中医药对椎间盘退变相关信号通路影响实验研究进展
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摘要
目的:综述近年来中医药对椎间盘退变相关信号通路影响的实验研究进展。方法:查阅近年来公开发表的相关文献,从信号通路受抑制后延缓椎间盘退变和激活后延缓椎间盘退变两方面,分别总结中药提取物、复方、针刺及手法等影响椎间盘退变的作用机制。结果:中医药的相关研究主要集中在NF-κB、MAPK及Wnt等经典信号通路。施加因素多取自临床常用的经方、验方,尤以补益肝肾及温补肾阳类为主。并对椎间盘退变模型均取得了较好的实验结果。结论:通过对信号通路影响的实验研究,在分子层面阐释了中医药对椎间盘退变影响的作用机制。但研究仍存在一定局限性,需继续深入进行,为实现椎间盘退变的中医药精准医疗打下更坚实的基础。
Objective:To summarize the recent traditional Chinese medicine researches on signaling pathways which were related to degenerative disc disease.Method:Wecollected the related researches from the recent yearsand summarized from 3 aspects: Chinese herbology, prescription and acupuncture.Results:Most studies focused on the 3 classical signaling pathways:NF-κB, MAPK and Wnt. The experimental factors were chosen from the classical prescription, mostly for nourishing liver and kidney or warming and tonifyingkidney Yang, and good results are obtained for degenerative models under laboratory conditions.Conclusion:Through the studies of signaling pathways, the mechanism of traditional Chinese medicine treatments for degenerative disc disease is further elaborated at molecular level. But limitations still existand therelevant experiments still need to be deeplycarried on in order to perfect the theoretical system of traditional Chinese medicine at the molecular leveland prepare for the realization of traditional Chinese medicine precision medicine.
Objective:To summarize the recent traditional Chinese medicine researches on signaling pathways which were related to degenerative disc disease.Method:Wecollected the related researches from the recent yearsand summarized from 3 aspects: Chinese herbology, prescription and acupuncture.Results:Most studies focused on the 3 classical signaling pathways:NF-κB, MAPK and Wnt. The experimental factors were chosen from the classical prescription, mostly for nourishing liver and kidney or warming and tonifyingkidney Yang, and good results are obtained for degenerative models under laboratory conditions.Conclusion:Through the studies of signaling pathways, the mechanism of traditional Chinese medicine treatments for degenerative disc disease is further elaborated at molecular level. But limitations still existand therelevant experiments still need to be deeplycarried on in order to perfect the theoretical system of traditional Chinese medicine at the molecular leveland prepare for the realization of traditional Chinese medicine precision medicine.
引文
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[17] 鲁花,于露,甄欢欢,等.人参皂苷Rg1通过抑制Wnt/β-catenin通路促进退变人腰椎间盘髓核细胞的生长及胞外基质合成[J].中国病理生理杂志,2018,34(4):705-710.
[18] 谢芳,吴官保,姚红艳,等.补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究[J].湖南中医药大学学报,2018,38(3):275-279.
[19] 夏炳江,侯明生,张磊,等.五福饮对大鼠尾部退变椎间盘的影响及其作用机制[J].中医正骨,2018,30(1):18-24.
[20] 刘伯龄,陈齐勇,刘少强,等.独活寄生汤水提物对退变大鼠椎间盘软骨细胞功能的影响[J].福建中医药,2018,49(1):17-20.
[21] 廖军,谢巧瑜,张乐,等.电针对颈椎病模型大鼠椎间盘纤维环细胞Wnt-β-catenin信号通路的影响[J].中国针灸,2014,34(12):1203-1207.
[22] 刘汝银,彭晓艳,岳宗进,等.当归对大鼠椎间盘髓核细胞自噬及氧化应激损伤的作用及机制研究[J].中国脊柱脊髓杂志,2018,28(2):176-182.
[23] 徐永明,徐宏光,高智,等.淫羊藿素通过抑制Hedgehog信号通路保护终板软骨细胞退变[J].中国临床药理学与治疗学,2017,22(4):373-380.
[24] 童秀冰,郑佳璇,廖军,等.电针对颈椎病大鼠椎间盘软骨细胞及PI3K/Akt信号通路的影响[J].中国中医基础医学杂志,2016,22(9):1232-1235.
[2] Cui X,Trinh K,Wang YJ.Chinese herbal medicine for chronic neck pain due to cervical degenerative disc disease[J].Cochrane Database of Systematic Reviews,2010(1):CD006556.
[3] Li Y.Cordycepin inhibits LPS-induced inflammatory and matrix degradation in the intervertebral disc[J].PeerJ,2016(4):e1992.
[4] Li Y,Li K,Hu Y,et al.Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc[J].International Journal of Clinical & Experimental Pathology,2015,8(6):6203.
[5] Liu ZC,Wang ZL,Huang CY,et al.Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF-κB pathway[J].Acta Pharmacol Sin,2018,39(6):912-922.
[6] 陈莎,王诗忠,邓德万.身痛逐瘀汤对腰椎退变模型大鼠纤维环细胞p38MAPK信号通路的影响[J].康复学报,2015,25(4):22-26.
[7] 王先海,李珍斌,李小妹,等.通经定晕丸阻断颈椎病大鼠NF-κB激活MCP-1/CCR2通路及TNF-α及IL-1β、IL-6表达的研究[J].中国临床药理学与治疗学,2018,23(4):370-376.
[8] 仇湘中,朱承勋,张信成,等.补肝健腰方对腰椎间盘退变大鼠椎间盘TNF-α及NF-κB表达的影响[J].中国中医骨伤科杂志,2017,25(9):9-11,16.
[9] 何升华,谭伟伟,孙志涛,等.腰突颗粒对人髓核细胞核因子κB信号通路的影响[J].中国组织工程研究,2016,20(33):4933-4939.
[10] 柳根哲,孙旗,陈江,等.高静水压下益气活血汤通过p38MAPK信号通路对兔椎体终板软骨细胞的调控作用[J].现代中西医结合杂志,2018,27(10):1027-1030,1034.
[11] 杨届,吴官保,冯帅华,等.补肾活血汤对去势后大鼠腰椎间盘p38MAPK蛋白含量影响的研究[J].中医药导报,2017,23(2):17-20.
[12] 周江涛,王庆来,赵依娜,等.独活寄生汤及其拆方干预腰椎间盘纤维环细胞信号通路的研究[J].福建中医药,2012,43(3):45-48.
[13] 徐无忌,刘思华,杨康.六味地黄丸对椎间盘体外退变模型髓核细胞MAPK信号级联的影响[J].中国组织工程研究,2017,21(12):1861-1866.
[14] 李志元,张丹,杨延婷,等.基于MAPKs信号通路的针灸镇痛作用机制研究进展[J].中华中医药学刊,2017,35(12):3005-3009.
[15] Kondo N.Intervertebral Disc Development Is Regulated by Wnt/β-catenin Signaling[J].Spine,2011,36(8):E513-E518.
[16] 廖军,谢巧瑜,张乐,等.捏脊疗法对颈椎病模型大鼠纤维环细胞影响的实验研究[J].中国中医基础医学杂志,2014,20(5):611-614.
[17] 鲁花,于露,甄欢欢,等.人参皂苷Rg1通过抑制Wnt/β-catenin通路促进退变人腰椎间盘髓核细胞的生长及胞外基质合成[J].中国病理生理杂志,2018,34(4):705-710.
[18] 谢芳,吴官保,姚红艳,等.补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究[J].湖南中医药大学学报,2018,38(3):275-279.
[19] 夏炳江,侯明生,张磊,等.五福饮对大鼠尾部退变椎间盘的影响及其作用机制[J].中医正骨,2018,30(1):18-24.
[20] 刘伯龄,陈齐勇,刘少强,等.独活寄生汤水提物对退变大鼠椎间盘软骨细胞功能的影响[J].福建中医药,2018,49(1):17-20.
[21] 廖军,谢巧瑜,张乐,等.电针对颈椎病模型大鼠椎间盘纤维环细胞Wnt-β-catenin信号通路的影响[J].中国针灸,2014,34(12):1203-1207.
[22] 刘汝银,彭晓艳,岳宗进,等.当归对大鼠椎间盘髓核细胞自噬及氧化应激损伤的作用及机制研究[J].中国脊柱脊髓杂志,2018,28(2):176-182.
[23] 徐永明,徐宏光,高智,等.淫羊藿素通过抑制Hedgehog信号通路保护终板软骨细胞退变[J].中国临床药理学与治疗学,2017,22(4):373-380.
[24] 童秀冰,郑佳璇,廖军,等.电针对颈椎病大鼠椎间盘软骨细胞及PI3K/Akt信号通路的影响[J].中国中医基础医学杂志,2016,22(9):1232-1235.
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