减之胶囊对肥胖大鼠减肥降脂作用的实验观察及其机制研究
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摘要
目的：研究中药复方制剂减之胶囊对肥胖大鼠的减肥降脂作用及其作用机制。
方法：1．肥胖大鼠模型的建立：雄性SD大鼠90只，50g-70g，按体重随机分为模型对照组(10只)，每日喂饲普通饲料；造模组(80只)，每日喂饲高脂饲料。两组大鼠每日饮食量相同，自由饮水，每两周称体重一次，持续8周。根据造模组大鼠体重比对照组大鼠体重增加20％作为肥胖大鼠，共选出75只肥胖大鼠。
2．减肥实验：将75只造模成功的肥胖大鼠按体重和血清胆固醇随机分成五组，每组15只。第一组为空白对照组(normal control group，Con)，每只2ml／d蒸馏水灌胃；第二组为阳性对照组(Orlistat group，Orl)，给与奥利司他60mg·kg~(-1)·d~(-1)灌胃；其余三组为实验组：低剂量实验组(low-dosage experiment group of Jianzhi capsules，JZH1)，中剂量实验组(medium-dosage experiment group of Jianzhi capsules，JZH2)，高剂量实验组(high-dosage experiment group of Jianzhi capsules，JZH3)，分别以减之胶囊165mg·kg~(-1)·d~(-1)、250mg·kg~(-1)·d~(-1)、330mg·kg~(1-)·d~(-1)灌胃，减肥实验共40天，在减肥实验过程中，继续给予高脂饲料喂饲。其间每十天称体重一次；分别在减肥实验前，减肥实验第20天和第40天经尾部抽取各组大鼠血液，测定血清甘油三酯，胆固醇和高密度脂蛋白的浓度，减肥实验第40天同时测定空腹血清卵磷脂胆固醇酯酰转移酶(LCAT)，脂蛋白脂酶(LPL)，肝脂酶(HL)活性；实验结束前用乙醚麻醉，测量体长和尾长，计算Lees指数；在末次给药后24小时颈动脉放血致死，
浙江大学硕士学位论文
取腹部，肾周围及生殖器周围脂肪组织称重，其中生殖器周围脂肪组织经固定制片后，观察
脂肪细胞的大小和数目。
3.减之胶囊在体外对脂肪酶的抑制作用实验研究:
脂肪酶活性测定:取o.lml生理盐水，0、lml脂肪酶液[[Lipase]。v。，a.，=47.56Ulmll，加
37℃橄榄油乳剂3ml混合，立即置于紫外分光光度计340nm测定吸收值，连续记录
lomin，Zomin，30min，40min，50min，6omin的吸光度。同法测量o.lml减之胶囊(o.lm留ml)，o.lml
脂肪酶液，加橄榄油3ml时的酶活性。
不同剂量减之胶囊对脂肪酶活性影响:配制六个不同剂量的减之胶囊(m留ml):o、0.05、
0.1、0.15、0.2、0.25。测量不同剂量减之胶囊对脂肪酶活性的影响，方法如卜:将不同剂量
减之胶囊0.1 ml分别与0.lml脂肪酶液[[Lipase]。，r=47.56U/ml]，橄榄油乳剂3ml在37，C条夕I
下保温40分钟，在紫外分光光度计340nm处测定吸收值。
结果:
1.减之胶囊对肥胖大鼠体重的影响:减肥实验开始时，各组大鼠体重无明显区别，相
互比较无显著差异性(P>0.05).减肥实验第20天和第30天，实验组大鼠体重明显低于空
白对照组，相互比较有差异显著性(P<0.05)，与阳性对照组比较无显著性差异(P>0.05)。
实验第40天，低剂量实验组和中剂量实验组大鼠体重明显低于空白对照组，相互比较有差
异显著性(P<0.05)，与阳性对照组比较无显著性差异(P>0.05);高剂量实验组的大鼠体重
更明显低于空白对照组，两者比较有差异显著性(P<0.01);高剂量实验组的大鼠体重也明
显低于低、中剂量实验组，相互比较有差异显著性(P<0.05)，与阳性对照组比较也有差异
显著性(P<0.05)。
2.减之胶囊对肥胖大鼠内脏脂肪组织重量的影响:低、中、高剂量实验组的腹脂、肾
周脂、生殖器周围脂肪的重量均明显低于空白对照组，相互比较有差异显著性(P<0.05)，
与阳性对照组比较无显著差异性(P>0.05);实验组各组间无差异显著性(P>0.05)。
3.减之胶囊对肥胖大鼠】未。指数的影响:各实验组的Lees指数均明显低于空白对照
组.两者比较有显著差异性(P<0 .05)，与阳性对照组比较无显著差异性。其中高剂量实验
组与空白对照组的差异更大(P<0 .05)。实验组各组间无差异性(P>0.05)。
4.减之胶囊对肥胖大鼠脂肪组织形态的影响:实验各组的脂肪细胞直径均明显变小，
与空白对照组比较，有显著性差异(P劝.05)，与阳性对照组比较无差异显著性(P>0.05)，
不同剂量的减之胶囊对脂肪细胞大小影响不明显(P>0.05).各实验组脂肪细胞数目(每高
3
浙江大学硕士学位论文
倍视野)明显较空白对照组多，相互比较有显著性差异(P<0.05)，与阳性对照组比较无显
著性差异(P>0.05)，实验组组间比较无明显差异(P>0.05)。
5.减之胶囊对肥胖大鼠血清胆固醉(TC)、血清甘油三酣(TG)、血清高密度脂蛋白
(HDL)的影响:减肥实验开始时，各组大鼠血清胆固醇(TC)浓度无明显差异(P>0.05)。
实验结束时(第40天)减之胶囊实验组大鼠血清胆固醇(TC)明显低于空白对照组，相互
比较差别有显著性(P<0.05);减肥实验开始时，各组大鼠血清甘油三酷(TG)浓度无明显
差异(P>0.OS)，实验中期(20天)和实验结束时(40)，实验组和阳性对照组人鼠血清甘
油二酷(TG)浓度明显低于空白对照组，相互比较有显著性差异(P<0.05)。减肥实验开始
和结束时，各组大鼠血清高密度脂蛋白(HDL)的浓度无明显差异(
Objective: To assess the anti-obesity effect and mechanism of Jianzhi capsules on the obese rats induced by high fat diet.
Methods: Obesity was induced by high fat diet. Male SD strain rats (50g-70g),were randomly divided into control group(10 rats) and model group(80 rats),being given normal diet and high fat diet respectively for 8 weeks. Each rat had the same volume of diet per day and was weighed every two weeks.
After the models were prepared successfully, they were randomly divided into five groups:the normal control group (Con), the Orlistat control group (Orl), the low-dosage experiment group of Jianzhi capsules (JZHl),the medium-dosage experiment group of Jianzhi capsules (JZH2), the high-dosage experiment group of Jianzhi capsules (JZH3) (15 rats per group). The experiment groups were administered with Jianzhi capsules at the dosages of 165mg kg-1 d-1, 250mg kg-1d-1, 330mg kg-1d-1, while the Orlistat control group with Orlistat at the dosage of 60mg kg-1d-1. According to the dosage, the concentration of Jianzhi capsules and Orlistat of 2ml per day for one rat was prepared, while the normal control group with the same volume of distilled water. The experiment lasted 40 days. During the course ,the body weights were measured every ten days; The fast blood cholesterol (TC), triglycerides(TG),high-density lipoprotein (HDL),were tested at the beginning (the early stage), 20th day (the mid stage) and 40th day (the la
te stage); And at the late stage, the activity of the fast blood lecithin cholesterol acyltransferase (LCAT), lipoprotein lipase (LPL), hepatic lipase (HL) were also determined; By the end of the test, the rats were killed
and their fat tissues in the abdomenon,perirenal and perigenital were removed and weighed; After
the fat tissues in the perigenital were fixed and stained, the size and number of their fat cells were studies.
The study of inhibition of Jianzhi capsules on lipase in vitro: 0.lml Jianzhi capsules ,0.1 ml lipase and 3ml olive oil emulsion were mixed and measured immediately at the time of 10min,20min,30min,40min,50min,60min. A curve of product-time was obtained.Effect of six different dosages of Jianzhi capsules on the activity level of lipase were measured. The dosage of Jianzhi capsules (mg/ml) were: 0, 0.05, 0.1, 0.15, 0.2, 0.25. The method was the same as the above.At the time of 40 minutes, six different figures were obtained.
Results:
1. Effect of Jianzhi capsules on the body weight in the obese rats:
There were no differences in weights between the control groups and Jianzhi capsules groups before administration (p>0.05). After given Jianzhi capsules for 20 days and 30 days ,the weights in Jianzhi capsules groups were reduced significantly compared with the normal control group (P<0.05), however.there was no difference with the Orlistat control group.(P> 0.05). At the time of 40 days, the level of weights in the JZH1 and JZH2 was significantly decreased compared with the normal control group (P<0.05), however ,there was no difference with the Orlistat control group (P>0.05), and the level of weights in the JZH3 was obviously reduced compared with any other group (P<0.05).
2. Effect of Jianzhi capsules on the weights of fat tissues in the abdomnon, perirenal, perigenital in the obese rats:
The weights of fat tissues in Jianzhi groups were significantly reduced compared with those of the normal control group(P<0.05) while there was no difference with the Orlistat control group(P>0.05).
3. Effect of Jianzhi capsules on the Lees Indexes in the obese rats:
The Lees Indexes in the Jianzhi groups were significantly decreased compared with those of the normal control group(P<0.05), while there was no difference with the Orlistat control group(P>0.05).There were on significantly difference among the test groups.
4. Effect of Jianzhi capsules on the size and number of .fat cells in the perigenital:
The size of fat cells in the Jianhzi groups was significantly reduced compared with that in
normal control group (P<0.05) while there
方法：1．肥胖大鼠模型的建立：雄性SD大鼠90只，50g-70g，按体重随机分为模型对照组(10只)，每日喂饲普通饲料；造模组(80只)，每日喂饲高脂饲料。两组大鼠每日饮食量相同，自由饮水，每两周称体重一次，持续8周。根据造模组大鼠体重比对照组大鼠体重增加20％作为肥胖大鼠，共选出75只肥胖大鼠。
2．减肥实验：将75只造模成功的肥胖大鼠按体重和血清胆固醇随机分成五组，每组15只。第一组为空白对照组(normal control group，Con)，每只2ml／d蒸馏水灌胃；第二组为阳性对照组(Orlistat group，Orl)，给与奥利司他60mg·kg~(-1)·d~(-1)灌胃；其余三组为实验组：低剂量实验组(low-dosage experiment group of Jianzhi capsules，JZH1)，中剂量实验组(medium-dosage experiment group of Jianzhi capsules，JZH2)，高剂量实验组(high-dosage experiment group of Jianzhi capsules，JZH3)，分别以减之胶囊165mg·kg~(-1)·d~(-1)、250mg·kg~(-1)·d~(-1)、330mg·kg~(1-)·d~(-1)灌胃，减肥实验共40天，在减肥实验过程中，继续给予高脂饲料喂饲。其间每十天称体重一次；分别在减肥实验前，减肥实验第20天和第40天经尾部抽取各组大鼠血液，测定血清甘油三酯，胆固醇和高密度脂蛋白的浓度，减肥实验第40天同时测定空腹血清卵磷脂胆固醇酯酰转移酶(LCAT)，脂蛋白脂酶(LPL)，肝脂酶(HL)活性；实验结束前用乙醚麻醉，测量体长和尾长，计算Lees指数；在末次给药后24小时颈动脉放血致死，
浙江大学硕士学位论文
取腹部，肾周围及生殖器周围脂肪组织称重，其中生殖器周围脂肪组织经固定制片后，观察
脂肪细胞的大小和数目。
3.减之胶囊在体外对脂肪酶的抑制作用实验研究:
脂肪酶活性测定:取o.lml生理盐水，0、lml脂肪酶液[[Lipase]。v。，a.，=47.56Ulmll，加
37℃橄榄油乳剂3ml混合，立即置于紫外分光光度计340nm测定吸收值，连续记录
lomin，Zomin，30min，40min，50min，6omin的吸光度。同法测量o.lml减之胶囊(o.lm留ml)，o.lml
脂肪酶液，加橄榄油3ml时的酶活性。
不同剂量减之胶囊对脂肪酶活性影响:配制六个不同剂量的减之胶囊(m留ml):o、0.05、
0.1、0.15、0.2、0.25。测量不同剂量减之胶囊对脂肪酶活性的影响，方法如卜:将不同剂量
减之胶囊0.1 ml分别与0.lml脂肪酶液[[Lipase]。，r=47.56U/ml]，橄榄油乳剂3ml在37，C条夕I
下保温40分钟，在紫外分光光度计340nm处测定吸收值。
结果:
1.减之胶囊对肥胖大鼠体重的影响:减肥实验开始时，各组大鼠体重无明显区别，相
互比较无显著差异性(P>0.05).减肥实验第20天和第30天，实验组大鼠体重明显低于空
白对照组，相互比较有差异显著性(P<0.05)，与阳性对照组比较无显著性差异(P>0.05)。
实验第40天，低剂量实验组和中剂量实验组大鼠体重明显低于空白对照组，相互比较有差
异显著性(P<0.05)，与阳性对照组比较无显著性差异(P>0.05);高剂量实验组的大鼠体重
更明显低于空白对照组，两者比较有差异显著性(P<0.01);高剂量实验组的大鼠体重也明
显低于低、中剂量实验组，相互比较有差异显著性(P<0.05)，与阳性对照组比较也有差异
显著性(P<0.05)。
2.减之胶囊对肥胖大鼠内脏脂肪组织重量的影响:低、中、高剂量实验组的腹脂、肾
周脂、生殖器周围脂肪的重量均明显低于空白对照组，相互比较有差异显著性(P<0.05)，
与阳性对照组比较无显著差异性(P>0.05);实验组各组间无差异显著性(P>0.05)。
3.减之胶囊对肥胖大鼠】未。指数的影响:各实验组的Lees指数均明显低于空白对照
组.两者比较有显著差异性(P<0 .05)，与阳性对照组比较无显著差异性。其中高剂量实验
组与空白对照组的差异更大(P<0 .05)。实验组各组间无差异性(P>0.05)。
4.减之胶囊对肥胖大鼠脂肪组织形态的影响:实验各组的脂肪细胞直径均明显变小，
与空白对照组比较，有显著性差异(P劝.05)，与阳性对照组比较无差异显著性(P>0.05)，
不同剂量的减之胶囊对脂肪细胞大小影响不明显(P>0.05).各实验组脂肪细胞数目(每高
3
浙江大学硕士学位论文
倍视野)明显较空白对照组多，相互比较有显著性差异(P<0.05)，与阳性对照组比较无显
著性差异(P>0.05)，实验组组间比较无明显差异(P>0.05)。
5.减之胶囊对肥胖大鼠血清胆固醉(TC)、血清甘油三酣(TG)、血清高密度脂蛋白
(HDL)的影响:减肥实验开始时，各组大鼠血清胆固醇(TC)浓度无明显差异(P>0.05)。
实验结束时(第40天)减之胶囊实验组大鼠血清胆固醇(TC)明显低于空白对照组，相互
比较差别有显著性(P<0.05);减肥实验开始时，各组大鼠血清甘油三酷(TG)浓度无明显
差异(P>0.OS)，实验中期(20天)和实验结束时(40)，实验组和阳性对照组人鼠血清甘
油二酷(TG)浓度明显低于空白对照组，相互比较有显著性差异(P<0.05)。减肥实验开始
和结束时，各组大鼠血清高密度脂蛋白(HDL)的浓度无明显差异(
Objective: To assess the anti-obesity effect and mechanism of Jianzhi capsules on the obese rats induced by high fat diet.
Methods: Obesity was induced by high fat diet. Male SD strain rats (50g-70g),were randomly divided into control group(10 rats) and model group(80 rats),being given normal diet and high fat diet respectively for 8 weeks. Each rat had the same volume of diet per day and was weighed every two weeks.
After the models were prepared successfully, they were randomly divided into five groups:the normal control group (Con), the Orlistat control group (Orl), the low-dosage experiment group of Jianzhi capsules (JZHl),the medium-dosage experiment group of Jianzhi capsules (JZH2), the high-dosage experiment group of Jianzhi capsules (JZH3) (15 rats per group). The experiment groups were administered with Jianzhi capsules at the dosages of 165mg kg-1 d-1, 250mg kg-1d-1, 330mg kg-1d-1, while the Orlistat control group with Orlistat at the dosage of 60mg kg-1d-1. According to the dosage, the concentration of Jianzhi capsules and Orlistat of 2ml per day for one rat was prepared, while the normal control group with the same volume of distilled water. The experiment lasted 40 days. During the course ,the body weights were measured every ten days; The fast blood cholesterol (TC), triglycerides(TG),high-density lipoprotein (HDL),were tested at the beginning (the early stage), 20th day (the mid stage) and 40th day (the la
te stage); And at the late stage, the activity of the fast blood lecithin cholesterol acyltransferase (LCAT), lipoprotein lipase (LPL), hepatic lipase (HL) were also determined; By the end of the test, the rats were killed
and their fat tissues in the abdomenon,perirenal and perigenital were removed and weighed; After
the fat tissues in the perigenital were fixed and stained, the size and number of their fat cells were studies.
The study of inhibition of Jianzhi capsules on lipase in vitro: 0.lml Jianzhi capsules ,0.1 ml lipase and 3ml olive oil emulsion were mixed and measured immediately at the time of 10min,20min,30min,40min,50min,60min. A curve of product-time was obtained.Effect of six different dosages of Jianzhi capsules on the activity level of lipase were measured. The dosage of Jianzhi capsules (mg/ml) were: 0, 0.05, 0.1, 0.15, 0.2, 0.25. The method was the same as the above.At the time of 40 minutes, six different figures were obtained.
Results:
1. Effect of Jianzhi capsules on the body weight in the obese rats:
There were no differences in weights between the control groups and Jianzhi capsules groups before administration (p>0.05). After given Jianzhi capsules for 20 days and 30 days ,the weights in Jianzhi capsules groups were reduced significantly compared with the normal control group (P<0.05), however.there was no difference with the Orlistat control group.(P> 0.05). At the time of 40 days, the level of weights in the JZH1 and JZH2 was significantly decreased compared with the normal control group (P<0.05), however ,there was no difference with the Orlistat control group (P>0.05), and the level of weights in the JZH3 was obviously reduced compared with any other group (P<0.05).
2. Effect of Jianzhi capsules on the weights of fat tissues in the abdomnon, perirenal, perigenital in the obese rats:
The weights of fat tissues in Jianzhi groups were significantly reduced compared with those of the normal control group(P<0.05) while there was no difference with the Orlistat control group(P>0.05).
3. Effect of Jianzhi capsules on the Lees Indexes in the obese rats:
The Lees Indexes in the Jianzhi groups were significantly decreased compared with those of the normal control group(P<0.05), while there was no difference with the Orlistat control group(P>0.05).There were on significantly difference among the test groups.
4. Effect of Jianzhi capsules on the size and number of .fat cells in the perigenital:
The size of fat cells in the Jianhzi groups was significantly reduced compared with that in
normal control group (P<0.05) while there
引文
1．陈鹭颖。药物治疗肥胖病的治疗进展(J)。中国药学杂志，2002，37(11)：812-814。
2．吴平，胡永狮，杜青云，等。减肥药物的分类及其作用机制[J]。中国临床药学杂志，2000，9(2)：131-134。
3. Randle PJ, Garland PB, Hales CN, et al. The glucose-fatty acid cycle: its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 1: 785-789.
4. Davis C, Rifkind B, Brenner H, et al. A single cholesterol measurement underestimates the risk of CHD: an empirical example from the Lipid Research Clinics mortality follow-up study. JAMA 264: 3044-3046.
5．徐明彤，傅祖值。正确选用减肥药[J]。新医学，2002，33(11)：645-647。
6．何菊英，刘松青。决明子的药理作用及其临床应用[J]。药学实践杂志，2001，19(2)：111-113。
7．罗维，罗红，金胜基。菊花茶与决明茶保健作用[J]。时珍国医国药，1998，9(6)：579。
8．陆宗良，寇文熔，徐文枢，等。决明子散剂调节血脂的临床研究[J]。中国新药杂志，1998，7(6)：449。
9．裴蕾。芦荟的研究概况[J]。中医药信息，2001，18(4)：11-12。
10．谢光麟。芦荟中蒽醌衍生物的分离及其抗肿瘤活性的检测[J]。中国医科大学学报，1998，13(1)：571-573。
11．宁辉。中药降脂研究进展[J]。中国中药杂志．1999，24(3)：184。
12. Bartholome M, Niedmann D, Wieland H, et al. An optimized methods for measuring LCAT activity, independent of the concentration and quality of the physiological subtrate[J]. Biochim Biophys Acta, 1981, 664(2): 327-334.
13. Ray E V, Bellini F, Stoudt G, et al. Influence of lecithin: cholesterol acyltransferanse on cholesterol in hepatic cells and hepatocytes[J]. Biothin Biophys Acta, 1980, 617(2): 318-334.
14．蒋宪成，庄庆棋，梅美珍。卵磷脂胆固醇脂酰转移酶活性的简易测定法[J]。上海第一医学院学报，1985，12(2)：155-156。
15．叶水清。LCAT测定及其临床意义[J]。国外医学。临床生物化学与检验学分册，1986，(5)：6-10。
16. Krauss R M, Levy R I, Fredrickson D S. Selective measurement of two lipase activities in
postheparin plasma from normal subjects and patients with hyperlipoproteinemia[J]. J Clin Invest, 1974, 54(5): 1107-1124.
17. Blache D, Bouthillier D, Davignon J. Simple, reproducible procedure for selective measurement of lipoprotein lipase and hepatic lipase[J]. Clin Chem, 1983, 29(1): 154-158.
18．张蓉，刘宇，刘秉文。血浆脂蛋白及肝脂酶的比色测定法[J]。华西医科大学学报，1996，27(1)：106-110。
19．朱宁，郭军，陈润洁，等。反胶束体系中猪胰脂肪酶水解橄榄油的动力学研究[J]。药物研究，2002，11(8)。
20．杨振华，曾芝如。血清脂肪酶测定[M]。临床化学检验，441-444。
21. Zhang Y, Proenca R, Maffei M, et al. Positional cloning of the mouse obese gene and its human homologue[J]. Nature, 1994, 372: 425.
22. Thompson DB, Ravussin E, Bennett PH,e t al. Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians[J]. Hum Mol Genet, 1997, 6(5): 675.
23. Pelleymounter NA. Effects of the obese gene produce on body weght regulation in ob/ob mice[J]. Science, 1995, 269: 540-543.
24．蔡危威，曲伸，邹大进，等。高脂饮食肥胖大鼠制备的探讨[J]。现代康复，2000，4(12)：1854-1855。
25．孙志，张中成，刘志诚。营养性肥胖动物模型的实验研究[J]。中国药理学通报，2002，18(4)：466-467。
26. Ahrens EH, Hirsch J, Insull W, et al. The influence of dietary fats on serum-lipid levels in man[J]. Lancet 1: 943-953.
27. Keys A, Anderson JT, Grande F. Serum cholesterol response to changes in the diet. IV. Particular saturated fatty acids in the diet[J]. Metabolism 14: 776-787.
28. Hegsted DM, McGandy RB, Myers ML, et al. Quantitative effects of dietary fat on serum cholesterol in man[J]. Am J Clin Nutr 17: 281-295.
29. Dearden NM, Gibsonls, McDowallDG, et al. Effect of high-dose dexamethasone on outcome from severehead injury[J]. Neurosurg, 1986, 64: 81-88.
30．董爱森，朱惠，黄庆标，等。复方壳聚糖对高脂血症作用的研究[J]。福建医药杂志，2001．23(2)：104-106。
31．沈奇桂，朱寿民。决明子对实验性高胆固醇血症和动脉粥样硬化的抑制作用及其机理
探讨[J]。浙江医科大学学报，1993，22(6)：246-248。
32. Knittle JL, Timmers K, Ginsberg-Fellner F, et al. The growth of adipose tissue in children and adolescents: cross-sectional and longitudinal studies of adipose cell number and size[J]. J Clin Invest 63: 239-246.
33. Vague J. Degree of masculine differentiation of obesities: factor determining predisposition to diabetes, atherosclerosis, gout, and uric calculous disease[J]. Am J Clin Nutr 4: 20-34.
34. Yoshida T, Yoshika K, Sakane N, et al. Probocal presents the progression of fatty liver in MSG-treated obese mice[J]. Exp Clin Endocrinal. Diabetes, 1995, 103(2): 119-122.
35. Nestel PJ, Havel RJ, Bezman A. Sites of initial removal of chylomicron triglyceride fatty acids from the blood[J]. J Clin lnvest 41: 1915-1921.
36. Tikkanen M J, Nikkila E A. Regulation of hepatic lipase and serum lipoproteins by sex steroids[J]. Am Heart J, 1987, 113(2 Pt2): 562-567.
37．吴满平，黄如欣。大鼠肝内皮细胞脂酶代谢功能研究[J]。上海医科大学学报，1989，16(5)：351-355。
38. De Parscau L, Fielding P E. Lecithin cholesterol acyltransference cholesterol ester transfer activity from the isolated perfused rabbit liver[J]. J Lipid Res, 1984, 25(7): 721-728.
39．王淑如，朱力军。茶叶多糖对卵磷脂胆固醇酰基转移酶活性的影响[J]。中国药科大学学报，1993，24(2)：122-124。
40．Ekhard E, Ziegler L. J. Filer, JR(闻芝梅，陈君石：主译)。膳食脂肪[M]。现代营养学，第七版，人民卫生出版社，1998，4：44-56。
41. Shargo E, Spennetta T, Gordon E. Fatty acid synthesis in human adipose tissue[J]. J Biol Chem 244: 2761-2766.
42. Havel RJ, Kane JP. Structure and metabolism of plasma lipoproteins. In Scriver CR, Beaudet AL, Sly WS, Valle D(eds), The metabolic basis of inherited disease, 7~(th) ed. McGraw-Hill, New York, pp 1841-1851.
2．吴平，胡永狮，杜青云，等。减肥药物的分类及其作用机制[J]。中国临床药学杂志，2000，9(2)：131-134。
3. Randle PJ, Garland PB, Hales CN, et al. The glucose-fatty acid cycle: its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 1: 785-789.
4. Davis C, Rifkind B, Brenner H, et al. A single cholesterol measurement underestimates the risk of CHD: an empirical example from the Lipid Research Clinics mortality follow-up study. JAMA 264: 3044-3046.
5．徐明彤，傅祖值。正确选用减肥药[J]。新医学，2002，33(11)：645-647。
6．何菊英，刘松青。决明子的药理作用及其临床应用[J]。药学实践杂志，2001，19(2)：111-113。
7．罗维，罗红，金胜基。菊花茶与决明茶保健作用[J]。时珍国医国药，1998，9(6)：579。
8．陆宗良，寇文熔，徐文枢，等。决明子散剂调节血脂的临床研究[J]。中国新药杂志，1998，7(6)：449。
9．裴蕾。芦荟的研究概况[J]。中医药信息，2001，18(4)：11-12。
10．谢光麟。芦荟中蒽醌衍生物的分离及其抗肿瘤活性的检测[J]。中国医科大学学报，1998，13(1)：571-573。
11．宁辉。中药降脂研究进展[J]。中国中药杂志．1999，24(3)：184。
12. Bartholome M, Niedmann D, Wieland H, et al. An optimized methods for measuring LCAT activity, independent of the concentration and quality of the physiological subtrate[J]. Biochim Biophys Acta, 1981, 664(2): 327-334.
13. Ray E V, Bellini F, Stoudt G, et al. Influence of lecithin: cholesterol acyltransferanse on cholesterol in hepatic cells and hepatocytes[J]. Biothin Biophys Acta, 1980, 617(2): 318-334.
14．蒋宪成，庄庆棋，梅美珍。卵磷脂胆固醇脂酰转移酶活性的简易测定法[J]。上海第一医学院学报，1985，12(2)：155-156。
15．叶水清。LCAT测定及其临床意义[J]。国外医学。临床生物化学与检验学分册，1986，(5)：6-10。
16. Krauss R M, Levy R I, Fredrickson D S. Selective measurement of two lipase activities in
postheparin plasma from normal subjects and patients with hyperlipoproteinemia[J]. J Clin Invest, 1974, 54(5): 1107-1124.
17. Blache D, Bouthillier D, Davignon J. Simple, reproducible procedure for selective measurement of lipoprotein lipase and hepatic lipase[J]. Clin Chem, 1983, 29(1): 154-158.
18．张蓉，刘宇，刘秉文。血浆脂蛋白及肝脂酶的比色测定法[J]。华西医科大学学报，1996，27(1)：106-110。
19．朱宁，郭军，陈润洁，等。反胶束体系中猪胰脂肪酶水解橄榄油的动力学研究[J]。药物研究，2002，11(8)。
20．杨振华，曾芝如。血清脂肪酶测定[M]。临床化学检验，441-444。
21. Zhang Y, Proenca R, Maffei M, et al. Positional cloning of the mouse obese gene and its human homologue[J]. Nature, 1994, 372: 425.
22. Thompson DB, Ravussin E, Bennett PH,e t al. Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians[J]. Hum Mol Genet, 1997, 6(5): 675.
23. Pelleymounter NA. Effects of the obese gene produce on body weght regulation in ob/ob mice[J]. Science, 1995, 269: 540-543.
24．蔡危威，曲伸，邹大进，等。高脂饮食肥胖大鼠制备的探讨[J]。现代康复，2000，4(12)：1854-1855。
25．孙志，张中成，刘志诚。营养性肥胖动物模型的实验研究[J]。中国药理学通报，2002，18(4)：466-467。
26. Ahrens EH, Hirsch J, Insull W, et al. The influence of dietary fats on serum-lipid levels in man[J]. Lancet 1: 943-953.
27. Keys A, Anderson JT, Grande F. Serum cholesterol response to changes in the diet. IV. Particular saturated fatty acids in the diet[J]. Metabolism 14: 776-787.
28. Hegsted DM, McGandy RB, Myers ML, et al. Quantitative effects of dietary fat on serum cholesterol in man[J]. Am J Clin Nutr 17: 281-295.
29. Dearden NM, Gibsonls, McDowallDG, et al. Effect of high-dose dexamethasone on outcome from severehead injury[J]. Neurosurg, 1986, 64: 81-88.
30．董爱森，朱惠，黄庆标，等。复方壳聚糖对高脂血症作用的研究[J]。福建医药杂志，2001．23(2)：104-106。
31．沈奇桂，朱寿民。决明子对实验性高胆固醇血症和动脉粥样硬化的抑制作用及其机理
探讨[J]。浙江医科大学学报，1993，22(6)：246-248。
32. Knittle JL, Timmers K, Ginsberg-Fellner F, et al. The growth of adipose tissue in children and adolescents: cross-sectional and longitudinal studies of adipose cell number and size[J]. J Clin Invest 63: 239-246.
33. Vague J. Degree of masculine differentiation of obesities: factor determining predisposition to diabetes, atherosclerosis, gout, and uric calculous disease[J]. Am J Clin Nutr 4: 20-34.
34. Yoshida T, Yoshika K, Sakane N, et al. Probocal presents the progression of fatty liver in MSG-treated obese mice[J]. Exp Clin Endocrinal. Diabetes, 1995, 103(2): 119-122.
35. Nestel PJ, Havel RJ, Bezman A. Sites of initial removal of chylomicron triglyceride fatty acids from the blood[J]. J Clin lnvest 41: 1915-1921.
36. Tikkanen M J, Nikkila E A. Regulation of hepatic lipase and serum lipoproteins by sex steroids[J]. Am Heart J, 1987, 113(2 Pt2): 562-567.
37．吴满平，黄如欣。大鼠肝内皮细胞脂酶代谢功能研究[J]。上海医科大学学报，1989，16(5)：351-355。
38. De Parscau L, Fielding P E. Lecithin cholesterol acyltransference cholesterol ester transfer activity from the isolated perfused rabbit liver[J]. J Lipid Res, 1984, 25(7): 721-728.
39．王淑如，朱力军。茶叶多糖对卵磷脂胆固醇酰基转移酶活性的影响[J]。中国药科大学学报，1993，24(2)：122-124。
40．Ekhard E, Ziegler L. J. Filer, JR(闻芝梅，陈君石：主译)。膳食脂肪[M]。现代营养学，第七版，人民卫生出版社，1998，4：44-56。
41. Shargo E, Spennetta T, Gordon E. Fatty acid synthesis in human adipose tissue[J]. J Biol Chem 244: 2761-2766.
42. Havel RJ, Kane JP. Structure and metabolism of plasma lipoproteins. In Scriver CR, Beaudet AL, Sly WS, Valle D(eds), The metabolic basis of inherited disease, 7~(th) ed. McGraw-Hill, New York, pp 1841-1851.
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