母猪饲粮中添加过氧化氢酶对母猪及仔猪生长性能和抗氧化能力的影响
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摘要
本试验旨在研究母猪妊娠后期和哺乳期饲粮中添加过氧化氢酶(CAT)对母猪繁殖性能、抗氧化能力、饲粮养分消化率以及仔猪生长性能和抗氧化能力的影响。试验选取二元杂交(长白×大白)经产母猪160头,按母猪预产期、胎次体重、体况相近均衡分布的原则随机分为4组(对照组和试验A、B、C组),饲粮中CAT添加量分别为0、50、100、150 U/kg,每组40头,每头母猪为1个重复,试验周期从妊娠第90天开始至仔猪断奶。结果表明:与对照组相比,饲粮中添加CAT对总产仔头数、活仔数、健仔数、初生窝重有提高但未达到显著水平(P>0.05);在母猪饲粮中添加CAT对降低哺乳仔猪腹泻率效果显著(P<0.05);在母猪妊娠第104天,与对照组相比,试验B和C组中血清总抗氧化能力(T-AOC)分别提高60.03%和53.70%(P<0.05);试验B组中血清CAT活性较对照组、试验A和C组都有显著提高(P <0.05)。在母猪哺乳第14天,与对照组相比,试验B组中血清CAT活性显著提高(P<0.05);与对照组相比,试验B组初乳中T-AOC提高119.10%(P<0.05);试验B和C组的初乳CAT活性较对照组分别提高86.24%和76.95%(P<0.05);各试验组在粗蛋白质消化率上与对照组相比差异均为显著(P <0. 05);各试验组在粗脂肪消化率上与对照组相比差异均为显著(P<0.05)。综上,在母猪饲粮中添加CAT可以一定程度上提高母猪总产仔数、母猪妊娠第104天及哺乳第14天血清中T-AOC、CAT活性、初乳中T-AOC、CAT活性,母猪抗氧化能力在饲粮中添加100 U/kg CAT时提升最明显;在母猪饲粮中添加CAT可以显著提高粗脂肪和粗蛋白质消化率。
This experiment aimed to investigate the effects of catalase( CAT) supplementation in diets of sows during late gestation and lactation on reproductive performance,antioxidant capacity and digestibility of sows and piglets' growth performance and antioxidant capacity. In experiment,a total of 160 healthy crossbred sows( Landrace×Yorkshire) were used and randomly divided into 4 groups( control group,test A group,test B group and test C group),with the supplementation of CAT at 0,50,100 and 150 U/kg,respectively,and each group had 40 sows with 1 sowper replicate. The experiment started from day 90 of pregnancy to weaning of piglets. The results showed that compared with the control group,the supplementation of CAT increased the number of total piglets born,number of born alive,number of born healthy and birth litter weight of piglets but did not reach the significant level( P>0.05). Adding CAT in sows' diets had a significant effect on decreasing the diarrhea rates of piglets( P<0.05); at day 104 of pregnancy,compared with the control group,the serum total antioxidant capacity( T-AOC) in test groups B and C increased by 60.03% and 53.70%( P<0.05),respectively. The activity of CAT in serum in test B group was higher than that in control group,test groups A and C( P<0.05). At day 14 of lactation,compared with the control group,the activity of CAT in serum in test B group significantly increased( P<0.05); compared with the control group,T-AOC in colostrums in test C group increased by 119.10%( P<0.05). Compared with control group A,the activity of CAT in colostrums in test groups B and C increased by 86.24% and 76.95%( P<0.05); all test groups had significant differences in the crude fat and ether extract digestibility compared with control group( P < 0. 05). The results showthat adding CAT in the diet of pregnant sowcan improve the number of total birth piglets,T-AOC,GSH content and CAT activity of serum at day 104 of pregnancy and day 14 of lactation,and the T-AOC and CAT activity in colostrum; the sowantioxidant capacity improves the most when 100 U/kg CAT added to the sowdiets;adding CAT in the sowdiets can significantly improve the digestibility of crude fat and ether extract.[Chinese Journal of Animal Nutrition,2019,31(7):3268-3275]
This experiment aimed to investigate the effects of catalase( CAT) supplementation in diets of sows during late gestation and lactation on reproductive performance,antioxidant capacity and digestibility of sows and piglets' growth performance and antioxidant capacity. In experiment,a total of 160 healthy crossbred sows( Landrace×Yorkshire) were used and randomly divided into 4 groups( control group,test A group,test B group and test C group),with the supplementation of CAT at 0,50,100 and 150 U/kg,respectively,and each group had 40 sows with 1 sowper replicate. The experiment started from day 90 of pregnancy to weaning of piglets. The results showed that compared with the control group,the supplementation of CAT increased the number of total piglets born,number of born alive,number of born healthy and birth litter weight of piglets but did not reach the significant level( P>0.05). Adding CAT in sows' diets had a significant effect on decreasing the diarrhea rates of piglets( P<0.05); at day 104 of pregnancy,compared with the control group,the serum total antioxidant capacity( T-AOC) in test groups B and C increased by 60.03% and 53.70%( P<0.05),respectively. The activity of CAT in serum in test B group was higher than that in control group,test groups A and C( P<0.05). At day 14 of lactation,compared with the control group,the activity of CAT in serum in test B group significantly increased( P<0.05); compared with the control group,T-AOC in colostrums in test C group increased by 119.10%( P<0.05). Compared with control group A,the activity of CAT in colostrums in test groups B and C increased by 86.24% and 76.95%( P<0.05); all test groups had significant differences in the crude fat and ether extract digestibility compared with control group( P < 0. 05). The results showthat adding CAT in the diet of pregnant sowcan improve the number of total birth piglets,T-AOC,GSH content and CAT activity of serum at day 104 of pregnancy and day 14 of lactation,and the T-AOC and CAT activity in colostrum; the sowantioxidant capacity improves the most when 100 U/kg CAT added to the sowdiets;adding CAT in the sowdiets can significantly improve the digestibility of crude fat and ether extract.[Chinese Journal of Animal Nutrition,2019,31(7):3268-3275]
引文
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[13]冯俊,邓斐月,余燕玲,等.微囊化β-胡萝卜素对妊娠母猪繁殖、免疫和抗氧化功能的影响[J].浙江大学学报(农业与生命科学版),2009,35(6):665-669.
[14]李青萍,乔秀红,王向东.饲粮中添加维生素E对猪肉质的影响[J].中国畜牧杂志,2003,39(05):34-35.
[15] OWENS C M,SAMS A R.The influence of transportation on turkey meat quality[J]. Poultry Science,2000,79(8):1204-1207.
[16]陈伟,李华,曾勇庆,等.饲粮添加大蒜对肥育猪肌肉抗氧化性能及肉质特性的影响[J].养猪,2010(2):30-32.
[17]郭小权,胡国良,曹华斌,等.不同形式和水平的锌对断奶仔猪抗氧化功能的影响[J].黑龙江畜牧兽医,2010(9):64-65.
[18]范文娜,何瑞国.维生素E对溆浦鹅肥肝抗氧化性能和血液生化指标影响的研究[J].中国粮油学报,2008,23(3):124-130.
[19] JU R T,WEI H P,WANG F,et al.Anaerobic respiration and antioxidant responses of Corythucha ciliata(Say)adults to heat-induced oxidative stress under laboratory and field conditions[J]. Cell Stress and Chaperones,2014,19(2):255-262.
[2] HERSCU-KLUSKA R,MASARWA A,SAPHIER M,et al. Mechanism of the reaction of radicals with peroxides and dimethyl sulfoxide in aqueous solution[J].Chemistry,2008,14(19):5880-5889.
[3]张连琴,陈雪娴.自由基与疾病[J].天津医科大学学报,1997,3(2):85-88.
[4]孙婷婷.微生物源性抗氧化剂对动物繁殖性能和自由基代谢的影响[D].硕士学位论文.上海:上海交通大学,2007.
[5]孙婷婷,徐建雄.微生物源性抗氧化剂对母猪繁殖性能和自由基代谢的影响[J].上海交通大学学报(农业科学版),2007(4):342-346.
[6] VZQUEZ-AN M,JENKINS T.Effects of feeding oxidized fat with or without dietary antioxidants on nutrient digestibility,microbial nitrogen,and fatty acid metabolism[J]. Journal of Dairy Science,2007,90(9):4361-4367.
[7]何惠聪.哺乳仔猪生理特点与腹泻的防治[J].福建畜牧兽医,2007,29(1):38-39.
[8]李永义,段绪东,赵娇,等.茶多酚对氧化应激仔猪生长性能和免疫功能的影响[J].中国畜牧杂志,2011,47(15):53-57.
[9]杜会堂,马文强,杨晓静,等.日粮中添加茴香和大蒜素对母猪初乳抗氧化特性及仔猪早期生长性能的影响[J].畜牧与兽医,2012,44(12):17-19.
[10]岳敏,许丽,方翠林,等.丁酸钠对母猪生产性能和抗氧化功能及后代仔猪生长的影响[J].中国畜牧杂志,2014,50(23):44-47.
[11]张金枝.日粮能量结构对母猪繁殖和泌乳性能的影响研究[D].博士学位论文.杭州:浙江大学,2009.
[12] JIN L H,BAHN J H,EUMW S,et al.Transduction of human catalase mediated by an HIV-1 TAT protein basic domain and arginine-rich peptides into mammalian cells[J]. Free Radical Biology and Medicine,2001,31(11):1509-1519.
[13]冯俊,邓斐月,余燕玲,等.微囊化β-胡萝卜素对妊娠母猪繁殖、免疫和抗氧化功能的影响[J].浙江大学学报(农业与生命科学版),2009,35(6):665-669.
[14]李青萍,乔秀红,王向东.饲粮中添加维生素E对猪肉质的影响[J].中国畜牧杂志,2003,39(05):34-35.
[15] OWENS C M,SAMS A R.The influence of transportation on turkey meat quality[J]. Poultry Science,2000,79(8):1204-1207.
[16]陈伟,李华,曾勇庆,等.饲粮添加大蒜对肥育猪肌肉抗氧化性能及肉质特性的影响[J].养猪,2010(2):30-32.
[17]郭小权,胡国良,曹华斌,等.不同形式和水平的锌对断奶仔猪抗氧化功能的影响[J].黑龙江畜牧兽医,2010(9):64-65.
[18]范文娜,何瑞国.维生素E对溆浦鹅肥肝抗氧化性能和血液生化指标影响的研究[J].中国粮油学报,2008,23(3):124-130.
[19] JU R T,WEI H P,WANG F,et al.Anaerobic respiration and antioxidant responses of Corythucha ciliata(Say)adults to heat-induced oxidative stress under laboratory and field conditions[J]. Cell Stress and Chaperones,2014,19(2):255-262.
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