):109
[15]吕方,卜秀安.影响钙吸收利用的饮食因素[J].职业与健康,2006,12(2):59
[16]陈保华,武小莉.正确补钙[J].微量元素与健康研究,2002:19(1):72
[17]欧阳芝兰,符克军.影响钙吸收利用的药物及饮食因素[J].国外医学·地理分册,1999,20(2):60
[18]Notelovica M.Am[J].Med Sci,2000,313(1):256
[19]齐若梅,廖福龙,王振纲.细胞内钙的调节机制与临床应用[J].中国药理学通报,2002,18(1):16
[20]王振纲,车艳.细胞外钙受体的结构特点以及临床应用的新领域[J].中国药理学通报,2001,17(3):241
[21]林华,韩祖斌,包丽华,等.钙及维生素D_3治疗骨质疏松症349例疗效评价[J].实用老年医学,2000,14(1):33
[22]刘春萍.钙通道拮抗剂对骨代谢的影响[J].国外医学·老年医学分册,1999,20(2):67
[23]沈霖,杜靖远,杨家玉.补肾密骨液防治绝经后妇女代谢性骨质丢失的初步临床研究[J].中国中医骨伤科,2002,2(4):13
[24]张玲娟.淫羊藿对大鼠下丘脑—垂体—肾上腺/胸腺轴抑制模型的影响[J].中医杂志,2006,37(10):620-622
[25]黄纪明,白树民,胡志祥,等.螺旋藻对模拟失重大鼠钙代谢和骨矿盐密度影响的初步研究[J].中国骨质疏松杂志,2002,8(1):51
[26]李洞.钙代谢影响因素研究[J].国外医学·医学地理分册,1998:19(3):101
[1]王际孝,林振幅,于庆元,等.成年人群骨矿含量及中老年肾虚对骨矿影响的研究[J].河北中医,2000,12(3):40-43
[2]蔡新吉,张燕,黄世林.肾虚证患者骨矿物含量改变的初步探讨[J].中国中西医结合杂志,2004,14(3):154-155
[3]张波,刘向前,姚共和.原发性骨质疏松症肾虚髓亏证与骨密度相关性分析[J].中医正骨,2004,16(10):9-10
[4]村上元孝,龟山正邦著,邹元植译。老年病学[M].北京:人民卫生出版社,1981
[5]叶日乔,刘道兵,贾经汉,等.绝经后骨质疏松症患者骨密度及性激素水平与肾虚证的关系[J].中医正骨,2005,17(2):3-5
[6]胡冰,张胜,邱幸凡,等.骨质疏松症中医病因病机探讨[J].湖北中医杂志.2002,24(12):8-9
[7]谢林,姚共和,郭振球.健脾养胃法治疗骨质疏松症初探[J].湖南中医学院学报,2006,16(4):7-9
[8]Cohn SH.Research about Osteoporosis[J].Metabolism,1977,26(2):171
[9]Rodan GA.Perspectives:Mechanical Loading,etrogen deficiency,and the Coupling of bone resorption[J].J Bone Miner Res,1999,6(13):527
[10]魏之玉,张洪,朱振铎,等.196例原发性骨质疏松症辨证析[J].山东中医学院学报,2006,20(1):30-31
[11]罗为民,符诗聪,杜宁.健脾补肾法防治绝经后骨质疏松症的量学研究[J].中国中医骨伤科杂志,2005,11(2):1-3
[12]蒋瑞峰.甲襞微循环障碍与中医血瘀证之关系探讨[J].新中医杂志,2003,8(8):48-49
[13]毛腾敏,马治中,李顺成,等.血瘀病理模型探索-衰老血瘀模型观测[J].北京人学学报(医学版),2001,13(2):1-3
[14]眭承志,周军,刘志坤.绝经后骨质疏松症血瘀病机的客观初步论证[J].中医研究,2005,18(1):30-33
[15]王静,张兰,张家庆,等.雌激素对血浆内皮素水平的影响[J].中华内分泌代谢杂志,2007,1(4):50
[16]谢林,郭振球,姚共和.绝经后骨质疏松中医辨证分析[J].中国医药学报,2003,14(3):35-39
[17]安胜军,李恩,佟晓旭,等.雌激素受体基因多态性与女性绝经后骨质疏松症中医辨证分型关系的研究[J].中国中西医结合杂志,2000,20(12):907-911
[18]艾双春,翁明,廖方正,等.神阙穴贴药对原发性骨质疏松症白细胞介素-6的影响[J].四川中医,2003,21(2):13
[19]吴良辉.原发性骨质疏松症病因病机及针灸治疗[J].中国中医基础医学杂志,2002,8(3):59
[20]刘炎.针灸补肾健脾法治疗骨质疏松症的临床观察[J].针灸临床杂志,2006,12(7):24
[21]刘献祥,吴明霞,吴炳煌,等.针灸对原发性骨质疏松症影响的实验和临床研究[J].中国骨伤,2000,13(9):519
[22]王东岩,蔡红,卓铁军.针刺背俞穴对骨质疏松症腰椎骨密度的影响[J].湖北中医杂志,2001,4(2):6
[23]吴明霞,吴炳煌,钱松涛,等.针灸对骨质疏松患者骨密度作用的临床研究[J].福建中医学院学报,2002,10(4):33-34
[24]坂井友实.针刺治疗退行性骨质疏松症的临床效果(第2报)[J].日本东洋医学杂志,2004,44(5):127
[25]吴明霞,吴炳煌,刘献祥.艾灸对绝经后骨质疏松症影响的实验和临床研究[J].中国针灸,2002,22(5):334
[26]徐正海.逆灸预防绝经后骨质疏松症的临床观察[J].中国中医药信息杂志,2001,8(1):79
[27]张丽.隔药灸调节老年骨密度的研究[J].中医函授通讯,2007,1(1):35
[28]王东岩,王玲玲,欧阳钢,等.红外灸疗仪穴位治疗对原发性骨质疏松症骨密度的影响[J].国医论坛,2003,15(6):23
[29]熊丽芳,肖亚平.耳针治疗中老年妇女骨质疏松症60例临床观察[J].贵阳中医学院学报,2000,22(2):33-34
[30]陈丽仪,郭元琦.温针为主治疗绝经后的临床观察[J].针灸临床杂志,2005,16(8):35
[31]卓铁军,欧阳钢,申志祥,等.针灸结合骨立对绝经后骨质疏松症骨密度的影响[J].针灸临床杂志,2004,16(11):12
[32]袁慧,汪绿萍,李显明.耳压加钙剂治疗绝经后骨质疏松症45例[J].南京中医药大学学报(自然科学版),2004,17(4):24
[33]刘广霞,张道宗.针灸督脉为主治疗老年性骨质疏松症28例临床报道[J].中国针灸,2005,20(9):529-530
[34]Maras I.电针治疗自发性骨质疏松症[J].国外医学·中医中药分册,2006,18(2):51
[35]刘献祥,吴炳煌,吴明霞,等.针灸对原发性骨质疏松症影响的实验研究[J].福建中医学院学报,2004,10(1):21-24
[36]赵英侠.针灸对实验性骨质疏松症的影响[J].中国针灸,2004,19(5):301-303
[37]赵英侠,严振国,余安胜.针灸对卵巢切除大鼠骨代谢的影响[J].上海针灸杂志,2005,18(5):40-41
[38]赵英侠,邵水金,余安胜,等.“命门”穴区与卵巢、肾上腺的传入神经节段性分布的关系—HRP法的研究[J].针刺研究,2002,24(8):294-296
[39]吴明霞,周淋瑛,陈文列,等.针灸对去卵巢大鼠骨密度、强度及超微结构的影响[J].中国临床康复,2004,8(18):3546-3547
[40]李学武,李晓浪,张露芬,等.天灸防治去卵巢大鼠骨质疏松的实验研究[J].中国骨质疏松杂志,2002,4(3):57-61
[41]李晓泓,李学武,张露芬,等.不同时期天灸对去卵巢大鼠骨量、血清骨钙素、骨力学性能的影响[J].中国骨质疏松杂志,2004,4(4):69-72
[42]张露芬,李学武,李晓泓,等.天灸对实验性骨质疏松的影响[J].中国骨质疏松杂志,2001,7(4):297-299
[43]刘献祥,吴明霞,吴炳煌,等.针灸对实验性骨质疏松性骨折愈合影响的研究[J].中国骨伤,2004,14(2):81-82
[44]刘献祥,沈霖,昊明霞,等.针灸对去卵巢大鼠骨组织TGF-B_1基因、VEGF基因表达及凋亡基因Fas的影响[J].中医杂志,2003,44(11):830-832
[45]萨仁,王富舂,池岛乔.针灸治疗实验性骨质疏松症对肿瘤坏死因子基因表达的影响[J].针刺研究,2004,29(2):140-144
[46]姚晓,马淑兰,陈伯英.电针对去卵巢大鼠脑内催乳素释放肽的影响[J].针刺研究,2004,29(2):135-139
[47]武密山,李恩,林富山.抗骨松帖剂对大鼠骨质疏松神经激素运行通路的调节[J].河北医科大学学报,2002,20(1):33-34
[48]欧阳钢,谢碧红.论原发性骨质疏松症从脾胃论治[J].南京中医药大学学报(自然科学版),2003,16(5):269-270
[49]吴成长.骨质疏松症针灸辨治探析[J].现代中西医结合杂志,2004,13(1):77-78
[50]王宝利,权金星,郭善一,等.淫羊藿对去卵巢大鼠骨组织的细胞介素6-基因表达的影响[J].中华妇产科杂志,2003,35(12):724
[51]马中书,王蕊,邱明才,等.四种补骨中药对去卵巢大鼠骨质疏松形态学的作用[J].中华妇产科杂志,2001,34(2):82-85
[52]李青南,廖进民,吴铁,等.淫羊藿防治羟基脉致雄大鼠骨质疏松的定量研究[J].中国中医骨伤科杂志,2006,4(3):121
[53]张玲娟.淫羊藿对大鼠下丘脑—垂体—肾上腺/胸腺轴抑制模型的影响[J].中医杂志,2006,37(10):620-622
[54]刘树人.补骨脂的药理研究进展[J].中医药信息,2002,5(3):15
[55]徐树楠.中药临床应用大全[M].河北科学技术出版社.1999
[56]马晓松.白术对动物胃肠运动作用机制的探讨[J].中华消化杂志,2004,16(5):261
[57]金维哲.维生素E和白术对延缓小鼠肾脏衰老作用的比较[J].中药药理与临床,2005,12(1):31
[58]李树英.山药健脾养胃作用的研究[J].中药药理与临床,2004,6(1):19
[59]李朝阳.蛇床于素对去卵巢大鼠近侧胫骨代谢影响的定量研究[J].药学学报,2006,3(5):327
[60]丁寅.丹参对骨髓培养中破骨细胞生成的影响[J].实用口腔医学杂志,2004,15(1):10
[61]魏美娟,俞瑾.补肾药对雄性激素致无排卵大鼠垂体及卵巢形态学变化观察[J].中国中西医结合杂志,2003,13(9):521-523
[62]张学庆,邹大金.更年期综合症患者白细胞雌激素受体的变化及六味地黄丸的疗效[J].中国中西医结合杂志,2003,13(9):24-26
[63]宋献文,石印玉,沈培芝,等.补肾中药对骨质疏松大鼠骨骼的影响[J].中国骨伤,2007,5(5):13—15
[64]蓝肇熙,马界.骨立对去卵巢大鼠骨质疏松症防治作用的实验研究[J].四川中医,2006,24(11):11-14
[65]蓝肇熙,王杰龙,李凌志.骨立拮抗维甲酸所致大鼠骨质疏松的实验研究[J].华西药学杂志,2003,18(4):250-252
[66]蓝肇熙,刘建宇,陈成,等.骨立对去卵巢大鼠骨质疏松症骨生物力学影响的实验研究[J].四川中医,2006,24(10):16-19
[67]韩旭华,王世民,杨文珍,等.六味地黄丸防治大鼠去势后骨质疏松症的实验研究[J].中药药理与临床,2001,17(5):4-5
[68]罗为民,符诗聪,杜宁,等.健脾补肾法治绝经后骨质疏松症的计量学研究[J].中国中医骨伤科,1995,3(2):1-4
[69]崔洪英,张柏丽,安秀玲.补肾中药对骨质疏松大鼠骨形态的影响[J].天津中医,2005,11(5):226-227
[70]贾东,李东安,李绍华,等.骨疏康冲剂对大鼠实验性骨质疏松的影响[J].中成药,2006,18(2):32-33
[71]姚共和,谢林,郭振球.金刚健骨片对绝经后骨质疏松症骨形成与骨吸收失衡的双向调节作用[J].中国中医骨伤科杂志,2004,7(2):6-9
[72]王长海,张仲海,马静.二仙汤治疗骨质疏松症50例[J].陕西中医,2006,19(5):205
[73]胡光亮,杜靖远,王洪,等.补肾密骨液对骨代谢影响的体外实验研究[J].中国骨伤, 2000,13(2):83-85
[74]邓伟民,沈有高,贺扬淑,等.补肾壮骨中药对去卵巢雌性大鼠股骨上段成骨、破骨细胞的影响[J].广州中医药大学学报,2006,15(4):281-283
[75]张乃钲.补肾中药治疗绝经后骨质疏松症疗效分析[J].中国中医骨伤科杂志,2006,8(2):15-17
[76]陈坤,于世凤.黔岭藿对体外培养的破骨细胞作用的研究[J].中国骨质疏松杂志,2006,2(3):59
[77]詹红生,石印玉,赵咏芳,等.含补肾益精方的切除卵巢大鼠血清对破骨细胞骨吸收的影响[J].中药新药与临床药理,2001,12(5):326-329
[78]吴铁,张志平,李青南,等.仙珍骨宝抗骨质疏松药效学研究[J].中药新药与临床药理,2006,7(4):17-22
[79]支会英.补肾法对去卵巢大鼠骨质疏松的骨组织ER的影响[J].中国中医基础医学杂志,2001,7(4):42-45
[80]沈霖.密骨片对卵巢切除大鼠的内源性白细胞介素-6的影响[J].中医正骨,2000,12(2):3-4
[81]邓良月.国际针灸学教程[M].第1版.北京:华夏出版社,2004
[82]叶丽卡,陈济志,刘四海,等.淫羊藿在大鼠体内的药代动力学[J].中国药学杂志,2004,34(1):33-36
[2]薛延.骨质疏松症的流行病学概况[J].新医学,2007,38(1):7-8
[3]张乃钲.中西医结合治疗绝经后骨质疏松症的研究[J].中国中医骨伤科杂志,2000,3(1):14
[4]高明,杨华元,穆敬平.针灸治疗骨质疏松症的现状及思考[J].上海针灸杂志,2007,26(2):40-42
[5]承彬.补肾活血方药治疗绝经后骨质疏松症研究进展[J].吉林中医药,2006,26(9):77-78
[6]蓝肇熙,王杰龙,李凌志.骨立拮抗维甲酸所致大鼠骨质疏松的实验研究[J].华西药学杂志,2003,18(4):250-252
[7]蓝肇熙,马界.骨立对去卵巢大鼠骨质疏松症防治作用的实验研究[J].四川中医,2006,24(11):11-14
[8]蓝肇熙,刘建宇,陈成,等.骨立对去卵巢大鼠骨质疏松症骨生物力学影响的实验研究[J].四川中医,2006,24(10):16-19
[9]陈成.骨疏灵(骨立)对去卵巢大鼠免疫功能影响的实验研究[D].成都体育学院硕士学位论文,2006
[10]李凌志.骨疏灵(骨立)治疗原发性骨质疏松症的临床研究[D].成都体育学院硕士学位论文,2004
[11]马界,胡云光,张达惠.针刺对去卵巢骨质疏松症大鼠骨代谢及雌二醇影响的实验研究[J].针刺研究,2008
[12]邓春雷,殷克敬主编.实验针灸学[M].人民卫生出版社,1998:132
[13]丁晓虹,陈文华.实验针灸中针刺大鼠的心得[J].针刺研究,2006,31(5):318
[14]施新猷.现代医学实验动物学[M].北京:人民军医出版社,2000,334-335
[15]刘庆思,庄洪,黄宏兴.骨质疏松症中西医结合治疗[M].人民卫生出版社,2006
[16]程志安,萧劲夫.从IGFs和GH研究肾虚在OP发病机制中作用的病理生理基础[J].中国中医骨伤科杂志,2000,8(2):43
[17]贾经汉,邱新建,陈志坚,等.常用骨质疏松动物模型特点的综述[J].广西中医学院学报,2006,9(4):76-79
[18]D.D.Thompson,H.A.Simmons,C.M.Pirie,et al.FDA guidelines and animal models for osteoporosis[J].Bone,1995,17(10):25
[19]韦永中,陶松年,杨国平.等.去势对不同月龄雌性大鼠骨丢失的影响[J].南京医科大学学报,1999,19(3):203-205
[20]徐淑云主编.药理学实验方法[M].北京:人民出版社,1991
[21]福田俊,板田治三.去势大鼠骨代谢随时间变化的影响[J].日骨形态志,1991,3(1):89
[22]沈自尹.肾的研究(续集)[M].上海:上海科学技术出版社,2000:295
[23]马斌,老年性骨质疏松症从肾论治的理论基础[J].中国中医骨伤科,3(2):51-53
[24]魏绍斌,杨家林.绝经后骨质疏松症与肾虚的关系[J].中国老年学杂志,2006,16(6):337-338
[25]陈训华,危剑安,陈燕平,等.补肾方药对去卵巢雌鼠骨质疏松症防治作用的研究[J].中国骨伤,2004,11(2):12
[26]王文健,沈自伊,张新民,等.补肾法对老年男性下丘脑—垂体—性腺轴作用的临床和实验研究[J].中医杂志,2006,27(4):35
[27]侯灿,对中医脾本质功能探讨[J].新中医杂志,2007,1(11):10
[28]罗为民,符诗聪,杜宁.健脾补肾法防治绝经后骨质疏松症的量学研究[J].中国中医骨伤科杂志,2005,11(2):7-10
[29]Rodan GA.Perspectives:Mechanical Loading,etrogen deficiency,and the coupling of bone resorption[J].J Bone Miner Res,1999,6(13):527
[30]蒋瑞峰.甲襞微循环障碍与中医血瘀证之关系探讨[J].新中医杂志,2003,8(8):48-49
[31]李沛,陈贵珍,黄彼玉,等.针药结合对绝经后女性生殖内分泌影响的临床研究.福建中医学院学报[J],2003,13(6):21-23
[32]金观源,相嘉嘉,金雷.临床针灸反射学[M].北京:科学技术出版社,2004
[33]谢林,郭振球,姚共和.绝经后骨质疏松中医辨证分析[J].中国医药学报,2003,14(3):35-39
[34]张涛,杭群.针灸现代研究与临床[M].中国医药科技出版社,1998
[35]刘炎编著.中华特效奇穴精粹[M].上海科学技术文献出版社,2005
[36]李广霞.针灸督脉为主治疗老年性骨质疏松症28例临床报道[J].中国针灸,2005,12(9):529
[37]赵英侠,邵水金,余安胜.命门穴区与卵巢、肾上腺的传入神经节段性分布的关系HRP 法的研究[J].针刺研究,2002,24(8):294-296
[38]李僖如,董润生,朱现民,主编.针灸新知识辞典[M].人民卫生出版社,2000
[39]林琳,战雅莲,李红珠.灸疗治百病[M].北京:科学技术文献出版社,2007:13
[40]吴明霞,吴炳煌,刘献祥.艾灸对绝经后骨质疏松症影响的实验和临床研究[J].中国针灸,2002,22(5):334
[41]黄泰康.常用中药成份与药理手册[M].北京:中国医药科技出版社,1999
[42]郑广娟.白术对小白鼠S_(180)肉瘤的抑瘤作用及肿瘤凋亡相关基因bcl—2表达的影响[J].生物医学工程研究,2003,22(3):48-50
[43]马金,郑洪新.补肾中药防治骨质疏松的细胞分子生物学机理探要[J].中医药学刊,2002,20(20):161-162
[44]徐树楠.中药临床应用大全(M).河北科学技术出版社.1999
[45]丁桂枝,刘忠厚,周勇.中西医结合防治骨质疏松症的基础与临床研究进展[J].中国骨质疏松症杂志,1997,3(2):81
[46]Nordin BEC.The calcium contriversy[J].Osteoporos Int.2007,7(3):517
[47]朱太咏,张戈,付汝田,等.仙灵骨葆改善骨质疏松大鼠股骨骨生物力学性能的作用及机制研究[J].中国老年学杂志,2007,27(21):114-118
[48]叶丽卡,陈济志,刘四海,等.淫羊藿在大鼠体内的药代动力学[J].中国药学杂志,2004,34(1):33-36
[49]董进.骨质疏松的现代诊断与治疗[M].北京:中国医药科技出版社,2001
[50]Steiniche T.Bone histomorphometry in the pathophysiological evaluation of primary and secondary osteoporosis and various treatment modalities[J].APMIS,2005,51(2):103
[51]Johnston CC,Slemenda CW.Pathogenesis of osteoporosis[J].Bone,2005,17(2):19
[52]Prince RL,Dick I,Devine A,et al.The effects of menopause and age on calcitropic hormones:A cross-sectional study of 655 healthy women aged 35 to 90[J].J Bone Miner Res,2005,10(6):835-842
[53]杨爱红,董砚虎.骨质疏松与相关激素研究进展[J].青岛医药卫生,2003,35(4):287-289
[54]Arnett JR,Dempster DW.A comparative study of disaggregated chick and rat osteoclasts in vitro: effects of calcitonin and prostaglandins[J].Endocrinology. 2007,120(2):602-608
[55]Nicholson GC, Moseley JM, Sexton PM, et al. abundant calcitonin receptors in isolated rat osteoclasts[J]. J Clin Invest,1986, 78(2):355-360
[56] Fenton AJ, Maxtin TJ, nicholosn GC. Long-Term culture of disaggregated rat osteoclasts: inhibition of bone resorption and reduction of osteoclast-like cell numuber by calcitionin and PTH rp[J]. cell Phtsiol, 2003,15(5):18
[57]Sakai K, Yamada S, Yamada K. Effect of ovariectomy on parafollicular cells in the rat[J]. Okajimas Folia Anat Jpn,2000, 76(5):311-319
[58]Kameda T, Mano H, Yuasa T, et al. Estrogen inhibits bone resorption by directly inducing apoptosis of the bone-resorption osteoclasts[J]. J Exp Med.2007,186(4): 489-495
[59] 史传道,汶医宁.原发性骨质疏松症的病理探析[J],陕西中医学院学报, 2000,23(2):22-23
[60] Heaney RP, Recker RR, Stegman MR, et al. Calcium absorption in women:Relationships to calcium intake, estrogen status, and age[J]. J Bone Miner Res,2005, 4(4):469-475
[61]Raisz LG. Pathogenesis of osteoporosis: concepts, conflicts, and prospects[J].J Clin Invest. 2005, 115(12):3318-3325
[62]Tsai JA, Rong H, Toning O, et al. Interleukin-1 beta upregulates PTHrP-mRNA expression and protein production and decreases TGF-beta in normal human osteoblast-like cells[J]. Calcified Tissue In.. 2000, 66(5):363~369
[63]Parikka V, Lehenkari P, Sassi ML, et al. Estrogen reduces the depth of resorption pits by disturbing the organic bone matrix degradation activity of mature osteoclasts[J]. Endocrinology. 2001,142(12):5371-5378
[64]Spelsberg TC, subramaniam M, Riggs BL, et al. The actions and interactions of sex steroids and growth factors/cytokines on the skeleton[J]..Mol Endocrinoi,2004,13(6):819-828
[65]Shevde NK, Pike JW. Estrogen modulates the recruitment of muelopoietic cell progenitors in rat thrugh a strimal cell-independent mechanism involving apoptosis[J]. Blood, 2006, 87(2):2683-2693
[66]Oursler MJ, Osdoby P, pyfferoen J, et al. Avian osteoclast as estrogen target cells[J]. Proc Natl Acad Sci VSA,2004, 88(3):6613-6617
[67]Huang WH, Zheng MH. Steroid hormones and bone[J].Histol Histopathol,2003,14(11):1257-1268
[68]Mark C, Horowit Z. Cytokines and estrogen in bone: Antiosteoporotic effects[J].Science, 2003,26(10):626-630
[69]Passeri G, Girasole G, Markus T, et al. 17-estradiol regulates IL-6 production and osteoclast development in murine calvaria cell cultures[J]. J Bone Miner Res,2004, 6(12):263-269
[70]Compston JE. Sex steroids and bone[J]. Physiol Rev, 2001,81(1):419-407
[71]Prince RL, Smith M, Dick IM, et al. Prevention of postmenopausal osteoporosis:A comparative study of exercise, calcium supplementation, and hormon-replacement therapy[J]. N Engl J Med, 2001,325(17): 189-195
[72]黄公怡.骨质疏松症骨的组织结构和力学特性[J].中华骨科杂志,2004,24(11):687-690
[73]盛志峰,戴如春,王鹏.去卵巢大鼠骨小梁的纳米力学性能[J].中华医学杂志,2006,86(8):515-519
[74]Abbasi-Jahromi Sh,Matayoshi A,Kimble R,et al.Bone quality factor analysis:a new noninvasive technique for the measurement of bone density and bonestrength[J].J Bone Miner Res,1996,11(5):594-599
[75]Ammann P,Rizzoli R.Bone strength and its determinants[J].Osteoporos Int.,2003,14 Suppl 3:S13-18
[76]Legrand E,Chappard D,Pascaretti C,et al.Trabecular bone microarchitecture,bone mineral density,and vertebral fracture in male osteoporosis[J].J Bone Miner Res,2000,15(1):13-19
[77]Dempster DW.The contribution of trabecular architecture to cancellous bone quality[J].J Bone Miner Res,2000,15(1):20-23
[78]Schaffler MB,Radin EL,Bun DB.Long-term fatigue behavior of compact bone at low strain magnitude and rate[J].Bone,2000,11:321-326
[79]戴克戎.骨质疏松症导致骨力学性能下降的机理[J].中华骨科杂志,2005,17(6):405
[80]Demetropoulos CK,Willis CB,Goldstein SA.Biomechanical characteris of rat trabecular bone[J].Trans Orthop Res Soc,2003,18:585
[81]Frost HM.The pathomechanics of osteoporosis[J].Clic Orthop Rel Res,2005,20(1):198
[82]王闻博,廖二元,邓小戈,等.绝经早期女性性激素、骨密度及骨代谢指标[J].中华内科杂志,2000,39(4):263-264
[83]陈芝喜,刘庆思,魏合伟,等.补骨活血药对去势鼠血清IL-6和激素水平的影响[J].创伤骨科学报,2001(1):45
[84]邵敏,刘庆思,庄洪,等.补肾中药对骨质疏松大鼠性激素影响的实验研究[J].中国骨质疏松杂志,2005,5(4):23
[85]赵英侠.针灸治疗原发性骨质疏松症的现状与展望[J].中医杂志,2006,41(10):630
[86]李凤英,王绵,徐隆绍.甲状旁腺激素与骨质疏松症[J].天津医科大学学报,2000,2(2):246
[87]Agnusdei D,Civitelli R,Camporeale A,et al.Calcitonin and estrogens[J].J Endocrinol Invest,2000,13(8):625-630
[88]詹志伟,孟迅吾.降钙素与原发性骨质疏松症[J].国外医学·内分泌学分册,2006,16(2):67
[89]Filipovic B,Sosic-Jurjevic B,Nestorovic N,et al.The thyroid C cells of ovariectomized rats treated with estradiol.Histochem Cell Biol[J].2003,120(5):409-414
[90]丁艳,杨隽,司书毅,等.Statin类药物促成骨细胞BMP-2表达机理[J].中国生物工程杂志,2005,25(4):18-21
[91]范书,周培清,刘兰英,等.放射免疫分析法对骨质疏松症病因的初步探讨[J].内蒙古民族大学学报(自然科学版),2005,16(4):414
[92]Leung KS,Cheung WH,Zhang C,et al.Low intensity pulsed ultrasound stimulates osteogenic activity of human periosteal cells[J].Clin Orthop Relat Res,2004,418:253-259
[93]Cho TJ,Gerstenfeld LC,Einhorn TA.Differential temporal expression of members of the transforming growth factor beta superfamily during murine fracture healing[J].J Bone Miner Res,2002,17(3):513-520
[94]Hankemeier S,Grassel S,Plenz G,et al.Alteration of fracture stability influences chondrogenesis,osteogenesis and immigration of macrophages[J].J Orthop Res,2001,19(4):531-538
[95]Weaver CM,Peacock M,Martin BR,et al.Immunoassay for quantifying type Ⅰcollagen degradation products in urine evaluated[J].Clin them,2004,40(23):2022-2025
[96]冯坤,刘月桂,张灵菊,等.高转换型骨质疏松模型的生化特点[J].中国骨质疏松杂志,2007,3(2):25-27
[97]Fitzpatrick LA.Estrogen therapy for postmenopausal osteoporosis[J].Arq Bras Endocrinol Metabol.2006,50(4):705-719
[98]Garnero P,Delmas P,Osteoporosis[J].Endocrinol Merab Clinic North Am,2003,26:913
[99]Wronski TJ,Cintron M,Damm LM,Temporal relationship between loss and increased bone turnover in ovariectomized rats[J].Calcif Tissue Int,2006.43:179-183
[100]章明放、黄凤歧,潭郁彬,等.去势大鼠血清骨钙素水平及意义[J].中国骨质疏松杂志,2005,5(3):82
[101]刘石平,伍汉文.雌激素替代治疗防治绝经后骨质疏松症的研究[J].国外医学·内分泌分册,2000,20(6):81-84
[102]Kesser ER.Bone morphogenetic protein-2:the type Ⅰ procollagen-C proteinase[J].Science,2006,29(7):3604
[103]Yamaguchi A,Katagiri T.Ikada T,et al.Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibites myogenic differentiation in vitro[J].Cell Biol,2006,113(3):681-687
[104]Bauduy M,Asbton BA.Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiatian in W-20-17 stromal cells[J].Endocrinology,2004,13(9):1318-1324
[105]Geesink RG,Hoefnagels NH,Bulstra SK.Osteogenic activity of OP-Ⅰ bone morphogenetic protein(BMP-7)in a human fibular defct[J].Bone Joint Surg(Br),2006,81(4):710-718
[106]Urist MR,Hudak RT,Huo YK,et al.Osteoporosis:a bone morphogenetic protein auto-immune disorder[J].Prog Clin Biol Res.2005.18(7):77-96
[107]刘忠厚.骨质疏松学[M].北京:科学出版社,1998
[108]Ghosh-Choudhury N,Windle J,Koop B.et al.Immortalized Murine Osteoblasts Derived from BMP2-T-Antigen Expressing Transgenic Mice[J].Endocrinology,1996,137(1):331-339
[109]张子军,卢世璧,王继芳,等.骨缺损中内源性BMP的分布及其作用[J].中华外科杂志,2006,34(10):596-598
[110]杨丽,张荣华,朱晓峰,等.骨代谢与TGF-B1、BMP-2的关系[J].山东医药,2004,44(15):57-58
[111]闫实,曲迅,韩金祥.BMP-2与肿瘤关系的研究进展[J].医学综述,2006,12(7):385-387
[112] Choslh-Choudhury N, Chosh-Choudhury Celeste A, et al. Bone morphogenetic protein-2 induces cyclin kinase inhibitor p21 and hypophos phorylation of retinoblastoma protein in estradioal-treated MCF-7 human breast cancer cells[J]. Biochim Biophys Acta, 2000, 1497(2):186-196
[113]Anderson GL, Judd HL, Kaunitz AM, et al. Effects of estrogen plus progestin on gynaecologic cancers and associated diagnostic procedures[J]. JAMA, 2003,290(13):1739-1748
[1]董进.骨质疏松的现代诊断与治疗[M].北京:中国医药科技出版社,2001
[2]金世鑫.钙磷代谢疾病[J].中国骨质疏松杂志,2001,7(4):346
[3]Steiniche T.Bone histomorphometry in the pathophysiological evaluation of primary and secondary osteoporosis and various treatment modalities[J].APMIS,2005,51(2):103
[4]Johnston CC,Slemenda CW.Pathogenesis of osteoporosis[J].Bone,2005,17(2):19
[5]胡迎芬.钙与骨质疏松[J].国外医学·卫生学分册,2004,21(3):156
[6]王焱,张志文.钙与人体机能[J].生物学通报,2001,36(6):14
[7]陈忻,穆阳,李惠莲,等.补钙冲剂在大鼠体内吸收利用的研究[[J].黑龙江医药,2007,10(5):292
[8]朱汉民.钙与骨质疏松症[[J].中国骨质疏松杂志,2001,7(3):262
[9]Nordin BEC.The calcium contriversy[J].Osteoporos Int.2007,7(3):5i7
[10]姬巧云.儿童期钙营养与骨矿代谢[[J].国外医学·妇幼保健分册,2002,13(4):172
[11]杨永光.改善微循环对老年骨质疏松症钙治疗作用研究[[J].医学理论与实践,2005,12(5):256
[12]孟迅吾.钙调节激素的改变与原发性骨质疏松症[J].临床内科研成果杂志,2001,8(2).8
[13]梁向华,刘燕儒,梁洪蒙.补钙对晚孕期骨转换生化指标的影响[J].中国骨质疏松杂志,2002,8(3):263
[14]黄建,孙静.钙的生物利用率与骨健康[[J].国外医学·卫生学分册,2003,30(2):109
[15]吕方,卜秀安.影响钙吸收利用的饮食因素[J].职业与健康,2006,12(2):59
[16]陈保华,武小莉.正确补钙[J].微量元素与健康研究,2002:19(1):72
[17]欧阳芝兰,符克军.影响钙吸收利用的药物及饮食因素[J].国外医学·地理分册,1999,20(2):60
[18]Notelovica M.Am[J].Med Sci,2000,313(1):256
[19]齐若梅,廖福龙,王振纲.细胞内钙的调节机制与临床应用[J].中国药理学通报,2002,18(1):16
[20]王振纲,车艳.细胞外钙受体的结构特点以及临床应用的新领域[J].中国药理学通报,2001,17(3):241
[21]林华,韩祖斌,包丽华,等.钙及维生素D_3治疗骨质疏松症349例疗效评价[J].实用老年医学,2000,14(1):33
[22]刘春萍.钙通道拮抗剂对骨代谢的影响[J].国外医学·老年医学分册,1999,20(2):67
[23]沈霖,杜靖远,杨家玉.补肾密骨液防治绝经后妇女代谢性骨质丢失的初步临床研究[J].中国中医骨伤科,2002,2(4):13
[24]张玲娟.淫羊藿对大鼠下丘脑—垂体—肾上腺/胸腺轴抑制模型的影响[J].中医杂志,2006,37(10):620-622
[25]黄纪明,白树民,胡志祥,等.螺旋藻对模拟失重大鼠钙代谢和骨矿盐密度影响的初步研究[J].中国骨质疏松杂志,2002,8(1):51
[26]李洞.钙代谢影响因素研究[J].国外医学·医学地理分册,1998:19(3):101
[1]王际孝,林振幅,于庆元,等.成年人群骨矿含量及中老年肾虚对骨矿影响的研究[J].河北中医,2000,12(3):40-43
[2]蔡新吉,张燕,黄世林.肾虚证患者骨矿物含量改变的初步探讨[J].中国中西医结合杂志,2004,14(3):154-155
[3]张波,刘向前,姚共和.原发性骨质疏松症肾虚髓亏证与骨密度相关性分析[J].中医正骨,2004,16(10):9-10
[4]村上元孝,龟山正邦著,邹元植译。老年病学[M].北京:人民卫生出版社,1981
[5]叶日乔,刘道兵,贾经汉,等.绝经后骨质疏松症患者骨密度及性激素水平与肾虚证的关系[J].中医正骨,2005,17(2):3-5
[6]胡冰,张胜,邱幸凡,等.骨质疏松症中医病因病机探讨[J].湖北中医杂志.2002,24(12):8-9
[7]谢林,姚共和,郭振球.健脾养胃法治疗骨质疏松症初探[J].湖南中医学院学报,2006,16(4):7-9
[8]Cohn SH.Research about Osteoporosis[J].Metabolism,1977,26(2):171
[9]Rodan GA.Perspectives:Mechanical Loading,etrogen deficiency,and the Coupling of bone resorption[J].J Bone Miner Res,1999,6(13):527
[10]魏之玉,张洪,朱振铎,等.196例原发性骨质疏松症辨证析[J].山东中医学院学报,2006,20(1):30-31
[11]罗为民,符诗聪,杜宁.健脾补肾法防治绝经后骨质疏松症的量学研究[J].中国中医骨伤科杂志,2005,11(2):1-3
[12]蒋瑞峰.甲襞微循环障碍与中医血瘀证之关系探讨[J].新中医杂志,2003,8(8):48-49
[13]毛腾敏,马治中,李顺成,等.血瘀病理模型探索-衰老血瘀模型观测[J].北京人学学报(医学版),2001,13(2):1-3
[14]眭承志,周军,刘志坤.绝经后骨质疏松症血瘀病机的客观初步论证[J].中医研究,2005,18(1):30-33
[15]王静,张兰,张家庆,等.雌激素对血浆内皮素水平的影响[J].中华内分泌代谢杂志,2007,1(4):50
[16]谢林,郭振球,姚共和.绝经后骨质疏松中医辨证分析[J].中国医药学报,2003,14(3):35-39
[17]安胜军,李恩,佟晓旭,等.雌激素受体基因多态性与女性绝经后骨质疏松症中医辨证分型关系的研究[J].中国中西医结合杂志,2000,20(12):907-911
[18]艾双春,翁明,廖方正,等.神阙穴贴药对原发性骨质疏松症白细胞介素-6的影响[J].四川中医,2003,21(2):13
[19]吴良辉.原发性骨质疏松症病因病机及针灸治疗[J].中国中医基础医学杂志,2002,8(3):59
[20]刘炎.针灸补肾健脾法治疗骨质疏松症的临床观察[J].针灸临床杂志,2006,12(7):24
[21]刘献祥,吴明霞,吴炳煌,等.针灸对原发性骨质疏松症影响的实验和临床研究[J].中国骨伤,2000,13(9):519
[22]王东岩,蔡红,卓铁军.针刺背俞穴对骨质疏松症腰椎骨密度的影响[J].湖北中医杂志,2001,4(2):6
[23]吴明霞,吴炳煌,钱松涛,等.针灸对骨质疏松患者骨密度作用的临床研究[J].福建中医学院学报,2002,10(4):33-34
[24]坂井友实.针刺治疗退行性骨质疏松症的临床效果(第2报)[J].日本东洋医学杂志,2004,44(5):127
[25]吴明霞,吴炳煌,刘献祥.艾灸对绝经后骨质疏松症影响的实验和临床研究[J].中国针灸,2002,22(5):334
[26]徐正海.逆灸预防绝经后骨质疏松症的临床观察[J].中国中医药信息杂志,2001,8(1):79
[27]张丽.隔药灸调节老年骨密度的研究[J].中医函授通讯,2007,1(1):35
[28]王东岩,王玲玲,欧阳钢,等.红外灸疗仪穴位治疗对原发性骨质疏松症骨密度的影响[J].国医论坛,2003,15(6):23
[29]熊丽芳,肖亚平.耳针治疗中老年妇女骨质疏松症60例临床观察[J].贵阳中医学院学报,2000,22(2):33-34
[30]陈丽仪,郭元琦.温针为主治疗绝经后的临床观察[J].针灸临床杂志,2005,16(8):35
[31]卓铁军,欧阳钢,申志祥,等.针灸结合骨立对绝经后骨质疏松症骨密度的影响[J].针灸临床杂志,2004,16(11):12
[32]袁慧,汪绿萍,李显明.耳压加钙剂治疗绝经后骨质疏松症45例[J].南京中医药大学学报(自然科学版),2004,17(4):24
[33]刘广霞,张道宗.针灸督脉为主治疗老年性骨质疏松症28例临床报道[J].中国针灸,2005,20(9):529-530
[34]Maras I.电针治疗自发性骨质疏松症[J].国外医学·中医中药分册,2006,18(2):51
[35]刘献祥,吴炳煌,吴明霞,等.针灸对原发性骨质疏松症影响的实验研究[J].福建中医学院学报,2004,10(1):21-24
[36]赵英侠.针灸对实验性骨质疏松症的影响[J].中国针灸,2004,19(5):301-303
[37]赵英侠,严振国,余安胜.针灸对卵巢切除大鼠骨代谢的影响[J].上海针灸杂志,2005,18(5):40-41
[38]赵英侠,邵水金,余安胜,等.“命门”穴区与卵巢、肾上腺的传入神经节段性分布的关系—HRP法的研究[J].针刺研究,2002,24(8):294-296
[39]吴明霞,周淋瑛,陈文列,等.针灸对去卵巢大鼠骨密度、强度及超微结构的影响[J].中国临床康复,2004,8(18):3546-3547
[40]李学武,李晓浪,张露芬,等.天灸防治去卵巢大鼠骨质疏松的实验研究[J].中国骨质疏松杂志,2002,4(3):57-61
[41]李晓泓,李学武,张露芬,等.不同时期天灸对去卵巢大鼠骨量、血清骨钙素、骨力学性能的影响[J].中国骨质疏松杂志,2004,4(4):69-72
[42]张露芬,李学武,李晓泓,等.天灸对实验性骨质疏松的影响[J].中国骨质疏松杂志,2001,7(4):297-299
[43]刘献祥,吴明霞,吴炳煌,等.针灸对实验性骨质疏松性骨折愈合影响的研究[J].中国骨伤,2004,14(2):81-82
[44]刘献祥,沈霖,昊明霞,等.针灸对去卵巢大鼠骨组织TGF-B_1基因、VEGF基因表达及凋亡基因Fas的影响[J].中医杂志,2003,44(11):830-832
[45]萨仁,王富舂,池岛乔.针灸治疗实验性骨质疏松症对肿瘤坏死因子基因表达的影响[J].针刺研究,2004,29(2):140-144
[46]姚晓,马淑兰,陈伯英.电针对去卵巢大鼠脑内催乳素释放肽的影响[J].针刺研究,2004,29(2):135-139
[47]武密山,李恩,林富山.抗骨松帖剂对大鼠骨质疏松神经激素运行通路的调节[J].河北医科大学学报,2002,20(1):33-34
[48]欧阳钢,谢碧红.论原发性骨质疏松症从脾胃论治[J].南京中医药大学学报(自然科学版),2003,16(5):269-270
[49]吴成长.骨质疏松症针灸辨治探析[J].现代中西医结合杂志,2004,13(1):77-78
[50]王宝利,权金星,郭善一,等.淫羊藿对去卵巢大鼠骨组织的细胞介素6-基因表达的影响[J].中华妇产科杂志,2003,35(12):724
[51]马中书,王蕊,邱明才,等.四种补骨中药对去卵巢大鼠骨质疏松形态学的作用[J].中华妇产科杂志,2001,34(2):82-85
[52]李青南,廖进民,吴铁,等.淫羊藿防治羟基脉致雄大鼠骨质疏松的定量研究[J].中国中医骨伤科杂志,2006,4(3):121
[53]张玲娟.淫羊藿对大鼠下丘脑—垂体—肾上腺/胸腺轴抑制模型的影响[J].中医杂志,2006,37(10):620-622
[54]刘树人.补骨脂的药理研究进展[J].中医药信息,2002,5(3):15
[55]徐树楠.中药临床应用大全[M].河北科学技术出版社.1999
[56]马晓松.白术对动物胃肠运动作用机制的探讨[J].中华消化杂志,2004,16(5):261
[57]金维哲.维生素E和白术对延缓小鼠肾脏衰老作用的比较[J].中药药理与临床,2005,12(1):31
[58]李树英.山药健脾养胃作用的研究[J].中药药理与临床,2004,6(1):19
[59]李朝阳.蛇床于素对去卵巢大鼠近侧胫骨代谢影响的定量研究[J].药学学报,2006,3(5):327
[60]丁寅.丹参对骨髓培养中破骨细胞生成的影响[J].实用口腔医学杂志,2004,15(1):10
[61]魏美娟,俞瑾.补肾药对雄性激素致无排卵大鼠垂体及卵巢形态学变化观察[J].中国中西医结合杂志,2003,13(9):521-523
[62]张学庆,邹大金.更年期综合症患者白细胞雌激素受体的变化及六味地黄丸的疗效[J].中国中西医结合杂志,2003,13(9):24-26
[63]宋献文,石印玉,沈培芝,等.补肾中药对骨质疏松大鼠骨骼的影响[J].中国骨伤,2007,5(5):13—15
[64]蓝肇熙,马界.骨立对去卵巢大鼠骨质疏松症防治作用的实验研究[J].四川中医,2006,24(11):11-14
[65]蓝肇熙,王杰龙,李凌志.骨立拮抗维甲酸所致大鼠骨质疏松的实验研究[J].华西药学杂志,2003,18(4):250-252
[66]蓝肇熙,刘建宇,陈成,等.骨立对去卵巢大鼠骨质疏松症骨生物力学影响的实验研究[J].四川中医,2006,24(10):16-19
[67]韩旭华,王世民,杨文珍,等.六味地黄丸防治大鼠去势后骨质疏松症的实验研究[J].中药药理与临床,2001,17(5):4-5
[68]罗为民,符诗聪,杜宁,等.健脾补肾法治绝经后骨质疏松症的计量学研究[J].中国中医骨伤科,1995,3(2):1-4
[69]崔洪英,张柏丽,安秀玲.补肾中药对骨质疏松大鼠骨形态的影响[J].天津中医,2005,11(5):226-227
[70]贾东,李东安,李绍华,等.骨疏康冲剂对大鼠实验性骨质疏松的影响[J].中成药,2006,18(2):32-33
[71]姚共和,谢林,郭振球.金刚健骨片对绝经后骨质疏松症骨形成与骨吸收失衡的双向调节作用[J].中国中医骨伤科杂志,2004,7(2):6-9
[72]王长海,张仲海,马静.二仙汤治疗骨质疏松症50例[J].陕西中医,2006,19(5):205
[73]胡光亮,杜靖远,王洪,等.补肾密骨液对骨代谢影响的体外实验研究[J].中国骨伤, 2000,13(2):83-85
[74]邓伟民,沈有高,贺扬淑,等.补肾壮骨中药对去卵巢雌性大鼠股骨上段成骨、破骨细胞的影响[J].广州中医药大学学报,2006,15(4):281-283
[75]张乃钲.补肾中药治疗绝经后骨质疏松症疗效分析[J].中国中医骨伤科杂志,2006,8(2):15-17
[76]陈坤,于世凤.黔岭藿对体外培养的破骨细胞作用的研究[J].中国骨质疏松杂志,2006,2(3):59
[77]詹红生,石印玉,赵咏芳,等.含补肾益精方的切除卵巢大鼠血清对破骨细胞骨吸收的影响[J].中药新药与临床药理,2001,12(5):326-329
[78]吴铁,张志平,李青南,等.仙珍骨宝抗骨质疏松药效学研究[J].中药新药与临床药理,2006,7(4):17-22
[79]支会英.补肾法对去卵巢大鼠骨质疏松的骨组织ER的影响[J].中国中医基础医学杂志,2001,7(4):42-45
[80]沈霖.密骨片对卵巢切除大鼠的内源性白细胞介素-6的影响[J].中医正骨,2000,12(2):3-4
[81]邓良月.国际针灸学教程[M].第1版.北京:华夏出版社,2004
[82]叶丽卡,陈济志,刘四海,等.淫羊藿在大鼠体内的药代动力学[J].中国药学杂志,2004,34(1):33-36
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