抗痛风中药的体外活性筛选

详细信息    查看官网全文

• 英文论文题名：Screening the anti-gout traditional herbs from TCM using an in vitro method
• 论文作者：陈维佳 ; 武毅 ; 赵芯 ; 刘舒 ; 宋凤瑞 ; 刘忠英 ; 刘志强
• 英文论文作者：Wei-Jia Chen ; Yi Wu ; Xin Zhao ; Shu Liu ; Feng-Rui Song ; Zhong-Ying Liu ; Zhi-Qiang Liu ; School of Pharmaceutical Sciences ; Jilin University ; Changchun Institute of Applied Chemistry ; Chinese Academy of Sciences
• 年：2016
• 作者机构：吉林大学药学院;中国科学院长春应用化学研究所;
• 论文关键词：痛风 ; 炎症 ; 黄嘌呤氧化酶 ; 人脐静脉血管内皮细胞 ; 高效液相色谱-质谱/质谱
• 英文论文关键词：gout ; inflammation ; XOD ; HUVEC ; UPLC-MS/MS
• 会议召开时间：2016-07-01
• 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
• 语种：中文
• 分类号：R285
• 学会代码：ZGHY
• 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
• 会议地点：中国辽宁大连
• 主办单位：中国化学会
• 学会名称：中国化学会
• 页数：1
• 文件大小：212k
• 原文格式：D
• 会议级别：全国

摘要

目的:评价33味中药提取物的抗炎作用和抑制黄嘌呤氧化酶(XOD)的活性。方法:通过尿酸钠(MSU)刺激人脐静脉血管内皮细胞(HUVEC)建立体外急性痛风模型,并测定细胞上清液和细胞裂解液中ICAM-1和IL-1β的表达水平来评价体外痛风模型的可行性;通过体外细胞模型筛选中药提取物及标准品对MSU刺激的体外急性痛风模型的抗炎作用:通过超高效液相色谱-质谱/质谱(UPLC-MS/MS)的方法监测黄嘌呤氧化生成尿酸的含量,反应中药提取物及标准品对XOD的抑制作用。结果:丹参、大黄、虎杖、卷柏、赤芍,尤其是银杏叶能够更好的抑制MSU诱导的炎症和抑制XOD活性,这与其含有的主要活性成分黄酮类和蒽醌类相关。
The aim of this work was to evaluate the ability of 33 herbal extracts in inhibiting the acute inflammation and XOD activity.The anti-inflammation effects of the herbal extracts were detected by an in vitro cell model,which was established by stimulating HUVEC using MSU.In this model,the ICAM-1 and IL-1β were expressed,and the anti-inflammation effects of herbal extracts were evaluated by detecting the content changes of ICAM-1 and IL-1β in cell lysates and cell culture supemates using ELISA.Moreover,the UPLC-MS/MS method was used for the screening of XOD inhibitors in this research.The amount of uric acid from each analyte was directly detected using the multiple reaction monitoring mode.Results indicated that the anti-inflammation and enzyme inhibitory activities of the herbal extracts may be correlated with their bioactive components.The compounds of flavonoid and anthraquinone seem to be more effective on inflammation and inhibiting XOD.

引文

[1]Z Miao Z,Li C,Chen Y,Zhao S,et al.Dietary and lifestyle changes associated with high prevalence of hyperuricemia and gout in the Shandong coastal cities of Eastern China.J Rheumatol.2008,35(9):1859-64.
    [2]Kuo CF,Grainge MJ,Zhang W,Doherty M.Global epidemiology of gout:prevalence,incidence and risk factors.Nat Rev Rheumatol.2015 Nov;11(11):649-62.