龙泉煤层气田产甲烷菌群富集的厌氧产气中试研究
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摘要
为探讨龙泉煤层气田产甲烷菌群降解烟煤产甲烷的潜力,研究富集并驯化了龙泉煤层气田产甲烷菌群;以龙泉矿烟煤为母质,开展产甲烷菌群降解煤产气的实验室研究以及中试研究。结果表明,龙泉煤层产甲烷菌群经富集驯化后,降解烟煤产甲烷的浓度由8.2%上升至19.0%,且产气周期缩短。中试试验中,产甲烷浓度最高达27.06%,产甲烷速率达0.159 m L/(g·d);且在中试发酵产气的衰退期,向发酵罐内添加酵母粉、氮源、磷盐、镁盐、维生素、微量元素,可重新激活产甲烷菌群的活性,使其降解烟煤产甲烷的浓度回升。由此可见,富集驯化龙泉煤层产甲烷菌群可显著提高其降解烟煤产甲烷的能力;在发酵产气衰退期补加营养物质,可以活化产甲烷菌群,恢复其降解烟煤产甲烷的能力。
In order to explore the potential of methanogenic bacteria in Longquan coalbed methane field to degrade methane production in bituminous coal,the enrichment and domestication of methanogens in Longquan coalbed methane field was studied.The laboratory study as well as pilot study on the degradation of coal gas production by methanogens was carried out with Longquan mine bituminous coal as parent material.The results showed that the concentration of methanogenic degradation of bituminous coal increased from 8.2% to 19% after enrichment and domestication of methanogens,the cycle of gas production stability was shortened.In the pilot test,the methanogenic concentration was up to 27.06%,and methane production rate was 0.159 m L/( g·d). By supplementing yeast powder,nitrogen sources,phosphates,magnesium salts,vitamins,and trace elements during the stage of gas recession,the activity of the methanogenic flora could be reactivated and methanogenesis concentration could be increased.The results show that enrichment and domestication of methanogenic bacteria in Longquan coal seam can significantly improve its ability to degrade bituminous coal to produce methane; supplementing nutrients during the decline of fermentation gas production can activate methanogenic bacteria and restore its ability to degrade bituminous coal to produce methane.In the recession period of pilot fermentation gas production,yeast powder,nitrogen source and phosphorus salt were added to the fermenter.Magnesium salts,vitamins and trace elements can reactivate the activity of the methanogenic flora and make it reduce the concentration of methane produced by bituminous coal.
In order to explore the potential of methanogenic bacteria in Longquan coalbed methane field to degrade methane production in bituminous coal,the enrichment and domestication of methanogens in Longquan coalbed methane field was studied.The laboratory study as well as pilot study on the degradation of coal gas production by methanogens was carried out with Longquan mine bituminous coal as parent material.The results showed that the concentration of methanogenic degradation of bituminous coal increased from 8.2% to 19% after enrichment and domestication of methanogens,the cycle of gas production stability was shortened.In the pilot test,the methanogenic concentration was up to 27.06%,and methane production rate was 0.159 m L/( g·d). By supplementing yeast powder,nitrogen sources,phosphates,magnesium salts,vitamins,and trace elements during the stage of gas recession,the activity of the methanogenic flora could be reactivated and methanogenesis concentration could be increased.The results show that enrichment and domestication of methanogenic bacteria in Longquan coal seam can significantly improve its ability to degrade bituminous coal to produce methane; supplementing nutrients during the decline of fermentation gas production can activate methanogenic bacteria and restore its ability to degrade bituminous coal to produce methane.In the recession period of pilot fermentation gas production,yeast powder,nitrogen source and phosphorus salt were added to the fermenter.Magnesium salts,vitamins and trace elements can reactivate the activity of the methanogenic flora and make it reduce the concentration of methane produced by bituminous coal.
引文
[1] WELTE C U.A microbial route from coal to gas[J].Science,2016,354:184-184.
[2]陈润,王优阳,王琳琳,等.煤层生物气组成特征及其影响因素研究进展[J].煤炭科学技术,2016,44(11):167-172.CHEN Run,WANG Youyang,WANG Linlin,et al. Research progress on characteristics of biogenic coal bed gases and its influence factors[J]. Coal Science and Technology,2016,44(11):167-172.
[3] STRAPOD,MASTALERZ M,DAWSON K,et al.Biogeochemistry of microbial coal-bed methane[J]. Annual Review of Earth and Planetary Sciences,2011,39:617-656.
[4] ROMEO M F,CYNTHA A R,GARY D S,et al. Methanogenic pathways of coal-bed gas in the Power River Basin,United States:the geologic factors[J].Int J Coal Geol,2008,76:52-75
[5] ZHENG Hang,CHEN Tianyu,VICTOR Rudolph,et al. Biogenic methane production from Bowen Basin coal waste materials[J].International Journal of Coal Geology,2017,169:22-27.
[6]郭红光,王飞,李志刚.微生物增产煤层气技术研究进展[J].微生物学报,2015,42(3):584-590.GUO Hongguang,WANG Fei,LI Zhigang.Research progress of microbially enhanced coalbed methane[J]. Microbiology China,2015,42(3):584-590.
[7] FALLGREN P H,JIN S,ZENG C,et al.Comparison of coal rank for enhanced biogenic natural gas production[J]. Int J Coal Geol,2013,115:92-96.
[8] GUO Hongguang,YU Zhisheng,ZHANG Hongxun.Phylogenetic diversity of microbial communities associated with coalbed methane gas from Eastern Ordos Basin,China[J]. International Journal of Coal Geology,2015(150/151):120-126.
[9] GUO Hongguang,YU Zhisheng,IAN P Thompson,et al.A contribution of hydrogenotrophic methanogenesis to the biogenic coal bed methane reserves of Southern Qinshui Basin,China[J]. Environmental Biotechnology,2014,98:9083-9093.
[10]李亚平,郭红光,韩青,等.水稻秸秆与煤共降解增产煤层生物甲烷试验研究[J].煤炭科学技术,2018,46(4):215-221.LI Yaping,GUO Hongguang,HAN Qing,et al. Experimental research on enhancing biogenic methane production in coal seam by co-degradation of rice straw and coal[J].Coal Science and Technology,2018,46(4):215-221.
[11]王保玉,陈林勇,邰超,等.外源菌群煤生物气化初步研究:菌群结构、煤种及煤孔(裂)隙[J].煤炭学报,2014,39(9):1797-1801.WANG Baoyu,CHEN Linyong,TAI Chao,et al. A preliminary study of biological coal gasification by exogenous bacteria:microbiome composition,coal type,pore and seam fracture[J]. Journal of China Coal Society,2014,39(9):1797-1801.
[12] LIU Jianmin,WANG Baoyu,TAI Chao,et al.An effective method to detect volatile intermediates generated in the bioconversion of coal to methane by gas chromatography-mass spectrometry after in-situextraction using headspace solid-phase micro-extraction under strict anaerobic conditions[J]. Plos One,2016,11(10):e016394910.
[13]陈林勇,王保玉,邰超,等.无烟煤微生物成气中间代谢产物组成及其转化[J].煤炭学报,2016,41(9):2305-2311CHEN Linyong,WANG Baoyu,TAI Chao,et al. Composition and conversion of intermediate products in the process of anthracite gasification by microorganism[J]. Journal of China Coal Society,2016,41(9):2305-2311.
[14] STEPHEN Y Park,LIANG Yanna.Biogenic methane production from coal:a review on recent research and development on microbially enhanced coalbed methane(MECBM)[J]. Fuel,2016,166:258-267.
[15]杨秀清,吴瑞薇,韩作颖,等.基于mcr A基因的沁水盆地煤层气田产甲烷菌群与途径分析[J].微生物学通报,2017,44(4):795-806.YANG Xiuqing,WU Ruiwei,HAN Zuoying,et al. Analysis of methanogenic community and pathway of coalbed methane fileds in the Qinshui basin based on mcr A gene[J]. Microbiology China,2017,44(4):795-806.
[16] ZHANG Ji,LIANG Yanna. Evaluating approaches for sustaining methane production from coal through biogasification[J]. Fuel,2017,102:233-240.
[17]汪涵,林海,董颖博,等.外源产甲烷菌降解褐煤产气实验[J].石油勘探与开发,2012,39(6):764-768.WANG Han,LIN Hai,DONG Yingbo,et al. Experiments on the gas production of brown coal degraded byexogenous methanogens[J]. Petroleum Exploration and Development,2012,39(6):764-768.
[18] GUO Hongguang,LIU Ruyin,YU Zhisheng,et al.Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China[J]. International Journal of Coal Geoloy,2012,93(1):56-61.
[19] UNAL B,PERRY V R,Shcth M,et al. Trace elements affect methanogenic activity and diversity in enrichments from subsurface coal bed produced water[J]. Frontiers in Microbiology,2012(3):175-180.
[20] YANG Xiuqing,CHEN Yanmei,WU Ruiwei,et al.Potential of biogenic methane for pilot-scale fermentation ex situ with lump anthracite and the changes of methanogenic consortia[J].Journal of Industrial Microbiology&Biotechnology,2018,45(4):229-237.
[21] RITTER D,VINSON D,BARNHART E,et al.Enhanced microbial coalbed methane generation:a review of research,commercial activity,and remaining challenges[J]. Int J Coal Geol,2015,146:28-41.
[22]徐鑫,梁萌.提高煤层气采收率的方法和技术进展[J].中国煤层气,2016,13(3):3-6.XU Xin,LIANG Meng. Method of improving CBM recovery rate and technologies research progress[J]. China Coalbed Methane,2016,13(3):3-6.
[23] MAYUMI D,MOCHIMARU H,TAMAKI H,et al.Methane production from coal by a single methanogen[J]. Science,2016,354:222-225.
[24] ZHANG Ji,LIANG Yanna,PANDEY R,et al.Characterizing microbial communities dedicated for conversion of coal to methane in situ[J].Int J Coal Geol,2015,146:145-154.
[25] WU Yuting,GE Xiaoguang,ZHOU Ni.Analysis on coal bed methane corresponding to groundwater chemical types in some northern China Plain coal fields[J].World Scientific,2017(2):243-250.
[26]陈浩,李贵中,陈振宏,等.微生物提高煤层气产量模拟实验研究[J].煤田地质与勘探,2016,44(4):64-68.CHEN Hao,LI Guizhong,CHEN Zhenhong,et al. Simulation experiment on enhancing coalbed methane production by microbes[J].Coal Geology&Exploration,2016,44(4):64-68.
[27]林海,隋梦琪,汪涵.微生物增产煤层气菌种的驯化[J].煤炭学报,2012,37(8):1359-1363.LIN Hai,SUI Mengqi,WANG Han. Domestication of microbially enhanced coalbed methane microorganism[J]. Journal of China Coal Society,2012,37(8):1359-1363.
[28] THAKER N,KHARDEAVIS A,PUROHIT H J.Recent advances in factors and method for stimulation of biomethane production[J].Recent Advances in Dna&Gene Sequences,2015,9(1):3-13.
[29] RITTER D,VINSON D,BARNHART E,et al.Enhanced microbial coaled methane generation:a review of research,commercial activity,and remaining challenges[J].Int J Coal Geol,2015,146:28-41.
[30] FAKOUSSA R,HOFRICHTER M.Biotechnology and microbiology of coal degradation[J].Appl Microbiol Biotechnol,1999,52:25-40.
[31] HARRIS S H,SMITH R L,BARKER C E.Microbial and chemical factors influencing methane production in laboratory incubations of low-rank subsurface coals[j]. Int J Coal Geol,2008,76:46-51.
[32] ZHANG Ji,PARK S Y,LIANG Y,et al. Finding cost-effective nutrient solutions and evaluating mental conditions for biogasifying bituminous coal to methane in situ[J].Appl Energy,2016,165:559-568.
[33] ROHIT P,SATYA H,FENG R M,et al.Changes in gas storage and transport properties of coal as a result of enhanced microbial methane generation[J].Fuel,2016,179:114-123.
[34] JONE E P J,VOYTEK M A,CORUM M D,et al.Stimulation of methane generation from nonproductive coal by addition of nutrients or a microbial consortium[J]. Applied and Environmental Microbiology,2010,76(21):7013-7022.
[35]吴鹏,魏国琴,韩作颖,等.沁水盆地寺河地区煤层水细菌多样性分析[J].生物技术,2017,27(2):161-166.WU Peng,WEI Guoqin,HAN Zuoying,et al.Bacteria diversity in coalbed water at Sihe area of Qinshui Basin[J]. Biotechnology,2017,27(2):161-166.
[36] XIAO D,PENG S P,WANG E Y.Fermentation enhancement of methanogenic archaea consortia from an illinois basin coalbed via DOL emulsion nutrition[J].Plos One,2015,10(4):124-128.
[2]陈润,王优阳,王琳琳,等.煤层生物气组成特征及其影响因素研究进展[J].煤炭科学技术,2016,44(11):167-172.CHEN Run,WANG Youyang,WANG Linlin,et al. Research progress on characteristics of biogenic coal bed gases and its influence factors[J]. Coal Science and Technology,2016,44(11):167-172.
[3] STRAPOD,MASTALERZ M,DAWSON K,et al.Biogeochemistry of microbial coal-bed methane[J]. Annual Review of Earth and Planetary Sciences,2011,39:617-656.
[4] ROMEO M F,CYNTHA A R,GARY D S,et al. Methanogenic pathways of coal-bed gas in the Power River Basin,United States:the geologic factors[J].Int J Coal Geol,2008,76:52-75
[5] ZHENG Hang,CHEN Tianyu,VICTOR Rudolph,et al. Biogenic methane production from Bowen Basin coal waste materials[J].International Journal of Coal Geology,2017,169:22-27.
[6]郭红光,王飞,李志刚.微生物增产煤层气技术研究进展[J].微生物学报,2015,42(3):584-590.GUO Hongguang,WANG Fei,LI Zhigang.Research progress of microbially enhanced coalbed methane[J]. Microbiology China,2015,42(3):584-590.
[7] FALLGREN P H,JIN S,ZENG C,et al.Comparison of coal rank for enhanced biogenic natural gas production[J]. Int J Coal Geol,2013,115:92-96.
[8] GUO Hongguang,YU Zhisheng,ZHANG Hongxun.Phylogenetic diversity of microbial communities associated with coalbed methane gas from Eastern Ordos Basin,China[J]. International Journal of Coal Geology,2015(150/151):120-126.
[9] GUO Hongguang,YU Zhisheng,IAN P Thompson,et al.A contribution of hydrogenotrophic methanogenesis to the biogenic coal bed methane reserves of Southern Qinshui Basin,China[J]. Environmental Biotechnology,2014,98:9083-9093.
[10]李亚平,郭红光,韩青,等.水稻秸秆与煤共降解增产煤层生物甲烷试验研究[J].煤炭科学技术,2018,46(4):215-221.LI Yaping,GUO Hongguang,HAN Qing,et al. Experimental research on enhancing biogenic methane production in coal seam by co-degradation of rice straw and coal[J].Coal Science and Technology,2018,46(4):215-221.
[11]王保玉,陈林勇,邰超,等.外源菌群煤生物气化初步研究:菌群结构、煤种及煤孔(裂)隙[J].煤炭学报,2014,39(9):1797-1801.WANG Baoyu,CHEN Linyong,TAI Chao,et al. A preliminary study of biological coal gasification by exogenous bacteria:microbiome composition,coal type,pore and seam fracture[J]. Journal of China Coal Society,2014,39(9):1797-1801.
[12] LIU Jianmin,WANG Baoyu,TAI Chao,et al.An effective method to detect volatile intermediates generated in the bioconversion of coal to methane by gas chromatography-mass spectrometry after in-situextraction using headspace solid-phase micro-extraction under strict anaerobic conditions[J]. Plos One,2016,11(10):e016394910.
[13]陈林勇,王保玉,邰超,等.无烟煤微生物成气中间代谢产物组成及其转化[J].煤炭学报,2016,41(9):2305-2311CHEN Linyong,WANG Baoyu,TAI Chao,et al. Composition and conversion of intermediate products in the process of anthracite gasification by microorganism[J]. Journal of China Coal Society,2016,41(9):2305-2311.
[14] STEPHEN Y Park,LIANG Yanna.Biogenic methane production from coal:a review on recent research and development on microbially enhanced coalbed methane(MECBM)[J]. Fuel,2016,166:258-267.
[15]杨秀清,吴瑞薇,韩作颖,等.基于mcr A基因的沁水盆地煤层气田产甲烷菌群与途径分析[J].微生物学通报,2017,44(4):795-806.YANG Xiuqing,WU Ruiwei,HAN Zuoying,et al. Analysis of methanogenic community and pathway of coalbed methane fileds in the Qinshui basin based on mcr A gene[J]. Microbiology China,2017,44(4):795-806.
[16] ZHANG Ji,LIANG Yanna. Evaluating approaches for sustaining methane production from coal through biogasification[J]. Fuel,2017,102:233-240.
[17]汪涵,林海,董颖博,等.外源产甲烷菌降解褐煤产气实验[J].石油勘探与开发,2012,39(6):764-768.WANG Han,LIN Hai,DONG Yingbo,et al. Experiments on the gas production of brown coal degraded byexogenous methanogens[J]. Petroleum Exploration and Development,2012,39(6):764-768.
[18] GUO Hongguang,LIU Ruyin,YU Zhisheng,et al.Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China[J]. International Journal of Coal Geoloy,2012,93(1):56-61.
[19] UNAL B,PERRY V R,Shcth M,et al. Trace elements affect methanogenic activity and diversity in enrichments from subsurface coal bed produced water[J]. Frontiers in Microbiology,2012(3):175-180.
[20] YANG Xiuqing,CHEN Yanmei,WU Ruiwei,et al.Potential of biogenic methane for pilot-scale fermentation ex situ with lump anthracite and the changes of methanogenic consortia[J].Journal of Industrial Microbiology&Biotechnology,2018,45(4):229-237.
[21] RITTER D,VINSON D,BARNHART E,et al.Enhanced microbial coalbed methane generation:a review of research,commercial activity,and remaining challenges[J]. Int J Coal Geol,2015,146:28-41.
[22]徐鑫,梁萌.提高煤层气采收率的方法和技术进展[J].中国煤层气,2016,13(3):3-6.XU Xin,LIANG Meng. Method of improving CBM recovery rate and technologies research progress[J]. China Coalbed Methane,2016,13(3):3-6.
[23] MAYUMI D,MOCHIMARU H,TAMAKI H,et al.Methane production from coal by a single methanogen[J]. Science,2016,354:222-225.
[24] ZHANG Ji,LIANG Yanna,PANDEY R,et al.Characterizing microbial communities dedicated for conversion of coal to methane in situ[J].Int J Coal Geol,2015,146:145-154.
[25] WU Yuting,GE Xiaoguang,ZHOU Ni.Analysis on coal bed methane corresponding to groundwater chemical types in some northern China Plain coal fields[J].World Scientific,2017(2):243-250.
[26]陈浩,李贵中,陈振宏,等.微生物提高煤层气产量模拟实验研究[J].煤田地质与勘探,2016,44(4):64-68.CHEN Hao,LI Guizhong,CHEN Zhenhong,et al. Simulation experiment on enhancing coalbed methane production by microbes[J].Coal Geology&Exploration,2016,44(4):64-68.
[27]林海,隋梦琪,汪涵.微生物增产煤层气菌种的驯化[J].煤炭学报,2012,37(8):1359-1363.LIN Hai,SUI Mengqi,WANG Han. Domestication of microbially enhanced coalbed methane microorganism[J]. Journal of China Coal Society,2012,37(8):1359-1363.
[28] THAKER N,KHARDEAVIS A,PUROHIT H J.Recent advances in factors and method for stimulation of biomethane production[J].Recent Advances in Dna&Gene Sequences,2015,9(1):3-13.
[29] RITTER D,VINSON D,BARNHART E,et al.Enhanced microbial coaled methane generation:a review of research,commercial activity,and remaining challenges[J].Int J Coal Geol,2015,146:28-41.
[30] FAKOUSSA R,HOFRICHTER M.Biotechnology and microbiology of coal degradation[J].Appl Microbiol Biotechnol,1999,52:25-40.
[31] HARRIS S H,SMITH R L,BARKER C E.Microbial and chemical factors influencing methane production in laboratory incubations of low-rank subsurface coals[j]. Int J Coal Geol,2008,76:46-51.
[32] ZHANG Ji,PARK S Y,LIANG Y,et al. Finding cost-effective nutrient solutions and evaluating mental conditions for biogasifying bituminous coal to methane in situ[J].Appl Energy,2016,165:559-568.
[33] ROHIT P,SATYA H,FENG R M,et al.Changes in gas storage and transport properties of coal as a result of enhanced microbial methane generation[J].Fuel,2016,179:114-123.
[34] JONE E P J,VOYTEK M A,CORUM M D,et al.Stimulation of methane generation from nonproductive coal by addition of nutrients or a microbial consortium[J]. Applied and Environmental Microbiology,2010,76(21):7013-7022.
[35]吴鹏,魏国琴,韩作颖,等.沁水盆地寺河地区煤层水细菌多样性分析[J].生物技术,2017,27(2):161-166.WU Peng,WEI Guoqin,HAN Zuoying,et al.Bacteria diversity in coalbed water at Sihe area of Qinshui Basin[J]. Biotechnology,2017,27(2):161-166.
[36] XIAO D,PENG S P,WANG E Y.Fermentation enhancement of methanogenic archaea consortia from an illinois basin coalbed via DOL emulsion nutrition[J].Plos One,2015,10(4):124-128.
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