基于肝失疏泄理论探讨正常人体氧化衰老进程的研究
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摘要
目的:探讨肝失疏泄在正常人体衰老进程中的影响作用,为阐释肝在衰老进程中的重要地位提供科学依据。方法:采用病例对照研究,根据艾森克人格问卷(EPQ)得分,确定高神经质和低神经质受试者各30例,应用酶联免疫吸附实验方法(ELISA)测定研究对象尿中8-羟基脱氧鸟苷(8-OHdG)与血浆中脂质过氧化产物(LPO)、超氧化物歧化酶(SOD)含量。结果:与低神经质组相比,高神经质组尿8-OHdG与血浆中LPO和SOD水平均显著升高(P<0.05);EPQ中神经质项目得分与8-OHdG(r_s=0.318)和LPO(r_s=0.331)之间存在明显的正相关(P<0.05),与SOD间相关性无统计学意义(P>0.05)。结论:肝失疏泄能够加速人衰老进程,其机制可能与肝失疏泄引发情绪应激进而产生氧化应激有关。
Objective: To investigate the effects ofliver failing to dispersein the aging process of normal humansand provide a scientific basis for explaining the important role of liver in the aging process. Methods: A case-control study was conducted. According to the EPQ score, 30 patients with high neuroticism and low neuroticism were identifiedrespectively.Urine8-hydroxy-2 deoxyguanosine(8-OHdG),lipid peroxidation product(LPO) and superoxide dismutase(SOD) in plasma were determined by enzyme-linked immunosorbent assay(ELISA). Results: Compared with the low neurotic group, 8-OHdG,LPO and SOD in the high neurotic group were significantly higher(P<0.05). A significant positive correlation was found between neurotic item scores in EPQ and 8-OHdG(r_s=0.318) as well as LPO(r_s=0.331).Conclusion:If liver fails to disperse,it can accelerate the aging process, and the mechanism may be related tooxidative stress caused bythe emotional stress.
Objective: To investigate the effects ofliver failing to dispersein the aging process of normal humansand provide a scientific basis for explaining the important role of liver in the aging process. Methods: A case-control study was conducted. According to the EPQ score, 30 patients with high neuroticism and low neuroticism were identifiedrespectively.Urine8-hydroxy-2 deoxyguanosine(8-OHdG),lipid peroxidation product(LPO) and superoxide dismutase(SOD) in plasma were determined by enzyme-linked immunosorbent assay(ELISA). Results: Compared with the low neurotic group, 8-OHdG,LPO and SOD in the high neurotic group were significantly higher(P<0.05). A significant positive correlation was found between neurotic item scores in EPQ and 8-OHdG(r_s=0.318) as well as LPO(r_s=0.331).Conclusion:If liver fails to disperse,it can accelerate the aging process, and the mechanism may be related tooxidative stress caused bythe emotional stress.
引文
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[3] Kirkwood TB.A systematic look at an old problem[J].Nature.2008,451(7179):644-647.
[4] Steves CJ,Spector TD,Jackson SH.Ageing,genes,environment and epigenetics:what twin studies tell us now,and in the future[J].Age Ageing,2012,41(5):581-586.
[5] Koenig HG.Religion and Mental Health[M].London:Nikki Levy,2018:79-102.
[6] 宋昊翀,孙冉冉,张惠敏,等.衰老的中医理论研究[J].中华中医药杂志,2015,30(6):1889-1893.
[7] 韩景献.“三焦气化失常-衰老”相关论[J].中医杂志,2008(3):200-202
[8] 王静淑.延缓衰老从瘀论治[J].中国中医基础医学杂志,1998(8):47-50.
[9] 梁治学,胡燕,李其忠,等.“衰老”词源学探析[J].中国老年学杂志,2015,35(22):6619-6620.
[10] 黄斌,刘仍海,刘薇,等.单味中药抗衰老研究进展[J].中华中医药学刊,2016,34(12):2874-2877.
[11] Weston SJ,Jackson JJ.The role of vigilance in the relationship between neuroticism and health:A registered report[J].Journal of Research in Personality,2017(73):27-34.
[12] Ladislav Volicer.Behavioral Problems and Dementia[J].Clinics in Geriatric Medicine,2018,34(4):637-651.
[13] Navrady LB,Ritchie S,Chan S,et al.Intelligence and neuroticism in relation to depression and psychologica ldistress:Evidence from two large population cohorts[J].EurPsychiatry,2017,43:58-65.
[14] Steffens DC,Wang L,Manning KJ,et al.Negative Affectivity,Aging,and Depression:Results From the Neurobiology of Late-Life Depression(NBOLD)Study[J].Eurpsychiatry,2017,25(10):1135-1149.
[15] Chapman BP,Benedict RH,Lin F,et al.Personality and Performance in Specific Neurocognitive Domains Among Older Persons[J].American Journal of Geriatric Psychiatry,2017,25(8):900-908.
[16] 刘焕兰,郑先贞.衰老机理的五脏相关性探讨[J].中医杂志,2010,42(3):6-8.
[17] 沈玮,陈霞,詹向红.肝主疏泄功能与MCI相关性理论探讨[J].中华中医药学刊,2017,35(4):870-872.
[18] 孙英霞.情志致病方式与伤脏规律研究[D].济南:山东中医药大学,2009.
[19] 张翠利,付丽娜,杨小云,等.活性氧自由基与细胞衰老关系的研究进展[J].广州化工,2015,43(19):5-7.
[20] Detienne G,De WH,Mergan L,et al.Beyond ROS clearance:Peroxiredoxins in stress signaling and aging[J].Free Radical Biology and Medicine,2018,44:33-48.
[21] Giorgi G,Marchi S,Simoes ICM,et al.Mitochondria and Reactive Oxygen Species in Aging and Age-Related Diseases[M].International Review of Cell & Molecular Biology,2018,340:209-344.
[22] Wu D,Liu B,Yin J,et al.Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS[J].Journal of Chromatography B,2017,1064:1-6.
[23] Baciou L,Masoud R,Souabni H,et al.Phagocyte NADPH oxidase,oxidative stress and lipids:Anti- or pro ageing?[J].Mechanisms of Ageing and Development,2018,172:30-34.
[24] Lam VYY,Raineki C,Wang LY,et al.Role of corticosterone in anxiety- and depressive-like behavior and HPA regulation following prenatal alcohol exposure[J].Progress in Neuro-Psychopharmacology and Biological Psychiatry,2019,90:1-15.
[25] Zhang L,Zhou RL,Li XX,et al.Stress-induced change of mitochondria membrane potential regulated by genomic and non-genomic GR signaling:A possible mechanism for hippocampus atrophy in PTSD[J].Medical Hypotheses,2006,66(6):1205-1208.
[26] Lopez-Gonzalez R,Lu Y,Gendron TF.Poly(GR) in C9ORF72-Related ALS/FTD Compromises Mitochondrial Function and Increases Oxidative Stress and DNA Damage in iPSC-Derived Motor Neurons[J].Neuron,2016,92(2):383-391.
[27] Meraz-Ríos MA,Franco-Bocanegra D,Rios DT,et al.Early Onset Alzheimer's Disease and Oxidative Stress[J].Oxidative Medicine&Cellular Longevity,2014,2014 (10):375968.
[28] Coughlin JM,Hayes LN,Tanaka T,et al.Reduced superoxide dismutase-1 (SOD1) in cerebrospinal fluid of patients with early psychosis in association with clinical features[J].Schizophrenia Research,2017,183:64-69.