乳酸细菌转化甘蔗糖蜜为乳酸等有机酸的研究
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摘要
乳酸是重要的工业原料,乳酸、乙酸等C_2～C_5短链脂肪酸是污水高效除磷脱氮所需的碳源。已有研究表明乳酸菌可把甘蔗糖蜜的发酵糖转化成乳酸或乳酸、乙酸等的混合物。为此,试验选用6株产酸优势乳酸细菌对5种不同配比的甘蔗糖蜜进行发酵,测定了发酵过程中的产酸量,乳酸、乙酸、甲酸的含量及糖分利用率等指标。结果表明:不同糖分配比发酵液中的酸产量达100 g/L;菌株MX4和SN2可高效转化甘蔗糖蜜与葡萄糖配比为3︰1培养基中的糖分; SN2菌株更适合于用甘蔗糖蜜生产乳酸, MX4菌株的甘蔗糖蜜发酵液更适于做污水处理的碳源补给,这两株菌具有工业化应用前景。
Sugarcane molasses contains a large amount of fermentable sugars and was an excellent carbon source for microbial fermentation. Most of lactic acid bacteria could convert the reduced sugar of sugarcane molasses into lactic acid or C_2-C_5 organic acids such as lactic acid, acetic acid, etc. Lactic acid was an important industrial raw material, and C_2-C_5 organic acids were important carbon sources needed for efficient phosphorus and nitrogen removal from sewage. Five different fermentation media were configured for this experiment. In order to screen out the highly fermentable sugarcane molasses culture medium and its corresponding specific strain, six strains of lactic acid bacteria were used to perform fermentation in these media. The amount of acid produced in the fermentation broth was determined by intermittent titration. The contents of lactic acid, acetic acid, and formic acid in the fermentation broth were determined by HPLC, respectively. The rate of sugar consumption performed by each strain was determined by DNS method. The results showed that the acid production of lactic acid bacteria reached above 100 g/L in different media. Among the consumption rations of sugars performed by the six strains in different media,the highest consumption ration was obtained by the strain MX4 followed by the strain SN2 in the fermentation medium composed by the 3︰1 ratio of sugarcane molasses to glucose. Based on the results from HPLC, strain SN2 was more suitable for the production of lactic acid from sugarcane molasses, and strain MX4 was more suitable for the production of d C_2-C_5 organic acids. These two strains could be selected as potential industrial strains.
Sugarcane molasses contains a large amount of fermentable sugars and was an excellent carbon source for microbial fermentation. Most of lactic acid bacteria could convert the reduced sugar of sugarcane molasses into lactic acid or C_2-C_5 organic acids such as lactic acid, acetic acid, etc. Lactic acid was an important industrial raw material, and C_2-C_5 organic acids were important carbon sources needed for efficient phosphorus and nitrogen removal from sewage. Five different fermentation media were configured for this experiment. In order to screen out the highly fermentable sugarcane molasses culture medium and its corresponding specific strain, six strains of lactic acid bacteria were used to perform fermentation in these media. The amount of acid produced in the fermentation broth was determined by intermittent titration. The contents of lactic acid, acetic acid, and formic acid in the fermentation broth were determined by HPLC, respectively. The rate of sugar consumption performed by each strain was determined by DNS method. The results showed that the acid production of lactic acid bacteria reached above 100 g/L in different media. Among the consumption rations of sugars performed by the six strains in different media,the highest consumption ration was obtained by the strain MX4 followed by the strain SN2 in the fermentation medium composed by the 3︰1 ratio of sugarcane molasses to glucose. Based on the results from HPLC, strain SN2 was more suitable for the production of lactic acid from sugarcane molasses, and strain MX4 was more suitable for the production of d C_2-C_5 organic acids. These two strains could be selected as potential industrial strains.
引文
[1]MARTINEZ F A C,BALCIUNAS E M,SALGADO JM,et al.Lactic acid properties,applications and production:A review[J].Trends in Food Science&Technology,2013,30(1):70-83.
[2]TANG J,WANG X C,HU Y,et al.Nitrogen remova enhancement using lactic acid fermentation products from food waste as external carbon sources:Performance and microbia communities[J].Bioresoure Technology,2018,56:259-268.
[3]刘智晓,LARSEN S B,PETERSEN G,等.污泥作为污水厂内碳源的水解特性及工艺选择[J].中国给水排水,2011,27(22):30-35.
[4]张艳梅,王晓慧,于浩强,等.糖蜜的应用研究进展[J].化学工业与工程技术,2011,32(6):28-31.
[5]秦菊霞,苏萍,黄谷亮,等.甘蔗糖蜜用米根霉发酵产乳酸的研究[J].食品工业,2008,29(5):44-46.
[6]陆琦,张穗生,吴仁智,等.三株甘蔗糖蜜酒精发酵高产酵母菌株的筛选[J].广西科学,2010,17(4):98-102.
[7]明恒磊.乳酸细菌Lc-1菌株L-乳酸发酵和提纯工艺[D].青岛:青岛科技大学,2012.
[8]于颖,刘杰,陈学玺,等.异型乳酸菌2种常用鉴定方法的比较[J].食品与发酵工业,2011,37(1):102-105.
[9]郑志,姜绍通,潘丽军,等.反相高效液相色谱法测定发酵液中乳酸的含量[J].食品科学,2003,24(12):89-91.
[10]白冬梅,班睿,赵学明,等.反相高效液相色谱法测定米根霉乳酸发酵液中的乳酸[J].色谱,2000,18(6):527-528.
[11]马瑞,欧阳嘉,李鑫,等.高效液相色谱法同时测定生物质乳酸发酵液中有机酸及糖类化合物[J].色谱,2012,30(1):62-66.
[12]胡锡阶,肖建辉,肖瑜,等.3,5-二硝基水杨酸比色法定糖的条件优化[J].遵义医学院学报,2005(1):9-11.
[13]KUMAR A,CHAE P S.Pyreneamide-based dipodal probes for ultra-sensitive and selective detection of 3,5-dinitrosalicylic acid in an aqueous solution[J].Dyes and Pigments,2017,147:400-412.
[14]黄靖华,孙靓,吴军华,等.甘蔗糖蜜发酵生产L-乳酸工艺条件研究[J].广西科学,2011,18(3):273-277.
[15]李志春,郭海蓉,王国梅,等.磷酸法预处理对甘蔗糖蜜中还原糖含量的影响研究[J].食品科技,2009,34(12):98-100.
[2]TANG J,WANG X C,HU Y,et al.Nitrogen remova enhancement using lactic acid fermentation products from food waste as external carbon sources:Performance and microbia communities[J].Bioresoure Technology,2018,56:259-268.
[3]刘智晓,LARSEN S B,PETERSEN G,等.污泥作为污水厂内碳源的水解特性及工艺选择[J].中国给水排水,2011,27(22):30-35.
[4]张艳梅,王晓慧,于浩强,等.糖蜜的应用研究进展[J].化学工业与工程技术,2011,32(6):28-31.
[5]秦菊霞,苏萍,黄谷亮,等.甘蔗糖蜜用米根霉发酵产乳酸的研究[J].食品工业,2008,29(5):44-46.
[6]陆琦,张穗生,吴仁智,等.三株甘蔗糖蜜酒精发酵高产酵母菌株的筛选[J].广西科学,2010,17(4):98-102.
[7]明恒磊.乳酸细菌Lc-1菌株L-乳酸发酵和提纯工艺[D].青岛:青岛科技大学,2012.
[8]于颖,刘杰,陈学玺,等.异型乳酸菌2种常用鉴定方法的比较[J].食品与发酵工业,2011,37(1):102-105.
[9]郑志,姜绍通,潘丽军,等.反相高效液相色谱法测定发酵液中乳酸的含量[J].食品科学,2003,24(12):89-91.
[10]白冬梅,班睿,赵学明,等.反相高效液相色谱法测定米根霉乳酸发酵液中的乳酸[J].色谱,2000,18(6):527-528.
[11]马瑞,欧阳嘉,李鑫,等.高效液相色谱法同时测定生物质乳酸发酵液中有机酸及糖类化合物[J].色谱,2012,30(1):62-66.
[12]胡锡阶,肖建辉,肖瑜,等.3,5-二硝基水杨酸比色法定糖的条件优化[J].遵义医学院学报,2005(1):9-11.
[13]KUMAR A,CHAE P S.Pyreneamide-based dipodal probes for ultra-sensitive and selective detection of 3,5-dinitrosalicylic acid in an aqueous solution[J].Dyes and Pigments,2017,147:400-412.
[14]黄靖华,孙靓,吴军华,等.甘蔗糖蜜发酵生产L-乳酸工艺条件研究[J].广西科学,2011,18(3):273-277.
[15]李志春,郭海蓉,王国梅,等.磷酸法预处理对甘蔗糖蜜中还原糖含量的影响研究[J].食品科技,2009,34(12):98-100.
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