浙江地方柚类种质资源遗传关系的SSR标记研究
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- 英文论文题名：Genetic diversity of pummelo germplasms in Zhejiang province based on SSR molecular markers
- 论文作者：刘冬峰 ; 陈巍 ; 林绍生 ; 徐文荣 ; 郭秀珠 ; 黄品湖
- 英文论文作者：LIU Dong-feng ; CHEN Wei ; LIN Shao-sheng ; XU Wen-rong ; GUO Xiu-zhu ; HUANG Pin-hu ; Zhejiang Insititute of Subtropical Crops ; Jiangsu Polytechnic College Agriculture and Forestry
- 年：2016
- 作者机构：浙江省亚热带作物研究所;江苏农林职业技术学院;
- 论文关键词：柚 ; 种质资源 ; SSR ; 遗传多样性 ; 种质鉴定
- 英文论文关键词：pummelo ; germplasm resource ; SSR ; genetic diversity ; germplasm characterization
- 会议召开时间：2016-07-23
- 会议录名称：第五届全国现代果业标准化示范区创建暨果树优质高效生产技术交流会论文汇编
- 语种：中文
- 分类号：S666.3
- 学会代码：EGYP
- 会议名称：第五届全国现代果业标准化示范区创建暨果树优质高效生产技术交流会
- 会议地点：中国新疆乌鲁木齐
- 主办单位：中国园艺学会、中国园艺学会果树专业委员会
- 学会名称：中国园艺学会
- 页数：10
- 文件大小：666k
- 原文格式：O
- 会议级别：全国
摘要
浙江省是我国柚类遗传多样性的主要分布区之一,本研究搜集本省18份地方柚类种质资源利用24对柑橘SSR引物进行遗传多样性分析并构建种质鉴定图,旨在为地方资源的搜集保护和合理利用提供参考。24对引物共检测到92个等位变异位点,变异范围在1-7之间,平均每对SSR引物可检测到3.8个等位位点;24个SSR位点的观察杂合度、期望杂合度和多态性信息含量分别为0.49、0.51和0.45;18份柚类种质资源两两间的遗传相似性系数介于0.55-0.94之间,平均为0.71,表明浙江地方柚类种质资源具有丰富的遗传多样性,遗传背景较复杂。UPGMA聚类分析将供试材料分成A1-A3、B1、C1和D1共6个组群,其中A1组包括四季柚、佛香柚和麻步文旦等7份果肉颜色为黄色或粉红色的种质,A2组包括永嘉红心柚2号、青田红心柚和木兰柚3份果肉颜色为浅红色的种质,A3组包括处红柚、永嘉红心柚1号、古磉柚和官南红柚4份果肉颜色为红色的种质,官南土柚和文成红心柚自成B1和C1组,常山胡柚和槾文柑2个杂种柚类型聚为D1组。除少数个别材料外,浙江地方柚类种质资源有按果肉颜色优先聚类的趋势,但是黄色和粉红色的种质资源间并未得到明显的区分,说明SSR分子聚类结果与果实表型基本吻合。另外,利用AG14、CAG01、CMS-16和CMS-26 4对SSR引物可快速准确地将18份供试种质鉴别开来,以此构建了浙江省地方柚类资源的种质鉴定图,可应用于种苗真实性鉴定。
It is one of the main distribution area of pummelo genetic diversity in Zhejiang province.In this study,twenty-four simple sequence repeat(SSR) primers developed in Citrus were used to detect the genetic diversity of 18 local pummelo germplasms collected in Zhejiang province,and to construct germplasm characterization map,which would contribute to the local resources protection and subsequent rational utilization.A total of 92 alleles were detected among twenty-four SSR loci,ranged from one to seven and with an average of 3.8 alleles per locus.The mean observed and expected heterozygosity values were respectively 0.49 and 0.51,and the average polymorphism information content of 24 SSR loci was0.45.The genetic similarity coefficients between genotypes ranged from 0.55 to 0.94 and with an average of 0.71.The above results showed a rich genetic polymorphism and complicated genetic background among Zhejiang pummelo germplasm resources.The UPGMA dedrogram based on the genetic similarity coefficients grouped 18 pummelo accessions to six major groups at the coefficient of 0.71 as follows:Sijiyou,Foxiangyou,Mabu-wendan and other seven yellow or pink pulp colour cultivars were clustered to A1 group;the three pale red cultivars including Yongjia-hongxinyou 2,Qingtian-hongxinyou and Mulanyou were clustered to A2 group;the four red cultivars including Chuhongyou,Yongjia-hongxinyou 1,Gusangyou and Guannan-hongyou were clustered to A3 group;Guannan-tuyou and Wencheng-hongxinyou was separately clustered to B1 and C1;and the two hybrids from the cross of pummelo and other citrus were clustered to D1 group.The results showed that except for a few germplasms most local pummelo cultivars were clustered preferentially related to their pulp colours,however,the germplasms between yellow and pink colours were not totally distingushed,which indicated the SSR molecular dendrogram approximately agreed with the morphological characters.Moreover,four SSR primers AG14,CAG01,CMS-16 and CMS-26 could rapidly and accurately separate all the tested materials apart,and accordingly a germplasm characterization map of Zhejiang province was constructed,which could be applied to seedling authenticity characterization.
It is one of the main distribution area of pummelo genetic diversity in Zhejiang province.In this study,twenty-four simple sequence repeat(SSR) primers developed in Citrus were used to detect the genetic diversity of 18 local pummelo germplasms collected in Zhejiang province,and to construct germplasm characterization map,which would contribute to the local resources protection and subsequent rational utilization.A total of 92 alleles were detected among twenty-four SSR loci,ranged from one to seven and with an average of 3.8 alleles per locus.The mean observed and expected heterozygosity values were respectively 0.49 and 0.51,and the average polymorphism information content of 24 SSR loci was0.45.The genetic similarity coefficients between genotypes ranged from 0.55 to 0.94 and with an average of 0.71.The above results showed a rich genetic polymorphism and complicated genetic background among Zhejiang pummelo germplasm resources.The UPGMA dedrogram based on the genetic similarity coefficients grouped 18 pummelo accessions to six major groups at the coefficient of 0.71 as follows:Sijiyou,Foxiangyou,Mabu-wendan and other seven yellow or pink pulp colour cultivars were clustered to A1 group;the three pale red cultivars including Yongjia-hongxinyou 2,Qingtian-hongxinyou and Mulanyou were clustered to A2 group;the four red cultivars including Chuhongyou,Yongjia-hongxinyou 1,Gusangyou and Guannan-hongyou were clustered to A3 group;Guannan-tuyou and Wencheng-hongxinyou was separately clustered to B1 and C1;and the two hybrids from the cross of pummelo and other citrus were clustered to D1 group.The results showed that except for a few germplasms most local pummelo cultivars were clustered preferentially related to their pulp colours,however,the germplasms between yellow and pink colours were not totally distingushed,which indicated the SSR molecular dendrogram approximately agreed with the morphological characters.Moreover,four SSR primers AG14,CAG01,CMS-16 and CMS-26 could rapidly and accurately separate all the tested materials apart,and accordingly a germplasm characterization map of Zhejiang province was constructed,which could be applied to seedling authenticity characterization.
引文
Amar M H,Biswas M K,Zhang Zong-wen,Guo Wen-wu.2011.Exploitation of SSR,SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection.Scientia Horticulturae,128(3):220-227.
Bakhshipour H,Golein B,Mehregan I,Saadatmand S.2015.Simple sequence repeat polymorphism in Iranian Citrus germplasm including unknown variants.Agricultural Research.4(2):152-159.
Cao Li-xin.2012.The investigation of pummelo germplasms and the origin analysis of Majiayou Guangfeng,Jinagxi Province[Master Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)曹立新,2012.江西省广丰县柚资源调查与马家柚起源分析[硕士论文].武汉:华中农业大学
Chen Zhen-guang,Lai Zhong-xiong.1993.Introduction and research of pemmelo germplasm in China.Journal of Fujian Agricultural University(Nature Science Edition),22(3):290-295.(in Chinese)陈振光,赖钟雄,1993.中国柚的种质资源及其研究.福建农学院学报(自然科学版),22(3):290-295.
Ding Jian,Deng Xiu-xin,Zhang Hong-yan,Cheng Yun-jiang,Xu Qiang,Liu Qing.2009.Somaclonal variation with early j'uice-sac granulation obtained by inter-specific protoplast fusion in Citrus.Journal of Horticultural Science&Biotechnology,84(5):567-573.
Ghorabaie H R R,Ghazvini R F,Golein B,Nabipour A R.2010.Identification of some Citrus accessions in a Citrus germplasm utilizing simple sequence repeat markers(SSRs).Horticulture Environment and Biotechnology,51(4):343-347.
Han Guo-hui,Xiang Su-qiong,Wang Wei-xing,Wei Xu,He Bo,Li Xiao-lin,Liang Guo-lu.2010.Identification and genetic diversity of hybrid progenies from Shatian pummelo by SSR.Scientia Agriculture Sinica,43(22):4678-4686.(in Chinese)韩国辉,向素琼,汪卫星,魏旭,何波,李晓林,梁国鲁,2010.沙田柚杂交后代群体的SSR鉴定与遗传多样性分析.中国农业科学,43(22):4678-4686.
Huang Wei-j'ie,Pan Xiang-qiang.2011.The observation of Mulan pummelo biological characteristics.Zhejiang Ganju,28(4):21-22.(in Chinese)黄为杰,潘孝强,2011.木兰柚生物学特性的观察.浙江柑橘,28(4):21-22.
Lee Tan-cha,Zhong Pei-juan,Chang Pai-tsang.2015.The effects of preharvest shading and postharvest storage temperatures on the quality of'Ponkan'(Citrus reticulata Blanco)mandarin fruits.Scientia Horticulturae,188(0):57-65.
Liang Wu-jun,Xie Kai-dong,Xie Zong-zhou,Xu Qiang,Yi Hua-lin,Guo Wen-wu.2015.Exploitation of polyploids from open-poilinated triploid grapefruit progenies and their genetic identification by SSR molecular markers.Journal of Fruit Science,32(1):13-18.(in Chinese)粱武军,解凯东,谢宗周,徐强,伊华林,郭文武.2015.三倍体葡萄柚实生后代多倍体的发掘与SSR遗传鉴定.果树学报,32(1):13-18.
Lin Shao-sheng,Liu Dong-feng,Guo Xiu-zhu,Huang Pin-hu,Xu Wen-rong,Chen Wei.2015.Analyses of genetic diversity of pummelo(Osbeck)in Zhejiang gerplasms by SRAP markers.Molecular Plant Breeding,13(3):562-566.(in Chinese)林绍生,刘冬峰,郭秀珠,黄品湖,徐文荣,陈巍,2015.浙江柚类地方资源srap标记的遗传多样性分析.分子植物育种,13(3):562-566.
Liu yong.2015.Molecular phylogenetic analysis and core collection construction using SSR and AFLP markers in ummelo[Ph.D.Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)刘勇.2015.柚类资源分子系统学及其核心种质构建研究[博士论文].武汉:华中农业大学.
Pal D,Malik S K,Kumar S,Choudhary R.,Sharma K C,Chaudhury R.2013.Genetic variability and relationship studies of mandarin(Citrus reticulata Blanco)using morphological and molecular markers.Agricultural Research,2(3):236-245.
Shi Kai-ming.2014.Analysis of genetic diversity of Citrus grandis Osbeck in Western Hubei by RAPD and ISSR markers[Master Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)石开明.2004.利用RAPD、ISSR标记分析鄂西地区柚种质资源遗传多样性[硕士论文].武汉:华中农业大学.
Sui Li-yun,Liu Yi-fei,Hung Hong-wen.2013.Genetic diversity of red-fleshed kiwifruit germplasm based on fruit traits and AFLP markers.Acta Horticulturae Sinica,40(5):859-868.(in Chinese)岁立云,刘义飞,黄宏文,2013.红肉猕猴桃种质资源果实性状及AFLP遗传多样性分析.园艺学报,40(5):859-868.
Tang Jia-le,Huang Chun-hui,Wu Han,Lang Bin-bin,Qu Xue-yan,Xu Xiao-biao.2014.Genetic diversity of wild Actinidia eriantha germplasm based on fruit traits and ssr markers.Acta Horticulturae Sinica,41(6):1198-1206.(in Chinese)汤佳乐,黄春辉,吴寒,郎彬彬,曲雪艳,徐小彪,2014.野生毛花猕猴桃果实表型性状及ssr遗传多样性分析.园艺学报,41(6):1198-1206.
Wang Fu-sheng,Jiang Dong.2010.Studies on genetic background of important germplasm resources among citrus based on cpSSR and EST-SSR marker.Acta Horticulturae,Sinica 37(3):465-474.(in Chinese)王福生,江东.2010.应用cpSSR和EST-SSR标记进行柑橘特异种质资源遗传背景研究.园艺学报,37(3):465-474.
Wang Xian-you,Wang Ping,Qi Yi-ping,Zhou Chen-ping,Yang Lin-tong,LiaoXin-ya,Wang Liu-qing,Zhu Dong-huang,Chen Li-song.2014.Effects of granulation on organic acid metabolism and its relation to mineral elements in Citrus grandis juice sacs.Food Chemistry,145:984-990.
Wu Jia-ling,Pan Teng-fei,Guo Zhi-xiong,Pan Dong-ming.2014.Specific lignin accumulation in granulated juice sacs of Citrus maxima.Journal of Agricultural and Food Chemistry,62(50):12082-12089.
Xu Juan,Deng Xiu-xin.2002.Red juice sac of Citrus and its main pigments.Journal of Fruit Science.19(5):307-313.(in Chinese)徐娟,邓秀新.2002.柑橘类果实汁胞的红色现象及其呈色色素.果树学报,19(5):307-313.
Yang Ying-hua.2009.The development of SRAP marker and identification of pummelo germplasm diversity in Hunan[Master Dissertation].Changsha:Hunan Agricultural university,(in Chinese)杨迎花,2009.利用SRAP标记对湖南柚资源遗传多样性的分析[硕士论文].长沙:湖南农业大学.
Zane L,Bargelloni L,Patarnello T.2002.Strategies for microsatellite isolation:A review.Molecular Ecology,11(1):1-16.
Zhang Tai-ping,Peng Shao-lin,Wang Zheng-feng,Chen Bi-chen.2001.Introduction to the study and protection of pomelo germplsm.Ecologic Science,20(3):8-13.(in Chinese)张太平,彭少麟,王峥峰,陈碧琛,2001.柚类种质资源研究与保护概况.生态科学,20(3):8-13.
Zhen Yi-qi,Li Zuo-zhou,Huang Hong-wen,Wang Ying.2004.Molecular characterization of Kiwifruit(Actinidia)cultivars and selections using SSR markers.Journal of the American Society for Horticultural Science,129(3):374-382.
Bakhshipour H,Golein B,Mehregan I,Saadatmand S.2015.Simple sequence repeat polymorphism in Iranian Citrus germplasm including unknown variants.Agricultural Research.4(2):152-159.
Cao Li-xin.2012.The investigation of pummelo germplasms and the origin analysis of Majiayou Guangfeng,Jinagxi Province[Master Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)曹立新,2012.江西省广丰县柚资源调查与马家柚起源分析[硕士论文].武汉:华中农业大学
Chen Zhen-guang,Lai Zhong-xiong.1993.Introduction and research of pemmelo germplasm in China.Journal of Fujian Agricultural University(Nature Science Edition),22(3):290-295.(in Chinese)陈振光,赖钟雄,1993.中国柚的种质资源及其研究.福建农学院学报(自然科学版),22(3):290-295.
Ding Jian,Deng Xiu-xin,Zhang Hong-yan,Cheng Yun-jiang,Xu Qiang,Liu Qing.2009.Somaclonal variation with early j'uice-sac granulation obtained by inter-specific protoplast fusion in Citrus.Journal of Horticultural Science&Biotechnology,84(5):567-573.
Ghorabaie H R R,Ghazvini R F,Golein B,Nabipour A R.2010.Identification of some Citrus accessions in a Citrus germplasm utilizing simple sequence repeat markers(SSRs).Horticulture Environment and Biotechnology,51(4):343-347.
Han Guo-hui,Xiang Su-qiong,Wang Wei-xing,Wei Xu,He Bo,Li Xiao-lin,Liang Guo-lu.2010.Identification and genetic diversity of hybrid progenies from Shatian pummelo by SSR.Scientia Agriculture Sinica,43(22):4678-4686.(in Chinese)韩国辉,向素琼,汪卫星,魏旭,何波,李晓林,梁国鲁,2010.沙田柚杂交后代群体的SSR鉴定与遗传多样性分析.中国农业科学,43(22):4678-4686.
Huang Wei-j'ie,Pan Xiang-qiang.2011.The observation of Mulan pummelo biological characteristics.Zhejiang Ganju,28(4):21-22.(in Chinese)黄为杰,潘孝强,2011.木兰柚生物学特性的观察.浙江柑橘,28(4):21-22.
Lee Tan-cha,Zhong Pei-juan,Chang Pai-tsang.2015.The effects of preharvest shading and postharvest storage temperatures on the quality of'Ponkan'(Citrus reticulata Blanco)mandarin fruits.Scientia Horticulturae,188(0):57-65.
Liang Wu-jun,Xie Kai-dong,Xie Zong-zhou,Xu Qiang,Yi Hua-lin,Guo Wen-wu.2015.Exploitation of polyploids from open-poilinated triploid grapefruit progenies and their genetic identification by SSR molecular markers.Journal of Fruit Science,32(1):13-18.(in Chinese)粱武军,解凯东,谢宗周,徐强,伊华林,郭文武.2015.三倍体葡萄柚实生后代多倍体的发掘与SSR遗传鉴定.果树学报,32(1):13-18.
Lin Shao-sheng,Liu Dong-feng,Guo Xiu-zhu,Huang Pin-hu,Xu Wen-rong,Chen Wei.2015.Analyses of genetic diversity of pummelo(Osbeck)in Zhejiang gerplasms by SRAP markers.Molecular Plant Breeding,13(3):562-566.(in Chinese)林绍生,刘冬峰,郭秀珠,黄品湖,徐文荣,陈巍,2015.浙江柚类地方资源srap标记的遗传多样性分析.分子植物育种,13(3):562-566.
Liu yong.2015.Molecular phylogenetic analysis and core collection construction using SSR and AFLP markers in ummelo[Ph.D.Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)刘勇.2015.柚类资源分子系统学及其核心种质构建研究[博士论文].武汉:华中农业大学.
Pal D,Malik S K,Kumar S,Choudhary R.,Sharma K C,Chaudhury R.2013.Genetic variability and relationship studies of mandarin(Citrus reticulata Blanco)using morphological and molecular markers.Agricultural Research,2(3):236-245.
Shi Kai-ming.2014.Analysis of genetic diversity of Citrus grandis Osbeck in Western Hubei by RAPD and ISSR markers[Master Dissertation].Wuhan:Huazhong Agricultural university,(in Chinese)石开明.2004.利用RAPD、ISSR标记分析鄂西地区柚种质资源遗传多样性[硕士论文].武汉:华中农业大学.
Sui Li-yun,Liu Yi-fei,Hung Hong-wen.2013.Genetic diversity of red-fleshed kiwifruit germplasm based on fruit traits and AFLP markers.Acta Horticulturae Sinica,40(5):859-868.(in Chinese)岁立云,刘义飞,黄宏文,2013.红肉猕猴桃种质资源果实性状及AFLP遗传多样性分析.园艺学报,40(5):859-868.
Tang Jia-le,Huang Chun-hui,Wu Han,Lang Bin-bin,Qu Xue-yan,Xu Xiao-biao.2014.Genetic diversity of wild Actinidia eriantha germplasm based on fruit traits and ssr markers.Acta Horticulturae Sinica,41(6):1198-1206.(in Chinese)汤佳乐,黄春辉,吴寒,郎彬彬,曲雪艳,徐小彪,2014.野生毛花猕猴桃果实表型性状及ssr遗传多样性分析.园艺学报,41(6):1198-1206.
Wang Fu-sheng,Jiang Dong.2010.Studies on genetic background of important germplasm resources among citrus based on cpSSR and EST-SSR marker.Acta Horticulturae,Sinica 37(3):465-474.(in Chinese)王福生,江东.2010.应用cpSSR和EST-SSR标记进行柑橘特异种质资源遗传背景研究.园艺学报,37(3):465-474.
Wang Xian-you,Wang Ping,Qi Yi-ping,Zhou Chen-ping,Yang Lin-tong,LiaoXin-ya,Wang Liu-qing,Zhu Dong-huang,Chen Li-song.2014.Effects of granulation on organic acid metabolism and its relation to mineral elements in Citrus grandis juice sacs.Food Chemistry,145:984-990.
Wu Jia-ling,Pan Teng-fei,Guo Zhi-xiong,Pan Dong-ming.2014.Specific lignin accumulation in granulated juice sacs of Citrus maxima.Journal of Agricultural and Food Chemistry,62(50):12082-12089.
Xu Juan,Deng Xiu-xin.2002.Red juice sac of Citrus and its main pigments.Journal of Fruit Science.19(5):307-313.(in Chinese)徐娟,邓秀新.2002.柑橘类果实汁胞的红色现象及其呈色色素.果树学报,19(5):307-313.
Yang Ying-hua.2009.The development of SRAP marker and identification of pummelo germplasm diversity in Hunan[Master Dissertation].Changsha:Hunan Agricultural university,(in Chinese)杨迎花,2009.利用SRAP标记对湖南柚资源遗传多样性的分析[硕士论文].长沙:湖南农业大学.
Zane L,Bargelloni L,Patarnello T.2002.Strategies for microsatellite isolation:A review.Molecular Ecology,11(1):1-16.
Zhang Tai-ping,Peng Shao-lin,Wang Zheng-feng,Chen Bi-chen.2001.Introduction to the study and protection of pomelo germplsm.Ecologic Science,20(3):8-13.(in Chinese)张太平,彭少麟,王峥峰,陈碧琛,2001.柚类种质资源研究与保护概况.生态科学,20(3):8-13.
Zhen Yi-qi,Li Zuo-zhou,Huang Hong-wen,Wang Ying.2004.Molecular characterization of Kiwifruit(Actinidia)cultivars and selections using SSR markers.Journal of the American Society for Horticultural Science,129(3):374-382.
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