外源施加多胺对提高玉米种子耐冷性的研究
摘要
以玉米耐冷自交系辽2386和冷敏感自交系辽6082为材料,研究了以外源施加精胺(Spm)和亚精胺(Spd)作为引发剂,对玉米种子吸胀中的耐冷性、发芽能力和苗期生物量的影响,探讨利用外源多胺缓解玉米低温胁迫的相关机制。结果表明,种子经Spd和Spm引发低温(5℃)吸胀后,辽6082发芽率为93%,和引发前相比显著提高了12%,且苗高显著提高了44.62%,苗鲜重显著增加了26.76%。同时,Spd和Spm引发提高了低温胁迫期间两自交系胚中超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性,降低了丙二醛(MDA)的含量。自交系辽2386在种子吸胀期间的耐冷性强于辽6082,而不同自交系、不同器官对多胺敏感性不同。总之,Spd和Spm处理能提高玉米种子吸胀期间的耐冷性,提高低温胁迫下种子的抗氧化能力和发芽能力。
引文
[1]王琪,马树庆,郭建平,张铁林,于海,徐丽萍.温度对玉米生长和产量的影响.生态学杂志,2009,28(2):255-260
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[7]Kamiab F,Talaie A,Masood K,Amanallah J.Exogenous application of free polyamines enhance salt toleranceof pistachio(Pistacia vera L.)seedlings.Plant Growth Regulation,2014,72(3):257-268
[8]Duan H G,Yuan S,Liu W J,Xi D H,Qing D H,Liang H G,Lin H H.Effects of exogenous spermidine on photosystemⅡof wheat seedlings under water stress.Journal of Integrative Plant Biology,2006,48(8):920-927
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[10]李佳,刘杨,羌维民,王棹仁,温晓霞,廖允成.镉胁迫下多胺对玉米苗期生长的影响及其机理.农业环境科学学报,2015,34(6):1021-1027
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[15]张福锁.植物营养生态生理学和遗传学.北京:中国科学技术出版社,1993
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[18]马延华,王庆祥,陈绍江.玉米耐寒性生理生化机理与分子遗传研究进展.玉米科学,2013,21(3):76-81
[19]Heydecker W.Germination of an idea:the priming of seeds.University of Nottingham School of Agriculture Report,1974:50-67
[20]阮松林,薛庆中.植物的种子引发.植物生理学通讯,2002,38(2):198-202
[21]张卫华,郝丽珍,胡宁宝,张进文.种子引发及其效应.种子,2004,23(6):49-51
[22]左冰云,解君,韩清芳,崔雯雯,刘铁宁,王兴义,丁瑞霞.赤霉素GA4+7种子引发处理对玉米光合作用及产量的影响.农药学学报,2017,19(3):331-340
[23]王莉,管博,周沫,于君宝,张晓龙,尹娜,王栋.种子引发对甜高粱和玉米种子耐盐性的影响.种子,2015,34(6):72-77
[24]杜锦,蒙雨莲,向春阳,李润枝,何丽丽.种子引发对盐胁迫下玉米种子萌发及幼苗生长的影响.天津农学院学报,2012,19(1):1-6
[25]张彦萍.种子引发提高茄子种子活力及幼苗抗冷性的效应及机理研究.保定:河北农业大学,2011
[26]Andronis E A,Moschou P N,Toumi I,Rouelakis-Angelakis K A.Peroxisomal polyamine oxidase and NADPH-oxidase cross-talk for ROS homeostasis which affects respiration rate in Arabidopsis thaliana.Frontiers in Plant Science,2014,5(2):132-142
[27]Song Y,Diao Q,Qi H.Polyamine metabolism and biosynthetic genes expression in tomato(Lycopersicon esculentum Mill.)seedlings during cold acclimation.Plant Growth Regulation,2015,75(1):21-32
[2]Lyons J M.Chilling injury in plants.Annu Rev Plant Physiol,1973,24:445-466
[3]Imai R,Ali A,Pramanik M H R,Nakaminami K,Sentoku N,Kato H.Adistinctive class of spermidine synthase is involved in chilling response in rice.Journal of Plant Physiology,2004,161(7):883-886
[4]Liang Y L,Lur H S.Conjugated and free polyamine levels in normal and aborting maize kernels.Crop Science,2002,42(4):1217-1224
[5]Liu H P,Dong B H,Zhang Y Y,Liu Z P,Liu Y L.Relationship between osmotic stress and the levels of free,conjugated and bound polyamines in leaves of wheat seedlings.Plant Science,2004,166(5):1261-1267
[6]Liu J H,Nada K,Honda C,Honda C,Kitashiba H,Wen X P,Pang X M,Moriguchi T.Polyamine biosynthesis of apple callus under salt stress:Importance of arginine decarboxylase pathway in stress response.Journal of Experimental Botany,2006,57(11):2589-2599
[7]Kamiab F,Talaie A,Masood K,Amanallah J.Exogenous application of free polyamines enhance salt toleranceof pistachio(Pistacia vera L.)seedlings.Plant Growth Regulation,2014,72(3):257-268
[8]Duan H G,Yuan S,Liu W J,Xi D H,Qing D H,Liang H G,Lin H H.Effects of exogenous spermidine on photosystemⅡof wheat seedlings under water stress.Journal of Integrative Plant Biology,2006,48(8):920-927
[9]刘冰,周新国,李彩霞,甄博,李会贞,李中阳.叶面喷施外源多胺提高夏玉米灌浆前期抗涝性.农业工程学报,2016,32(12):122-128
[10]李佳,刘杨,羌维民,王棹仁,温晓霞,廖允成.镉胁迫下多胺对玉米苗期生长的影响及其机理.农业环境科学学报,2015,34(6):1021-1027
[11]崔宝明.外源多胺对玉米愈伤组织再生植株的影响.现代农业科技,2018(6):2-4
[12]Madakadze R M,Senaratna T.Effect of growth regulators on maturation of geranium(Pelargonium×hortorum)somatic embryos.Plant Growth Regulation,2000,30(1):55-60
[13]国家技术监督局.农作物种子检验规程:发芽试验(GB/T3543.4-1995).北京:中国标准出版社,1995
[14]张蜀秋.植物生理学实验技术教程.北京:科学出版社,2011
[15]张福锁.植物营养生态生理学和遗传学.北京:中国科学技术出版社,1993
[16]李霞,李连禄,王美云,李立公,彭金凤,赵明.玉米不同基因型对低温吸胀的响应及幼苗生长分析.玉米科学,2008,16(2):60-65
[17]秦晓惠,李凯文,段志坤.植物冷胁迫信号研究进展.分子植物育种,2018,16(21):7187-7194
[18]马延华,王庆祥,陈绍江.玉米耐寒性生理生化机理与分子遗传研究进展.玉米科学,2013,21(3):76-81
[19]Heydecker W.Germination of an idea:the priming of seeds.University of Nottingham School of Agriculture Report,1974:50-67
[20]阮松林,薛庆中.植物的种子引发.植物生理学通讯,2002,38(2):198-202
[21]张卫华,郝丽珍,胡宁宝,张进文.种子引发及其效应.种子,2004,23(6):49-51
[22]左冰云,解君,韩清芳,崔雯雯,刘铁宁,王兴义,丁瑞霞.赤霉素GA4+7种子引发处理对玉米光合作用及产量的影响.农药学学报,2017,19(3):331-340
[23]王莉,管博,周沫,于君宝,张晓龙,尹娜,王栋.种子引发对甜高粱和玉米种子耐盐性的影响.种子,2015,34(6):72-77
[24]杜锦,蒙雨莲,向春阳,李润枝,何丽丽.种子引发对盐胁迫下玉米种子萌发及幼苗生长的影响.天津农学院学报,2012,19(1):1-6
[25]张彦萍.种子引发提高茄子种子活力及幼苗抗冷性的效应及机理研究.保定:河北农业大学,2011
[26]Andronis E A,Moschou P N,Toumi I,Rouelakis-Angelakis K A.Peroxisomal polyamine oxidase and NADPH-oxidase cross-talk for ROS homeostasis which affects respiration rate in Arabidopsis thaliana.Frontiers in Plant Science,2014,5(2):132-142
[27]Song Y,Diao Q,Qi H.Polyamine metabolism and biosynthetic genes expression in tomato(Lycopersicon esculentum Mill.)seedlings during cold acclimation.Plant Growth Regulation,2015,75(1):21-32