芹菜对镉、铅处理的生理响应及抗氧化酶基因表达特性分析

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• 英文论文题名：Physiological Responsesto Cd~(2+),Pb~(2+) Pollution and Expression Analysis of Antioxidative Genes inCelery
• 论文作者：肖旭峰 ; 吴才君 ; 解庆娓 ; 范淑英
• 英文论文作者：XIAO Xu-feng ; WU Cai-jun ; XIE Qing-wei ; FAN Shu-ying ; College of Agronomy ; Jiangxi Agricultural University
• 年：2016
• 作者机构：江西农业大学农学院;
• 论文关键词：Cd ; Pb ; 复合污染 ; 芹菜 ; 基因表达
• 英文论文关键词：Cd ; Pb ; combined pollution ; celery(Apium graveolens L.) ; gene expression
• 会议召开时间：2016-04-15
• 会议录名称：2016全国设施园艺产业发展与安全高效栽培技术交流会论文汇编
• 语种：中文
• 分类号：S636.3
• 学会代码：EGYP
• 会议名称：2016全国设施园艺产业发展与安全高效栽培技术交流会
• 会议地点：中国四川成都
• 主办单位：中国园艺学会、中国农用塑料应用技术学会设施园艺专业委员会
• 学会名称：中国园艺学会
• 页数：8
• 文件大小：1036k
• 原文格式：O
• 会议级别：全国

摘要

为探讨镉(Cd)、铅(Pb)胁迫对芹菜抗逆生理代谢及抗氧化酶基因农达特性的影响,采用营养液培试验研究了Cd~(2+)、Pb~(2+)单一及复合污染对芹菜叶片叶绿素、丙二醛(MDA)及抗氧化酶(SOD、POD)活性的影响,并用qRT-PCR技术检测了重金属离子处理后芹菜根尖细胞内抗氧化酶相关基因的表达变化。结果表明:6-8mg·L~(-1)Cd和60-80mg·L~(-1)Pb单一及复合处理对芹菜整个生长期内叶绿素、MDA、GSH含量及SOD、POD活性的影响均达到极显著性效应(p<0.01):低浓度Cd、Pb及Cd-Pb复合处理对叶绿素合成具有促进效应,而高浓度、长时间胁迫时,叶绿素含量受到明显抑制;Cd、Pb单一及复合胁迫对MDA含量及SOD、POD活性的影响均为Cd-Pb交互>Cd>Pb,但当单一Cd≥4 mg·L~(-1)时,芹菜叶绿素含量小于Cd-Pb交互及单一Pb胁迫;Cd、Pb对芹菜叶POD和SOD酶活性的影响相似,均表现为"低促高抑"的特点;重金属浓度越高,芹菜叶片内MDA及GSH的含量增加,可在一定程度上缓解重金属污染对芹菜植株的毒害作用;Cd~(2+)、Pb~(2+)单一及复合处理下Cat、Gpx、Mn-sod及Apx表达模式基本一致,其表达量的降低幅度为Cd-Pb交互>Cd>Pb。Cd、Pb胁迫对芹菜的生理和抗氧化系统均造成一定的损伤,二者具有协同性,该结果为江西省鄱阳湖区芹菜的安全生产提供理论依据。
In order to supply references for ensuring vegetable production safety,a hydroponic experiment was conducted on the effects of cadmium(Cd) and lead(Pb) single and combined stresses on contents of chlorophyll(Chi),malondialdehyde(MDA),glutathione(GSH),and antioxidant enzyme activities(superoxidedismutase,SOD,peroxidase,POD),and tested the expression of anti-oxidative genes with qRT-PCRin celery(Apium graveolens).The results showed:(1)Compared with CK,the influences of Cd(6-8mg·L~(-1)),Pb(60-80mg·L~(-1)) single andcombined stress on contents of Chl,GSH,MDA and activities of SOD,POD in the leaves were very sigmficantdifferences(p<0.01)during the whole growth cycle of celery.(2) Content of Chlwasincreased under the low concentrations of Cd,Pband Cd-Pbcombined stress,but decreased under high concentrations and long time.(3) The effects of Cd,Pb onMDA,GSH,SOD and PODshowed Cd-Pb combined>Cd>Pb,but when concentration of Cd>4 mg·L~(-1),the destructive effects on Chlshowed Cd>Cd-Pb>Pb.(4) The effects of Cd,Pb on activities of POD and SOD in celery leafwere similar.Both existed hormesis effect.(5) Contents of MDA and GSHwere promoted gradually with heavy metal concentration increasing,which could alleviate Cd and Pb toxicity on celery seedlings.(6) Expression patterns of Cat、Gpx、Mn-sod andApxhad the similar results under Cd,Pb and Cd-Pb,and such reduction was Cd-Pb combined>Cd>Pb.In a conclusion,this study demonstrated that physiological system and antioxidant system of celery were damaged under Cd and Pb stress.

引文

[1]Cao H B,Chen J J,Zhang J,Zhang Hui,Qiao Li.Heavy metals in rice and garden vegetables and their potential health risks to inhabitants in the vicinity of an industrial zone in Jiangsu,China[J].Journal of Environmental Sciences,2010,22:1792-1798.
    [2]Landis W G,Yu M.Introduction to environmental toxicology:impacts of chemicals upon ecological systems[J].New York:2nd.Lew is Publishers,2009,211-221.
    [3]Silva A L,Barrocas P G,Jacob S C,Moreira J C.Dietary intake and health effects of selected toxic elements[J].Brazil Journal of Plant Physiology,2005,17:79-93.
    [4]Renella G,Ortiaoza A L R,Landi L,Nannipieri P.Additive effects of copper and zinc on cadmium toxicity on phosphatase activities and ATP content of soil as estimated by the ecological dose(ED50)[J].Soil Biology&Biochemisity,2003,35(9):1203-1210.
    [5]杨菲,吴琦,季辉,张卫建.上壤重金属Pb和Cd在小白菜中的富集特征及产地环境安全临界值[J].中国农学通报,2011,27(13):194-198.
    [6]黎佳佳,胡红青,付庆灵,吕意.Cd、Pb单一与复合污染对辣椒生物量及重金属残留的影响[J].农业环境科学学报,2006,25(1):49-53.
    [7]陈新红,叶玉秀,庞闰瑾.镉、铅及其互作对黄瓜种子发芽及幼根生长的影响[J].北方园艺,2009,5:13-16.
    [8]刘仕哲.现代农业无土栽培技术[M].北京:中国农业出版社,2001.
    [9]张志良,瞿伟菁.植物生理学实验指导第三版[M].北京:高等教育出版社,2004.
    [10]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
    [11]李博文,谢建治,郝晋珉.不同蔬菜对潮褐土镉铅锌复合污染的吸收效应研究[J].农业环境科学学报,2003,22(3):286-288.
    [12]刘柿良,杨容孑,马明东,蒋潘,赵燕.土壤镉胁迫对龙葵幼苗生长及生理特性的影响[J].农业环境科学学报,2015,34(2):240-248.
    [13]Sakuraba Y,Rahman M L,Cho S H,Kim Y S,Koh H J.The rice faded green leaf lo-cus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions[J].PlantJ,2013,74(1):122-133.
    [14]李佩华.铅、镉胁迫对马铃薯叶绿素含量及细胞超微结构的影响[J].湖北农业科学,2015,54(16):3974-3978.
    [15]陈怀满,郑春荣.交互作用对植物生长和元素循环的影响[J].土壤学进展,1994,22(1):47-49.
    [16]祖艳群,李元,陈海燕,陈建军,Marianne G.蔬菜中铅镉铜锌含量的影响因素研究.农业环境科学学报,2003,22(3):289-292.
    [17]赵仕诚.镉诱导植物氧化代谢与钙信使的变化及分子机理[D].北京:中国农业科学院博士论文,2008:
    [18]Mishra S,Srivastava S,Tripathi R D,Govindarajan R,Kuriakose S V.Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L[J].Plant Physiol Biochem,2006,44(2):25-37.
    [19]刘俊祥,魏树强,翟飞飞,李伟,周晓星,孙振元.Cd~(2+)胁迫下多年生黑麦草的生长与生理响应[J].核农学报,2015,29(3):587-594.
    [20]郭天荣,陈丽萍,冯其芳,戚志伟,沈佳辉.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576.
    [21]Benavides M,Gallego S,Tomaro M.Cadmium toxicity in plants[J].Braz J Plant Physiol,2005,17(1):21-34.
    [22]Kovcik J,Babula P,Klejdus B,Hedbavny J,Jarosova M.Unexpected behavior of some nitric oxide modulators under cadmium excess in plant tissue[J].PloS ONE,2014,9:e91685.DOI:10.1371/journal.pone.0091685.
    [23]王亭亭,王中浩,熊方建,曹昊昊,刘志斌,夏传琴,王健美.铀胁迫对油菜的生长及抗氧化酶的影响[J].四川大学学报,2014,15(1):171-176.
    [24]张巧丽,刘宛,李培军,韩艳萍.镉胁迫对拟南芥幼苗错配修复基因表达的影响[J].生态学杂志,2008,27(7):1173-1180.
    [25]Miesch J,Tschiraedbalshir M,Barlocher F.Heavy metals and thiol compounds in Mucor racemosus and Articulospora tetracladia[J].Mycological Research,2001,105:883-889.
    [26]高俊杰,秦爱国,于贤昌.低温胁迫下嫁接对黄瓜叶片SOD和CAT基因表达与活性变化的影响[J].应用生态学报,2009,20:213-217.
    [27]张玉秀,金玲,冯珊珊,刘金光,柴团耀.镉对镉超累积植物龙葵抗氧化酶活性及基因表达的影响[J].中国科学院研究生院学报2013,30(1):1 1-17.