紫锥菊、紫甘薯中活性成分高效制备技术及紫锥菊生物活性研究
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摘要
为进一步研究植物中化学成分及其生理活性,本论文对紫锥菊多种化学成分进行制备分离、活性筛选及紫甘薯中主要化学成分的制备分离,取得主要结果如下：
1、采用高速逆流色谱与制备液相色谱技术制备分离得到紫锥菊中13个高纯度化合物,经鉴定,3个为新化合物：2-O-异阿魏酰基酒石酸、2-O-咖啡酰基-3-O-异阿魏酰酒石酸、及2,3-O-双异阿魏酰基酒石酸。
2、采用高速逆流色谱技术从国产紫甘薯中分离制备得到4个化合物,经质谱、核磁共振、紫外等技术进行结构鉴定,分别为3-O-{6-O-(E)-咖啡酰-2-O-[6-O-(E)-对羟基苯甲酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-(β-D-吡喃葡糖苷)]芍药素、3-O-{6-O-(E)-咖啡酰-2-O-[6-O-(E)-阿魏酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-(β-D-吡喃葡糖苷)]芍药素、3-O-咖啡酰基奎宁酸,3,5-O-二咖啡酰基奎宁酸。
3、采用流感病毒神经氨酸酶抑制模型研究了紫锥菊单体化合物及不同规格提取物对流感病毒A/PR/8/34(H1N1)神经氨酸酶的抑制作用。结果表明,7个肉桂酸类化合物均显示出显著抑制作用,其中菊苣酸的抑制作用最强(IC50=5.68±0.19μg/mL),表明紫锥菊具有潜在的抗病毒活性。
4、采用磺酰罗丹明B染色法研究了紫锥菊单体化合物及不同规格提取物对人肝癌BEL-7402及人乳腺癌MCF-7细胞株的抑制作用。结果表明,肉桂酸类化合物及烷基酰胺单体化合物对肿瘤细胞抑制作用较弱,但烷基酰胺类混合物对两种肿瘤细胞的抑制作用较强,半抑制浓度IC50分别为0.05mg/mL、0.03mg/mL。
5、采用趋化因子受体拮抗剂筛选模型研究了紫锥菊单体化合物及不同规格提取物对趋化因子受体CXCR4、CCR5的拮抗作用。结果表明,各样品仅具有较弱的拮抗作用,表明紫锥菊对HIV病毒侵入宿主细胞时的抑制作用不明显。
6、研究了紫锥菊单体化合物及不同规格提取物对金黄色葡萄球菌、大肠杆菌、沙门氏菌、溶血性链球菌的抑制作用。结果表明各样品均无抑制作用。
7、对紫锥菊肉桂酸类化合物进行体外抗氧化作用及其构效关系研究,结果表明化合物抗氧化性强弱基本表现为：菊苣酸、2-O-咖啡酰基酒石酸、2-O-咖啡酰基-3-O-阿魏酰酒石酸、2,3-O-双异阿魏酰基酒石酸、2-O-异阿魏酰基酒石酸；体外抗氧化活性大小与咖啡酸苯环上的酚羟基位置、数目相关,其中对位酚羟基的活性最强,邻位羟基较弱；对DNA损伤均具有显著保护作用,但构效关系不明显。
8、建立了HPLC-PDA方法同时检测紫锥菊中7个肉桂酸及5个烷基酰胺类化合物的方法,通过梯度洗脱于60min内可在C18反相色谱柱上完全分离,样品预处理简单,可作为紫锥菊E.purpurea中活性成分的标准检测方法。
This study has two parts which one deals mainly with chemical, bioactive investigation and HPLC detection method on Echinacea purpurea (L.) Moench and another deals with chemical on Purple sweet potato, the main results are as follows:
1、Set up High-speed countercurrent chromatography and preparative HPLC methods to separate cinnamic acids and alkylamides in Echinacea purpurea, fifteen high-purity compounds were got from Echinacea purpurea and thirteen structures were identified by MS, NMR, UV, three compounds such as2-O-isoferuloyltartaric acid,2-O-caffeoyl-3-O-isoferuloyltartaric acid,2,3-di-O-isoferuloyltartaric acid were new compounds.
2、Set up a High-speed countercurrent chromatography mothed to separate four compounds from purple sweet potato, the structure of them identified by MS, NMR, UV, the resuls showed two of them were acylated anthocyanins and anther were chlorogenic acid and ischlorogenic acid A.
3、The model of inhibition influenza virus neuraminidase enzyme has been used to study the compounds in Echinacea purpurea on the neuraminidase of influenza virus A/PR/8/34(H1N1). The results showed that cinnamic acids have significant inhibitions, cinnamic acid compound3was the strongest and IC50values was5.68±0.19μg/mL. Suggesting that one pathway of antiviral activity of Echinacea purpurea was the inhibition of virus neuraminidase enzyme.
4、Sulfonyl rhodamine B staining method has been used to study the compounds of Echinacea purpurea on BEL-7402human hepatoma and MCF-7human breast cancer cell lines. The results showed that cinnamic acids and alkylamide compounds have weak inhibition on tumor cell, while the mixture of alkylamides were strong inhibition compare with purity compound, IC50values were0.05mg/mL and0.03mg/mL respectively.
5、The model of antagonism has been used to study the compounds of Echinacea purpurea on chemokine receptor CXCR4and CCR5. The results showed that all the samples only have a weak antagonism, suggesting it was not obviously that Echinacea against HIV in the infection of host cells.
6^Study the inhibition of compounds of Echinacea purpure on four kinds of bacteria. The results showed there were no inhibition on bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella and hemolytic streptococcus.
7、Chemical method have been used to study antioxidant activities of cinnamic acid derivatives of Echinacea purpure, the results showed that the antioxidant of compounds were as follows:Cichoric acid,2-caffeoyltartaric acid,2-caffeoyl-3-feruloyltartaric acid,2,3-diisferuloyltartaric acid,2-isferuloyltartaric acid; Structure-activity relationship showed:active groups of antioxidantactive was the phenolic hydroxyl on benzene of caffeic acide groups, and the bit phenolic hydroxyl was the strongest activity and adjacent was weak, but the protective effect of DNA damage does not exist this relationship, indicating that the protective effect has different action mechanism.
8、Simultaneous analysis7cinnamic acid compounds and5alkylamide compounds in Echinacea purpure by HPLC-PDA with gradient elution on a C18reverse phase column in60minutes without the pretreatment of samples, it was suitable as standard analytical method to detect the active ingredients in Echinacea purpure.
1、采用高速逆流色谱与制备液相色谱技术制备分离得到紫锥菊中13个高纯度化合物,经鉴定,3个为新化合物：2-O-异阿魏酰基酒石酸、2-O-咖啡酰基-3-O-异阿魏酰酒石酸、及2,3-O-双异阿魏酰基酒石酸。
2、采用高速逆流色谱技术从国产紫甘薯中分离制备得到4个化合物,经质谱、核磁共振、紫外等技术进行结构鉴定,分别为3-O-{6-O-(E)-咖啡酰-2-O-[6-O-(E)-对羟基苯甲酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-(β-D-吡喃葡糖苷)]芍药素、3-O-{6-O-(E)-咖啡酰-2-O-[6-O-(E)-阿魏酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-(β-D-吡喃葡糖苷)]芍药素、3-O-咖啡酰基奎宁酸,3,5-O-二咖啡酰基奎宁酸。
3、采用流感病毒神经氨酸酶抑制模型研究了紫锥菊单体化合物及不同规格提取物对流感病毒A/PR/8/34(H1N1)神经氨酸酶的抑制作用。结果表明,7个肉桂酸类化合物均显示出显著抑制作用,其中菊苣酸的抑制作用最强(IC50=5.68±0.19μg/mL),表明紫锥菊具有潜在的抗病毒活性。
4、采用磺酰罗丹明B染色法研究了紫锥菊单体化合物及不同规格提取物对人肝癌BEL-7402及人乳腺癌MCF-7细胞株的抑制作用。结果表明,肉桂酸类化合物及烷基酰胺单体化合物对肿瘤细胞抑制作用较弱,但烷基酰胺类混合物对两种肿瘤细胞的抑制作用较强,半抑制浓度IC50分别为0.05mg/mL、0.03mg/mL。
5、采用趋化因子受体拮抗剂筛选模型研究了紫锥菊单体化合物及不同规格提取物对趋化因子受体CXCR4、CCR5的拮抗作用。结果表明,各样品仅具有较弱的拮抗作用,表明紫锥菊对HIV病毒侵入宿主细胞时的抑制作用不明显。
6、研究了紫锥菊单体化合物及不同规格提取物对金黄色葡萄球菌、大肠杆菌、沙门氏菌、溶血性链球菌的抑制作用。结果表明各样品均无抑制作用。
7、对紫锥菊肉桂酸类化合物进行体外抗氧化作用及其构效关系研究,结果表明化合物抗氧化性强弱基本表现为：菊苣酸、2-O-咖啡酰基酒石酸、2-O-咖啡酰基-3-O-阿魏酰酒石酸、2,3-O-双异阿魏酰基酒石酸、2-O-异阿魏酰基酒石酸；体外抗氧化活性大小与咖啡酸苯环上的酚羟基位置、数目相关,其中对位酚羟基的活性最强,邻位羟基较弱；对DNA损伤均具有显著保护作用,但构效关系不明显。
8、建立了HPLC-PDA方法同时检测紫锥菊中7个肉桂酸及5个烷基酰胺类化合物的方法,通过梯度洗脱于60min内可在C18反相色谱柱上完全分离,样品预处理简单,可作为紫锥菊E.purpurea中活性成分的标准检测方法。
This study has two parts which one deals mainly with chemical, bioactive investigation and HPLC detection method on Echinacea purpurea (L.) Moench and another deals with chemical on Purple sweet potato, the main results are as follows:
1、Set up High-speed countercurrent chromatography and preparative HPLC methods to separate cinnamic acids and alkylamides in Echinacea purpurea, fifteen high-purity compounds were got from Echinacea purpurea and thirteen structures were identified by MS, NMR, UV, three compounds such as2-O-isoferuloyltartaric acid,2-O-caffeoyl-3-O-isoferuloyltartaric acid,2,3-di-O-isoferuloyltartaric acid were new compounds.
2、Set up a High-speed countercurrent chromatography mothed to separate four compounds from purple sweet potato, the structure of them identified by MS, NMR, UV, the resuls showed two of them were acylated anthocyanins and anther were chlorogenic acid and ischlorogenic acid A.
3、The model of inhibition influenza virus neuraminidase enzyme has been used to study the compounds in Echinacea purpurea on the neuraminidase of influenza virus A/PR/8/34(H1N1). The results showed that cinnamic acids have significant inhibitions, cinnamic acid compound3was the strongest and IC50values was5.68±0.19μg/mL. Suggesting that one pathway of antiviral activity of Echinacea purpurea was the inhibition of virus neuraminidase enzyme.
4、Sulfonyl rhodamine B staining method has been used to study the compounds of Echinacea purpurea on BEL-7402human hepatoma and MCF-7human breast cancer cell lines. The results showed that cinnamic acids and alkylamide compounds have weak inhibition on tumor cell, while the mixture of alkylamides were strong inhibition compare with purity compound, IC50values were0.05mg/mL and0.03mg/mL respectively.
5、The model of antagonism has been used to study the compounds of Echinacea purpurea on chemokine receptor CXCR4and CCR5. The results showed that all the samples only have a weak antagonism, suggesting it was not obviously that Echinacea against HIV in the infection of host cells.
6^Study the inhibition of compounds of Echinacea purpure on four kinds of bacteria. The results showed there were no inhibition on bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella and hemolytic streptococcus.
7、Chemical method have been used to study antioxidant activities of cinnamic acid derivatives of Echinacea purpure, the results showed that the antioxidant of compounds were as follows:Cichoric acid,2-caffeoyltartaric acid,2-caffeoyl-3-feruloyltartaric acid,2,3-diisferuloyltartaric acid,2-isferuloyltartaric acid; Structure-activity relationship showed:active groups of antioxidantactive was the phenolic hydroxyl on benzene of caffeic acide groups, and the bit phenolic hydroxyl was the strongest activity and adjacent was weak, but the protective effect of DNA damage does not exist this relationship, indicating that the protective effect has different action mechanism.
8、Simultaneous analysis7cinnamic acid compounds and5alkylamide compounds in Echinacea purpure by HPLC-PDA with gradient elution on a C18reverse phase column in60minutes without the pretreatment of samples, it was suitable as standard analytical method to detect the active ingredients in Echinacea purpure.
引文
[1]Kemper KJ. Complementary and alternative medicine therapies for cold and flu season: What is the science? Natural Health Products. Medscape Family, Medicine.2009
[2]Barrett B. Medicinal properties of echinacea:A critical review. Phytomedicine. 2003;10:66-86
[3]Tierra M. Echinacea:An effective alternative to antibiotics. J Herb Pharmacother. 2007;7:79-89
[4]Bauer R. Pflanzliche immunstimulanzien in der selbstmedikation. Pharmazie in unserer Zeit.1998;27:144-157
[5]Brevoort P. The us botanical market:An overview. HerbalGram.1996
[6]Cheminat A, Zawatzky R, Becker H, et al. Caffeoyl conjugates from echinacea species: Structures and biological activity. Phytochemistry.1988;27:2787-2794
[7]Bauer R, Remiger P, Wagner H. Echinacea—vergleichende dc-und hplc-analyse der herba-drogen von echinacea purpurea, e. Pallida und e. Angustifolia (3. Mitt.). Deutsche Apotheker Zeitung.1988; 128:174-180
[8]Remiger P. Zur chemie und immunologie neuer alkylamide und anderer inhaltsstoffe aus echinacea purpurea, echinacea angustifolia und echinacea pallida.1988.
[9]Becker H, Hsieh WC. Chicoree-saure und deren derivate aus echinacea-arten. Z. Naturforsch.1985:585-587
[10]Soicke H, Al-Hassan G, Gorler K. Weitere kaffeesaure-derivate aus echinacea purpurea. Planta medica.1988;54:175-176
[11]Stoll A, Renz J, Brack A. Isolierung und konstitution des echinacosids, eines glykosids aus den wurzeln von echinacea angustifolia dc 6. Mitteilung uber antibakterielle stoffe. Helvetica Chimica Acta.1950;33:1877-1893
[12]Bohlmann F, Hoffmann H. Further amides from echinacea purpurea. Phytochemistry. 1983;22:1173-1175
[13]Bauer R, Remiger P, Wagner H. Alkamides from the roots of echinacea purpurea. Phytochemistry.1988;27:2339-2342
[14]Perry NB, Van Klink JW, Burgess EJ, et al. Alkamide levels in echinacea purpurea:A rapid analytical method revealing differences among roots, rhizomes, stems, leaves and flowers. Planta Medica-Natural Products and Medicinal Plant Research.1997;63:58-62
[15]Sloley BD, Urichuk LJ, Tywin C, et al. Comparison of chemical components and antioxidant capacity of different echinacea species. Journal of Pharmacy and Pharmacology.2001;53:849-857
[16]Bohlmann F, Grenz M. Polyacetylenverbindungen, cxii. ber die inhaltsstoffe aus echinacea-arten. Chemische Berichte.1966;99:3197-3200
[17]Bauer R, Remiger P, Jurcic K, et al. Beeinflussung der phagozytose-aktivitat durch echinacea-extrakte. Z Phytother.1989; 10:43-48
[18]Binns SE, Livesey JF, Arnason JT, et al. Phytochemical variation in echinacea from roots and flowerheads of wild and cultivated populations. Journal of agricultural and food chemistry.2002;50:3673-3687
[19]Matovic N, Matthias A, Gertsch J, et al. Stereoselective synthesis, natural occurrence and cb2 receptor binding affinities of alkylamides from herbal medicines such as echinacea sp. Org. Biomol. Chem.2006;5:169-174
[20]Perry NB, van Klink JW, Burgess EJ, et al. Alkamide levels in echinacea purpurea: Effects of processing, drying and storage. Planta Med.2000;66:54-56
[21]Wu L, Bae J, Kraus G, et al. Diacetylenic isobutylamides of echinacea:Synthesis and natural distribution. Phytochemistry.2004;65:2477-2484
[22]N B, C B, I W, et al. Immunmodulierende eigenschaften von wurzelextrakten verschiedener echinacea-arten. Z.Phytother.1995:157-166
[23]H W, A P. ber ein immunstimulerendes workprinzip aus echinacea purpurea moench. Z. Angew. Phytother.1981:166-171
[24]Stimpel M, Proksch A, Wagner H, et al. Macrophage activation and induction of macrophage cytotoxicity by purified polysaccharide fractions from the plant echinacea purpurea. Infect Immun.1984;46:845-849
[25]Proksch A, Wagner H. Structural analysis of a 4-o-methyl-glucuronoarabinoxylan with immuno-stimulating activity from echinacea purpurea. Phytochemistry. 1987;26:1989-1993
[26]Wagner H, Stuppner H, Schafer W, et al. Immunologically active polysaccharides of echinacea purpurea cell cultures. Phytochemistry.1988;27:119-126
[27]Bauer R. Chemistry, analysis and immunological investigations of echinacea phytopharmaceuticals. Immunomodulatory agents from plants.1999:41-88
[28]Becker H. Gegen schlangenbiss und grippe-verwendung und inhaltsstoffe von echinacea angustifolia und echinacea purpurea. Dtsch. Apoth. Ztg.1982:2320-2323
[29]Martin R. Saureamide und andere lipophile inhaltsstoffe aus acmella ciliata (hbk) cass. 1985
[30]Malonga-Makosi JP. Untersuchung der flavonoide von echinacea angustifolia-dc-und echinacea purpurea-moench.1983
[31]Roder E, Wiedenfeld H, Hille T, et al. Pyrrolizidine in echinacea angustifolia dc. And echinacea purpurea moench-isolerung und analytik. Dtsch. Apoth. Ztg.1984:2316-2318
[32]Burger RA, Torres AR, Warren RP, et al. Echinacea-induced cytokine production by human macrophages. International journal of immunopharmacology.1997; 19:371-379
[33]Roesler J, Emmendorffer A, Steinmuller C, et al. Application of purified polysaccharides from cell cultures of the plant echinacea purpurea to test subjects mediates activation of the phagocyte system. Int J Immunopharmacol.1991; 13:931-941
[34]Cundell DR, Matrone MA, Ratajczak P, et al. The effect of aerial parts of echinacea on the circulating white cell levels and selected immune functions of the aging male sprague-dawley rat. Int Immunopharmacol.2003;3:1041-1048
[35]Bauer R. Echinacea-drogen-neue ergebnisse zur frage der wirksubstanzen. Natur und Ganzheitsmedizin.1993;6:13-22
[36]Bauer R. Echinacea-eine arzneidroge auf dem weg zum rationalen phytotherapeutikum. Deutsche Apotheker Zeitung.1994; 134:18-28
[37]Classen B, Thude S, Blaschek W, et al. Immunomodulatory effects of arabinogalactan-proteins from baptisia and echinacea. Phytomedicine.2006; 13:688-694
[38]Goel V, Chang C, Slama JV, et al. Alkylamides of echinacea purpurea stimulate alveolar macrophage function in normal rats. Int Immunopharmacol.2002;2:381-387
[39]See DM, Broumand N, Sahl L, et al. In vitro effects of echinacea and ginseng on natural killer and antibody-dependent cell cytotoxicity in healthy subjects and chronic fatigue syndrome or acquired immunodeficiency syndrome patients. Immunopharmacology. 1997;35:229-235
[40]Currier NL, Miller SC. Natural killer cells from aging mice treated with extracts from echinacea purpurea are quantitatively and functionally rejuvenated. Exp Gerontol. 2000;35:627-639
[41]Gan XH, Zhang L, Heber D, et al. Mechanism of activation of human peripheral blood nk cells at the single cell level by echinacea water soluble extracts:Recruitment of lymphocyte-target conjugates and killer cells and activation of programming for lysis. International immunopharmacology.2003;3:811-824
[42]Muller-Jakic B, Breu W, Probstle A, et al. In vitro inhibition of cyclooxygenase and 5-lipoxygenase by alkamides from echinacea and achilles species. Planta Med.1994:37-40
[43]Wagner H, Breu W, Willer F, et al. In vitro inhibition of arachidonate metabolism by some alkamides and prenylated phenols. Planta medica.1989;55:566-567
[44]Randolph R, Gellenbeck K, Stonebrook K, et al. Regulation of human immune gene expression as influenced by a commercial blended echinacea product:Preliminary studies. Experimental Biology and Medicine.2003;228:1051-1056
[45]Brovelli EA, Rua D, Roh-Schmidt H, et al. Human gene expression as a tool to determine horticultural maturity in a bioactive plant (echinacea purpurea 1. Moench). Journal of agricultural and food chemistry.2005;53:8156-8161
[46]Wang CY, Staniforth V, Chiao MT, et al. Genomics and proteomics of immune modulatory effects of a butanol fraction of echinacea purpurea in human dendritic cells. BMC Genomics.2008;9:479
[47]Benson JM, Pokorny AJ, Rhule A, et al. Echinacea purpurea extracts modulate murine dendritic cell fate and function. Food Chem Toxicol.2010;48:1170-1177
[48]Sasagawa M, Cech NB, Gray DE, et al. Echinacea alkylamides inhibit interleukin-2 production by jurkat t cells. International immunopharmacology.2006;6:1214-1221
[49]Gertsch J, Schoop R, Kuenzle U, et al. Echinacea alkylamides modulate tnf-a gene expression via cannabinoid receptor cb2 and multiple signal transduction pathways. FEBS letters.2004;577:563-569
[50]Cech NB, Tutor K, Doty BA, et al. Liver enzyme-mediated oxidation of echinacea purpurea alkylamides:Production of novel metabolites and changes in immunomodulatory activity. Planta Med.2006;72:1372-1377
[51]Spelman K, Iiams-Hauser K, Cech NB, et al. Role for ppargamma in il-2 inhibition in t cells by echinacea-derived undeca-2e-ene-8,10-diynoic acid isobutylamide. Int Immunopharmacol.2009;9:1260-1264
[52]Tunnerhoff F, Schwabe H. Untersuchungen am menschen und am tier uber den einfluss von echinaceakonzentraten auf die kunstliche bindegewebsbildung nach fibrin-implantationen. Heft.2001;6:330-334
[53]Bauer R. Echinacea-drogen:Wirkungen und wirksubstanzen. Zeitschrift fur arztliche Fortbildung.1996;90:111-115
[54]Voitenko GM, Varchenko VG, Lipkan GM, et al. Influence of preparations from the roots and flowers of echinacea purpurea on the course of the inflammatory reaction under experimental conditions. (Ukranian).Farm Zh 1996:115-121
[55]Tragni E, Galli CL, Tubaro A, et al. Anti-inflammatory activity of echinacea angustifolia fractions separated on the basis of molecular weight. Pharmacol Res Commun.1988;20 Suppl 5:87-90
[56]Tragni E, Tubaro A, Melis S, et al. Evidence from two classic irritation tests for an anti-inflammatory action of a natural extract, echinacina b. Food Chem Toxicol. 1985;23:317-319
[57]Tubaro A, Tragni E, Negro P, et al. Anti-inflammatory activity of a polysaccharidic fraction of echinacea angustifolia. Journal of Pharmacy and Pharmacology. 1987;39:567-569
[58]Muller-Jakic B, Breu W, Probstle A, et al. In vitro inhibition of cyclooxygenase and 5-lipoxygenase by alkamides from echinacea and achillea species. Planta Medica-Journal of Medicinal Plant Research.1994;60:37-40
[59]Clifford LJ, Nair MG, Rana J, et al. Bioactivity of alkamides isolated from echinacea purpurea (l.) moench. Phytomedicine.2002;9:249-253
[60]Rininger JA, Kickner S, Chigurupati P, et al. Immunopharmacological activity of echinacea preparations following simulated digestion on murine macrophages and human peripheral blood mononuclear cells. JLeukoc Biol.2000;68:503-510
[61]Hinz B, Woelkart K, Bauer R. Alkamides from echinacea inhibit cyclooxygenase-2 activity in human neuroglioma cells. Biochem Biophys Res Commun.2007;360:441-446
[62]McKay LI, Cidlowski JA. Molecular control of immune/inflammatory responses: Interactions between nuclear factor-kappa b and steroid receptor-signaling pathways. Endocrine Reviews 1999:435-459
[63]Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the nf-kappab pathway in the treatment of inflammation and cancer. J Clin Invest.2001;107:135-142
[64]Meurs H, Maarsingh H, Zaagsma J. Arginase and asthma:Novel insights into nitric oxide homeostasis and airway hyperresponsiveness. Trends in pharmacological sciences. 2003;24:450-455
[65]Man-Ying Chan M, Mattiacci JA, Hwang HS, et al. Synergy between ethanol and grape polyphenols, quercetin, and resveratrol, in the inhibition of the inducible nitric oxide synthase pathway. Biochemical pharmacology.2000;60:1539-1548
[66]Zhai Z, Solco A, Wu L, et al. Echinacea increases arginase activity and has anti-inflammatory properties in raw 264.7 macrophage cells, indicative of alternative macrophage activation. Journal of ethnopharmacology.2009; 122:76-85
[67]Chen XM, Hu C, Raghubeer E, et al. Effect of high pressure pasteurization on bacterial load and bioactiviry of echinacea purpurea. JFood Sci.2010;75:C613-618
[68]Guiotto P, Woelkart K, Grabnar I, et al. Pharmacokinetics and immunomodulatory effects of phytotherapeutic lozenges (bonbons) with echinacea purpurea extract. Phytomedicine. 2008;15:547-554
[69]Woelkart K, Bauer R. The role of alkamides as an active principle of echinacea. Planta Medica-Natural Products and Medicinal Plant Research.2007;73:615-623
[70]Matthias A, Banbury L, Bone KM, et al. Echinacea alkylamides modulate induced immune responses in t-cells. Fitoterapia.2008;79:53-58
[71]Hudson J, Vimalanathan S, Kang L, et al. Characterization of antiviral activities in echinacea. Root preparations. Pharmaceutical biology.2005;43:790-796
[72]Vimalanathan S, Kang L, Amiguet VT, et al. Echinacea purpurea. Aerial parts contain multiple antiviral compounds. Pharmaceutical biology.2005;43:740-745
[73]Pleschka S, Stein M, Schoop R, et al. Anti-viral properties and mode of action of standardized echinacea purpurea extract against highly pathogenic avian influenza virus (h5nl, h7n7) and swine-origin h1n1 (s-oiv). Virol J.2009;6:197
[74]Sharma M, Anderson SA, Schoop R, et al. Induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized echinacea, a potent antiviral herbal extract. Antiviral Res.2009;83:165-170
[75]Vohra S, Adams D, Hudson JB, et al. Selection of natural health products for clinical trials:A preclinical template. Can JPhysiol Pharmacol.2009;87:371-378
[76]Sharma SM, Anderson M, Schoop SR, et al. Bactericidal and anti-inflammatory properties of a standardized echinacea extract (echinaforce):Dual actions against respiratory bacteria. Phytomedicine.2010; 17:563-568
[77]Fusco D, Liu X, Savage C, et al. Echinacea purpurea aerial extract alters course of influenza infection in mice. Vaccine.2010;28:3956-3962
[78]Sharma M, Schoop R, Hudson J. Echinacea as an antiinflammatory agent:The influence of physiologically relevant parameters. Phytotherapy Research.2009;23:863-867
[79]Hudson JB. The multiple actions of the phytomedicine echinacea in the treatment of colds and flu. Journal of Medicinal Plant Research.2010;4:2746-2752
[80]Sharma M, Arnason J, Hudson J. Echinacea extracts modulate the production of multiple transcription factors in uninfected cells and rhinovirus-infected cells. Phytotherapy Research.2006;20:1074-1079
[81]Sharma M, Arnason JT, Hudson JB. Echinacea extracts modulate the production of multiple transcription factors in uninfected cells and rhinovirus-infected cells. Phytother Res.2006;20:1074-1079
[82]Fuchs R, Blaas D. Uncoating of human rhinoviruses. Reviews in medical virology. 2010;20:281-297
[83]Pleschka S, Stein M, Schoop R, et al. Anti-viral properties and mode of action of standardized echinacea purpurea extract against highly pathogenic avian influenza virus (h5n1,h7n7) and swine-origin hlnl (s-oiv). Virol J.2009;6:197
[84]Sharma M, Anderson SA, Schoop R, et al. Induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized< i> echinacea, a potent antiviral herbal extract. Antiviral research.2009;83:165-170
[85]Reinke RA, Lee DJ, McDougall BR, et al. L-chicoric acid inhibits human immunodeficiency virus type 1 integration in vivo and is a noncompetitive but reversible inhibitor of hiv-1 integrase in vitro. Virology.2004;326:203-219
[86]Edward Robinson W. L-chicoric acid, an inhibitor of human immunodeficiency virus type 1 (hiv-1) integrase, improves on the in vitro anti-hiv-1 effect of zidovudine plus a protease inhibitor (ag 1350). Antiviral research.1998;39:101-111
[87]Richardson MA, Sanders T, Palmer JL, et al. Complementary/alternative medicine use in a comprehensive cancer center and the implications for oncology. Journal of Clinical Oncology.2000; 18:2505-2514
[88]Lersch C, Zeuner M, Bauer A, et al. Nonspecific immunostimulation with low doses of cyclophosphamide (ldcy), thymostimulin, and echinacea purpurea extracts (echinacin) in patients with far advanced colorectal cancers:Preliminary results. Cancer investigation. 1992;10:343-348
[89]Chicca A, Adinolfi B, Martinotti E, et al. Cytotoxic effects of< i> echinacea root hexanic extracts on human cancer cell lines. Journal of ethnopharmacology. 2007; 110:148-153
[90]Pellati F, Cain S, Benvenuti S, et al. Isolation and structure elucidation of cytotoxic polyacetylenes and polyenes from echinacea pallida. Phytochemistry.2006;67:1359-1364
[91]Hu C, Kitts DD. Studies on the antioxidant activity of echinacea root extract. J Agric Food Chem.2000;48:1466-1472
[92]Merali S, Binns S, Paulin-Levasseur M, et al. Antifungal and anti-inflammatory activity of the genus echinacea. Pharmaceutical biology.2003;41:412-420
[93]Facino RM, Carini M, Aldini G, et al. Echinacoside and caffeoyl conjugates protect collagen from free radical-induced degradation:A potential use of echinacea extracts in the prevention of skin photodamage. Planta Med.1995;61:510-514
[94]Matthias A, Blanchfield J, Penman K, et al. Permeability studies of alkylamides and caffeic acid conjugates from echinacea using a caco-2 cell monolayer model. Journal of clinical pharmacy and therapeutics.2004;29:7-13
[95]Matthias A, Addison R, Agnew L, et al. Comparison of echinacea alkylamide pharmacokinetics between liquid and tablet preparations. Phytomedicine. 2007;14:587-590
[96]Matthias A, Addison R, Penman K, et al. Echinacea alkamide disposition and pharmacokinetics in humans after tablet ingestion. Life sciences.2005;77:2018-2029
[97]Woelkart K, Marth E, Suter A, et al. Bioavailability and pharmacokinetics of echinacea purpurea preparations and their interaction with the immune system. International journal of clinical pharmacology and therapeutics.2006;44:401-408
[98]陈波,潘振球.高效液相色谱法制备菊苣酸标准对照品的研究.实用预防医学.2001；8：15-17
[99]Wang X, Geng Y, Li F, et al. Preparative separation of cichoric acid from echinacea purpurea by ph-zone-refining counter-current chromatography. J Chromatogr A. 2006; 1103:166-169
[100]陈养文.紫锥菊中菊芭酸提取纯化工艺研究.中草药.2004；9995-997
[101]刘轶琛,曾建国,陈波,et al.聚酰胺对紫锥菊提取物中菊苣酸的分离纯化研究.中草药.2007；38：530-533
[102]Perry NB, Burgess EJ, leAnne Glennie V. Echinacea standardization:Analytical methods for phenolic compounds and typical levels in medicinal species. Journal of agricultural and food chemistry.2001;49:1702-1706
[103]Pellati F, Benvenuti S, Melegari M, et al. Variability in the composition of anti-oxidant compounds in echinacea species by hplc. Phytochemical Analysis.2005; 16:77-85
[104]徐度,唐宇伟,朱伟Rp-hplc测定紫锥菊提取物中2种咖啡酸衍生物的含量.中成药.2006；28：1493-1497
[105]Gocan S, Radu L, Hadaruga M. Simultaneous analysis by high performance liquid chromatography of hydrophilic compounds and lipophilic compounds (alkamides) from pharmaceutical preparations ofechinacea purpurea root. Chromatographia. 2003;57:677-679
[106]Molgaard P, Johnsen S, Christensen P, et al. Hplc method validated for the simultaneous analysis of cichoric acid and alkamides in echinacea purpurea plants and products. J Agric Food Chem.2003;51:6922-6933
[107]Cech NB, Eleazer MS, Shoffner LT, et al. High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from< i> echinacea purpurea extracts. Journal of chromatography A.2006; 1103:219-228
[108]Luo XB, Chen B, Yao SZ, et al. Simultaneous analysis of caffeic acid derivatives and alkamides in roots and extracts of< i> echinacea purpurea by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry. Journal of chromatography A.2003;986:73-81
[109]Gotti R, Pomponio R, Bertucci C, et al. Simultaneous analysis of the lipophilic and hydrophilic markers of echinacea plant extracts by capillary electrophoresis. Journal of separation science.2002;25:1079-1086
[110]Mancek B, Kreft S. Determination of cichoric acid content in dried press juice of purple coneflower (echinacea purpurea) with capillary electrophoresis. Talanta. 2005;66:1094-1097
[111]Pomponio R, Gotti R, Hudaib M, et al. Analysis of phenolic acids by micellar electrokinetic chromatography:Application to< i> echinacea purpurea plant extracts. Journal ofchromatographyA.2002;945:239-247
[112]黄劲梅,谢培山.紫锥花及其制剂中菊苣酸的薄层扫描含量测定.中药新药与临床药理,2002,13(1)：33-3.2002；13：33-35
[113]Tsukui A, Kuwano K, Mitamura T. Anthocyanin pigment isolated from purple root of sweetpotato. Nihon Kaseigaku Zasshi.1983;34:153-159
[114]Odake K, Terahara N, Saito N, et al. Chemical structures of two anthocyanins from purple sweet potato, ipomoea batatas. Phytochemistry.1992;31:2127-2130
[115]Goda Y, Shimizu T, Kato Y, et al. Two acylated anthocyanins from purple sweet potato. Phytochemistry.1997;44:183-186
[116]Terahara N, Shimizu T, Kato Y, et al. Six diacylated anthocyanins from the storage roots of purple sweet potato, ipomoea batatas. Bioscience, biotechnology, and biochemistry. 1999;63:1420-1424
[117]姜平平.花色苷类物质抗氧化生理活性的研究[d].2003
[118]Matsui T, Ebuchi S, Kobayashi M, et al. Anti-hyperglycemic effect of diacylated anthocyanin derived from ipomoea batatas cultivar ayamurasaki can be achieved through the a-glucosidase inhibitory action. Journal of agricultural and food chemistry. 2002;50:7244-7248
[119]Yoshimoto M, Okuno S, Yoshinaga M, et al. Antimutagenicity of sweetpotato (ipomoea batatas) roots. Bioscience, biotechnology, and biochemistry.1999;63:537-541
[120]方忠祥,倪元颖.花青素生理功能研究进展.广州食品工业科技.2001；17：60-62
[121]岳静,杰宏筠.紫甘薯红色素体外抑菌性初探.辽宁农业科学.2005：47-47
[122]Suda I, Yamakawa O, Matsugano K, et al. Changes of serum gamma-gtp, got and gpt levels in hepatic function-weakling subjects by ingestion of high anthocyanin sweetpotato juice. Journal of the Japanese Society for Food Science and Technology.1998;45
[123]Suda I, Furuta S, Nishiba Y, et al. Reduction of liver injury induced by carbon tetrachloride in rats administered purple colored sweetpotato juice. Journal of the Japanese Society for Food Science and Technology.1997;44:315-318
[124]Yamakawa O, Suda I, Yoshimoto M. Development and utilization of sweetpotato cultivars with high anthocyanin content. FOODS AND FOOD INGREDIENTS JOURNAL OF JAPAN.1998:69-78
[125]Wang K, Liu Z, Huang JA, et al. Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2008;867:282-286
[126]Gao M, Gu M, Liu CZ. Two-step purification of scutellarin from erigeron breviscapus (vant.) hand. Mazz. By high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2006;838:139-143
[127]Cao X, Tian Y, Zhang T, et al. Separation and purification of isoflavones from pueraria lobata by high-speed counter-current chromatography. J Chromatogr A. 1999;855:709-713
[128]Du Q, Li Z, Ito Y. Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography. Journal of chromatography A. 2001;923:271-274
[129]Peng J, Zhan L, Dong F, et al. A comparative study of chromatographic methods for separating chemical compounds from< i> spiranthes australis(r. Brown) lindl roots. Separation and Purification Technology.2008;59:262-269
[130]孙印石,刘政波,王建华,et a1.高速逆流色谱分离制备陈皮中的黄酮类化合物.色谱.2009；2009
[131]赵淑杰,韩梅,韩忠明,et al.高速逆流色谱分离与鉴定鹿药中黄酮类化合物.分析化学.2009；37：1354-1358
[132]徐渊金.杨梅和桑葚花色苷的提取分离、结构鉴定及其生物活性研究.浙江工商大学.2007
[133]Degenhardt A, Engelhardt UH, Lakenbrink C, et al. Preparative separation of polyphenols from tea by high-speed countercurrent chromatography. JAgric Food Chem. 2000;48:3425-3430
[134]Schwarz M, Wray V, Winterhalter P. Isolation and identification of novel pyranoanthocyanins from black carrot (daucus carota 1.) juice. J Agric Food Chem. 2004;52:5095-5101
[135]胡晓丹.紫苏中次生代谢物的现代提取分离技术及性质研究[d].2008
[136]李佳银,罗晋,李觅路,et al.高速逆流色谱法分离紫锥菊花色苷及其抗氧化性研究.分析测试学报.2012；1
[137]Tang Q, Yang C, Ye W, et al. Preparative isolation and purification of bioactive constituents from< i> aconitum coreanum by high-speed counter-current chromatography coupled with evaporative light scattering detection. Journal of chromatography A.2007;1144:203-207
[138]Liu R, Chu X, Sun A, et al. Preparative isolation and purification of alkaloids from the chinese medicinal herb< i> evodia rutaecarpa(juss.) benth by high-speed counter-current chromatography. Journal of chromatography A.2005; 1074:139-144
[139]Liu Z, Jin Y, Shen P, et al. Separation and purification of verticine and verticinone from< i> bulbus fritillariae thunbergii by high-speed counter-current chromatography coupled with evaporative light scattering detection. Talanta.2007;71:1873-1876
[140]吴三桥,冯自立,李新生,et al.高速逆流色谱法分离纯化延胡索乙素和原阿片碱.中国生化药物杂志.2009；30：89-92
[141]褚建军,颜继忠.制备型高速逆流色谱分离中药中的生物碱.浙江工业大学学报.2006；34
[142]Yang C, Li D, Wan X. Combination of hscce and sephadex lh-20 methods an approach to isolation and purification of the main individual theaflavins from black tea. J Chromatogr B Analyt Technol Biomed Life Sci.2008;861:140-144
[143]Geng Y, Liu J, Lv R, et al. An efficient method for extraction, separation and purification of eugenol from eugenia caryophyllata by supercritical fluid extraction and high-speed counter-current chromatography. Separation and Purification Technology. 2007;57:237-241
[144]Shi S, Huang K, Zhang Y, et al. Purification and identification of antiviral components from laggera pterodonta by high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2007;859:119-124
[145]
[146]Becker H, Hsieh WC. Chicoree-saure und deren derivate aus echinacea-arten. Zeitschrift fur Naturforschung. Section C, Biosciences.1985;40:585-587
[147]Yasuda I, Takeya K, Itokawa H. Structures of amides from asiasarum heterotropoides maek. Var. Mandshuricum maek. Chem. Pharm. Bull.1981;29:564-566
[148]Suda I, Oki T, Masuda M, et al. Direct absorption of acylated anthocyanin in purple-fleshed sweet potato into rats. Journal of agricultural and food chemistry. 2002;50:1672-1676
[149]Kano M, Takayanagi T, Harada K, et al. Antioxidative activity of anthocyanins from purple sweet potato, ipomoera batatas cultivar ayamurasaki. Bioscience, biotechnology, and biochemistry.2005;69:979-988
[150]Suda I, Furuta S, Nishiba Y, et al. Hepato-protective activity of purple-colored sweetpotato juice. Sweetpotato Research Front.1997;4
[151]韩永斌,朱洪梅,顾振新,et al.紫甘薯花色苷色素的抑菌作用研究.微生物学通报.2008；35：913-917
[152]Harborne J. Spectral methods of characterizing anthocyanins. Biochemical Journal. 1958;70:22
[153]滕荣伟,周志宏,王德祖,et al.白花刺参中的咖啡酰基奎宁酸成分.波学学杂志.2002；19：167-174
[154]Islam MS, Yoshimoto M, Yahara S, et al. Identification and characterization of foliar polyphenolic composition in sweetpotato (ipomoea batatas 1.) genotypes. Journal of agricultural and food chemistry.2002;50:3718-3722
[155]Von Itzstein M. The war against influenza:Discovery and development of sialidase inhibitors. Nature Reviews Drug Discovery.2007;6:967-974
[156]Ohuchi M, Asaoka N, Sakai T, et al. Roles of neuraminidase in the initial stage of influenza virus infection. Microbes and infection.2006;8:1287-1293
[157]Moscona A. Medical management of influenza infection. Annu. Rev. Med. 2008;59:397-413
[158]Garman E, Laver G.Controlling influenza by inhibiting the viruss neuraminidase. Current drug targets.2004;5:119-136
[159]Nayak DP, Reich1 U. Neuraminidase activity assays for monitoring mdck cell culture derived influenza virus. Journal of virological methods.2004;122:9-15
[160]Ryu YB, Curtis-Long MJ, Kim JH, et al. Pterocarpans and flavanones from sophora flavescens displaying potent neuraminidase inhibition. Bioorg Med Chem Lett. 2008; 18:6046-6049
[161]高雷,章天,陈鸿珊.黄芩甙对流感病毒神经氨酸酶的抑制作用.中国现代医生.2007；45：46-46
[162]Cuzzocrea S, Riley DP, Caputi AP, et al. Antioxidant therapy:A new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacological reviews.2001;53:135-159
[163]Jun PZ, Yuan C, Mei Z. The effect of polysaccharide krestin on gpx gene expression in macrophages [j]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA.1999;3
[164]赵艳红,李建科,李国秀.天然抗氧化物体外活性评价方法的优选与优化.食品科学.2008；29：64-69
[165]Cotelle N, Bernier JL, Catteau JP, et al. Antioxidant properties of hydroxy-flavones. Free Radical Biology and Medicine.1996;20:35-43
[166]Amarowicz R, Pegg R, Rahimi-Moghaddam P, et al. Free-radical scavenging capacity and antioxidant activity of selected plant species from the canadian prairies. Food Chemistry.2004;84:551-562
[167]田卉,马建慧,张梅,et al.石榴皮的体外抗脂质过氧化作用.中药药理与临床.2009；25：69-70
[168]董欢欢,曹树稳,余燕影.半枝莲,白花蛇舌草及其药对提取物抗氧化及清除自由基活性.无然产物研究与开发.2008；20：782-786
[169]Ningappa MB, Dinesha R, Srinivas L. Antioxidant and free radical scavenging activities of polyphenol-enriched curry leaf (murraya koenigii 1.) extracts. Food Chemistry. 2008; 106:720-728
[170]孙庆雷,王晓,刘建华,et al.黄酮类化合物抗氧化反应性的构效关系.食品科学.2005；26：69-72
[2]Barrett B. Medicinal properties of echinacea:A critical review. Phytomedicine. 2003;10:66-86
[3]Tierra M. Echinacea:An effective alternative to antibiotics. J Herb Pharmacother. 2007;7:79-89
[4]Bauer R. Pflanzliche immunstimulanzien in der selbstmedikation. Pharmazie in unserer Zeit.1998;27:144-157
[5]Brevoort P. The us botanical market:An overview. HerbalGram.1996
[6]Cheminat A, Zawatzky R, Becker H, et al. Caffeoyl conjugates from echinacea species: Structures and biological activity. Phytochemistry.1988;27:2787-2794
[7]Bauer R, Remiger P, Wagner H. Echinacea—vergleichende dc-und hplc-analyse der herba-drogen von echinacea purpurea, e. Pallida und e. Angustifolia (3. Mitt.). Deutsche Apotheker Zeitung.1988; 128:174-180
[8]Remiger P. Zur chemie und immunologie neuer alkylamide und anderer inhaltsstoffe aus echinacea purpurea, echinacea angustifolia und echinacea pallida.1988.
[9]Becker H, Hsieh WC. Chicoree-saure und deren derivate aus echinacea-arten. Z. Naturforsch.1985:585-587
[10]Soicke H, Al-Hassan G, Gorler K. Weitere kaffeesaure-derivate aus echinacea purpurea. Planta medica.1988;54:175-176
[11]Stoll A, Renz J, Brack A. Isolierung und konstitution des echinacosids, eines glykosids aus den wurzeln von echinacea angustifolia dc 6. Mitteilung uber antibakterielle stoffe. Helvetica Chimica Acta.1950;33:1877-1893
[12]Bohlmann F, Hoffmann H. Further amides from echinacea purpurea. Phytochemistry. 1983;22:1173-1175
[13]Bauer R, Remiger P, Wagner H. Alkamides from the roots of echinacea purpurea. Phytochemistry.1988;27:2339-2342
[14]Perry NB, Van Klink JW, Burgess EJ, et al. Alkamide levels in echinacea purpurea:A rapid analytical method revealing differences among roots, rhizomes, stems, leaves and flowers. Planta Medica-Natural Products and Medicinal Plant Research.1997;63:58-62
[15]Sloley BD, Urichuk LJ, Tywin C, et al. Comparison of chemical components and antioxidant capacity of different echinacea species. Journal of Pharmacy and Pharmacology.2001;53:849-857
[16]Bohlmann F, Grenz M. Polyacetylenverbindungen, cxii. ber die inhaltsstoffe aus echinacea-arten. Chemische Berichte.1966;99:3197-3200
[17]Bauer R, Remiger P, Jurcic K, et al. Beeinflussung der phagozytose-aktivitat durch echinacea-extrakte. Z Phytother.1989; 10:43-48
[18]Binns SE, Livesey JF, Arnason JT, et al. Phytochemical variation in echinacea from roots and flowerheads of wild and cultivated populations. Journal of agricultural and food chemistry.2002;50:3673-3687
[19]Matovic N, Matthias A, Gertsch J, et al. Stereoselective synthesis, natural occurrence and cb2 receptor binding affinities of alkylamides from herbal medicines such as echinacea sp. Org. Biomol. Chem.2006;5:169-174
[20]Perry NB, van Klink JW, Burgess EJ, et al. Alkamide levels in echinacea purpurea: Effects of processing, drying and storage. Planta Med.2000;66:54-56
[21]Wu L, Bae J, Kraus G, et al. Diacetylenic isobutylamides of echinacea:Synthesis and natural distribution. Phytochemistry.2004;65:2477-2484
[22]N B, C B, I W, et al. Immunmodulierende eigenschaften von wurzelextrakten verschiedener echinacea-arten. Z.Phytother.1995:157-166
[23]H W, A P. ber ein immunstimulerendes workprinzip aus echinacea purpurea moench. Z. Angew. Phytother.1981:166-171
[24]Stimpel M, Proksch A, Wagner H, et al. Macrophage activation and induction of macrophage cytotoxicity by purified polysaccharide fractions from the plant echinacea purpurea. Infect Immun.1984;46:845-849
[25]Proksch A, Wagner H. Structural analysis of a 4-o-methyl-glucuronoarabinoxylan with immuno-stimulating activity from echinacea purpurea. Phytochemistry. 1987;26:1989-1993
[26]Wagner H, Stuppner H, Schafer W, et al. Immunologically active polysaccharides of echinacea purpurea cell cultures. Phytochemistry.1988;27:119-126
[27]Bauer R. Chemistry, analysis and immunological investigations of echinacea phytopharmaceuticals. Immunomodulatory agents from plants.1999:41-88
[28]Becker H. Gegen schlangenbiss und grippe-verwendung und inhaltsstoffe von echinacea angustifolia und echinacea purpurea. Dtsch. Apoth. Ztg.1982:2320-2323
[29]Martin R. Saureamide und andere lipophile inhaltsstoffe aus acmella ciliata (hbk) cass. 1985
[30]Malonga-Makosi JP. Untersuchung der flavonoide von echinacea angustifolia-dc-und echinacea purpurea-moench.1983
[31]Roder E, Wiedenfeld H, Hille T, et al. Pyrrolizidine in echinacea angustifolia dc. And echinacea purpurea moench-isolerung und analytik. Dtsch. Apoth. Ztg.1984:2316-2318
[32]Burger RA, Torres AR, Warren RP, et al. Echinacea-induced cytokine production by human macrophages. International journal of immunopharmacology.1997; 19:371-379
[33]Roesler J, Emmendorffer A, Steinmuller C, et al. Application of purified polysaccharides from cell cultures of the plant echinacea purpurea to test subjects mediates activation of the phagocyte system. Int J Immunopharmacol.1991; 13:931-941
[34]Cundell DR, Matrone MA, Ratajczak P, et al. The effect of aerial parts of echinacea on the circulating white cell levels and selected immune functions of the aging male sprague-dawley rat. Int Immunopharmacol.2003;3:1041-1048
[35]Bauer R. Echinacea-drogen-neue ergebnisse zur frage der wirksubstanzen. Natur und Ganzheitsmedizin.1993;6:13-22
[36]Bauer R. Echinacea-eine arzneidroge auf dem weg zum rationalen phytotherapeutikum. Deutsche Apotheker Zeitung.1994; 134:18-28
[37]Classen B, Thude S, Blaschek W, et al. Immunomodulatory effects of arabinogalactan-proteins from baptisia and echinacea. Phytomedicine.2006; 13:688-694
[38]Goel V, Chang C, Slama JV, et al. Alkylamides of echinacea purpurea stimulate alveolar macrophage function in normal rats. Int Immunopharmacol.2002;2:381-387
[39]See DM, Broumand N, Sahl L, et al. In vitro effects of echinacea and ginseng on natural killer and antibody-dependent cell cytotoxicity in healthy subjects and chronic fatigue syndrome or acquired immunodeficiency syndrome patients. Immunopharmacology. 1997;35:229-235
[40]Currier NL, Miller SC. Natural killer cells from aging mice treated with extracts from echinacea purpurea are quantitatively and functionally rejuvenated. Exp Gerontol. 2000;35:627-639
[41]Gan XH, Zhang L, Heber D, et al. Mechanism of activation of human peripheral blood nk cells at the single cell level by echinacea water soluble extracts:Recruitment of lymphocyte-target conjugates and killer cells and activation of programming for lysis. International immunopharmacology.2003;3:811-824
[42]Muller-Jakic B, Breu W, Probstle A, et al. In vitro inhibition of cyclooxygenase and 5-lipoxygenase by alkamides from echinacea and achilles species. Planta Med.1994:37-40
[43]Wagner H, Breu W, Willer F, et al. In vitro inhibition of arachidonate metabolism by some alkamides and prenylated phenols. Planta medica.1989;55:566-567
[44]Randolph R, Gellenbeck K, Stonebrook K, et al. Regulation of human immune gene expression as influenced by a commercial blended echinacea product:Preliminary studies. Experimental Biology and Medicine.2003;228:1051-1056
[45]Brovelli EA, Rua D, Roh-Schmidt H, et al. Human gene expression as a tool to determine horticultural maturity in a bioactive plant (echinacea purpurea 1. Moench). Journal of agricultural and food chemistry.2005;53:8156-8161
[46]Wang CY, Staniforth V, Chiao MT, et al. Genomics and proteomics of immune modulatory effects of a butanol fraction of echinacea purpurea in human dendritic cells. BMC Genomics.2008;9:479
[47]Benson JM, Pokorny AJ, Rhule A, et al. Echinacea purpurea extracts modulate murine dendritic cell fate and function. Food Chem Toxicol.2010;48:1170-1177
[48]Sasagawa M, Cech NB, Gray DE, et al. Echinacea alkylamides inhibit interleukin-2 production by jurkat t cells. International immunopharmacology.2006;6:1214-1221
[49]Gertsch J, Schoop R, Kuenzle U, et al. Echinacea alkylamides modulate tnf-a gene expression via cannabinoid receptor cb2 and multiple signal transduction pathways. FEBS letters.2004;577:563-569
[50]Cech NB, Tutor K, Doty BA, et al. Liver enzyme-mediated oxidation of echinacea purpurea alkylamides:Production of novel metabolites and changes in immunomodulatory activity. Planta Med.2006;72:1372-1377
[51]Spelman K, Iiams-Hauser K, Cech NB, et al. Role for ppargamma in il-2 inhibition in t cells by echinacea-derived undeca-2e-ene-8,10-diynoic acid isobutylamide. Int Immunopharmacol.2009;9:1260-1264
[52]Tunnerhoff F, Schwabe H. Untersuchungen am menschen und am tier uber den einfluss von echinaceakonzentraten auf die kunstliche bindegewebsbildung nach fibrin-implantationen. Heft.2001;6:330-334
[53]Bauer R. Echinacea-drogen:Wirkungen und wirksubstanzen. Zeitschrift fur arztliche Fortbildung.1996;90:111-115
[54]Voitenko GM, Varchenko VG, Lipkan GM, et al. Influence of preparations from the roots and flowers of echinacea purpurea on the course of the inflammatory reaction under experimental conditions. (Ukranian).Farm Zh 1996:115-121
[55]Tragni E, Galli CL, Tubaro A, et al. Anti-inflammatory activity of echinacea angustifolia fractions separated on the basis of molecular weight. Pharmacol Res Commun.1988;20 Suppl 5:87-90
[56]Tragni E, Tubaro A, Melis S, et al. Evidence from two classic irritation tests for an anti-inflammatory action of a natural extract, echinacina b. Food Chem Toxicol. 1985;23:317-319
[57]Tubaro A, Tragni E, Negro P, et al. Anti-inflammatory activity of a polysaccharidic fraction of echinacea angustifolia. Journal of Pharmacy and Pharmacology. 1987;39:567-569
[58]Muller-Jakic B, Breu W, Probstle A, et al. In vitro inhibition of cyclooxygenase and 5-lipoxygenase by alkamides from echinacea and achillea species. Planta Medica-Journal of Medicinal Plant Research.1994;60:37-40
[59]Clifford LJ, Nair MG, Rana J, et al. Bioactivity of alkamides isolated from echinacea purpurea (l.) moench. Phytomedicine.2002;9:249-253
[60]Rininger JA, Kickner S, Chigurupati P, et al. Immunopharmacological activity of echinacea preparations following simulated digestion on murine macrophages and human peripheral blood mononuclear cells. JLeukoc Biol.2000;68:503-510
[61]Hinz B, Woelkart K, Bauer R. Alkamides from echinacea inhibit cyclooxygenase-2 activity in human neuroglioma cells. Biochem Biophys Res Commun.2007;360:441-446
[62]McKay LI, Cidlowski JA. Molecular control of immune/inflammatory responses: Interactions between nuclear factor-kappa b and steroid receptor-signaling pathways. Endocrine Reviews 1999:435-459
[63]Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the nf-kappab pathway in the treatment of inflammation and cancer. J Clin Invest.2001;107:135-142
[64]Meurs H, Maarsingh H, Zaagsma J. Arginase and asthma:Novel insights into nitric oxide homeostasis and airway hyperresponsiveness. Trends in pharmacological sciences. 2003;24:450-455
[65]Man-Ying Chan M, Mattiacci JA, Hwang HS, et al. Synergy between ethanol and grape polyphenols, quercetin, and resveratrol, in the inhibition of the inducible nitric oxide synthase pathway. Biochemical pharmacology.2000;60:1539-1548
[66]Zhai Z, Solco A, Wu L, et al. Echinacea increases arginase activity and has anti-inflammatory properties in raw 264.7 macrophage cells, indicative of alternative macrophage activation. Journal of ethnopharmacology.2009; 122:76-85
[67]Chen XM, Hu C, Raghubeer E, et al. Effect of high pressure pasteurization on bacterial load and bioactiviry of echinacea purpurea. JFood Sci.2010;75:C613-618
[68]Guiotto P, Woelkart K, Grabnar I, et al. Pharmacokinetics and immunomodulatory effects of phytotherapeutic lozenges (bonbons) with echinacea purpurea extract. Phytomedicine. 2008;15:547-554
[69]Woelkart K, Bauer R. The role of alkamides as an active principle of echinacea. Planta Medica-Natural Products and Medicinal Plant Research.2007;73:615-623
[70]Matthias A, Banbury L, Bone KM, et al. Echinacea alkylamides modulate induced immune responses in t-cells. Fitoterapia.2008;79:53-58
[71]Hudson J, Vimalanathan S, Kang L, et al. Characterization of antiviral activities in echinacea. Root preparations. Pharmaceutical biology.2005;43:790-796
[72]Vimalanathan S, Kang L, Amiguet VT, et al. Echinacea purpurea. Aerial parts contain multiple antiviral compounds. Pharmaceutical biology.2005;43:740-745
[73]Pleschka S, Stein M, Schoop R, et al. Anti-viral properties and mode of action of standardized echinacea purpurea extract against highly pathogenic avian influenza virus (h5nl, h7n7) and swine-origin h1n1 (s-oiv). Virol J.2009;6:197
[74]Sharma M, Anderson SA, Schoop R, et al. Induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized echinacea, a potent antiviral herbal extract. Antiviral Res.2009;83:165-170
[75]Vohra S, Adams D, Hudson JB, et al. Selection of natural health products for clinical trials:A preclinical template. Can JPhysiol Pharmacol.2009;87:371-378
[76]Sharma SM, Anderson M, Schoop SR, et al. Bactericidal and anti-inflammatory properties of a standardized echinacea extract (echinaforce):Dual actions against respiratory bacteria. Phytomedicine.2010; 17:563-568
[77]Fusco D, Liu X, Savage C, et al. Echinacea purpurea aerial extract alters course of influenza infection in mice. Vaccine.2010;28:3956-3962
[78]Sharma M, Schoop R, Hudson J. Echinacea as an antiinflammatory agent:The influence of physiologically relevant parameters. Phytotherapy Research.2009;23:863-867
[79]Hudson JB. The multiple actions of the phytomedicine echinacea in the treatment of colds and flu. Journal of Medicinal Plant Research.2010;4:2746-2752
[80]Sharma M, Arnason J, Hudson J. Echinacea extracts modulate the production of multiple transcription factors in uninfected cells and rhinovirus-infected cells. Phytotherapy Research.2006;20:1074-1079
[81]Sharma M, Arnason JT, Hudson JB. Echinacea extracts modulate the production of multiple transcription factors in uninfected cells and rhinovirus-infected cells. Phytother Res.2006;20:1074-1079
[82]Fuchs R, Blaas D. Uncoating of human rhinoviruses. Reviews in medical virology. 2010;20:281-297
[83]Pleschka S, Stein M, Schoop R, et al. Anti-viral properties and mode of action of standardized echinacea purpurea extract against highly pathogenic avian influenza virus (h5n1,h7n7) and swine-origin hlnl (s-oiv). Virol J.2009;6:197
[84]Sharma M, Anderson SA, Schoop R, et al. Induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized< i> echinacea, a potent antiviral herbal extract. Antiviral research.2009;83:165-170
[85]Reinke RA, Lee DJ, McDougall BR, et al. L-chicoric acid inhibits human immunodeficiency virus type 1 integration in vivo and is a noncompetitive but reversible inhibitor of hiv-1 integrase in vitro. Virology.2004;326:203-219
[86]Edward Robinson W. L-chicoric acid, an inhibitor of human immunodeficiency virus type 1 (hiv-1) integrase, improves on the in vitro anti-hiv-1 effect of zidovudine plus a protease inhibitor (ag 1350). Antiviral research.1998;39:101-111
[87]Richardson MA, Sanders T, Palmer JL, et al. Complementary/alternative medicine use in a comprehensive cancer center and the implications for oncology. Journal of Clinical Oncology.2000; 18:2505-2514
[88]Lersch C, Zeuner M, Bauer A, et al. Nonspecific immunostimulation with low doses of cyclophosphamide (ldcy), thymostimulin, and echinacea purpurea extracts (echinacin) in patients with far advanced colorectal cancers:Preliminary results. Cancer investigation. 1992;10:343-348
[89]Chicca A, Adinolfi B, Martinotti E, et al. Cytotoxic effects of< i> echinacea root hexanic extracts on human cancer cell lines. Journal of ethnopharmacology. 2007; 110:148-153
[90]Pellati F, Cain S, Benvenuti S, et al. Isolation and structure elucidation of cytotoxic polyacetylenes and polyenes from echinacea pallida. Phytochemistry.2006;67:1359-1364
[91]Hu C, Kitts DD. Studies on the antioxidant activity of echinacea root extract. J Agric Food Chem.2000;48:1466-1472
[92]Merali S, Binns S, Paulin-Levasseur M, et al. Antifungal and anti-inflammatory activity of the genus echinacea. Pharmaceutical biology.2003;41:412-420
[93]Facino RM, Carini M, Aldini G, et al. Echinacoside and caffeoyl conjugates protect collagen from free radical-induced degradation:A potential use of echinacea extracts in the prevention of skin photodamage. Planta Med.1995;61:510-514
[94]Matthias A, Blanchfield J, Penman K, et al. Permeability studies of alkylamides and caffeic acid conjugates from echinacea using a caco-2 cell monolayer model. Journal of clinical pharmacy and therapeutics.2004;29:7-13
[95]Matthias A, Addison R, Agnew L, et al. Comparison of echinacea alkylamide pharmacokinetics between liquid and tablet preparations. Phytomedicine. 2007;14:587-590
[96]Matthias A, Addison R, Penman K, et al. Echinacea alkamide disposition and pharmacokinetics in humans after tablet ingestion. Life sciences.2005;77:2018-2029
[97]Woelkart K, Marth E, Suter A, et al. Bioavailability and pharmacokinetics of echinacea purpurea preparations and their interaction with the immune system. International journal of clinical pharmacology and therapeutics.2006;44:401-408
[98]陈波,潘振球.高效液相色谱法制备菊苣酸标准对照品的研究.实用预防医学.2001；8：15-17
[99]Wang X, Geng Y, Li F, et al. Preparative separation of cichoric acid from echinacea purpurea by ph-zone-refining counter-current chromatography. J Chromatogr A. 2006; 1103:166-169
[100]陈养文.紫锥菊中菊芭酸提取纯化工艺研究.中草药.2004；9995-997
[101]刘轶琛,曾建国,陈波,et al.聚酰胺对紫锥菊提取物中菊苣酸的分离纯化研究.中草药.2007；38：530-533
[102]Perry NB, Burgess EJ, leAnne Glennie V. Echinacea standardization:Analytical methods for phenolic compounds and typical levels in medicinal species. Journal of agricultural and food chemistry.2001;49:1702-1706
[103]Pellati F, Benvenuti S, Melegari M, et al. Variability in the composition of anti-oxidant compounds in echinacea species by hplc. Phytochemical Analysis.2005; 16:77-85
[104]徐度,唐宇伟,朱伟Rp-hplc测定紫锥菊提取物中2种咖啡酸衍生物的含量.中成药.2006；28：1493-1497
[105]Gocan S, Radu L, Hadaruga M. Simultaneous analysis by high performance liquid chromatography of hydrophilic compounds and lipophilic compounds (alkamides) from pharmaceutical preparations ofechinacea purpurea root. Chromatographia. 2003;57:677-679
[106]Molgaard P, Johnsen S, Christensen P, et al. Hplc method validated for the simultaneous analysis of cichoric acid and alkamides in echinacea purpurea plants and products. J Agric Food Chem.2003;51:6922-6933
[107]Cech NB, Eleazer MS, Shoffner LT, et al. High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from< i> echinacea purpurea extracts. Journal of chromatography A.2006; 1103:219-228
[108]Luo XB, Chen B, Yao SZ, et al. Simultaneous analysis of caffeic acid derivatives and alkamides in roots and extracts of< i> echinacea purpurea by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry. Journal of chromatography A.2003;986:73-81
[109]Gotti R, Pomponio R, Bertucci C, et al. Simultaneous analysis of the lipophilic and hydrophilic markers of echinacea plant extracts by capillary electrophoresis. Journal of separation science.2002;25:1079-1086
[110]Mancek B, Kreft S. Determination of cichoric acid content in dried press juice of purple coneflower (echinacea purpurea) with capillary electrophoresis. Talanta. 2005;66:1094-1097
[111]Pomponio R, Gotti R, Hudaib M, et al. Analysis of phenolic acids by micellar electrokinetic chromatography:Application to< i> echinacea purpurea plant extracts. Journal ofchromatographyA.2002;945:239-247
[112]黄劲梅,谢培山.紫锥花及其制剂中菊苣酸的薄层扫描含量测定.中药新药与临床药理,2002,13(1)：33-3.2002；13：33-35
[113]Tsukui A, Kuwano K, Mitamura T. Anthocyanin pigment isolated from purple root of sweetpotato. Nihon Kaseigaku Zasshi.1983;34:153-159
[114]Odake K, Terahara N, Saito N, et al. Chemical structures of two anthocyanins from purple sweet potato, ipomoea batatas. Phytochemistry.1992;31:2127-2130
[115]Goda Y, Shimizu T, Kato Y, et al. Two acylated anthocyanins from purple sweet potato. Phytochemistry.1997;44:183-186
[116]Terahara N, Shimizu T, Kato Y, et al. Six diacylated anthocyanins from the storage roots of purple sweet potato, ipomoea batatas. Bioscience, biotechnology, and biochemistry. 1999;63:1420-1424
[117]姜平平.花色苷类物质抗氧化生理活性的研究[d].2003
[118]Matsui T, Ebuchi S, Kobayashi M, et al. Anti-hyperglycemic effect of diacylated anthocyanin derived from ipomoea batatas cultivar ayamurasaki can be achieved through the a-glucosidase inhibitory action. Journal of agricultural and food chemistry. 2002;50:7244-7248
[119]Yoshimoto M, Okuno S, Yoshinaga M, et al. Antimutagenicity of sweetpotato (ipomoea batatas) roots. Bioscience, biotechnology, and biochemistry.1999;63:537-541
[120]方忠祥,倪元颖.花青素生理功能研究进展.广州食品工业科技.2001；17：60-62
[121]岳静,杰宏筠.紫甘薯红色素体外抑菌性初探.辽宁农业科学.2005：47-47
[122]Suda I, Yamakawa O, Matsugano K, et al. Changes of serum gamma-gtp, got and gpt levels in hepatic function-weakling subjects by ingestion of high anthocyanin sweetpotato juice. Journal of the Japanese Society for Food Science and Technology.1998;45
[123]Suda I, Furuta S, Nishiba Y, et al. Reduction of liver injury induced by carbon tetrachloride in rats administered purple colored sweetpotato juice. Journal of the Japanese Society for Food Science and Technology.1997;44:315-318
[124]Yamakawa O, Suda I, Yoshimoto M. Development and utilization of sweetpotato cultivars with high anthocyanin content. FOODS AND FOOD INGREDIENTS JOURNAL OF JAPAN.1998:69-78
[125]Wang K, Liu Z, Huang JA, et al. Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2008;867:282-286
[126]Gao M, Gu M, Liu CZ. Two-step purification of scutellarin from erigeron breviscapus (vant.) hand. Mazz. By high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2006;838:139-143
[127]Cao X, Tian Y, Zhang T, et al. Separation and purification of isoflavones from pueraria lobata by high-speed counter-current chromatography. J Chromatogr A. 1999;855:709-713
[128]Du Q, Li Z, Ito Y. Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography. Journal of chromatography A. 2001;923:271-274
[129]Peng J, Zhan L, Dong F, et al. A comparative study of chromatographic methods for separating chemical compounds from< i> spiranthes australis(r. Brown) lindl roots. Separation and Purification Technology.2008;59:262-269
[130]孙印石,刘政波,王建华,et a1.高速逆流色谱分离制备陈皮中的黄酮类化合物.色谱.2009；2009
[131]赵淑杰,韩梅,韩忠明,et al.高速逆流色谱分离与鉴定鹿药中黄酮类化合物.分析化学.2009；37：1354-1358
[132]徐渊金.杨梅和桑葚花色苷的提取分离、结构鉴定及其生物活性研究.浙江工商大学.2007
[133]Degenhardt A, Engelhardt UH, Lakenbrink C, et al. Preparative separation of polyphenols from tea by high-speed countercurrent chromatography. JAgric Food Chem. 2000;48:3425-3430
[134]Schwarz M, Wray V, Winterhalter P. Isolation and identification of novel pyranoanthocyanins from black carrot (daucus carota 1.) juice. J Agric Food Chem. 2004;52:5095-5101
[135]胡晓丹.紫苏中次生代谢物的现代提取分离技术及性质研究[d].2008
[136]李佳银,罗晋,李觅路,et al.高速逆流色谱法分离紫锥菊花色苷及其抗氧化性研究.分析测试学报.2012；1
[137]Tang Q, Yang C, Ye W, et al. Preparative isolation and purification of bioactive constituents from< i> aconitum coreanum by high-speed counter-current chromatography coupled with evaporative light scattering detection. Journal of chromatography A.2007;1144:203-207
[138]Liu R, Chu X, Sun A, et al. Preparative isolation and purification of alkaloids from the chinese medicinal herb< i> evodia rutaecarpa(juss.) benth by high-speed counter-current chromatography. Journal of chromatography A.2005; 1074:139-144
[139]Liu Z, Jin Y, Shen P, et al. Separation and purification of verticine and verticinone from< i> bulbus fritillariae thunbergii by high-speed counter-current chromatography coupled with evaporative light scattering detection. Talanta.2007;71:1873-1876
[140]吴三桥,冯自立,李新生,et al.高速逆流色谱法分离纯化延胡索乙素和原阿片碱.中国生化药物杂志.2009；30：89-92
[141]褚建军,颜继忠.制备型高速逆流色谱分离中药中的生物碱.浙江工业大学学报.2006；34
[142]Yang C, Li D, Wan X. Combination of hscce and sephadex lh-20 methods an approach to isolation and purification of the main individual theaflavins from black tea. J Chromatogr B Analyt Technol Biomed Life Sci.2008;861:140-144
[143]Geng Y, Liu J, Lv R, et al. An efficient method for extraction, separation and purification of eugenol from eugenia caryophyllata by supercritical fluid extraction and high-speed counter-current chromatography. Separation and Purification Technology. 2007;57:237-241
[144]Shi S, Huang K, Zhang Y, et al. Purification and identification of antiviral components from laggera pterodonta by high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci.2007;859:119-124
[145]
[146]Becker H, Hsieh WC. Chicoree-saure und deren derivate aus echinacea-arten. Zeitschrift fur Naturforschung. Section C, Biosciences.1985;40:585-587
[147]Yasuda I, Takeya K, Itokawa H. Structures of amides from asiasarum heterotropoides maek. Var. Mandshuricum maek. Chem. Pharm. Bull.1981;29:564-566
[148]Suda I, Oki T, Masuda M, et al. Direct absorption of acylated anthocyanin in purple-fleshed sweet potato into rats. Journal of agricultural and food chemistry. 2002;50:1672-1676
[149]Kano M, Takayanagi T, Harada K, et al. Antioxidative activity of anthocyanins from purple sweet potato, ipomoera batatas cultivar ayamurasaki. Bioscience, biotechnology, and biochemistry.2005;69:979-988
[150]Suda I, Furuta S, Nishiba Y, et al. Hepato-protective activity of purple-colored sweetpotato juice. Sweetpotato Research Front.1997;4
[151]韩永斌,朱洪梅,顾振新,et al.紫甘薯花色苷色素的抑菌作用研究.微生物学通报.2008；35：913-917
[152]Harborne J. Spectral methods of characterizing anthocyanins. Biochemical Journal. 1958;70:22
[153]滕荣伟,周志宏,王德祖,et al.白花刺参中的咖啡酰基奎宁酸成分.波学学杂志.2002；19：167-174
[154]Islam MS, Yoshimoto M, Yahara S, et al. Identification and characterization of foliar polyphenolic composition in sweetpotato (ipomoea batatas 1.) genotypes. Journal of agricultural and food chemistry.2002;50:3718-3722
[155]Von Itzstein M. The war against influenza:Discovery and development of sialidase inhibitors. Nature Reviews Drug Discovery.2007;6:967-974
[156]Ohuchi M, Asaoka N, Sakai T, et al. Roles of neuraminidase in the initial stage of influenza virus infection. Microbes and infection.2006;8:1287-1293
[157]Moscona A. Medical management of influenza infection. Annu. Rev. Med. 2008;59:397-413
[158]Garman E, Laver G.Controlling influenza by inhibiting the viruss neuraminidase. Current drug targets.2004;5:119-136
[159]Nayak DP, Reich1 U. Neuraminidase activity assays for monitoring mdck cell culture derived influenza virus. Journal of virological methods.2004;122:9-15
[160]Ryu YB, Curtis-Long MJ, Kim JH, et al. Pterocarpans and flavanones from sophora flavescens displaying potent neuraminidase inhibition. Bioorg Med Chem Lett. 2008; 18:6046-6049
[161]高雷,章天,陈鸿珊.黄芩甙对流感病毒神经氨酸酶的抑制作用.中国现代医生.2007；45：46-46
[162]Cuzzocrea S, Riley DP, Caputi AP, et al. Antioxidant therapy:A new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacological reviews.2001;53:135-159
[163]Jun PZ, Yuan C, Mei Z. The effect of polysaccharide krestin on gpx gene expression in macrophages [j]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA.1999;3
[164]赵艳红,李建科,李国秀.天然抗氧化物体外活性评价方法的优选与优化.食品科学.2008；29：64-69
[165]Cotelle N, Bernier JL, Catteau JP, et al. Antioxidant properties of hydroxy-flavones. Free Radical Biology and Medicine.1996;20:35-43
[166]Amarowicz R, Pegg R, Rahimi-Moghaddam P, et al. Free-radical scavenging capacity and antioxidant activity of selected plant species from the canadian prairies. Food Chemistry.2004;84:551-562
[167]田卉,马建慧,张梅,et al.石榴皮的体外抗脂质过氧化作用.中药药理与临床.2009；25：69-70
[168]董欢欢,曹树稳,余燕影.半枝莲,白花蛇舌草及其药对提取物抗氧化及清除自由基活性.无然产物研究与开发.2008；20：782-786
[169]Ningappa MB, Dinesha R, Srinivas L. Antioxidant and free radical scavenging activities of polyphenol-enriched curry leaf (murraya koenigii 1.) extracts. Food Chemistry. 2008; 106:720-728
[170]孙庆雷,王晓,刘建华,et al.黄酮类化合物抗氧化反应性的构效关系.食品科学.2005；26：69-72
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