不同稀释液中添加十二烷基硫酸钠对吐鲁番黑羊精液冷冻保存的影响

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英文篇名：Effects of Different Diluents Supplement with Sodium Dodecylsulfate on Cryopreservation of Turpan Black Sheep Semen 作者：宋玉坤 ; 张博 ; 吴昊 ; 王辉 ; 郜宇 ; 努日比娅姆·买买提托合提 ; 赵茜 ; 王旭光 ; 刘国世 ; 阿布力孜·吾斯曼 英文作者：SONG Yu-kun;ZHANG Bo;WU Hao;WANG Hui;GAO Yu;Nuribiyamu·MAIMAITITUOHETI;ZHAO Xi;WANG Xu-guang;LIU Guo-shi;Abulizi·WUSIMAN;College of Animal Science, Xinjiang Agricultural University;College of Animal Science And Technology, China Agricultural University;Huishang Ecological Animal Husbandry Co., Ltd.; 关键词：吐鲁番黑羊 ; 十二烷基硫酸钠(SDS) ; 冷冻稀释液 ; 精液冷冻保存 ; 精子分析系统(CASA) 英文关键词：Turpan black sheep;;Sodium dodecylsulfate;;Frozen dilution;;Semen cryopreservation;;Computer-aided sperm analysis 中文刊名：ZGXM 英文刊名：Chinese Journal of Animal Science 机构：新疆农业大学动物科学学院;中国农业大学动物科学技术学院;徽商生态牧业有限公司; 出版日期：2019-07-10 出版单位：中国畜牧杂志 年：2019 期：v.55 基金：新疆维吾尔自治区科技支疆项目(2016E02037) 语种：中文; 页：ZGXM201907005 页数：5 CN：07 ISSN：11-2083/S 分类号：26-30

摘要

为改善吐鲁番黑羊精液的冷冻保存效果,提高精液冻后的精子活率,本实验在3种精液冷冻稀释液中添加不同浓度(0、1%、2%)的十二烷基硫酸钠(SDS),Tris-葡萄糖-柠檬酸钠为I液,蔗糖-葡糖糖-柠檬酸钠为II液,Optidyl稀释液做对照为III液。熏蒸冷冻后解冻,用精子分析系统(CASA)和流式细胞仪检测冷冻效果。结果表明:I液1%SDS组精子活率最高(65.4%),与I液2%SDS组精子活率没有差异,高于其他7组(P<0.05);III液1%SDS组畸形率(9.4%)低于II液0%SDS组、II液2%SDS组和III液2%SDS组(P<0.05);I液1%SDS组直线运动速率(51.4μm/s)高于I液0%SDS组、II液2%SDS组和III液0%SDS组(P<0.05);I液1%SDS组和III液1%SDS组的质膜完整率高于I液2%SDS组和II液1%SDS组(P<0.05),且I液1%SDS组最高;I液1%SDS组的顶体完整活精子比率高于II液1%SDS组(P<0.05),各组的活精子顶体完整率无显著差异;I液1%SDS组高线粒体膜电位比率(43.0%)高于II液1%SDS组(P<0.05)。因此,采用添加1%SDS的Tris-葡萄糖-柠檬酸钠稀释液配方显著提高了吐鲁番黑羊精液冷冻保存效果。
        In order to improve the cryopreservation effect of the Turpan black sheep sperm and improve sperm motility after semen freezing, in this experiment, different concentrations(0, 1%, 2%) of sodium dodecyl sulfate(SDS), were added to the three sperm frozen dilutions. Tris-Glucose-sodium citrate is liquid I, sucrose-glucose-sodium citrate is liquid II, and Optidyl diluent is used as a control liquid. After fumigation and freezing, they were thawed, and the sperm analysis system(CASA) and flow cytometry were used to detect the freezing effect. The results showed that the sperm viability rate of the 1% SDS group in liquid I was the highest(65.4%), with no difference from that in the 2% SDS group of fluid I, and significantly higher than that in the 7 groups(P<0.05); the Deformity rate of the 1% SDS group in liquid III was the lowest(9.4%), significantly lower than II liquid 0% SDS group, II liquid 2% SDS group and III liquid 2% SDS group(P<0.05); the linear motion rate of the 1% SDS group(51.4 μm/s) in liquid I was the highest, significantly higher than I solution 0% SDS group, II liquid 2% SDS group and III liquid 0% SDS group(P<0.05). The plasma membrane integrity rate of the I liquid 1% SDS group and the III liquid 1% SDS group was significantly higher than that of the I liquid 2% SDS group and the II liquid 1% SDS group(P<0.05), and the I liquid 1% SDS group was the highest; The proportion of acrosome intact live sperm in the I liquid 1% SDS group was significantly higher than that in the II liquid 1% SDS group(P<0.05). The acrosome integrity rate of live sperm in each group was not significant difference; The proportion of high mitochondrial membrane potential in the I liquid 1% SDS group was the highest(43.0%), which was significantly higher than that in the II liquid 1% SDS group(P<0.05). Therefore, significantly improved Turpan Black Sheep semen cryopreservation result by using a Tris-glucose-sodium citrate dilution formulation with 1% SDS.

引文

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