施氏假单胞菌SC221-M的反硝化特性及其调节草鱼养殖水体水质的研究
|
摘要
为了降低草鱼养殖水体中的氨氮及亚硝酸盐等污染物,通过定向富集的方法筛选到一株具有良好脱氮性能的施氏假单胞菌(Pseudomonas stutzeri) SC221。用钻60对该菌进行辐射诱变,发现该菌的反硝化脱氮能力从50mg/L提高到了100mg/L。经过辐射诱变后的菌株被命名为SC221-M。随后研究了不同环境条件(不同氮源、碳源、碳氮比和温度)对SC221-M生长、脱氮率及反硝化酶基因表达的影响,结果表明SC221-M的最佳脱氮条件为：氮源为亚硝酸钠、碳源为柠檬酸钠,碳氮比为4-8,温度为20-35℃。环境因素能够显著影响SC221-M的生长及其脱氮率,且脱氮率与反硝化酶基因的表达量之间有一定的相关性。
通过Illumina Hiseq2000平台测序和序列组装,发现施氏假单胞菌SC221-M的基因组大小为4545027bp,GC含量为64.01%,共131个scaffold,399个contig。与参考菌株DSM4166的基因组进行比对,发现SC221-M基因组共有60641个SNP,基因区内的同义突变为43396个,非同义突变为10743个。InDel分析显示,基因组中没有插入或缺失突变。SC221-M中的nar、nap、nir、nor及nos反硝化酶相关基因在基因组中呈簇状分布,且SC221-M的NirS和NosZ蛋白序列与DSM4166和A1501的同源性较高,提示施氏假单胞菌SC221-M与A1501和DSM4166之间有着类似的反硝化机制。
研究了SC221-M及其复合菌制剂对草鱼养殖水体水化学指标的影响。试验设置了5个不同的试验组,分别在1400L水体中添加OCFU/ml SC221-M、1×105CFU/ml SC221-M、1×105CFU/ml蜡样芽孢杆菌BSC24、1×105CFU/ml的复合菌粉(SC221-M与BSC24等比例混合而成)和3×105CFU/ml的复合菌粉组。经过9d的饲养试验发现,与对照组相比,活菌制剂组水体中的TDS、氨氮、亚硝酸盐、总无机氮、总氮、COD、BOD等水化学指标有不同程度的降低,且复合菌的效果要明显好于单一菌剂。该结果说明有益微生物之间的相互协同作用能有效改良草鱼养殖水体的水质。
采集草鱼养殖试验第9d的对照组、BSC24组、SC221-M组和混合菌组的水样,提取细菌总DNA,利用16S rDNA V3引物对各样品进行PCR扩增,再使用Roche454GS FLX Titanium测序平台进行测序,并采用Qiime、Megan、Cytoscape等软件对所测序列进行分析。结果显示,复合菌组的多样性最为丰富。对照组、BSC24组、SC221-M组和复合菌组的细菌都是以变形菌门为主。从UPGMA聚类分析的结果看,各样品均按照不同的处理聚在不同的分支下。结果表明,添加益生菌可显著地改变养殖水体中的微生物群落结构,复合菌组的变化最为明显,水体微生物群落结构的改变与草鱼养殖水体水化学指标的变化相关。
In order to reduce the pollutants such as ammonium and nitrite in aquaculture water for grass carp, an isolate of denitrifying bacteria Pseudomonas stutzeri SC221was obtained by directional enrichment. After cobalt-60radiation, the nitrite removal capacity of the strain could be increased from50mg/L to100mg/L. It was named as P. stutzeri SC221-M after such radiation. Following researches on the effect of nitrogen sources, carbon sources, ratio of carbon to nitrogen (C/N) and temperature on the growth, denitrification rate and denitrification gene expression of SC221-M indicated that the best condition for the growth and denitrification of SC221-M was sodium nitrite as nitrogen source, sodium citrate as carbon source, C/N=4-8and at20-35℃. As the results shown, environmental factors could affect the growth and denitrification of SC221-M, and there was certain correlation between denitrification and nirS and nosZ gene expression.
Illumina Hiseq2000sequencing platform was used to obtain the complete genome sequence for SC221-M. Based on the assemble result, it was found that the genome size was4,545,027bp with64.01%GC content, the number of scaffold was131with399contig. Comparing with reference strain DSM4166, InDel was not appeared and there were60,641SNPs, including43,396synonymous mutations and10,743non-synonymous mutations in the genome of SC221-M. Through phylogenetic analysis, it was discovered that NirS and NosZ sequences of SC221-M were closely related to those of A1501and DSM4166, indicating that the strains showed a similar denitrification mechanism.
The effect of SC221-M and its mixed microbial preparation on hydrochemical indexes of the aquaculture water for grass carp was studied. There were five groups, including addition of0CFU/ml microbial preparation,1×105CFU/ml SC221-M,1×105CFU/ml Bacillus cereus BSC24,1×105CFU/ml mixed preparation (SC221-M: BSC24=1:1) and3×105CFU/ml mixed preparation in15PE buckets containing1400L water. It was found that addition of the microbial preparation could decrease the level of hydrochemical indexes, including TDS, ammonium, nitrite, total inorganic nitrogen, total nitrogen, COD and BOD, and the effect for mixed microbial preparation was better than that for SC221-M or BSC24preparation. The results indicated that the interaction between SC221-M and BSC24played an important role in improving water quality.
Water samples were collected from the control and treatment groups after9-day feeding. Bacterial genomic DNA was extracted and the V3region of16S rDNA was amplified by PCR. After the use of Roche454GS FLX Titanium to sequence the PCR products as well as Qiime, Megan and Cytoscapes to analyze DNA sequences, the results revealed that in each group, the most abundant phyla were Proteobacteria. The microbial diversity was increased when adding the mixed preparation and all samples were in different branches in accordance with the treatment through UPGMA cluster analysis. As is shown, microbial community structure in the water was significantly changed after adding microbial preparation, and there was a close relationship between microbial community structure and hydrochemical indexes.
通过Illumina Hiseq2000平台测序和序列组装,发现施氏假单胞菌SC221-M的基因组大小为4545027bp,GC含量为64.01%,共131个scaffold,399个contig。与参考菌株DSM4166的基因组进行比对,发现SC221-M基因组共有60641个SNP,基因区内的同义突变为43396个,非同义突变为10743个。InDel分析显示,基因组中没有插入或缺失突变。SC221-M中的nar、nap、nir、nor及nos反硝化酶相关基因在基因组中呈簇状分布,且SC221-M的NirS和NosZ蛋白序列与DSM4166和A1501的同源性较高,提示施氏假单胞菌SC221-M与A1501和DSM4166之间有着类似的反硝化机制。
研究了SC221-M及其复合菌制剂对草鱼养殖水体水化学指标的影响。试验设置了5个不同的试验组,分别在1400L水体中添加OCFU/ml SC221-M、1×105CFU/ml SC221-M、1×105CFU/ml蜡样芽孢杆菌BSC24、1×105CFU/ml的复合菌粉(SC221-M与BSC24等比例混合而成)和3×105CFU/ml的复合菌粉组。经过9d的饲养试验发现,与对照组相比,活菌制剂组水体中的TDS、氨氮、亚硝酸盐、总无机氮、总氮、COD、BOD等水化学指标有不同程度的降低,且复合菌的效果要明显好于单一菌剂。该结果说明有益微生物之间的相互协同作用能有效改良草鱼养殖水体的水质。
采集草鱼养殖试验第9d的对照组、BSC24组、SC221-M组和混合菌组的水样,提取细菌总DNA,利用16S rDNA V3引物对各样品进行PCR扩增,再使用Roche454GS FLX Titanium测序平台进行测序,并采用Qiime、Megan、Cytoscape等软件对所测序列进行分析。结果显示,复合菌组的多样性最为丰富。对照组、BSC24组、SC221-M组和复合菌组的细菌都是以变形菌门为主。从UPGMA聚类分析的结果看,各样品均按照不同的处理聚在不同的分支下。结果表明,添加益生菌可显著地改变养殖水体中的微生物群落结构,复合菌组的变化最为明显,水体微生物群落结构的改变与草鱼养殖水体水化学指标的变化相关。
In order to reduce the pollutants such as ammonium and nitrite in aquaculture water for grass carp, an isolate of denitrifying bacteria Pseudomonas stutzeri SC221was obtained by directional enrichment. After cobalt-60radiation, the nitrite removal capacity of the strain could be increased from50mg/L to100mg/L. It was named as P. stutzeri SC221-M after such radiation. Following researches on the effect of nitrogen sources, carbon sources, ratio of carbon to nitrogen (C/N) and temperature on the growth, denitrification rate and denitrification gene expression of SC221-M indicated that the best condition for the growth and denitrification of SC221-M was sodium nitrite as nitrogen source, sodium citrate as carbon source, C/N=4-8and at20-35℃. As the results shown, environmental factors could affect the growth and denitrification of SC221-M, and there was certain correlation between denitrification and nirS and nosZ gene expression.
Illumina Hiseq2000sequencing platform was used to obtain the complete genome sequence for SC221-M. Based on the assemble result, it was found that the genome size was4,545,027bp with64.01%GC content, the number of scaffold was131with399contig. Comparing with reference strain DSM4166, InDel was not appeared and there were60,641SNPs, including43,396synonymous mutations and10,743non-synonymous mutations in the genome of SC221-M. Through phylogenetic analysis, it was discovered that NirS and NosZ sequences of SC221-M were closely related to those of A1501and DSM4166, indicating that the strains showed a similar denitrification mechanism.
The effect of SC221-M and its mixed microbial preparation on hydrochemical indexes of the aquaculture water for grass carp was studied. There were five groups, including addition of0CFU/ml microbial preparation,1×105CFU/ml SC221-M,1×105CFU/ml Bacillus cereus BSC24,1×105CFU/ml mixed preparation (SC221-M: BSC24=1:1) and3×105CFU/ml mixed preparation in15PE buckets containing1400L water. It was found that addition of the microbial preparation could decrease the level of hydrochemical indexes, including TDS, ammonium, nitrite, total inorganic nitrogen, total nitrogen, COD and BOD, and the effect for mixed microbial preparation was better than that for SC221-M or BSC24preparation. The results indicated that the interaction between SC221-M and BSC24played an important role in improving water quality.
Water samples were collected from the control and treatment groups after9-day feeding. Bacterial genomic DNA was extracted and the V3region of16S rDNA was amplified by PCR. After the use of Roche454GS FLX Titanium to sequence the PCR products as well as Qiime, Megan and Cytoscapes to analyze DNA sequences, the results revealed that in each group, the most abundant phyla were Proteobacteria. The microbial diversity was increased when adding the mixed preparation and all samples were in different branches in accordance with the treatment through UPGMA cluster analysis. As is shown, microbial community structure in the water was significantly changed after adding microbial preparation, and there was a close relationship between microbial community structure and hydrochemical indexes.
引文
Achtman M. (2012). "Insights from genomic comparisons of genetically monomorphic bacterial pathogens." Philos Trans R Soc Lond B Biol Sci 367 (1590):860-867.
Alvarez M. L., Ai J., Zumft W., Sanders-Loehr J. and Dooley D. M. (2001). "Characterization of the copper-sulfur chromophores in nitrous oxide reductase by resonance raman spectroscopy:evidence for sulfur coordination in the catalytic cluster." J Am Chem Soc 123 (4):576-587.
Andersson A. F., Lindberg M., Jakobsson H., Backhed F., Nyren P. and Engstrand L. (2008). "Comparative analysis of human gut microbiota by barcoded pyrosequencing." PLoS One 3 (7):e2836.
Arillo A., Margiocco C., Melodia F., Mensi P. and Schenone G. (1981). "Ammonia toxicity mechanism in fish:studies on rainbow trout (Salmo gairdneri Rich)." Ecotoxicol Environ Saf 5 (3):316-328.
Axtell N. R., Sternberg S. P. and Claussen K. (2003). "Lead and nickel removal using Microspora and Lemna minor." Bioresour Technol 89 (1):41-48.
Bedzyk L., Wang T. and Ye R. W. (1999). "The periplasmic nitrate reductase in Pseudomonas sp. strain G-179 catalyzes the first step of denitrification." J Bacteriol 181 (9):2802-2806.
Bell L. C., Richardson D. J. and Ferguson S. J. (1990). "Periplasmic and membrane-bound respiratory nitrate reductases in Thiosphaera pantotropha. The periplasmic enzyme catalyzes the first step in aerobic denitrification." FEBS Lett 265 (1-2):85-87.
Benson G. (1999). "Tandem repeats finder:a program to analyze DNA sequences." Nucleic Acids Res 27 (2):573-580.
Blumle S. and Zumft W. G. (1991). "Respiratory nitrate reductase from denitrifying Pseudomonas stutzeri, purification, properties and target of proteolysis." Biochimica Et Biophysica Acta (BBA)-Bioenergetics 1057 (1):102-108.
Bonnett S. A., Blackwell M. S., Leah R., Cook V., O'Connor M. and Maltby E. (2013). "Temperature response of denitrification rate and greenhouse gas production in agricultural river marginal wetland soils." Geobiology 11 (3):252-267.
Bosch R., Garcia-Valdes E. and Moore E. R. (1999). "Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10." Gene 236 (1):149-157.
Bosch R., Garcia-Valdes E. and Moore E. R. (2000). "Complete nucleotide sequence and evolutionary significance of a chromosomally encoded naphthalene-degradation lower pathway from Pseudomonas stutzeri AN10." Gene 245 (1):65-74.
Bosch R., Moore E. R., Garcia-Valdes E. and Pieper D. H. (1999). "NahW, a novel, inducible salicylate hydroxylase involved in mineralization of naphthalene by Pseudomonas stutzeri AN10." J Bacteriol 181 (8):2315-2322.
Bratvold D. and Browdy C. L. (2001). "Effects of sand sediment and vertical surfaces (AquaMatsTM) on production, water quality, and microbial ecology in an intensive Litopenaeus vannamei culture system." Aquaculture 195 (1-2):81-94.
Brunet-Galmes I., Busquets A., Pena A., Gomila M., Nogales B., Garcia-Valdes E., Lalucat J., Bennasar A. and Bosch R. (2012). "Complete genome sequence of the naphthalene-degrading bacterium Pseudomonas stutzeri AN 10 (CCUG 29243)." J Bacteriol 194 (23):6642-6643.
Bullock G. L., Summerfelt S. T., Noble A. C., Weber A. L., Durant M. D. and Hankins J. A. (1997). "Ozonation of a recirculating rainbow trout culture system-I. Effects on bacterial gill disease and heterotrophic bacteria." Aquaculture 158 (1-2):43-55.
Burri R. and Stutzer A. (1895). "Ueber Nitrat zerstorende Bakterien und den durch dieselben bedingten Stickstoffverlust." Zentbl. Bakteriol. Parasitenkd. Abt. Ⅱ 1: 257-265.
Busquets A., Pena A., Gomila M., Bosch R., Nogales B., Garcia-Valdes E., Lalucat J. and Bennasar A. (2012). "Genome sequence of Pseudomonas stutzeri strain JM300 (DSM 10701), a soil isolate and model organism for natural transformation." J Bacteriol 194 (19):5477-5478.
Busquets A., Pena A., Gomila M., Mayol J., Bosch R., Nogales B., Garcia-Valdes E., Bennasar A. and Lalucat J. (2013). "Draft Genome Sequence of Pseudomonas stutzeri Strain B1SMN1, a Nitrogen-Fixing and Naphthalene-Degrading Strain Isolated from Wastewater." Genome Announc 1 (4).
Cai L., Liu G., Rensing C. and Wang G. (2009). "Genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils." BMC Microbiol 9:4.
Caporaso J. G., Bittinger K., Bushman F. D., DeSantis T. Z., Andersen G. L. and Knight R. (2010). "PyNAST:a flexible tool for aligning sequences to a template alignment." Bioinformatics 26 (2):266-267.
Caporaso J. G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F. D., Costello E. K., Fierer N., Pena A. G., Goodrich J. K., Gordon J. I., Huttley G. A., Kelley S. T., Knights D., Koenig J. E., Ley R. E., Lozupone C. A., McDonald D., Muegge B. D., Pirrung M., Reeder J., Sevinsky J. R., Turnbaugh P. J., Walters W. A., Widmann J., Yatsunenko T., Zaneveld J. and Knight R. (2010). "QIIME allows analysis of high-throughput community sequencing data." Nat Methods 7 (5): 335-336.
Chauhan A., Green S., Pathak A., Thomas J. and Venkatramanan R. (2013). "Whole-genome sequences of five oyster-associated bacteria show potential for crude oil hydrocarbon degradation." Genome Announcements 1 (5):e802-e813.
Chen M., Yan Y., Zhang W., Lu W., Wang J., Ping S. and Lin M. (2011). "Complete genome sequence of the type strain Pseudomonas stutzeri CGMCC 1.1803." J Bacteriol 193 (21):6095.
Chen P., Li J., Li Q. X., Wang Y., Li S., Ren T. and Wang L. (2012). "Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24." Bioresour Technol 116:266-270.
Cheung K. H. and Gu J. (2007). "Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential:A review." Int Biodeter Biodegr 59 (1):8-15.
Chiu Y. C. and Chung M. S. (2003). "Determination of optimal COD/nitrate ratio for biological denitrification." Int Biodeter Biodegr 51 (1):43-49.
Criddle C. S., DeWitt J. T., Grbic-Galic D. and McCarty P. L. (1990). "Transformation of carbon tetrachloride by Pseudomonas sp. strain KC under denitrification conditions." Appl Environ Microbiol 56 (11):3240-3246.
Dan N. P., Visvanathan C. and Basu B. (2003). "Comparative evaluation of yeast and bacterial treatment of high salinity wastewater based on biokinetic coefficients." Bioresour Technol 87 (1):51-56.
Daum M., Zimmer W., Papen H., Kloos K., Nawrath K. and Bothe H. (1998). "Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida." Curr Microbiol 37 (4): 281-288.
de Leeuw N., Dijkhuizen T., Hehir-Kwa J. Y., Carter N. P., Feuk L., Firth H. V., Kuhn R. M., Ledbetter D. H., Martin C. L., van Ravenswaaij-Arts C. M., Scherer S. W., Shams S., Van Vooren S., Sijmons R., Swertz M. and Hastings R. (2012). "Diagnostic interpretation of array data using public databases and internet sources." Hum Mutat.
Deng D., Mei C., Mai K., Tan B., Ai Q. and Ma H. (2013). "Effects of a yeast-based additive on growth and immune responses of white shrimp, Litopenaeus vannamei (Boone,1931), and aquaculture environment." Aquac Res 44 (9): 1348-1357.
DeSantis T. Z., Brodie E. L., Moberg J. P., Zubieta I. X., Piceno Y. M. and Andersen G. L. (2007). "High-density universal 16S rRNA microarray analysis reveals broader diversity than typical clone library when sampling the environment." Microbial Ecology 53 (3):371-383.
Edgar R. C. (2010). "Search and clustering orders of magnitude faster than BLAST." Bioinformatics 26 (19):2460-2461.
Farver O., Kroneck P. M., Zumft W. G. and Pecht I. (2003). "Allosteric control of internal electron transfer in cytochrome cdl nitrite reductase." Proc Natl Acad Sci U S a 100 (13):7622-7625.
Flanagan D. A., Gregory L. G., Carter J. P., Karakas-Sen A., Richardson D. J. and Spiro S. (1999). "Detection of genes for periplasmic nitrate reductase in nitrate respiring bacteria and in community DNA." FEMS Microbiol Lett 177 (2): 263-270.
Fuller R. (1989). "Probiotics in man and animals." J Appl Bacteriol 66 (5):365-378.
Garcia-Valdes E., Castillo M. M., Bennasar A., Guasp C., Cladera A. M., Bosch R., Engesser K. H. and Lalucat J. (2003). "Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro-and methylaromatics:a new genomovar within the species." Syst Appl Microbiol 26 (3):390-403.
Garland J. L. and Mills A. L. (1991). "Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization." Appl Environ Microbiol 57 (8): 2351-2359.
Grigoryeva T. V., Laikov A. V., Naumova R. P., Manolov A. I., Larin A. K., Karpova I. Y., Semashko T. A., Alexeev D. G., Kostryukova E. S., Muller R. and Govorun V. M. (2013). "Draft Genome of the Nitrogen-Fixing Bacterium Pseudomonas stutzeri Strain KOS6 Isolated from Industrial Hydrocarbon Sludge." Genome Announc 1 (1).
Hammouda O., Gaber A. and Abdel-Raouf N. (1995). "Microalgae and wastewater treatment." Ecotoxicol Environ Saf 31 (3):205-210.
Han R., Geller J. T., Yang L., Brodie E. L., Chakraborty R., Larsen J. T. and Beller H. R. (2010). "Physiological and transcriptional studies of Cr(VI) reduction under aerobic and denitrifying conditions by an aquifer-derived pseudomonad." Environ Sci Technol 44 (19):7491-7497.
Hao J., Ma C., Gao C., Qiu J., Wang M., Zhang Y., Cui X. and Xu P. (2007). "Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate." Biotechnol Lett 29 (1):105-110.
Harley K. (1990). "The role of biological control in the management of water hyacinth, Eichhornia crassipes." Biocontrol News and Information 11 (1):11-22.
Henderson S. L., Dandie C. E., Goyer C., Patten C., Zebarth B. J., Burton D. L., Trevors J. T. and Gilkes R. J. (2010). Glucose effects on denitrifier abundance, denitrification gene mRNA levels, and denitrification activity in an anoxic soil microcosm. Proceedings of the 19th World Congress of Soil Science:Soil solutions for a changing world, Brisbane, Australia,1-6 August 2010., International Union of Soil Sciences (IUSS), c/o Institut fur Bodenforschung, Universitat fur Bodenkultur.
Hettmann T., Anemuller S., Borcherding H., Mathe L., Steinrucke P. and Diekmann S. (2003). "Pseudomonas stutzeri soluble nitrate reductase alphabeta-subunit is a soluble enzyme with a similar electronic structure at the active site as the inner membrane-bound alphabetagamma holoenzyme." FEBS Lett 534 (1-3): 143-150.
Hu Z., Zhang J., Li S. and Xie H. (2013). "Impact of carbon source on nitrous oxide emission from anoxic/oxic biological nitrogen removal process and identification of its emission sources." Environ Sci Pollut Res Int 20 (2): 1059-1069.
Huson D. H., Mitra S., Ruscheweyh H. J., Weber N. and Schuster S. C. (2011). "Integrative analysis of environmental sequences using MEGAN4." Genome Res 21 (9):1552-1560.
Jiang T., Gao C., Su F., Zhang W., Hu C., Dou P., Zheng Z., Tao F., Ma C. and Xu P. (2012). "Genome sequence of Pseudomonas stutzeri SDM-LAC, a typical strain for studying the molecular mechanism of lactate utilization." J Bacteriol 194 (4): 894-895.
Joo S. and Foldenyi R. (2012). "Removal of dissolved organic matter (DOM) from water with activated carbon and effective microorganisms." Water Sci techonol-Water Supply 12 (1):65-71.
Kemp P. F. and Aller J. Y. (2004). "Bacterial diversity in aquatic and other environments:what 16S rDNA libraries can tell us." FEMS Microbiol Ecol 47 (2):161-177.
Kim J. K., Park K. J., Cho K. S., Nam S. W., Park T. J. and Bajpai R. (2005). "Aerobic nitrification-denitrification by heterotrophic Bacillus strains." Bioresour Technol 96(17):1897-1906.
Kim M., Jeong S. Y., Yoon S. J., Cho S. J., Kim Y. H., Kim M. J., Ryu E. Y. and Lee S. J. (2008). "Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios." J Biosci Bioeng 106 (5):498-502.
Krzywonos M. and Seruga P. (2012). "Decolorization of Sugar Beet Molasses Vinasse, a High-Strength Distillery Wastewater, by Lactic Acid Bacteria." Pol J Environ Stud 21 (4):943-948.
Kurtz S., Phillippy A., Delcher A. L., Smoot M., Shumway M., Antonescu C. and Salzberg S. L. (2004). "Versatile and open software for comparing large genomes." Genome Biol 5 (2):R12.
Lalucat J., Bennasar A., Bosch R., Garcia-Valdes E. and Palleroni N. J. (2006). "Biology of Pseudomonas stutzeri." Microbiol Mol Biol Rev 70 (2):510-547.
Lei L. C., Li X. J. and Zhang X. W. (2008). "Ammonium removal from aqueous solutions using microwave-treated natural Chinese zeolite." Sep Purif Technol 58 (3):359-366.
Li A., Gai Z., Cui D., Ma F., Yang J., Zhang X., Sun Y. and Ren N. (2012). "Genome sequence of a highly efficient aerobic denitrifying bacterium, Pseudomonas stutzeri T13." J Bacteriol 194 (20):5720.
Li R., Li Y., Fang X., Yang H., Wang J., Kristiansen K. and Wang J. (2009). "SNP detection for massively parallel whole-genome resequencing." Genome Res 19 (6):1124-1132.
Li R., Li Y., Kristiansen K. and Wang J. (2008). "SOAP:short oligonucleotide alignment program." Bioinformatics 24 (5):713-714.
Li R., Yu C., Li Y., Lam T. W., Yiu S. M., Kristiansen K. and Wang J. (2009). "SOAP2:an improved ultrafast tool for short read alignment." Bioinformatics 25 (15):1966-1967.
Li R., Zhu H., Ruan J., Qian W., Fang X., Shi Z., Li Y., Li S., Shan G., Kristiansen K., Li S., Yang H., Wang J. and Wang J. (2010). "De novo assembly of human genomes with massively parallel short read sequencing." Genome Res 20 (2): 265-272.
Li W. F., Deng B., Cui Z. W., Fu L. Q., Chen N. N., Zhou X. X., Shen W. Y. and Yu D. Y. (2012). "Several Indicators of Immunity and Antioxidant Activities Improved in Grass Carp Given a Diet Containing Bacillus Additive." J Anim Vet Adv 11 (14):2392-2397.
Li X., Gong J., Hu Y., Cai L., Johnstone L., Grass G., Rensing C. and Wang G. (2012). "Genome sequence of the moderately halotolerant, arsenite-oxidizing bacterium Pseudomonas stutzeri TS44." J Bacteriol 194 (16):4473-4474.
Liu X. R., Gai Z. H., Tao F., Yu H., Tang H. Z. and Xu P. (2012). "Genome Sequences of Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R, Two Efficient Carbazole-Degrading Strains." J Bacteriol 194 (20):5701-5702.
Loman N. J., Constantinidou C., Chan J. Z., Halachev M., Sergeant M., Penn C. W., Robinson E. R. and Pallen M. J. (2012). "High-throughput bacterial genome sequencing:an embarrassment of choice, a world of opportunity." Nat Rev Microbiol 10 (9):599-606.
Lorenz M. G. and Sikorski J. (2000). "The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri." Microbiology 146 Pt 12:3081-3090.
Lozupone C. and Knight R. (2005). "UniFrac:a new phylogenetic method for comparing microbial communities." Appl Environ Microbiol 71 (12): 8228-8235.
Miller M. N., Zebarth B. J., Dandie C. E., Burton D. L., Goyer C. and Trevors J. T. (2008). "Crop residue influence on denitrification, N2O emissions and denitrifier community abundance in soil." Soil Biol Biochem 40 (10):2553-2562.
Mires D. (1995). "Aquaculture and the aquatic environment:Mutual impact and preventive management." Isr J Aquacult-Bamid 47 (3-4):163-172.
Muyzer G., de Waal E. C. and Uitterlinden A. G. (1993). "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA." Appl Environ Microbiol 59 (3):695-700.
Pace N. R., Tahl D. A., Lane D. J. and Olsen G. J. (1986). "The analysis of natural microbial populations by ribosomal RNA sequences." Adv Microb Ecol 9:1-55.
Padhi S. K., Tripathy S., Sen R., Mahapatra A. S., Mohanty S. and Maiti N. K. (2013). "Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater." Int Biodeter Biodegr 78:67-73.
Pena A., Busquets A., Gomila M., Bosch R., Nogales B., Garcia-Valdes E., Lalucat J. and Bennasar A. (2012). "Draft genome of Pseudomonas stutzeri strain ZoBell (CCUG 16156), a marine isolate and model organism for denitrification studies." J Bacteriol 194 (5):1277-1278.
Pena A., Busquets A., Gomila M., Mayol J., Bosch R., Nogales B., Garcia-Valdes E., Bennasar A. and Lalucat J. (2013). "Draft Genome of Pseudomonas stutzeri Strain NF13, a Nitrogen Fixer Isolated from the Galapagos Rift Hydrothermal Vent." Genome Announc 1 (2):e11313.
Polprasert C. and Khatiwada N. R. (1998). "An integrated kinetic model for water hyacinth ponds used for wastewater treatment." Water Res 32 (1):179-185.
Price M. N., Dehal P. S. and Arkin A. P. (2010). "FastTree 2--approximately maximum-likelihood trees for large alignments." PLoS One 5 (3):e9490.
Rediers H., Bonnecarrere V., Rainey P. B., Hamonts K., Vanderleyden J. and De Mot R. (2003). "Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15." Appl Environ Microbiol 69 (11):6864-6874.
Robertson L. A. and Kuenen J. G. (1984). "Aerobic denitrification:a controversy revived." Arch Microbiol 139 (4):351-354.
Saleh-Lakha S., Shannon K. E., Henderson S. L., Goyer C., Trevors J. T., Zebarth B. J. and Burton D. L. (2009). "Effect of pH and temperature on denitrification gene expression and activity in Pseudomonas mandelii." Appl Environ Microbiol 75 (12):3903-3911.
Saleh-Lakha S., Shannon K. E., Henderson S. L., Zebarth B. J., Burton D. L., Goyer C. and Trevors J. T. (2009). "Effect of nitrate and acetylene on nirS, cnorB, and nosZ expression and denitrification activity in Pseudomonas mandelii." Appl Environ Microbiol 75 (15):5082-5087.
Schmid C. W. (1996). "Alu:structure, origin, evolution, significance and function of one-tenth of human DNA." Prog Nucleic Acid Res Mol Biol 53:283-319.
Schveitzer R., Arantes R., Baloi M. F., Costodio P. F. S., Arana L. V., Seiffert W. Q. and Andreatta E. R. (2013). "Use of artificial substrates in the culture of Litopenaeus vannamei (Biofloc System) at different stocking densities:Effects on microbial activity, water quality and production rates." Aquacult Eng 54: 93-103.
Sekar T. and Aranthathi S. (2008). "Comparision of Phytoremediation with Bioremediation for Atrazine Removal From Waste Water." Int J Appl Environ Sci 3 (3).
Sepulveda-Torres L. C., Rajendran N., Dybas M. J. and Criddle C. S. (1999). "Generation and initial characterization of Pseudomonas stutzeri KC mutants with impaired ability to degrade carbon tetrachloride." Arch Microbiol 171 (6): 424-429.
Smoot M. E., Ono K., Ruscheinski J., Wang P. L. and Ideker T. (2011). "Cytoscape 2.8:new features for data integration and network visualization." Bioinformatics 27 (3):431-432.
Sneath P. H. A., Mair N. S., Sharpe M. E. and Holt J. G. (1986). Bergey's manual of systematic bacteriology. Volume 2., Williams & Wilkins.
Song Z. Z., Wang L., Jin M. and Su Y. H. (2011). "Alternanthera philoxeroides might be used for bioremediating heavy metal contaminated soil." Genomics Appl Biol 30 (5):614-619.
Sood A., Uniyal P. L., Prasanna R. and Ahluwalia A. S. (2012). "Phytoremediation potential of aquatic macrophyte, Azolla." Ambio 41 (2):122-137.
Stanford G., Dzienia S. and Vander Pol R. A. (1975). "Effect of temperature on denitrification rate in soils." Soil Sci Soc Am J 39 (5):867-870.
Sumino T., Nakamura H., Mori N., Kawaguchi Y. and Tada M. (1992). "Immobilization of nitrifying bacteria in porous pellets of urethane gel for removal of ammonium nitrogen from waste-water." Appl Microbiol Biot 36 (4): 556-560.
Tilak K. S., Veeraiah K. and Raju J. M. (2007). "Effects of ammonia, nitrite and nitrate on hemoglobin content and oxygen consumption of freshwater fish, Cyprinus carpio (Linnaeus)." J Environ Biol 28 (1):45-47.
Wang Q., Garrity G. M., Tiedje J. M. and Cole J. R. (2007). "Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy." Appl Environ Microbiol 73 (16):5261-5267.
Weeg-Aerssens E., Wu W. S., Ye R. W., Tiedje J. M. and Chang C. K. (1991). "Purification of cytochrome cdl nitrite reductase from Pseudomonas stutzeri JM300 and reconstitution with native and synthetic heme dl." J Biol Chem 266 (12):7496-7502.
Welander U. and Mattiasson B. (2003). "Denitrification at low temperatures using a suspended carrier biofilm process." Water Res 37 (10):2394-2398.
White D. C., Davis W. M., Nickels J. S., King J. D. and Bobbie R. J. (1979). "Determination of the sedimentary microbial biomass by extractible lipid phosphate." Oecologia 40 (1):51-62.
Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W., Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y., Li D., Lin Z., Wang Y., Elmerich C., Lin M. and Jin Q. (2008). "Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501." Proc Natl Acad Sci U S a 105 (21):7564-7569.
Yang Q., Yang M., Zhang S. and Lv W. (2005). "Treatment of wastewater from a monosodium glutamate manufacturing plant using successive yeast and activated sludge systems." Process Biochem 40 (7):2483-2488.
Yao S., Ni J., Ma T. and Li C. (2013). "Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp. HA2." Bioresour Technol 139:80-86.
Yu H., Yuan M., Lu W., Yang J., Dai S., Li Q., Yang Z., Dong J., Sun L., Deng Z., Zhang W., Chen M., Ping S., Han Y., Zhan Y., Yan Y., Jin Q. and Lin M. (2011). "Complete genome sequence of the nitrogen-fixing and rhizosphere-associated bacterium Pseudomonas stutzeri strain DSM4166." J Bacteriol 193 (13):3422-3423.
Zawadzka A. M., Crawford R. L. and Paszczynski A. J. (2007). "Pyridine-2,6-bis(thiocarboxylic acid) produced by Pseudomonas stutzeri KC reduces chromium(VI) and precipitates mercury, cadmium, lead and arsenic." Biometals 20 (2):145-158.
Zhang J., Wu P., Hao B. and Yu Z. (2011). "Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001." Bioresour Technol 102 (21):9866-9869.
Zhang Q. L., Liu Y., Ai G. M., Miao L. L., Zheng H. Y. and Liu Z. P. (2012). "The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7." Bioresour Technol 108:35-44.
Zhang S. M., Li W. G., Zhang D. Y., Huang X. F., Qin W. and Gu J. (2013). "Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16." Desalin Water Treat(ahead-of-print):1-6.
Zhang S. M., Li W. G., Zhang D. Y., Huang X. F., W Q. and Q S. C. (2014). "Purification and Characterization of a Low-Temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16." Biomed Environ Sci(27).
Zhang X., Fu L., Deng B., Liang Q., Zheng J., Sun J., Zhu H., Peng L., Wang Y., Wenying S. and Li W. (2013). "Bacillus subtilis SC02 supplementation causes alterations of the microbial diversity in grass carp water." World J Microbiol Biotechnol 29 (9):1645-1653.
Zumft W. G. (2005). "Nitric oxide reductases of prokaryotes with emphasis on the respiratory, heme-copper oxidase type." J Inorg Biochem 99 (1):194-215.
Zumft W. G., Braun C. and Cuypers H. (1994). "Nitric oxide reductase from Pseudomonas stutzeri. Primary structure and gene organization of a novel bacterial cytochrome be complex." Eur J Biochem 219 (1-2):481-490.
蔡妙英,东秀珠(2001).常见细菌系统鉴定手册.北京,科学出版社.
蔡亚君,桂震,李锋,杨小俊(2011).”多功能水处理菌Pseudomonas stutzeri CDNl的筛选分离与性质鉴定.”湖北农业科学50(5)：908-910.
陈会克(1995).”光合细菌及其应用.”河南水产(02)：9-10.
陈营,王福强,邵占涛,王斌(2006).”乳酸菌对牙鲆稚鱼养殖水体和肠道菌群的影响.”海洋水产研究27(3)：37-41.
樊慧俐(2009).斯氏假单胞菌A1501固氮岛中NifA依赖的未知功能基因转录特性研究,中国农业科学院.硕士：77.
范毛毛,夏磊,杨仲明,赵淑坤(2011).”高性能乳酸菌在草鱼养殖中的应用试验.”现代渔业科技产业(3)：6-8.
丰茂武,吴云海,冯仕训,吴云影(2008).”不同氮磷比对藻类生长的影响.”生态环境17(5)：1759-1763.
冯俊荣,陈营,付学军,王爱敏(2005).”微生态制剂对养殖水体水质条件的影响.”海洋湖沼通报(4)：104-108.
付保荣,曹向宇,冷阳,顾继光,马汐平,李法云,杜麟(2008).”光合细菌对水产养殖水质和水生生物的影响.”生态科学27(2)：102-106.
付殿国,徐明(2005).”微生态制剂.”吉林畜牧兽医(4)：13-15.
国家环保局水和废水监测分析方法编委会(2002).水和废水检测分析方法(第四版).北京,中国环境科学出版.
国家环境保护局(1989).中国渔业水质标准GB11607-89.北京,中国标准出版社.
侯树宇,张清敏,多淼,王兰,钟欢,任富海(2004).”微生态复合菌制剂在对虾养殖中的应用研究.”农业环境科学学报23(5)：904-907.
胡金星,汤江武,王新,姚晓红,吴逸飞,葛向阳(2012).”一株反硝化细菌的鉴定、功能基因检测及其反硝化特性.”环境科学与技术(12)：27-32.
解志红,杨毅,平淑珍,陈明,张维,陆伟,徐玉泉,刘洪娟,王国英,Elmerich C.,林敏(2005).”利用酵母双杂交系统研究斯氏假单胞菌固氮调节蛋白NifL和NifA的相互作用.”科学通报50(1)：97-99.
孔庆鑫,李君文(2004).”微生物好氧反硝化脱氮作用研究进展.”环境与健康杂志21(3)：189-191.
孔庆鑫,李君文,王新为,金敏,古长庆(2005).“一种新的好氧反硝化菌筛选方法的建立及新菌株的发现.”应用与环境生物学报11(2)：222-225.
兰亚红(2008).施氏假单胞杆菌的毒死蜱降解酶的基本特性及其制剂的研究,中国农业科学院.
李超波,李文明,杨文华,李海星,刘晓华,曹郁生(2012).“黄曲霉毒素B1降解菌的分离鉴定及其降解特性.”微生物学报52(9)：1129-1136.
李明春,姜恒,侯文强,邢来君(1998,).“酵母菌对重金属离子吸附的研究.”菌物系统(04)：80-86.
李卫芬,傅罗琴,邓斌,陈南南,周绪霞(2011).“1株好氧反硝化菌的分离鉴定及反硝化特性研究.”环境科学32(8)：2403-2408.
李文明,杨文华,李海星,刘晓华,曹郁生(2013).”施氏假单胞菌F4对黄曲霉毒素B1的酶解作用及其降解产物的初步分析.”食品与发酵工业39(2)：11-16.
林娜,郭楚玲,柯林,党志(2010).”富营养化池塘中好氧反硝化菌的分布及脱氮研究.”中国科技论文在线5(5)：369-372,376.
刘慧玲,张战锋,李长玲,黄翔鹄,李瑞伟,林锡雄(2009).”光合细菌对罗非鱼鱼苗养殖水质及抗病力的影响.”渔业现代化36(2)：47-51.
刘万飞,王西亮,赵宇慧,曾瀞瑶,耿佳宁,胡松年(2011).”基于第二代测序技术的细菌基因组与转录组研究策略简介.”微生物学通报38(11)：1705-1714.
刘英霞,常显波,杨启霞(2010).“EM菌对养虾池水质的作用效果.”安徽农业科学38(13)：6891-6892.
马海军,赵振良,穆珂馨(2010).”复合微生态制剂在半封闭混养池塘中的应用.”大连海洋大学学报25(6)：556-559.
马展(2003).城市污水处理系统的菌群分析和nap基因的克隆表达,重庆医科大学
彭小珍,吉宏武,刘唤明,章超桦(2007).”施氏假单胞菌Pseudomonas stutzeri产 卡拉胶酶液体发酵条件优化.”食品研究与开发28(10)：7-11.
彭自然,臧维玲,高杨,江敏,徐桂荣,丁福江(2004).”氨和亚硝酸盐对凡纳滨对虾幼虾的毒性影响.”上海水产大学学报13(3)：274-278.
史德青,赵金生,杨金荣,侯影飞,孔瑛(2004).”施氏假单胞菌对二苯并噻吩的降解.”中国环境科学24(6)：730-733.
史建华,徐琴英,王韩信,李建忠,朱选才(2007).”生石灰在水产养殖中的应用.”水产科技情报34(2)：86-89.
宋蓉(2012).乳酸菌对养殖水体亚硝酸盐控制机理的研究,华中农业大学.
田凤蓉,刘英,吕文洲,呼世斌(2011).”酵母菌产乳化剂能力及其对含油废水降解性能的影响研究.”环境工程学报05(5)：1062-1066.
田功太,刘飞,段登选,杜兴华,张金路,陈述江,张明磊,王慧(2012).“EM菌对海参养殖水体主要污染物净化效果的研究.”山东农业大学学报(自然科学版)43(3)：381-386.
王彬彬,熊丽,郑永良,张志霞,童文羽,刘松,陈奕和,肖文精,刘德立(2008).”甲基对硫磷高效降解菌的分离鉴定及降解酶基因的克隆表达.”环境科学学报28(10)：1969-1975.
王春德,齐翠红,李朝霞(2012).”过氧化钙的净水增氧效果评估.”生命科学研究16(4)：345-349.
王峰慧,智利,魏巍,苏俊峰(2011).”微生态菌剂直投技术原位修复微污染水源水的试验研究.”给水排水37(1)：143-146.
王连峰,蔡祖聪(2004).”水分和温度对旱地红壤硝化活力和反硝化活力的影响.”土壤36(5)：543-546,560.
王瑞(2011).固氮施氏假单胞菌ACC脱氨酶基因acdS的功能鉴定,中国农业科学院.
王莹,周巧红,梁威,徐栋,蔡林林,陶敏,吴振斌(2010).”人工湿地高效好氧反硝化菌的分离鉴定及反硝化特性研究.”农业环境科学学报29(6)：1193-1198.
王有乐,雷兴龙,王亚变,杨勤(2010).”好氧反硝化菌筛选方法的研究进展.”安徽农业科学38(10)：5294-5296,5300.
王志盈,袁林江,彭党聪,刘超翔(2000).”内循环生物流化床硝化过程的选择特性研究.”中国给水排水16(4)：1-4.
韦璐,江敏,余根鼎,李世凯,戴习林,顾德平,胡伟国(2012).”凡纳滨对虾养殖塘叶绿素a与水质因子主成分多元线性回归分析.”中国水产科学19(4)：620-625.
吴鹏霞(2009).异养硝化细菌的分离、施氏假单胞菌YZN-001的鉴定及特性研究,华中农业大学.硕士：55.
谢航,邱宏端,林娟,陈朝洋(2005).”假丝酵母菌降解养殖水体氨氮的特性研究.”农业工程学报21(8)：142-145.
辛玉峰,曲晓华,袁梦冬,荆延德(2011).”一株异养硝化-反硝化不动杆菌的分离鉴定及脱氮活性.”微生物学报51(12)：1646-1654.
邢殿楼,霍堂斌,吴会民,刘靖,刘长发,雷衍之(2006).”总磷、总氮联合消化的测定方法.”大连水产学院学报21(3)：219-225.
熊小京,李博,林光辉,郑天凌,彭义亮(2011).”包埋固定化EM菌的氨氮去除特性研究.”环境卫生工程19(2)：39-41,44.
徐爱玲,张帅,张燕飞,历丽,杨宇,夏金兰(2008).”积累PHB菌种隐藏嗜酸菌DX1-1的诱变改良.”微生物学通报35(10)：1516-1521.
燕永亮(2005).斯氏假单胞菌(Pseudomonas stutzeri)A1501的基因组学研究,中国农业大学.
杨俊忠,倪砚,许尚营,徐可瀚,刘义,曾丽霞,刘德立(2010).”一株高效好氧反硝化菌的分离及特性.”微生物学通报37(11)：1594-1599.
杨小龙(2012).复合菌剂的研制及其对水产养殖污水的净化作用,南昌大学.
杨小龙,李文明,陈燕,曹郁生(2011).”一株好氧反硝化菌的分离鉴定及其除氮特性.”微生物学报51(8)：1062-1070.
杨益民(2011).叶绿素a荧光检测仪的设计与开发,浙江大学.
易力,汪洋,陈万光,张耀武(2011).”固定化浓缩光合细菌对养殖水环境的影响.”贵州农业科学39(6)：152-154.
殷芳芳,王淑莹,昂雪野,侯红勋,彭永臻,王伟(2009).”碳源类型对低温条件下生物反硝化的影响.”环境科学30(1)：108-113.
尹文林,沈锦玉,潘晓艺,姚嘉赞,徐洋,郝贵杰(2010).”复合硝化菌制剂对水质改良的应用效果.”水产养殖31(9)：12-17.
尹艳娥,沈新强,晁敏,全为民,王云龙(2009).”反硝化技术对模拟养殖池塘修复的研究.”农业环境科学学报28(8)：1727-1732.
余日清,贺锡勤(1992).”低pH对草鱼血液酸碱平衡的影响.”环境科学学报12(1)：112-118.
余雄奎(2008).固定化藻菌生物修复富营养化水体的模拟研究,武汉科技大学.
詹金星,支崇远,夏品华,马建荣,李海珍(2011).”水生植物净化污水的机理及研究进展.”西南农业学报24(1)：352-355.
张峰峰,谢凤行,赵玉洁,周可,李亚玲(2013).”一株反硝化细菌的分离鉴定及脱氮能力.”环境工程学报7(11)：4415-4420.
张凯,雷梦婕,胡国元,袁军,杨洋,章建国(2011).”好氧反硝化细菌WIT-1的分离鉴定及其脱氨氮特性.”武汉工程大学学报33(11)：14-18.
张秋磊(2008).固氮斯氏假单胞菌A1501鞭毛调控基fleQ的功能研究,中国农业科学院.
张小玲,袁科平,耿康,张玲,杨丽(2011).”好氧反硝化菌对水质和鱼体饲喂效果的影响研究.”水生态学杂志32(3)：114-119.
郑佳佳,张小平,胡彩虹,李卫芬(2013).”斯氏假单胞菌对草鱼养殖水体水质的调控作用.”渔业现代化(3)：5-9.
中华人民共和国农业部(2013).饲料添加剂品种目录.
周淑云(2006).毒死蜱高效降解菌的分离鉴定及其生物学特性研究,西南大学.
周鑫,王素英,宋霖霞(2010).”海水养殖含氮废水处理的复合微生态制剂研制.”微生物学通报37(4)：543-546.
朱红瑜(2007).屠宰废水生物处理低温菌种的筛选,吉林农业大学.
朱亮,汪岁羽,朱雪诞,邵孝侯,刘德有(2002).“EM菌富集培养及降解污水试验研究.”河海大学学报(自然科学版)30(2)：6-8.
朱明瑞,曹广斌,蒋树义,韩世成(2007).”工厂化水产养殖水体的pH值在线自动控制系统.”水产学报31(3)：335-342.
Alvarez M. L., Ai J., Zumft W., Sanders-Loehr J. and Dooley D. M. (2001). "Characterization of the copper-sulfur chromophores in nitrous oxide reductase by resonance raman spectroscopy:evidence for sulfur coordination in the catalytic cluster." J Am Chem Soc 123 (4):576-587.
Andersson A. F., Lindberg M., Jakobsson H., Backhed F., Nyren P. and Engstrand L. (2008). "Comparative analysis of human gut microbiota by barcoded pyrosequencing." PLoS One 3 (7):e2836.
Arillo A., Margiocco C., Melodia F., Mensi P. and Schenone G. (1981). "Ammonia toxicity mechanism in fish:studies on rainbow trout (Salmo gairdneri Rich)." Ecotoxicol Environ Saf 5 (3):316-328.
Axtell N. R., Sternberg S. P. and Claussen K. (2003). "Lead and nickel removal using Microspora and Lemna minor." Bioresour Technol 89 (1):41-48.
Bedzyk L., Wang T. and Ye R. W. (1999). "The periplasmic nitrate reductase in Pseudomonas sp. strain G-179 catalyzes the first step of denitrification." J Bacteriol 181 (9):2802-2806.
Bell L. C., Richardson D. J. and Ferguson S. J. (1990). "Periplasmic and membrane-bound respiratory nitrate reductases in Thiosphaera pantotropha. The periplasmic enzyme catalyzes the first step in aerobic denitrification." FEBS Lett 265 (1-2):85-87.
Benson G. (1999). "Tandem repeats finder:a program to analyze DNA sequences." Nucleic Acids Res 27 (2):573-580.
Blumle S. and Zumft W. G. (1991). "Respiratory nitrate reductase from denitrifying Pseudomonas stutzeri, purification, properties and target of proteolysis." Biochimica Et Biophysica Acta (BBA)-Bioenergetics 1057 (1):102-108.
Bonnett S. A., Blackwell M. S., Leah R., Cook V., O'Connor M. and Maltby E. (2013). "Temperature response of denitrification rate and greenhouse gas production in agricultural river marginal wetland soils." Geobiology 11 (3):252-267.
Bosch R., Garcia-Valdes E. and Moore E. R. (1999). "Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10." Gene 236 (1):149-157.
Bosch R., Garcia-Valdes E. and Moore E. R. (2000). "Complete nucleotide sequence and evolutionary significance of a chromosomally encoded naphthalene-degradation lower pathway from Pseudomonas stutzeri AN10." Gene 245 (1):65-74.
Bosch R., Moore E. R., Garcia-Valdes E. and Pieper D. H. (1999). "NahW, a novel, inducible salicylate hydroxylase involved in mineralization of naphthalene by Pseudomonas stutzeri AN10." J Bacteriol 181 (8):2315-2322.
Bratvold D. and Browdy C. L. (2001). "Effects of sand sediment and vertical surfaces (AquaMatsTM) on production, water quality, and microbial ecology in an intensive Litopenaeus vannamei culture system." Aquaculture 195 (1-2):81-94.
Brunet-Galmes I., Busquets A., Pena A., Gomila M., Nogales B., Garcia-Valdes E., Lalucat J., Bennasar A. and Bosch R. (2012). "Complete genome sequence of the naphthalene-degrading bacterium Pseudomonas stutzeri AN 10 (CCUG 29243)." J Bacteriol 194 (23):6642-6643.
Bullock G. L., Summerfelt S. T., Noble A. C., Weber A. L., Durant M. D. and Hankins J. A. (1997). "Ozonation of a recirculating rainbow trout culture system-I. Effects on bacterial gill disease and heterotrophic bacteria." Aquaculture 158 (1-2):43-55.
Burri R. and Stutzer A. (1895). "Ueber Nitrat zerstorende Bakterien und den durch dieselben bedingten Stickstoffverlust." Zentbl. Bakteriol. Parasitenkd. Abt. Ⅱ 1: 257-265.
Busquets A., Pena A., Gomila M., Bosch R., Nogales B., Garcia-Valdes E., Lalucat J. and Bennasar A. (2012). "Genome sequence of Pseudomonas stutzeri strain JM300 (DSM 10701), a soil isolate and model organism for natural transformation." J Bacteriol 194 (19):5477-5478.
Busquets A., Pena A., Gomila M., Mayol J., Bosch R., Nogales B., Garcia-Valdes E., Bennasar A. and Lalucat J. (2013). "Draft Genome Sequence of Pseudomonas stutzeri Strain B1SMN1, a Nitrogen-Fixing and Naphthalene-Degrading Strain Isolated from Wastewater." Genome Announc 1 (4).
Cai L., Liu G., Rensing C. and Wang G. (2009). "Genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils." BMC Microbiol 9:4.
Caporaso J. G., Bittinger K., Bushman F. D., DeSantis T. Z., Andersen G. L. and Knight R. (2010). "PyNAST:a flexible tool for aligning sequences to a template alignment." Bioinformatics 26 (2):266-267.
Caporaso J. G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F. D., Costello E. K., Fierer N., Pena A. G., Goodrich J. K., Gordon J. I., Huttley G. A., Kelley S. T., Knights D., Koenig J. E., Ley R. E., Lozupone C. A., McDonald D., Muegge B. D., Pirrung M., Reeder J., Sevinsky J. R., Turnbaugh P. J., Walters W. A., Widmann J., Yatsunenko T., Zaneveld J. and Knight R. (2010). "QIIME allows analysis of high-throughput community sequencing data." Nat Methods 7 (5): 335-336.
Chauhan A., Green S., Pathak A., Thomas J. and Venkatramanan R. (2013). "Whole-genome sequences of five oyster-associated bacteria show potential for crude oil hydrocarbon degradation." Genome Announcements 1 (5):e802-e813.
Chen M., Yan Y., Zhang W., Lu W., Wang J., Ping S. and Lin M. (2011). "Complete genome sequence of the type strain Pseudomonas stutzeri CGMCC 1.1803." J Bacteriol 193 (21):6095.
Chen P., Li J., Li Q. X., Wang Y., Li S., Ren T. and Wang L. (2012). "Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24." Bioresour Technol 116:266-270.
Cheung K. H. and Gu J. (2007). "Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential:A review." Int Biodeter Biodegr 59 (1):8-15.
Chiu Y. C. and Chung M. S. (2003). "Determination of optimal COD/nitrate ratio for biological denitrification." Int Biodeter Biodegr 51 (1):43-49.
Criddle C. S., DeWitt J. T., Grbic-Galic D. and McCarty P. L. (1990). "Transformation of carbon tetrachloride by Pseudomonas sp. strain KC under denitrification conditions." Appl Environ Microbiol 56 (11):3240-3246.
Dan N. P., Visvanathan C. and Basu B. (2003). "Comparative evaluation of yeast and bacterial treatment of high salinity wastewater based on biokinetic coefficients." Bioresour Technol 87 (1):51-56.
Daum M., Zimmer W., Papen H., Kloos K., Nawrath K. and Bothe H. (1998). "Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida." Curr Microbiol 37 (4): 281-288.
de Leeuw N., Dijkhuizen T., Hehir-Kwa J. Y., Carter N. P., Feuk L., Firth H. V., Kuhn R. M., Ledbetter D. H., Martin C. L., van Ravenswaaij-Arts C. M., Scherer S. W., Shams S., Van Vooren S., Sijmons R., Swertz M. and Hastings R. (2012). "Diagnostic interpretation of array data using public databases and internet sources." Hum Mutat.
Deng D., Mei C., Mai K., Tan B., Ai Q. and Ma H. (2013). "Effects of a yeast-based additive on growth and immune responses of white shrimp, Litopenaeus vannamei (Boone,1931), and aquaculture environment." Aquac Res 44 (9): 1348-1357.
DeSantis T. Z., Brodie E. L., Moberg J. P., Zubieta I. X., Piceno Y. M. and Andersen G. L. (2007). "High-density universal 16S rRNA microarray analysis reveals broader diversity than typical clone library when sampling the environment." Microbial Ecology 53 (3):371-383.
Edgar R. C. (2010). "Search and clustering orders of magnitude faster than BLAST." Bioinformatics 26 (19):2460-2461.
Farver O., Kroneck P. M., Zumft W. G. and Pecht I. (2003). "Allosteric control of internal electron transfer in cytochrome cdl nitrite reductase." Proc Natl Acad Sci U S a 100 (13):7622-7625.
Flanagan D. A., Gregory L. G., Carter J. P., Karakas-Sen A., Richardson D. J. and Spiro S. (1999). "Detection of genes for periplasmic nitrate reductase in nitrate respiring bacteria and in community DNA." FEMS Microbiol Lett 177 (2): 263-270.
Fuller R. (1989). "Probiotics in man and animals." J Appl Bacteriol 66 (5):365-378.
Garcia-Valdes E., Castillo M. M., Bennasar A., Guasp C., Cladera A. M., Bosch R., Engesser K. H. and Lalucat J. (2003). "Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro-and methylaromatics:a new genomovar within the species." Syst Appl Microbiol 26 (3):390-403.
Garland J. L. and Mills A. L. (1991). "Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization." Appl Environ Microbiol 57 (8): 2351-2359.
Grigoryeva T. V., Laikov A. V., Naumova R. P., Manolov A. I., Larin A. K., Karpova I. Y., Semashko T. A., Alexeev D. G., Kostryukova E. S., Muller R. and Govorun V. M. (2013). "Draft Genome of the Nitrogen-Fixing Bacterium Pseudomonas stutzeri Strain KOS6 Isolated from Industrial Hydrocarbon Sludge." Genome Announc 1 (1).
Hammouda O., Gaber A. and Abdel-Raouf N. (1995). "Microalgae and wastewater treatment." Ecotoxicol Environ Saf 31 (3):205-210.
Han R., Geller J. T., Yang L., Brodie E. L., Chakraborty R., Larsen J. T. and Beller H. R. (2010). "Physiological and transcriptional studies of Cr(VI) reduction under aerobic and denitrifying conditions by an aquifer-derived pseudomonad." Environ Sci Technol 44 (19):7491-7497.
Hao J., Ma C., Gao C., Qiu J., Wang M., Zhang Y., Cui X. and Xu P. (2007). "Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate." Biotechnol Lett 29 (1):105-110.
Harley K. (1990). "The role of biological control in the management of water hyacinth, Eichhornia crassipes." Biocontrol News and Information 11 (1):11-22.
Henderson S. L., Dandie C. E., Goyer C., Patten C., Zebarth B. J., Burton D. L., Trevors J. T. and Gilkes R. J. (2010). Glucose effects on denitrifier abundance, denitrification gene mRNA levels, and denitrification activity in an anoxic soil microcosm. Proceedings of the 19th World Congress of Soil Science:Soil solutions for a changing world, Brisbane, Australia,1-6 August 2010., International Union of Soil Sciences (IUSS), c/o Institut fur Bodenforschung, Universitat fur Bodenkultur.
Hettmann T., Anemuller S., Borcherding H., Mathe L., Steinrucke P. and Diekmann S. (2003). "Pseudomonas stutzeri soluble nitrate reductase alphabeta-subunit is a soluble enzyme with a similar electronic structure at the active site as the inner membrane-bound alphabetagamma holoenzyme." FEBS Lett 534 (1-3): 143-150.
Hu Z., Zhang J., Li S. and Xie H. (2013). "Impact of carbon source on nitrous oxide emission from anoxic/oxic biological nitrogen removal process and identification of its emission sources." Environ Sci Pollut Res Int 20 (2): 1059-1069.
Huson D. H., Mitra S., Ruscheweyh H. J., Weber N. and Schuster S. C. (2011). "Integrative analysis of environmental sequences using MEGAN4." Genome Res 21 (9):1552-1560.
Jiang T., Gao C., Su F., Zhang W., Hu C., Dou P., Zheng Z., Tao F., Ma C. and Xu P. (2012). "Genome sequence of Pseudomonas stutzeri SDM-LAC, a typical strain for studying the molecular mechanism of lactate utilization." J Bacteriol 194 (4): 894-895.
Joo S. and Foldenyi R. (2012). "Removal of dissolved organic matter (DOM) from water with activated carbon and effective microorganisms." Water Sci techonol-Water Supply 12 (1):65-71.
Kemp P. F. and Aller J. Y. (2004). "Bacterial diversity in aquatic and other environments:what 16S rDNA libraries can tell us." FEMS Microbiol Ecol 47 (2):161-177.
Kim J. K., Park K. J., Cho K. S., Nam S. W., Park T. J. and Bajpai R. (2005). "Aerobic nitrification-denitrification by heterotrophic Bacillus strains." Bioresour Technol 96(17):1897-1906.
Kim M., Jeong S. Y., Yoon S. J., Cho S. J., Kim Y. H., Kim M. J., Ryu E. Y. and Lee S. J. (2008). "Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios." J Biosci Bioeng 106 (5):498-502.
Krzywonos M. and Seruga P. (2012). "Decolorization of Sugar Beet Molasses Vinasse, a High-Strength Distillery Wastewater, by Lactic Acid Bacteria." Pol J Environ Stud 21 (4):943-948.
Kurtz S., Phillippy A., Delcher A. L., Smoot M., Shumway M., Antonescu C. and Salzberg S. L. (2004). "Versatile and open software for comparing large genomes." Genome Biol 5 (2):R12.
Lalucat J., Bennasar A., Bosch R., Garcia-Valdes E. and Palleroni N. J. (2006). "Biology of Pseudomonas stutzeri." Microbiol Mol Biol Rev 70 (2):510-547.
Lei L. C., Li X. J. and Zhang X. W. (2008). "Ammonium removal from aqueous solutions using microwave-treated natural Chinese zeolite." Sep Purif Technol 58 (3):359-366.
Li A., Gai Z., Cui D., Ma F., Yang J., Zhang X., Sun Y. and Ren N. (2012). "Genome sequence of a highly efficient aerobic denitrifying bacterium, Pseudomonas stutzeri T13." J Bacteriol 194 (20):5720.
Li R., Li Y., Fang X., Yang H., Wang J., Kristiansen K. and Wang J. (2009). "SNP detection for massively parallel whole-genome resequencing." Genome Res 19 (6):1124-1132.
Li R., Li Y., Kristiansen K. and Wang J. (2008). "SOAP:short oligonucleotide alignment program." Bioinformatics 24 (5):713-714.
Li R., Yu C., Li Y., Lam T. W., Yiu S. M., Kristiansen K. and Wang J. (2009). "SOAP2:an improved ultrafast tool for short read alignment." Bioinformatics 25 (15):1966-1967.
Li R., Zhu H., Ruan J., Qian W., Fang X., Shi Z., Li Y., Li S., Shan G., Kristiansen K., Li S., Yang H., Wang J. and Wang J. (2010). "De novo assembly of human genomes with massively parallel short read sequencing." Genome Res 20 (2): 265-272.
Li W. F., Deng B., Cui Z. W., Fu L. Q., Chen N. N., Zhou X. X., Shen W. Y. and Yu D. Y. (2012). "Several Indicators of Immunity and Antioxidant Activities Improved in Grass Carp Given a Diet Containing Bacillus Additive." J Anim Vet Adv 11 (14):2392-2397.
Li X., Gong J., Hu Y., Cai L., Johnstone L., Grass G., Rensing C. and Wang G. (2012). "Genome sequence of the moderately halotolerant, arsenite-oxidizing bacterium Pseudomonas stutzeri TS44." J Bacteriol 194 (16):4473-4474.
Liu X. R., Gai Z. H., Tao F., Yu H., Tang H. Z. and Xu P. (2012). "Genome Sequences of Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R, Two Efficient Carbazole-Degrading Strains." J Bacteriol 194 (20):5701-5702.
Loman N. J., Constantinidou C., Chan J. Z., Halachev M., Sergeant M., Penn C. W., Robinson E. R. and Pallen M. J. (2012). "High-throughput bacterial genome sequencing:an embarrassment of choice, a world of opportunity." Nat Rev Microbiol 10 (9):599-606.
Lorenz M. G. and Sikorski J. (2000). "The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri." Microbiology 146 Pt 12:3081-3090.
Lozupone C. and Knight R. (2005). "UniFrac:a new phylogenetic method for comparing microbial communities." Appl Environ Microbiol 71 (12): 8228-8235.
Miller M. N., Zebarth B. J., Dandie C. E., Burton D. L., Goyer C. and Trevors J. T. (2008). "Crop residue influence on denitrification, N2O emissions and denitrifier community abundance in soil." Soil Biol Biochem 40 (10):2553-2562.
Mires D. (1995). "Aquaculture and the aquatic environment:Mutual impact and preventive management." Isr J Aquacult-Bamid 47 (3-4):163-172.
Muyzer G., de Waal E. C. and Uitterlinden A. G. (1993). "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA." Appl Environ Microbiol 59 (3):695-700.
Pace N. R., Tahl D. A., Lane D. J. and Olsen G. J. (1986). "The analysis of natural microbial populations by ribosomal RNA sequences." Adv Microb Ecol 9:1-55.
Padhi S. K., Tripathy S., Sen R., Mahapatra A. S., Mohanty S. and Maiti N. K. (2013). "Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater." Int Biodeter Biodegr 78:67-73.
Pena A., Busquets A., Gomila M., Bosch R., Nogales B., Garcia-Valdes E., Lalucat J. and Bennasar A. (2012). "Draft genome of Pseudomonas stutzeri strain ZoBell (CCUG 16156), a marine isolate and model organism for denitrification studies." J Bacteriol 194 (5):1277-1278.
Pena A., Busquets A., Gomila M., Mayol J., Bosch R., Nogales B., Garcia-Valdes E., Bennasar A. and Lalucat J. (2013). "Draft Genome of Pseudomonas stutzeri Strain NF13, a Nitrogen Fixer Isolated from the Galapagos Rift Hydrothermal Vent." Genome Announc 1 (2):e11313.
Polprasert C. and Khatiwada N. R. (1998). "An integrated kinetic model for water hyacinth ponds used for wastewater treatment." Water Res 32 (1):179-185.
Price M. N., Dehal P. S. and Arkin A. P. (2010). "FastTree 2--approximately maximum-likelihood trees for large alignments." PLoS One 5 (3):e9490.
Rediers H., Bonnecarrere V., Rainey P. B., Hamonts K., Vanderleyden J. and De Mot R. (2003). "Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15." Appl Environ Microbiol 69 (11):6864-6874.
Robertson L. A. and Kuenen J. G. (1984). "Aerobic denitrification:a controversy revived." Arch Microbiol 139 (4):351-354.
Saleh-Lakha S., Shannon K. E., Henderson S. L., Goyer C., Trevors J. T., Zebarth B. J. and Burton D. L. (2009). "Effect of pH and temperature on denitrification gene expression and activity in Pseudomonas mandelii." Appl Environ Microbiol 75 (12):3903-3911.
Saleh-Lakha S., Shannon K. E., Henderson S. L., Zebarth B. J., Burton D. L., Goyer C. and Trevors J. T. (2009). "Effect of nitrate and acetylene on nirS, cnorB, and nosZ expression and denitrification activity in Pseudomonas mandelii." Appl Environ Microbiol 75 (15):5082-5087.
Schmid C. W. (1996). "Alu:structure, origin, evolution, significance and function of one-tenth of human DNA." Prog Nucleic Acid Res Mol Biol 53:283-319.
Schveitzer R., Arantes R., Baloi M. F., Costodio P. F. S., Arana L. V., Seiffert W. Q. and Andreatta E. R. (2013). "Use of artificial substrates in the culture of Litopenaeus vannamei (Biofloc System) at different stocking densities:Effects on microbial activity, water quality and production rates." Aquacult Eng 54: 93-103.
Sekar T. and Aranthathi S. (2008). "Comparision of Phytoremediation with Bioremediation for Atrazine Removal From Waste Water." Int J Appl Environ Sci 3 (3).
Sepulveda-Torres L. C., Rajendran N., Dybas M. J. and Criddle C. S. (1999). "Generation and initial characterization of Pseudomonas stutzeri KC mutants with impaired ability to degrade carbon tetrachloride." Arch Microbiol 171 (6): 424-429.
Smoot M. E., Ono K., Ruscheinski J., Wang P. L. and Ideker T. (2011). "Cytoscape 2.8:new features for data integration and network visualization." Bioinformatics 27 (3):431-432.
Sneath P. H. A., Mair N. S., Sharpe M. E. and Holt J. G. (1986). Bergey's manual of systematic bacteriology. Volume 2., Williams & Wilkins.
Song Z. Z., Wang L., Jin M. and Su Y. H. (2011). "Alternanthera philoxeroides might be used for bioremediating heavy metal contaminated soil." Genomics Appl Biol 30 (5):614-619.
Sood A., Uniyal P. L., Prasanna R. and Ahluwalia A. S. (2012). "Phytoremediation potential of aquatic macrophyte, Azolla." Ambio 41 (2):122-137.
Stanford G., Dzienia S. and Vander Pol R. A. (1975). "Effect of temperature on denitrification rate in soils." Soil Sci Soc Am J 39 (5):867-870.
Sumino T., Nakamura H., Mori N., Kawaguchi Y. and Tada M. (1992). "Immobilization of nitrifying bacteria in porous pellets of urethane gel for removal of ammonium nitrogen from waste-water." Appl Microbiol Biot 36 (4): 556-560.
Tilak K. S., Veeraiah K. and Raju J. M. (2007). "Effects of ammonia, nitrite and nitrate on hemoglobin content and oxygen consumption of freshwater fish, Cyprinus carpio (Linnaeus)." J Environ Biol 28 (1):45-47.
Wang Q., Garrity G. M., Tiedje J. M. and Cole J. R. (2007). "Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy." Appl Environ Microbiol 73 (16):5261-5267.
Weeg-Aerssens E., Wu W. S., Ye R. W., Tiedje J. M. and Chang C. K. (1991). "Purification of cytochrome cdl nitrite reductase from Pseudomonas stutzeri JM300 and reconstitution with native and synthetic heme dl." J Biol Chem 266 (12):7496-7502.
Welander U. and Mattiasson B. (2003). "Denitrification at low temperatures using a suspended carrier biofilm process." Water Res 37 (10):2394-2398.
White D. C., Davis W. M., Nickels J. S., King J. D. and Bobbie R. J. (1979). "Determination of the sedimentary microbial biomass by extractible lipid phosphate." Oecologia 40 (1):51-62.
Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W., Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y., Li D., Lin Z., Wang Y., Elmerich C., Lin M. and Jin Q. (2008). "Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501." Proc Natl Acad Sci U S a 105 (21):7564-7569.
Yang Q., Yang M., Zhang S. and Lv W. (2005). "Treatment of wastewater from a monosodium glutamate manufacturing plant using successive yeast and activated sludge systems." Process Biochem 40 (7):2483-2488.
Yao S., Ni J., Ma T. and Li C. (2013). "Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp. HA2." Bioresour Technol 139:80-86.
Yu H., Yuan M., Lu W., Yang J., Dai S., Li Q., Yang Z., Dong J., Sun L., Deng Z., Zhang W., Chen M., Ping S., Han Y., Zhan Y., Yan Y., Jin Q. and Lin M. (2011). "Complete genome sequence of the nitrogen-fixing and rhizosphere-associated bacterium Pseudomonas stutzeri strain DSM4166." J Bacteriol 193 (13):3422-3423.
Zawadzka A. M., Crawford R. L. and Paszczynski A. J. (2007). "Pyridine-2,6-bis(thiocarboxylic acid) produced by Pseudomonas stutzeri KC reduces chromium(VI) and precipitates mercury, cadmium, lead and arsenic." Biometals 20 (2):145-158.
Zhang J., Wu P., Hao B. and Yu Z. (2011). "Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001." Bioresour Technol 102 (21):9866-9869.
Zhang Q. L., Liu Y., Ai G. M., Miao L. L., Zheng H. Y. and Liu Z. P. (2012). "The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7." Bioresour Technol 108:35-44.
Zhang S. M., Li W. G., Zhang D. Y., Huang X. F., Qin W. and Gu J. (2013). "Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16." Desalin Water Treat(ahead-of-print):1-6.
Zhang S. M., Li W. G., Zhang D. Y., Huang X. F., W Q. and Q S. C. (2014). "Purification and Characterization of a Low-Temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16." Biomed Environ Sci(27).
Zhang X., Fu L., Deng B., Liang Q., Zheng J., Sun J., Zhu H., Peng L., Wang Y., Wenying S. and Li W. (2013). "Bacillus subtilis SC02 supplementation causes alterations of the microbial diversity in grass carp water." World J Microbiol Biotechnol 29 (9):1645-1653.
Zumft W. G. (2005). "Nitric oxide reductases of prokaryotes with emphasis on the respiratory, heme-copper oxidase type." J Inorg Biochem 99 (1):194-215.
Zumft W. G., Braun C. and Cuypers H. (1994). "Nitric oxide reductase from Pseudomonas stutzeri. Primary structure and gene organization of a novel bacterial cytochrome be complex." Eur J Biochem 219 (1-2):481-490.
蔡妙英,东秀珠(2001).常见细菌系统鉴定手册.北京,科学出版社.
蔡亚君,桂震,李锋,杨小俊(2011).”多功能水处理菌Pseudomonas stutzeri CDNl的筛选分离与性质鉴定.”湖北农业科学50(5)：908-910.
陈会克(1995).”光合细菌及其应用.”河南水产(02)：9-10.
陈营,王福强,邵占涛,王斌(2006).”乳酸菌对牙鲆稚鱼养殖水体和肠道菌群的影响.”海洋水产研究27(3)：37-41.
樊慧俐(2009).斯氏假单胞菌A1501固氮岛中NifA依赖的未知功能基因转录特性研究,中国农业科学院.硕士：77.
范毛毛,夏磊,杨仲明,赵淑坤(2011).”高性能乳酸菌在草鱼养殖中的应用试验.”现代渔业科技产业(3)：6-8.
丰茂武,吴云海,冯仕训,吴云影(2008).”不同氮磷比对藻类生长的影响.”生态环境17(5)：1759-1763.
冯俊荣,陈营,付学军,王爱敏(2005).”微生态制剂对养殖水体水质条件的影响.”海洋湖沼通报(4)：104-108.
付保荣,曹向宇,冷阳,顾继光,马汐平,李法云,杜麟(2008).”光合细菌对水产养殖水质和水生生物的影响.”生态科学27(2)：102-106.
付殿国,徐明(2005).”微生态制剂.”吉林畜牧兽医(4)：13-15.
国家环保局水和废水监测分析方法编委会(2002).水和废水检测分析方法(第四版).北京,中国环境科学出版.
国家环境保护局(1989).中国渔业水质标准GB11607-89.北京,中国标准出版社.
侯树宇,张清敏,多淼,王兰,钟欢,任富海(2004).”微生态复合菌制剂在对虾养殖中的应用研究.”农业环境科学学报23(5)：904-907.
胡金星,汤江武,王新,姚晓红,吴逸飞,葛向阳(2012).”一株反硝化细菌的鉴定、功能基因检测及其反硝化特性.”环境科学与技术(12)：27-32.
解志红,杨毅,平淑珍,陈明,张维,陆伟,徐玉泉,刘洪娟,王国英,Elmerich C.,林敏(2005).”利用酵母双杂交系统研究斯氏假单胞菌固氮调节蛋白NifL和NifA的相互作用.”科学通报50(1)：97-99.
孔庆鑫,李君文(2004).”微生物好氧反硝化脱氮作用研究进展.”环境与健康杂志21(3)：189-191.
孔庆鑫,李君文,王新为,金敏,古长庆(2005).“一种新的好氧反硝化菌筛选方法的建立及新菌株的发现.”应用与环境生物学报11(2)：222-225.
兰亚红(2008).施氏假单胞杆菌的毒死蜱降解酶的基本特性及其制剂的研究,中国农业科学院.
李超波,李文明,杨文华,李海星,刘晓华,曹郁生(2012).“黄曲霉毒素B1降解菌的分离鉴定及其降解特性.”微生物学报52(9)：1129-1136.
李明春,姜恒,侯文强,邢来君(1998,).“酵母菌对重金属离子吸附的研究.”菌物系统(04)：80-86.
李卫芬,傅罗琴,邓斌,陈南南,周绪霞(2011).“1株好氧反硝化菌的分离鉴定及反硝化特性研究.”环境科学32(8)：2403-2408.
李文明,杨文华,李海星,刘晓华,曹郁生(2013).”施氏假单胞菌F4对黄曲霉毒素B1的酶解作用及其降解产物的初步分析.”食品与发酵工业39(2)：11-16.
林娜,郭楚玲,柯林,党志(2010).”富营养化池塘中好氧反硝化菌的分布及脱氮研究.”中国科技论文在线5(5)：369-372,376.
刘慧玲,张战锋,李长玲,黄翔鹄,李瑞伟,林锡雄(2009).”光合细菌对罗非鱼鱼苗养殖水质及抗病力的影响.”渔业现代化36(2)：47-51.
刘万飞,王西亮,赵宇慧,曾瀞瑶,耿佳宁,胡松年(2011).”基于第二代测序技术的细菌基因组与转录组研究策略简介.”微生物学通报38(11)：1705-1714.
刘英霞,常显波,杨启霞(2010).“EM菌对养虾池水质的作用效果.”安徽农业科学38(13)：6891-6892.
马海军,赵振良,穆珂馨(2010).”复合微生态制剂在半封闭混养池塘中的应用.”大连海洋大学学报25(6)：556-559.
马展(2003).城市污水处理系统的菌群分析和nap基因的克隆表达,重庆医科大学
彭小珍,吉宏武,刘唤明,章超桦(2007).”施氏假单胞菌Pseudomonas stutzeri产 卡拉胶酶液体发酵条件优化.”食品研究与开发28(10)：7-11.
彭自然,臧维玲,高杨,江敏,徐桂荣,丁福江(2004).”氨和亚硝酸盐对凡纳滨对虾幼虾的毒性影响.”上海水产大学学报13(3)：274-278.
史德青,赵金生,杨金荣,侯影飞,孔瑛(2004).”施氏假单胞菌对二苯并噻吩的降解.”中国环境科学24(6)：730-733.
史建华,徐琴英,王韩信,李建忠,朱选才(2007).”生石灰在水产养殖中的应用.”水产科技情报34(2)：86-89.
宋蓉(2012).乳酸菌对养殖水体亚硝酸盐控制机理的研究,华中农业大学.
田凤蓉,刘英,吕文洲,呼世斌(2011).”酵母菌产乳化剂能力及其对含油废水降解性能的影响研究.”环境工程学报05(5)：1062-1066.
田功太,刘飞,段登选,杜兴华,张金路,陈述江,张明磊,王慧(2012).“EM菌对海参养殖水体主要污染物净化效果的研究.”山东农业大学学报(自然科学版)43(3)：381-386.
王彬彬,熊丽,郑永良,张志霞,童文羽,刘松,陈奕和,肖文精,刘德立(2008).”甲基对硫磷高效降解菌的分离鉴定及降解酶基因的克隆表达.”环境科学学报28(10)：1969-1975.
王春德,齐翠红,李朝霞(2012).”过氧化钙的净水增氧效果评估.”生命科学研究16(4)：345-349.
王峰慧,智利,魏巍,苏俊峰(2011).”微生态菌剂直投技术原位修复微污染水源水的试验研究.”给水排水37(1)：143-146.
王连峰,蔡祖聪(2004).”水分和温度对旱地红壤硝化活力和反硝化活力的影响.”土壤36(5)：543-546,560.
王瑞(2011).固氮施氏假单胞菌ACC脱氨酶基因acdS的功能鉴定,中国农业科学院.
王莹,周巧红,梁威,徐栋,蔡林林,陶敏,吴振斌(2010).”人工湿地高效好氧反硝化菌的分离鉴定及反硝化特性研究.”农业环境科学学报29(6)：1193-1198.
王有乐,雷兴龙,王亚变,杨勤(2010).”好氧反硝化菌筛选方法的研究进展.”安徽农业科学38(10)：5294-5296,5300.
王志盈,袁林江,彭党聪,刘超翔(2000).”内循环生物流化床硝化过程的选择特性研究.”中国给水排水16(4)：1-4.
韦璐,江敏,余根鼎,李世凯,戴习林,顾德平,胡伟国(2012).”凡纳滨对虾养殖塘叶绿素a与水质因子主成分多元线性回归分析.”中国水产科学19(4)：620-625.
吴鹏霞(2009).异养硝化细菌的分离、施氏假单胞菌YZN-001的鉴定及特性研究,华中农业大学.硕士：55.
谢航,邱宏端,林娟,陈朝洋(2005).”假丝酵母菌降解养殖水体氨氮的特性研究.”农业工程学报21(8)：142-145.
辛玉峰,曲晓华,袁梦冬,荆延德(2011).”一株异养硝化-反硝化不动杆菌的分离鉴定及脱氮活性.”微生物学报51(12)：1646-1654.
邢殿楼,霍堂斌,吴会民,刘靖,刘长发,雷衍之(2006).”总磷、总氮联合消化的测定方法.”大连水产学院学报21(3)：219-225.
熊小京,李博,林光辉,郑天凌,彭义亮(2011).”包埋固定化EM菌的氨氮去除特性研究.”环境卫生工程19(2)：39-41,44.
徐爱玲,张帅,张燕飞,历丽,杨宇,夏金兰(2008).”积累PHB菌种隐藏嗜酸菌DX1-1的诱变改良.”微生物学通报35(10)：1516-1521.
燕永亮(2005).斯氏假单胞菌(Pseudomonas stutzeri)A1501的基因组学研究,中国农业大学.
杨俊忠,倪砚,许尚营,徐可瀚,刘义,曾丽霞,刘德立(2010).”一株高效好氧反硝化菌的分离及特性.”微生物学通报37(11)：1594-1599.
杨小龙(2012).复合菌剂的研制及其对水产养殖污水的净化作用,南昌大学.
杨小龙,李文明,陈燕,曹郁生(2011).”一株好氧反硝化菌的分离鉴定及其除氮特性.”微生物学报51(8)：1062-1070.
杨益民(2011).叶绿素a荧光检测仪的设计与开发,浙江大学.
易力,汪洋,陈万光,张耀武(2011).”固定化浓缩光合细菌对养殖水环境的影响.”贵州农业科学39(6)：152-154.
殷芳芳,王淑莹,昂雪野,侯红勋,彭永臻,王伟(2009).”碳源类型对低温条件下生物反硝化的影响.”环境科学30(1)：108-113.
尹文林,沈锦玉,潘晓艺,姚嘉赞,徐洋,郝贵杰(2010).”复合硝化菌制剂对水质改良的应用效果.”水产养殖31(9)：12-17.
尹艳娥,沈新强,晁敏,全为民,王云龙(2009).”反硝化技术对模拟养殖池塘修复的研究.”农业环境科学学报28(8)：1727-1732.
余日清,贺锡勤(1992).”低pH对草鱼血液酸碱平衡的影响.”环境科学学报12(1)：112-118.
余雄奎(2008).固定化藻菌生物修复富营养化水体的模拟研究,武汉科技大学.
詹金星,支崇远,夏品华,马建荣,李海珍(2011).”水生植物净化污水的机理及研究进展.”西南农业学报24(1)：352-355.
张峰峰,谢凤行,赵玉洁,周可,李亚玲(2013).”一株反硝化细菌的分离鉴定及脱氮能力.”环境工程学报7(11)：4415-4420.
张凯,雷梦婕,胡国元,袁军,杨洋,章建国(2011).”好氧反硝化细菌WIT-1的分离鉴定及其脱氨氮特性.”武汉工程大学学报33(11)：14-18.
张秋磊(2008).固氮斯氏假单胞菌A1501鞭毛调控基fleQ的功能研究,中国农业科学院.
张小玲,袁科平,耿康,张玲,杨丽(2011).”好氧反硝化菌对水质和鱼体饲喂效果的影响研究.”水生态学杂志32(3)：114-119.
郑佳佳,张小平,胡彩虹,李卫芬(2013).”斯氏假单胞菌对草鱼养殖水体水质的调控作用.”渔业现代化(3)：5-9.
中华人民共和国农业部(2013).饲料添加剂品种目录.
周淑云(2006).毒死蜱高效降解菌的分离鉴定及其生物学特性研究,西南大学.
周鑫,王素英,宋霖霞(2010).”海水养殖含氮废水处理的复合微生态制剂研制.”微生物学通报37(4)：543-546.
朱红瑜(2007).屠宰废水生物处理低温菌种的筛选,吉林农业大学.
朱亮,汪岁羽,朱雪诞,邵孝侯,刘德有(2002).“EM菌富集培养及降解污水试验研究.”河海大学学报(自然科学版)30(2)：6-8.
朱明瑞,曹广斌,蒋树义,韩世成(2007).”工厂化水产养殖水体的pH值在线自动控制系统.”水产学报31(3)：335-342.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |