黄芪多糖对人工感染法氏囊病毒雏鸡红细胞免疫功能的影响研究
摘要
临床健康海兰白雏鸡320羽，随机分为8组，每组40羽，1组为对照组，2～8组为试验组。为确定红细胞免疫粘附活性测定影响因素，于21日龄心脏采血，分别测定红细胞C_(3b)受体花环率(E-C_(3b)RR)、红细胞免疫复合物花环率(E-ICRR)、红细胞C_(3b)受体花环促进率(ERER)和红细胞C_(3b)受体花环抑制率(ERIR)，结果各指标在组间差异不显著(P＞0.05)。表明测定方法符合试验要求。
8组鸡21日龄时经传染性法氏囊病毒(IBDV)抗体测定均为阴性。26日龄时用IBDV0.3ml／羽对2～8组试验鸡攻毒。1、2组不使用(APS)，3～8组经胸肌注射APS，其中3、4组于攻毒前3d连续注射，5、6组从攻毒当日起连续注射6d，7、8组从攻毒当日连续注射3d，APS剂量：3、5、7组为5mg／羽，4、6、8组为10mg／羽。各组鸡分别在29、32、35、38日龄时心脏采血，对E-C_(3b)RR、E-ICRR、ERER、ERIR及红细胞中SOD活性和MDA含量进行测定。结果如下：
1．雏鸡感染IBDV后，与对照组相比，试验2组E-C_(3b)RR、ERER及红细胞中SOD活性均降低，且差异显著(P＜0.05)，在38日龄时SOD值与对照组仍差异极显著(P＜0.01)；试验2组E-ICRR、ERIR及红细胞中MDA含量均增高，但E-ICRR仅在29日龄时差异显著(P＜0.05)，ERIR在各日龄时均差异不显著(P＞0.05)，红细胞中MDA含量在32、35、38日龄时差异显著(P＜0.05)。表明感染IBDV雏鸡其红细胞免疫粘附能力、清除免疫复合物能力、抗氧化能力及血清中红细胞免疫促进因子活性均降低，呈现红细胞免疫功能低下。
2．APS各处理组与试验2组相比，E-C_(3b)RR、E-ICRR、ERER及红细胞中SOD活性均有不同程度升高，其中，E-C_(3b)RR在5、6组除38日龄外，4组在29日龄时，7、8组在29、32日龄时均与2组存在显著差异(P＜0.05)；E-ICRR在6组除38日龄外，其余各APS处理组在29日龄时与试验2组差异显著(P＜0.05)；ERER在7组29日龄时，6组35日龄时均与2组差异显著(P＜0.05)；
红细胞中SOD活性，在6、8组29日龄时，5一8组32日龄时，各APS处理组
35、38日龄时与2组均差异极显著(P<0.01)。
各APS处理组，ERIR及红细胞中MDA含量与2组相比有不同程度降低，
其中ERIR在6组仅32日龄时与2组差异不显著(P>0.05)，7组29日龄、
5组38日龄时与2组均差异显著(P<0.05);红细胞中MDA含量在3组38日
龄时，4一8组32、35、38日龄时与2组均差异显著(P<0.05)。表明APS可提
高感染工BDv雏鸡红细胞免疫粘附能力、清除免疫复合物能力和杭氧化能力，
可降低血清中红细胞免疫粘附抑制因子活性，进而提高红细胞免疫功能。
3.经APS处理的各组间相比，6组E一C3bRR、E一ICRR、ERER及红细胞中SOD
活性均高于其它各组，其中6组E一饥RR在29日龄时与3一5组、32日龄时与
各APS处理组及35日龄时与3、4、7和8组存在显著差异(P<0.肠);6组
E一ICRR在29日龄时与3、4、5、8组、35日龄时与3、7、8组存在显著差异
(P<0.肠);6组ERER与各/\PS组均差异不显著(P>0.肠);6组红细胞中
SOD活性在32日龄与5，7和8组、35日龄与5和7组差异不显著(P>0.肠)
外，其余各次测定值差异极显著(P<0.沮);6组ER皿及红细胞MDA含量均
低于其它各组，但ERIR与其它各组均差异不显著(P>0.05);6组红细胞中
MDA含量仅在35日龄时与3组存在显著差异(P<0.05)。表明经APS处理的
各组中以6组对红细胞免疫增强作用最显著。
320 Haline White Chickens, being clinical healthy, were randomly divided into 8 groups, 40 for each group. Group 1 was the control and Group 2 to 8, the experimental groups. Blood samples were taken from heart at 21-day-age and the E-C3bRR, E-ICRR, ERER and ERIR were determined respectively. The values had no statistic difference among 8 groups(P>0.05). The results showed that the determing methods were stable and could be used in the experiment.
IBDV antibodies in serum samples of all chickens were negative at 21-day-age. Groups 2 to 8 were infected with IBDV (0.3ml each) at 26-day-age. Group 1 and 2 were not treated with APS, and groups 3 to 8 were injected with APS in chest muscle. Group 3 and 4 were injected at 23 to 25days of age, group 5 and 6, at 26 to 31 days of age, and group 7 and 8, at 26 to 28 days of age. The dosage of APS was 5mg each in groups 3,5 and 7, and lOmg each in groups 4,6 and 8. Blood samples were taken from heart when the chickens were 29,32,35 and 38 days of age respectively, and the E-C3bRR, E-ICRR, ERER, ERIR and the activity of SOD and the contents of MDA in erythrocyte were determined. The results were as follows:
l.The E-C3bRR, ERER and the activity of SOD in erythrocyte of Chickens in group 2 were significantly lower than the controls (P<0.05), and the E-ICRR, ERIR and the content of MDA in erythrocyte were significantly higher than the control (P<0.05). E-ICRR between group 1 and 2 were statistically different only at 29-day-age (PO.05). ERIR were not statistically different at all ages (P>0.05). The contents of MDA in erythrocyte were obviously different at 32,35,38-day-age (P<0.05). The results showed that the immune functions of erythrocyte were decreased when chickens were infected with IBDV.
2.Compared with group 2, the E-C3bRR, E-ICRR, ERER and the activity of SOD in erythrocyte of groups injected with APS were increased. E-C3bRR, except for at 38-day-age in group 5 and 6, at 29-day-age in group 4, at 29 and 32-day-age in group 7 and 8 were significantly different from group 2 (PO.05). ERER at 29-day-age in group 8 and at 35-day-age in group 6 were obviously different from group2 (PO.05). The activity of SOD in erythrocyte at 29-day-age in group 6 and 8, at 32-day-age in group 5 to 8, at 35,38-day-age of
all groups injected APS were mostly different from group 2 (PO.05).
Compared with group 2, ERIR and the content Of MDA in erythrocyte of groups injected with APS were decreased. ERIR, except for at 32-day-age in group 6, at 29-day-age in group 7, at 38-day-age in group 5 were significantly different from group 2 (PO.05). The content of MDA in erythrocyte at 38-day-age in group 3 and at 32,35,38-day-age in group 4 to 8 were obviously different from group 2 (PO.05). The results indicated that APS could improve the immune function of erythrocyte.
3.The E-C3bRR, E-ICRR, ERER and activity of SOD in erythrocyte of group 6 were the highest among groups injected with APS. E-C3bRR at 29-day-age in groups 3 to 5, at 32-day-age in all groups treated with APS and at 35-day-age in group 3,4 and 7 were significantly different from group 6(P<0.05). ERIR at 29-day-age in groups 3 to 5 and 8, at 35-day-age in groups3, 7 and 8 were significantly different from group 6 (PO.05). ERER had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The activity of SOD in erythrocyte, except for at 32-day-age in groups 5,7 and 8 and 35-day-age in group 5 and 7, the others were significantly different from group 6 (P<0.01). ERIR and the content of MDA in erythrocyte of group 6, were the lowest in all groups injected with APS. And ERIR had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The content of MDA in erythrocyte had obvious difference between group 6 and group 3 at 35-day-age (PO.05). The results
indicated that grup 6 had the most significant effect on the immune function of erythrocyte in chickens among the groups injected with APS.
8组鸡21日龄时经传染性法氏囊病毒(IBDV)抗体测定均为阴性。26日龄时用IBDV0.3ml／羽对2～8组试验鸡攻毒。1、2组不使用(APS)，3～8组经胸肌注射APS，其中3、4组于攻毒前3d连续注射，5、6组从攻毒当日起连续注射6d，7、8组从攻毒当日连续注射3d，APS剂量：3、5、7组为5mg／羽，4、6、8组为10mg／羽。各组鸡分别在29、32、35、38日龄时心脏采血，对E-C_(3b)RR、E-ICRR、ERER、ERIR及红细胞中SOD活性和MDA含量进行测定。结果如下：
1．雏鸡感染IBDV后，与对照组相比，试验2组E-C_(3b)RR、ERER及红细胞中SOD活性均降低，且差异显著(P＜0.05)，在38日龄时SOD值与对照组仍差异极显著(P＜0.01)；试验2组E-ICRR、ERIR及红细胞中MDA含量均增高，但E-ICRR仅在29日龄时差异显著(P＜0.05)，ERIR在各日龄时均差异不显著(P＞0.05)，红细胞中MDA含量在32、35、38日龄时差异显著(P＜0.05)。表明感染IBDV雏鸡其红细胞免疫粘附能力、清除免疫复合物能力、抗氧化能力及血清中红细胞免疫促进因子活性均降低，呈现红细胞免疫功能低下。
2．APS各处理组与试验2组相比，E-C_(3b)RR、E-ICRR、ERER及红细胞中SOD活性均有不同程度升高，其中，E-C_(3b)RR在5、6组除38日龄外，4组在29日龄时，7、8组在29、32日龄时均与2组存在显著差异(P＜0.05)；E-ICRR在6组除38日龄外，其余各APS处理组在29日龄时与试验2组差异显著(P＜0.05)；ERER在7组29日龄时，6组35日龄时均与2组差异显著(P＜0.05)；
红细胞中SOD活性，在6、8组29日龄时，5一8组32日龄时，各APS处理组
35、38日龄时与2组均差异极显著(P<0.01)。
各APS处理组，ERIR及红细胞中MDA含量与2组相比有不同程度降低，
其中ERIR在6组仅32日龄时与2组差异不显著(P>0.05)，7组29日龄、
5组38日龄时与2组均差异显著(P<0.05);红细胞中MDA含量在3组38日
龄时，4一8组32、35、38日龄时与2组均差异显著(P<0.05)。表明APS可提
高感染工BDv雏鸡红细胞免疫粘附能力、清除免疫复合物能力和杭氧化能力，
可降低血清中红细胞免疫粘附抑制因子活性，进而提高红细胞免疫功能。
3.经APS处理的各组间相比，6组E一C3bRR、E一ICRR、ERER及红细胞中SOD
活性均高于其它各组，其中6组E一饥RR在29日龄时与3一5组、32日龄时与
各APS处理组及35日龄时与3、4、7和8组存在显著差异(P<0.肠);6组
E一ICRR在29日龄时与3、4、5、8组、35日龄时与3、7、8组存在显著差异
(P<0.肠);6组ERER与各/\PS组均差异不显著(P>0.肠);6组红细胞中
SOD活性在32日龄与5，7和8组、35日龄与5和7组差异不显著(P>0.肠)
外，其余各次测定值差异极显著(P<0.沮);6组ER皿及红细胞MDA含量均
低于其它各组，但ERIR与其它各组均差异不显著(P>0.05);6组红细胞中
MDA含量仅在35日龄时与3组存在显著差异(P<0.05)。表明经APS处理的
各组中以6组对红细胞免疫增强作用最显著。
320 Haline White Chickens, being clinical healthy, were randomly divided into 8 groups, 40 for each group. Group 1 was the control and Group 2 to 8, the experimental groups. Blood samples were taken from heart at 21-day-age and the E-C3bRR, E-ICRR, ERER and ERIR were determined respectively. The values had no statistic difference among 8 groups(P>0.05). The results showed that the determing methods were stable and could be used in the experiment.
IBDV antibodies in serum samples of all chickens were negative at 21-day-age. Groups 2 to 8 were infected with IBDV (0.3ml each) at 26-day-age. Group 1 and 2 were not treated with APS, and groups 3 to 8 were injected with APS in chest muscle. Group 3 and 4 were injected at 23 to 25days of age, group 5 and 6, at 26 to 31 days of age, and group 7 and 8, at 26 to 28 days of age. The dosage of APS was 5mg each in groups 3,5 and 7, and lOmg each in groups 4,6 and 8. Blood samples were taken from heart when the chickens were 29,32,35 and 38 days of age respectively, and the E-C3bRR, E-ICRR, ERER, ERIR and the activity of SOD and the contents of MDA in erythrocyte were determined. The results were as follows:
l.The E-C3bRR, ERER and the activity of SOD in erythrocyte of Chickens in group 2 were significantly lower than the controls (P<0.05), and the E-ICRR, ERIR and the content of MDA in erythrocyte were significantly higher than the control (P<0.05). E-ICRR between group 1 and 2 were statistically different only at 29-day-age (PO.05). ERIR were not statistically different at all ages (P>0.05). The contents of MDA in erythrocyte were obviously different at 32,35,38-day-age (P<0.05). The results showed that the immune functions of erythrocyte were decreased when chickens were infected with IBDV.
2.Compared with group 2, the E-C3bRR, E-ICRR, ERER and the activity of SOD in erythrocyte of groups injected with APS were increased. E-C3bRR, except for at 38-day-age in group 5 and 6, at 29-day-age in group 4, at 29 and 32-day-age in group 7 and 8 were significantly different from group 2 (PO.05). ERER at 29-day-age in group 8 and at 35-day-age in group 6 were obviously different from group2 (PO.05). The activity of SOD in erythrocyte at 29-day-age in group 6 and 8, at 32-day-age in group 5 to 8, at 35,38-day-age of
all groups injected APS were mostly different from group 2 (PO.05).
Compared with group 2, ERIR and the content Of MDA in erythrocyte of groups injected with APS were decreased. ERIR, except for at 32-day-age in group 6, at 29-day-age in group 7, at 38-day-age in group 5 were significantly different from group 2 (PO.05). The content of MDA in erythrocyte at 38-day-age in group 3 and at 32,35,38-day-age in group 4 to 8 were obviously different from group 2 (PO.05). The results indicated that APS could improve the immune function of erythrocyte.
3.The E-C3bRR, E-ICRR, ERER and activity of SOD in erythrocyte of group 6 were the highest among groups injected with APS. E-C3bRR at 29-day-age in groups 3 to 5, at 32-day-age in all groups treated with APS and at 35-day-age in group 3,4 and 7 were significantly different from group 6(P<0.05). ERIR at 29-day-age in groups 3 to 5 and 8, at 35-day-age in groups3, 7 and 8 were significantly different from group 6 (PO.05). ERER had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The activity of SOD in erythrocyte, except for at 32-day-age in groups 5,7 and 8 and 35-day-age in group 5 and 7, the others were significantly different from group 6 (P<0.01). ERIR and the content of MDA in erythrocyte of group 6, were the lowest in all groups injected with APS. And ERIR had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The content of MDA in erythrocyte had obvious difference between group 6 and group 3 at 35-day-age (PO.05). The results
indicated that grup 6 had the most significant effect on the immune function of erythrocyte in chickens among the groups injected with APS.
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46. VasconcelosA.C. LamK.M.Apoptosis induced by infectious bursal disease virus [J].Journal of Genera 1 Virology. 1994,(75): 1803～1806
47．郭峰等．单克隆抗体在红细胞免疫粘附功能研究方面的应用[J]．中华血液学杂志，1985，6(6)：333
48．郭峰．酵母多糖血凝法测定红细胞免疫粘附功能实验的建立及应用[J]．中国免疫学杂志，1991，7(增刊)：36
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51. Mulligan MS,Yeh CG,Rudolph AR, et al. Protective effects of soluble CRlin complement and nertrophil-mediated tissue injury [J]. Immunology, 1992,148(5): 1479
52. Forslid j, Hed J, Stendahl O. Erythrocyte enhancement of C_(3b)-mediated phagocytosis in human neutrophils in vitro:A combined effect of the erythrocyte complement receptor CR1 and erythrocyte scavengers to reactive oxyen metabolites[J].Immunology, 1985,55(1): 97
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54. Shau H. Golub SH. Modulation of natural killer mediated lysis by red blood cells [J]. Cell Immunol, 1988, 116(1): 1
55．王金胜主编．农业生物化学技术[M]．山西科学技术出版社，1997，144～146
56．中山大学生物系生化微生物学研究室编．生化技术导论[M]．人民教育出版社，1987，9
57．刘聚祥，胡维华，刘建明，等．鸡包涵体肝炎病毒(IBHV)对雏鸡淋巴细胞和红细胞免疫功能的影响[J]．中国兽医学报，1999，(19)4：330～332
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62．郭峰．三种常用红细胞免疫试剂的制备和使用[J]．第二军医大学学报，1995，4：18～20
63．黄盛东，郭峰．红细胞免疫调控机理研究新进展[J]．国外医学免疫学分册 1991，(14)4：191～195
64．郭峰，刘小平，徐瑛．天花粉对小鼠艾氏腹水癌的疗效和对红细胞免疫功能的影响[J]．中西医结合杂志，1989，(9)7：418～420
65．郭峰，张延东．血清对红细胞免疫粘附抑制作用的测定[J]．上海免疫学杂志．1987，(7)3：133～135
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