基于碳、氮稳定同位素技术的大亚湾紫海胆食性分析
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- 英文论文题名：Feeding habits of the purple sea urchin Anthocidaris crassispina based on stable carbon and nitrogen isotope analysis
- 论文作者：莫宝霖 ; 秦传新 ; 陈丕茂 ; 袁华荣 ; 黎小国 ; FENG Xue ; TONG Fei
- 英文论文作者：MO Bao-lin ; QIN Chuan-xin ; CHEN Pi-mao ; YUAN Hua-rong ; FENG Xue ; TONG Fei ; South China Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; Key Laboratory of Marine Ranching Technology ; Chinese Academy of Fishery Sciences ; Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment ; Ministry of Agriculture ; Tianjin Agricultural University
- 年：2017
- 作者机构：中国水产科学研究院南海水产研究所;天津农学院;农业部南海渔业资源环境科学观测实验站;中国水产科学研究院海洋牧场技术重点实验室;
- 论文关键词：紫海胆 ; 碳稳定同位素 ; 氮稳定同位素 ; 食性 ; 大亚湾
- 英文论文关键词：Anthocidaris crassispina ; stable carbon and nitrogen isotopes ; feeding habits ; Daya Bay
- 会议召开时间：2017-03-31
- 会议录名称：第一届微藻与水族高峰论坛论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：CHJT
- 会议名称：第一届微藻与水族高峰论坛
- 会议地点：中国天津
- 主办单位：中国水产学会观赏鱼分会
- 学会名称：天津现代晨辉科技集团有限公司
- 页数：11
- 文件大小：1612k
- 原文格式：D
- 会议级别：全国
摘要
为掌握大亚湾紫海胆(Anthocidaris crassispina)的食性特征,应用碳、氮稳定同位素技术对2015年8月所采集紫海胆样本的稳定同位素特征、营养级和食性特征进行了初步研究。结果表明,大亚湾紫海胆平均δ~(13)C值为-(13.35±1.21)‰,平均δ~(15)N值为(9.14±0.38)‰,平均营养级为2.11±0.14。不同壳径紫海胆之间的碳、稳定同位素比值无显著性差异(P0.05)。大亚湾海域紫海胆生活环境周围生物δ~(13)C值分布范围为最小值-(20.76±1.42)‰~最大值-(9.93±1.59)‰,δ~(15)N值分布范围为最小值-0.16±1.34~最大值14.99±0.00‰,营养级范围为最小值1.34~最大值3.77。大亚湾主要生物种类可划分为悬浮物、初级生产者和初级消费者、次级消费者、顶级消费者4个营养组群,其中紫海胆属于次级消费者。8月份调查海域珊瑚稀少,大型海藻密度低且死亡降解形成颗粒有机物(Particulate Organic Matter,POM),陆源POM随降雨大量流入大亚湾,导致紫海胆在8月份摄食偏向碎屑食物链,主要食物来源为POM,平均贡献率为67.3%;其余摄食种类为沉积物(Sediment Organic Matter,SOM)、裂叶马尾藻(Scagassum siliquastrum)、底栖硅藻、浮游动物及浮游植物,平均贡献率分别为9.7%、9.3%、6.7%、3.7%及3.3%。大亚湾紫海胆摄食种类与其栖息地底栖生物存在重叠,具有一定的食物竞争关系。研究表明,分析紫海胆食性特征对了解其所在生态系统中营养级水平具有重要意义。
Purple sea urchin Anthocidaris crassispina is an important benthic species in the western Pacific Ocean. In recent years, environmental disruption and over-exploitation have damaged the resources of this species. Stable isotope analysis has become a powerful tool for studying the feeding habits of marine animals, and this method can overcome some of the limitations associated with traditional stomach contents analysis. Carbon(δ~(13)C) and nitrogen(δ~(15)N) were used as tools to evaluate the stable isotopic characteristics, trophic levels, shell diameter, and feeding habits of A. crassispina, from Daya Bay on the south coast of Guangdong Province, using data collected in August 2015. In the present study, the trophic levels of A. crassispina and other benthic species in Daya Bay were based on δ~(15)N. Average δ~(13)C of A. crassispina was-13.35 ± 1.21‰, average δ~(15)N was 9.14 ± 0.38‰, and the trophic levels ranged from 2.09 to 2.13(mean 2.11); no significant correlation was found between the different shell diameters of the A. crassispina sampled and the recorded values of δ~(13)C and δ~(15)N(P>0.05). Average δ~(13)C values of thepotential food sources were between -9.93 ± 1.59‰ and -20.76 ± 1.42‰, and their average δ~(15)N values were between -0.16 ± 1.34‰ and 14.99‰; the trophic levels ranged from 1.34 to 3.77. Cluster analysis showed that the food web among major species of Daya Bay could be classified into four trophic groups: suspended organic matter, primary producers and primary consumers, secondary consumers, and top predators. There were two main channels in the overall food chain: one was the grazing food chain, and the other was the detritus food chain. A. crassispina have a tendency to feed on detritus in August, when corals are scarce, macroalgae density is low, and the terrigenous materials present as particulate organic matter(POM) into Daya Bay follow a period of heavy rains. The main food resources of A. crassispina were: POM, sediment organic matter(SOM), the brown macroalgae Sargassum siliquastrum, bacillariophyceae(diatoms), zooplankton and phytoplankton Among these, POM was the most important food item for A. crassispina, accounting for 67.3% of its food consumption; the other food categories accounted for 9.7%, 9.3%, 6.7%, 3.7% and 3.3% of its food composition, respectively. Considerable competition exists between A. crassispina and the benthos, which suggests urgent efforts are needed to improve this ecological environment as well as to increase the seaweed biomass. Variations in the trophic levels were related to differences in the isotope baseline, and temporal and spatial variations. There was no significant correlation between shell diameter of A. crassispina and its feeding habits, perhaps because its food source is relatively singular. Thus, it is evident that feeding habits play an important role in population resource recovery and ecological niches of the system.
Purple sea urchin Anthocidaris crassispina is an important benthic species in the western Pacific Ocean. In recent years, environmental disruption and over-exploitation have damaged the resources of this species. Stable isotope analysis has become a powerful tool for studying the feeding habits of marine animals, and this method can overcome some of the limitations associated with traditional stomach contents analysis. Carbon(δ~(13)C) and nitrogen(δ~(15)N) were used as tools to evaluate the stable isotopic characteristics, trophic levels, shell diameter, and feeding habits of A. crassispina, from Daya Bay on the south coast of Guangdong Province, using data collected in August 2015. In the present study, the trophic levels of A. crassispina and other benthic species in Daya Bay were based on δ~(15)N. Average δ~(13)C of A. crassispina was-13.35 ± 1.21‰, average δ~(15)N was 9.14 ± 0.38‰, and the trophic levels ranged from 2.09 to 2.13(mean 2.11); no significant correlation was found between the different shell diameters of the A. crassispina sampled and the recorded values of δ~(13)C and δ~(15)N(P>0.05). Average δ~(13)C values of thepotential food sources were between -9.93 ± 1.59‰ and -20.76 ± 1.42‰, and their average δ~(15)N values were between -0.16 ± 1.34‰ and 14.99‰; the trophic levels ranged from 1.34 to 3.77. Cluster analysis showed that the food web among major species of Daya Bay could be classified into four trophic groups: suspended organic matter, primary producers and primary consumers, secondary consumers, and top predators. There were two main channels in the overall food chain: one was the grazing food chain, and the other was the detritus food chain. A. crassispina have a tendency to feed on detritus in August, when corals are scarce, macroalgae density is low, and the terrigenous materials present as particulate organic matter(POM) into Daya Bay follow a period of heavy rains. The main food resources of A. crassispina were: POM, sediment organic matter(SOM), the brown macroalgae Sargassum siliquastrum, bacillariophyceae(diatoms), zooplankton and phytoplankton Among these, POM was the most important food item for A. crassispina, accounting for 67.3% of its food consumption; the other food categories accounted for 9.7%, 9.3%, 6.7%, 3.7% and 3.3% of its food composition, respectively. Considerable competition exists between A. crassispina and the benthos, which suggests urgent efforts are needed to improve this ecological environment as well as to increase the seaweed biomass. Variations in the trophic levels were related to differences in the isotope baseline, and temporal and spatial variations. There was no significant correlation between shell diameter of A. crassispina and its feeding habits, perhaps because its food source is relatively singular. Thus, it is evident that feeding habits play an important role in population resource recovery and ecological niches of the system.
引文
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[9]Matsuoka N,Suzuki H.Genetic variation and differentiation in six local Japanese populations of the sea-urchin,Anthocidaris crassispina:Electrophoretic analysis of allozymes[J].Comp Biochem Physiol B:Comp Biochem,1989,92(1):1-7.
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[13]Nie Y K,Chen P M,Zhou Y B,et al.Preliminary study on feeding habits of Anthocidaris crassispina[J].South China Fisheries Science,2016,12(3):1-8.[聂永康,陈丕茂,周艳波,等.南方紫海胆摄食习性的初步研究[J].南方水产科学,2016,12(3):1-8.]
[14]Sun M Y,Gao X S.The influence of salinity on development and growth of the sea urchin[J].Journal of Fisheries of China,1991,15(1):72-76.[孙勉英,高绪生.盐度对大连紫海胆生长发育的影响[J].水产学报,1991,15(1):72-76.]
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[16]Post D M.Using stable isotopes to estimate trophic position:models,methods,and assumptions[J].Ecology,2002,83(3):703-718.
[17]Preciado I,Cartes J E,Punzón A,et al.Food web functioning of the benthopelagic community in a deep-sea seamount based on diet and stable isotope analyses[J].Deep Sea Res Part II:Topic Stud Oceanogry,2016:1-13
[18]Phillips Donald L.,Gregg Jillian W.Source partitioning using stable isotopes:coping with too many sources[J].Oecologia,2003,136(2):261-269.
[19].Trenzado C E,Hidalgo F,Villanueva D,et al.Study of the enzymatic digestive profile in three species of Mediterranean sea urchins[J].Aquaculture,2012,344:174-180.
[20]Kousuke Yatsuya,Nakahara Hiroyuki.Density,growth and reproduction of the sea urchin Anthocidaris crassispina(A.Agassiz)in two different adjacent habitats,the Sargassum area and Corallina area[J].Fish Sci,2004,70(2):233-240.
[21]Xie E Y,Jia C,Chen X L,et al.Seasonal variation in length,biomass and reproductive property of Sargassum naozhouense[J].Journal of Fisheries of China,2011,35(7):1015-1022.[谢恩义,贾柽,陈秀丽,等.硇洲马尾藻的繁殖特性及体长生物量的季节变动[J].水产学报,2011,35(7):1015-1022.]
[22]Wu Mei Lin,Wang You Shao.Using chemometrics to evaluate anthropogenic effects in Daya Bay,China[J].Estuar Coast Shelf Sci,2007,72(4):732-742.
[23]Li X L,Zhou Y P,Xia H Y.Intra-and Inter-annual Variabilities of Particulate Organic Matter in the Mirs Bay from 2000 to 2010[J].Environmental Science,2013,34(3):857-863.[李绪录,周毅频,夏华永.2000~2010年大鹏湾颗粒有机物的年变化和年际变化[J].环境科学,2013,34(3):857-863.]
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