血清miR-182与CA153联合检测在胆囊癌诊断中的意义
|
摘要
目的探究胆囊癌患者血清中miR-182与糖类抗原153(CA153)的表达,分析二者在胆囊癌诊断中的临床价值。方法选取72例胆囊癌以及47例胆囊炎患者血液样本,采用荧光定量PCR法(QPCR)检测miR-182表达,酶联免疫法检测CA153水平,分析二者与患者临床病理参数的关系,受试者工作特征(ROC)曲线评估miR-182诊断胆囊癌最佳的临界值;对比miR-182与CA153联合检测以及单项检测在胆囊癌诊断中的诊断效能。结果与胆囊炎组相比,胆囊癌组患者血清miR-182相对表达量、CA153水平均升高,差异有统计学意义(P<0.05)。miR-182、CA153表达与患者性别、年龄、病理类型、肿瘤分化程度无关(P>0.05),而与淋巴结转移、TNM分期有关(P<0.05)。ROC曲线分析显示miR-182截断值为2.03,灵敏度为70.83%,特异度为76.60%;CA153诊断灵敏度为56.94%,特异度为68.09%,准确度为61.34%。整体检测以及在淋巴结转移、TNM不同分期中,miR-182联合CA153诊断灵敏度、准确度均高于单项检测,差异有统计学意义(P<0.05)。结论 miR-182与CA153在胆囊癌患者血清中均表达上调,与肿瘤分期、淋巴结转移有关,二者联合检测诊断效能较高,可为胆囊癌患者临床诊治提供参考。
Objective To investigate the expressions of miR-182 and carbohydrate antigen 153(CA153) in serum of patients with gallbladder carcinoma and analyze their clinical value for the diagnosis of gallbladder carcinoma. Methods The blood samples of 72 patients with gallbladder cancer and 47 patients with cholecystitis were collected. The expression of miR-182 was detected by fluorescence quantitative PCR(QPCR);enzyme-linked immunosorbent assay(ELISA)was used to detect CA153 level;the relationships between the two parameters with clinical pathological parameters were analyzed;the receiver operating characteristic(ROC)curve was used to evaluate the best critical value of miR-182 in diagnosing gallbladder cancer. Results Compared with cholecystitis group,the relative expression level of serum miR-182 and CA153 level in gallbladder carcinoma group increased;the differences were statistically significant(P<0.05). The expressions of miR-182 and CA153 were not related to gender,age,pathological type and tumor differentiation degree(P>0.05);it was related to lymph node metastasis and TNM staging;the differences were statistically significant(P<0.05). ROC curve analysis showed that the cut-off value of miR-182 was 2.03,the sensitivity was 70.83%,and the specificity was 76.60%;the sensitivity of CA153 was 56.94%,the specificity was 68.09%,and the accuracy was 61.34%. In overall detection,the lymph node metastasis and different stages of TNM,the sensitivity and accuracy of miR-182 and CA153 joint detection were higher than those of the single detection,and the differences were statistically significant(P<0.05). Conclusion MiR-182 and CA153 were up-regulated in serum of patients with carcinoma of the gallbladder;it was related to tumor staging and lymph node metastasis;the joint detection of the two was highly effective for diagnosis and might be the targets for treating carcinoma of the gallbladder.
Objective To investigate the expressions of miR-182 and carbohydrate antigen 153(CA153) in serum of patients with gallbladder carcinoma and analyze their clinical value for the diagnosis of gallbladder carcinoma. Methods The blood samples of 72 patients with gallbladder cancer and 47 patients with cholecystitis were collected. The expression of miR-182 was detected by fluorescence quantitative PCR(QPCR);enzyme-linked immunosorbent assay(ELISA)was used to detect CA153 level;the relationships between the two parameters with clinical pathological parameters were analyzed;the receiver operating characteristic(ROC)curve was used to evaluate the best critical value of miR-182 in diagnosing gallbladder cancer. Results Compared with cholecystitis group,the relative expression level of serum miR-182 and CA153 level in gallbladder carcinoma group increased;the differences were statistically significant(P<0.05). The expressions of miR-182 and CA153 were not related to gender,age,pathological type and tumor differentiation degree(P>0.05);it was related to lymph node metastasis and TNM staging;the differences were statistically significant(P<0.05). ROC curve analysis showed that the cut-off value of miR-182 was 2.03,the sensitivity was 70.83%,and the specificity was 76.60%;the sensitivity of CA153 was 56.94%,the specificity was 68.09%,and the accuracy was 61.34%. In overall detection,the lymph node metastasis and different stages of TNM,the sensitivity and accuracy of miR-182 and CA153 joint detection were higher than those of the single detection,and the differences were statistically significant(P<0.05). Conclusion MiR-182 and CA153 were up-regulated in serum of patients with carcinoma of the gallbladder;it was related to tumor staging and lymph node metastasis;the joint detection of the two was highly effective for diagnosis and might be the targets for treating carcinoma of the gallbladder.
引文
[1]耿智敏,王林,陈晨,等.438例胆囊癌外科治疗及预后因素分析[J].中华消化外科杂志,2016,15(4):346-352.
[2]林德,王立明.miR-31与miR-182在结直肠癌中表达及临床意义[J].世界华人消化杂志,2018,26(14):135-138.
[3]马列.miR-182表达异常对甲状腺癌细胞株SW579增殖迁移的影响[J].现代肿瘤医学,2016,24(5):699-702.
[4]刘新唐,李玉柱,韩龙才,等.血清CA153、TPS、CYFRA21-1检测联合钼靶X摄影在乳腺癌早期诊断中的价值[J].中国热带医学,2018,18(2):150-153.
[5]Mooi WJ.UICC TNM atlas:illustrated guide to the TNM/pTNMclassification of malignant tumours(4th edition)[J].J Clin Pathol,1999,52(3):239.
[6]殷保兵,王一飞,杨光华,等.miR-135a-5p抑制胆囊癌细胞增殖的研究[J].外科理论与实践,2014,19(2):140-144.
[7]邱伟,杨成琳,刘阳.miR-210通过下调JAK-STAT信号通路抑制胆囊癌细胞增殖的实验研究[J].陕西医学杂志,2016,45(6):652-655.
[8]鲁之中,李军民,胡云芝,等.食管癌患者miR-182表达水平及其对预后的影响[J].中国现代医学杂志,2017,27(24):39-43.
[9]刘云,甘为,张正龙,等.miR-182在前列腺癌组织中的表达及其对前列腺癌细胞增殖和凋亡的影响[J].现代生物医学进展,2018,18(9):126-129.
[10]张艳艳,徐爱晖,李永怀,等.血浆microRNA-182在非小细胞肺癌中的表达及临床意义[J].安徽医科大学学报,2016,51(4):583-586.
[11]王丽纳,王凯波,庄永灿,等.miR-182靶向作用环磷酸腺苷反应元件结合蛋白1调控人黑素瘤A375细胞的增殖和侵袭[J].解剖学报,2017,48(6):710-714.
[12]邱应和.TGF-β上调miR-182表达抑制靶向CADM1促进胆囊癌转移的研究[D].上海:第二军医大学,2014.
[13]刘广阔,侯志平,何培元,等.Hsa-miR-182-5p在结直肠癌组织中表达的临床意义及预后分析[J].世界最新医学信息文摘,2018,18(34):113-116.
[14]李梅.血清CEA、CA153、NSE、CYFRA21-1联合检测在肺癌诊断中的应用[J].山东医药,2014,54(22):53-55.
[15]李娜,李宏斌,朱蕾,等.血清CEA、CA199和CA153检测对判定结肠癌发生发展的价值分析[J].实用癌症杂志,2016,31(3):396-398.
[16]梁嘉晖,谢淑华.CEA、CA125和CA153与乳腺癌病理特征的关系及其术后化疗动态监测的意义[J].现代医院,2015,15(12):53-55.2018-10-04
[2]林德,王立明.miR-31与miR-182在结直肠癌中表达及临床意义[J].世界华人消化杂志,2018,26(14):135-138.
[3]马列.miR-182表达异常对甲状腺癌细胞株SW579增殖迁移的影响[J].现代肿瘤医学,2016,24(5):699-702.
[4]刘新唐,李玉柱,韩龙才,等.血清CA153、TPS、CYFRA21-1检测联合钼靶X摄影在乳腺癌早期诊断中的价值[J].中国热带医学,2018,18(2):150-153.
[5]Mooi WJ.UICC TNM atlas:illustrated guide to the TNM/pTNMclassification of malignant tumours(4th edition)[J].J Clin Pathol,1999,52(3):239.
[6]殷保兵,王一飞,杨光华,等.miR-135a-5p抑制胆囊癌细胞增殖的研究[J].外科理论与实践,2014,19(2):140-144.
[7]邱伟,杨成琳,刘阳.miR-210通过下调JAK-STAT信号通路抑制胆囊癌细胞增殖的实验研究[J].陕西医学杂志,2016,45(6):652-655.
[8]鲁之中,李军民,胡云芝,等.食管癌患者miR-182表达水平及其对预后的影响[J].中国现代医学杂志,2017,27(24):39-43.
[9]刘云,甘为,张正龙,等.miR-182在前列腺癌组织中的表达及其对前列腺癌细胞增殖和凋亡的影响[J].现代生物医学进展,2018,18(9):126-129.
[10]张艳艳,徐爱晖,李永怀,等.血浆microRNA-182在非小细胞肺癌中的表达及临床意义[J].安徽医科大学学报,2016,51(4):583-586.
[11]王丽纳,王凯波,庄永灿,等.miR-182靶向作用环磷酸腺苷反应元件结合蛋白1调控人黑素瘤A375细胞的增殖和侵袭[J].解剖学报,2017,48(6):710-714.
[12]邱应和.TGF-β上调miR-182表达抑制靶向CADM1促进胆囊癌转移的研究[D].上海:第二军医大学,2014.
[13]刘广阔,侯志平,何培元,等.Hsa-miR-182-5p在结直肠癌组织中表达的临床意义及预后分析[J].世界最新医学信息文摘,2018,18(34):113-116.
[14]李梅.血清CEA、CA153、NSE、CYFRA21-1联合检测在肺癌诊断中的应用[J].山东医药,2014,54(22):53-55.
[15]李娜,李宏斌,朱蕾,等.血清CEA、CA199和CA153检测对判定结肠癌发生发展的价值分析[J].实用癌症杂志,2016,31(3):396-398.
[16]梁嘉晖,谢淑华.CEA、CA125和CA153与乳腺癌病理特征的关系及其术后化疗动态监测的意义[J].现代医院,2015,15(12):53-55.2018-10-04