SPIKE1 activates ROP GTPase signaling to modulate petal growth and shape in Arabidopsis thaliana

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• 英文论文题名：SPIKE1 activates ROP GTPase signaling to modulate petal growth and shape in Arabidopsis thaliana
• 论文作者：Huibo Ren ; Xie Dang ; Deshu Lin
• 英文论文作者：Huibo Ren ; Xie Dang ; Deshu Lin ; Haixia Institute of Science and Technology ; Fujian Agriculture and Forestry University
• 年：2016
• 作者机构：Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University;
• 英文论文关键词：ROP GTPase ; SPIKE1 ; petal shape ; microtubules ; Arabidopsis
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：Q945
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：841k
• 原文格式：D
• 会议级别：全国

摘要

The diverse morphological features displayed by plants,including shape and size of organs are regulated by the polarity and extension events of specially endowed cells. However,information regarding the functional proteins involved in the regulation of petal shape has not been extensively elucidated. Here,we investigate the mechanism that determines petal shape formation in Arabidopsis thaliana owing to its relatively short life span and structure simplicity. Current evidence showed that in Plants Rho(ROP) which is a kind of small GTPase protein that regulates the organization of cortical microtubules and vesicle trafficking under direct SPIKE1 activation,SPIKE1 belongs to DOCK family of proteins encoding a guanine nucleotide exchange factor(GEF). In this study,we respectively identified the interaction of SPIKE1 with ROP2 and ROP6 in our pull-down assays in vitro. Furthermore,analysis of rop2,rop6,rop2rop6 and rop2rop4irop6 mutants generated in this study exhibited lesser incidence of zigzag coupled with a reduction in the size of petal abaxial cell simultaneously,suggests ROP2,ROP4 and ROP6 synergistically modulate the development of petal shape,also,more transversely ordering microtubules were observed in late petal cells. More so,all the phenotypic characteristics observed in rop2rop4irop6 could be mirrored in spk1-4 point mutants. In conformity with these results,we posited that,ROP2,ROP4 and ROP6 activated by SPIKE1 and functions synergistically in modulating petal cell elongation and polarity in tandem with microtubules orientation and thus,influence growth and morphological development in Arabidopsis thaliana.
The diverse morphological features displayed by plants,including shape and size of organs are regulated by the polarity and extension events of specially endowed cells. However,information regarding the functional proteins involved in the regulation of petal shape has not been extensively elucidated. Here,we investigate the mechanism that determines petal shape formation in Arabidopsis thaliana owing to its relatively short life span and structure simplicity. Current evidence showed that in Plants Rho(ROP) which is a kind of small GTPase protein that regulates the organization of cortical microtubules and vesicle trafficking under direct SPIKE1 activation,SPIKE1 belongs to DOCK family of proteins encoding a guanine nucleotide exchange factor(GEF). In this study,we respectively identified the interaction of SPIKE1 with ROP2 and ROP6 in our pull-down assays in vitro. Furthermore,analysis of rop2,rop6,rop2rop6 and rop2rop4irop6 mutants generated in this study exhibited lesser incidence of zigzag coupled with a reduction in the size of petal abaxial cell simultaneously,suggests ROP2,ROP4 and ROP6 synergistically modulate the development of petal shape,also,more transversely ordering microtubules were observed in late petal cells. More so,all the phenotypic characteristics observed in rop2rop4irop6 could be mirrored in spk1-4 point mutants. In conformity with these results,we posited that,ROP2,ROP4 and ROP6 activated by SPIKE1 and functions synergistically in modulating petal cell elongation and polarity in tandem with microtubules orientation and thus,influence growth and morphological development in Arabidopsis thaliana.

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