后交叉韧带功能分束及其断裂对外侧胫骨平台影响的实验研究
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摘要
后交叉韧带(Posterior cruciate ligament, PCL)是膝关节主要的稳定结构之一,其主要功能是防止胫骨后移,其次参与膝内外翻和旋转的调节。本实验拟对PCL不同束在不同角度的应变进行测量,探讨各束的生物力学功能及各束的内在联系；采用生物力学测定的方法,观察PCL完整、完全断裂和部分断裂对外侧胫骨平台各部位应变的影响；并运用组织学方法,观察PCL完全断裂后外侧胫骨平台软骨组织形态学的变化,同时检测基质金属蛋白酶13(matrix metalloproteinase-13, MMP-13)、基质金属蛋白酶抑制剂1(Tisse inhibitor-1 of matrix metalloproteinase, TIMP-1)的表达,以了解其在外侧胫骨平台软骨退变中的作用机制,为PCL损伤是否对外侧胫骨平台产生影响提供理论依据。
第一章后交叉韧带功能性分束的生物力学实验研究
目的探讨PCL的各纤维束的生物力学特性及其相应的功能及分类,为后续实验及临床工作提供理论依据。
方法12具新鲜成人尸体膝关节标本,依据PCL在膝关节中的空间位置将PCL分为：前内侧区纤维束(前内侧束)、前外侧区纤维束(前外侧束)、后内侧区纤维束(后内侧束)、后外侧区纤维束(后外侧束)。在膝关节标本上施加1000N轴向载荷下,分别测试膝关节0°、30°、60°、90°状态下测PCL各纤维束应变。比较各束应变的差异,采用样品聚类方法进行分类
结果1、膝关节0°位,PCL后内侧束、后外侧束应变均大于前内侧束、前外侧束应变,有显著性差异,P<0.05；前内侧束与前外侧束应变无显著性差异,P>0.05；后外侧束与后内侧束应变无显著性差异,P>0.05°30°、60°和90°位,PCL前内侧束、前外侧束应变均大于后内侧束、后外侧束应变,有显著性差异,P<0.05；后内侧束与后外侧束应变比较无显著性差异,P>0.05；前内侧束与前外侧束应变比较无显著性差异,P>0.05。2、前外侧束与前内侧束应变变化一致,依序0°、30°、60°、90°逐渐增大,均有显著性差异,P<0.05。3、聚类分析：后内侧束与后外侧束归为一类,前内侧束与前外侧束归为一类。
结论(1)PCL止点各区纤维束在膝关节屈伸过程中始终处于张力状态。
(2)后交叉韧带内存在不同功能的纤维束,前内侧区纤维束和前外侧区纤维束可能在维持膝关节屈曲稳定性起作用,后内侧区纤维束和后外侧区纤维束可能在维持膝关节伸直稳定性起作用。
(3)结合聚类分析结果,聚类两束在PCL股骨止点一内一外的解剖特点,PCL可分为后内侧束、前外侧束。
第二章后交叉韧带断裂对外侧胫骨平台生物力学的影响
目的探讨PCL断裂对外侧胫骨平台生物力学的影响。
方法新鲜正常成人膝关节标本12具作为PCL完整组,在200N、400N、600N、800N、1000N载荷下,测试膝关节0°、30°、60°、90°位外侧胫骨平台前部、体部和后部的应变,测试完毕后随机将标本造模成前外侧束(anterolateral bundle, ALB)断裂组和后内侧束(posteromedial bundle, PMB)断裂组,各6具,在上述条件下测试,最后再将12具标本的PCL完全切断进行测试。
结果1.膝关节0°位,在200N和400N载荷下,外侧胫骨平台前部、体部和后部应变在PCL完整组和ALB切断组无明显差异,全断组和PMB切断组无明显差异,P>0.05；在600N、800N和1000N时,各实验组之间应变绝对值有显著差异,P<0.05,在前部和体部全断组>PMB切断组>ALB切断组>完整组；在后部,全断组全切断组和ALB切断组无明显差异,P>0.05；在600N、800N和1000N时,各实验组之间应变有显著差异,P<0.05,在前、体部,其绝对值表现为全断组>ALB切断组>PMB切断组>完整组,在后部,其绝对值表现为全断组ALB切断组>PMB切断组>完整组,在后部,完整组、PMB切断组为压应变,其绝对值表现为完整组>PMB切断组,全断组、ALB切断组为拉应变,其绝对值表现为全断组>ALB切断组。
结论1.PCL断裂,在不同载荷和角度下,会引起外侧胫骨平台力学负荷分布异常。2.在200N和400N载荷下,ALB切断在膝关节0°位和PMB切断在膝关节屈曲30°位对外侧胫骨平台各部的应变均无影响。3.在600N、800N和1000N载荷下,ALB切断在膝关节0°位和PMB切断在膝关节屈曲30°位对外侧胫骨平台各部的应变均有影响。4.PCL完全性断裂会引起膝关节各个角度和载荷下外侧胫骨平台各部力学负荷异常。5.在各实验载荷下,ALB切断在屈膝30°60°、90°位和PMB切断在膝0°位对外侧胫骨平台各部的应变均有影响。
第三章后交叉韧带断裂外侧胫骨平台退变组织学研究
目的探讨PCL断裂对外侧胫骨平台组织学的影响。
方法48只膝关节配对为实验侧和对照侧,实验侧行PCL切断造模,造模后第4、8、16、24周各随机处死12只,行外侧胫骨平台大体观察,并进行HE染色,免疫组化检测基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13).基质金属蛋白酶抑制剂1(Tisse inhibitor-1 of matrix metalloproteinasel,TIMP-1)表达。
结果1.大体观察,随时间延长,实验组外侧胫骨平台软骨出现磨损,呈灰黄色,弹性差,骨赘形成。2.组织学观察,随时间延长,胫骨平台软骨纤维化,细胞排列紊乱,簇聚细胞出现频率增加。3.实验组MMP-13、TIMP-1表达均高于对照组,有显著性差异,P<0.05。4.实验组MMP-13表达阳性率第4、8、16周逐渐升高,24周下降,各组比较有显著性差异,P<0.05。5.实验组TIMP-1表达阳性率第4、8、16周逐渐升高,24周下降,各组比较有显著性差异,P<0.05。
结论1.PCL断裂会引起膝关节外侧胫骨平台软骨退行性变,且该退变随着时间的推移逐渐加重。2. MMP-13与TIMP-1在PCL断裂膝关节外侧胫骨平台中的表达呈现先高后低的变化规律,造成MMP-13与TIMP-1的失衡,加速软骨退变。3. MMP-13与TIMP-1表达增高提示MMP-13与TIMP-1可能参与了PCL断裂后外侧胫骨平台软骨的退变过程。
The posterior cruciate ligament (PCL) is a stable structure whose main role is controlling the posteodisplacement of tibial and regulating the rotation of tibial as well as the varus and valgus of knee joint partly. In this study, straining of PCL bundles is tested under different angle so as to investigate the biomechanical function of the PCL bundles and their internal correlation.Straining changes in several sites of the lateral tibial plateau by PCL injury is observed through biomechanics approach; histological changes of lateral tibial plateau cartilage and expression of MMP-13 and TIMP-1 in lateral tibial plateau cartilage are observed so as to investigate the function of MMP-13 and TIMP-1 in degeneration of lateral tibial plateau cartilage;It cound for us to provide theoretical evidence whether deficiency of PCL effects on the lateral tibial plateau.
Chapter I The biomechanical study of PCL bundles' functional classification
OBJECTIVE To investigate the biomechanical function of the PCL bundles and their internal correlation and provide biomechanical evidence for the later experiments and clinical therapy.
METHOD 12 fresh cadaveric knees from adult human beings were used. Selected the bilateral anterior part of the PCL tibial insertion and the bilateral posterior part of the PCL femoral insertion as the measure parts correspond to:anteromedial area fiber bundle (anteromedial bundle),anterolateral area fiber bundle (anterolateral bundle), posteromedial area bundle (posteromedial bundle) and posterolateral area bundle (posterolateral bundle). The strain on the PCL bundles was measured when the knees were applied with 1000N axial loading force in 0°,30°,60°and 90°positions. The bundles were functional classified by cluster analysis.
RESULT 1.In 0°position, the strain on the posteromedial and posterolateral bundles was significantly larger than the anteromedial and anterolateral bundles (P<0.05), There were no significant diference between the anteromedial and anterolateral bundles (P>0.05) and so did the posteromedial and posterolateral bundles (P>0.05); In 30°,60°and 90°position, the strain on the anteromedial and anterolateral bundles was significantly larger than the posteromedial and posterolateral bundles (P<0.05), There were no significant difference between the posteromedial and posterolateral bundles (P>0.05) and so did the anteromedial and anterolateral bundles (P>0.05).2.The strain on the anterolateral and anteromedial bundles was increased by degrees when the knee flexed 0°,30°,60°and 90°, and all the difference were significant (P<0.05).3.Cluster analysis classfied the anteromedial bundle and the anterolateral bundle as one class and the posteromedial and posterolateral bundles as the other class.
CONCLUSION 1.The anteromedial area and anterolateral area fiber bundles cound mainly maintain stability of the knee in flexed positions. 2.The posteromedial area and posterolateral area fiber bundles cound mainly maintain stability of the knee in extended positions. 3. Combined with the cluster analysis results, PCL may be classified into the anterolateral functional fiber bundle and the posteromedial functional fiber bundle.
ChapterⅡThe Effect of PCL Rupture on the Biomechanics of Lateral Tibial Plateau
OBJECTIVE To investigate the biomechanical influences of partial and total PCL rupture on the lateral tibial plateau.
METHOD The straining of lateral tibial plateau anterior part, caudomedial part and posterior part in all fresh normal adult knee joint specimens including 12 specimens of complete PCL,6 of anterolateral bundle(ALB) rupture,6 of posteromedial bundle(PMB) rupture and 12 of complete rupture PCL are tested when the knee joints loaded 200N,400N,600N,800N and 1000N at 0°,30°,60°and 90°of flexion.
RESULT 1.The straining of lateral tibial plateau anterior part, caudomedial part and posterior part shows no significant difference between PMB rupture group and PCL complete rupture group,complete PCL group and ALB rupture group under the load of 200N and 400N at 0°of flexion,P>0.05; significant difference is showed among all the groups under the loads of 600N,800N and 1000N at 0°of flexion,P<0.05; under the same load, the correlation of the absolute value of straining in every group of lateral tibial plateau. anterior part and caudomedial part increases in this way of PCL complete rupture group> PMB rupture group>ALB rupture group> complete PCL group,and of posterior part decreases in this way of PCL complete rupture group< PMB rupture group0.05; significant difference is showed among all the groups under the load of 600N,800N and 1000N at 30°of flexion,P< 0.05; under the same load,the correlation of the absolute value of straining in anterior part and caudomedial part increases in this way of PCL complete rupture group> ALB rupture group> PMB rupture group> complete PCL group, and of posterior part decreases in this way of PCL complete rupture group< ALB rupture group ALB rupture group> PMB rupture group> complete PCL group, there is significant difference among straining of all the groups of posterior part. Those of PCL complete rupture group and ALB rupture group are tebsile straining and PCL complete rupture group> ALB rupture group. Those of complete PCL group and PMB rupture group are compressive straining and complete PCL group> PMB rupture group.
CONCLUSION 1.PCL complete rupture is likely to cause abnormal straining in all regions of lateral tibial plateau under different load and angle of flexion.2. Under the load of 200N and 400N, ALB rupture at 0°of flexion and PMB rupture at 30°of flexion cause no effect on the straining in all regions of lateral tibial plateau.3. Under the load of 600N,800N and 1000N, ALB rupture at 0°of flexion and PMB rupture at 30°of flexion cause apparently effect on the straining in all regions of the lateral tibial plateau.4. Abnormal straining in all regions of lateral tibial plateau can be caused by PCL complete rupture under different angle of flexion 5. Abnormal straining in all regions of the lateral tibial plateau can be caused by PMB rupture at 0°of flexion and ALB rupture at 30°,60°and 90°of flexion under different load.
ChapterⅢThe Research of Histology about Degeneration of Lateral Tibial Plateau after Rupture of PCL
OBJECTIVE Study the histological influence on lateral tibial plateau which was caused by rupture of PCL
METHOD Lateral tibial plateau of 48 rabbits matched mode pairs both in experiment side which PCL were transacted and control side, at the 4st,8rd,16th and 24th week,execute 12 rabbits randomly,then to observe lateral tibial plateau in general and through HE staining and immunohistochemistry staining,detect the expression of matrix metalloproteinase-13 (MMP-13)and Tisse inhibitor-1 of matrix metalloproteinasel (TIMP-1).
RESULT 1.The observation in general showed that along with the increase of the time lateral tibial plateau of experiment group gradually abrasion, presenting isabeling, bad elasticity and formation of osteophytes.2.Along with the increase of the time the observation of histology shows fibering of lateral tibial plateau,meanwhile,there are abnormal cell disposition and increasingly clustered cell.3.expression of MMP-13 and TIMP-1 is higher in experimental group than that in control group,showing significant difference, P<0.05.4.In experimental group, expression of MMP-13 is increasing gradually at the 4rd,8st and 16th week,then decreasing at 24th week,showing significant difference, P< 0.05.5.In experimental group,expression of TIMP-1 is increasing gradually at the 4rd,8st and 16th week, then decreasing at 24th week, showing significant difference, P< 0.05.
CONCLUSION 1. Rupture of PCL could cause the degeneration of the lateral tibial platea,As time go up.this condition will be aggravated.2.A regularity of expression of TIMP-1 and MMP13 is displayed that the expression is increasing in primal stage and decreasing in advanced stage after rupture of PCL,degeneration of the cartilage is speeding up because of unbalance between MMP-13 and TIMP-1.3.It is a important role for MMP-13 and TIMP-1 to participate in the course of degeneration of the lateral tibial plateau cartilage.
第一章后交叉韧带功能性分束的生物力学实验研究
目的探讨PCL的各纤维束的生物力学特性及其相应的功能及分类,为后续实验及临床工作提供理论依据。
方法12具新鲜成人尸体膝关节标本,依据PCL在膝关节中的空间位置将PCL分为：前内侧区纤维束(前内侧束)、前外侧区纤维束(前外侧束)、后内侧区纤维束(后内侧束)、后外侧区纤维束(后外侧束)。在膝关节标本上施加1000N轴向载荷下,分别测试膝关节0°、30°、60°、90°状态下测PCL各纤维束应变。比较各束应变的差异,采用样品聚类方法进行分类
结果1、膝关节0°位,PCL后内侧束、后外侧束应变均大于前内侧束、前外侧束应变,有显著性差异,P<0.05；前内侧束与前外侧束应变无显著性差异,P>0.05；后外侧束与后内侧束应变无显著性差异,P>0.05°30°、60°和90°位,PCL前内侧束、前外侧束应变均大于后内侧束、后外侧束应变,有显著性差异,P<0.05；后内侧束与后外侧束应变比较无显著性差异,P>0.05；前内侧束与前外侧束应变比较无显著性差异,P>0.05。2、前外侧束与前内侧束应变变化一致,依序0°、30°、60°、90°逐渐增大,均有显著性差异,P<0.05。3、聚类分析：后内侧束与后外侧束归为一类,前内侧束与前外侧束归为一类。
结论(1)PCL止点各区纤维束在膝关节屈伸过程中始终处于张力状态。
(2)后交叉韧带内存在不同功能的纤维束,前内侧区纤维束和前外侧区纤维束可能在维持膝关节屈曲稳定性起作用,后内侧区纤维束和后外侧区纤维束可能在维持膝关节伸直稳定性起作用。
(3)结合聚类分析结果,聚类两束在PCL股骨止点一内一外的解剖特点,PCL可分为后内侧束、前外侧束。
第二章后交叉韧带断裂对外侧胫骨平台生物力学的影响
目的探讨PCL断裂对外侧胫骨平台生物力学的影响。
方法新鲜正常成人膝关节标本12具作为PCL完整组,在200N、400N、600N、800N、1000N载荷下,测试膝关节0°、30°、60°、90°位外侧胫骨平台前部、体部和后部的应变,测试完毕后随机将标本造模成前外侧束(anterolateral bundle, ALB)断裂组和后内侧束(posteromedial bundle, PMB)断裂组,各6具,在上述条件下测试,最后再将12具标本的PCL完全切断进行测试。
结果1.膝关节0°位,在200N和400N载荷下,外侧胫骨平台前部、体部和后部应变在PCL完整组和ALB切断组无明显差异,全断组和PMB切断组无明显差异,P>0.05；在600N、800N和1000N时,各实验组之间应变绝对值有显著差异,P<0.05,在前部和体部全断组>PMB切断组>ALB切断组>完整组；在后部,全断组
结论1.PCL断裂,在不同载荷和角度下,会引起外侧胫骨平台力学负荷分布异常。2.在200N和400N载荷下,ALB切断在膝关节0°位和PMB切断在膝关节屈曲30°位对外侧胫骨平台各部的应变均无影响。3.在600N、800N和1000N载荷下,ALB切断在膝关节0°位和PMB切断在膝关节屈曲30°位对外侧胫骨平台各部的应变均有影响。4.PCL完全性断裂会引起膝关节各个角度和载荷下外侧胫骨平台各部力学负荷异常。5.在各实验载荷下,ALB切断在屈膝30°60°、90°位和PMB切断在膝0°位对外侧胫骨平台各部的应变均有影响。
第三章后交叉韧带断裂外侧胫骨平台退变组织学研究
目的探讨PCL断裂对外侧胫骨平台组织学的影响。
方法48只膝关节配对为实验侧和对照侧,实验侧行PCL切断造模,造模后第4、8、16、24周各随机处死12只,行外侧胫骨平台大体观察,并进行HE染色,免疫组化检测基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13).基质金属蛋白酶抑制剂1(Tisse inhibitor-1 of matrix metalloproteinasel,TIMP-1)表达。
结果1.大体观察,随时间延长,实验组外侧胫骨平台软骨出现磨损,呈灰黄色,弹性差,骨赘形成。2.组织学观察,随时间延长,胫骨平台软骨纤维化,细胞排列紊乱,簇聚细胞出现频率增加。3.实验组MMP-13、TIMP-1表达均高于对照组,有显著性差异,P<0.05。4.实验组MMP-13表达阳性率第4、8、16周逐渐升高,24周下降,各组比较有显著性差异,P<0.05。5.实验组TIMP-1表达阳性率第4、8、16周逐渐升高,24周下降,各组比较有显著性差异,P<0.05。
结论1.PCL断裂会引起膝关节外侧胫骨平台软骨退行性变,且该退变随着时间的推移逐渐加重。2. MMP-13与TIMP-1在PCL断裂膝关节外侧胫骨平台中的表达呈现先高后低的变化规律,造成MMP-13与TIMP-1的失衡,加速软骨退变。3. MMP-13与TIMP-1表达增高提示MMP-13与TIMP-1可能参与了PCL断裂后外侧胫骨平台软骨的退变过程。
The posterior cruciate ligament (PCL) is a stable structure whose main role is controlling the posteodisplacement of tibial and regulating the rotation of tibial as well as the varus and valgus of knee joint partly. In this study, straining of PCL bundles is tested under different angle so as to investigate the biomechanical function of the PCL bundles and their internal correlation.Straining changes in several sites of the lateral tibial plateau by PCL injury is observed through biomechanics approach; histological changes of lateral tibial plateau cartilage and expression of MMP-13 and TIMP-1 in lateral tibial plateau cartilage are observed so as to investigate the function of MMP-13 and TIMP-1 in degeneration of lateral tibial plateau cartilage;It cound for us to provide theoretical evidence whether deficiency of PCL effects on the lateral tibial plateau.
Chapter I The biomechanical study of PCL bundles' functional classification
OBJECTIVE To investigate the biomechanical function of the PCL bundles and their internal correlation and provide biomechanical evidence for the later experiments and clinical therapy.
METHOD 12 fresh cadaveric knees from adult human beings were used. Selected the bilateral anterior part of the PCL tibial insertion and the bilateral posterior part of the PCL femoral insertion as the measure parts correspond to:anteromedial area fiber bundle (anteromedial bundle),anterolateral area fiber bundle (anterolateral bundle), posteromedial area bundle (posteromedial bundle) and posterolateral area bundle (posterolateral bundle). The strain on the PCL bundles was measured when the knees were applied with 1000N axial loading force in 0°,30°,60°and 90°positions. The bundles were functional classified by cluster analysis.
RESULT 1.In 0°position, the strain on the posteromedial and posterolateral bundles was significantly larger than the anteromedial and anterolateral bundles (P<0.05), There were no significant diference between the anteromedial and anterolateral bundles (P>0.05) and so did the posteromedial and posterolateral bundles (P>0.05); In 30°,60°and 90°position, the strain on the anteromedial and anterolateral bundles was significantly larger than the posteromedial and posterolateral bundles (P<0.05), There were no significant difference between the posteromedial and posterolateral bundles (P>0.05) and so did the anteromedial and anterolateral bundles (P>0.05).2.The strain on the anterolateral and anteromedial bundles was increased by degrees when the knee flexed 0°,30°,60°and 90°, and all the difference were significant (P<0.05).3.Cluster analysis classfied the anteromedial bundle and the anterolateral bundle as one class and the posteromedial and posterolateral bundles as the other class.
CONCLUSION 1.The anteromedial area and anterolateral area fiber bundles cound mainly maintain stability of the knee in flexed positions. 2.The posteromedial area and posterolateral area fiber bundles cound mainly maintain stability of the knee in extended positions. 3. Combined with the cluster analysis results, PCL may be classified into the anterolateral functional fiber bundle and the posteromedial functional fiber bundle.
ChapterⅡThe Effect of PCL Rupture on the Biomechanics of Lateral Tibial Plateau
OBJECTIVE To investigate the biomechanical influences of partial and total PCL rupture on the lateral tibial plateau.
METHOD The straining of lateral tibial plateau anterior part, caudomedial part and posterior part in all fresh normal adult knee joint specimens including 12 specimens of complete PCL,6 of anterolateral bundle(ALB) rupture,6 of posteromedial bundle(PMB) rupture and 12 of complete rupture PCL are tested when the knee joints loaded 200N,400N,600N,800N and 1000N at 0°,30°,60°and 90°of flexion.
RESULT 1.The straining of lateral tibial plateau anterior part, caudomedial part and posterior part shows no significant difference between PMB rupture group and PCL complete rupture group,complete PCL group and ALB rupture group under the load of 200N and 400N at 0°of flexion,P>0.05; significant difference is showed among all the groups under the loads of 600N,800N and 1000N at 0°of flexion,P<0.05; under the same load, the correlation of the absolute value of straining in every group of lateral tibial plateau. anterior part and caudomedial part increases in this way of PCL complete rupture group> PMB rupture group>ALB rupture group> complete PCL group,and of posterior part decreases in this way of PCL complete rupture group< PMB rupture group
CONCLUSION 1.PCL complete rupture is likely to cause abnormal straining in all regions of lateral tibial plateau under different load and angle of flexion.2. Under the load of 200N and 400N, ALB rupture at 0°of flexion and PMB rupture at 30°of flexion cause no effect on the straining in all regions of lateral tibial plateau.3. Under the load of 600N,800N and 1000N, ALB rupture at 0°of flexion and PMB rupture at 30°of flexion cause apparently effect on the straining in all regions of the lateral tibial plateau.4. Abnormal straining in all regions of lateral tibial plateau can be caused by PCL complete rupture under different angle of flexion 5. Abnormal straining in all regions of the lateral tibial plateau can be caused by PMB rupture at 0°of flexion and ALB rupture at 30°,60°and 90°of flexion under different load.
ChapterⅢThe Research of Histology about Degeneration of Lateral Tibial Plateau after Rupture of PCL
OBJECTIVE Study the histological influence on lateral tibial plateau which was caused by rupture of PCL
METHOD Lateral tibial plateau of 48 rabbits matched mode pairs both in experiment side which PCL were transacted and control side, at the 4st,8rd,16th and 24th week,execute 12 rabbits randomly,then to observe lateral tibial plateau in general and through HE staining and immunohistochemistry staining,detect the expression of matrix metalloproteinase-13 (MMP-13)and Tisse inhibitor-1 of matrix metalloproteinasel (TIMP-1).
RESULT 1.The observation in general showed that along with the increase of the time lateral tibial plateau of experiment group gradually abrasion, presenting isabeling, bad elasticity and formation of osteophytes.2.Along with the increase of the time the observation of histology shows fibering of lateral tibial plateau,meanwhile,there are abnormal cell disposition and increasingly clustered cell.3.expression of MMP-13 and TIMP-1 is higher in experimental group than that in control group,showing significant difference, P<0.05.4.In experimental group, expression of MMP-13 is increasing gradually at the 4rd,8st and 16th week,then decreasing at 24th week,showing significant difference, P< 0.05.5.In experimental group,expression of TIMP-1 is increasing gradually at the 4rd,8st and 16th week, then decreasing at 24th week, showing significant difference, P< 0.05.
CONCLUSION 1. Rupture of PCL could cause the degeneration of the lateral tibial platea,As time go up.this condition will be aggravated.2.A regularity of expression of TIMP-1 and MMP13 is displayed that the expression is increasing in primal stage and decreasing in advanced stage after rupture of PCL,degeneration of the cartilage is speeding up because of unbalance between MMP-13 and TIMP-1.3.It is a important role for MMP-13 and TIMP-1 to participate in the course of degeneration of the lateral tibial plateau cartilage.
引文
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