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脱氧雪腐镰刀菌烯醇(DON)污染小麦重力分选研究及设备研制
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摘要
脱氧雪腐镰刀菌烯醇(DON)毒素污染小麦的现象广泛存在于全球各国,其对小麦的污染率以及污染水平居于镰刀菌毒素的首位,在赤霉病大规模爆发时更严重。被DON毒素污染的小麦进入食物链,会对人畜产生急、慢性毒性,细胞毒性等,引起肾中毒、肝中毒、生殖异常以及抑制免疫反应,因此全球各国均建立了完善的限量标准,及时剔除毒素超标的籽粒进入食物链极为重要。被镰刀菌及DON毒素污染的小麦籽粒外观皱缩,比重较正常籽粒小,可借助此差异利用重力分选技术将其从正常小麦中分选出来。本论文利用LA-K实验室用重力分选机考察了不同机械因素对DON毒素污染水平不同的小麦的分选效果;基于重力分选机的基础原理,通过考察DON毒素污染籽粒在分选过程中的分布,对其核心部件振动筛进行了设计;试制了重力分选样机,分析了不同机械因素对分选效果的影响,并进行了分选性能评价;通过分析毒素污染籽粒在筛面上形成稳定的理想分布的过程,阐述了研制的重力分选机分选效果显著提升的原因。主要研究结论如下:
     (1)使用现有的重力分选机可将自然污染DON毒素的小麦中的高DON毒素含量小麦籽粒分选出来,对于不同污染程度的小麦,其分选效果与设备的机械参数密切相关。为了高效、经济地将污染小麦中的高DON毒素含量籽粒分选出来,可对不同批次的DON毒素污染小麦在一系列分选试验的基础上建立分选模型,再通过模型的推导得到理想分选效果的机械参数,使用于后续加工的小麦中的DON毒素含量低于我国限量标准;
     (2)设计了一种在出料边带出料装置的振动筛,上层毒素污染籽粒可高度聚集在筛面轻质端侧。该出料装置为一体式结构,紧密固定于筛面出料边,不易脱落,结构紧凑,拆装方便;
     (3)研制的重力分选机仅进料速率、筛面振动频率及风速对筛面毒素污染籽粒料层的形成和分布有不同程度的影响;筛面振幅、横向倾角和纵向倾角在一定范围内的变化对分选效果无显著的影响;
     (4)研制的重力分选机具有结构简单,实施方便,造价低廉,使用方便的优点,在DON毒素污染小麦分选效果方面,经与LA-K重力分选机对比,毒素污染籽粒被分选出的几率更高;
     (5)研制的重力分选机能实现高效分选DON毒素污染籽粒的原因在于其改变了被分选的DON毒素污染小麦在筛面的扩散运动,形成更有利于毒素污染籽粒高度集中分布的涡旋运动,并形成垂直于出料口的条带状料层,因此具有独特的优势。
Deoxynivalenol (DON), commonly referred to as vomitoxin, is one of the predominanttrichothecenes found as natural contaminants in wheat infected by Fusarium, like F. graminearum and F.culmorum. The worldwide occurrence of DON in wheat has been well documented over the last twodecades. The toxins of DON have been proved to be able to cause a variety of toxic effects in bothexperimental animals and livestock, and on some occasions, toxins produced by DON have also beensuspected to cause toxicity in humans. As a consequence, different maximum levels for DON in wheatand wheat-based food have been proposed in different countries, so it is essential to prevent it fromentering the food chain. Wheat with DON become shrivelled and lower in weight than healthy ones andcan be separated on the basis of specific gravity by the use of gravity separators.
     By applying LA-K gravity separator, the effects of machine parameters, including vibrationfrequency, airflow, lateral slope angle, longitudinal angle and amplitude of screen box on separationresult of DON-contaminated wheat were investigated. Basing on the basic principle of gravityseparation technology, a kind of deck, which was the critical component of machine, was designedaccording to the distribution of DON-contaminated wheat on the deck during processing, and animproved separator was designed with the deck, and the effects of six machine parameters ondistribution of DON-contaminated wheat over the deck in separation process were investigated, and theseparation efficiency was evaluated.
     Conclusions are as follows:
     (1) DON-contaminated wheat could be separated from healty ones by gravity separator. For wheatwith different DON content, the separation efficiency was closely related to machine parameters. Inorder to efficiently and economically select wheat with high content of DON, the optimal values of theparameters were deduced by establishing mathematical model for both the percentage of chosenDON-contaminated wheat and its mass fraction of DON and then by synthetic membership scoreconsidered analysis.
     (2) A deck with a kind of discharge device on the discharge edge was designed, the upperDON-contaminated wheat formed centralized distribution near the light side of the deck. The dischargedevice tightly fixed on the deck was characterized by its one-piece structure, unlikely to drop out,compact-sized and easy disassembly.
     (3) The separation efficiency for DON-contaminated wheat with improved gravity separator wasaffected by feeding rate, oscillation frequency and air velocity, while amplitude, lateral slope angle andlongitudinal angle had no impact on the separation result.
     (4) The designed separator was characterized by simple structure, easy implementation, low cost andeasy to use. Compared with LA-K gravity separator, the mass fraction for DON-contaminated fractionwas higher while the proportion was lower, indicating that the improved technology could highlysorting DON-contaminated part from the contaminated wheat.
     (5) With the improved gravity separator, the pathway for DON-contaminated wheat during the course of separation changed, rather than decentralized but gathered as banding near its discharge gate,efficiently and economically removed from healthy wheat.
引文
1. Abbas, H., Mirocha, C., Pawlosky, R., Pusch, D.,(1985). Effect of cleaning, milling, and baking ondeoxynivalenol in wheat[J]. Applied and environmental microbiology,50(2),482-486.
    2. Alkadri, D., Rubert, J., Prodi, A., Pisi, A., Ma es, J., Soler, C.,(2014). Natural co-occurrence ofmycotoxins in wheat grains from Italy and Syria[J]. Food chemistry,157,111-118.
    3. Atanassov, Z., Nakamura, C., Mori, N., Kaneda, C., Kato, H., Jin, Y.-Z., Yoshizawa, T., Murai, K.,(1994). Mycotoxin production and pathogenicity of Fusarium species and wheat resistance toFusarium head blight[J]. Canadian Journal of Botany,72(2),161-167.
    4. Awad, W.A., Ghareeb, K., B hm, J., Zentek, J.,(2010). Decontamination and detoxificationstrategies for the Fusarium mycotoxin deoxynivalenol in animal feed and the effectiveness ofmicrobial biodegradation[J]. Food Additives and Contaminants,27(4),510-520.
    5. Balascio, C., Misra, M., Johnson, H.,(1987a). Particle movement and separation phenomena for agravity separator. I. Development of a Markov probability model and estimation of modelparameters[J]. Transactions of the ASAE-American Society of Agricultural Engineers,1834-1839.
    6. Balascio, C., Misra, M., Johnson, H.,(1987b). Particle movement and separation phenomena for agravity separator. II. Experimental data and performance of distance-transition Markov models[J].Transactions of the ASAE-American Society of Agricultural Engineers,30(6),1840-1847.
    7. Banotai, C., Greene-McDowelle, D., Azcona-Olivera, J., Pestka, J.,(1999). Effects of intermittentvomitoxin exposure on body weight, immunoglobulin levels and haematuria in the B6C3F1mouse[J]. Food and Chemical Toxicology,37(4),343-350.
    8. Basílico, M.L.Z., Pose, G., Ludemann, V., Pinto, V.E.F., Aríngoli, E.E., Ritieni, A., Basílico, J.C.,(2010). Fungal diversity and natural occurrence of fusaproliferin, beauvericin, deoxynivalenol andnivalenol in wheat cultivated in Santa Fe Province, Argentina[J]. Mycotoxin research,26(2),85-91.
    9. Bensassi, F., Zaied, C., Abid, S., Hajlaoui, M.R., Bacha, H.,(2010). Occurrence of deoxynivalenolin durum wheat in Tunisia[J]. Food Control,21(3),281-285.
    10. Berthiller, F., Dall’asta, C., Corradini, R., Marchelli, R., Sulyok, M., Krska, R., Adam, G.,Schuhmacher, R.,(2009). Occurrence of deoxynivalenol and its3-β-D-glucoside in wheat andmaize[J]. Food Additives and Contaminants,26(4),507-511.
    11. Binder, E., Tan, L., Chin, L., Handl, J., Richard, J.,(2007). Worldwide occurrence of mycotoxins incommodities, feeds and feed ingredients[J]. Animal Feed Science and Technology,137(3),265-282.
    12. Boyacio lu, D., Hettiarachchy, N.,(1995). Changes in some biochemical components of wheatgrain that was infected with Fusarium graminearum[J]. Journal of cereal science,21(1),57-62.
    13. Bracacescu, C., Pirna, I., Sorica, C., Popescu, S., Stan, O.,(2012). Experimental Researches onInfluence of Functional Parameters of Gravity Separator on Quality Indicators of SeparationProcess with Application on Cleaning of Wheat Seeds[C].11th International Scientific Conferenceon Engineering for Rural Development,11,16-21.
    14. Br c cescu, C., Popescu, S., enu, I.,(2011). Experimental researches on the influence offunctional parameters of gravity separator on the quality indicators of separation process withapplication on cleaning of wheat designed to milling[J]. Agronomy Series of ScientificResearch/Lucrari Stiintifice Seria Agronomie,54(2).
    15. Bullerman, L.B., Bianchini, A.,(2007). Stability of mycotoxins during food processing[J].International Journal of Food Microbiology,119(1),140-146.
    16. Cendoya, E., Monge, M.P., Palacios, S.A., Chiacchiera, S.M., Torres, A.M., Farnochi, M.C.,Ramirez, M.L.,(2014). Fumonisin occurrence in naturally contaminated wheat grain harvested inArgentina[J]. Food Control,37,56-61.
    17. Cetin, Y., Bullerman, L.B.,(2005). Cytotoxicity of Fusarium mycotoxins to mammalian cellcultures as determined by the MTT bioassay[J]. Food and Chemical Toxicology,43(5),755-764.
    18. Champeil, A., Dore, T., Fourbet, J.,(2004). Fusarium head blight: epidemiological origin of theeffects of cultural practices on head blight attacks and the production of mycotoxins by Fusariumin wheat grains[J]. Plant Science,166(6),1389-1415.
    19. Che kowski, J., Gromadzka, K., St pień,., Lenc, L., Kostecki, M., Berthiller, F.,(2012).Fusarium species, zearalenone and deoxynivalenol content in preharvest scabby wheat heads fromPoland[J]. World Mycotoxin Journal,5(2),133-141.
    20. Chen, F.S.,(1991). Computer-simulated separation process and design parameters of gravityseparator. International Agricultural Mechanization Conference[C]. Proceedings of a conferenceheld in Beijing, China,16-20October1991.,4.26-30.
    21. Cheng, B., Wan, C., Yang, S., Xu, H., Wei, H., Liu, J., Tian, W., Zeng, M.,(2010). Detoxificationof deoxynivalenol by Bacillus strains[J]. Journal of food safety,30(3),599-614.
    22. Clarke, B.,(1977). Principles and practice of seed cleaning. Paper presented at Short Course onSeed Cleaning and Seed Treatment, Lincoln College, University College of Agriculture,Canterbury, New Zealand, May1977. Principles and practice of seed cleaning[C]. Paper presentedat Short Course on Seed Cleaning and Seed Treatment, Lincoln College, University College ofAgriculture, Canterbury, New Zealand, May1977.,28.
    23. Cui, L., Selvaraj, J.N., Xing, F.G., Zhao, Y.J., Zhou, L., Liu, Y.,(2013). A minor survey ofdeoxynivalenol in Fusarium infected wheat from Yangtze-Huaihe river basin region in China[J].Food Control,30(2),469-473.
    24. D’mello, J., Placinta, C., Macdonald, A.,(1999). Fusarium mycotoxins: a review of globalimplications for animal health, welfare and productivity[J]. Animal Feed Science and Technology,80(3),183-205.
    25. Dall'Asta, C., Dall'Erta, A., Mantovani, P., Massi, A., Galaverna, G.,(2013). Occurrence ofdeoxynivalenol and deoxynivalenol-3-glucoside in durum wheat[J]. World Mycotoxin Journal,6(1),83-91.
    26. Desjardins, A.E., Hohn, T.M., McCORMICK, S.P.,(1993). Trichothecene biosynthesis in Fusariumspecies: chemistry, genetics, and significance[J]. Microbiological reviews,57(3),595-604.
    27. Ehrlich, K.C., Daigle, K.W.,(1987). Protein synthesis inhibition by8-oxo-12,13-epoxytrichothecenes[J]. Biochimica et Biophysica Acta (BBA)-General Subjects,923(2),206-213.
    28. Ennouari, A., Sanchis, V., Marín, S., Rahouti, M., Zinedine, A.,(2013). Occurrence ofdeoxynivalenol in durum wheat from Morocco[J]. Food Control,32(1),115-118.
    29. Garda-Buffon, J., Badiale-Furlong, E.,(2010). Kinetics of deoxynivalenol degradation byAspergillus oryzae and Rhizopus oryzae in submerged fermentation[J]. Journal of the BrazilianChemical Society,21(4),710-714.
    30. Giraud, F., Pasquali, M., Jarroudi, M.E., Vrancken, C., Brochot, C., Cocco, E., Hoffmann, L.,Delfosse, P., Bohn, T.,(2010). Fusarium head blight and associated mycotoxin occurrence onwinter wheat in Luxembourg in2007/2008[J]. Food Additives and Contaminants,27(6),825-835.
    31. Gourdain, E., Piraux, F., Barrier-Guillot, B.,(2011). A model combining agronomic and weatherfactors to predict occurrence of deoxynivalenol in durum wheat kernels[J]. World MycotoxinJournal,4(2),129-139.
    32. Grochowicz, J.,(1980). Machines for cleaning and sorting seeds[M]. Foreign ScientificPublications Department of the National Center for Scientific, Technical and EconomicInformation., Warsaw, Poland.
    33. Hazel, C.M., Patel, S.,(2004). Influence of processing on trichothecene levels[J]. ToxicologyLetters,153(1),51-59.
    34. Hsia, C.C., Wu, Z.Y., Li, Y.S., Zhang, F., Sun, Z.T.,(2004). Nivalenol, a main Fusarium toxin indietary foods from high-risk areas of cancer of esophagus and gastric cardia in China, inducedbenign and malignant tumors in mice[J]. Oncology reports,12(2),449-456.
    35. Hu, Z., Xie, H., Ji, F., Wang, H., Peng, B., Tian, L.,(2007). Design of5XZ-5gravity separator[J].Journal of Northwest A&F University-Natural Science Edition,35(7),193-196,201.
    36. Humans, I.W.G.o.t.E.o.C.R.t., Cancer, I.A.f.R.o.,(1993). Some naturally occurring substances:food items and constituents, heterocyclic aromatic amines and mycotoxins[M]. Lyon, France:International Agency for Research on Cancer.
    37. Hussein, H.S., Brasel, J.M.,(2001). Toxicity, metabolism, and impact of mycotoxins on humansand animals[J]. Toxicology,167(2),101-134.
    38. Huwig, A., Freimund, S., K ppeli, O., Dutler, H.,(2001). Mycotoxin detoxication of animal feedby different adsorbents[J]. Toxicology Letters,122(2),179-188.
    39. Ikunaga, Y., Sato, I., Grond, S., Numaziri, N., Yoshida, S., Yamaya, H., Hiradate, S., Hasegawa, M.,Toshima, H., Koitabashi, M.,(2011). Nocardioides sp. strain WSN05-2, isolated from a wheat field,degrades deoxynivalenol, producing the novel intermediate3-epi-deoxynivalenol[J]. Appliedmicrobiology and biotechnology,89(2),419-427.
    40. Islam, R., Zhou, T., Young, J.C., Goodwin, P.H., Pauls, K.P.,(2012). Aerobic and anaerobicde-epoxydation of mycotoxin deoxynivalenol by bacteria originating from agricultural soil[J].World Journal of Microbiology and Biotechnology,28(1),7-13.
    41. Iverson, F., Armstrong, C., Nera, E., Truelove, J., Fernie, S., Scott, P., Stapley, R., Hayward, S.,Gunner, S.,(1995a). Chronic feeding study of deoxynivalenol in B6C3F1male and female mice[J].Teratogenesis, carcinogenesis, and mutagenesis,15(6),283-306.
    42. Iverson, F., Armstrong, C., Nera, E., Truelove, J., Fernie, S., Scott, P., Stapley, R., Hayward, S.,Gunner, S.,(1995b). Chronic feeding study of deoxynivalenol in B6C3F1male and female mice[J].Teratogenesis Carcinogenesis and Mutagenesis,15(6),283-306.
    43. Jackson, L.S., Bullerman, L.B.,(1999). Effect of processing on Fusarium mycotoxins, Impact ofProcessing on Food Safety[M]. Springer, pp.243-261.
    44. JECFA (2001). Deoxynivalenol[R].(Joint FAO/WHO Expert Committee onFood Additives), Fiftysixth meeting. http://www.inchem.org/documents/jecfa/jecmono/v47je05.htm.
    45. Ji, F., Xu, J., Liu, X., Yin, X., Shi, J.,(2014). Natural occurrence of deoxynivalenol andzearalenone in wheat from Jiangsu province, China[J]. Food chemistry,157,393-397.
    46. Jouany, J.P.,(2007). Methods for preventing, decontaminating and minimizing the toxicity ofmycotoxins in feeds[J]. Animal Feed Science and Technology,137(3),342-362.
    47. Kang, Z.S., Buchenauer, H.,(2000). Cytology and ultrastructure of the infection of wheat spikes byFusarium culmorum[J]. Mycological Research,104,1083-1093.
    48. Khan, M.Q., Keith, B.,(2005). Effects of gravity separation and seed treatment on seed quality inwheat[J]. International Journal of Biology and Biotechnology,2(4):929-935.
    49. Khatibi, P.A., Montanti, J., Nghiem, N.P., Hicks, K.B., Berger, G., Brooks, W.S., Griffey, C.A.,Schmale, D.G.,(2011). Conversion of deoxynivalenol to3-acetyldeoxynivalenol in barley-derivedfuel ethanol co-products with yeast expressing trichothecene3-O-acetyltransferases[J]. Biotechnol.Biofuels,4,26.
    50. Kubena, L.F., Huff, W.E., Harvey, R.B., Phillips, T.D., Rottinghaus, G.E.,(1989). Individual andcombined toxicity of deoxynivalenol and T-2toxin in broiler chicks[J]. Poultry Science,68(5),622-626.
    51. Lambert, L., Hines, F., Eppley, R.,(1995). Lack of initiation and promotion potential ofdeoxynivalenol for skin tumorigenesis in Sencar mice[J]. Food and Chemical Toxicology,33(3),217-222.
    52. Lauren, D., Smith, W.,(2001). Stability of the Fusarium mycotoxins nivalenol, deoxynivalenol andzearalenone in ground maize under typical cooking environments[J]. Food Additives&Contaminants,18(11),1011-1016.
    53. Li, D., Ye, Y., Deng, L., Ma, H., Fan, X., Zhang, Y., Yan, H., Deng, X., Li, Y., Ma, Y.,(2013). Geneexpression profiling analysis of deoxynivalenol-induced inhibition of mouse thymic epithelial cellproliferation[J]. Environ Toxicol Pharmacol,36(2),557-566.
    54. Li, F., Wang, W., Ma, J., Yu, C., Lin, X., Yan, W.,(2012). Natural occurrence of maskeddeoxynivalenol in Chinese wheat and wheat-based products during2008-2011[J]. WorldMycotoxin Journal,5(3),221-230.
    55. Li, F., Yu, C., Shao, B., Wang, W., Yu, H.,(2011a). Natural occurrence of masked deoxynivalenoland multi-mycotoxins in cereals from China harvested in2007and2008[J]. Zhonghua Yu Fang YiXue Za Zhi,45(1),57-63.
    56. Li, X.-Z., Zhu, C., de Lange, C., Zhou, T., He, J., Yu, H., Gong, J., Young, J.,(2011b). Efficacy ofdetoxification of deoxynivalenol-contaminated corn by Bacillus sp. LS100in reducing the adverseeffects of the mycotoxin on swine growth performance[J]. Food Additives&Contaminants: Part A,28(7),894-901.
    57. Liu, Y., Walker, F., Hoeglinger, B., Buchenauer, H.,(2005). Solvolysis procedures for thedetermination of bound residues of the mycotoxin deoxynivalenol in Fusarium species infectedgrain of two winter wheat cultivars preinfected with barley yellow dwarf virus[J]. Journal ofAgricultural and Food Chemistry,53(17),6864-6869.
    58. Lohan, S., Dalal, M., Jakhar, S., Punia, R., Dahiya, O.,(2012). Enhancement in wheat (Triticumaestivum) seed quality using specific gravity separator and its economics[J]. Research on crops,13(3),1124-1129.
    59. Ma, Y.Y., Guo, H.W.,(2008). Mini-review of studies on the carcinogenicity of deoxynivalenol[J].Environmental Toxicology and Pharmacology,25(1),1-9.
    60. Miller, J., Taylor, A., Greenhalgh, R.,(1983). Production of deoxynivalenol and related compoundsin liquid culture by Fusarium graminearum[J]. Canadian journal of microbiology,29(9),1171-1178.
    61. Mishra, S., Ansari, K.M., Dwivedi, P.D., Pandey, H.P., Das, M.,(2013). Occurrence ofdeoxynivalenol in cereals and exposure risk assessment in Indian population[J]. Food Control,30(2),549-555.
    62. Monbaliu, S., Van Poucke, C., Detavernier, C.l., Dumoulin, F., Van De Velde, M., Schoeters, E.,Van Dyck, S., Averkieva, O., Van Peteghem, C., De Saeger, S.,(2009). Occurrence of mycotoxinsin feed as analyzed by a multi-mycotoxin LC-MS/MS method[J]. Journal of Agricultural and FoodChemistry,58(1),66-71.
    63. Morooka, N., Uratsuji, N., Yoshizawa, T., Yamamoto, H.,(1972). Studies on the toxic substances inbarley infected with Fusarium spp[J]. Food Hygiene and Safety Science,13(5),368-375.
    64. Moshatati, A., Gharineh, M.,(2012). Effect of grain weight on germination and seed vigor ofwheat[J]. International Journal of Agricultural and Crop Science,4(8),458-460.
    65. Omurtag, G.Z., Beyoglu, D.,(2003). Occurrence of deoxynivalenol (vomitoxin) in processedcereals and pulses in Turkey[J]. Food Additives and Contaminants,20(4),405-409.
    66. Ouyang, Y.L., Li, S.G., Pestka, J.J.,(1996). Effects of vomitoxin (deoxynivalenol) on transcriptionfactor NF-kappa B/Rel binding activity in murine EL-4thymoma and primary CD4(+) T cells[J].Toxicology and applied pharmacology,140(2),328-336.
    67. Pedrosa, K., Borutova, R.,(2011). Synergistic effects of mycotoxins discussed[J]. Feedstuffs,83(19),1-3.
    68. Pestka, J.J.,(2007). Deoxynivalenol: Toxicity, mechanisms and animal health risks[J]. Animal FeedScience and Technology,137(3-4),283-298.
    69. Pestka, J.J., Islam, Z., Amuzie, C.J.,(2008). Immunochemical assessment of deoxynivalenol tissuedistribution following oral exposure in the mouse[J]. Toxicology Letters,178(2),83-87.
    70. Pestka, J.J., Smolinski, A.T.,(2005). Deoxynivalenol: toxicology and potential effects onhumans[J]. Journal of Toxicology and Environmental Health, Part B,8(1),39-69.
    71. Pleadin, J., Vah i, N., Per i, N., evelj, D., Markov, K., Frece, J.,(2013). Fusarium mycotoxins'occurrence in cereals harvested from Croatian fields[J]. Food Control,32(1),49-54.
    72. Poli enská, I., Tvar ek, L.,(2007). Relationships between deoxynivalenol content, presence ofkernels infected by Fusarium spp. pathogens and visually scabby kernels in Czech wheat in2003–2005[J]. Cereal Research Communications,35(3),1437-1448.
    73. Prelusky, D.B., Gerdes, R.G., Underhill, K.L., Rotter, B.A., Jui, P.Y., Trenholm, H.L.,(1994).Effects of low‐level dietary deoxynivalenol on haematological and clinical parameters of thepig[J]. Natural toxins,2(3),97-104.
    74. Prom, L., Horsley, R., Steffenson, B., Schwarz, P.,(1999). Development of Fusarium head blightand accumulation of deoxynivalenol in barley sampled at different growth stages[J]. Journal of theAmerican Society of Brewing Chemists,57(2),60-63.
    75. Raymond, S.L., Smith, T.K., Swamy, H.,(2005). Effects of feeding a blend of grains naturallycontaminated with Fusarium mycotoxins on feed intake, metabolism, and indices of athleticperformance of exercised horses'[J]. Journal of Animal Science,83(6),1267-1273.
    76. Rizzo, A.F., Atroshi, F., Hirvi, T., Saloniemi, H.,(1992). The hemolytic activity of deoxynivalenoland T-2toxin[J]. Natural toxins,1(2),106-110.
    77. Rocha, O., Ansari, K., Doohan, F.,(2005). Effects of trichothecene mycotoxins on eukaryotic cells:a review[J]. Food Additives and Contaminants,22(4),369-378.
    78. Rodrigues, I., Naehrer, K.,(2012). A three-year survey on the worldwide occurrence of mycotoxinsin feedstuffs and feed[J]. Toxins,4(9),663-675.
    79. Rotter, B.A.,(1996). Invited review: Toxicology of deoxynivalenol (vomitoxin)[J]. Journal ofToxicology and Environmental Health Part A,48(1),1-34.
    80. Savi, G.D., Piacentini, K.C., Tibola, C.S., Scussel, V.M.,(2014). Mycoflora and deoxynivalenol inwhole wheat grains (Triticum aestivum L.) from Southern Brazil[J]. Food Additives&Contaminants: Part B, DOI:10.1080/19393210.2014.898337
    81. Scott, P., Kanhere, S., Lau, P., Dexter, J., Greenhalgh, R.,(1983). Effects of experimental flourmilling and breadbaking on retention of deoxynivalenol(vomitoxin) in hard red spring wheat[J].Cereal Chemistry,60(6),421-426.
    82. Scudamore, K.,(2008). Fate of fusarium mycotoxins in the cereal industry: recent UK studies[J].World Mycotoxin Journal,1(3),315-323.
    83. Shotwell, O., Bennett, G., Stubblefield, R., Shannon, G., Kwolek, W., Plattner, R.,(1984).Deoxynivalenol in hard red winter wheat: relationship between toxin levels and factors that couldbe used in grading[J]. Journal-Association of Official Analytical Chemists,68(5),954-957.
    84. Sifuentes dos Santos, J., Souza, T.M., Ono, E.Y.S., Hashimoto, E.H., Bassoi, M.C., Miranda,M.Z.d., Itano, E.N., Kawamura, O., Hirooka, E.Y.,(2013). Natural occurrence of deoxynivalenol inwheat from Paraná State, Brazil and estimated daily intake by wheat products[J]. Food chemistry,138(1),90-95.
    85. krbi, B., Malachova, A., ivan ev, J., Veprikova, Z., Haj lová, J.,(2011). Fusarium mycotoxinsin wheat samples harvested in Serbia: A preliminary survey[J]. Food Control,22(8),1261-1267.
    86. Sobrova, P., Adam, V., Vasatkova, A., Beklova, M., Zeman, L., Kizek, R.,(2010). Deoxynivalenoland its toxicity[J]. Interdisciplinary toxicology,3(3),94-99.
    87. Speijers, G.J.A., Speijers, M.H.M.,(2004). Combined toxic effects of mycotoxins[J]. ToxicologyLetters,153(1),91-98.
    88. Stankovi, S., Levi, J., Ivanovi, D., Krnjaja, V., Stankovi, G., Tan i, S.,(2012). Fumonisin B1and its co-occurrence with other fusariotoxins in naturally-contaminated wheat grain[J]. FoodControl,23(2),384-388.
    89. Sugita-Konsihi, Y., Tanaka, T., Tabata, S., Nakajima, M., Nouno, M., Nakaie, Y., Chonan, T.,Aoyagi, M., Kibune, N., Mizuno, K.,(2006). Validation of an HPLC analytical method coupled toa multifunctional clean-up column for the determination of deoxynivalenol[J]. Mycopathologia,161(4),239-243.
    90. Sundst l Eriksen, G.,(2003). Metabolism and toxicity of trichothecenes[M].
    91. Sydenham, E.W., Thiel, P.G., Marasas, W.F., Shephard, G.S., Van Schalkwyk, D.J., Koch, K.R.,(1990). Natural occurrence of some Fusarium mycotoxins in corn from low and high esophagealcancer prevalence areas of the Transkei, Southern Africa[J]. Journal of Agricultural and FoodChemistry,38(10),1900-1903.
    92. Thuvander, A., Wikman, C., Gadhasson, I.,(1999). In Vitro Exposure of Human Lymphocytes toTrichothecenes: Individual Variation in Sensitivity and Effects of Combined Exposure onLymphocyte Function[J]. Food and Chemical Toxicology,37(6),639-648.
    93. Tiemann, U., Viergutz, T., Jonas, L., Schneider, F.,(2003). Influence of the mycotoxins α-andβ-zearalenol and deoxynivalenol on the cell cycle of cultured porcine endometrial cells[J].Reproductive Toxicology,17(2),209-218.
    94. Tkachuk, R., Dexter, J., Tipples, K.,(1990). Wheat fractionation on a specific gravity table[J].Journal of cereal science,11(3),213-223.
    95. Tkachuk, R., Dexter, J., Tipples, K., NOWICKI, W.,(1991). Removal by Specific Gravity Table ofTombstone Kernels and Associated Trichothecenes from Wheat Infected with Fusarium HeadBlightl[J]. Cereal Chemistry,68(4),428-443l.
    96. Tran, S., Smith, T.,(2014). Conjugation of deoxynivalenol by Alternaria alternata (54028NRRL),Rhizopus microsporus var. rhizopodiformis (54029NRRL) and Aspergillus oryzae (5509NRRL)[J]. Mycotoxin research,30(1),47-53.
    97. Tutelyan, V.A.,(2004). Deoxynivalenol in cereals in Russia[J]. Toxicology Letters,153(1),173-179.
    98. V lkl, A., Vogler, B., Schollenberger, M., Karlovsky, P.,(2004). Microbial detoxification ofmycotoxin deoxynivalenol[J]. Journal of basic microbiology,44(2),147-156.
    99. Van der Fels-Klerx, H., De Rijk, T., Booij, C., Goedhart, P., Boers, E., Zhao, C., Waalwijk, C., Mol,H., Van der Lee, T.,(2012). Occurrence of Fusarium Head Blight species and Fusarium mycotoxinsin winter wheat in the Netherlands in2009[J]. Food Additives&Contaminants: Part A,29(11),1716-1726.
    100. Vandenbroucke, V., Croubels, S., Verbrugghe, E., Boyen, F., De Backer, P., Ducatelle, R., Rychlik,I., Haesebrouck, F., Pasmans, F.,(2009). The mycotoxin deoxynivalenol promotes uptake ofSalmonella Typhimurium in porcine macrophages, associated with ERK1/2induced cytoskeletonreorganization[J]. Veterinary research,40(6),1.
    101. Vesely, D., Vesela, D.,(1995). Embryotoxic effects of a combination of zearalenone and vomitoxin(4-dioxynivalenole) on the chick embryo[J]. Veterinarni medicina,40(9),279-281.
    102. Vesonder, R., Ciegler, A., Jensen, A.,(1973). Isolation of the emetic principle fromFusarium-infected corn[J]. Applied microbiology,26(6),1008-1010.
    103. Wolf, C.E., Bullerman, L.B.,(1998). Heat and pH alter the concentration of deoxynivalenol in anaqueous enviroment[J]. Journal of Food Protection,61(3),365-367.
    104. Wong, L., Abramson, D., Tekauz, A., Leisle, D., McKenzie, R.,(1995). Pathogenicity andmycotoxin production of Fusarium species causing head blight in wheat cultivars varying inresistance[J]. Canadian journal of plant science,75(1),261-267.
    105. Wu, S., Sokhansanj, S., Fang, R., Opoku, A.,(1999). Influence of physical properties and operatingconditions on particle segregation on gravity table[J]. Applied engineering in agriculture,18(3),51-62.
    106. Xu, N.Z., Chen, H.Q., Lu, R.F.,(1987). Testing and research on the main parameters of gravityseed separators[J]. Transactions of the Chinese Society of Agricultural Machinery,18(3),51-62.
    107. Yoshizawa, T., Morooka, N.,(1973). Deoxynivalenol and its monoacetate: new mycotoxins fromFusarium roseum and moldy barley[J]. Agricultural and biological chemistry,37(12),2933-2934.
    108. Young, J.C., Zhu, H., Zhou, T.,(2006). Degradation of trichothecene mycotoxins by aqueousozone[J]. Food and Chemical Toxicology,44(3),417-424.
    109. Yu, H., Zhou, T., Gong, J., Young, C., Su, X., Li, X.-Z., Zhu, H., Tsao, R., Yang, R.,(2010).Isolation of deoxynivalenol-transforming bacteria from the chicken intestines using the approachof PCR-DGGE guided microbial selection[J]. BMC microbiology,10(1),182.
    110. Zhang, X., Xie, T., Li, S., Yan, X., Wang, J., Tan, S., Wang, Z., Fu, C., Wang, F.,(1995). Preventivedetection of fungi and mycotoxins in corn from high risk area of esophageal cancer in Cixiancounty[J]. Chinese Journal of Cancer Research,7(3),172-176.
    111. Zhou, H.-R., Harkema, J.R., Hotchkiss, J.A., Yan, D., Roth, R.A., Pestka, J.J.,(2000).Lipopolysaccharide and the trichothecene vomitoxin (deoxynivalenol) synergistically induceapoptosis in murine lymphoid organs[J]. Toxicological Sciences,53(2),253-263.
    112. Zhou, H.-R., Yan, D., Pestka, J.J.,(1997). Differential cytokine mRNA expression in mice afteroral exposure to the trichothecene vomitoxin (deoxynivalenol): dose response and time course[J].Toxicology and applied pharmacology,144(2),294-305.
    113. Zou, Z.-Y., He, Z.-F., Li, H.-J., Han, P.-F., Meng, X., Zhang, Y., Zhou, F., Ouyang, K.-P., Chen,X.-Y., Tang, J.,(2012). In vitro removal of deoxynivalenol and T-2toxin by lactic acid bacteria[J].Food Science and Biotechnology,21(6),1677-1683.
    114.陈飞,(2012).加工工艺去除小麦中脱氧雪腐镰刀菌烯醇(DON)的研究[D].中国农业科学院.
    115.陈海庆,许乃章,卢仁富,(1986).重力式精选机的研究—分选性能试验及分析[J].浙江大学学报(农业与生命科学版),2,5.
    116.陈龙明,李群伟,侯海峰,(2009). DON与NOC对胃组织致癌作用的实验研究[J].中华肿瘤防治杂志,16(018),1372-1374.
    117.陈侍良,(1985).重力式种子精选机工作原理分析[J].粮油加工与食品机械,11,3.
    118.程亮,伍松陵,沈晗,孙长坡,(2013).脱氧雪腐镰刀菌烯醇降解菌的筛选与鉴定[J].粮油食品科技,21(5),95-97.
    119.崔贵金,(2013).赤霉病麦粒光电分选技术研究[D].河南工业大学.
    120.戴四基,(2013).沿淮及淮北2012年小麦赤霉病流行原因分析与治理对策[J].安徽农业科学,40(36),17557-17558.
    121.丁卫新,(2013).赤霉病对小麦品质影响的研究[J].现代面粉工业,27(4),28-31.
    122.樊平声,沙国栋,沈培银,冯伟民,卢昱宇,(2010).脱氧雪腐镰刀菌烯醇毒性和检测方法研究进展[J].检验检疫科学,(1),39-41.
    123.冯培生,沈建忠,吴海霞,李林霞,戴燕玉,刘凯丽,王莹,张素霞,(2012).体外大鼠肠道菌群代谢脱氧雪腐镰刀菌烯醇的研究[J].分析化学,40(1),119-123.
    124.何成华,(2007).脱氧雪腐镰刀菌烯醇毒性和生物转化的研究[D].南京农业大学.
    125.何敏,(2011).猪肠道微生物对脱氧雪腐镰刀菌烯醇减毒作用菌株的分离鉴定[D].华南理工大学.
    126.侯海峰,陈龙明,韩纪举,李金平,丁国永,邱静,李群伟,(2010).大骨节病病区粮食中脱氧雪腐镰刀菌烯醇和硒元素检测报告[J].中华预防医学杂志,44(7),663-664.
    127.胡志超,计福来,王海鸥,田立佳,彭宝良,钟挺,(2010).高效重力式精选设备的研制[J].江苏农业科学,(2),394-396.
    128.胡志超,谢焕雄,计福来,王海鸥,彭宝良,田立佳,(2007).5XZ-5型重力式精选机的研制[J].西北农林科技大学学报:自然科学版,35(7),490-491.
    129.康振生,黄丽丽,韩青梅,蒋选利,(2004).禾谷镰刀菌在小麦穗部侵染过程的细胞学研究[J].植物病理学报,34(4),329-335.
    130.孔德远,(2002).浅析比重式分选机工作原理及使用技术[J].种子,4,21.
    131.李斌,(1999).镰刀菌毒素DON, NIV的细胞毒性和致突变,致畸,致癌研究进展[J].癌变.畸变.突变,11(4),206-207.
    132.李大伟,陈志申,刘庆元,喻璋,曹志中,窦新平,(1986).小麦赤霉病对产量的损失测定[J].河南农业大学学报,3,360-362.
    133.李革,赵匀,(2001).倾斜气流清选装置中物料的动力学特性,轨迹和分离研究[J].农业工程学报,17(6),22-25.
    134.李海军,孙苏阳,王永军,徐文静,蒋守华,(2008).小麦赤霉病发生原因与防治措施[J].农技服务,25(9),78,87.
    135.李群伟,侯海峰,李亚鲁,韩纪举,王欣农,李晓梅,(2007).脱氧雪腐镰刀菌烯醇致家兔多脏器损伤的实验观察[J].中国地方病防治杂志,22(1),19-22.
    136.李树高,(2008).浅谈麦间清理工艺改造经验[J].面粉通讯,2,6.
    137.李毅念,(2005).萌动小麦重力分选效果的研究[D].南京农业大学.
    138.李毅念,丁为民,卢大新,胡志超,张礼刚,谢焕雄,(2005).小麦精选加工效果的探讨[J].西北农业学报,14(3),49-54.
    139.李毅念,卢大新,丁为民,胡志超,谢焕雄,张礼刚,(2006a).萌动小麦重力分选的试验[J].农业机械学报,36(9),48-52.
    140.李毅念,卢大新,丁为民,胡志超,谢焕雄,张礼刚,(2006b).萌动小麦重力分选效果的试验[J].农业机械学报,37(7),78-82.
    141.李毅念,王俊,(2010).厚度和重力分选稻谷籽粒的品质特性分析[J].农业工程学报,(8),315-319.
    142.李月红,张祥宏,王俊灵,严霞,黄向华,杨建柱,刘艳丽,王凤荣,(2002).脱氧雪腐镰刀菌烯醇对小鼠胸腺细胞凋亡和增殖的影响[J].中国病理生理杂志,18(7),778-781.
    143.李月红,张祥宏,邢凌霄,左连富,严霞,王俊灵,王凤荣,(2005).脱氧雪腐镰刀菌烯醇对人外周血单个核细胞TAP21表达的抑制作用[J].细胞与分子免疫学杂志,21(2).246-248.
    144.梁杰,魏国华,(1998).重力分选机网面上物料运动特性的理论分析[J].农机化研究,(2),38-41.
    145.林林,孙树秋,(2005).脱氧雪腐镰刀菌烯醇致Vero细胞核基因组DNA损伤与修复的彗星实验观察[J].中国地方病防治杂志,19(3),139-141.
    146.刘海生,(2008).种子清选加工原理与主要设备工作原理[J].现代农业科技,(15),259-259.
    147.刘鹏郎,尹龙超,姜衍礼,许本文,(1983).5XZ-1.0型重力式种子精选机筛体的振动分析[J].北京农业机械化学院学报,1,3.
    148.刘孟哲,(2011).凝结芽孢杆菌及芽孢杆菌细胞壁吸附玉米赤霉烯酮毒素及脱氧雪腐镰刀菌烯醇毒素之研究[D].
    149.卢大新,(2001).比重分选机对收获期受雨害小麦的精选分级效果[J].西北农业学报,10(3),87-89.
    150.陆刚,高永清,秦树阳,(1998).小麦中致吐毒素的去毒研究[J].卫生研究, S1,77.
    151.马继光,(2001).国外重力式清选机的发展方向[J].世界农业,7,32-33.
    152.马忠乾,许峰,(1999). OLIVER重力式分选机选型依据[J].现代化农业,(1),35-36.
    153.彭双清,杨进生,(2005).镰刀菌毒素脱氧雪腐镰刀菌烯醇对心肌细胞Ca2+通道的阻滞作用[J].中国预防医学杂志,5(4),241-243.
    154.沈军,牛再兴,(2010).比重分级去石机的调试方法[J].现代面粉工业,1,14.
    155.石海岗,周华英,(2006).食用赤霉病麦中毒原因调查[J].现代预防医学,32(9),1165-1165.
    156.苏迎晨,黄兴国,刘国定,杨开明,(1992).重力分选机主要参数与分选质量的试验研究[J].粮油加工与食品机械,1,35.
    157.田鸿,(2008).往复式比重清选机的应用[J].河北农机,(3),26-26.
    158.汪裕安,吕秋瑾,(1983).重力式清选机分选规律的初步探索[J].农业机械学报,3,57-69.
    159.王会艳,张祥宏,(2000).常见镰刀菌素与细胞凋亡的研究进展[J].卫生研究,29(3),181-183.
    160.王加生,徐达道,(1986).赤霉病麦中毒研究Ⅳ.赤霉病麦粗毒素的致畸与致突变研究[J].真菌学报,5(1),52-62.
    161.王晓云,于雅琴,俞琼,(2007).2005年中国居民膳食DON污染调查及暴露评估[J].长治医学院学报,21(2),101-103.
    162.王艳丰,梁中华,刘兆丰,牛文祥,尹思万,(2005).5XFZ-30.0型重力复式清选机单向倾斜比重筛参数的选择与试验[J].农业工程学报,20(6),115-119.
    163.魏永立,曲长渝,(1996).正压式重力分选机分选质量的因素分析[J].现代化农业,6,35-36.
    164.温纪平,林江涛,温钦豪,姚显伟,(2010).小麦清洁处理技术探讨[J].粮食与饲料工业,(2),10-11.
    165.吴守一,方如明,(1988).种子在重力式精选机台面上的运动规律[J].农业机械学报,19(1),23-30.
    166.吴志远,张帆,夏求洁,(1997).食管癌高发区镰刀菌毒素的动物致癌性[J].中华肿瘤杂志,19(2),156-156.
    167.谢茂昌,王明祖,(1999).小麦赤霉病发病程度与DON含量的关系[J].植物病理学报,29(1),41-44.
    168.谢茂昌,王明祖,(2000).用化学方法脱除赤霉病麦毒素(DON)[J].上海农业学报,16(1),58-61.
    169.徐剑宏,祭芳,王宏杰,王建伟,林凡云,史建荣,(2010).脱氧雪腐镰刀菌烯醇降解菌的分离和鉴定[J].中国农业科学,43(22),4635-4641.
    170.许乃章,陈海庆,卢仁富,(1986).重力式精选机的研究—参数的理论分析[J].浙江大学学报(农业与生命科学版),3,004.
    171.许乃章,陈海庆,卢仁富,(1987).重力式精选机主要参数的研究[J].农业机械学报,3,6.
    172.薛伟龙,陆仕华,(1991). DON在小麦赤霉病菌早期侵染中的作用[J].上海农业学报(A00),30-34.
    173.薛志成,(2009).比重式种子清选机的正确使用[J].种子世界,(11),53-54.
    174.杨敦科,刘兴昌,(1991).关中灌区小麦赤霉病发生程度与产量损失的关系[J].陕西农业科学,(6),30-31.
    175.杨继芝,王继师,龚国淑,陈华保,王学贵,张敏,唐太飞,(2010).不同禾谷镰刀菌对小麦产量及其主要性状的影响[J].河南农业科学,(9),91-95.
    176.应卫东,刘文波,(1998).正压式重力分选机筛体结构及参数对分选质量的影响分析[J].现代化农业,5,32-33
    177.于德水,葛志勇,(1990).小麦的分级清理[J].粮食与饲料工业,3,18-20.
    178.张帆,吴志远,吴健丽,夏求洁,(2000).食管癌高发区粮食中镰刀菌毒素的含量及其致突变作用[J].中华预防医学杂志,34(1),53-53.
    179.张黎骅,张文,吕珍珍,王之盛,(2011).响应面法优化酒糟微波间歇干燥工艺[J].农业工程学报,27(3),369-374.
    180.张艺兵,鲍蕾,褚庆华,主编(2006).农产品中真菌毒素的检测分析[M].化学工业出版社.
    181.赵如芬,张元生,李延云,徐林江,(1986).重力式清选机运动状态分析及工作参数的选择[J].农业工程学报,2(4),81-86.
    182.赵振华,赵文启,(1986).小麦赤霉病病穗空间分布及产量损失率的测定[J].植物保护,5,6.
    183.中国农业机械化科学研究院,(2007).农业机械设计手册[M].中国农业科学技术出版社.
    184.邹程焓,(2011).肉与肉制品中脱氧雪腐镰刀菌烯醇的检测及降解研究[D].西南大学.

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