NCP1/At MOB1A plays key roles in auxin-mediated Arabidopsis development

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• 英文论文题名：NCP1/At MOB1A plays key roles in auxin-mediated Arabidopsis development
• 论文作者：Xiaona Cui ; Zhiai Guo ; Lizhen Song ; Yanli Wang ; Youfa Cheng
• 英文论文作者：Xiaona Cui ; Zhiai Guo ; Lizhen Song ; Yanli Wang ; Youfa Cheng ; Key Laboratory of Plant Molecular Physiology ; Institute of Botany ; Chinese Academy of Sciences ; University of Chinese Academy of Sciences
• 年：2016
• 作者机构：Key Laboratory of Plant Molecular Physiology,Institute of Botany,Chinese Academy of Sciences;University of Chinese Academy of Sciences;
• 英文论文关键词：NCP1/At MOB1A ; Hippo pathway ; auxin ; embryogenesis
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：Q945
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：852k
• 原文格式：D
• 会议级别：全国

摘要

MOB1 protein is a core component of the Hippo signaling pathway in animals,where it is involved in controlling tissue growth and tumor suppression. Plant MOB1 proteins display high sequence homology to animal MOB1 proteins,but little is known regarding their role in plant growth and development. Herein we report the critical roles of Arabidopsis MOB1(At MOB1A) in auxin-mediated development in Arabidopsis. We found that loss-of-function mutations in At MOB1 A completely eliminated the formation of cotyledons when combined with mutations in PINOID(PID),which encodes a Ser/Thr protein kinase that participates in auxin signaling and transport. We show ed that atmob1 a was fully rescued by its Drosophila counterpart,suggesting functional conservation. The atmob1 a pid double mutants phenocopied several well-characterized mutant combinations that are defective in auxin biosynthesis or transport. Moreover,we demonstrated that atmob1 a greatly enhanced several other known auxin mutants,suggesting that At MOB1 A plays a key role in auxinmediated plant development. The atmob1 a single mutant displayed defects in early embryogenesis and had shorter root and smaller flowers than wild type plants. At MOB1 A is uniformly expressed in embryos and suspensor cells during embryogenesis,consistent with its role in embryo development. At MOB1 A protein is localized to nucleus,cytoplasm,and associated to plasma membrane,suggesting that it plays roles in these subcellular localizations. Furthermore,we showed that disruption of At MOB1 A led to a reduced sensitivity to exogenous auxin. Our results demonstrated that At MOB1 A plays an important role in Arabidopsis development by promoting auxin signaling.
MOB1 protein is a core component of the Hippo signaling pathway in animals,where it is involved in controlling tissue growth and tumor suppression. Plant MOB1 proteins display high sequence homology to animal MOB1 proteins,but little is known regarding their role in plant growth and development. Herein we report the critical roles of Arabidopsis MOB1(At MOB1A) in auxin-mediated development in Arabidopsis. We found that loss-of-function mutations in At MOB1 A completely eliminated the formation of cotyledons when combined with mutations in PINOID(PID),which encodes a Ser/Thr protein kinase that participates in auxin signaling and transport. We show ed that atmob1 a was fully rescued by its Drosophila counterpart,suggesting functional conservation. The atmob1 a pid double mutants phenocopied several well-characterized mutant combinations that are defective in auxin biosynthesis or transport. Moreover,we demonstrated that atmob1 a greatly enhanced several other known auxin mutants,suggesting that At MOB1 A plays a key role in auxinmediated plant development. The atmob1 a single mutant displayed defects in early embryogenesis and had shorter root and smaller flowers than wild type plants. At MOB1 A is uniformly expressed in embryos and suspensor cells during embryogenesis,consistent with its role in embryo development. At MOB1 A protein is localized to nucleus,cytoplasm,and associated to plasma membrane,suggesting that it plays roles in these subcellular localizations. Furthermore,we showed that disruption of At MOB1 A led to a reduced sensitivity to exogenous auxin. Our results demonstrated that At MOB1 A plays an important role in Arabidopsis development by promoting auxin signaling.

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