围产期奶牛血清中GH、IGF-Ⅰ及部分生化指标变化规律的研究
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摘要
本实验以处于围产期的57头奶牛为研究对象,通过实验室检测结合临床症状查出其中健康的经产牛、健康的初产牛及患酮病、脂肪肝、胎衣不下的自然病例,于奶牛产前15d,产前7d,产前1d,产后1d,产后7d,产后15d和产后22d分别采颈静脉血,检测血清中生长激素、胰岛素生长因子Ι、白蛋白、总蛋白、尿素氮、丙二醛及谷胱甘肽过氧化物酶、超氧化物岐化酶的含量及活力,根据其动态变化作了统计分析。
实验结果显示:
1.各组奶牛血清中GH、IGF-I基本上呈波动变化,但总体上患酮病、胎衣不下、脂肪肝奶牛在产后7d血清中GH含量不同程度地高于对照组奶牛,IGF-I含量不同程度地低于对照组奶牛。
2.总体上,患病奶牛血清中白蛋白含量变化基本上和对照组奶牛一致,总蛋白含量不同程度地低于对照组奶牛,尿素氮含量不同程度地高于(患胎衣不下奶牛除外)对照组奶牛。
3.患病奶牛血清中GSH-Px、T-SOD活力不同程度低于对照组奶牛,而MDA含量不同程度地高于对照组奶牛。揭示了患病奶牛的抗氧化能力低于健康奶牛。
4.对实验结果分析得出:所测得围产期奶牛血清中GH、IGF-I、白蛋白、总蛋白、尿素氮、GSH-Px、T-SOD、MDA的动态变化与围产期奶牛能量负平衡,体脂动员、蛋白质代谢紊乱密切相关。
57 cows in the perinatal period were used in the experiment. Through clinical symptom and laboratory detection, they were divided into five groups , healthy multiparity cows, healthy primiparity cows, cows with fatty liver disease, cows with ketosis and cows with retention fetal membrance. We tested the blood of the 15, 7, 1 days in antepartum and 1, 7, 15, 22 days postnatally, and determined the levels of the growth hormone, lnsulin-like growth factorⅠ, albumin, total protein, urea nitrogen ,malondialdehyde and glutathione peroxidase , hepatocuprein in blood-serum. The data were analyzed statistically.
The results showed as followings:
1. The levels of the GH and IGF-I in blood serum in each group presented fluctuating were changed essentially during the Perinatal Period. But the levels of GH in the serum of the cows with the diseases were higher than those of the control group in post partum 7 days in the different levels, and the content of IGF-I were lower than those of the control group cows in the different levels.
2. Generally, the content changes in the serum ALB of the cows with diseases were most likely with that of the control group, the content of TP were lower than the control group in the different levels. (except for the cows with the retention of fetal membranes).
3. The activity of the serum GSH-Px and T-SOD in cows with diseases were lower than those of the control group in the different levels, and the content of MDA were higher than those of the control group in the different levels. It revealed that the serum capability of antioxidation in the cows with disease was lower than those of healthy cows.
4. The analysis of the experimental results showed that: the content changes of GH, IGF-I, ALB, TP, BUN, GSH-Px, T-SOD, MDA in cows’serum presented fluctuating changes in the perinatal period. These relationships were closed with negatively balance in energy, aroused body fat and protein metabolic disturbance.
实验结果显示:
1.各组奶牛血清中GH、IGF-I基本上呈波动变化,但总体上患酮病、胎衣不下、脂肪肝奶牛在产后7d血清中GH含量不同程度地高于对照组奶牛,IGF-I含量不同程度地低于对照组奶牛。
2.总体上,患病奶牛血清中白蛋白含量变化基本上和对照组奶牛一致,总蛋白含量不同程度地低于对照组奶牛,尿素氮含量不同程度地高于(患胎衣不下奶牛除外)对照组奶牛。
3.患病奶牛血清中GSH-Px、T-SOD活力不同程度低于对照组奶牛,而MDA含量不同程度地高于对照组奶牛。揭示了患病奶牛的抗氧化能力低于健康奶牛。
4.对实验结果分析得出:所测得围产期奶牛血清中GH、IGF-I、白蛋白、总蛋白、尿素氮、GSH-Px、T-SOD、MDA的动态变化与围产期奶牛能量负平衡,体脂动员、蛋白质代谢紊乱密切相关。
57 cows in the perinatal period were used in the experiment. Through clinical symptom and laboratory detection, they were divided into five groups , healthy multiparity cows, healthy primiparity cows, cows with fatty liver disease, cows with ketosis and cows with retention fetal membrance. We tested the blood of the 15, 7, 1 days in antepartum and 1, 7, 15, 22 days postnatally, and determined the levels of the growth hormone, lnsulin-like growth factorⅠ, albumin, total protein, urea nitrogen ,malondialdehyde and glutathione peroxidase , hepatocuprein in blood-serum. The data were analyzed statistically.
The results showed as followings:
1. The levels of the GH and IGF-I in blood serum in each group presented fluctuating were changed essentially during the Perinatal Period. But the levels of GH in the serum of the cows with the diseases were higher than those of the control group in post partum 7 days in the different levels, and the content of IGF-I were lower than those of the control group cows in the different levels.
2. Generally, the content changes in the serum ALB of the cows with diseases were most likely with that of the control group, the content of TP were lower than the control group in the different levels. (except for the cows with the retention of fetal membranes).
3. The activity of the serum GSH-Px and T-SOD in cows with diseases were lower than those of the control group in the different levels, and the content of MDA were higher than those of the control group in the different levels. It revealed that the serum capability of antioxidation in the cows with disease was lower than those of healthy cows.
4. The analysis of the experimental results showed that: the content changes of GH, IGF-I, ALB, TP, BUN, GSH-Px, T-SOD, MDA in cows’serum presented fluctuating changes in the perinatal period. These relationships were closed with negatively balance in energy, aroused body fat and protein metabolic disturbance.
引文
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