红莲型水稻atp6-orfH79基因的转录加工及其育性恢复相关基因的功能研究
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摘要
细胞质雄性不育水稻根据遗传和细胞学特征分为野败(WA)、红莲(HL)和包台(BT)三大类型,其中红莲型水稻是武汉大学在上世纪70年代选育的配子体细胞质雄性不育类型,目前在生产上得到了大面积推广与应用。本论文对红莲型水稻细胞质雄性不育和育性恢复的分子机理进行了一些探索,获得以下研究结果：
1.采用Northern blot、引物延伸、CR-RT-PCR和RACE的方法,我们系统地对红莲型CMS水稻不育系及其杂种F1代线粒体中不育相关基因的转录和转录后水平进行了细致的分析。并基于这些分子水平的结果将CMS红莲型和包台型水稻做了全面的比较分析。发现两者的不育相关区域atp6-orfH79和atp6-orf79,在不育系中的转录模式和杂种中恢复基因的加工位点等方面存在一些明显的差异：(1)对HL型CMS水稻的不育系不育相关区域的分析表明atp6-orfH79的基因间区域存在转录启动子。转录起始位点位于1117和1124,转录本长度分别为483bp和476bp。密度梯度离心实验证实这些新的转录本可能与核糖体共分离,推测其具有翻译能力。(2)对HL型CMS水稻杂种F1代(9311为父本)的分析表明9311中的恢复基因在atp6-orfH79共转录本上的加工位点有三个,其中一个位于atp6上游-11--13位,另外两个位于基因间区域的1132和1170位。(3)对33个含有atp6-orfH79同源片段的水稻基因型的聚类分析表明(基于非编码的基因间区序列比对),atp6-orfH79和atp6-orfH79分属两大类。
2.为了深入研究红莲型水稻不育和育性恢复的分子机理,我们构建了红莲型水稻杂种F1的幼穗细菌双杂交系统文库。用Trizol试剂提取红莲型水稻杂种F1幼穗的总RNA,分离纯化mRNA后反转录并合成双链cDNA。经sephrose CL-2B凝胶过滤柱层析后,收集大于400 bp以上的双链cDNA片段,将其连接到pTRG载体上,转化到XL1-Blue MRF'Kan感受态细胞后构建成红莲水稻幼穗细菌双杂交文库。经检测计算该原始文库容量为1.03×106pfu/mL,对随机挑取的100个克隆进行PCR鉴定,显示重组率接近100%,而且大于800 bp的插入片段达到97%。扩增后文库的容量达到1.05×1010pfu/mL。
3.一些研究报道认为CMS的恢复基因可能是以复合体的形式发挥加工转录本的功能。为了搞清楚红莲型CMS恢复基因Rf5的恢复机理,我们对细菌双杂交文库淘洗的RF5可能的互作蛋白之一的GRP蛋白进行了详细的分析。(1)原核表达并纯化了RF5和GRP重组蛋白,并分别用圆二色谱对其二级结构进行分析,证明表达的重组蛋白具有典型的PPR和RRM基序所具有的二级结构特征。(2)凝胶阻滞和SPR(表面等离子共振)实验证明GRP蛋白能够与H-atp6的基因间区域的RNA相互作用而且分析了GRP蛋白与靶标RNA的结合常数等参数。酵母三杂交实验进一步将GRP与RNA的相互作用确定在两个较小的区段。
The CMS rice generally could be categorized into three types, namely, WA-type, Honglian(HL)-type and BT-type. The HL-type CMS rice was discovered in the last century's 70s by Wuhan University. Currently, it had been promoted and applied to large cultivated area of hybrid rice. The molecular mechanisms for CMS and fertility restoration of Honglian CMS system were performed in this paper. The results were shown as follow.
1. With Northern blot, primer extension, CR-RT-PCR and RACE methods, a detailed analysis was made about CMS associated gene of HL-type rice sterility line Yuetai A(YTA) and hybrid F1 Honglian you-6(HL-6) on transcriptional and post-transcriptional level. Based on this, a comparative study between HL-type and BT-type at the molecular level was done. From the results, some similarities were found. However, there did exist some mentionable differences in transcription mode of CMS associated gene in sterility line and cleavage sites functioned by restorer gene in hybrid F1 between BT-type and HL-type CMS rice. Main results are as follows: (1) Transcript initiation sites 1117 and 1124 in the intergenic region of H-atp6 were found, the length of novel transcripts were 483 and 476 bp. Density gradient centrifugation assay verified that the 483 and 476 bp of transcipts would be associated with polysome, which implied that the novel transcripts possessed the ability of translation. (2) There might be 3 of cleavage sites on the atp6-orfH79 transcript by the restorer gene(s) in 9311. one was at the-11--13 bp upstream of atp6, the other two were at 1132 and 1170 of the intergenic region of atp6-orfH79 transcript.(3) a phylogenetic research was carried through based on DNA sequences of intergenic region of CMS associated gene among 33 rice accessions with orfH79, the results showed that the B-atp6 and H-atp6 respectively belonged to different subgroups.
2. In order to study the molecular mechanism of sterility and fertility restoration in Honglian CMS rice, the total RNA was extracted from the Young Panicle of F1 hybrid rice by Trizol reagent. The mRNA was purified from total RNA by Poly-A Tract mRNA Isolation Kit and the double-stranded(ds) cDNA was synthesized after reverse transcription. The fragments longer than 400 bp in length were fractionated by Sephrose CL-2B Column, and then ligated into the pTRG vertor. The ligation product was transformed into XL 1-Blue MRF'Kan Library Pack Competent Cells to construct the bacterial Two-Hybrid system library. Analysis showed that the primary library contained 1.03×106 pfu/mL, and approximately 100% of the clones were positive recombinants and about 97% of the cDNA fragments inserted were longer than 800 bp in length. The capacity of the amplified library was 2.14×1012 pfu/mL.
3. Many researchers thought that the restorer gene(s) play their function of processing transcript in the form of complex. For clarifying the mechanism of fertility restoration by restorer gene Rf5 in CMS-HL rice, we conducted a detailed analysis on the RF5 and GRP protein which was a candidate interactor with RF5. The main findings were as follows:(1) Recombinant protein of RF5 and GRP were expressed and purified in E. coli. The secondary structure of these two proteins were analyed by circular dichroism and the results showed that the secondary structures predicted by CD matched the characteristic of PPR and RRM motif. (2) The qualitative and quantitative interaction between GRP and the intergenic RNA of H-atp6 in vitro was acquired by gel shift and SPR assays. Furthermore, the more precise bind region in two small sections was identified by yeast three hybrid assay.
1.采用Northern blot、引物延伸、CR-RT-PCR和RACE的方法,我们系统地对红莲型CMS水稻不育系及其杂种F1代线粒体中不育相关基因的转录和转录后水平进行了细致的分析。并基于这些分子水平的结果将CMS红莲型和包台型水稻做了全面的比较分析。发现两者的不育相关区域atp6-orfH79和atp6-orf79,在不育系中的转录模式和杂种中恢复基因的加工位点等方面存在一些明显的差异：(1)对HL型CMS水稻的不育系不育相关区域的分析表明atp6-orfH79的基因间区域存在转录启动子。转录起始位点位于1117和1124,转录本长度分别为483bp和476bp。密度梯度离心实验证实这些新的转录本可能与核糖体共分离,推测其具有翻译能力。(2)对HL型CMS水稻杂种F1代(9311为父本)的分析表明9311中的恢复基因在atp6-orfH79共转录本上的加工位点有三个,其中一个位于atp6上游-11--13位,另外两个位于基因间区域的1132和1170位。(3)对33个含有atp6-orfH79同源片段的水稻基因型的聚类分析表明(基于非编码的基因间区序列比对),atp6-orfH79和atp6-orfH79分属两大类。
2.为了深入研究红莲型水稻不育和育性恢复的分子机理,我们构建了红莲型水稻杂种F1的幼穗细菌双杂交系统文库。用Trizol试剂提取红莲型水稻杂种F1幼穗的总RNA,分离纯化mRNA后反转录并合成双链cDNA。经sephrose CL-2B凝胶过滤柱层析后,收集大于400 bp以上的双链cDNA片段,将其连接到pTRG载体上,转化到XL1-Blue MRF'Kan感受态细胞后构建成红莲水稻幼穗细菌双杂交文库。经检测计算该原始文库容量为1.03×106pfu/mL,对随机挑取的100个克隆进行PCR鉴定,显示重组率接近100%,而且大于800 bp的插入片段达到97%。扩增后文库的容量达到1.05×1010pfu/mL。
3.一些研究报道认为CMS的恢复基因可能是以复合体的形式发挥加工转录本的功能。为了搞清楚红莲型CMS恢复基因Rf5的恢复机理,我们对细菌双杂交文库淘洗的RF5可能的互作蛋白之一的GRP蛋白进行了详细的分析。(1)原核表达并纯化了RF5和GRP重组蛋白,并分别用圆二色谱对其二级结构进行分析,证明表达的重组蛋白具有典型的PPR和RRM基序所具有的二级结构特征。(2)凝胶阻滞和SPR(表面等离子共振)实验证明GRP蛋白能够与H-atp6的基因间区域的RNA相互作用而且分析了GRP蛋白与靶标RNA的结合常数等参数。酵母三杂交实验进一步将GRP与RNA的相互作用确定在两个较小的区段。
The CMS rice generally could be categorized into three types, namely, WA-type, Honglian(HL)-type and BT-type. The HL-type CMS rice was discovered in the last century's 70s by Wuhan University. Currently, it had been promoted and applied to large cultivated area of hybrid rice. The molecular mechanisms for CMS and fertility restoration of Honglian CMS system were performed in this paper. The results were shown as follow.
1. With Northern blot, primer extension, CR-RT-PCR and RACE methods, a detailed analysis was made about CMS associated gene of HL-type rice sterility line Yuetai A(YTA) and hybrid F1 Honglian you-6(HL-6) on transcriptional and post-transcriptional level. Based on this, a comparative study between HL-type and BT-type at the molecular level was done. From the results, some similarities were found. However, there did exist some mentionable differences in transcription mode of CMS associated gene in sterility line and cleavage sites functioned by restorer gene in hybrid F1 between BT-type and HL-type CMS rice. Main results are as follows: (1) Transcript initiation sites 1117 and 1124 in the intergenic region of H-atp6 were found, the length of novel transcripts were 483 and 476 bp. Density gradient centrifugation assay verified that the 483 and 476 bp of transcipts would be associated with polysome, which implied that the novel transcripts possessed the ability of translation. (2) There might be 3 of cleavage sites on the atp6-orfH79 transcript by the restorer gene(s) in 9311. one was at the-11--13 bp upstream of atp6, the other two were at 1132 and 1170 of the intergenic region of atp6-orfH79 transcript.(3) a phylogenetic research was carried through based on DNA sequences of intergenic region of CMS associated gene among 33 rice accessions with orfH79, the results showed that the B-atp6 and H-atp6 respectively belonged to different subgroups.
2. In order to study the molecular mechanism of sterility and fertility restoration in Honglian CMS rice, the total RNA was extracted from the Young Panicle of F1 hybrid rice by Trizol reagent. The mRNA was purified from total RNA by Poly-A Tract mRNA Isolation Kit and the double-stranded(ds) cDNA was synthesized after reverse transcription. The fragments longer than 400 bp in length were fractionated by Sephrose CL-2B Column, and then ligated into the pTRG vertor. The ligation product was transformed into XL 1-Blue MRF'Kan Library Pack Competent Cells to construct the bacterial Two-Hybrid system library. Analysis showed that the primary library contained 1.03×106 pfu/mL, and approximately 100% of the clones were positive recombinants and about 97% of the cDNA fragments inserted were longer than 800 bp in length. The capacity of the amplified library was 2.14×1012 pfu/mL.
3. Many researchers thought that the restorer gene(s) play their function of processing transcript in the form of complex. For clarifying the mechanism of fertility restoration by restorer gene Rf5 in CMS-HL rice, we conducted a detailed analysis on the RF5 and GRP protein which was a candidate interactor with RF5. The main findings were as follows:(1) Recombinant protein of RF5 and GRP were expressed and purified in E. coli. The secondary structure of these two proteins were analyed by circular dichroism and the results showed that the secondary structures predicted by CD matched the characteristic of PPR and RRM motif. (2) The qualitative and quantitative interaction between GRP and the intergenic RNA of H-atp6 in vitro was acquired by gel shift and SPR assays. Furthermore, the more precise bind region in two small sections was identified by yeast three hybrid assay.
引文
Akagi, H., Sakamoto, M., Shinjyo, C., Shimada, H., and Fujimura, T. (1994). A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility. Curr Genet 25,52-58.
Andeol Falcon de, L., Luke, H., Nicolas, L.T., Etienne, D., Claire, L., Murray, B., Millar, A.H., and Ian, S. (2008). The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana. The Plant Journal 56,157-168.
Anne-Laure, C.-B., Maricela, R.-V., Arturo, G.-G, Charles, A., Maria de la Luz, G.-N., Araceli, C., Etienne, D., Luis Felipe, J., Claire, L., Ian, S., and Patricia, L. (2008). CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts. The Plant Journal 56, 590-602.
Backert, S., Lynn Nielsen, B., and Borner, T. (1997). The mystery of the rings: structure and replication of mitochondrial genomes from higher plants. Trends in Plant Science 2,477-483.
Balk,J., and Leaver, C.J. (2001). The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release. Plant Cell 13,1803-1818.
Bergman, P., Edqvist, J., Farbos, I., and Glimelius, K. (2000). Male-sterile tobacco displays abnormal mitochondrial atpl transcript accumulation and reduced floral ATP/ADP ratio. Plant Molecular Biology 42,531-544.
Bonhomme, S., Budar, F., Ferault, M., and Pelletier, G. (1991). A 2.5 kb NcoI fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male-sterility in Brassica cybrids. Curr. Genet.19,121-127.
Bras, M., Queenan, B., and Susin, S. (2005). Programmed cell death via mitochondria:Different modes of dying. Biochemistry (Moscow) 70,231-239.
Brown, G.G., Formanova, N., Jin, H., Wargachuk, R., Dendy, C., Patil, P., Laforest, M., Zhang, J., Cheung, W.Y., and Landry, B.S. (2003). The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35,262-272.
Burger, G., Gray, M.W., and Franz Lang, B. (2003). Mitochondrial genomes: anything goes. Trends in Genetics 19,709-716.
Chase, C.D. (2007). Cytoplasmic male sterility:a window to the world of plant mitochondrial-nuclear interactions. Trends Genet 23,81-90.
Chinnery, P.F. (2003). Searching for nuclear-mitochondrial genes. Trends in Genetics 19,60-62.
Clery, A., Blatter, M., and Allain, F.H. (2008). RNA recognition motifs:boring? Not quite. Curr Opin Struct Biol 18,290-298.
Daniell, H., Kumar, S., and Dufourmantel, N. (2005). Breakthrough in chloroplast genetic engineering of agronomically important crops 23,238-245.
Delannoy, E., Stanley, W.A., Bond, C.S., and Small, I.D. (2007). Pentatricopeptide repeat (PPR) proteins as sequence-specificity factors in post-transcriptional processes in organelles. Biochemical Society Transactions 35,1643-1647.
Desloire, S., Gherbi, H., Laloui, W., Marhadour, S., Clouet, V., Cattolico, L., Falentin, C., Giancola, S., Renard, M., Budar, F., Small, I., Caboche, M., Delourme, R., and Bendahmane, A. (2003). Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family. EMBO Rep 4,588-594.
Ding, Y.-H., Liu, N.-Y., Tang, Z.-S., Liu, J., and Yang, W.-C. (2006). Arabidopsis GLUTAMINE-RICH PROTEIN23 Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase Ⅱ Subunit Ⅲ. Plant Cell 18,815-830.
Duan, S.-H., Li, S.-Q., Li, Y.-S., Xiong, Y., and Zhu, Y.-G. (2007). Distribution and SNPs of the rice CMS-related gene in AA-genome of Oryza species. Yichuan 29,455-461.
Duroc, Y., Hiard, S., Vrielynck, N., Ragu, S., and Budar, F. (2009). The Ogura sterility-inducing protein forms a large complex without interfering with the oxidative phosphorylation components in rapeseed mitochondria. Plant Mol.Biol.70,123-137.
Duroc, Y., Gaillard, C., Hiard, S., Defrance, M.C., Pelletier, G., and Budar, F. (2005). Biochemical and functional characterization of ORF138, a mitochondrial protein responsible for Ogura cytoplasmic male sterility in Brassiceae. Biochimie 87,1089-1100.
Eubel, H., Heazlewood, J.L., and Millar, A.H. (2007). Isolation and subfractionation of plant mitochondria for proteomic analysis. Methods Mol Biol 355,49-62.
Fey, J., and Marechal-Drouard, L. (1999). Compilation and Analysis of Plant Mitochondrial Promoter Sequences:An Illustration of a Divergent Evolution between Monocot and Dicot Mitochondria. Biochemical and Biophysical Research Communications 256,409-414.
Fields, S., and Song, O.-k. (1989). A novel genetic system to detect protein-protein interactions. Nature 340,245-246.
Fisk, D.G., Walker, M.B., and Barkan, A. (1999). Molecular cloning of the maize gene crpl reveals similarity between regulators of mitochondrial and chloroplast gene expression. Embo J 18,2621-2630.
Forner, J., Weber, B., Thuss, S., Wildum, S., and Binder, S. (2007). Mapping of mitochondrial mRNA termini in Arabidopsis thaliana:t-elements contribute to 5'and 3'end formation. Nucl. Acids Res.35,3676-3692.
Fujii, S., and Toriyama, K. (2008). Genome Barriers between Nuclei and Mitochondria Exemplified by Cytoplasmic Male Sterility. Plant Cell Physiol. 49,1484-1494.
Fujii, S., Kazama, T., and Toriyama, K. (2008). Molecular Studies on Cytoplasmic Male Sterility-associated Genes and Restorer Genes in Rice. In Rice Biology in the Genomics Era.Biotechnology in Agriculture and Forestry (Berlin Heidelberg:Springer.
Gieg, P., and Brennicke, A. (2001). From gene to protein in higher plant mitochondria. Life Sciences 324,209-217.
Gillman, J.D., Bentolila, S., and Hanson, M.R. (2007). The petunia restorer of fertility protein is part of a large mitochondrial complex that interacts with transcripts of the CMS-associated locus. Plant J.49,217-227.
Gonzalez-Melendi, P., Uyttewaal, M., Morcillo, C.N., Mora, J.R.H., Fajardo, S., Budar, F., and Lucas, M.M. (2008). A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). Journal of Experimental Botany 59,827-838.
Handa, H., Gualberto, J.M., and Grienenberger, J.-M. (1995). Characterization of the mitochondrial orfB gene and its derivative, orf224, a chimeric open reading frame specific to one mitochondrial genome of the "Polima" male-sterile cytoplasm in rapeseed (Brassica napus L.). Curr. Genet.28, 546-552.
Hanson, M.R., and Bentolila, S. (2004). Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development. Plant Cell 16, S154-169.
Hanson, M.R., Wilson, R.K., Bentolila, S., Kohler, R.H., and Chen, H.C. (1999). Mitochondrial gene organization and expression in petunia male fertile and sterile plants. J Hered 90,362-368.
Hattori, M., Miyake, H., and Sugita, M. (2007). A pentatricopeptide repeat protein is required for RNA processing of clpP pre-mRNA in moss chloroplasts. Journal of Biological Chemistry 282,10773-10782.
Hoang, V.T., Daryl, R.P., Lynn, C.S., Reggie, A.S., Figuhr, R.M., Bin, Y., and Keith, F.S. (1996). Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum. The Plant Journal 10,123-133.
Hook, B., Bernstein, D., Zhang, B., and Wickens, M. (2005). RNA-protein interactions in the yeast three-hybrid system:Affinity, sensitivity, and enhanced library screening. RNA 11,227-233.
Horn, R., Kohler, R.H., and Zetsche, K. (1991). A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol.Biol.17, 29-36.
Hu, J.C., Kornacker, M.G, and Hochschild, A. (2000). Escherichia coli one-and two-hybrid systems for the analysis and identification of protein-protein interactions. Methods 20,80-94.
Ikeda, T.M., and Gray, M.W. (1999). Characterization of a DNA-Binding Protein Implicated in Transcription in Wheat Mitochondria. Mol. Cell. Biol.19, 8113-8122.
Iwabuchi, M., Kyozuka, J., and Shimamoto, K. (1993). Processing followed by complete editing of an altered mitochondrial atp6 RNA restores fertility of cytoplasmic male sterile rice. Embo J 12,1437-1446.
Iwabuchi, M., Koizuka, N., Fujimoto, H., Sakai, T., and Imamura, J. (1999). Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orfl38. Plant Mol.Biol.39,183-188.
Jenny, C., Ulf, L., Jens, S., Rita, T., Frank, W., Elliot, M.M., and Kristina, G. (2007). Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterileBrassica napus flowers. The Plant Journal 49,452-462.
Jobling, M.G, and Holmes, R.K. (2000). Identification of motifs in cholera toxin Al polypeptide that are required for its interaction with human ADP-ribosylation factor 6 in a bacterial two-hybrid system. Proc Natl Acad Sci U S A 97, 14662-14667.
Joung, J.K., Ramm, E.I., and Pabo, C.O. (2000). A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97,7382-7387.
Kadowaki, K., Suzuki, T., and Kazama, S. (1990). A chimeric gene containing the 5'portion of atp6 is associated with cytoplasmic male-sterility of rice. Mol Gen Genet 224,10-16.
Kang, S., Han, J.S., Park, J.H., Skarstad, K., and Hwang, D.S. (2003). SeqA protein stimulates the relaxing and decatenating activities of topoisomerase Ⅳ. J Biol Chem 278,48779-48785.
Kaul, M.L.H. (1988). Male Sterility in Higher Plants. Male Sterility in Higher Plants 4.
Kazama, T., Nakamura, T., Watanabe, M., Sugita, M., and Toriyama, K. (2008). Suppression mechanism of mitochondrial ORF79 accumulation by Rfl protein in BT-type cytoplasmic male sterile rice. The Plant Journal 55,619-628.
Kim, D., Kang, J., and Kim, B.-D. (2007). Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.). Plant Mol.Biol.63,519-532.
Koizuka, N., Imai, R., Fujimoto, H., Hayakawa, T., Kimura, Y., Kohno-Murase, J., Sakai, T., Kawasaki, S., and Imamura, J. (2003). Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J.34, 407-415.
Kotera, E., Tasaka, M., and Shikanai, T. (2005). A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts. Nature 433,326-330.
Kubo, T., and Newton, K.J. (2008). Angiosperm mitochondrial genomes and mutations. Mitochondrion 8,5-14.
Kuhn, J., and Binder, S. (2002). RT-PCR analysis of 5'to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria. Nucl. Acids Res.30,439-446.
Kuhn, J., Tengler, U., and Binder, S. (2001). Transcript Lifetime Is Balanced between Stabilizing Stem-Loop Structures and Degradation-Promoting Polyadenylation in Plant Mitochondria. Mol. Cell. Biol.21,731-742.
Kuhn, K., Weihe, A., and Borner, T. (2005). Multiple promoters are a common feature of mitochondrial genes in Arabidopsis. Nucl. Acids Res.33,337-346.
Kumar, S., Nei, M., Dudley, J., and Tamura, K. (2008). MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9,299-306.
L'Homme, Y., Stahl, R.J., Li, X.-Q., Hameed, A., and Brown, G.G. (1997). Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene. Curr. Genet.31,325-335.
Landgren, M., Zetterstrand, M., Sundberg, E., and Glimelius, K. (1996). Alloplasmic male-sterile Brassica lines containing B. tournefortii mitochondria express an ORF 3 of the atp6 gene and a 32 kDa protein. Plant Mol.Biol.32,879-890.
Lee, H., Kang, S., Bae, S.H., Choi, B.S., and Hwang, D.S. (2001). SeqA protein aggregation is necessary for SeqA function. J Biol Chem 276,34600-34606.
Li, S.Q., Yang, D.C., and Zhu, Y.G. (2007). Characterization and use of male sterility in hybrid rice breeding. J. Integr. Plant Biol.49,791-804.
Li, S.Q., Wan, C.X., Kong, J., Zhang, Z.J., Li, Y.S., and Zhu, Y.G. (2004). Programmed cell death during microgenesis in a Honglian CMS line of rice is correlated with oxidative stress in mitochondria. Funct. Plant Biol.31, 369-376.
Linke, B., and Borner, T. (2005). Mitochondrial effects on flower and pollen development. Mitochondrion 5,389-402.
Liu, F., Cui, X., Horner, H.T., Weiner, H., and Schnable, P.S. (2001). Mitochondrial Aldehyde Dehydrogenase Activity Is Required for Male Fertility in Maize. Plant Cell 13,1063-1078.
Lopian, L., Nussbaum-Shochat, A., O'Day-Kerstein, K., Wright, A., and Amster-Choder, O. (2003). The BglF sensor recruits the BglG transcription regulator to the membrane and releases it on stimulation. Proc Natl Acad Sci U SA100,7099-7104.
Lurin, C., Andres, C., Aubourg, S., Bellaoui, M., Bitton, F., Bruyere, C., Caboche, M., Debast, C., Gualberto, J., Hoffmann, B., Lecharny, A., Le Ret, M., Martin-Magniette, M.-L., Mireau, H., Peeters, N., Renou, J.-P., Szurek, B., Taconnat, L., and Small, I. (2004). Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis. Plant Cell 16,2089-2103.
Mackenzie, S.A., and Chase, C.D. (1990). Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean. Plant Cell 2,905-912.
Manchekar, M., Scissum-Gunn, K., Song, D., Khazi, F., McLean, S.L., and Nielsen, B.L. (2006). DNA Recombination Activity in Soybean Mitochondria. Journal of Molecular Biology 356,288-299.
Manthey, G.M., and McEwen, J.E. (1995). The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae. Embo J14,4031-4043.
Maris, C., Dominguez, C., and Allain, F.H. (2005). The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression. FEBS J 272,2118-2131.
Meierhoff, K., Felder, S., Nakamura, T., Bechtold, N., and Schuster, G. (2003). HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs. Plant Cell 15,1480-1495.
Nakamura, T., and Sugita, M. (2008). A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribonuclease activity. FEBS Lett.582,4163-4168.
O'Toole, N., Hattori, M., Andres, C., Iida, K., Lurin, C., Schmitz-Linneweber, C., Sugita, M., and Small, I. (2008). On the expansion of the pentatricopeptide repeat gene family in plants. Mol. Biol. Evol.25,1120-1128.
Okuda, K., Nakamura, T., Sugita, M., Shimizu, T., and Shikanai, T. (2006). A Pentatricopeptide Repeat Protein Is a Site Recognition Factor in Chloroplast RNA Editing. J. Biol. Chem.281,37661-37667.
Okuda, K., Myouga, F., Motohashi, R., Shinozaki, K., and Shikanai, T. (2007). Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing. Proc. Natl. Acad. Sci. U. S. A.104,8178-8183.
Reichert, A.S., and Neupert, W. (2004). Mitochondriomics or what makes us breathe. Trends in Genetics 20,555-562.
Rossi, F., Charlton, C.A., and Blau, H.M. (1997). Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation. Proc Natl Acad Sci U S A 94,8405-8410.
RP, W., AE, F., DR, P., and BG, G. (1987). urfl3-T of T cytoplasm maize mitochondria encodes a 13kD polypeptide. Plant Mol. Biol.9,121-126.
Sabar, M., Gagliardi, D., Balk, J., and Leaver, C.J. (2003). ORFB is a subunit of F1FO-ATP synthase:insight into the basis of cytoplasmic male sterility in sunflower. EMBO Rep.4,381-386.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning:A Laboratory Manual,2nd edn.
Schmitz-Linneweber, C., and Small, I. (2008). Pentatricopeptide repeat proteins:a socket set for organelle gene expression. Trends in Plant Science 13,663-670.
Schmitz-Linneweber, C., Williams-Carrier, R., and Barkan, A. (2005a). RNA Immunoprecipitation and Microarray Analysis Show a Chloroplast Pentatricopeptide Repeat Protein to Be Associated with the 5'Region of mRNAs Whose Translation It Activates. Plant Cell 17,2791-2804.
Schmitz-Linneweber, C., Williams-Carrier, R., and Barkan, A. (2005b). RNA immunoprecipitation and microarray analysis show a chloroplast pentatricopeptide repeat protein to be associated with the 5'region of mRNAs whose translation it activates. Plant Cell 17,2791-2804.
Schmitz-Linneweber, C., Williams-Carrier, R.E., Williams-Voelker, P.M., Kroeger, T.S., Vichas, A., and Barkan, A. (2006). A Pentatricopeptide Repeat Protein Facilitates the trans-Splicing of the Maize Chloroplast rpsl2 Pre-mRNA. Plant Cell 18,2650-2663.
Segui-Simarro, J.M., Coronado, M.J., and Staehelin, L.A. (2008). The Mitochondrial Cycle of Arabidopsis Shoot Apical Meristem and Leaf Primordium Meristematic Cells Is Defined by a Perinuclear Tentaculate/Cage-Like Mitochondrion. Plant Physiol.148,1380-1393.
Shinjyo, C. (1969). Cytoplasmic genetic male sterility in cultivated rice, Oryza sativa L.. Japanese Journal of Genetics 44,149-156.
Singh, M., and Brown, G.G. (1991). Suppression of Cytoplasmic Male Sterility by Nuclear Genes Alters Expression of a Novel Mitochondrial Gene Region. Plant Cell 3,1349-1362.
Small, I.D., and Peeters, N. (2000). The PPR motif-a TPR-related motif prevalent in plant organellar proteins. Trends Biochem.Sci.25,45-47.
Song, J., and Hedgcoth, C. (1994). A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat. Plant Molecular Biology 26, 535-539.
Tavares-Carreon, F., Camacho-Villasana, Y., Zamudio-Ochoa, A., Shingu-Vazquez, M., Torres-Larios, A., and Perez-Martinez, X. (2008). The pentatricopeptide repeats present in pet309 are necessary for translation but not for stability of the mitochondrial COX1 mRNA in yeast. Journal of Biological Chemistry 283,1472-1479.
Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res.22,4673-4680.
Touzet, P. (2002). Is rf2 a restorer gene of CMS-T in maize? Trends Plant Sci 7,434; author reply 434.
Uyttewaal, M., Mireau, H., Rurek, M., Hammani, K., Arnal, N., Quadrado, M., and Gieg, P. (2008). PPR336 is Associated with Polysomes in Plant Mitochondria. Journal of Molecular Biology 375,626-636.
Wang, Z., Zou, Y., Li, X., Zhang, Q., Chen, L., Wu, H., Su, D., Chen, Y., Guo, J., Luo, D., Long, Y., Zhong, Y., and Liu, Y.-G. (2006). Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing. Plant Cell 18,676-687.
Wen, L., Ruesch, K.L., Ortega, V.M., Kamps, T.L., Gabay-Laughnan, S., and Chase, C.D. (2003). A nuclear restorer-of-fertility mutation disrupts accumulation of mitochondrial ATP synthase subunit alpha in developing pollen of S male-sterile maize. Genetics 165,771-779.
Williams-Carrier, R., Kroeger, T., and Barkan, A. (2008). Sequence-specific binding of a chloroplast pentatricopeptide repeat protein to its native group II intron ligand. RNA14,1930-1941.
Wintz, H., Chen, H.C., Sutton, C.A., Conley, C.A., Cobb, A., Ruth, D., and Hanson, M.R. (1995). Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco. Plant Mol Biol 28,83-92.
Wise, R.P., Bronson, C.R., Schnable, P.S., and Horner, H.T. (1999). The genetics, pathology, and molecular biology of T-cytoplasm male sterility in maize. In Advances in Agronomy, Vol 65 (San Diego:Academic Press Inc), pp.79-130.
Yamamoto, M.P., Shinada, H., Onodera, Y., Komaki, C., Mikami, T., and Kubo, T. (2008). A male sterility-associated mitochondrial protein in wild beets causes pollen disruption in transgenic plants. Plant Journal 54,1027-1036.
Yan, B., and Pring, D.R. (1997). Transcriptional initiation sites in sorghum mitochondrial DNA indicate conserved and variable features. Curr. Genet.32, 287-295.
Yi, P., Wang, L., Sun, Q.P., and Zhu, Y.G. (2002). Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice. Chin. Sci. Bull.47,744-747.
Young, E.G, and Hanson, M.R. (1987). A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50,41-49.
Zhang, Y. (2008). I-TASSER server for protein 3D structure prediction. Bmc Bioinformatics 9,40.
Zhu, Y. (2000). Biology of rice male sterility. (wuhan:Wuhan University Press).
Zhu, Y., Saraike, T., Yamamoto, Y, Hagita, H., Takumi, S., and Murai, K. (2008). orf260(cra), a Novel Mitochondrial Gene, is Associated with the Homeotic Transformation of Stamens into Pistil-Like Structures (Pistillody) in Alloplasmic Wheat. Plant and Cell Physiology 49,1723-1733.
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucl. Acids Res.31,3406-3415.
李绍清.(2004).水稻红莲型细胞质雄性不育与育性恢复的分子机理.武汉大学博士学位论文(武汉：武汉大学).
胡朝凤,彭晓珏,周杨永,谭艳平,李绍清,朱英国.(2008).红莲型水稻杂种F1花药噬菌体展示文库的构建.遗传30,771-775.
彭晓珏.(2008).水稻红莲型细胞质雄性不育基因orfH79的功能鉴定及其分子机理的初步研究.武汉大学博士学位论文(武汉：武汉大学).
张弛,王梁华,罗以勤,张兴群,高云,史薇,包晨颖,球谊,焦炳华.(2003).细菌双杂交系统在细胞外蛋白质相互作用中的应用.第二军医大学学报24,1095-1098.
Andeol Falcon de, L., Luke, H., Nicolas, L.T., Etienne, D., Claire, L., Murray, B., Millar, A.H., and Ian, S. (2008). The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana. The Plant Journal 56,157-168.
Anne-Laure, C.-B., Maricela, R.-V., Arturo, G.-G, Charles, A., Maria de la Luz, G.-N., Araceli, C., Etienne, D., Luis Felipe, J., Claire, L., Ian, S., and Patricia, L. (2008). CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts. The Plant Journal 56, 590-602.
Backert, S., Lynn Nielsen, B., and Borner, T. (1997). The mystery of the rings: structure and replication of mitochondrial genomes from higher plants. Trends in Plant Science 2,477-483.
Balk,J., and Leaver, C.J. (2001). The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release. Plant Cell 13,1803-1818.
Bergman, P., Edqvist, J., Farbos, I., and Glimelius, K. (2000). Male-sterile tobacco displays abnormal mitochondrial atpl transcript accumulation and reduced floral ATP/ADP ratio. Plant Molecular Biology 42,531-544.
Bonhomme, S., Budar, F., Ferault, M., and Pelletier, G. (1991). A 2.5 kb NcoI fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male-sterility in Brassica cybrids. Curr. Genet.19,121-127.
Bras, M., Queenan, B., and Susin, S. (2005). Programmed cell death via mitochondria:Different modes of dying. Biochemistry (Moscow) 70,231-239.
Brown, G.G., Formanova, N., Jin, H., Wargachuk, R., Dendy, C., Patil, P., Laforest, M., Zhang, J., Cheung, W.Y., and Landry, B.S. (2003). The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35,262-272.
Burger, G., Gray, M.W., and Franz Lang, B. (2003). Mitochondrial genomes: anything goes. Trends in Genetics 19,709-716.
Chase, C.D. (2007). Cytoplasmic male sterility:a window to the world of plant mitochondrial-nuclear interactions. Trends Genet 23,81-90.
Chinnery, P.F. (2003). Searching for nuclear-mitochondrial genes. Trends in Genetics 19,60-62.
Clery, A., Blatter, M., and Allain, F.H. (2008). RNA recognition motifs:boring? Not quite. Curr Opin Struct Biol 18,290-298.
Daniell, H., Kumar, S., and Dufourmantel, N. (2005). Breakthrough in chloroplast genetic engineering of agronomically important crops 23,238-245.
Delannoy, E., Stanley, W.A., Bond, C.S., and Small, I.D. (2007). Pentatricopeptide repeat (PPR) proteins as sequence-specificity factors in post-transcriptional processes in organelles. Biochemical Society Transactions 35,1643-1647.
Desloire, S., Gherbi, H., Laloui, W., Marhadour, S., Clouet, V., Cattolico, L., Falentin, C., Giancola, S., Renard, M., Budar, F., Small, I., Caboche, M., Delourme, R., and Bendahmane, A. (2003). Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family. EMBO Rep 4,588-594.
Ding, Y.-H., Liu, N.-Y., Tang, Z.-S., Liu, J., and Yang, W.-C. (2006). Arabidopsis GLUTAMINE-RICH PROTEIN23 Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase Ⅱ Subunit Ⅲ. Plant Cell 18,815-830.
Duan, S.-H., Li, S.-Q., Li, Y.-S., Xiong, Y., and Zhu, Y.-G. (2007). Distribution and SNPs of the rice CMS-related gene in AA-genome of Oryza species. Yichuan 29,455-461.
Duroc, Y., Hiard, S., Vrielynck, N., Ragu, S., and Budar, F. (2009). The Ogura sterility-inducing protein forms a large complex without interfering with the oxidative phosphorylation components in rapeseed mitochondria. Plant Mol.Biol.70,123-137.
Duroc, Y., Gaillard, C., Hiard, S., Defrance, M.C., Pelletier, G., and Budar, F. (2005). Biochemical and functional characterization of ORF138, a mitochondrial protein responsible for Ogura cytoplasmic male sterility in Brassiceae. Biochimie 87,1089-1100.
Eubel, H., Heazlewood, J.L., and Millar, A.H. (2007). Isolation and subfractionation of plant mitochondria for proteomic analysis. Methods Mol Biol 355,49-62.
Fey, J., and Marechal-Drouard, L. (1999). Compilation and Analysis of Plant Mitochondrial Promoter Sequences:An Illustration of a Divergent Evolution between Monocot and Dicot Mitochondria. Biochemical and Biophysical Research Communications 256,409-414.
Fields, S., and Song, O.-k. (1989). A novel genetic system to detect protein-protein interactions. Nature 340,245-246.
Fisk, D.G., Walker, M.B., and Barkan, A. (1999). Molecular cloning of the maize gene crpl reveals similarity between regulators of mitochondrial and chloroplast gene expression. Embo J 18,2621-2630.
Forner, J., Weber, B., Thuss, S., Wildum, S., and Binder, S. (2007). Mapping of mitochondrial mRNA termini in Arabidopsis thaliana:t-elements contribute to 5'and 3'end formation. Nucl. Acids Res.35,3676-3692.
Fujii, S., and Toriyama, K. (2008). Genome Barriers between Nuclei and Mitochondria Exemplified by Cytoplasmic Male Sterility. Plant Cell Physiol. 49,1484-1494.
Fujii, S., Kazama, T., and Toriyama, K. (2008). Molecular Studies on Cytoplasmic Male Sterility-associated Genes and Restorer Genes in Rice. In Rice Biology in the Genomics Era.Biotechnology in Agriculture and Forestry (Berlin Heidelberg:Springer.
Gieg, P., and Brennicke, A. (2001). From gene to protein in higher plant mitochondria. Life Sciences 324,209-217.
Gillman, J.D., Bentolila, S., and Hanson, M.R. (2007). The petunia restorer of fertility protein is part of a large mitochondrial complex that interacts with transcripts of the CMS-associated locus. Plant J.49,217-227.
Gonzalez-Melendi, P., Uyttewaal, M., Morcillo, C.N., Mora, J.R.H., Fajardo, S., Budar, F., and Lucas, M.M. (2008). A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). Journal of Experimental Botany 59,827-838.
Handa, H., Gualberto, J.M., and Grienenberger, J.-M. (1995). Characterization of the mitochondrial orfB gene and its derivative, orf224, a chimeric open reading frame specific to one mitochondrial genome of the "Polima" male-sterile cytoplasm in rapeseed (Brassica napus L.). Curr. Genet.28, 546-552.
Hanson, M.R., and Bentolila, S. (2004). Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development. Plant Cell 16, S154-169.
Hanson, M.R., Wilson, R.K., Bentolila, S., Kohler, R.H., and Chen, H.C. (1999). Mitochondrial gene organization and expression in petunia male fertile and sterile plants. J Hered 90,362-368.
Hattori, M., Miyake, H., and Sugita, M. (2007). A pentatricopeptide repeat protein is required for RNA processing of clpP pre-mRNA in moss chloroplasts. Journal of Biological Chemistry 282,10773-10782.
Hoang, V.T., Daryl, R.P., Lynn, C.S., Reggie, A.S., Figuhr, R.M., Bin, Y., and Keith, F.S. (1996). Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum. The Plant Journal 10,123-133.
Hook, B., Bernstein, D., Zhang, B., and Wickens, M. (2005). RNA-protein interactions in the yeast three-hybrid system:Affinity, sensitivity, and enhanced library screening. RNA 11,227-233.
Horn, R., Kohler, R.H., and Zetsche, K. (1991). A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol.Biol.17, 29-36.
Hu, J.C., Kornacker, M.G, and Hochschild, A. (2000). Escherichia coli one-and two-hybrid systems for the analysis and identification of protein-protein interactions. Methods 20,80-94.
Ikeda, T.M., and Gray, M.W. (1999). Characterization of a DNA-Binding Protein Implicated in Transcription in Wheat Mitochondria. Mol. Cell. Biol.19, 8113-8122.
Iwabuchi, M., Kyozuka, J., and Shimamoto, K. (1993). Processing followed by complete editing of an altered mitochondrial atp6 RNA restores fertility of cytoplasmic male sterile rice. Embo J 12,1437-1446.
Iwabuchi, M., Koizuka, N., Fujimoto, H., Sakai, T., and Imamura, J. (1999). Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orfl38. Plant Mol.Biol.39,183-188.
Jenny, C., Ulf, L., Jens, S., Rita, T., Frank, W., Elliot, M.M., and Kristina, G. (2007). Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterileBrassica napus flowers. The Plant Journal 49,452-462.
Jobling, M.G, and Holmes, R.K. (2000). Identification of motifs in cholera toxin Al polypeptide that are required for its interaction with human ADP-ribosylation factor 6 in a bacterial two-hybrid system. Proc Natl Acad Sci U S A 97, 14662-14667.
Joung, J.K., Ramm, E.I., and Pabo, C.O. (2000). A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97,7382-7387.
Kadowaki, K., Suzuki, T., and Kazama, S. (1990). A chimeric gene containing the 5'portion of atp6 is associated with cytoplasmic male-sterility of rice. Mol Gen Genet 224,10-16.
Kang, S., Han, J.S., Park, J.H., Skarstad, K., and Hwang, D.S. (2003). SeqA protein stimulates the relaxing and decatenating activities of topoisomerase Ⅳ. J Biol Chem 278,48779-48785.
Kaul, M.L.H. (1988). Male Sterility in Higher Plants. Male Sterility in Higher Plants 4.
Kazama, T., Nakamura, T., Watanabe, M., Sugita, M., and Toriyama, K. (2008). Suppression mechanism of mitochondrial ORF79 accumulation by Rfl protein in BT-type cytoplasmic male sterile rice. The Plant Journal 55,619-628.
Kim, D., Kang, J., and Kim, B.-D. (2007). Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.). Plant Mol.Biol.63,519-532.
Koizuka, N., Imai, R., Fujimoto, H., Hayakawa, T., Kimura, Y., Kohno-Murase, J., Sakai, T., Kawasaki, S., and Imamura, J. (2003). Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J.34, 407-415.
Kotera, E., Tasaka, M., and Shikanai, T. (2005). A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts. Nature 433,326-330.
Kubo, T., and Newton, K.J. (2008). Angiosperm mitochondrial genomes and mutations. Mitochondrion 8,5-14.
Kuhn, J., and Binder, S. (2002). RT-PCR analysis of 5'to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria. Nucl. Acids Res.30,439-446.
Kuhn, J., Tengler, U., and Binder, S. (2001). Transcript Lifetime Is Balanced between Stabilizing Stem-Loop Structures and Degradation-Promoting Polyadenylation in Plant Mitochondria. Mol. Cell. Biol.21,731-742.
Kuhn, K., Weihe, A., and Borner, T. (2005). Multiple promoters are a common feature of mitochondrial genes in Arabidopsis. Nucl. Acids Res.33,337-346.
Kumar, S., Nei, M., Dudley, J., and Tamura, K. (2008). MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9,299-306.
L'Homme, Y., Stahl, R.J., Li, X.-Q., Hameed, A., and Brown, G.G. (1997). Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene. Curr. Genet.31,325-335.
Landgren, M., Zetterstrand, M., Sundberg, E., and Glimelius, K. (1996). Alloplasmic male-sterile Brassica lines containing B. tournefortii mitochondria express an ORF 3 of the atp6 gene and a 32 kDa protein. Plant Mol.Biol.32,879-890.
Lee, H., Kang, S., Bae, S.H., Choi, B.S., and Hwang, D.S. (2001). SeqA protein aggregation is necessary for SeqA function. J Biol Chem 276,34600-34606.
Li, S.Q., Yang, D.C., and Zhu, Y.G. (2007). Characterization and use of male sterility in hybrid rice breeding. J. Integr. Plant Biol.49,791-804.
Li, S.Q., Wan, C.X., Kong, J., Zhang, Z.J., Li, Y.S., and Zhu, Y.G. (2004). Programmed cell death during microgenesis in a Honglian CMS line of rice is correlated with oxidative stress in mitochondria. Funct. Plant Biol.31, 369-376.
Linke, B., and Borner, T. (2005). Mitochondrial effects on flower and pollen development. Mitochondrion 5,389-402.
Liu, F., Cui, X., Horner, H.T., Weiner, H., and Schnable, P.S. (2001). Mitochondrial Aldehyde Dehydrogenase Activity Is Required for Male Fertility in Maize. Plant Cell 13,1063-1078.
Lopian, L., Nussbaum-Shochat, A., O'Day-Kerstein, K., Wright, A., and Amster-Choder, O. (2003). The BglF sensor recruits the BglG transcription regulator to the membrane and releases it on stimulation. Proc Natl Acad Sci U SA100,7099-7104.
Lurin, C., Andres, C., Aubourg, S., Bellaoui, M., Bitton, F., Bruyere, C., Caboche, M., Debast, C., Gualberto, J., Hoffmann, B., Lecharny, A., Le Ret, M., Martin-Magniette, M.-L., Mireau, H., Peeters, N., Renou, J.-P., Szurek, B., Taconnat, L., and Small, I. (2004). Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis. Plant Cell 16,2089-2103.
Mackenzie, S.A., and Chase, C.D. (1990). Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean. Plant Cell 2,905-912.
Manchekar, M., Scissum-Gunn, K., Song, D., Khazi, F., McLean, S.L., and Nielsen, B.L. (2006). DNA Recombination Activity in Soybean Mitochondria. Journal of Molecular Biology 356,288-299.
Manthey, G.M., and McEwen, J.E. (1995). The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae. Embo J14,4031-4043.
Maris, C., Dominguez, C., and Allain, F.H. (2005). The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression. FEBS J 272,2118-2131.
Meierhoff, K., Felder, S., Nakamura, T., Bechtold, N., and Schuster, G. (2003). HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs. Plant Cell 15,1480-1495.
Nakamura, T., and Sugita, M. (2008). A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribonuclease activity. FEBS Lett.582,4163-4168.
O'Toole, N., Hattori, M., Andres, C., Iida, K., Lurin, C., Schmitz-Linneweber, C., Sugita, M., and Small, I. (2008). On the expansion of the pentatricopeptide repeat gene family in plants. Mol. Biol. Evol.25,1120-1128.
Okuda, K., Nakamura, T., Sugita, M., Shimizu, T., and Shikanai, T. (2006). A Pentatricopeptide Repeat Protein Is a Site Recognition Factor in Chloroplast RNA Editing. J. Biol. Chem.281,37661-37667.
Okuda, K., Myouga, F., Motohashi, R., Shinozaki, K., and Shikanai, T. (2007). Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing. Proc. Natl. Acad. Sci. U. S. A.104,8178-8183.
Reichert, A.S., and Neupert, W. (2004). Mitochondriomics or what makes us breathe. Trends in Genetics 20,555-562.
Rossi, F., Charlton, C.A., and Blau, H.M. (1997). Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation. Proc Natl Acad Sci U S A 94,8405-8410.
RP, W., AE, F., DR, P., and BG, G. (1987). urfl3-T of T cytoplasm maize mitochondria encodes a 13kD polypeptide. Plant Mol. Biol.9,121-126.
Sabar, M., Gagliardi, D., Balk, J., and Leaver, C.J. (2003). ORFB is a subunit of F1FO-ATP synthase:insight into the basis of cytoplasmic male sterility in sunflower. EMBO Rep.4,381-386.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning:A Laboratory Manual,2nd edn.
Schmitz-Linneweber, C., and Small, I. (2008). Pentatricopeptide repeat proteins:a socket set for organelle gene expression. Trends in Plant Science 13,663-670.
Schmitz-Linneweber, C., Williams-Carrier, R., and Barkan, A. (2005a). RNA Immunoprecipitation and Microarray Analysis Show a Chloroplast Pentatricopeptide Repeat Protein to Be Associated with the 5'Region of mRNAs Whose Translation It Activates. Plant Cell 17,2791-2804.
Schmitz-Linneweber, C., Williams-Carrier, R., and Barkan, A. (2005b). RNA immunoprecipitation and microarray analysis show a chloroplast pentatricopeptide repeat protein to be associated with the 5'region of mRNAs whose translation it activates. Plant Cell 17,2791-2804.
Schmitz-Linneweber, C., Williams-Carrier, R.E., Williams-Voelker, P.M., Kroeger, T.S., Vichas, A., and Barkan, A. (2006). A Pentatricopeptide Repeat Protein Facilitates the trans-Splicing of the Maize Chloroplast rpsl2 Pre-mRNA. Plant Cell 18,2650-2663.
Segui-Simarro, J.M., Coronado, M.J., and Staehelin, L.A. (2008). The Mitochondrial Cycle of Arabidopsis Shoot Apical Meristem and Leaf Primordium Meristematic Cells Is Defined by a Perinuclear Tentaculate/Cage-Like Mitochondrion. Plant Physiol.148,1380-1393.
Shinjyo, C. (1969). Cytoplasmic genetic male sterility in cultivated rice, Oryza sativa L.. Japanese Journal of Genetics 44,149-156.
Singh, M., and Brown, G.G. (1991). Suppression of Cytoplasmic Male Sterility by Nuclear Genes Alters Expression of a Novel Mitochondrial Gene Region. Plant Cell 3,1349-1362.
Small, I.D., and Peeters, N. (2000). The PPR motif-a TPR-related motif prevalent in plant organellar proteins. Trends Biochem.Sci.25,45-47.
Song, J., and Hedgcoth, C. (1994). A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat. Plant Molecular Biology 26, 535-539.
Tavares-Carreon, F., Camacho-Villasana, Y., Zamudio-Ochoa, A., Shingu-Vazquez, M., Torres-Larios, A., and Perez-Martinez, X. (2008). The pentatricopeptide repeats present in pet309 are necessary for translation but not for stability of the mitochondrial COX1 mRNA in yeast. Journal of Biological Chemistry 283,1472-1479.
Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res.22,4673-4680.
Touzet, P. (2002). Is rf2 a restorer gene of CMS-T in maize? Trends Plant Sci 7,434; author reply 434.
Uyttewaal, M., Mireau, H., Rurek, M., Hammani, K., Arnal, N., Quadrado, M., and Gieg, P. (2008). PPR336 is Associated with Polysomes in Plant Mitochondria. Journal of Molecular Biology 375,626-636.
Wang, Z., Zou, Y., Li, X., Zhang, Q., Chen, L., Wu, H., Su, D., Chen, Y., Guo, J., Luo, D., Long, Y., Zhong, Y., and Liu, Y.-G. (2006). Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing. Plant Cell 18,676-687.
Wen, L., Ruesch, K.L., Ortega, V.M., Kamps, T.L., Gabay-Laughnan, S., and Chase, C.D. (2003). A nuclear restorer-of-fertility mutation disrupts accumulation of mitochondrial ATP synthase subunit alpha in developing pollen of S male-sterile maize. Genetics 165,771-779.
Williams-Carrier, R., Kroeger, T., and Barkan, A. (2008). Sequence-specific binding of a chloroplast pentatricopeptide repeat protein to its native group II intron ligand. RNA14,1930-1941.
Wintz, H., Chen, H.C., Sutton, C.A., Conley, C.A., Cobb, A., Ruth, D., and Hanson, M.R. (1995). Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco. Plant Mol Biol 28,83-92.
Wise, R.P., Bronson, C.R., Schnable, P.S., and Horner, H.T. (1999). The genetics, pathology, and molecular biology of T-cytoplasm male sterility in maize. In Advances in Agronomy, Vol 65 (San Diego:Academic Press Inc), pp.79-130.
Yamamoto, M.P., Shinada, H., Onodera, Y., Komaki, C., Mikami, T., and Kubo, T. (2008). A male sterility-associated mitochondrial protein in wild beets causes pollen disruption in transgenic plants. Plant Journal 54,1027-1036.
Yan, B., and Pring, D.R. (1997). Transcriptional initiation sites in sorghum mitochondrial DNA indicate conserved and variable features. Curr. Genet.32, 287-295.
Yi, P., Wang, L., Sun, Q.P., and Zhu, Y.G. (2002). Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice. Chin. Sci. Bull.47,744-747.
Young, E.G, and Hanson, M.R. (1987). A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50,41-49.
Zhang, Y. (2008). I-TASSER server for protein 3D structure prediction. Bmc Bioinformatics 9,40.
Zhu, Y. (2000). Biology of rice male sterility. (wuhan:Wuhan University Press).
Zhu, Y., Saraike, T., Yamamoto, Y, Hagita, H., Takumi, S., and Murai, K. (2008). orf260(cra), a Novel Mitochondrial Gene, is Associated with the Homeotic Transformation of Stamens into Pistil-Like Structures (Pistillody) in Alloplasmic Wheat. Plant and Cell Physiology 49,1723-1733.
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucl. Acids Res.31,3406-3415.
李绍清.(2004).水稻红莲型细胞质雄性不育与育性恢复的分子机理.武汉大学博士学位论文(武汉：武汉大学).
胡朝凤,彭晓珏,周杨永,谭艳平,李绍清,朱英国.(2008).红莲型水稻杂种F1花药噬菌体展示文库的构建.遗传30,771-775.
彭晓珏.(2008).水稻红莲型细胞质雄性不育基因orfH79的功能鉴定及其分子机理的初步研究.武汉大学博士学位论文(武汉：武汉大学).
张弛,王梁华,罗以勤,张兴群,高云,史薇,包晨颖,球谊,焦炳华.(2003).细菌双杂交系统在细胞外蛋白质相互作用中的应用.第二军医大学学报24,1095-1098.
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