人血浆中PYY定量检测方法的建立
|
摘要
酪酪肽（PYY）与神经肽Y（NPY）和胰多肽（PP）具有较高的序列同源性和结构同源性（PP-折叠），共同属于NPY调节肽家族。PYY是一种含有36个氨基酸的直链多肽，且在回肠末端、结肠和直肠浓度最大。在循环系统中主要以PYY1-36和PYY3-36两种形式存在。目前研究的热点在于它们在肥胖病和糖尿病的发生和治疗等方面的机制和作用。PYY可通过下丘脑食欲调节机制来调节食欲和体重平衡。PYY1-36和PYY3-36通过与不同的Y受体亚型结合在食欲和体重平衡的调节方面发挥相反的作用。前者通过与存在于神经系统中的Y1和Y5受体结合，刺激食欲的产生和促进体重的增加；后者则通过与Y2受体结合抑制食欲和促进体重下降。因此，在定量分析生物基质中PYY水平时区分这两种存在形式对相关疾病的发生机制和诊断等有着重要的意义。
本文以建立血浆PYY定量分析方法为目标，分析了PYY1-36标准曲线法的线性范围、检出限及定量限；分析了用PYY酶解片段（PYY1-19）代替PYY1-36之后方法的改进（色谱分离效率、离子化效率、仪器响应等）；通过对血浆样品进行样品前处理优化，结合超高效液相色谱-串联质谱技术建立了血浆中PYY定量分析的方法，并进行了方法学考察（线性范围、检出限、定量限、精密度和回收率等）。该方法具有快速、灵敏和专一性强的特点。本论文所作的工作及结论如下：
1. PYY1-36校准曲线的建立
应用增强型全扫描和子离子扫描方式确立PYY1-36用于多反应监测法中母-子离子对；优化液相色谱和质谱各项参数；比较并优化血浆PYY1-36的提取方法，结果显示应用HLB柱固相萃取方法的回收率和重复性均高于其他提取方法；采用标准曲线法建立PYY1-36的校准曲线，其线性范围为1000~25000ng/mL，检出限和定量限分别为250ng/mL和1000ng/mL，远高于预期的人血浆中内源性PYY的水平。
2. PYY1-19校准曲线的建立
通过胰蛋白酶消化法得到PYY1-36消化产物之一，PYY1-19；确立PYY1-19用于多反应监测法中的母-子离子对；比较Ultimate3000高效液相色谱系统和Shimadzu超高效液相色谱系统对PYY1-19的分离效率，发现后者在不影响响应值的前提下，洗脱梯度由前者15min缩短到6min，大大提高了分离效率，缩短了样品分析时间；比较PYY1-19和PYY1-36质谱仪器检测的灵敏度，结果证实应用较小片段代替完整PYY能够极大的提高电喷雾离子源对目标物的离子化效率和质谱仪的灵敏度；优化液相色谱和质谱各项参数；采用标准曲线法建立PYY1-19的校准曲线，其线性范围为0.5~250ng/mL，检出限和定量限分别为0.1ng/mL和0.5ng/mL。
3.血浆PYY校准曲线的建立
优化血浆胰蛋白酶消化反应条件以提高血浆中PYY1-19的检测灵敏度，结果表明应用pH8.5100mmol/L碳酸氢铵缓冲液，10μg胰蛋白酶能够完全消化50μL血浆中PYY1-36；各萃取方法回收率比较结果表明，HLB固相萃取法同样适用于血浆PYY1-19的提取；应用标准加入法建立血浆中PYY1-19的校准曲线，线性范围为5~1000ng/mL，检出限和定量限分别为1ng/mL和5ng/mL；比较标准曲线法和标准加入法所得线性回归方程中的斜率，证实基质效应显著，因此血浆PYY定量分析应采用标准加入法来消除基质效应，获得准确可靠的数据。
Peptide YY (PYY) is a member of the Neuropeptide Y (NPY) family of regulatorypeptides that includes NPY, PYY and pancreatic polypeptide (PP) with high sequence andstructural homology (PP-fold). PYY is secreted as a36amino acid, straight chain polypeptide,and is found in greatest concentrations in the terminal ileum, colon and rectum. There are twoforms mainly exist in circulation system, i.e. PYY1-36and PYY3-36. The PYY3-36is aproduct derived from dipeptidyl peptidase IV (DPP-IV) cleaves the N-terminalTyrosine-Proline residues from PYY1-36. The current studies of PYY are focus on the effectsof PYY on obesity and diabetes development and treatment. PYY participates in theregulation of appetite and weight balance through hypothalamic-based mechanisms. PYY1-36and PYY3-36cause opposite effects by acting through different Y-receptor sub-types.PYY1-36acts through Y1and Y5receptors in the central nervous system to stimulate appetiteand to promote weight increase, while PYY3-36binds Y2-receptors, which inhibit appetiteand promote weight loss. So it is very important to distinguish these two forms when quantifyPYY levels in biological matrices.
In the present study, to establish PYYquantitative analysis method in human plasma, theperformance of PYY1-36calibration curve constructed by standard curve method wasevaluated; digested PYY was prepared and used to improve the method (chromatographicseparation efficiency, ionization efficiency, instrument response, etc.); ultra-high performanceliquid chromatography-tandem mass spectrometry method was established for PYYquantitative analysis in plasma and validated with a set of parameters (linearity, precision,recovery, limit of detection and limit of quantitation). The thesis included the following workand conclusions:
1. Construction of calibration curve of PYY1-36
Combination of enhanced full-scan and product ion scan mode to establish multiplereaction monitoring (MRM) method transtitions for intact PYY1-36quantitation. Theparameters of liquid chromatography and mass spectrometry were optimized. The extractionprotocols for PYY were compared and optimized, and the comparison result of recovery andreproducibility showed that the SPE HLB cartridge extraction protocol was the best method toextact PYY1-36from human plasma. The standard curve was constructed by standard curve method, and the linear range was250~1000ng/mL with limit of detection and limit ofquantitation were100ng/mL and250ng/mL, respectively, which much higher than theexpected endogenous level of PYY in human plasma.
2. Construction of standard curve of PYY1-19
The fragment PYY1-19was prepared by digested PYY1-36with trypsin. MRMtransitions for PYY1-19were established by enhanced full-scan and product ion scan mode.The separation efficiency of two liquid chromatography systems, i.e. Ultimate3000highperformance liquid chromatography system (HPLC) and Shimadzu ultra-high performanceliquid chromatography system (UPLC) was compared, and the result showed that the UPLCsystem could improve the separation efficiency greatly (elution gradient reduced from15minto6min) without affecting the response of the instrument to PYY1-19; Mass spectrometerdetection sensitivity for PYY1-19and PYY1-36was analyzed, the result confirm thatapplication of digested small fragments instead of intact PYY can greatly improve ionizationefficiency of the electrospray ion source and increase mass spectrometer sensitivity. Theconditions for better liquid chromatographic separation and mass spectrometry instrumentsensitivity were optimized. The standard curve was constructed by standard curve method.The linear range was0.5~250ng/mL with limit of detection and limit of quantitation were0.1ng/mL and0.5ng/mL, respectively.
3. Establishment of calibration curve of PYY1-19in human plasma
The trypsin digestion condition for completely digestion of PYY in human plasma wasoptimized. The calibration curve of PYY1-19in human plasma was constructed by standardaddition method, the linear range was5~1000ng/mL with limit of detection and limit ofquantitation were1ng/mL and5ng/mL, respectively. Comparison of the calibration curvesprepared in standard solution and complex matrix (human plasma) showed that there was asignificant matrix effect, in consequence, it should use the standard addition method whenquantify the PYY level in human plasma.
本文以建立血浆PYY定量分析方法为目标，分析了PYY1-36标准曲线法的线性范围、检出限及定量限；分析了用PYY酶解片段（PYY1-19）代替PYY1-36之后方法的改进（色谱分离效率、离子化效率、仪器响应等）；通过对血浆样品进行样品前处理优化，结合超高效液相色谱-串联质谱技术建立了血浆中PYY定量分析的方法，并进行了方法学考察（线性范围、检出限、定量限、精密度和回收率等）。该方法具有快速、灵敏和专一性强的特点。本论文所作的工作及结论如下：
1. PYY1-36校准曲线的建立
应用增强型全扫描和子离子扫描方式确立PYY1-36用于多反应监测法中母-子离子对；优化液相色谱和质谱各项参数；比较并优化血浆PYY1-36的提取方法，结果显示应用HLB柱固相萃取方法的回收率和重复性均高于其他提取方法；采用标准曲线法建立PYY1-36的校准曲线，其线性范围为1000~25000ng/mL，检出限和定量限分别为250ng/mL和1000ng/mL，远高于预期的人血浆中内源性PYY的水平。
2. PYY1-19校准曲线的建立
通过胰蛋白酶消化法得到PYY1-36消化产物之一，PYY1-19；确立PYY1-19用于多反应监测法中的母-子离子对；比较Ultimate3000高效液相色谱系统和Shimadzu超高效液相色谱系统对PYY1-19的分离效率，发现后者在不影响响应值的前提下，洗脱梯度由前者15min缩短到6min，大大提高了分离效率，缩短了样品分析时间；比较PYY1-19和PYY1-36质谱仪器检测的灵敏度，结果证实应用较小片段代替完整PYY能够极大的提高电喷雾离子源对目标物的离子化效率和质谱仪的灵敏度；优化液相色谱和质谱各项参数；采用标准曲线法建立PYY1-19的校准曲线，其线性范围为0.5~250ng/mL，检出限和定量限分别为0.1ng/mL和0.5ng/mL。
3.血浆PYY校准曲线的建立
优化血浆胰蛋白酶消化反应条件以提高血浆中PYY1-19的检测灵敏度，结果表明应用pH8.5100mmol/L碳酸氢铵缓冲液，10μg胰蛋白酶能够完全消化50μL血浆中PYY1-36；各萃取方法回收率比较结果表明，HLB固相萃取法同样适用于血浆PYY1-19的提取；应用标准加入法建立血浆中PYY1-19的校准曲线，线性范围为5~1000ng/mL，检出限和定量限分别为1ng/mL和5ng/mL；比较标准曲线法和标准加入法所得线性回归方程中的斜率，证实基质效应显著，因此血浆PYY定量分析应采用标准加入法来消除基质效应，获得准确可靠的数据。
Peptide YY (PYY) is a member of the Neuropeptide Y (NPY) family of regulatorypeptides that includes NPY, PYY and pancreatic polypeptide (PP) with high sequence andstructural homology (PP-fold). PYY is secreted as a36amino acid, straight chain polypeptide,and is found in greatest concentrations in the terminal ileum, colon and rectum. There are twoforms mainly exist in circulation system, i.e. PYY1-36and PYY3-36. The PYY3-36is aproduct derived from dipeptidyl peptidase IV (DPP-IV) cleaves the N-terminalTyrosine-Proline residues from PYY1-36. The current studies of PYY are focus on the effectsof PYY on obesity and diabetes development and treatment. PYY participates in theregulation of appetite and weight balance through hypothalamic-based mechanisms. PYY1-36and PYY3-36cause opposite effects by acting through different Y-receptor sub-types.PYY1-36acts through Y1and Y5receptors in the central nervous system to stimulate appetiteand to promote weight increase, while PYY3-36binds Y2-receptors, which inhibit appetiteand promote weight loss. So it is very important to distinguish these two forms when quantifyPYY levels in biological matrices.
In the present study, to establish PYYquantitative analysis method in human plasma, theperformance of PYY1-36calibration curve constructed by standard curve method wasevaluated; digested PYY was prepared and used to improve the method (chromatographicseparation efficiency, ionization efficiency, instrument response, etc.); ultra-high performanceliquid chromatography-tandem mass spectrometry method was established for PYYquantitative analysis in plasma and validated with a set of parameters (linearity, precision,recovery, limit of detection and limit of quantitation). The thesis included the following workand conclusions:
1. Construction of calibration curve of PYY1-36
Combination of enhanced full-scan and product ion scan mode to establish multiplereaction monitoring (MRM) method transtitions for intact PYY1-36quantitation. Theparameters of liquid chromatography and mass spectrometry were optimized. The extractionprotocols for PYY were compared and optimized, and the comparison result of recovery andreproducibility showed that the SPE HLB cartridge extraction protocol was the best method toextact PYY1-36from human plasma. The standard curve was constructed by standard curve method, and the linear range was250~1000ng/mL with limit of detection and limit ofquantitation were100ng/mL and250ng/mL, respectively, which much higher than theexpected endogenous level of PYY in human plasma.
2. Construction of standard curve of PYY1-19
The fragment PYY1-19was prepared by digested PYY1-36with trypsin. MRMtransitions for PYY1-19were established by enhanced full-scan and product ion scan mode.The separation efficiency of two liquid chromatography systems, i.e. Ultimate3000highperformance liquid chromatography system (HPLC) and Shimadzu ultra-high performanceliquid chromatography system (UPLC) was compared, and the result showed that the UPLCsystem could improve the separation efficiency greatly (elution gradient reduced from15minto6min) without affecting the response of the instrument to PYY1-19; Mass spectrometerdetection sensitivity for PYY1-19and PYY1-36was analyzed, the result confirm thatapplication of digested small fragments instead of intact PYY can greatly improve ionizationefficiency of the electrospray ion source and increase mass spectrometer sensitivity. Theconditions for better liquid chromatographic separation and mass spectrometry instrumentsensitivity were optimized. The standard curve was constructed by standard curve method.The linear range was0.5~250ng/mL with limit of detection and limit of quantitation were0.1ng/mL and0.5ng/mL, respectively.
3. Establishment of calibration curve of PYY1-19in human plasma
The trypsin digestion condition for completely digestion of PYY in human plasma wasoptimized. The calibration curve of PYY1-19in human plasma was constructed by standardaddition method, the linear range was5~1000ng/mL with limit of detection and limit ofquantitation were1ng/mL and5ng/mL, respectively. Comparison of the calibration curvesprepared in standard solution and complex matrix (human plasma) showed that there was asignificant matrix effect, in consequence, it should use the standard addition method whenquantify the PYY level in human plasma.
引文
Abbott C R, Small C J, Kennedy A R, Neary N M, Sajedi A, Ghatei M A, Bloom S R.2005. Blockade ofthe neuropeptide Y Y2receptor with the specific antagonist BIIE0246attenuates the effect ofendogenous and exogenous peptide YY(3-36) on food intake. Brain research,1043(1-2):139~144
Adrian T E, Bacarese-Hamilton A J, Allen J M, Tatemoto K, Ferri G L, Polak J M, Bloom S R.1982.Distribution of PYY in the porcine and human gastrointestinal tract. Regulatory Peptides,4(6):355
Adrian T E, Savage A P, Sagor G R, Allen J M, Bacarese-Hamilton A J, Tatemoto K, polak J M, Bloom S R.1985. Effect of peptide YY on gastric, pancreatic, and biliary function in humans. Gastroenterology,89(3):494~499
Adrian T E, Sagor G R, Savage A P, Bacarese-Hamilton A J, Hall G M, Bloom S R.1986a. Peptide YYkinetics and effects on blood pressure and circulating pancreatic and gastrointestinal hormones andmetabolites in man. The Journal of clinical endocrinology and metabolism,63(4):803~807
Adrian T E, Savage A P, Bacarese-Hamilton A J, Wolfe K, Besterman H S, Bloom S R.1986b. PeptideYY abnormalities in gastrointestinal diseases. Gastroenterology,90(2):379~384
Adrian T E, Ballantyne G H, Longo W E, Bilchik A J, Graham S, Basson M D, Tierney R P, Modlin I M.1993. Deoxycholate is an important releaser of peptide-YY and enteroglucagon from the human colon.Gut,34(9):1219~1224
Ali-Rachedi A, Varndell I M, Adrian T E, Gapp D A, Van Noorden S, Bloom S R, Polak J M.1984. PeptideYY (PYY) immunoreactivity is co-stored with glucagon-related immunoreactants in endocrine cells ofthe gut and pancreas. Histochemistry,80(5):487~491
Anderson D S, Kirchner M, Kellogg M, Kalish L A, Jeong, J Y, Vanasse G, Berliner N, Fleming M D,Steen H.2011. Design and validation of a high-throughput matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry method for quantification of Hepcidin in human plasma. Analyticalchemistry,83(21):8357~8362
Anderson N L., Anderson N G, Haines L R, Hardie D B, Olafson R W, Pearson T W.2004. Massspectrometric quantitation of peptides and proteins using stable isotope standards and capture byanti-peptide antibodies (SISCAPA). Journal of Proteome Research,3(2):235~244
Andren P E, Caprioli R M.1999. Determination of extracellular release of neurotensin in discrete rat brainregions utilizing in vivo microdialysis/electrospray mass spectrometry. Brain research,845(2):123~129
Aponte G W, Fink A S, Meyer J H, Tatemoto K, Taylor I L.1985. Regional distribution and release ofpeptide YY with fatty acids of different chain length. The American journal of physiology,249(6Pt1):G745~750
Aristoteli L P, Molloy M P, Baker M S.2007. Evaluation of endogenous plasma peptide extraction methodsfor mass spectrometric biomarker discovery. Journal of Proteome Research,6(2):571~581
Armstrong D N, Krenz H K, Modlin I M, Ballantyne G H.1993. Bile-salt inhibition of motility in theisolated perfused rabbit terminal ileum. Gut,34(4):483~488
Aslan K, Grell T A J.2011. Rapid and sensitive detection of Troponin I in human whole blood samples byusing silver nanoparticle films and microwave heating clinical chemistry,57(5):746~752
Bagshaw R D, Callahan J W, Mahuran D J.2000. Desalting of in-gel-digested protein sample withmini-C18columns for matrix-assisted laser desorption ionization time of flight peptide massfingerprinting. Analytical Biochemistry,284(2):432~435
Ballantyne G H, Longo W E, Savoca P E, Adrian T E, Vukasin A P, Bilchik A J, Sussman J, Modlin I M.1989. Deoxycholate-stimulated release of peptide YY from the isolated perfused rabbit left colon. TheAmerican journal of physiology,257(5Pt1): G715~724
Ballantyne G H, Goldenring J R, Fleming F X, Rush S, Flint J S, Fielding L P, Binder H J, Modlin I M.1993a. Inhibition of VIP-stimulated ion-transport by a novel Y-receptor phenotype in rabbit distalcolon. American Journal of Physiology,264(5): G848~G854
Ballantyne G H, Goldenring J R, Savoca P E, Kranz H K, Adrian T E, Bilchik A J, Modlin I M.1993b.Cyclic AMP-mediated release of peptide-YY(PYY) from the isolated perfused rabbit distal colon.Regulatory Peptides,47(2):117~126
Ballantyne G H, A. Gumbs, Modlin I M.2005. Changes in insulin resistance following bariatric surgeryand the adipoinsular axis: Role of the adipocytokines, leptin, adiponectin and resistin. Obesity Surgery,15(5):692~699
Ballantyne G H.2006. Peptide YY(1-36) and peptide YY(3-36): Part I. Distribution, release and actions.Obesity Surgery,16(5):651~658
Barnidge D R, Goodmanson M K, Klee G G, Muddiman D C.2004. Absolute quantification of the modelbiomarker prostate-specific antigen in serum by LC-MS/MS using protein cleavage and isotopedilution mass spectrometry. Journal of Proteome Research,3(3):644~652
Bartolome M A, Borque M, Martinez-Sarmiento J, Aparicio E, Hernandez C, Cabrerizo L,Fernandez-Represa J A.2002. Peptide YY secretion in morbidly obese patients before and aftervertical banded gastroplasty. Obesity Surgery,12(3):324~327
Batterham R L, Cowley M A, Small C J, Herzog H, Cohen M A, Dakin C L, Wren A M, Brynes A E, LowM J, Ghatei M A, Cone R D, Bloom S R.2002. Gut hormone PYY3-36physiologically inhibits foodintake. Nature,418(6898):650~654
Batterham R L, Cohen M A, Ellis S M, Le Roux C W, Withers G J, Frost G S, Ghatei M A, Bloom S R.2003. Inhibition of food intake in obese subjects by peptide YY3-36. New England Journal ofMedicine,349(10):941~948
Beavis R C, Chaudhary T, Chait B T.1992. Alpha-cyano-4-hydroxycinnamic acid as a matrix formatrix-assited laser desorption mass-spectrometry. Organic Mass Spectrometry,27(2):156~158
Becher F, Pruvost A, Clement G, Tabet J C, Ezan E.2006. Quantification of small therapeutic proteins inplasma by liquid chromatography-Tandem mass spectrometry: Application to an elastase inhibitorEPI-hNE4. Analytical Chemistry,78(7):2306~2313
Beck E J, Tapsell L C, Batterham M J, Tosh S A, Huang X F.2009. Increases in peptide Y-Y levelsfollowing oat beta-glucan ingestion are dose-dependent in overweight adults. Nutrition Research,29(10):705~709
Berglund M M, Hipskind P A, Gehlert D R.2003. Recent developments in our understanding of thephysiological role of PP-fold peptide receptor subtypes. Experimental Biology and Medicine,228(3):217~244
Blundell T L, Pitts J E, Tickle I J, Wood S P, Wu C W.1981. X-ray analysis (1.4-A resolution) of avianpancreatic polypeptide: Small globular protein hormone. Proceedings of the National Academy ofSciences of the United States of America,78(7):4175~4179
Bornsen K O.2000. Influence of salts, buffers, detergents, solvents, and matrices on MALDI-MS proteinanalysis in complex mixtures. Methods in molecular biology,146:387~404
Bottcher G, Alumets J, Hakanson R, Sundler F.1986. Co-existence of glicentin and peptide YY incolorectal L-cells in cat and man. An electron microscopic study. Regulatory Peptides,13(3-4):283~291
Bridges T E, Marino V.1987. A rapid and sensitive method for the identification and quantitation ofsub-picomolar amounts of neurohypophysial peptides. Life sciences,41(26):2815~2822
Bronsky J., Nedvidkova J, Krasnicanova H, Vesela M, Schmidtova J, Koutek J, Kellermayer R, Chada M,Kabelka Z, Hrdlicka M, Nevoral J, Prusa R.2011. Changes of Orexin A Plasma Levels in Girls withAnorexia Nervosa During Eight Weeks of Realimentation. International Journal of Eating Disorders,44(6):547~552
Calderon-Santiago M, Mata-Granados J M, Priego-Capote F, Quesada-Gomez J M, De Castro M D L.2011.Analytical platform for verification and quantitation of target peptides in human serum: Application tocathelicidin. Analytical Biochemistry,415(1):39~45
Chen J L, Huang Y J, Pan C H, Hu C W, Chao M R.2011. Determination of urinary malondialdehyde byisotope dilution LC-MS/MS with automated solid-phase extraction: A cautionary note onderivatization optimization. Free radical biology and medicine,51(9):1823~1829
Clarke N J, Naylor S.2002. Capillary isoelectric focusing-mass spectrometry: analysis of protein mixturesfrom human body fluids. Biomedical Chromatography,16(4):287~297
Cool D R, DeBrosse D.2003. Extraction of oxytocin and arginine-vasopressin from serum and plasma forradioimmunoassay and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry.Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences,792(2):375~380
Corona G, Elia C, Casetta B, Frustaci S, Toffoli G.2011. High-throughput plasma docetaxel quantificationby liquid chromatography-tandem mass spectrometry. Clinica Chimica Acta,412(3-4):358~364
Cowles C L, Zhu X.2011. Sensitive detection of cardiac biomarker using ZnS nanoparticles as novel signaltransducers. Biosensors&bioelectronics,30(1):342~346
Cox H M, Cuthbert A W, Hakanson R, Wahlestedt C.1988. The effect of neuropeptide Y and peptide YYon electrogenic ion transport in rat intestinal epithelia. The Journal of physiology,398:65~80
Cuche G, Cuber J C, Malbert C H.2000. Ileal short-chain fatty acids inhibit gastric motility by a humoralpathway. American Journal of Physiology-Gastrointestinal and Liver Physiology,279(5): G925~G930
Dams R, Huestis M A, Lambert W E, Murphy C M.2003. Matrix effect in bio-analysis of illicit drugs withLC-MS/MS: Influence of ionization type, sample preparation, and biofluid. Journal of the AmericanSociety for Mass Spectrometry,14(11):1290~1294
De Hoog C L, Mann M.2004. Proteomics. Annual Review of Genomics and Human Genetics,5:267~293
Deacon C F.2004. Circulation and degradation of GIP and GLP-1. Hormone and metabolic research,36(11-12):761~765.
Degen L, Drewe J, Piccoli F, Grani K, Oesch S, Bunea R, Amato D M, Beglinger C.2007. Effect of CCK-1receptor blockade on ghrelin and PYY secretion in men. American Journal of Physiology-RegulatoryIntegrative and Comparative Physiology,292(4): R1391~R1399
Deng X Y, Guarita D R, Pedroso M R A, Kreiss C, Wood P G, Sved A F, Whitcomb D C.2001. PYYinhibits CCK-stimulated pancreatic secretion through the area postrema in unanesthetized rats.American Journal of Physiology-Regulatory Integrative and Comparative Physiology,281(2):R645~R653
Domon B, Aebersold R.2006. Review-Mass spectrometry and protein analysis. Science,312(5771):212~217
Druce M R, Small C J, Bloom S R.2004. Minireview: Gut peptides regulating satiety. Endocrinology,145(6):2660~2665
Emotte C, Deglave F, Heudi O, Picard F, Kretz O.2012. Fast simultaneous quantitative analysis of FTY720and its metabolite FTY720-P in human blood by on-line solid phase extraction coupled with liquidchromatography-tandem mass spectrometry. Journal of pharmaceutical and biomedical analysis,58:102~112
Erve J C L, Beyer C E, Manzino L, Talaat R E.2009. Metabolite identification in rat brain microdialysatesby direct infusion nanoelectrospray ionization after desalting on a ZipTip and LTQ/Orbitrap massspectrometry. Rapid Communications in Mass Spectrometry,23(24):4003~4012
Feltrin K L, Little T J, Meyer J H, Horowitz M, Rades T, Wishart J, Feinle-Bisset C.2008. Comparativeeffects of intraduodenal infusions of lauric and oleic acids on antropyloroduodenal motility, plasmacholecystokinin and peptide YY, appetite, and energy intake in healthy men. American Journal ofClinical Nutrition,87(5):1181~1187
Ferri G L, Adrian T E, Allen J M, Soimero L, Cancallieri A, Yeats J C, Blank M, Polak J M, Bloom S R.1988. Intramural distribution of regulatory peptides in the sigmoid-recto-anal region of the human gut.Gut,29(6):762~768
Frankevich V, Zhang J, Dashtiev M, Zenobi R.2003. Production and fragmentation of multiply chargedions in 'electron-free' matrix-assisted laser desorption/ionization. Rapid Communications in MassSpectrometry,17(20):2343~2348
FuCheng X M, Chariot J, Castex N, Galmiche J P, Roze C.1997. Mechanisms of peptide YY releaseinduced by an intraduodenal meal in rats: Neural regulation by proximal gut. PflugersArchiv-European Journal of Physiology,433(5):571~579
Gehlert D R.1998. Multiple receptors for the pancreatic polypeptide (PP-fold) family: Physiologicalimplications. Proceedings of the Society for Experimental Biology and Medicine,218(1):7~22
Goda R, Masumoto H, Okazaki O, Sudo K.2008. Development of a pretreatment method for amyloidbeta-protein analysis based on the effect of acetic acid on the dissolution of plasma polypeptides.Biomedical Chromatography,22(11):1279~1287
Grandt D, Schimiczek M, Beglinger C, Layer P, Goebell H, Eysselein V E, Reeve J R.1994a.2molecular-forms of peptide YY(PYY) are abundant in human blood-characterization of aradioimmunoassay recognizing PYY-1-36and PYY-3-36. Regulatory Peptides,51(2):151~159
Grandt D, Schimiczek M, Struk K, Shively J, Eysselein V E, Goebell H, Reeve J R.1994b.Characterization of2forms of peptide-YY,PYY(1-36) and PYY(3-36), in the rabbit. Peptides,15(5):815~820
Greeley G H Jr, Hashimoto T, Lzukura M, Gomes G, Jeng J, Hill F L, Liuis F, Thompson J C.1989a. Acomparison of intraduodenally and intracolonically administered nutrients on the release ofpeptide-YY in the dog. Endocrinology,125(4):1761~1765
Greeley G H Jr, Jeng J, Gomes G, Hashimoto T, Hill F L, Kern K, Kurosky T, Chuo H F, Thompson J C.1989b. Evidence for regulation of peptide-YY release by the proximal gut. Endocrinology,124(3):1438~1443
Greening D W, Simpson R J.2010. A centrifugal ultrafiltration strategy for isolating the low-molecularweight (<=25K) component of human plasma proteome. Journal of Proteomics,73(3):637~648
Gregor P, Feng Y, Decarr L B, Cornfield L J, Mccaleb M L.1996. Molecular characterization of a secondmouse pancreatic polypeptide receptor and its inactivated human homologue. Journal of BiologicalChemistry,271(44):27776~27781
Grimes J H, O'Connell T M.2011. The application of micro-coil NMR probe technology to metabolomicsof urine and serum. Journal of Biomolecular NMR,49(3-4):297~305
Guo Y S, Singh P, Debouno J F, Thompson J C.1988. Effect of peptide YY on insulin release stimulated by2-deoxyglucose and neuropeptides in dogs. Pancreas,3(2):128~134
Guo Y S, Singh P, Draviam E, Greeley G H Jr, Thompson J C.1989. Peptide YY inhibits the insulinotropicaction of gastric inhibitory polypeptide. Gastroenterology,96(3):690~694
Hagan M M, Moss D E.1995. Effect of Peptide YY (PYY) in food-associated conflict. Physiology&Behavior,58(4):731~735
Halfinger B, Sarg B, Amann A, Hammerer-Lercher A, Lindner H.2011. Unmasking low-abundancepeptides from human blood plasma and serum samples by a simple and robust two-stepprecipitation/immunoaffinity enrichment method. Electrophoresis,32(13):1706~1714
Hanusch-Enserer U, Roden M.2005. News in gut-brain communication: a role of peptide YY (PYY) inhuman obesity and following bariatric surgery? European Journal of Clinical Investigation,35(7):425~430
Heinig K, Wirz T, Yuan M, Tingler M, Mylott W.2011. An improved LC-MS/MS method for thedetermination of taspoglutide in plasma and urine using orthogonal HILIC-RP column switching,ultra-performance LC separation and 'wrong-way-round' electrospray ionization. BiomedicalChromatography,25(11):1215~1221
Heisler T, Towfigh S, Simon N, McFadden D W.2000. Peptide YY and vitamin E inhibithormone-sensitive and-insensitive breast cancer cells. Journal of Surgical Research,91(1):9~14
Hernandez E J, Whitcomb D C, Vigna S R, Taylor I L.1994. Saturable binding of circulating Peptide YY inthe dorsal vagal complex of rats. American Journal of Physiology,266(3): G511~G516
Hosotani R, Inoue K, Kogire M, Tatemoto K, Mutt V, Suzuki T, Rayford P L, Tobe T.1989. Effect ofnatural peptide YY on pancreatic secretion and cholecystokinin release in conscious dogs. Digestivediseases and sciences34(3):468~473
Huang K J, Jing Q S, Wei C Y, W Y Y.2011. Spectrofluorimetric determination of glutathione in humanplasma by solid-phase extraction using graphene as adsorbent. Spectrochimica Acta Part a-Molecularand Biomolecular Spectroscopy,79(5):1860~1865
Janzen N, Sander S, Terhardt M, Steuerwald U, Peter M, Das A M, Sander J.2011. Rapid steroid hormonequantification for congenital adrenal hyperplasia (CAH) in dried blood spots using UPLC liquidchromatography-tandem mass spectrometry. Steroids,76(13):1437~1442
Jian W, Romm M V, Edom R W, Miller V P, Lamarr W A, Weng N D.2011. Evaluation of ahigh-throughput online solid phase extraction-tandem mass spectrometry system for in vivobioanalytical studies. Analytical chemistry,83(21):8259~8266
John H, John S, Forssmann W G.2008. Kinetic studies on aminopeptidase M-mediated degradation ofhuman hemorphin LVV-H7and its N-terminally truncated products. Journal of Peptide Science,14(7):797~803
Jorgensen T J D, Bojesen G, Rahbek-Nielsen H.1998. The proton affinities of seven matrix-assisted laserdesorption/ionization matrices correlated with the formation of multiply charged ions. European MassSpectrometry,4(1):39~45
Kalra B, Kumar A, Patel K, Patel A, Khosravi K.2010. Development of a second generation Inhibin BELISA. Journal of Immunological Methods,362(1-2):22~31
Kanatani A, Mashiko S, Murai N, Sugimoto N, Ito J, Fukuroda T, Fukami T, Morin N, MacNeil D J, Vander Ploeg L H T, Saga Y, Nishimura S, Ihara M.2000. Role of the Y1receptor in the regulation ofneuropeptide Y-mediated feeding: Comparison of wild-type, Y1receptor-deficient, and Y5receptor-deficient mice. Endocrinology,141(3):1011~1016
Kay R, Barton C, Ratcliffe L, Matharoo-Ball B, Brown P, Roberts J, Teale P, Creaser C.2008. Enrichmentof low molecular weight serum proteins using acetonitrile precipitation for mass spectrometry basedproteomic analysis. Rapid Communications in Mass Spectrometry,22(20):3255~3260
Kazanjian K K, Towfigh S, et al.(2003). Peptide YY exhibits a mitogenic effect on pancreatic cells whileimproving acute pancreatitis in vitro. Journal of Surgical Research114(1):95~99
Keil-Dlouha V, Zylber N, Imhoff J M, Tong N T, Keil B.1971. Proteolytic activity of pseudotrypsin.FEBS Letters,16(4):291~295
Keire, D. A., P. Mannon, McFadden D W.2000. Primary structures of PYY, Pro(34) PYY, and PYY-(3-36)confer different conformations and receptor selectivity. American Journal ofPhysiology-Gastrointestinal and Liver Physiology,279(1): G126~G131
Kim B J, Carlson O D, Jang H J, Elahi D, Berry C, Egan J M.2005. Peptide YY is secreted after oralglucose administration in a gender-specific manner. Journal of Clinical Endocrinology&Metabolism,90(12):6665~6671
Koch T R, Roddy D R, Carney J A, Go V L.1988. Peptide YY concentrations in normal ileum and colonand in idiopathic inflammatory bowel disease. Digestive diseases and sciences,33(10):1322~1328
Konturek S J, Pepera J, Zabielski K, Konturek P C, Pawlik T, Szlachcic A, Hahn E G.2003. Brain-gut axisin pancreatic secretion and appetite control. Journal of Physiology and Pharmacology,54(3):293~317
Koomen J M, Li D H, Xiao L C, Liu T C, Coombes K R, Abbruzzese J, Kobayashi R.2005. Direct tandemmass spectrometry reveals limitations in protein profiling experiments for plasma biomarker discovery.Journal of Proteome Research,4(3):972~981
Korner J, Bessler M, Cirilo L J, Conwell I M, Daud A, Restuccia N L, Wardlaw S L.2005. Effects ofRoux-en-Y gastric bypass surgery on fasting and postprandial concentrations of plasma ghrelin,peptide YY, and insulin. Journal of Clinical Endocrinology&Metabolism,90(1):359~365
Korner J, Inabnet W, Conwell I M, Taveras C, Daud A, Olivero-Rivera L, Restuccia N L, Bessler M.2006.Differential effects of gastric bypass and banding on circulating gut hormone and leptin levels. Obesity,14(9):1553~1561
Kuhn E, Wu J, Karl J, Liao H, Zolg W, Guild B.2004. Quantification of C-reactive protein in the serum ofpatients with rheumatoid arthritis using multiple reaction monitoring mass spectrometry andC-13-labeled peptide standards. Proteomics,4(4):1175~1186
Kukucka M A, Misra H P.1994. Determination of oxytocin in biological samples by isocratichigh-performance-liquid chromatography with coulometric detection using C-18solid-phaseextraction and polyclonal antibody-based immunoaffinity column purification. Journal ofChromatography B-Biomedical Applications,653(2):139~145
Kuroda Y, Yukinaga H, Kitano M, Noguchi T, Nemati M, Shibukawa A, Nakagawa T, Matsuzaki K.2005.On-line capillary isoelectric focusing-mass spectrometry for quantitative analysis of peptides andproteins. Journal of pharmaceutical and biomedical analysis,37(3):423~428
Lame M E, Chambers E E, Blatnik M.2011. Quantitation of amyloid beta peptides Aβ1–38, Aβ1–40, andAβ1–42in human cerebrospinal fluid by ultra-performance liquid chromatography–tandem massspectrometry. Analytical Biochemistry,419(2):133~139
Larhammar D.1996. Structural diversity of receptors for neuropeptide Y, peptide YY and pancreaticpolypeptide. Regulatory Peptides,65(3):165~174
Larsimont V, Bogaards M, Mulleneers P, Hochhaus G.1999. Analysis of leucine enkephalin byhigh-performance liquid chromatography using enzymatic derivatization by tyrosinase andelectrochemical or fluorescence detection. Journal of pharmaceutical and biomedical analysis,19(6):855~864
Le Roux C W, Batterham R L, Aylwin S J B, Patterson M, Borg C M, Wynne K J, Kent A, Vincent R P,Gardiner J, Ghatei M A, Bloom S R.2006. Attenuated peptide YY release in obese subjects isassociated with reduced satiety. Endocrinology,147(1):3~8
Li H Y, Rose M J, Holder J R, Wright M, Miranda L P, James C A.2011. Direct Quantitative Analysis of a20kDa PEGylated Human Calcitonin Gene Peptide Antagonist in Cynomolgus Monkey Serum UsingIn-Source CID and UPLC-MS/MS. Journal of the American Society for Mass Spectrometry,22(9):1660~1667.
Lin H C, Zhao X T, Wang L J, Wong H.1996. Fat-induced ileal brake in the dog depends on peptide YY.Gastroenterology,110(5):1491~1495
Lin T M, Chance R E.1974. Candidate hormones of the gut. VI. Bovine pancreatic polypeptide (BPP) andavian pancreatic polypeptide (APP). Gastroenterology,67(4):737~738
Lluis F, Gomez G, Fujimura M, Greeley G H Jr, Thompson J C.1987. Peptide YY inhibits nutrient-,hormonal-, and vagally-stimulated pancreatic exocrine secretion. Pancreas,2(4):454~462
Lluis F, Gomez G, Fujimura M, Greeley G H Jr, Thompson J C.1988. Peptide YY inhibits pancreaticsecretion by inhibiting cholecystokinin release in the dog. Gastroenterology,94(1):137~144
Longo W E, Ballantyne G H, Savoca P E, Adrian T E, Bilchik A J, Modlin I M.1991. Short-chain fatty acidrelease of peptide YY in the isolated rabbit distal colon. Scandinavian journal of gastroenterology,26(4):442~448
Lugari R, Dei Cas A, Ugolotti D, Barilli A L, Camellini C, Ganzerla G C, Luciani A, Salerni B,Mittenperger F, Nodari S, Gnudi A, Zandomeneghi R.2004. Glucagon-like peptide1(GLP-1)secretion and plasma dipeptidyl peptidase IV (DPP-IV) activity in morbidly obese patients undergoingbiliopancreatic diversion. Hormone and Metabolic Research,36(2):111~115
Lundberg J M, Tatemoto K.1982. Vascular effects of the peptides PYY and PHI: comparison with APPand VIP. European journal of pharmacology,83(1-2):143~146
Lundberg J M, Tatemoto K, TereniusL, Hellstrom P M, Mutt V, Hokfelt T, Hamberger B.1982.Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal bloodflow and motility. Proceedings of the National Academy of Sciences of the United States of America,79(14):4471~4475
Majors R E, Jerkovich A D, Mellors J S, Jorgenson J W.2003. The use of micrometer-sized particles inultrahigh pressure liquid chromatography. Lc Gc North America,21(7):600~610
Mallol R, Rodriguez M A, Heras M, Vinaixa M, Canellas N, Brezmes J, Plana N, Masana L, Correig X.2011. Surface fitting of2D diffusion-edited (1)H NMR spectroscopy data for the characterisation ofhuman plasma lipoproteins. Metabolomics,7(4):572~582
Maniara G, Rajamoorthi K, Rajan S, Stockton G W.1998. Method performance and validation forquantitative analysis by H-1and P-31NMR spectroscopy. Applications to analytical standards andagricultural chemicals. Analytical chemistry,70(23):4921~4928
Martinez-Borra J, Lopez-Larrea C, Gonzalez S, Fuentes D, Dieguez A, Deschamps E M, Perez-Pariente JM, Lopez-Vazquez A, de Francisco R, Rodrigo L.2002. High serum tumor necrosis factor-alpha levelsare associated with lack of response to infliximab in fistulizing Crohn's disease. American Journal ofGastroenterology,97(9):2350~2356
Martinez-Ceron M C, Marani M M, Taules M, Etcheverrigaray M, Albericio F, Cascone O, Camperi S A.2011. Affinity Chromatography Based on a Combinatorial Strategy for rErythropoietin Purification.Acs Combinatorial Science,13(3):251~258
Matsumoto M, Nomura T, Momose K, Ikeda Y, Kondou Y, Akiho H, Togami J, Kimura Y, Okada M,Yamaguchi T.1996. Inactivation of a novel neuropeptide Y peptide YY receptor gene in primatespecies. Journal of Biological Chemistry,271(44):27217~27220
Mentlein R, Dahms P, Grandt D, Kruger R.1993. Proteolytic processing of neuropeptide-Y and peptide-YYby dipeptidyl peptidase-IV. Regulatory Peptides,49(2):133~144
Michel M C, Beck-Sickinger A, Cox H, Doods H N, Herzog H, Larhammar D, Quirion R, Schwartz T,Westfall T.1998. XVI. International Union of Pharmacology recommendations for the nomenclatureof neuropeptide Y, peptide YY, and pancreatic polypeptide receptors. Pharmacological Reviews,50(1):143~150
Morley J E., Levine A S, Grace M, Kneip J.1985. Peptide YY (PYY), a potent orexigenic agent. Brainresearch,341(1):200~203
Naruse S, Kitagawa M, Ishiguro H, Hayakawa T.2002. Feedback regulation of pancreatic secretion bypeptide YY. Peptides,23(2):359~365
Naslund E, Gryback P, Hellstrom P M, Jacobsson H, Holst J J, Theodorsson E, Backman L.1997.Gastrointestinal hormones and gastric emptying20years after jejunoileal bypass for massive obesity.International Journal of Obesity,21(5):387~392
Naslund E, Bogefors J, Skogar S, Gryback P, Jacobsson H, Holst J J, Hellstrom P M.1999. GLP-1slowssolid gastric emptying and inhibits insulin, glucagon, and PYY release in humans. American Journalof Physiology-Regulatory Integrative and Comparative Physiology,277(3): R910~R916
Neary N M., Small C J, Druce M R, Park A J, Ellis S M, Semjonous N M, Dakin C L, Filipsson K, Wang F,Kent A S, Frost G S, Ghatei M A, Bloom S R.2005. Peptide YY3-36and glucagon-likepeptide-1(7-36) inhibit food intake additively. Endocrinology,146(12):5120~5127
Nilsson O, Bilchik A J, Goldenring J R, Ballantyne G H, Adrian T E, Modlin I M.1991. Distribution andimmunocytochemical colocalization of peptide YY and enteroglucagon in endocrine cells of the rabbitcolon. Endocrinology,129(1):139~148
Nonaka N, Shioda S, Niehoff M L, Banks W A.2003. Characterization of blood-brain barrier permeabilityto PYY3-36in the mouse. Journal of Pharmacology and Experimental Therapeutics,306(3):948~953
Ockander L, Hedenbro J L, Rehfeld J F, Sjolund K.2003. Jejunoileal bypass changes the duodenalcholecystokinin and somatostatin cell density. Obesity Surgery,13(4):584~590
Onaga T, Zabielski R, Kato S.2002. Multiple regulation of peptide YY secretion in the digestive tract.Peptides,23(2):279~290
Ortner K, Buchberger W.2008. Determination of sialic acids released from glycoproteins using capillaryzone electrophoresis/electrospray ionization mass spectrometry. Electrophoresis,29(10):2233~2237
Pilichiewicz A N, Little T J, Brennan I M, Meyer J H, Wishart J M, Otto B, Horowitz M, Feinle-Bisset C.2006. Effects of load, and duration, of duodenal lipid on antropyloroduodenal motility, plasma CCKand PYY, and energy intake in healthy men. American Journal of Physiology-Regulatory Integrativeand Comparative Physiology,290(3): R668~R677
Pita J, Rado-Peralta S, Gavela-Perez T, Aragon I, Barrios V, Rovira A, Argente J, Soriano-Guillen L.2011.Plasma kisspeptin levels are elevated in cord blood and present sexual dimorphism in the adultpopulation: Relation with leptin, gonadotropins and anthropometrical data. Peptides,32(5):983~988
Playford R J, Domin J, Beacham J, Parmar K B, Tatemoto K, Bloom S R, Calam J.1990. Preliminaryreport: role of peptide YY in defence against diarrhoea. Lancet,335(8705):1555~1557
Playford R J, Mehta S, Upton P, Rentch R, Moss S, Calam J, Bloom S, Payne N, Ghatei M, Edwards R,Unwin R.1995. Effect of peptide YY on human renal-function. American Journal ofPhysiology-Renal Physiology,268(4): F754~F759
Quin J A, Sgambati S A, Goldenring J R, Basson M D, Fielding L P, Modlin I M, Ballantyne G H.1995.PYY inhibition of VIP-stimulated ion-transport in the rabbit distal ileum. Journal of Surgical Research,58(1):111~115
Rice R H, Means G E, Brown W D.1977. Stabilization of bovine trypsin by reductive methylation.Biochimica et biophysica acta,492(2):316~321
Roth C L, Gebhardt U, Muller H L.2011. Appetite-Regulating Hormone Changes in Patients WithCraniopharyngioma. Obesity,19(1):36~42
Rudnicki M, Rigel D F, McFadden D W.1991. Vagal cooling blocks circulating neuropeptide Y (NPY),peptide YY (PYY), and pancreatic polypeptide (PP) release. The Journal of surgical research,51(1):40~45
Rudnicki M, Kuvshinoff B W, McFadden D W.1992. Extrinsic neural contribution to ileal peptide YY(PYY) release. Journal of Surgical Research,52(6):591~595
Saria A, Beubler E.1985. Neuropeptide Y (NPY) and peptide YY (PYY) inhibit prostaglandinE2-induced intestinal fluid and electrolyte secretion in the rat jejunum in vivo. European journal ofpharmacology,119(1-2):47~52
Savage A P, Adrian T E, Carolan G, Chatterjee V K.1987. Effects of peptide YY (PYY) on mouth tocaecum intestinal transit time and on the rate of gastric emptying in healthy volunteers. Gut,28(2):166~170
Sbrana E, Paladini A, Bramanti E, Spinetti M C, Raspi G.2004. Quantification of reduced glutathione andcysteine in human immunodeficiency virus-infected patients. Electrophoresis,25(10-11):1522~1529
Schmidt A, Karas M, Dulcks T.2003. Effect of different solution flow rates on analyte ion signals innano-ESI MS, or: When does ESI turn into nano-ESI? Journal of the American Society for MassSpectrometry,14(5):492~500
Shimura K, Hoshino M, Kamiya K, Katoh K, Hisada S, Matsumoto H, Kasai K.2002. Immunoassay ofserum alpha(1)-antitrypsin by affinity-probe capillary isoelectric focusing using a fluorescence-labeledrecombinant antibody fragment. Electrophoresis,23(6):909~917
Sileno A P, Brandt G C, Spann B M, Quay S C.2006. Lower mean weight after14days intravenousadministration peptide YY3-36(PYY3-36) in rabbits. International Journal of Obesity,30(1):68~72
Siskos A P, Katsila T, Balafas E, Kostomitsopoulos N, Tamvakopoulos C.2009. Simultaneous absolutequantification of the glucose-dependent insulinotropic polypeptides GIP(1-42) and GIP(3-42) inmouse plasma by LC/ESI-MS/MS: preclinical evaluation of DP-IV inhibitors. Journal of ProteomeResearch,8(7):3487~3496
Sloan J H, Ackermann B J, O'Dell M, Bowsher R R, Dean R A, Konrad R J.2011. Development of a novelradioimmunoassay to detect autoantibodies to amyloid beta peptides in the presence of a cross-reactivetherapeutic antibody. Journal of pharmaceutical and biomedical analysis,56(5):1029~1034
Sobhi H R., Vatansever B, Wortmann A, Grouzmann E, Rochat B.2011. Generic approach for the sensitiveabsolute quantification of large undigested peptides in plasma using a particular liquidchromatography-mass spectrometry setup. Journal of chromatography A,1218(47):8536~8543
Spiller R C, Trotman I F, Adrian T E, Bloom S R, Misiewicz J J, Silk D B.1988. Further characterisation ofthe 'ileal brake' reflex in man--effect of ileal infusion of partial digests of fat, protein, and starch onjejunal motility and release of neurotensin, enteroglucagon, and peptide YY. Gut,29(8):1042~1051
Spiller R C, Trotman I F, Higgins B E, Ghatei M A, Grimble G K, Lee Y C, Bloom S R, Misiewicz J J, SilkD B.1984. The ileal brake--inhibition of jejunal motility after ileal fat perfusion in man. Gut,25(4):365~374
Stanley S, Wynne K, Bloom S.2004. Gastrointestinal satiety signals-III. Glucagon-like peptide1,oxyntomodulin, peptide YY, and pancreatic polypeptide. American Journal ofPhysiology-Gastrointestinal and Liver Physiology,286(5): G693~G697
Staub A, Rudaz S, Saugy M, Veuthry J L, Schappler J.2010. Analysis of hemoglobin-based oxygen carriersby CE-UV/Vis and CE-ESI-TOF/MS. Electrophoresis,31(7):1241~1247
Stock S, Leichner P, Wong A C K, Ghatei M A, Kieffer T J, Bloom S R, Chanoine J P.2005. Ghrelin,peptide YY, glucose-dependent insulinotropic polypeptide, and hunger responses to a mixed meal inanorexic, obese, and control female adolescents. Journal of Clinical Endocrinology&Metabolism,90(4):2161~2168
Strader A D, Vahl T P, Jandacek R J, Woods S C, D'Alessio D A, Seeley R J.2005. Weight loss throughileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats.American Journal of Physiology-Endocrinology and Metabolism,288(2): E447~E453
Suzuki S, Ramos E J B, Goncalves C G, Chen C, Meguid M M.2005. Changes in GI hormones and theireffect on gastric emptying and transit times after Roux-en-Y gastric bypass in rat model. Surgery,138(2):283~290
Symersky T, Biemond I, Frolich M, Masclee A A M.2005. Effect of peptide YY on pancreatico-biliarysecretion in humans. Scandinavian journal of gastroenterology,40(8):944~949
Tachi T, Hase T, Okamoto Y, Kaji N, Arima T, Matsumoto H, Kondo M, Tokeshi M, Hasegawa Y, Baba Y.2011. A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarizationimmunoassay. Analytical and Bioanalytical Chemistry,401(7):2301~2305
Tatemoto K, Mutt V.1980. Isolation of two novel candidate hormones using a chemical method for findingnaturally occurring polypeptides. Nature,285(5764):417~418
Tatemoto K.1982a. Isolation and characterization of peptide YY (PYY), a candidate gut hormone thatinhibits pancreatic exocrine secretion. Proceedings of the National Academy of Sciences of the UnitedStates of America,79(8):2514~2518
Tatemoto K, Carlquist M, Mutt V.1982b. Neuropeptide Y--a novel brain peptide with structural similaritiesto peptide YY and pancreatic polypeptide. Nature,296(5858):659~660
Tatemoto K, Nakano I, Makk G, Angwin P, Mann M, Schilling J, Go V L.1988. Isolation and primarystructure of human peptide YY. Biochemical and biophysical research communications,157(2):713~717
Tishchenko G, Hodrova B, Simunek J, Bleha M.2003. Nickel and copper complexes of a chelatingmethacrylate sorbent in the purification of chitinases and specific immunoglobulin G1by immobilizedmetal ion affinity chromatography. Journal of Chromatography A,983(1~2):125~132
Tseng W W, Liu C D.2002. Peptide YY and cancer: current findings and potential clinical applications.Peptides,23(2):389~395
Tsiafoulis C G, Exarchou V, Tziova P P, Bairaktari E, Gerothanassis I P, Trogains A N.2011. A new methodfor the determination of free L-carnitine in serum samples based on high field single quantumcoherence filtering (1)H-NMR spectroscopy. Analytical and Bioanalytical Chemistry,399(6):2285~2294
Ukkola O H, Puurunen V P, Piira O P, Niva J T, Lepojarvi E S, Tulppo M P, Huikuri H V.2011. High serumfasting peptide YY (3-36) is associated with obesity-associated insulin resistance and type2diabetes.Regulatory Peptides,170(1-3):38~42
Unwin R D, Griffiths J R, Leverentz M K, Grallert A, Hagan I M, Whetton A D.2005. Multiple reactionmonitoring to identify sites of protein phosphorylation with high sensitivity. Molecular&CellularProteomics,4(8):1134~1144
Van Citters G W, Lin H C.1999. The ileal brake: a fifteen-year progress report. Current gastroenterologyreports,1(5):404~409
Van den Broek I, Sparidans R W, Schellens, J H M, Beijnen J H.2007. Enzymatic digestion as a tool for theLC-MS/MS quantification of large peptides in biological matrices: Measurement of chymotrypticfragments from the HIV-1fusion inhibitor enfuvirtide and its metabolite M-20in human plasma.Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences,854(1-2):245~259
Vera F, Zenuto R R, Antenucci C D, Busso J M, Marin R H.2011. Validation of a Radioimmunoassay forMeasuring Testosterone Concentrations in Plasma Samples of the Subterranean Rodent Ctenomystalarum: Outstandingly Elevated Levels in the Wild and the Effect of Captivity. Journal ofExperimental Zoology Part a-Ecological Genetics and Physiology,315A(9):572~583
Warner M R, Senanayake P D, Ferrario C M.1991. Sympathetic stimulation-evoked overflow ofnorepinephrine and neuropeptide Y from the heart. Circulation research,69(2):455~465
Wilbert S M, Engrissei G, Yau E K, Grainger D J, Tatalick L, Axworthy D B.2000. Quantitative analysis ofa synthetic peptide, NR58-3.14.3, in serum by LC-MS with inclusion of a diastereomer as internalstandard. Analytical Biochemistry,278(1):14~21
Wolf-Yadlin A, Hautaniemi S, Lauffenburger D A, White F M.2007. Multiple reaction monitoring forrobust quantitative proteomic analysis of cellular signaling networks. Proceedings of the NationalAcademy of Sciences of the United States of America,104(14):5860~5865
Wu G Q, Wang S L, Wang X Y, Li X F, Deng X P, Shen Z L, Xi T.2011. Determination of a newantibacterial peptide S-thanatin in rat plasma by an indirected-ELISA. Peptides,32(7):1484~1487
Wu N J, Collins D C, Lippert J A, Xiang Y Q, Lee M L.2000. Ultrahigh pressure liquidchromatography/time-of-flight mass spectrometry for fast separations. Journal of MicrocolumnSeparations,12(8):462~469
Wu S T, Ouyang Z, Olah T V, Jemal M.2011. A strategy for liquid chromatography/tandem massspectrometry based quantitation of pegylated protein drugs in plasma using plasma proteinprecipitation with water-miscible organic solvents and subsequent trypsin digestion to generatesurrogate peptides for detection. Rapid Communications in Mass Spectrometry,25(2):281~290
Xu K, Liu L N, Saad O M, Baudys J, Williams L, Leipold D, Shen B, Raab H, Junutula J R, Kim A, Kaur S.2011. Characterization of intact antibody-drug conjugates from plasma/serum in vivo by affinitycapture capillary liquid chromatography-mass spectrometry. Analytical Biochemistry,412(1):56~66
Yamamoto E, Yamamichi S, Choi-Miura, N H, Hasumi K.2010. The cyclopentapeptide plactin enhancescellular binding and autoactivation of the serine protease plasma hyaluronan-binding protein.Thrombosis Research,126(5):406~413
Zhang C, Rodriguez C, Circu M L, Aw T Y, Feng J.2011. S-glutathionyl quantification in the attomolerange using glutaredoxin-3-catalyzed cysteine derivatization and capillary gel electrophoresis withlaser-induced fluorescence detection. Analytical and Bioanalytical Chemistry,401(7):2165~2175
Zhang G D, Zhang Y Z, Fast D M, Lin Z S, Steenwyk R.2011. Ultra sensitive quantitation of endogenousoxytocin in rat and human plasma using a two-dimensional liquid chromatography-tandem massspectrometry assay. Analytical Biochemistry,416(1):45~52
Zhang L, Bijker M S, Herzog H.2011. The neuropeptide Y system: Pathophysiological and therapeuticimplications in obesity and cancer. Pharmacology&Therapeutics,131(1):91~113
Zhang T, Brubaker P L, Thompson J C, Greely G H.1993. Characterization of peptide-YY release inresponse to intracolonic infusion of amino-acids. Endocrinology,132(5):2205~2205
Zhang T, Uchida T, Gomez G, Liuis F, Thompson J C, Greeley G H.1993. Neural regulation of peptide-YYsecretion. Regulatory Peptides,48(3):321~328
Zheng C, Zhang S C, Ragg S, Raftery D, Vitek O.2011. Identification and quantification of metabolites in(1)H NMR spectra by Bayesian model selection. Bioinformatics,27(12):1637~1644
Zuliani G, Galvani M., Maggio M, Volpato S, Bandinelli S, Corsi A M, Lauretani F, Cherubini A, GuralnikJ M, Fellin R, Ferrucci L.2010. Plasma soluble gp130levels are increased in older subjects withmetabolic syndrome. The role of insulin resistance. Atherosclerosis,213(1):319~324
Adrian T E, Bacarese-Hamilton A J, Allen J M, Tatemoto K, Ferri G L, Polak J M, Bloom S R.1982.Distribution of PYY in the porcine and human gastrointestinal tract. Regulatory Peptides,4(6):355
Adrian T E, Savage A P, Sagor G R, Allen J M, Bacarese-Hamilton A J, Tatemoto K, polak J M, Bloom S R.1985. Effect of peptide YY on gastric, pancreatic, and biliary function in humans. Gastroenterology,89(3):494~499
Adrian T E, Sagor G R, Savage A P, Bacarese-Hamilton A J, Hall G M, Bloom S R.1986a. Peptide YYkinetics and effects on blood pressure and circulating pancreatic and gastrointestinal hormones andmetabolites in man. The Journal of clinical endocrinology and metabolism,63(4):803~807
Adrian T E, Savage A P, Bacarese-Hamilton A J, Wolfe K, Besterman H S, Bloom S R.1986b. PeptideYY abnormalities in gastrointestinal diseases. Gastroenterology,90(2):379~384
Adrian T E, Ballantyne G H, Longo W E, Bilchik A J, Graham S, Basson M D, Tierney R P, Modlin I M.1993. Deoxycholate is an important releaser of peptide-YY and enteroglucagon from the human colon.Gut,34(9):1219~1224
Ali-Rachedi A, Varndell I M, Adrian T E, Gapp D A, Van Noorden S, Bloom S R, Polak J M.1984. PeptideYY (PYY) immunoreactivity is co-stored with glucagon-related immunoreactants in endocrine cells ofthe gut and pancreas. Histochemistry,80(5):487~491
Anderson D S, Kirchner M, Kellogg M, Kalish L A, Jeong, J Y, Vanasse G, Berliner N, Fleming M D,Steen H.2011. Design and validation of a high-throughput matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry method for quantification of Hepcidin in human plasma. Analyticalchemistry,83(21):8357~8362
Anderson N L., Anderson N G, Haines L R, Hardie D B, Olafson R W, Pearson T W.2004. Massspectrometric quantitation of peptides and proteins using stable isotope standards and capture byanti-peptide antibodies (SISCAPA). Journal of Proteome Research,3(2):235~244
Andren P E, Caprioli R M.1999. Determination of extracellular release of neurotensin in discrete rat brainregions utilizing in vivo microdialysis/electrospray mass spectrometry. Brain research,845(2):123~129
Aponte G W, Fink A S, Meyer J H, Tatemoto K, Taylor I L.1985. Regional distribution and release ofpeptide YY with fatty acids of different chain length. The American journal of physiology,249(6Pt1):G745~750
Aristoteli L P, Molloy M P, Baker M S.2007. Evaluation of endogenous plasma peptide extraction methodsfor mass spectrometric biomarker discovery. Journal of Proteome Research,6(2):571~581
Armstrong D N, Krenz H K, Modlin I M, Ballantyne G H.1993. Bile-salt inhibition of motility in theisolated perfused rabbit terminal ileum. Gut,34(4):483~488
Aslan K, Grell T A J.2011. Rapid and sensitive detection of Troponin I in human whole blood samples byusing silver nanoparticle films and microwave heating clinical chemistry,57(5):746~752
Bagshaw R D, Callahan J W, Mahuran D J.2000. Desalting of in-gel-digested protein sample withmini-C18columns for matrix-assisted laser desorption ionization time of flight peptide massfingerprinting. Analytical Biochemistry,284(2):432~435
Ballantyne G H, Longo W E, Savoca P E, Adrian T E, Vukasin A P, Bilchik A J, Sussman J, Modlin I M.1989. Deoxycholate-stimulated release of peptide YY from the isolated perfused rabbit left colon. TheAmerican journal of physiology,257(5Pt1): G715~724
Ballantyne G H, Goldenring J R, Fleming F X, Rush S, Flint J S, Fielding L P, Binder H J, Modlin I M.1993a. Inhibition of VIP-stimulated ion-transport by a novel Y-receptor phenotype in rabbit distalcolon. American Journal of Physiology,264(5): G848~G854
Ballantyne G H, Goldenring J R, Savoca P E, Kranz H K, Adrian T E, Bilchik A J, Modlin I M.1993b.Cyclic AMP-mediated release of peptide-YY(PYY) from the isolated perfused rabbit distal colon.Regulatory Peptides,47(2):117~126
Ballantyne G H, A. Gumbs, Modlin I M.2005. Changes in insulin resistance following bariatric surgeryand the adipoinsular axis: Role of the adipocytokines, leptin, adiponectin and resistin. Obesity Surgery,15(5):692~699
Ballantyne G H.2006. Peptide YY(1-36) and peptide YY(3-36): Part I. Distribution, release and actions.Obesity Surgery,16(5):651~658
Barnidge D R, Goodmanson M K, Klee G G, Muddiman D C.2004. Absolute quantification of the modelbiomarker prostate-specific antigen in serum by LC-MS/MS using protein cleavage and isotopedilution mass spectrometry. Journal of Proteome Research,3(3):644~652
Bartolome M A, Borque M, Martinez-Sarmiento J, Aparicio E, Hernandez C, Cabrerizo L,Fernandez-Represa J A.2002. Peptide YY secretion in morbidly obese patients before and aftervertical banded gastroplasty. Obesity Surgery,12(3):324~327
Batterham R L, Cowley M A, Small C J, Herzog H, Cohen M A, Dakin C L, Wren A M, Brynes A E, LowM J, Ghatei M A, Cone R D, Bloom S R.2002. Gut hormone PYY3-36physiologically inhibits foodintake. Nature,418(6898):650~654
Batterham R L, Cohen M A, Ellis S M, Le Roux C W, Withers G J, Frost G S, Ghatei M A, Bloom S R.2003. Inhibition of food intake in obese subjects by peptide YY3-36. New England Journal ofMedicine,349(10):941~948
Beavis R C, Chaudhary T, Chait B T.1992. Alpha-cyano-4-hydroxycinnamic acid as a matrix formatrix-assited laser desorption mass-spectrometry. Organic Mass Spectrometry,27(2):156~158
Becher F, Pruvost A, Clement G, Tabet J C, Ezan E.2006. Quantification of small therapeutic proteins inplasma by liquid chromatography-Tandem mass spectrometry: Application to an elastase inhibitorEPI-hNE4. Analytical Chemistry,78(7):2306~2313
Beck E J, Tapsell L C, Batterham M J, Tosh S A, Huang X F.2009. Increases in peptide Y-Y levelsfollowing oat beta-glucan ingestion are dose-dependent in overweight adults. Nutrition Research,29(10):705~709
Berglund M M, Hipskind P A, Gehlert D R.2003. Recent developments in our understanding of thephysiological role of PP-fold peptide receptor subtypes. Experimental Biology and Medicine,228(3):217~244
Blundell T L, Pitts J E, Tickle I J, Wood S P, Wu C W.1981. X-ray analysis (1.4-A resolution) of avianpancreatic polypeptide: Small globular protein hormone. Proceedings of the National Academy ofSciences of the United States of America,78(7):4175~4179
Bornsen K O.2000. Influence of salts, buffers, detergents, solvents, and matrices on MALDI-MS proteinanalysis in complex mixtures. Methods in molecular biology,146:387~404
Bottcher G, Alumets J, Hakanson R, Sundler F.1986. Co-existence of glicentin and peptide YY incolorectal L-cells in cat and man. An electron microscopic study. Regulatory Peptides,13(3-4):283~291
Bridges T E, Marino V.1987. A rapid and sensitive method for the identification and quantitation ofsub-picomolar amounts of neurohypophysial peptides. Life sciences,41(26):2815~2822
Bronsky J., Nedvidkova J, Krasnicanova H, Vesela M, Schmidtova J, Koutek J, Kellermayer R, Chada M,Kabelka Z, Hrdlicka M, Nevoral J, Prusa R.2011. Changes of Orexin A Plasma Levels in Girls withAnorexia Nervosa During Eight Weeks of Realimentation. International Journal of Eating Disorders,44(6):547~552
Calderon-Santiago M, Mata-Granados J M, Priego-Capote F, Quesada-Gomez J M, De Castro M D L.2011.Analytical platform for verification and quantitation of target peptides in human serum: Application tocathelicidin. Analytical Biochemistry,415(1):39~45
Chen J L, Huang Y J, Pan C H, Hu C W, Chao M R.2011. Determination of urinary malondialdehyde byisotope dilution LC-MS/MS with automated solid-phase extraction: A cautionary note onderivatization optimization. Free radical biology and medicine,51(9):1823~1829
Clarke N J, Naylor S.2002. Capillary isoelectric focusing-mass spectrometry: analysis of protein mixturesfrom human body fluids. Biomedical Chromatography,16(4):287~297
Cool D R, DeBrosse D.2003. Extraction of oxytocin and arginine-vasopressin from serum and plasma forradioimmunoassay and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry.Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences,792(2):375~380
Corona G, Elia C, Casetta B, Frustaci S, Toffoli G.2011. High-throughput plasma docetaxel quantificationby liquid chromatography-tandem mass spectrometry. Clinica Chimica Acta,412(3-4):358~364
Cowles C L, Zhu X.2011. Sensitive detection of cardiac biomarker using ZnS nanoparticles as novel signaltransducers. Biosensors&bioelectronics,30(1):342~346
Cox H M, Cuthbert A W, Hakanson R, Wahlestedt C.1988. The effect of neuropeptide Y and peptide YYon electrogenic ion transport in rat intestinal epithelia. The Journal of physiology,398:65~80
Cuche G, Cuber J C, Malbert C H.2000. Ileal short-chain fatty acids inhibit gastric motility by a humoralpathway. American Journal of Physiology-Gastrointestinal and Liver Physiology,279(5): G925~G930
Dams R, Huestis M A, Lambert W E, Murphy C M.2003. Matrix effect in bio-analysis of illicit drugs withLC-MS/MS: Influence of ionization type, sample preparation, and biofluid. Journal of the AmericanSociety for Mass Spectrometry,14(11):1290~1294
De Hoog C L, Mann M.2004. Proteomics. Annual Review of Genomics and Human Genetics,5:267~293
Deacon C F.2004. Circulation and degradation of GIP and GLP-1. Hormone and metabolic research,36(11-12):761~765.
Degen L, Drewe J, Piccoli F, Grani K, Oesch S, Bunea R, Amato D M, Beglinger C.2007. Effect of CCK-1receptor blockade on ghrelin and PYY secretion in men. American Journal of Physiology-RegulatoryIntegrative and Comparative Physiology,292(4): R1391~R1399
Deng X Y, Guarita D R, Pedroso M R A, Kreiss C, Wood P G, Sved A F, Whitcomb D C.2001. PYYinhibits CCK-stimulated pancreatic secretion through the area postrema in unanesthetized rats.American Journal of Physiology-Regulatory Integrative and Comparative Physiology,281(2):R645~R653
Domon B, Aebersold R.2006. Review-Mass spectrometry and protein analysis. Science,312(5771):212~217
Druce M R, Small C J, Bloom S R.2004. Minireview: Gut peptides regulating satiety. Endocrinology,145(6):2660~2665
Emotte C, Deglave F, Heudi O, Picard F, Kretz O.2012. Fast simultaneous quantitative analysis of FTY720and its metabolite FTY720-P in human blood by on-line solid phase extraction coupled with liquidchromatography-tandem mass spectrometry. Journal of pharmaceutical and biomedical analysis,58:102~112
Erve J C L, Beyer C E, Manzino L, Talaat R E.2009. Metabolite identification in rat brain microdialysatesby direct infusion nanoelectrospray ionization after desalting on a ZipTip and LTQ/Orbitrap massspectrometry. Rapid Communications in Mass Spectrometry,23(24):4003~4012
Feltrin K L, Little T J, Meyer J H, Horowitz M, Rades T, Wishart J, Feinle-Bisset C.2008. Comparativeeffects of intraduodenal infusions of lauric and oleic acids on antropyloroduodenal motility, plasmacholecystokinin and peptide YY, appetite, and energy intake in healthy men. American Journal ofClinical Nutrition,87(5):1181~1187
Ferri G L, Adrian T E, Allen J M, Soimero L, Cancallieri A, Yeats J C, Blank M, Polak J M, Bloom S R.1988. Intramural distribution of regulatory peptides in the sigmoid-recto-anal region of the human gut.Gut,29(6):762~768
Frankevich V, Zhang J, Dashtiev M, Zenobi R.2003. Production and fragmentation of multiply chargedions in 'electron-free' matrix-assisted laser desorption/ionization. Rapid Communications in MassSpectrometry,17(20):2343~2348
FuCheng X M, Chariot J, Castex N, Galmiche J P, Roze C.1997. Mechanisms of peptide YY releaseinduced by an intraduodenal meal in rats: Neural regulation by proximal gut. PflugersArchiv-European Journal of Physiology,433(5):571~579
Gehlert D R.1998. Multiple receptors for the pancreatic polypeptide (PP-fold) family: Physiologicalimplications. Proceedings of the Society for Experimental Biology and Medicine,218(1):7~22
Goda R, Masumoto H, Okazaki O, Sudo K.2008. Development of a pretreatment method for amyloidbeta-protein analysis based on the effect of acetic acid on the dissolution of plasma polypeptides.Biomedical Chromatography,22(11):1279~1287
Grandt D, Schimiczek M, Beglinger C, Layer P, Goebell H, Eysselein V E, Reeve J R.1994a.2molecular-forms of peptide YY(PYY) are abundant in human blood-characterization of aradioimmunoassay recognizing PYY-1-36and PYY-3-36. Regulatory Peptides,51(2):151~159
Grandt D, Schimiczek M, Struk K, Shively J, Eysselein V E, Goebell H, Reeve J R.1994b.Characterization of2forms of peptide-YY,PYY(1-36) and PYY(3-36), in the rabbit. Peptides,15(5):815~820
Greeley G H Jr, Hashimoto T, Lzukura M, Gomes G, Jeng J, Hill F L, Liuis F, Thompson J C.1989a. Acomparison of intraduodenally and intracolonically administered nutrients on the release ofpeptide-YY in the dog. Endocrinology,125(4):1761~1765
Greeley G H Jr, Jeng J, Gomes G, Hashimoto T, Hill F L, Kern K, Kurosky T, Chuo H F, Thompson J C.1989b. Evidence for regulation of peptide-YY release by the proximal gut. Endocrinology,124(3):1438~1443
Greening D W, Simpson R J.2010. A centrifugal ultrafiltration strategy for isolating the low-molecularweight (<=25K) component of human plasma proteome. Journal of Proteomics,73(3):637~648
Gregor P, Feng Y, Decarr L B, Cornfield L J, Mccaleb M L.1996. Molecular characterization of a secondmouse pancreatic polypeptide receptor and its inactivated human homologue. Journal of BiologicalChemistry,271(44):27776~27781
Grimes J H, O'Connell T M.2011. The application of micro-coil NMR probe technology to metabolomicsof urine and serum. Journal of Biomolecular NMR,49(3-4):297~305
Guo Y S, Singh P, Debouno J F, Thompson J C.1988. Effect of peptide YY on insulin release stimulated by2-deoxyglucose and neuropeptides in dogs. Pancreas,3(2):128~134
Guo Y S, Singh P, Draviam E, Greeley G H Jr, Thompson J C.1989. Peptide YY inhibits the insulinotropicaction of gastric inhibitory polypeptide. Gastroenterology,96(3):690~694
Hagan M M, Moss D E.1995. Effect of Peptide YY (PYY) in food-associated conflict. Physiology&Behavior,58(4):731~735
Halfinger B, Sarg B, Amann A, Hammerer-Lercher A, Lindner H.2011. Unmasking low-abundancepeptides from human blood plasma and serum samples by a simple and robust two-stepprecipitation/immunoaffinity enrichment method. Electrophoresis,32(13):1706~1714
Hanusch-Enserer U, Roden M.2005. News in gut-brain communication: a role of peptide YY (PYY) inhuman obesity and following bariatric surgery? European Journal of Clinical Investigation,35(7):425~430
Heinig K, Wirz T, Yuan M, Tingler M, Mylott W.2011. An improved LC-MS/MS method for thedetermination of taspoglutide in plasma and urine using orthogonal HILIC-RP column switching,ultra-performance LC separation and 'wrong-way-round' electrospray ionization. BiomedicalChromatography,25(11):1215~1221
Heisler T, Towfigh S, Simon N, McFadden D W.2000. Peptide YY and vitamin E inhibithormone-sensitive and-insensitive breast cancer cells. Journal of Surgical Research,91(1):9~14
Hernandez E J, Whitcomb D C, Vigna S R, Taylor I L.1994. Saturable binding of circulating Peptide YY inthe dorsal vagal complex of rats. American Journal of Physiology,266(3): G511~G516
Hosotani R, Inoue K, Kogire M, Tatemoto K, Mutt V, Suzuki T, Rayford P L, Tobe T.1989. Effect ofnatural peptide YY on pancreatic secretion and cholecystokinin release in conscious dogs. Digestivediseases and sciences34(3):468~473
Huang K J, Jing Q S, Wei C Y, W Y Y.2011. Spectrofluorimetric determination of glutathione in humanplasma by solid-phase extraction using graphene as adsorbent. Spectrochimica Acta Part a-Molecularand Biomolecular Spectroscopy,79(5):1860~1865
Janzen N, Sander S, Terhardt M, Steuerwald U, Peter M, Das A M, Sander J.2011. Rapid steroid hormonequantification for congenital adrenal hyperplasia (CAH) in dried blood spots using UPLC liquidchromatography-tandem mass spectrometry. Steroids,76(13):1437~1442
Jian W, Romm M V, Edom R W, Miller V P, Lamarr W A, Weng N D.2011. Evaluation of ahigh-throughput online solid phase extraction-tandem mass spectrometry system for in vivobioanalytical studies. Analytical chemistry,83(21):8259~8266
John H, John S, Forssmann W G.2008. Kinetic studies on aminopeptidase M-mediated degradation ofhuman hemorphin LVV-H7and its N-terminally truncated products. Journal of Peptide Science,14(7):797~803
Jorgensen T J D, Bojesen G, Rahbek-Nielsen H.1998. The proton affinities of seven matrix-assisted laserdesorption/ionization matrices correlated with the formation of multiply charged ions. European MassSpectrometry,4(1):39~45
Kalra B, Kumar A, Patel K, Patel A, Khosravi K.2010. Development of a second generation Inhibin BELISA. Journal of Immunological Methods,362(1-2):22~31
Kanatani A, Mashiko S, Murai N, Sugimoto N, Ito J, Fukuroda T, Fukami T, Morin N, MacNeil D J, Vander Ploeg L H T, Saga Y, Nishimura S, Ihara M.2000. Role of the Y1receptor in the regulation ofneuropeptide Y-mediated feeding: Comparison of wild-type, Y1receptor-deficient, and Y5receptor-deficient mice. Endocrinology,141(3):1011~1016
Kay R, Barton C, Ratcliffe L, Matharoo-Ball B, Brown P, Roberts J, Teale P, Creaser C.2008. Enrichmentof low molecular weight serum proteins using acetonitrile precipitation for mass spectrometry basedproteomic analysis. Rapid Communications in Mass Spectrometry,22(20):3255~3260
Kazanjian K K, Towfigh S, et al.(2003). Peptide YY exhibits a mitogenic effect on pancreatic cells whileimproving acute pancreatitis in vitro. Journal of Surgical Research114(1):95~99
Keil-Dlouha V, Zylber N, Imhoff J M, Tong N T, Keil B.1971. Proteolytic activity of pseudotrypsin.FEBS Letters,16(4):291~295
Keire, D. A., P. Mannon, McFadden D W.2000. Primary structures of PYY, Pro(34) PYY, and PYY-(3-36)confer different conformations and receptor selectivity. American Journal ofPhysiology-Gastrointestinal and Liver Physiology,279(1): G126~G131
Kim B J, Carlson O D, Jang H J, Elahi D, Berry C, Egan J M.2005. Peptide YY is secreted after oralglucose administration in a gender-specific manner. Journal of Clinical Endocrinology&Metabolism,90(12):6665~6671
Koch T R, Roddy D R, Carney J A, Go V L.1988. Peptide YY concentrations in normal ileum and colonand in idiopathic inflammatory bowel disease. Digestive diseases and sciences,33(10):1322~1328
Konturek S J, Pepera J, Zabielski K, Konturek P C, Pawlik T, Szlachcic A, Hahn E G.2003. Brain-gut axisin pancreatic secretion and appetite control. Journal of Physiology and Pharmacology,54(3):293~317
Koomen J M, Li D H, Xiao L C, Liu T C, Coombes K R, Abbruzzese J, Kobayashi R.2005. Direct tandemmass spectrometry reveals limitations in protein profiling experiments for plasma biomarker discovery.Journal of Proteome Research,4(3):972~981
Korner J, Bessler M, Cirilo L J, Conwell I M, Daud A, Restuccia N L, Wardlaw S L.2005. Effects ofRoux-en-Y gastric bypass surgery on fasting and postprandial concentrations of plasma ghrelin,peptide YY, and insulin. Journal of Clinical Endocrinology&Metabolism,90(1):359~365
Korner J, Inabnet W, Conwell I M, Taveras C, Daud A, Olivero-Rivera L, Restuccia N L, Bessler M.2006.Differential effects of gastric bypass and banding on circulating gut hormone and leptin levels. Obesity,14(9):1553~1561
Kuhn E, Wu J, Karl J, Liao H, Zolg W, Guild B.2004. Quantification of C-reactive protein in the serum ofpatients with rheumatoid arthritis using multiple reaction monitoring mass spectrometry andC-13-labeled peptide standards. Proteomics,4(4):1175~1186
Kukucka M A, Misra H P.1994. Determination of oxytocin in biological samples by isocratichigh-performance-liquid chromatography with coulometric detection using C-18solid-phaseextraction and polyclonal antibody-based immunoaffinity column purification. Journal ofChromatography B-Biomedical Applications,653(2):139~145
Kuroda Y, Yukinaga H, Kitano M, Noguchi T, Nemati M, Shibukawa A, Nakagawa T, Matsuzaki K.2005.On-line capillary isoelectric focusing-mass spectrometry for quantitative analysis of peptides andproteins. Journal of pharmaceutical and biomedical analysis,37(3):423~428
Lame M E, Chambers E E, Blatnik M.2011. Quantitation of amyloid beta peptides Aβ1–38, Aβ1–40, andAβ1–42in human cerebrospinal fluid by ultra-performance liquid chromatography–tandem massspectrometry. Analytical Biochemistry,419(2):133~139
Larhammar D.1996. Structural diversity of receptors for neuropeptide Y, peptide YY and pancreaticpolypeptide. Regulatory Peptides,65(3):165~174
Larsimont V, Bogaards M, Mulleneers P, Hochhaus G.1999. Analysis of leucine enkephalin byhigh-performance liquid chromatography using enzymatic derivatization by tyrosinase andelectrochemical or fluorescence detection. Journal of pharmaceutical and biomedical analysis,19(6):855~864
Le Roux C W, Batterham R L, Aylwin S J B, Patterson M, Borg C M, Wynne K J, Kent A, Vincent R P,Gardiner J, Ghatei M A, Bloom S R.2006. Attenuated peptide YY release in obese subjects isassociated with reduced satiety. Endocrinology,147(1):3~8
Li H Y, Rose M J, Holder J R, Wright M, Miranda L P, James C A.2011. Direct Quantitative Analysis of a20kDa PEGylated Human Calcitonin Gene Peptide Antagonist in Cynomolgus Monkey Serum UsingIn-Source CID and UPLC-MS/MS. Journal of the American Society for Mass Spectrometry,22(9):1660~1667.
Lin H C, Zhao X T, Wang L J, Wong H.1996. Fat-induced ileal brake in the dog depends on peptide YY.Gastroenterology,110(5):1491~1495
Lin T M, Chance R E.1974. Candidate hormones of the gut. VI. Bovine pancreatic polypeptide (BPP) andavian pancreatic polypeptide (APP). Gastroenterology,67(4):737~738
Lluis F, Gomez G, Fujimura M, Greeley G H Jr, Thompson J C.1987. Peptide YY inhibits nutrient-,hormonal-, and vagally-stimulated pancreatic exocrine secretion. Pancreas,2(4):454~462
Lluis F, Gomez G, Fujimura M, Greeley G H Jr, Thompson J C.1988. Peptide YY inhibits pancreaticsecretion by inhibiting cholecystokinin release in the dog. Gastroenterology,94(1):137~144
Longo W E, Ballantyne G H, Savoca P E, Adrian T E, Bilchik A J, Modlin I M.1991. Short-chain fatty acidrelease of peptide YY in the isolated rabbit distal colon. Scandinavian journal of gastroenterology,26(4):442~448
Lugari R, Dei Cas A, Ugolotti D, Barilli A L, Camellini C, Ganzerla G C, Luciani A, Salerni B,Mittenperger F, Nodari S, Gnudi A, Zandomeneghi R.2004. Glucagon-like peptide1(GLP-1)secretion and plasma dipeptidyl peptidase IV (DPP-IV) activity in morbidly obese patients undergoingbiliopancreatic diversion. Hormone and Metabolic Research,36(2):111~115
Lundberg J M, Tatemoto K.1982. Vascular effects of the peptides PYY and PHI: comparison with APPand VIP. European journal of pharmacology,83(1-2):143~146
Lundberg J M, Tatemoto K, TereniusL, Hellstrom P M, Mutt V, Hokfelt T, Hamberger B.1982.Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal bloodflow and motility. Proceedings of the National Academy of Sciences of the United States of America,79(14):4471~4475
Majors R E, Jerkovich A D, Mellors J S, Jorgenson J W.2003. The use of micrometer-sized particles inultrahigh pressure liquid chromatography. Lc Gc North America,21(7):600~610
Mallol R, Rodriguez M A, Heras M, Vinaixa M, Canellas N, Brezmes J, Plana N, Masana L, Correig X.2011. Surface fitting of2D diffusion-edited (1)H NMR spectroscopy data for the characterisation ofhuman plasma lipoproteins. Metabolomics,7(4):572~582
Maniara G, Rajamoorthi K, Rajan S, Stockton G W.1998. Method performance and validation forquantitative analysis by H-1and P-31NMR spectroscopy. Applications to analytical standards andagricultural chemicals. Analytical chemistry,70(23):4921~4928
Martinez-Borra J, Lopez-Larrea C, Gonzalez S, Fuentes D, Dieguez A, Deschamps E M, Perez-Pariente JM, Lopez-Vazquez A, de Francisco R, Rodrigo L.2002. High serum tumor necrosis factor-alpha levelsare associated with lack of response to infliximab in fistulizing Crohn's disease. American Journal ofGastroenterology,97(9):2350~2356
Martinez-Ceron M C, Marani M M, Taules M, Etcheverrigaray M, Albericio F, Cascone O, Camperi S A.2011. Affinity Chromatography Based on a Combinatorial Strategy for rErythropoietin Purification.Acs Combinatorial Science,13(3):251~258
Matsumoto M, Nomura T, Momose K, Ikeda Y, Kondou Y, Akiho H, Togami J, Kimura Y, Okada M,Yamaguchi T.1996. Inactivation of a novel neuropeptide Y peptide YY receptor gene in primatespecies. Journal of Biological Chemistry,271(44):27217~27220
Mentlein R, Dahms P, Grandt D, Kruger R.1993. Proteolytic processing of neuropeptide-Y and peptide-YYby dipeptidyl peptidase-IV. Regulatory Peptides,49(2):133~144
Michel M C, Beck-Sickinger A, Cox H, Doods H N, Herzog H, Larhammar D, Quirion R, Schwartz T,Westfall T.1998. XVI. International Union of Pharmacology recommendations for the nomenclatureof neuropeptide Y, peptide YY, and pancreatic polypeptide receptors. Pharmacological Reviews,50(1):143~150
Morley J E., Levine A S, Grace M, Kneip J.1985. Peptide YY (PYY), a potent orexigenic agent. Brainresearch,341(1):200~203
Naruse S, Kitagawa M, Ishiguro H, Hayakawa T.2002. Feedback regulation of pancreatic secretion bypeptide YY. Peptides,23(2):359~365
Naslund E, Gryback P, Hellstrom P M, Jacobsson H, Holst J J, Theodorsson E, Backman L.1997.Gastrointestinal hormones and gastric emptying20years after jejunoileal bypass for massive obesity.International Journal of Obesity,21(5):387~392
Naslund E, Bogefors J, Skogar S, Gryback P, Jacobsson H, Holst J J, Hellstrom P M.1999. GLP-1slowssolid gastric emptying and inhibits insulin, glucagon, and PYY release in humans. American Journalof Physiology-Regulatory Integrative and Comparative Physiology,277(3): R910~R916
Neary N M., Small C J, Druce M R, Park A J, Ellis S M, Semjonous N M, Dakin C L, Filipsson K, Wang F,Kent A S, Frost G S, Ghatei M A, Bloom S R.2005. Peptide YY3-36and glucagon-likepeptide-1(7-36) inhibit food intake additively. Endocrinology,146(12):5120~5127
Nilsson O, Bilchik A J, Goldenring J R, Ballantyne G H, Adrian T E, Modlin I M.1991. Distribution andimmunocytochemical colocalization of peptide YY and enteroglucagon in endocrine cells of the rabbitcolon. Endocrinology,129(1):139~148
Nonaka N, Shioda S, Niehoff M L, Banks W A.2003. Characterization of blood-brain barrier permeabilityto PYY3-36in the mouse. Journal of Pharmacology and Experimental Therapeutics,306(3):948~953
Ockander L, Hedenbro J L, Rehfeld J F, Sjolund K.2003. Jejunoileal bypass changes the duodenalcholecystokinin and somatostatin cell density. Obesity Surgery,13(4):584~590
Onaga T, Zabielski R, Kato S.2002. Multiple regulation of peptide YY secretion in the digestive tract.Peptides,23(2):279~290
Ortner K, Buchberger W.2008. Determination of sialic acids released from glycoproteins using capillaryzone electrophoresis/electrospray ionization mass spectrometry. Electrophoresis,29(10):2233~2237
Pilichiewicz A N, Little T J, Brennan I M, Meyer J H, Wishart J M, Otto B, Horowitz M, Feinle-Bisset C.2006. Effects of load, and duration, of duodenal lipid on antropyloroduodenal motility, plasma CCKand PYY, and energy intake in healthy men. American Journal of Physiology-Regulatory Integrativeand Comparative Physiology,290(3): R668~R677
Pita J, Rado-Peralta S, Gavela-Perez T, Aragon I, Barrios V, Rovira A, Argente J, Soriano-Guillen L.2011.Plasma kisspeptin levels are elevated in cord blood and present sexual dimorphism in the adultpopulation: Relation with leptin, gonadotropins and anthropometrical data. Peptides,32(5):983~988
Playford R J, Domin J, Beacham J, Parmar K B, Tatemoto K, Bloom S R, Calam J.1990. Preliminaryreport: role of peptide YY in defence against diarrhoea. Lancet,335(8705):1555~1557
Playford R J, Mehta S, Upton P, Rentch R, Moss S, Calam J, Bloom S, Payne N, Ghatei M, Edwards R,Unwin R.1995. Effect of peptide YY on human renal-function. American Journal ofPhysiology-Renal Physiology,268(4): F754~F759
Quin J A, Sgambati S A, Goldenring J R, Basson M D, Fielding L P, Modlin I M, Ballantyne G H.1995.PYY inhibition of VIP-stimulated ion-transport in the rabbit distal ileum. Journal of Surgical Research,58(1):111~115
Rice R H, Means G E, Brown W D.1977. Stabilization of bovine trypsin by reductive methylation.Biochimica et biophysica acta,492(2):316~321
Roth C L, Gebhardt U, Muller H L.2011. Appetite-Regulating Hormone Changes in Patients WithCraniopharyngioma. Obesity,19(1):36~42
Rudnicki M, Rigel D F, McFadden D W.1991. Vagal cooling blocks circulating neuropeptide Y (NPY),peptide YY (PYY), and pancreatic polypeptide (PP) release. The Journal of surgical research,51(1):40~45
Rudnicki M, Kuvshinoff B W, McFadden D W.1992. Extrinsic neural contribution to ileal peptide YY(PYY) release. Journal of Surgical Research,52(6):591~595
Saria A, Beubler E.1985. Neuropeptide Y (NPY) and peptide YY (PYY) inhibit prostaglandinE2-induced intestinal fluid and electrolyte secretion in the rat jejunum in vivo. European journal ofpharmacology,119(1-2):47~52
Savage A P, Adrian T E, Carolan G, Chatterjee V K.1987. Effects of peptide YY (PYY) on mouth tocaecum intestinal transit time and on the rate of gastric emptying in healthy volunteers. Gut,28(2):166~170
Sbrana E, Paladini A, Bramanti E, Spinetti M C, Raspi G.2004. Quantification of reduced glutathione andcysteine in human immunodeficiency virus-infected patients. Electrophoresis,25(10-11):1522~1529
Schmidt A, Karas M, Dulcks T.2003. Effect of different solution flow rates on analyte ion signals innano-ESI MS, or: When does ESI turn into nano-ESI? Journal of the American Society for MassSpectrometry,14(5):492~500
Shimura K, Hoshino M, Kamiya K, Katoh K, Hisada S, Matsumoto H, Kasai K.2002. Immunoassay ofserum alpha(1)-antitrypsin by affinity-probe capillary isoelectric focusing using a fluorescence-labeledrecombinant antibody fragment. Electrophoresis,23(6):909~917
Sileno A P, Brandt G C, Spann B M, Quay S C.2006. Lower mean weight after14days intravenousadministration peptide YY3-36(PYY3-36) in rabbits. International Journal of Obesity,30(1):68~72
Siskos A P, Katsila T, Balafas E, Kostomitsopoulos N, Tamvakopoulos C.2009. Simultaneous absolutequantification of the glucose-dependent insulinotropic polypeptides GIP(1-42) and GIP(3-42) inmouse plasma by LC/ESI-MS/MS: preclinical evaluation of DP-IV inhibitors. Journal of ProteomeResearch,8(7):3487~3496
Sloan J H, Ackermann B J, O'Dell M, Bowsher R R, Dean R A, Konrad R J.2011. Development of a novelradioimmunoassay to detect autoantibodies to amyloid beta peptides in the presence of a cross-reactivetherapeutic antibody. Journal of pharmaceutical and biomedical analysis,56(5):1029~1034
Sobhi H R., Vatansever B, Wortmann A, Grouzmann E, Rochat B.2011. Generic approach for the sensitiveabsolute quantification of large undigested peptides in plasma using a particular liquidchromatography-mass spectrometry setup. Journal of chromatography A,1218(47):8536~8543
Spiller R C, Trotman I F, Adrian T E, Bloom S R, Misiewicz J J, Silk D B.1988. Further characterisation ofthe 'ileal brake' reflex in man--effect of ileal infusion of partial digests of fat, protein, and starch onjejunal motility and release of neurotensin, enteroglucagon, and peptide YY. Gut,29(8):1042~1051
Spiller R C, Trotman I F, Higgins B E, Ghatei M A, Grimble G K, Lee Y C, Bloom S R, Misiewicz J J, SilkD B.1984. The ileal brake--inhibition of jejunal motility after ileal fat perfusion in man. Gut,25(4):365~374
Stanley S, Wynne K, Bloom S.2004. Gastrointestinal satiety signals-III. Glucagon-like peptide1,oxyntomodulin, peptide YY, and pancreatic polypeptide. American Journal ofPhysiology-Gastrointestinal and Liver Physiology,286(5): G693~G697
Staub A, Rudaz S, Saugy M, Veuthry J L, Schappler J.2010. Analysis of hemoglobin-based oxygen carriersby CE-UV/Vis and CE-ESI-TOF/MS. Electrophoresis,31(7):1241~1247
Stock S, Leichner P, Wong A C K, Ghatei M A, Kieffer T J, Bloom S R, Chanoine J P.2005. Ghrelin,peptide YY, glucose-dependent insulinotropic polypeptide, and hunger responses to a mixed meal inanorexic, obese, and control female adolescents. Journal of Clinical Endocrinology&Metabolism,90(4):2161~2168
Strader A D, Vahl T P, Jandacek R J, Woods S C, D'Alessio D A, Seeley R J.2005. Weight loss throughileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats.American Journal of Physiology-Endocrinology and Metabolism,288(2): E447~E453
Suzuki S, Ramos E J B, Goncalves C G, Chen C, Meguid M M.2005. Changes in GI hormones and theireffect on gastric emptying and transit times after Roux-en-Y gastric bypass in rat model. Surgery,138(2):283~290
Symersky T, Biemond I, Frolich M, Masclee A A M.2005. Effect of peptide YY on pancreatico-biliarysecretion in humans. Scandinavian journal of gastroenterology,40(8):944~949
Tachi T, Hase T, Okamoto Y, Kaji N, Arima T, Matsumoto H, Kondo M, Tokeshi M, Hasegawa Y, Baba Y.2011. A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarizationimmunoassay. Analytical and Bioanalytical Chemistry,401(7):2301~2305
Tatemoto K, Mutt V.1980. Isolation of two novel candidate hormones using a chemical method for findingnaturally occurring polypeptides. Nature,285(5764):417~418
Tatemoto K.1982a. Isolation and characterization of peptide YY (PYY), a candidate gut hormone thatinhibits pancreatic exocrine secretion. Proceedings of the National Academy of Sciences of the UnitedStates of America,79(8):2514~2518
Tatemoto K, Carlquist M, Mutt V.1982b. Neuropeptide Y--a novel brain peptide with structural similaritiesto peptide YY and pancreatic polypeptide. Nature,296(5858):659~660
Tatemoto K, Nakano I, Makk G, Angwin P, Mann M, Schilling J, Go V L.1988. Isolation and primarystructure of human peptide YY. Biochemical and biophysical research communications,157(2):713~717
Tishchenko G, Hodrova B, Simunek J, Bleha M.2003. Nickel and copper complexes of a chelatingmethacrylate sorbent in the purification of chitinases and specific immunoglobulin G1by immobilizedmetal ion affinity chromatography. Journal of Chromatography A,983(1~2):125~132
Tseng W W, Liu C D.2002. Peptide YY and cancer: current findings and potential clinical applications.Peptides,23(2):389~395
Tsiafoulis C G, Exarchou V, Tziova P P, Bairaktari E, Gerothanassis I P, Trogains A N.2011. A new methodfor the determination of free L-carnitine in serum samples based on high field single quantumcoherence filtering (1)H-NMR spectroscopy. Analytical and Bioanalytical Chemistry,399(6):2285~2294
Ukkola O H, Puurunen V P, Piira O P, Niva J T, Lepojarvi E S, Tulppo M P, Huikuri H V.2011. High serumfasting peptide YY (3-36) is associated with obesity-associated insulin resistance and type2diabetes.Regulatory Peptides,170(1-3):38~42
Unwin R D, Griffiths J R, Leverentz M K, Grallert A, Hagan I M, Whetton A D.2005. Multiple reactionmonitoring to identify sites of protein phosphorylation with high sensitivity. Molecular&CellularProteomics,4(8):1134~1144
Van Citters G W, Lin H C.1999. The ileal brake: a fifteen-year progress report. Current gastroenterologyreports,1(5):404~409
Van den Broek I, Sparidans R W, Schellens, J H M, Beijnen J H.2007. Enzymatic digestion as a tool for theLC-MS/MS quantification of large peptides in biological matrices: Measurement of chymotrypticfragments from the HIV-1fusion inhibitor enfuvirtide and its metabolite M-20in human plasma.Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences,854(1-2):245~259
Vera F, Zenuto R R, Antenucci C D, Busso J M, Marin R H.2011. Validation of a Radioimmunoassay forMeasuring Testosterone Concentrations in Plasma Samples of the Subterranean Rodent Ctenomystalarum: Outstandingly Elevated Levels in the Wild and the Effect of Captivity. Journal ofExperimental Zoology Part a-Ecological Genetics and Physiology,315A(9):572~583
Warner M R, Senanayake P D, Ferrario C M.1991. Sympathetic stimulation-evoked overflow ofnorepinephrine and neuropeptide Y from the heart. Circulation research,69(2):455~465
Wilbert S M, Engrissei G, Yau E K, Grainger D J, Tatalick L, Axworthy D B.2000. Quantitative analysis ofa synthetic peptide, NR58-3.14.3, in serum by LC-MS with inclusion of a diastereomer as internalstandard. Analytical Biochemistry,278(1):14~21
Wolf-Yadlin A, Hautaniemi S, Lauffenburger D A, White F M.2007. Multiple reaction monitoring forrobust quantitative proteomic analysis of cellular signaling networks. Proceedings of the NationalAcademy of Sciences of the United States of America,104(14):5860~5865
Wu G Q, Wang S L, Wang X Y, Li X F, Deng X P, Shen Z L, Xi T.2011. Determination of a newantibacterial peptide S-thanatin in rat plasma by an indirected-ELISA. Peptides,32(7):1484~1487
Wu N J, Collins D C, Lippert J A, Xiang Y Q, Lee M L.2000. Ultrahigh pressure liquidchromatography/time-of-flight mass spectrometry for fast separations. Journal of MicrocolumnSeparations,12(8):462~469
Wu S T, Ouyang Z, Olah T V, Jemal M.2011. A strategy for liquid chromatography/tandem massspectrometry based quantitation of pegylated protein drugs in plasma using plasma proteinprecipitation with water-miscible organic solvents and subsequent trypsin digestion to generatesurrogate peptides for detection. Rapid Communications in Mass Spectrometry,25(2):281~290
Xu K, Liu L N, Saad O M, Baudys J, Williams L, Leipold D, Shen B, Raab H, Junutula J R, Kim A, Kaur S.2011. Characterization of intact antibody-drug conjugates from plasma/serum in vivo by affinitycapture capillary liquid chromatography-mass spectrometry. Analytical Biochemistry,412(1):56~66
Yamamoto E, Yamamichi S, Choi-Miura, N H, Hasumi K.2010. The cyclopentapeptide plactin enhancescellular binding and autoactivation of the serine protease plasma hyaluronan-binding protein.Thrombosis Research,126(5):406~413
Zhang C, Rodriguez C, Circu M L, Aw T Y, Feng J.2011. S-glutathionyl quantification in the attomolerange using glutaredoxin-3-catalyzed cysteine derivatization and capillary gel electrophoresis withlaser-induced fluorescence detection. Analytical and Bioanalytical Chemistry,401(7):2165~2175
Zhang G D, Zhang Y Z, Fast D M, Lin Z S, Steenwyk R.2011. Ultra sensitive quantitation of endogenousoxytocin in rat and human plasma using a two-dimensional liquid chromatography-tandem massspectrometry assay. Analytical Biochemistry,416(1):45~52
Zhang L, Bijker M S, Herzog H.2011. The neuropeptide Y system: Pathophysiological and therapeuticimplications in obesity and cancer. Pharmacology&Therapeutics,131(1):91~113
Zhang T, Brubaker P L, Thompson J C, Greely G H.1993. Characterization of peptide-YY release inresponse to intracolonic infusion of amino-acids. Endocrinology,132(5):2205~2205
Zhang T, Uchida T, Gomez G, Liuis F, Thompson J C, Greeley G H.1993. Neural regulation of peptide-YYsecretion. Regulatory Peptides,48(3):321~328
Zheng C, Zhang S C, Ragg S, Raftery D, Vitek O.2011. Identification and quantification of metabolites in(1)H NMR spectra by Bayesian model selection. Bioinformatics,27(12):1637~1644
Zuliani G, Galvani M., Maggio M, Volpato S, Bandinelli S, Corsi A M, Lauretani F, Cherubini A, GuralnikJ M, Fellin R, Ferrucci L.2010. Plasma soluble gp130levels are increased in older subjects withmetabolic syndrome. The role of insulin resistance. Atherosclerosis,213(1):319~324
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |