海马补肾丸滋阴补肾作用研究
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摘要
目的采用多种实验模型评价海马补肾丸滋阴补肾作用,为临床提供实验依据。方法采用im氢化可的松致小鼠肾阳虚模型,观察海马补肾丸抗阳虚作用;采用ig甲状腺素加利血平致小鼠肾阴虚模型,观察海马补肾丸抗阴虚作用;采用im氢化可的松致大鼠肾阳虚模型,观察海马补肾丸对勃起功能的影响;采用去势雄性大鼠模型及正常幼龄雄性大鼠连续ig给药,观察海马补肾丸雄性激素样作用。结果给药10d,海马补肾丸1.1、2.2g/kg明显增加肾阳虚小鼠的自主活动次数,延长低温游泳时间,增加胸腺、脾脏的脏器指数;明显减少肾阴虚小鼠跳台错误次数、延长错误潜伏期,明显提高小鼠痛阈,增加小鼠胸腺、脾脏的脏器指数。给药2周,海马补肾丸0.56、1.12 g/kg明显增加大鼠bR/rR值、海绵体内压,明显改善勃起功能;明显增加去势大鼠血清睾酮(T)水平及前列腺、包皮腺、提肛肌的脏器指数;明显增加正常幼龄大鼠T、促卵泡激素(FSH)水平及精液囊的脏器指数。结论海马补肾丸具有明显的滋阴补肾作用。
Objective To evaluate the effect of Haima Bushen Pills(HBP) on nourishing yin and reinforcing kidney, and study the mechanism of the action with aim to provide the experimental evidence for the clinic. Methods Rats model with kidney-yang deficiency was induced by hydrocortisone through intramuscular injection to observe the anti kidney-yang deficiency effect of HBP.The effect of HBP against kidney-yin deficiency was observed using the kidney-yin deficiency model induced by oral administration of thyroxine and reserpine. The effect of HBP on erectile function was observed by intramuscular injection of hydrocortisone in rats.Castrated male rats and normal young male rats were administered orally to observe the androgenic effect of HBP. Results After 10 d of administration, HBP at dose of 1.1 g/kg and 2.2 g/kg significantly increased the number of autonomic activities of kidney-yang deficiency mice, extended the duration of low-temperature swimming, and increased the organ coefficients of thymus and spleen. It also significantly reduced the number of platform jumping errors and prolonged the incubation period of errors, significantly increased the pain threshold and increased the organ coefficients of thymus and spleen in mice with kidney-yin deficiency. After 2 weeks of administration, HBP at doses of 0.56 g/kg and 1.12 g/kg significantly increased the bR/rR ratio and intravitreal pressure in rats,significantly improved the erectile function, significantly increased the serum testosterone(T) concentration and the viscera coefficients of prostate, foreskin gland and levator ANI of castrated rats. The serum concentration of testosterone(T),follicle-stimulating hormone(FSH) and viscera index of seminal vesicle were significantly increased in normal young rats after administration of HBP. Conclusion HBP has obvious effect on nourishing yin and reinforcing kidney.
Objective To evaluate the effect of Haima Bushen Pills(HBP) on nourishing yin and reinforcing kidney, and study the mechanism of the action with aim to provide the experimental evidence for the clinic. Methods Rats model with kidney-yang deficiency was induced by hydrocortisone through intramuscular injection to observe the anti kidney-yang deficiency effect of HBP.The effect of HBP against kidney-yin deficiency was observed using the kidney-yin deficiency model induced by oral administration of thyroxine and reserpine. The effect of HBP on erectile function was observed by intramuscular injection of hydrocortisone in rats.Castrated male rats and normal young male rats were administered orally to observe the androgenic effect of HBP. Results After 10 d of administration, HBP at dose of 1.1 g/kg and 2.2 g/kg significantly increased the number of autonomic activities of kidney-yang deficiency mice, extended the duration of low-temperature swimming, and increased the organ coefficients of thymus and spleen. It also significantly reduced the number of platform jumping errors and prolonged the incubation period of errors, significantly increased the pain threshold and increased the organ coefficients of thymus and spleen in mice with kidney-yin deficiency. After 2 weeks of administration, HBP at doses of 0.56 g/kg and 1.12 g/kg significantly increased the bR/rR ratio and intravitreal pressure in rats,significantly improved the erectile function, significantly increased the serum testosterone(T) concentration and the viscera coefficients of prostate, foreskin gland and levator ANI of castrated rats. The serum concentration of testosterone(T),follicle-stimulating hormone(FSH) and viscera index of seminal vesicle were significantly increased in normal young rats after administration of HBP. Conclusion HBP has obvious effect on nourishing yin and reinforcing kidney.
引文
[1]姜华,王永洲,胡君茹.金刚酒对肾阳虚大、小鼠模型的影响[J].中国实验方剂学杂志,2011,17(8):211-214.
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[3]朱萱萱,吴旭同,倪文彭,等.补肾活血方对肾阴虚小鼠的抗应激作用的实验研究[J].中国医药导刊,2009,11(7):1172-1173.
[4]崔建美,马树祥,孙娜,等.针刺小鼠后三里对热水、冰水甩尾痛阈的影响[J].时珍国医国药,2009,20(7):1666-1667.
[5]王先进,沈周俊,张敏光,等.电刺激阴茎海绵体神经测定大鼠勃起功能的方法探讨[J].临床泌尿外科杂志,2012,27(2):228-232.
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[8]胡正远,孟艳梅,周雪梅.中医实验动物阴虚证造模方法研究[J].中医药临床杂志,2015,27(10):1395-1397.
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[10]张嘉妮,李玉星,杨梦琳,等.六味地黄汤及“补泻”药对对肾阴虚模型小鼠下丘脑-垂体-性腺轴功能的影响[J].中国现代应用药学,2017,34(1):25-29.
[11]傅万山,杨解人,丁伯平,等.六味地黄丸对肾上腺皮质激素型肾阴虚小鼠的药效学研究[J].皖南医学院学报,2002,21(1):11-15.
[12]李天禹,何清湖,卢芳国,等.雄性“肾阳虚”亚健康大鼠模型的研究[J].湖南中医药大学学报,2011,31(1):22-24.
[13]马晓宇,姜梦如,张涛,等.氢化可的松诱导大鼠肾阳虚模型评价[J].中国兽医学报,2017,37(9):1763-1765.
[14]陆茵,陈文星,华永庆,等.海马壮阳软胶囊温肾壮阳的实验药理学研究[J].南京中医药大学学报:自然科学版,2001,17(2):99-101.
[15]韦桂宁,周军,李茂,等.益元酒壮阳及免疫调节作用的实验研究[J].云南中医中药杂志,2010,31(8):70-71.
[16]王朝霞,邵玉宇,李擎,等.公驼宝克对肾阳虚大鼠的影响[J].内蒙古农业大学学报,2012,33(4):266-271.
[17]王清,朱凡河,司端运.归仙口服液对雄性去势大鼠性功能的影响[J].中国临床康复,2002,6(19):2940-2941.
[18]曾金雄,许家杰.针灸调节下丘脑-垂体-性腺轴功能的研究[J].中国性科学,2001,10(4):22-24.
[2]郝志红,张剑宇,赵思俊.补肾方对肾阳虚模型小鼠影响的实验研[J].山西中医,2008,24(3):42-44.
[3]朱萱萱,吴旭同,倪文彭,等.补肾活血方对肾阴虚小鼠的抗应激作用的实验研究[J].中国医药导刊,2009,11(7):1172-1173.
[4]崔建美,马树祥,孙娜,等.针刺小鼠后三里对热水、冰水甩尾痛阈的影响[J].时珍国医国药,2009,20(7):1666-1667.
[5]王先进,沈周俊,张敏光,等.电刺激阴茎海绵体神经测定大鼠勃起功能的方法探讨[J].临床泌尿外科杂志,2012,27(2):228-232.
[6]葛争艳,金龙,刘建勋.五子衍宗丸补肾壮阳作用的实验研究[J].中国实验方剂学杂志,2010,16(7):173-176.
[7]秦文艳,陈贺,朱竟赫,等,不同氢化可的松小鼠肾阳虚模型制备方法对比研究[J].实验动物学,2017,34(4):11-14.
[8]胡正远,孟艳梅,周雪梅.中医实验动物阴虚证造模方法研究[J].中医药临床杂志,2015,27(10):1395-1397.
[9]黄文慧,张莉莉,郭伊霖,等.肾阴虚证模型的构建及实验研究进展[J].中国药业,2017,26(10):1-7.
[10]张嘉妮,李玉星,杨梦琳,等.六味地黄汤及“补泻”药对对肾阴虚模型小鼠下丘脑-垂体-性腺轴功能的影响[J].中国现代应用药学,2017,34(1):25-29.
[11]傅万山,杨解人,丁伯平,等.六味地黄丸对肾上腺皮质激素型肾阴虚小鼠的药效学研究[J].皖南医学院学报,2002,21(1):11-15.
[12]李天禹,何清湖,卢芳国,等.雄性“肾阳虚”亚健康大鼠模型的研究[J].湖南中医药大学学报,2011,31(1):22-24.
[13]马晓宇,姜梦如,张涛,等.氢化可的松诱导大鼠肾阳虚模型评价[J].中国兽医学报,2017,37(9):1763-1765.
[14]陆茵,陈文星,华永庆,等.海马壮阳软胶囊温肾壮阳的实验药理学研究[J].南京中医药大学学报:自然科学版,2001,17(2):99-101.
[15]韦桂宁,周军,李茂,等.益元酒壮阳及免疫调节作用的实验研究[J].云南中医中药杂志,2010,31(8):70-71.
[16]王朝霞,邵玉宇,李擎,等.公驼宝克对肾阳虚大鼠的影响[J].内蒙古农业大学学报,2012,33(4):266-271.
[17]王清,朱凡河,司端运.归仙口服液对雄性去势大鼠性功能的影响[J].中国临床康复,2002,6(19):2940-2941.
[18]曾金雄,许家杰.针灸调节下丘脑-垂体-性腺轴功能的研究[J].中国性科学,2001,10(4):22-24.
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